BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cocculus23_contig00006251
         (1115 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss...   421   e-115
gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]                    418   e-114
sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltN...   339   1e-90
emb|CBI31830.3| unnamed protein product [Vitis vinifera]              308   2e-81
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   306   1e-80
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]   306   1e-80
ref|XP_002274530.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy...   305   3e-80
emb|CAN62646.1| hypothetical protein VITISV_013218 [Vitis vinifera]   305   3e-80
ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy...   304   4e-80
emb|CBI34714.3| unnamed protein product [Vitis vinifera]              303   7e-80
ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   303   7e-80
emb|CBI34715.3| unnamed protein product [Vitis vinifera]              302   2e-79
ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   301   4e-79
ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   294   4e-77
gb|EXC34114.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 [...   293   9e-77
ref|XP_003631391.1| PREDICTED: LOW QUALITY PROTEIN: (S)-N-methyl...   290   8e-76
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi...   290   8e-76
ref|XP_002274586.2| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy...   289   2e-75
gb|EXC34118.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [...   288   3e-75
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla...   288   4e-75

>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
          Length = 486

 Score =  421 bits (1083), Expect = e-115
 Identities = 207/310 (66%), Positives = 252/310 (81%), Gaps = 1/310 (0%)
 Frame = +2

Query: 2    VFSKDVFVFDDE-DRIGMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKV 178
            VFSKDVFV+ DE D+ G+Q+LI+E+LMIGA PN+AEFYP LE LDLQGLK++C +R  +V
Sbjct: 185  VFSKDVFVYGDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDERFIRV 244

Query: 179  NKLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETSS 358
             K+WE TVK                  KDMLDVLL N+F D QIN LFLETFGPGSETSS
Sbjct: 245  MKMWEGTVKERKANRNEES--------KDMLDVLLANDFNDAQINALFLETFGPGSETSS 296

Query: 359  ATIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAP 538
            ATIEW +AELIK+P +++KVR+EL++VVG  S +KE+ +P LPYLQACIKE MRLHPAAP
Sbjct: 297  ATIEWVIAELIKSPKEMAKVRKELNEVVGT-STIKESDLPQLPYLQACIKEAMRLHPAAP 355

Query: 539  FLLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNH 718
            FLLP RA ETC+VMGYT+PK+ +++VNAYAIGRDP +W DPSTFWPERFLES+VD++G H
Sbjct: 356  FLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDVDFHGAH 415

Query: 719  FEFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAID 898
            ++FIPFGSGRR CVG+PLA RTIPLIV SL+HN+ + LP   RPE+L MNE+LSL+LAID
Sbjct: 416  YQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLTLAID 475

Query: 899  PSLSIIPKVR 928
            PSL ++PK R
Sbjct: 476  PSLCVVPKAR 485


>gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]
          Length = 346

 Score =  418 bits (1075), Expect = e-114
 Identities = 210/309 (67%), Positives = 245/309 (79%)
 Frame = +2

Query: 2   VFSKDVFVFDDEDRIGMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVN 181
           +FSKDVF +   D   MQ+LIKE+LMIGA+PNLAEF+PI E  DLQGLK+RC  R  +V 
Sbjct: 46  IFSKDVFRYVGADGDKMQTLIKEMLMIGAAPNLAEFWPIFEEFDLQGLKKRCNQRFSEVM 105

Query: 182 KLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETSSA 361
           K+W+ TV+                  KDMLDVLL N+F D QIN +FLETFGPGSETSSA
Sbjct: 106 KMWKGTVEERKVKRNEEP--------KDMLDVLLLNDFNDAQINAVFLETFGPGSETSSA 157

Query: 362 TIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPF 541
           T EWA+AELIKNP  L+KVRQELDQVVG KS VKE+ +PHLPYLQACIKETMRLHPAAPF
Sbjct: 158 TSEWALAELIKNPHILAKVRQELDQVVG-KSTVKESDLPHLPYLQACIKETMRLHPAAPF 216

Query: 542 LLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHF 721
           LLPHRA ETC VMGYT+PKD +++VN YAIGRDP TW +P+TF PERFLES+VDY G HF
Sbjct: 217 LLPHRAVETCPVMGYTIPKDTQVLVNIYAIGRDPKTWKEPTTFKPERFLESDVDYYGKHF 276

Query: 722 EFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAIDP 901
           EFIPFG+GRR CVG+PLA RTIPLIV++L+    WSLPD K P++L MNE LSLSLA+DP
Sbjct: 277 EFIPFGAGRRQCVGMPLATRTIPLIVSNLVQTLDWSLPDGKLPQDLVMNESLSLSLALDP 336

Query: 902 SLSIIPKVR 928
           +L++IPK R
Sbjct: 337 TLTVIPKSR 345


>sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltName:
            Full=(S)-N-methylcoclaurine oxidase [C-O
            phenol-coupling]; AltName: Full=CYPLXXX; AltName:
            Full=Cytochrome P450 80 gi|642386|gb|AAC48987.1|
            cytochrome P-450 CYP80 [Berberis stolonifera]
          Length = 487

 Score =  339 bits (869), Expect = 1e-90
 Identities = 168/310 (54%), Positives = 216/310 (69%), Gaps = 1/310 (0%)
 Frame = +2

