BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cocculus23_contig00001922
         (524 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002279634.2| PREDICTED: probable receptor-like protein ki...   112   4e-23
emb|CBI26984.3| unnamed protein product [Vitis vinifera]              112   4e-23
gb|EXB37547.1| putative receptor-like protein kinase [Morus nota...   104   1e-20
ref|XP_007142939.1| hypothetical protein PHAVU_007G030000g [Phas...   104   1e-20
gb|EXB88612.1| putative receptor-like protein kinase [Morus nota...   103   2e-20
gb|EXB37546.1| putative receptor-like protein kinase [Morus nota...   103   2e-20
ref|XP_006485858.1| PREDICTED: probable receptor-like protein ki...   103   2e-20
ref|XP_002280726.1| PREDICTED: probable receptor-like protein ki...   103   2e-20
emb|CAN76987.1| hypothetical protein VITISV_027948 [Vitis vinifera]   103   3e-20
gb|EYU40780.1| hypothetical protein MIMGU_mgv1a005995mg [Mimulus...   102   4e-20
ref|XP_006468744.1| PREDICTED: probable receptor-like protein ki...   102   4e-20
ref|XP_006448424.1| hypothetical protein CICLE_v10014567mg [Citr...   102   4e-20
ref|XP_002279790.2| PREDICTED: probable receptor-like protein ki...   102   4e-20
ref|XP_006482224.1| PREDICTED: probable receptor-like protein ki...   102   6e-20
gb|EXB88614.1| putative receptor-like protein kinase [Morus nota...   102   7e-20
ref|XP_002527018.1| receptor serine/threonine kinase, putative [...   102   7e-20
ref|XP_006605917.1| PREDICTED: probable receptor-like protein ki...   101   9e-20
ref|XP_006383424.1| hypothetical protein POPTR_0005s15370g [Popu...   101   9e-20
ref|XP_006442612.1| hypothetical protein CICLE_v10021200mg [Citr...   101   1e-19
ref|XP_006430738.1| hypothetical protein CICLE_v10013644mg [Citr...   101   1e-19

>ref|XP_002279634.2| PREDICTED: probable receptor-like protein kinase At1g67000-like
           [Vitis vinifera]
          Length = 459

 Score =  112 bits (281), Expect = 4e-23
 Identities = 55/89 (61%), Positives = 69/89 (77%)
 Frame = -3

Query: 267 WSRKRDLKRTKNAHLFEDCLQNHPSLTIKRYSFSDIKKMTKSFHDKLGQGGYGAVFKGKL 88
           W   + +KRTKN    E  L+NH SL +KRY  SD+KKMT SF DK+G+GGYG V+KGKL
Sbjct: 115 WRNCKSIKRTKNQPNIEAFLRNHGSLALKRYRHSDVKKMTNSFKDKIGEGGYGDVYKGKL 174

Query: 87  LDDGRLVVVKLLTESQGNGQEFINEVASI 1
           L DG++V VK+L E++GNG+EFINEVASI
Sbjct: 175 L-DGQMVAVKVLNETKGNGEEFINEVASI 202


>emb|CBI26984.3| unnamed protein product [Vitis vinifera]
          Length = 621

 Score =  112 bits (281), Expect = 4e-23
 Identities = 55/89 (61%), Positives = 69/89 (77%)
 Frame = -3

Query: 267 WSRKRDLKRTKNAHLFEDCLQNHPSLTIKRYSFSDIKKMTKSFHDKLGQGGYGAVFKGKL 88
           W   + +KRTKN    E  L+NH SL +KRY  SD+KKMT SF DK+G+GGYG V+KGKL
Sbjct: 277 WRNCKSIKRTKNQPNIEAFLRNHGSLALKRYRHSDVKKMTNSFKDKIGEGGYGDVYKGKL 336

Query: 87  LDDGRLVVVKLLTESQGNGQEFINEVASI 1
           L DG++V VK+L E++GNG+EFINEVASI
Sbjct: 337 L-DGQMVAVKVLNETKGNGEEFINEVASI 364


>gb|EXB37547.1| putative receptor-like protein kinase [Morus notabilis]
          Length = 644

