BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cocculus23_contig00001687
         (301 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltran...    81   1e-13
gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]        77   2e-12
gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransf...    74   2e-11
dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]       74   3e-11
sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase;...    67   3e-09
gb|ADP76529.1| coclaurine N-methyltransferase [Aristolochia fimb...    59   5e-07
sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberin...    58   2e-06

>sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
           gi|51980204|gb|AAU20766.1| (S)-coclaurine
           N-methyltransferase [Thalictrum flavum subsp. glaucum]
          Length = 361

 Score = 81.3 bits (199), Expect = 1e-13
 Identities = 46/74 (62%), Positives = 56/74 (75%), Gaps = 5/74 (6%)
 Frame = +3

Query: 81  VEG--LKPDKAV---LLKKLELGLIPDEEIKSLIRVQLERRLQWGYKPTYEQRVAQLLGF 245
           VEG  + P KA+   LLKKLELGL+PD+EIK LIR+QL RRLQWG K TYE+++AQL+  
Sbjct: 3   VEGKQVAPKKAIIVELLKKLELGLVPDDEIKKLIRIQLGRRLQWGCKSTYEEQIAQLVNL 62

Query: 246 VRSLCEMSIADTEV 287
             SL +M IA TEV
Sbjct: 63  THSLRQMKIA-TEV 75


>gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]
          Length = 351

 Score = 77.4 bits (189), Expect = 2e-12
 Identities = 36/58 (62%), Positives = 46/58 (79%)
 Frame = +3

Query: 102 KAVLLKKLELGLIPDEEIKSLIRVQLERRLQWGYKPTYEQRVAQLLGFVRSLCEMSIA 275
           K  L KKLELGLIP++E+K L+R+QL RRLQWGYK TYE+++AQ+L    SL  M+IA
Sbjct: 6   KQELFKKLELGLIPNDEVKKLMRIQLARRLQWGYKSTYEEQIAQVLDLTHSLRHMNIA 63


>gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransferase [Papaver
           somniferum] gi|571330880|gb|AHF27396.1|
           S-adenosyl-L-methionine:coclaurine N-methyltransferase
           [synthetic construct]
          Length = 351

 Score = 74.3 bits (181), Expect = 2e-11
 Identities = 35/58 (60%), Positives = 47/58 (81%)
 Frame = +3

Query: 102 KAVLLKKLELGLIPDEEIKSLIRVQLERRLQWGYKPTYEQRVAQLLGFVRSLCEMSIA 275
           K  LL+ +ELGLIPD+EI+ LIRV+LE+RLQWGYK T+E++++QLL  V SL  M +A
Sbjct: 6   KEELLRNMELGLIPDQEIRQLIRVELEKRLQWGYKETHEEQLSQLLDLVHSLKGMKMA 63


>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score = 73.6 bits (179), Expect = 3e-11
 Identities = 40/72 (55%), Positives = 52/72 (72%), Gaps = 3/72 (4%)
 Frame = +3

Query: 81  VEGLKPDKAV---LLKKLELGLIPDEEIKSLIRVQLERRLQWGYKPTYEQRVAQLLGFVR 251
           VE  +  KA    LLK+LELGL+P ++IK LIR +L RRLQWGYKPTYE+++A++     
Sbjct: 3   VEAKQTKKAAIVELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTYEEQIAEIQNLTH 62

Query: 252 SLCEMSIADTEV 287
           SL +M IA TEV
Sbjct: 63  SLRQMKIA-TEV 73


>sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT
           gi|226897760|gb|ACO90251.1| pavine N-methyltransferase
           [Thalictrum flavum]
          Length = 356

 Score = 67.0 bits (162), Expect = 3e-09
 Identities = 28/58 (48%), Positives = 48/58 (82%)
 Frame = +3

Query: 105 AVLLKKLELGLIPDEEIKSLIRVQLERRLQWGYKPTYEQRVAQLLGFVRSLCEMSIAD 278
           A L+K++E G + DEEI+ ++++Q+++RL+WGYKPT+EQ++AQL+ F +SL  M +A+
Sbjct: 12  ANLIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQSLKGMEMAE 69


>gb|ADP76529.1| coclaurine N-methyltransferase [Aristolochia fimbriata]
          Length = 357

 Score = 59.3 bits (142), Expect = 5e-07
 Identities = 28/62 (45%), Positives = 45/62 (72%), Gaps = 2/62 (3%)
 Frame = +3

Query: 93  KPDKAVLLKKLELGLIPDEEIKSLIRVQLERRLQW--GYKPTYEQRVAQLLGFVRSLCEM 266
           K +K  +L++LE G +PDEE + LIR++L RRL+W    KPTYE++ A+++  V++L +M
Sbjct: 5   KLNKTEMLRRLEEGSVPDEEFRRLIRIELGRRLRWYCQKKPTYEEQTAEIVALVKALRQM 64

Query: 267 SI 272
            I
Sbjct: 65  GI 66


>sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
           Short=EcTNMT gi|226897702|gb|ACO90222.1|
           tetrahydroprotoberberine N-methyltransferase
           [Eschscholzia californica]
          Length = 350

 Score = 57.8 bits (138), Expect = 2e-06
 Identities = 29/66 (43%), Positives = 46/66 (69%), Gaps = 3/66 (4%)
 Frame = +3

Query: 111 LLKKLELGLIPDEEIKSLIRVQLERRLQWGYKPTYEQRVAQLLGFVRSLCEMSIA---DT 281
           ++ +L  G I DEE+K LIR Q +RR++WGYKPT+E+++A  L F++ L EM ++   DT
Sbjct: 8   IMGRLMKGEIEDEELKKLIRHQWDRRIEWGYKPTHEKQLAFNLDFIKGLKEMVMSGEIDT 67

Query: 282 EVNDTF 299
              +T+
Sbjct: 68  MNKETY 73