Query: 2    VFSKDVFVFDDE-DRIGMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKV 178
            VFSKDVF + D+ D +GM  LI  +LM G   ++A ++P+L   DL GLKR+  ++   +
Sbjct: 185  VFSKDVFEYSDQSDEVGMDKLIHGMLMTGGDFDVASYFPVLARFDLHGLKRKMDEQFKLL 244

Query: 179  NKLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETSS 358
             K+WE  V                   KDMLDVL+ N+F + QIN +F+ETFGPGS+T+S
Sbjct: 245  IKIWEGEV--------LARRANRNPEPKDMLDVLIANDFNEHQINAMFMETFGPGSDTNS 296

Query: 359  ATIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAP 538
              IEWA+A+LIKNP KL+K+R+ELD+VVG  S VKE+H   LPYLQAC+KETMRL+P   
Sbjct: 297  NIIEWALAQLIKNPDKLAKLREELDRVVGRSSTVKESHFSELPYLQACVKETMRLYPPIS 356

Query: 539  FLLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNH 718
             ++PHR  ETCQVMGYT+PK   + VNA+AIGRDP  W DP  F PERFL+S+++YNG  
Sbjct: 357  IMIPHRCMETCQVMGYTIPKGMDVHVNAHAIGRDPKDWKDPLKFQPERFLDSDIEYNGKQ 416

Query: 719  FEFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAID 898
            F+FIPFGSGRRIC G PLA R IPL++ASL+H F W LPD    E+L M E+ +LSL + 
Sbjct: 417  FQFIPFGSGRRICPGRPLAVRIIPLVLASLVHAFGWELPDGVPNEKLDMEELFTLSLCMA 476

Query: 899  PSLSIIPKVR 928
              L +IPKVR
Sbjct: 477  KPLRVIPKVR 486


>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
          Length = 1538

 Score =  308 bits (790), Expect = 2e-81
 Identities = 148/312 (47%), Positives = 212/312 (67%), Gaps = 2/312 (0%)
 Frame = +2

Query: 2    VFSKDVFVFD--DEDRIGMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGK 175
            + S   F  D  D +R G++  I     +GA+PNL++FYPIL+GL+L G K++  + +G+
Sbjct: 1234 ILSNTFFSMDLCDFEREGLKDFIYRAAELGATPNLSDFYPILDGLNLHGSKKKSKEALGR 1293

Query: 176  VNKLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETS 355
            +   WE T+K                  +D+L+  L   F D QIN + LE F  G++TS
Sbjct: 1294 ILATWEGTLKERRKQKNPGSSH------RDLLEAFLEIRFEDDQINQVILELFSAGADTS 1347

Query: 356  SATIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAA 535
            + TIEWA+ +LI+NP  + K+R EL +++G +S V+E+H+PHLPYLQAC+KET+RLHP A
Sbjct: 1348 TLTIEWAITQLIRNPDVMYKLRDELTKIIG-ESPVRESHLPHLPYLQACVKETLRLHPPA 1406

Query: 536  PFLLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGN 715
            P LLPHRA ETCQVMGYT+PKD ++ VN +A+GRDP  W DP +F PERFL+S +++ GN
Sbjct: 1407 PLLLPHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPKVWDDPLSFTPERFLDSKLEFKGN 1466

Query: 716  HFEFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAI 895
             FE+IPFG+GRRIC G+ L  R +PL++A+L+H F WSLPD     ++ M E L ++L  
Sbjct: 1467 DFEYIPFGAGRRICPGMALGARQVPLVLATLVHLFDWSLPDNMDSAQIDMEEWLVITLRK 1526

Query: 896  DPSLSIIPKVRE 931
            +  L ++PKVR+
Sbjct: 1527 ENPLRLVPKVRK 1538



 Score =  307 bits (786), Expect = 6e-81
 Identities = 153/312 (49%), Positives = 208/312 (66%), Gaps = 2/312 (0%)
 Frame = +2

Query: 5    FSKDVFVFDDEDRIG--MQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKV 178
            FS D+  F+DE R+G  ++ LI +    GA+PNL+++YPIL GLD+QG+ R+  +   ++
Sbjct: 735  FSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFERI 794

Query: 179  NKLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETSS 358
               WE  +K                  +D L+ LL   F D QIN + LE F  G+ETSS
Sbjct: 795  PTTWEDILKERRTQRSNRSSH------RDFLEALLEIGFEDDQINQVILELFSAGAETSS 848

Query: 359  ATIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAP 538
             T+EWAMAELI+N   + K+R EL Q+VG +S V+E+H+P LPYLQAC+KE +RLHP AP
Sbjct: 849  LTVEWAMAELIRNQDAMDKLRGELRQIVG-ESPVRESHLPRLPYLQACVKEALRLHPPAP 907

Query: 539  FLLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNH 718
             LLPH A ETCQVMGYT+PKD +I VN +A+ RDP  W DP +F PERFL+S +D+ GN 
Sbjct: 908  LLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLSFKPERFLDSKLDFKGND 967

Query: 719  FEFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAID 898
            FE+IPFG+GRRIC GL L  R +PLI+A+ +H F WSLP      +L M E L ++L  +
Sbjct: 968  FEYIPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLPGNMDSAQLDMEEWLVITLRKE 1027

Query: 899  PSLSIIPKVREH 934
              L ++P+VR++
Sbjct: 1028 QPLRLVPRVRKY 1039



 Score =  282 bits (722), Expect = 2e-73
 Identities = 144/308 (46%), Positives = 199/308 (64%), Gaps = 2/308 (0%)
 Frame = +2

