BLASTX nr result
ID: Cinnamomum25_contig00011079
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cinnamomum25_contig00011079
(529 letters)
Database: ./nr
69,698,275 sequences; 24,982,196,650 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase ... 105 2e-20
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 105 2e-20
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 102 8e-20
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi... 100 6e-19
gb|AFK73713.1| cytochrome P450 [Papaver somniferum] 99 1e-18
dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss... 99 1e-18
gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 97 4e-18
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla... 97 4e-18
ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 96 9e-18
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 96 9e-18
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P... 96 1e-17
ref|XP_006353352.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 95 2e-17
ref|XP_004234364.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 95 2e-17
ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 94 3e-17
ref|XP_002513351.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 94 4e-17
gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum] 94 5e-17
gb|KDP34501.1| hypothetical protein JCGZ_11051 [Jatropha curcas] 93 8e-17
ref|XP_009757123.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 92 1e-16
ref|XP_009610421.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 92 1e-16
ref|XP_011081970.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 92 2e-16
>gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
californica [Expression vector pWCD2353]
Length = 490
Score = 105 bits (261), Expect = 2e-20
Identities = 67/192 (34%), Positives = 98/192 (51%), Gaps = 16/192 (8%)
Frame = -1
Query: 529 TYRIKLYIYHSLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSS 350
++R+K ++ +S+ +S DC+E WK LR C+TELFT +M++ QAH+R+ K E+V L
Sbjct: 101 SFRVKGHVENSIVWS-DCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKK 159
Query: 349 RGEVVNLGELVFGTSFNILGNLLFSRDVFVLGDGENSG---LKHAFSR-----------S 212
+GE V + E++FGT NI GNL+FS+++F LGD NSG K R
Sbjct: 160 QGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGD-PNSGSSEFKEYLWRMLELGNSTNPAD 218
Query: 211 XXXXXXXXXXXXXXKEATGIVKKIYGIW--XXXXXXXXXXXXXXXXXRDFVDVLQSAGCS 38
KE +K IY IW DFVDV +G +
Sbjct: 219 YFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLN 278
Query: 37 EIQIKALCLDMF 2
+ QI AL +++F
Sbjct: 279 DYQINALLMELF 290
>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1, partial
[Eschscholzia californica] gi|3127027|gb|AAC39452.1|
(S)-N-methylcoclaurine 3'-hydroxylase [Eschscholzia
californica]
Length = 487
Score = 105 bits (261), Expect = 2e-20
Identities = 67/192 (34%), Positives = 98/192 (51%), Gaps = 16/192 (8%)
Frame = -1
Query: 529 TYRIKLYIYHSLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSS 350
++R+K ++ +S+ +S DC+E WK LR C+TELFT +M++ QAH+R+ K E+V L
Sbjct: 100 SFRVKGHVENSIVWS-DCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKK 158
Query: 349 RGEVVNLGELVFGTSFNILGNLLFSRDVFVLGDGENSG---LKHAFSR-----------S 212
+GE V + E++FGT NI GNL+FS+++F LGD NSG K R
Sbjct: 159 QGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGD-PNSGSSEFKEYLWRMLELGNSTNPAD 217
Query: 211 XXXXXXXXXXXXXXKEATGIVKKIYGIW--XXXXXXXXXXXXXXXXXRDFVDVLQSAGCS 38
KE +K IY IW DFVDV +G +