 Score =  104 bits (260), Expect = 1e-20
 Identities = 50/79 (63%), Positives = 64/79 (81%)
 Frame = -3

Query: 237 KNAHLFEDCLQNHPSLTIKRYSFSDIKKMTKSFHDKLGQGGYGAVFKGKLLDDGRLVVVK 58
           KN    ED ++++  L ++RY+FSD+KKMTKSF +KLGQGGYG V+KGKLLD  RLV VK
Sbjct: 296 KNDQGLEDFIRSYAYLNVQRYAFSDLKKMTKSFKEKLGQGGYGEVYKGKLLDGERLVAVK 355

Query: 57  LLTESQGNGQEFINEVASI 1
           +L  S+GNG++FINEVASI
Sbjct: 356 VLNASKGNGEDFINEVASI 374


>ref|XP_007142939.1| hypothetical protein PHAVU_007G030000g [Phaseolus vulgaris]
           gi|561016129|gb|ESW14933.1| hypothetical protein
           PHAVU_007G030000g [Phaseolus vulgaris]
          Length = 620

 Score =  104 bits (260), Expect = 1e-20
 Identities = 64/164 (39%), Positives = 81/164 (49%)
 Frame = -3

Query: 492 NCSECQQVRGGSCLEDANGNFQCGSLKNNTVNNTSSDYGAGHGNARRRKAFIIAXXXXXX 313
           NC  C    GG C  D  G F C                   GN   +   I+       
Sbjct: 210 NCFRCLYANGGQCRVDNTGMFYCDQ-----------------GNKSTKIRLIVTTVACVG 252

Query: 312 XXXXXXXXXXXXLQCWSRKRDLKRTKNAHLFEDCLQNHPSLTIKRYSFSDIKKMTKSFHD 133
                          + R+R  K+     + E  L  H  L  KRYS+S+IKK T SF +
Sbjct: 253 GTLGVLIFLGW----FIRRRFYKKNPTYEIIEMFLNTHGHLAAKRYSYSEIKKATNSFRN 308

Query: 132 KLGQGGYGAVFKGKLLDDGRLVVVKLLTESQGNGQEFINEVASI 1
           KLG+GGYG+V+KGK L+DG LV VK+L+ES+GNG+EFINEVASI
Sbjct: 309 KLGEGGYGSVYKGK-LNDGSLVAVKVLSESKGNGEEFINEVASI 351


>gb|EXB88612.1| putative receptor-like protein kinase [Morus notabilis]
          Length = 651

 Score =  103 bits (257), Expect = 2e-20
 Identities = 67/172 (38%), Positives = 90/172 (52%), Gaps = 1/172 (0%)
 Frame = -3

Query: 513 LRWVVLPNCSECQQVRGGSCLEDANGNFQCGSLKNNTVNNTSSDYGAGHGNARRRKAFII 334
           + ++  P CSEC+   G     ++NG   C              Y   H + R  K    
Sbjct: 220 VNYMEFPLCSECEWSSGRCGSNNSNGMVTC-----YCSGGREYLYVCPHSSGRDWKLKTG 274

Query: 333 AXXXXXXXXXXXXXXXXXXLQCWSRKRDL-KRTKNAHLFEDCLQNHPSLTIKRYSFSDIK 157
                               +C   K+ L K+T+N    E  ++++  LT KRYSF +++
Sbjct: 275 IGIVSGGSALLLIGIITCWCRCSKSKKSLTKKTQNDQEPEAFIRSYGHLTAKRYSFFEVE 334

Query: 156 KMTKSFHDKLGQGGYGAVFKGKLLDDGRLVVVKLLTESQGNGQEFINEVASI 1
           KMTKSF DKLGQGG+G V+KGKLL DGR V VK+L  S+GNG+EFINEVASI
Sbjct: 335 KMTKSFKDKLGQGGFGEVYKGKLL-DGRHVAVKILNASKGNGEEFINEVASI 385


>gb|EXB37546.1| putative receptor-like protein kinase [Morus notabilis]
          Length = 655

 Score =  103 bits (257), Expect = 2e-20
 Identities = 49/79 (62%), Positives = 64/79 (81%)
 Frame = -3