Query: 5    FSKDVFVFDDEDRIG--MQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKV 178
            FS D   F+  D  G  ++ L+ E   +GA+ N+ +FYPIL GLD+QG++++  +   ++
Sbjct: 195  FSIDFSDFEGRDVRGVLLKDLLNENAELGAT-NILDFYPILGGLDIQGIRKKLKEIFRRI 253

Query: 179  NKLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETSS 358
               WE  +K                   D LD LL   F D QIN + +E F  G ETSS
Sbjct: 254  PTTWEDILKERRKQRIHGSSHG------DFLDALLETGFEDDQINHVIMELFFAGPETSS 307

Query: 359  ATIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAP 538
             T+EWAMAELIKN   + K+  EL Q++G +S V+E+H+PHLPYLQAC+KET+RLHP  P
Sbjct: 308  LTVEWAMAELIKNQDAMHKLCNELTQIIG-ESPVRESHLPHLPYLQACVKETLRLHPTGP 366

Query: 539  FLLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNH 718
             LLPHRA ETCQ+MGYT+PKD  I VN +A+GRDP TW DP +F PERFL+S +++ GN 
Sbjct: 367  LLLPHRATETCQIMGYTIPKDSIIFVNMWAMGRDPGTWEDPLSFKPERFLDSKLEFKGND 426

Query: 719  FEFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAID 898
            FE+IPFG+GRR+C G+PLA R +P+I+A+ +  F WS P +    E+ M E   ++L  +
Sbjct: 427  FEYIPFGAGRRMCPGMPLAARLVPMILATFVRLFDWSTPGDMDFAEIDMEERFVITLRKE 486

Query: 899  PSLSIIPK 922
              L ++ K
Sbjct: 487  QPLRLLKK 494


>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
            [Vitis vinifera]
          Length = 498

 Score =  306 bits (784), Expect = 1e-80
 Identities = 153/311 (49%), Positives = 207/311 (66%), Gaps = 2/311 (0%)
 Frame = +2

Query: 5    FSKDVFVFDDEDRIG--MQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKV 178
            FS D+  F+DE R+G  ++ LI +    GA+PNL+++YPIL GLD+QG+ R+  +   ++
Sbjct: 195  FSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFERI 254

Query: 179  NKLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETSS 358
               WE  +K                  +D L+ LL   F D QIN + LE F  G+ETSS
Sbjct: 255  PTTWEDILKERRTQRSNRSSH------RDFLEALLEIGFEDDQINQVILELFSAGAETSS 308

Query: 359  ATIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAP 538
             T+EWAMAELI+N   + K+R EL Q+VG +S V+E+H+P LPYLQAC+KE +RLHP AP
Sbjct: 309  LTVEWAMAELIRNQDAMDKLRGELRQIVG-ESPVRESHLPRLPYLQACVKEALRLHPPAP 367

Query: 539  FLLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNH 718
             LLPH A ETCQVMGYT+PKD +I VN +A+ RDP  W DP +F PERFL+S +D+ GN 
Sbjct: 368  LLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLSFKPERFLDSKLDFKGND 427

Query: 719  FEFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAID 898
            FE+IPFG+GRRIC GL L  R +PLI+A+ +H F WSLP      +L M E L ++L  +
Sbjct: 428  FEYIPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLPGNMDSAQLDMEEWLVITLRKE 487

Query: 899  PSLSIIPKVRE 931
              L ++P+VR+
Sbjct: 488  QPLRLVPRVRK 498


>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
          Length = 498

 Score =  306 bits (784), Expect = 1e-80
 Identities = 153/311 (49%), Positives = 207/311 (66%), Gaps = 2/311 (0%)
 Frame = +2

Query: 5    FSKDVFVFDDEDRIG--MQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKV 178
            FS D+  F+DE R+G  ++ LI +    GA+PNL+++YPIL GLD+QG+ R+  +   ++
Sbjct: 195  FSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFERI 254

Query: 179  NKLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETSS 358
               WE  +K                  +D L+ LL   F D QIN + LE F  G+ETSS
Sbjct: 255  PTTWEDILKERRTQRSNRSSH------RDFLEALLEIGFEDDQINQVILELFSAGAETSS 308

Query: 359  ATIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAP 538
             T+EWAMAELI+N   + K+R EL Q+VG +S V+E+H+P LPYLQAC+KE +RLHP AP
Sbjct: 309  LTVEWAMAELIRNQDAMDKLRGELRQIVG-ESPVRESHLPRLPYLQACVKEALRLHPPAP 367

Query: 539  FLLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNH 718
             LLPH A ETCQVMGYT+PKD +I VN +A+ RDP  W DP +F PERFL+S +D+ GN 
Sbjct: 368  LLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLSFKPERFLDSKLDFKGND 427

Query: 719  FEFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAID 898
            FE+IPFG+GRRIC GL L  R +PLI+A+ +H F WSLP      +L M E L ++L  +
Sbjct: 428  FEYIPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLPGNMDSAQLDMEEWLVITLRKE 487

Query: 899  PSLSIIPKVRE 931
              L ++P+VR+
Sbjct: 488  QPLRLVPRVRK 498


>ref|XP_002274530.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2-like
            [Vitis vinifera]
          Length = 515

 Score =  305 bits (780), Expect = 3e-80
 Identities = 146/309 (47%), Positives = 209/309 (67%), Gaps = 2/309 (0%)
 Frame = +2

Query: 2    VFSKDVFVFD--DEDRIGMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGK 175
            + S   F  D  D +R G++  I     +GA+PNL++FYPIL+GL+L G K++  + +G+
Sbjct: 189  ILSNTFFSMDLCDFEREGLKDFIYRAAELGATPNLSDFYPILDGLNLHGSKKKSKEALGR 248

Query: 176  VNKLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETS 355
            +   WE T+K                  +D+L+  L   F D QIN + LE F  G++TS
Sbjct: 249  ILATWEGTLKERRKQKNPGSSH------RDLLEAFLEIRFEDDQINQVILELFSAGADTS 302

Query: 356  SATIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAA 535
            + TIEWA+ +LI+NP  + K+R EL +++G +S V+E+H+PHLPYLQAC+KET+RLHP A
Sbjct: 303  TLTIEWAITQLIRNPDVMYKLRDELTKIIG-ESPVRESHLPHLPYLQACVKETLRLHPPA 361

Query: 536  PFLLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGN 715
            P LLPHRA ETCQVMGYT+PKD ++ VN +A+GRDP  W DP +F PERFL+S +++ GN
Sbjct: 362  PLLLPHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPKVWDDPLSFTPERFLDSKLEFKGN 421

Query: 716  HFEFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAI 895
             FE+IPFG+GRRIC G+ L  R +PL++A+L+H F WSLPD     ++ M E L ++L  
Sbjct: 422  DFEYIPFGAGRRICPGMALGARQVPLVLATLVHLFDWSLPDNMDSAQIDMEEWLVITLRK 481

Query: 896  DPSLSIIPK 922
            +  L ++PK
Sbjct: 482  ENPLRLVPK 490


>emb|CAN62646.1| hypothetical protein VITISV_013218 [Vitis vinifera]
          Length = 475

 Score =  305 bits (780), Expect = 3e-80
 Identities = 147/309 (47%), Positives = 210/309 (67%)
 Frame = +2

Query: 5    FSKDVFVFDDEDRIGMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNK 184
            FS D+  F+ E   G++  I     +GA+PNL++FYPIL+GL+L G K++  + +G++  
Sbjct: 177  FSMDLCDFERE---GLKDFIYRAAELGATPNLSDFYPILDGLNLHGSKKKSKEALGRILA 233

Query: 185  LWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETSSAT 364
             WE T+K                  +D+L+  L   F D QIN + LE F  G++TS+ T
Sbjct: 234  TWEGTLKERRKQKNPGSSH------RDLLEAFLEIRFEDDQINQVILELFSAGADTSTLT 287

Query: 365  IEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFL 544
            IEWA+ +LI+NP  + K+R EL +++G +S V+E+H+PHLPYLQAC+KET+RLHP AP L
Sbjct: 288  IEWAITQLIRNPDVMYKLRDELTKIIG-ESPVRESHLPHLPYLQACVKETLRLHPPAPLL 346

Query: 545  LPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFE 724
            LPHRA ETCQVMGYT+PKD ++ VN +A+GRDP  W  P +F PERFL+S +++ GN FE
Sbjct: 347  LPHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPKVWDXPLSFTPERFLDSKLEFKGNDFE 406

Query: 725  FIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAIDPS 904
            +IPFG GRRIC G+ L  R +PL++A+L+H F WSLPD     ++ M E L ++L  +  
Sbjct: 407  YIPFGXGRRICPGMALGARQVPLVLATLVHLFDWSLPDNMDSAQIDMEEWLVITLRKENP 466

Query: 905  LSIIPKVRE 931
            L ++PKVR+
Sbjct: 467  LRLVPKVRK 475


>ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
            [Vitis vinifera]
          Length = 503

 Score =  304 bits (779), Expect = 4e-80
 Identities = 150/311 (48%), Positives = 212/311 (68%), Gaps = 1/311 (0%)
 Frame = +2

Query: 2    VFSKDVFVFDDEDRIG-MQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKV 178
            + S+D   F+D+++ G M+ L+++++M  A+PNL +FY I  GLDLQGL ++  + + ++
Sbjct: 200  LMSRDFISFEDDNKDGGMKGLVRKMVMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARI 259

Query: 179  NKLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETSS 358
              +WE  ++                  +D L++L+ + + D QIN LF+E    G++TSS
Sbjct: 260  CSMWESVIRERREGASDDPSK------QDFLNILIRSGYSDDQINQLFMELLTAGADTSS 313

Query: 359  ATIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAP 538
            +T+EWAMAELIK+P  + KV +EL + +      K + +PHLPYLQAC+KET+RLHP+AP
Sbjct: 314  STLEWAMAELIKSPESMKKVHEELAREISDNLP-KASDLPHLPYLQACVKETLRLHPSAP 372

Query: 539  FLLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNH 718
             LLP RA  +C+VM YT+PKD +I VNA+AIGRDP  W DP  F PERFL S VD+ GN+
Sbjct: 373  LLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNN 432

Query: 719  FEFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAID 898
             EFIPFG+GRRIC GLP+A R +PLI+ASL H F WSLP+   P+EL MN+   ++L  +
Sbjct: 433  LEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLPNGTTPDELDMNDKFGVTLQKE 492