Query: 237 KNAHLFEDCLQNHPSLTIKRYSFSDIKKMTKSFHDKLGQGGYGAVFKGKLLDDGRLVVVK 58
           KN    ED ++++  L ++RY+FSD+KKMTKSF +KLGQGGYG V++GKLLD  RLV VK
Sbjct: 308 KNDQDLEDFIRSYAYLNVQRYAFSDLKKMTKSFKEKLGQGGYGEVYRGKLLDGERLVAVK 367

Query: 57  LLTESQGNGQEFINEVASI 1
           +L  S+GNG++FINEVASI
Sbjct: 368 VLNASKGNGEDFINEVASI 386


>ref|XP_006485858.1| PREDICTED: probable receptor-like protein kinase At1g67000-like
           [Citrus sinensis]
          Length = 793

 Score =  103 bits (257), Expect = 2e-20
 Identities = 73/173 (42%), Positives = 87/173 (50%), Gaps = 10/173 (5%)
 Frame = -3

Query: 489 CSECQQVRGGSCLEDANGNFQCGSLKNNTVNNTSSDYGAGHGNARRRKAFIIAXXXXXXX 310
           CS C   + G           CGS  N + +  S  Y   H N  R   F IA       
Sbjct: 378 CSTCTSSQSGGI---------CGS--NQSSDEFSCLYPPRHDNGSRTSRFGIALGAGMMG 426

Query: 309 XXXXXXXXXXXLQCWSRKRD----------LKRTKNAHLFEDCLQNHPSLTIKRYSFSDI 160
                        C  RK++           K TK+    E  ++N+ SL  KRYSF ++
Sbjct: 427 IIITCAII-----CIFRKKNNSSILWTICCRKVTKSDQDLEAIIRNYGSLAPKRYSFPNV 481

Query: 159 KKMTKSFHDKLGQGGYGAVFKGKLLDDGRLVVVKLLTESQGNGQEFINEVASI 1
           KKMT SF D+LGQGGYG V+KGK L DGRLV VKLL  S+GNGQEFINEVASI
Sbjct: 482 KKMTNSFKDELGQGGYGRVYKGK-LSDGRLVAVKLLNTSKGNGQEFINEVASI 533


>ref|XP_002280726.1| PREDICTED: probable receptor-like protein kinase At1g67000-like
           [Vitis vinifera]
          Length = 545

 Score =  103 bits (257), Expect = 2e-20
 Identities = 52/82 (63%), Positives = 65/82 (79%)
 Frame = -3

Query: 246 KRTKNAHLFEDCLQNHPSLTIKRYSFSDIKKMTKSFHDKLGQGGYGAVFKGKLLDDGRLV 67
           K+T+N+   E  L+ + SL  KRYS+SD+KKMT SF +KLGQGGYG V+KG  L DG LV
Sbjct: 190 KKTRNSQNVEAFLEKYASLAPKRYSYSDVKKMTDSFRNKLGQGGYGIVYKGN-LPDGHLV 248

Query: 66  VVKLLTESQGNGQEFINEVASI 1
           VVK+L+E +GNG+EFINEVASI
Sbjct: 249 VVKILSEPKGNGEEFINEVASI 270


>emb|CAN76987.1| hypothetical protein VITISV_027948 [Vitis vinifera]
          Length = 620

 Score =  103 bits (256), Expect = 3e-20
 Identities = 51/82 (62%), Positives = 66/82 (80%)
 Frame = -3

Query: 246 KRTKNAHLFEDCLQNHPSLTIKRYSFSDIKKMTKSFHDKLGQGGYGAVFKGKLLDDGRLV 67
           K+T N    E  L+NH SL  KRY +SD+KKMT SF DKLG+GGYG V+KGK++ DG++V
Sbjct: 284 KQTGNHPNVEVFLRNHESLAPKRYRYSDVKKMTNSFKDKLGEGGYGGVYKGKMV-DGQMV 342

Query: 66  VVKLLTESQGNGQEFINEVASI 1
            VK+L+E++GNG+EFINEVASI
Sbjct: 343 AVKVLSETKGNGEEFINEVASI 364


>gb|EYU40780.1| hypothetical protein MIMGU_mgv1a005995mg [Mimulus guttatus]
          Length = 461