Query: 899  PSLSIIPKVRE 931
              L IIPKVR+
Sbjct: 493  QPLLIIPKVRK 503


>emb|CBI34714.3| unnamed protein product [Vitis vinifera]
          Length = 936

 Score =  303 bits (777), Expect = 7e-80
 Identities = 150/311 (48%), Positives = 211/311 (67%), Gaps = 1/311 (0%)
 Frame = +2

Query: 2    VFSKDVFVFDDEDRIG-MQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKV 178
            + S+D   F+D+ + G M+ L+++++M  A+PNL +FY I  GLDLQGL ++  + + ++
Sbjct: 633  LMSRDFISFEDDYKDGGMKGLVRKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARI 692

Query: 179  NKLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETSS 358
              +WE  ++                  +D L++L+ + + D QIN LF+E    G++TSS
Sbjct: 693  CSMWESVIRERREGASDDPSK------QDFLNILIRSGYSDDQINQLFMELLTAGADTSS 746

Query: 359  ATIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAP 538
            +T+EWAMAELIK+P  + KV +EL + +      K + +PHLPYLQAC+KET+RLHP+AP
Sbjct: 747  STLEWAMAELIKSPESMKKVHEELAREISDNLP-KASDLPHLPYLQACVKETLRLHPSAP 805

Query: 539  FLLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNH 718
             LLP RA  +C+VM YT+PKD +I VNA+AIGRDP  W DP  F PERFL S VD+ GN+
Sbjct: 806  LLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNN 865

Query: 719  FEFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAID 898
             EFIPFG+GRRIC GLP+A R +PLI+ASL H F WSLP+   P+EL MN+   ++L  +
Sbjct: 866  LEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLPNGTTPDELDMNDKFGIALQKE 925

Query: 899  PSLSIIPKVRE 931
              L IIPKVR+
Sbjct: 926  QPLLIIPKVRK 936


>ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
            2-like [Vitis vinifera]
          Length = 503

 Score =  303 bits (777), Expect = 7e-80
 Identities = 150/311 (48%), Positives = 211/311 (67%), Gaps = 1/311 (0%)
 Frame = +2

Query: 2    VFSKDVFVFDDEDRIG-MQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKV 178
            + S+D   F+D+ + G M+ L+++++M  A+PNL +FY I  GLDLQGL ++  + + ++
Sbjct: 200  LMSRDFISFEDDYKDGGMKGLVRKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARI 259

Query: 179  NKLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETSS 358
              +WE  ++                  +D L++L+ + + D QIN LF+E    G++TSS
Sbjct: 260  CSMWESVIRERREGASDDPSK------QDFLNILIRSGYSDDQINQLFMELLTAGADTSS 313

Query: 359  ATIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAP 538
            +T+EWAMAELIK+P  + KV +EL + +      K + +PHLPYLQAC+KET+RLHP+AP
Sbjct: 314  STLEWAMAELIKSPESMKKVHEELAREISDNLP-KASDLPHLPYLQACVKETLRLHPSAP 372

Query: 539  FLLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNH 718
             LLP RA  +C+VM YT+PKD +I VNA+AIGRDP  W DP  F PERFL S VD+ GN+
Sbjct: 373  LLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNN 432

Query: 719  FEFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAID 898
             EFIPFG+GRRIC GLP+A R +PLI+ASL H F WSLP+   P+EL MN+   ++L  +
Sbjct: 433  LEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLPNGTTPDELDMNDKFGIALQKE 492

Query: 899  PSLSIIPKVRE 931
              L IIPKVR+
Sbjct: 493  QPLLIIPKVRK 503


>emb|CBI34715.3| unnamed protein product [Vitis vinifera]
          Length = 1076

 Score =  302 bits (773), Expect = 2e-79
 Identities = 149/311 (47%), Positives = 211/311 (67%), Gaps = 1/311 (0%)
 Frame = +2

Query: 2    VFSKDVFVFDDEDRIG-MQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKV 178
            + S+D   F+D+++ G M+ L+++++M  A+PNL +FY I  GLDLQGL ++  + + ++
Sbjct: 764  LMSRDFISFEDDNKDGGMKGLVRKMVMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARI 823

Query: 179  NKLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETSS 358
              +WE  ++                  +D L++L+ + + D QIN LF+E    G++TSS
Sbjct: 824  CSMWESVIRERREGASDDPSK------QDFLNILIRSGYSDDQINQLFMELLTAGADTSS 877

Query: 359  ATIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAP 538
            +T+EWAMAELIK+P  + KV +EL + +      K + +PHLPYLQAC+KET+RLHP+AP
Sbjct: 878  STLEWAMAELIKSPESMKKVHEELAREISDNLP-KASDLPHLPYLQACVKETLRLHPSAP 936

Query: 539  FLLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNH 718
             LLP RA  +C+VM YT+PKD +I VNA+AIGRDP  W DP  F PERFL S VD+ GN+
Sbjct: 937  LLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNN 996

Query: 719  FEFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAID 898
             EFIPFG+GRRIC GLP+A R +PLI+ASL H F WSLP+   P+EL MN+   ++L  +
Sbjct: 997  LEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLPNGTTPDELDMNDKFGVTLQKE 1056

Query: 899  PSLSIIPKVRE 931
              L IIPK R+
Sbjct: 1057 QPLLIIPKKRK 1067


>ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
            communis] gi|223547255|gb|EEF48750.1|
            (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
            [Ricinus communis]
          Length = 496

 Score =  301 bits (770), Expect = 4e-79
 Identities = 148/308 (48%), Positives = 208/308 (67%), Gaps = 1/308 (0%)
 Frame = +2

Query: 2    VFSKDVFVFDDEDRIG-MQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKV 178
            +FSKD+  F+D+   G +++LI  L+ +   PN+A+FYP+L  LD QG+KR+  + + ++
Sbjct: 193  LFSKDLLSFEDQGNAGELKTLISTLMELATCPNIADFYPVLTKLDPQGIKRKMKNCLERM 252

Query: 179  NKLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETSS 358
              +W+  +K                   D LDV L N F D QIN L LE F  G++T++
Sbjct: 253  FGVWDIYIKERRERHVKDARKT------DFLDVFLSNGFDDHQINWLLLELFSAGADTTT 306

Query: 359  ATIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAP 538
             T+EWAMAEL+K  T L KVR+EL+  +G K  ++E+HIP L YL AC+KET+RLHP  P
Sbjct: 307  TTVEWAMAELLKEITVLEKVREELETEIG-KDMIRESHIPQLKYLNACVKETLRLHPPVP 365

Query: 539  FLLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNH 718
            FL+P RA E C+VM YT+PK  +IIVN +AIGRDP+ W DP ++ PERFL+SN+D+ G++
Sbjct: 366  FLIPRRAPEACEVMNYTIPKHSQIIVNVWAIGRDPSAWEDPLSYRPERFLDSNLDFKGHN 425

Query: 719  FEFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAID 898
            FEF+PFGSGRRIC GLP+  R +PLI+ASL+H F WSL +   P  L MN+  S++L  +
Sbjct: 426  FEFLPFGSGRRICPGLPMGTRQLPLILASLVHCFDWSLQNGDDPAMLDMNDKFSITLEKE 485

Query: 899  PSLSIIPK 922
              L ++PK
Sbjct: 486  QHLLVVPK 493


>ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
            communis] gi|223549945|gb|EEF51432.1|
            (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
            [Ricinus communis]
          Length = 496

 Score =  294 bits (753), Expect = 4e-77
 Identities = 147/307 (47%), Positives = 207/307 (67%)
 Frame = +2

Query: 2    VFSKDVFVFDDEDRIGMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVN 181
            +FS D+  F+++   G++SL+  ++ +  SPN+AEFYPIL  LD QGLKR+    + ++ 
Sbjct: 192  LFSTDLIGFENQIG-GVKSLLWSMMEMATSPNIAEFYPILAPLDPQGLKRKMTKCLKEMF 250

Query: 182  KLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETSSA 361
             +WE  +K                   D LD+ L N F D QIN L +E    G++T+S 
Sbjct: 251  GVWEIYIKERRRTHDHDHAAPKT----DFLDIFLSNGFDDDQINWLVMELLSAGTDTTST 306

Query: 362  TIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPF 541
            ++EWAMAEL+KN   + KVR+ELD+ +  K+ +KE+H+  LPYL AC+KET+RLHP APF
Sbjct: 307  SVEWAMAELLKNKEAMKKVREELDREIN-KNPIKESHVSQLPYLNACVKETLRLHPPAPF 365

Query: 542  LLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHF 721
            L+P RA E C+VM YT+PKD +++VN +AIGRDP+ W DPS+F PERFL S++D  G+ F
Sbjct: 366  LIPRRATENCEVMNYTIPKDSQVLVNVWAIGRDPSVWEDPSSFKPERFLGSSLDVKGHDF 425

Query: 722  EFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAIDP 901
            E IPFGSGRRIC GLP+A R + L++ASL+H F WSLP+   P ++ M E   ++L ++ 
Sbjct: 426  ELIPFGSGRRICPGLPMATRQLSLVLASLIHCFDWSLPNGGDPAKVDMTEKFGITLQMEH 485

Query: 902  SLSIIPK 922
             L IIPK
Sbjct: 486  PLLIIPK 492


>gb|EXC34114.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 [Morus notabilis]
          Length = 515

 Score =  293 bits (750), Expect = 9e-77
 Identities = 139/295 (47%), Positives = 198/295 (67%)
 Frame = +2

Query: 47   GMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKVNKLWEPTVKXXXXXXX 226
            G+ +  +EL  +G  PN+++ YP+L  LDLQ L+++  +  GKV +LW+  VK       
Sbjct: 221  GICAFWRELFGLGGIPNISDMYPMLGPLDLQKLRKKSMEMFGKVCELWDDIVKERKKMRK 280

Query: 227  XXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETSSATIEWAMAELIKNPTK 406
                        D LD +L N + D QIN+L LE F  G+++SS T+EW MAEL++NP  
Sbjct: 281  SYYKDASPRPQLDFLDAMLENGYPDFQINILLLELFSAGTDSSSTTVEWLMAELVRNPKC 340

Query: 407  LSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAPFLLPHRAEETCQVMGY 586
            +  +R+EL++ +G +  V E  +P L YL+ C+KE +RL P AP  +PHRA ETCQVM Y
Sbjct: 341  MKILREELEREIGSQEMVMECDLPKLKYLECCLKEPLRLPPPAPLPIPHRAAETCQVMNY 400

Query: 587  TVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNHFEFIPFGSGRRICVGL 766
            TVPK+ +I+VN +AIGRDPN W DP  F PERFL S++D+ G +FE++PFG+GRRIC G 
Sbjct: 401  TVPKNSEILVNVWAIGRDPNHWEDPLEFKPERFLNSSLDFKGTNFEYLPFGAGRRICPGQ 460