 Score =  102 bits (255), Expect = 4e-20
 Identities = 51/79 (64%), Positives = 64/79 (81%)
 Frame = -3

Query: 237 KNAHLFEDCLQNHPSLTIKRYSFSDIKKMTKSFHDKLGQGGYGAVFKGKLLDDGRLVVVK 58
           K+  LF   L+N+ +L  KRY +SDIKKMTKSF + LG+GGYG+V+KGK+ DDGRLV VK
Sbjct: 126 KDIELF---LKNNGNLAPKRYKYSDIKKMTKSFSESLGKGGYGSVYKGKVSDDGRLVAVK 182

Query: 57  LLTESQGNGQEFINEVASI 1
           +L ES+GNG EF+NEVASI
Sbjct: 183 MLNESKGNGDEFMNEVASI 201


>ref|XP_006468744.1| PREDICTED: probable receptor-like protein kinase At1g67000-like
           [Citrus sinensis]
          Length = 660

 Score =  102 bits (255), Expect = 4e-20
 Identities = 52/82 (63%), Positives = 65/82 (79%)
 Frame = -3

Query: 246 KRTKNAHLFEDCLQNHPSLTIKRYSFSDIKKMTKSFHDKLGQGGYGAVFKGKLLDDGRLV 67
           K+T N    E  L+N+ SL  KRYS++DIKKMT SF +KLGQGGYG V+KGKLL DGR V
Sbjct: 314 KKTDNHRNIEAFLRNYGSLAPKRYSYADIKKMTNSFKNKLGQGGYGGVYKGKLL-DGRNV 372

Query: 66  VVKLLTESQGNGQEFINEVASI 1
            +K+L E++GNG++FINEVASI
Sbjct: 373 AIKVLNETKGNGEDFINEVASI 394


>ref|XP_006448424.1| hypothetical protein CICLE_v10014567mg [Citrus clementina]
           gi|557551035|gb|ESR61664.1| hypothetical protein
           CICLE_v10014567mg [Citrus clementina]
          Length = 644

 Score =  102 bits (255), Expect = 4e-20
 Identities = 52/82 (63%), Positives = 65/82 (79%)
 Frame = -3

Query: 246 KRTKNAHLFEDCLQNHPSLTIKRYSFSDIKKMTKSFHDKLGQGGYGAVFKGKLLDDGRLV 67
           K+T N    E  L+N+ SL  KRYS++DIKKMT SF +KLGQGGYG V+KGKLL DGR V
Sbjct: 298 KKTDNHRNIEAFLRNYGSLAPKRYSYADIKKMTNSFKNKLGQGGYGGVYKGKLL-DGRNV 356

Query: 66  VVKLLTESQGNGQEFINEVASI 1
            +K+L E++GNG++FINEVASI
Sbjct: 357 AIKVLNETKGNGEDFINEVASI 378


>ref|XP_002279790.2| PREDICTED: probable receptor-like protein kinase At1g67000-like
           [Vitis vinifera]
          Length = 681

 Score =  102 bits (255), Expect = 4e-20
 Identities = 51/86 (59%), Positives = 67/86 (77%)
 Frame = -3

Query: 258 KRDLKRTKNAHLFEDCLQNHPSLTIKRYSFSDIKKMTKSFHDKLGQGGYGAVFKGKLLDD 79
           K+ +    N  +F   L+NH SL  KRY +SD+KKMT SF DKLG+GGYG V+KGK+L D
Sbjct: 344 KKQMGNHPNVEVF---LRNHESLAPKRYRYSDVKKMTNSFKDKLGEGGYGGVYKGKML-D 399

Query: 78  GRLVVVKLLTESQGNGQEFINEVASI 1
           G++V VK+L+E++GNG+EFINEVASI
Sbjct: 400 GQMVAVKVLSETKGNGEEFINEVASI 425


>ref|XP_006482224.1| PREDICTED: probable receptor-like protein kinase At1g67000-like
           [Citrus sinensis]
          Length = 550

 Score =  102 bits (254), Expect = 6e-20
 Identities = 65/163 (39%), Positives = 88/163 (53%)
 Frame = -3