Query: 767  PLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAIDPSLSIIPKVRE 931
            PLA++ +PLIVASL+H+F WSLP+ K P+EL M E   +++     L ++PKVR+
Sbjct: 461  PLASKQVPLIVASLVHSFDWSLPNGKDPKELDMAEKYDINMLKKEPLLVVPKVRK 515


>ref|XP_003631391.1| PREDICTED: LOW QUALITY PROTEIN: (S)-N-methylcoclaurine 3'-hydroxylase
            isozyme 1-like [Vitis vinifera]
          Length = 497

 Score =  290 bits (742), Expect = 8e-76
 Identities = 145/311 (46%), Positives = 209/311 (67%), Gaps = 1/311 (0%)
 Frame = +2

Query: 2    VFSKDVFVFDDEDRIGMQS-LIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKV 178
            + SKD+  F+DE  + M   + K +L + ++PNL+++YPIL GLDLQ L++R      K 
Sbjct: 193  LLSKDLVTFEDEKALAMMGEIFKTILEVTSTPNLSDYYPILRGLDLQRLQKRSIISFVKF 252

Query: 179  NKLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETSS 358
              + +P +K                  +D LD L+ + F D QIN+L +E    G+++SS
Sbjct: 253  CSILKPIIKERRERKGGHATSQ-----QDFLDTLISDGFTDDQINILLVELLVAGTDSSS 307

Query: 359  ATIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAP 538
             T+EWAMAELI++P  L K+R+EL   +  ++ +K++ +  LPYLQAC+KET+RLHP  P
Sbjct: 308  VTVEWAMAELIRSPESLKKIREELTTEIN-QNMLKDSDLRKLPYLQACLKETLRLHPPGP 366

Query: 539  FLLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNH 718
            FLLPHRA E+C+VM YT+PKD +++VNA+AIGRDP +W DP  F PERFL S VD+ GN+
Sbjct: 367  FLLPHRAVESCKVMNYTIPKDAQVLVNAWAIGRDPMSWEDPLVFKPERFLNSTVDFQGNN 426

Query: 719  FEFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAID 898
            FEFIPF S RRIC GLP+A + IPL++AS +H F WSLP+   P+++ M+E  S ++  +
Sbjct: 427  FEFIPFSSRRRICPGLPMAVKLIPLVLASWIHFFDWSLPNGGDPKDIDMSEKYSANIRKE 486

Query: 899  PSLSIIPKVRE 931
              L +IPK R+
Sbjct: 487  QPLLLIPKGRK 497


>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
          Length = 488

 Score =  290 bits (742), Expect = 8e-76
 Identities = 160/313 (51%), Positives = 202/313 (64%), Gaps = 4/313 (1%)
 Frame = +2

Query: 2    VFSKDVFVFDDEDRIGMQSLIKE----LLMIGASPNLAEFYPILEGLDLQGLKRRCADRV 169
            +FSKDVF  D ED  G    +KE    LL +G S N A+++PI+   DL G +R  AD +
Sbjct: 182  IFSKDVF--DLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVL 239

Query: 170  GKVNKLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSE 349
             ++  +W   +K                   D +DVLL+    D +IN L +E FG G+E
Sbjct: 240  QQIYDVWGINLKERRGMKGSESN-------NDFVDVLLNAGLEDQKINALLMELFGTGTE 292

Query: 350  TSSATIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHP 529
            TS++TIEWA+ EL K P  +SK+R EL  VVG  + VKE+ +PHLPYLQA +KET+RLHP
Sbjct: 293  TSASTIEWAITELTKKPLVVSKIRLELVNVVGDNT-VKESDLPHLPYLQAFVKETLRLHP 351

Query: 530  AAPFLLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYN 709
              P LLP RA ETC VM YT+PK+ +I+VNA+AIGRDP TW DP  F PERFL S+VDY 
Sbjct: 352  PTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYK 411

Query: 710  GNHFEFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSL 889
            GN FE IPFG GRRIC GLPLA++ I LIVA+L+ NF WSLP      EL+M+E   L+L
Sbjct: 412  GNDFELIPFGGGRRICPGLPLASQFISLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTL 471

Query: 890  AIDPSLSIIPKVR 928
              DP L I+ K R
Sbjct: 472  QKDPPLLIVLKAR 484


>ref|XP_002274586.2| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2-like
            [Vitis vinifera]
          Length = 498

 Score =  289 bits (739), Expect = 2e-75
 Identities = 145/312 (46%), Positives = 204/312 (65%), Gaps = 2/312 (0%)
 Frame = +2

Query: 5    FSKDVFVFDDEDRIG--MQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKV 178
            FS D   F+  D  G  ++ L+ E   +GA+ N+ +FYPIL GLD+QG++++  +   ++
Sbjct: 195  FSIDFSDFEGRDVRGVLLKDLLNENAELGAT-NILDFYPILGGLDIQGIRKKLKEIFRRI 253

Query: 179  NKLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETSS 358
               WE  +K                   D LD LL   F D QIN + +E F  G ETSS
Sbjct: 254  PTTWEDILKERRKQRIHGSSHG------DFLDALLETGFEDDQINHVIMELFFAGPETSS 307