Query: 489 CSECQQVRGGSCLEDANGNFQCGSLKNNTVNNTSSDYGAGHGNARRRKAFIIAXXXXXXX 310
           C EC+Q  G        G F C     N   ++S     G    ++RK ++         
Sbjct: 155 CEECKQSNGNCGYIAKTGEFICYC--PNGAYSSSCHSRTGSFLNKKRKIYV-GVAASVLG 211

Query: 309 XXXXXXXXXXXLQCWSRKRDLKRTKNAHLFEDCLQNHPSLTIKRYSFSDIKKMTKSFHDK 130
                      +Q   +K+  +  +N   F   ++NH SL  KRYS+SD+K+MTKSF DK
Sbjct: 212 TMVILGILLIVIQALIKKKTREDDRNVEAF---IRNHESLAPKRYSYSDVKRMTKSFRDK 268

Query: 129 LGQGGYGAVFKGKLLDDGRLVVVKLLTESQGNGQEFINEVASI 1
           LGQGGYG V+KG+ L DG+LV VK+L  S+ NG+EFINEVASI
Sbjct: 269 LGQGGYGEVYKGE-LPDGQLVAVKVLKNSRCNGEEFINEVASI 310


>gb|EXB88614.1| putative receptor-like protein kinase [Morus notabilis]
          Length = 644

 Score =  102 bits (253), Expect = 7e-20
 Identities = 48/79 (60%), Positives = 64/79 (81%)
 Frame = -3

Query: 237 KNAHLFEDCLQNHPSLTIKRYSFSDIKKMTKSFHDKLGQGGYGAVFKGKLLDDGRLVVVK 58
           KN    E+ ++++  L ++RY+FSD+KKMTKSF +KLGQGGYG V++GKLLD  RLV VK
Sbjct: 296 KNDRDLEEFIRSYAYLNVQRYAFSDLKKMTKSFKEKLGQGGYGEVYRGKLLDGERLVAVK 355

Query: 57  LLTESQGNGQEFINEVASI 1
           +L  S+GNG++FINEVASI
Sbjct: 356 VLNASKGNGEDFINEVASI 374


>ref|XP_002527018.1| receptor serine/threonine kinase, putative [Ricinus communis]
           gi|223533653|gb|EEF35390.1| receptor serine/threonine
           kinase, putative [Ricinus communis]
          Length = 435

 Score =  102 bits (253), Expect = 7e-20
 Identities = 52/90 (57%), Positives = 66/90 (73%)
 Frame = -3

Query: 270 CWSRKRDLKRTKNAHLFEDCLQNHPSLTIKRYSFSDIKKMTKSFHDKLGQGGYGAVFKGK 91
           CW      K+T+ +   E  L+N   + +KRY ++++KKMT+SF DKLGQGGYG VFKGK
Sbjct: 57  CWK-----KQTEESKTIEAFLRNDGPMAMKRYKYTEVKKMTQSFKDKLGQGGYGGVFKGK 111

Query: 90  LLDDGRLVVVKLLTESQGNGQEFINEVASI 1
            L DGR V VK+L ES+GNG+EFINEVASI
Sbjct: 112 -LPDGRDVAVKILKESKGNGEEFINEVASI 140


>ref|XP_006605917.1| PREDICTED: probable receptor-like protein kinase At1g67000-like
           isoform X1 [Glycine max]
          Length = 465

 Score =  101 bits (252), Expect = 9e-20
 Identities = 67/167 (40%), Positives = 83/167 (49%), Gaps = 2/167 (1%)
 Frame = -3

Query: 495 PNCSECQQVRGGSCLEDANGNFQCGSLKNNTVNNTSSDYGAGHGNARRRKAFIIAXXXXX 316
           P+CS C    GG C  D    F C     +T+   +    A  G A     F+I      
Sbjct: 56  PDCSTCIYAHGGQCRLDNTQKFYCYKGNKSTLLIAAI---ASAGGALGAMIFLI------ 106

Query: 315 XXXXXXXXXXXXXLQCWSRKRDLKRTKNA--HLFEDCLQNHPSLTIKRYSFSDIKKMTKS 142
                           W  +R   + KN    + E  L  H  L  KRYS+S+IKK T S
Sbjct: 107 ----------------WCIRRRFYKKKNPTHQIIEMFLNTHGHLAAKRYSYSEIKKATNS 150