Query: 359  ATIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAP 538
             T+EWAMAELIKN   + K+  EL Q++G +S V+E+H+PHLPYLQAC+KET+RLHP  P
Sbjct: 308  LTVEWAMAELIKNQDAMHKLCNELTQIIG-ESPVRESHLPHLPYLQACVKETLRLHPTGP 366

Query: 539  FLLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNH 718
             LLPHRA ETCQ+MGYT+PKD  I VN +A+GRDP TW DP +F PERFL+S +++ GN 
Sbjct: 367  LLLPHRATETCQIMGYTIPKDSIIFVNMWAMGRDPGTWEDPLSFKPERFLDSKLEFKGND 426

Query: 719  FEFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAID 898
            FE+IPFG+GRR+C G+PLA R +P+I+A+ +  F WS P +    E+ M E   ++L  +
Sbjct: 427  FEYIPFGAGRRMCPGMPLAARLVPMILATFVRLFDWSTPGDMDFAEIDMEERFVITLRKE 486

Query: 899  PSLSIIPKVREH 934
              L ++P++R++
Sbjct: 487  QPLRLVPRIRKY 498


>gb|EXC34118.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Morus notabilis]
          Length = 499

 Score =  288 bits (737), Expect = 3e-75
 Identities = 142/310 (45%), Positives = 205/310 (66%), Gaps = 1/310 (0%)
 Frame = +2

Query: 2    VFSKDVFVFDDE-DRIGMQSLIKELLMIGASPNLAEFYPILEGLDLQGLKRRCADRVGKV 178
            + S+D+  F+ E D  GM  +++ ++ +   PN+++FYP L   DLQGL+++  +   K+
Sbjct: 194  LLSRDLINFEQESDDGGMSGVLRTIMEVAIQPNISDFYPFLGPFDLQGLRKKSMEIYHKL 253

Query: 179  NKLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSETSS 358
              ++E   K                  +D LD L+     + QIN L +E    G++TSS
Sbjct: 254  CAIFEAITKERREKKSGDIPSQ-----QDFLDALISIGSSNDQINFLLIELITAGTDTSS 308

Query: 359  ATIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHPAAP 538
            +TIEW + ELIKNP  +  V+ EL + +  +  V+E+H+P LPYLQAC+KET+RLHP AP
Sbjct: 309  STIEWTLTELIKNPKCMKIVKAELAREIVDQDMVRESHLPKLPYLQACVKETLRLHPPAP 368

Query: 539  FLLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYNGNH 718
            FLLPHRA E+CQV+ YT+PKD +++VN +AIGRDP  W DP TF PERFLES++D+ GN 
Sbjct: 369  FLLPHRAVESCQVLNYTIPKDSQLLVNVWAIGRDPMYWKDPLTFKPERFLESSLDFKGND 428

Query: 719  FEFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSLAID 898
            FEF+PFG+GRRIC GLP+A + +PL+VASL+H   WSLP  K P ++ M+E   ++L  +
Sbjct: 429  FEFLPFGAGRRICPGLPMAAKHVPLVVASLIHFLDWSLPYGKDPLDMNMSEKYGITLIKE 488

Query: 899  PSLSIIPKVR 928
              L +IPKV+
Sbjct: 489  EPLILIPKVK 498


>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
            2; AltName: Full=Cytochrome P450 80B2
            gi|9971208|dbj|BAB12433.1|
            (S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
          Length = 488

 Score =  288 bits (736), Expect = 4e-75
 Identities = 159/313 (50%), Positives = 202/313 (64%), Gaps = 4/313 (1%)
 Frame = +2

Query: 2    VFSKDVFVFDDEDRIGMQSLIKE----LLMIGASPNLAEFYPILEGLDLQGLKRRCADRV 169
            +FSKDVF  D ED  G    +KE    LL +G S N A+++PI+  LDL G +R  A+ +
Sbjct: 182  IFSKDVF--DLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVL 239

Query: 170  GKVNKLWEPTVKXXXXXXXXXXXXXXXXXXKDMLDVLLHNEFGDVQINVLFLETFGPGSE 349
             ++  +W   +K                   D +DVLL+    D +IN L +E FG G+E
Sbjct: 240  QQIYDVWGVMLKERRGTKGSESK-------NDFVDVLLNAGLDDQKINALLMELFGAGTE 292

Query: 350  TSSATIEWAMAELIKNPTKLSKVRQELDQVVGPKSQVKEAHIPHLPYLQACIKETMRLHP 529
            TS++TIEWA+ EL K P  +SK+R EL  VVG  + VKE+ +PHLPYLQA +KET+RLHP
Sbjct: 293  TSASTIEWAITELTKKPLVVSKIRLELVNVVGDNT-VKESDLPHLPYLQAFVKETLRLHP 351

Query: 530  AAPFLLPHRAEETCQVMGYTVPKDYKIIVNAYAIGRDPNTWTDPSTFWPERFLESNVDYN 709
              P LLP RA ETC VM YT+PK+ +I+VNA+AIGRDP TW DP  F PERFL S+VDY 
Sbjct: 352  PTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYK 411

Query: 710  GNHFEFIPFGSGRRICVGLPLANRTIPLIVASLLHNFAWSLPDEKRPEELAMNEILSLSL 889
            GN FE IPFG GRRIC GLPLA++   LIVA+L+ NF WSLP      EL+M+E   L+L
Sbjct: 412  GNDFELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTL 471

Query: 890  AIDPSLSIIPKVR 928
              DP L I+ K R
Sbjct: 472  QKDPPLLIVLKAR 484