Query: 141 FHDKLGQGGYGAVFKGKLLDDGRLVVVKLLTESQGNGQEFINEVASI 1
           F  KLG GGYG+V+KGK L DG LV VK+L++S GNG+EFINEVASI
Sbjct: 151 FRYKLGHGGYGSVYKGK-LQDGSLVAVKVLSDSIGNGEEFINEVASI 196


>ref|XP_006383424.1| hypothetical protein POPTR_0005s15370g [Populus trichocarpa]
           gi|550339034|gb|ERP61221.1| hypothetical protein
           POPTR_0005s15370g [Populus trichocarpa]
          Length = 661

 Score =  101 bits (252), Expect = 9e-20
 Identities = 50/83 (60%), Positives = 67/83 (80%)
 Frame = -3

Query: 249 LKRTKNAHLFEDCLQNHPSLTIKRYSFSDIKKMTKSFHDKLGQGGYGAVFKGKLLDDGRL 70
           LK+ ++    E  + ++ SLT KRYS+SDIKKMTKSF + LG+GG+G V++GKL DDGRL
Sbjct: 300 LKKPQHVGRVETFMMDYHSLTPKRYSYSDIKKMTKSFVNTLGEGGFGNVYRGKLPDDGRL 359

Query: 69  VVVKLLTESQGNGQEFINEVASI 1
           V VK+L ES+G+G+EF+NEVASI
Sbjct: 360 VAVKVLKESKGDGEEFMNEVASI 382


>ref|XP_006442612.1| hypothetical protein CICLE_v10021200mg [Citrus clementina]
           gi|557544874|gb|ESR55852.1| hypothetical protein
           CICLE_v10021200mg [Citrus clementina]
          Length = 320

 Score =  101 bits (251), Expect = 1e-19
 Identities = 53/82 (64%), Positives = 63/82 (76%)
 Frame = -3

Query: 246 KRTKNAHLFEDCLQNHPSLTIKRYSFSDIKKMTKSFHDKLGQGGYGAVFKGKLLDDGRLV 67
           K TK+    E  ++N+ SL  +RYSF ++KKMT SF D+LGQGGYG V+KGK L DGRLV
Sbjct: 30  KATKSDQDLEAFIRNYVSLAPERYSFPNVKKMTNSFKDELGQGGYGRVYKGK-LSDGRLV 88

Query: 66  VVKLLTESQGNGQEFINEVASI 1
            VKLL  S+GNGQEFINEVASI
Sbjct: 89  AVKLLNTSKGNGQEFINEVASI 110


>ref|XP_006430738.1| hypothetical protein CICLE_v10013644mg [Citrus clementina]
           gi|557532795|gb|ESR43978.1| hypothetical protein
           CICLE_v10013644mg [Citrus clementina]
          Length = 640

 Score =  101 bits (251), Expect = 1e-19
 Identities = 65/167 (38%), Positives = 87/167 (52%), Gaps = 4/167 (2%)
 Frame = -3

Query: 489 CSECQQVRGGSCLEDANGNFQC----GSLKNNTVNNTSSDYGAGHGNARRRKAFIIAXXX 322
           C EC+Q  G        G F C    G+  ++  + T     AG   +      I+    
Sbjct: 251 CEECEQSNGNCGYIAKTGEFICYCPNGAYSSSCHSRTDVSLNAGIAASVLGTMVILGILL 310

Query: 321 XXXXXXXXXXXXXXXLQCWSRKRDLKRTKNAHLFEDCLQNHPSLTIKRYSFSDIKKMTKS 142
                           Q   +K+  +  +N   F   ++NH SL  KRYS+SD+K+MTKS
Sbjct: 311 IVI-------------QALIKKKTREDDRNVEAF---IRNHESLAPKRYSYSDVKRMTKS 354

Query: 141 FHDKLGQGGYGAVFKGKLLDDGRLVVVKLLTESQGNGQEFINEVASI 1
           F DKLGQGGYG V+KG+ L DG+LV VK+L  S+ NG+EFINEVASI
Sbjct: 355 FRDKLGQGGYGEVYKGE-LPDGQLVAVKVLKNSRCNGEEFINEVASI 400