Sbjct: 218 YFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLN 277
Query: 37 EIQIKALCLDMF 2
+ QI AL +++F
Sbjct: 278 DYQINALLMELF 289
>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
AltName: Full=Cytochrome P450 80B2 [Eschscholzia
californica] gi|3127029|gb|AAC39453.1|
(S)-N-methylcoclaurine 3'-hydroxylase [Eschscholzia
californica]
Length = 488
Score = 102 bits (255), Expect = 8e-20
Identities = 64/191 (33%), Positives = 97/191 (50%), Gaps = 15/191 (7%)
Frame = -1
Query: 529 TYRIKLYIYHSLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSS 350
++R+K ++ +S+ +S DC+E WK LR C+TELFT +M++ QAH+R+ K E+V L
Sbjct: 101 SFRVKGHVENSIVWS-DCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKK 159
Query: 349 RGEVVNLGELVFGTSFNILGNLLFSRDVFVLG--DGENSGLKHAFSR-----------SX 209
+GE V + E++FGT NI GNL+FS+++F LG + +S K R
Sbjct: 160 QGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADY 219
Query: 208 XXXXXXXXXXXXXKEATGIVKKIYGIW--XXXXXXXXXXXXXXXXXRDFVDVLQSAGCSE 35
KE +K IY IW DFVDV +G ++
Sbjct: 220 FPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLND 279
Query: 34 IQIKALCLDMF 2
QI AL +++F
Sbjct: 280 YQINALLMELF 290
>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 99.8 bits (247), Expect = 6e-19
Identities = 62/189 (32%), Positives = 99/189 (52%), Gaps = 13/189 (6%)
Frame = -1
Query: 529 TYRIKLYIYHSLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSS 350
++R+K ++ +S+ +S +C++ WK LR C+TELFT +M++ Q+ +R+ K E+V L S
Sbjct: 101 SFRVKEHVENSIVWS-ECNDNWKLLRKVCRTELFTPKMIESQSEIREGKAREMVKFLRSK 159
Query: 349 RGEVVNLGELVFGTSFNILGNLLFSRDVFVLGD--GENSGLKHAFSR-----------SX 209
GEVV + E+VFGT NI GNL+FS+DVF L D G + LK +
Sbjct: 160 EGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNPADY 219
Query: 208 XXXXXXXXXXXXXKEATGIVKKIYGIWXXXXXXXXXXXXXXXXXRDFVDVLQSAGCSEIQ 29
+ ++++IY +W DFVDVL +AG + +
Sbjct: 220 FPIMGKYDLFGQRRAVADVLQQIYDVW-GINLKERRGMKGSESNNDFVDVLLNAGLEDQK 278
Query: 28 IKALCLDMF 2
I AL +++F
Sbjct: 279 INALLMELF 287
>gb|AFK73713.1| cytochrome P450 [Papaver somniferum]
Length = 440
Score = 99.0 bits (245), Expect = 1e-18
Identities = 59/191 (30%), Positives = 97/191 (50%), Gaps = 15/191 (7%)
Frame = -1
Query: 529 TYRIKLYIYHSLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSS 350
++R+K ++ +S+ ++ +C+E WK LR C+T+LFT +M++ QA +R+ K +E+V L +
Sbjct: 101 SFRVKNHVENSIVWN-ECNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKN 159
Query: 349 RGEVVNLGELVFGTSFNILGNLLFSRDVFVLGDGENSGLK-------------HAFSRSX 209
G V + E++FGT N+ GNL+FS+D+F LGD + L+ A
Sbjct: 160 EGIEVKIVEVIFGTLVNMFGNLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDY 219
Query: 208 XXXXXXXXXXXXXKEATGIVKKIYGIW--XXXXXXXXXXXXXXXXXRDFVDVLQSAGCSE 35
K+ ++ +YG+W DFVDVL +G +
Sbjct: 220 VPLLGSLDLFGQRKDVADCLQGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDD 279
Query: 34 IQIKALCLDMF 2
QI +L LDMF
Sbjct: 280 QQINSLLLDMF 290
>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
Length = 486
Score = 99.0 bits (245), Expect = 1e-18
Identities = 47/98 (47%), Positives = 70/98 (71%)
Frame = -1
Query: 529 TYRIKLYIYHSLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSS 350
++R+K +I +SL +S DC++ WK LR +TE+F+ +ML Q+H+R+ KV EL+ L S
Sbjct: 104 SFRLKHHIKYSLVWS-DCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSK 162
Query: 349 RGEVVNLGELVFGTSFNILGNLLFSRDVFVLGDGENSG 236
G+VV + + VFGT NILGN++FS+DVFV GD + G
Sbjct: 163 EGQVVKISQFVFGTLLNILGNVVFSKDVFVYGDETDKG 200
>gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 97.1 bits (240), Expect = 4e-18
Identities = 61/191 (31%), Positives = 100/191 (52%), Gaps = 15/191 (7%)
Frame = -1
Query: 529 TYRIKLYIYHSLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSS 350
++R+K ++ +S+ +S +C+E WK LR C+TELFT +M++ QA +R++K +E+V L +
Sbjct: 95 SFRVKEHVENSIVWS-ECNETWKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKN 153
Query: 349 RGEVVNLGELVFGTSFNILGNLLFSRDVFVLGDGENSG---LKHAFSR-----------S 212
G V + E+VFGT NI GNL+FS+++F LGD E+SG +K R
Sbjct: 154 VGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGD-ESSGSVEMKEHLWRMLELGNSTNPAD 212
Query: 211 XXXXXXXXXXXXXXKEATGIVKKIYGIW-XXXXXXXXXXXXXXXXXRDFVDVLQSAGCSE 35
K+ ++ IY +W DFV++L +G +
Sbjct: 213 YFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDD 272
Query: 34 IQIKALCLDMF 2
QI AL +++F
Sbjct: 273 QQINALLMEIF 283
>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase
isozyme 2; AltName: Full=Cytochrome P450 80B2 [Coptis
japonica] gi|9971208|dbj|BAB12433.1|
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 97.1 bits (240), Expect = 4e-18
Identities = 61/189 (32%), Positives = 98/189 (51%), Gaps = 13/189 (6%)
Frame = -1
Query: 529 TYRIKLYIYHSLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSS 350
++R+K ++ +S+ +S +C++ WK LR C+TELFT +M++ Q+ +R+ K E+V L
Sbjct: 101 SFRVKEHVENSIVWS-ECNDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGK 159
Query: 349 RGEVVNLGELVFGTSFNILGNLLFSRDVFVLGD--GENSGLKHAFSR-----------SX 209
GEVV + E+VFGT NI GNL+FS+DVF L D G + LK +
Sbjct: 160 EGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADY 219
Query: 208 XXXXXXXXXXXXXKEATGIVKKIYGIWXXXXXXXXXXXXXXXXXRDFVDVLQSAGCSEIQ 29
+ ++++IY +W DFVDVL +AG + +
Sbjct: 220 FPIMGKLDLFGQRRAVAEVLQQIYDVW-GVMLKERRGTKGSESKNDFVDVLLNAGLDDQK 278
Query: 28 IKALCLDMF 2
I AL +++F
Sbjct: 279 INALLMELF 287
>ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
communis] gi|223547255|gb|EEF48750.1|
(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
[Ricinus communis]
Length = 496
Score = 95.9 bits (237), Expect = 9e-18
Identities = 55/178 (30%), Positives = 92/178 (51%), Gaps = 12/178 (6%)
Frame = -1
Query: 499 SLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGEL 320
+L ++ CS++WK LRA C++ELF+ + ++ QA LR+ K+ E++ L S +G++VN+GE+
Sbjct: 121 ALVWNPSCSDQWKFLRAMCRSELFSAKAIESQATLREKKLTEMLDFLTSKQGQIVNIGEV 180
Query: 319 VFGTSFNILGNLLFSRDVFVLGDGENSG-LKHAFS-----------RSXXXXXXXXXXXX 176
VF T+FN + NLLFS+D+ D N+G LK S
Sbjct: 181 VFTTAFNTISNLLFSKDLLSFEDQGNAGELKTLISTLMELATCPNIADFYPVLTKLDPQG 240
Query: 175 XXKEATGIVKKIYGIWXXXXXXXXXXXXXXXXXRDFVDVLQSAGCSEIQIKALCLDMF 2
++ +++++G+W DF+DV S G + QI L L++F
Sbjct: 241 IKRKMKNCLERMFGVWDIYIKERRERHVKDARKTDFLDVFLSNGFDDHQINWLLLELF 298
>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 95.9 bits (237), Expect = 9e-18
Identities = 60/191 (31%), Positives = 100/191 (52%), Gaps = 15/191 (7%)
Frame = -1
Query: 529 TYRIKLYIYHSLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSS 350
++R+K ++ +S+ +S +C+E WK LR C+T+LFT +M++ QA +R++K +E+V L +
Sbjct: 95 SFRVKEHVENSIVWS-ECNETWKKLRKVCRTDLFTQKMIESQAEVRESKAMEMVEYLKKN 153
Query: 349 RGEVVNLGELVFGTSFNILGNLLFSRDVFVLGDGENSG---LKHAFSR-----------S 212
G V + E+VFGT NI GNL+FS+++F LGD E+SG +K R
Sbjct: 154 VGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGD-ESSGSVEMKEHLWRMLELGNSTNPAD 212
Query: 211 XXXXXXXXXXXXXXKEATGIVKKIYGIW-XXXXXXXXXXXXXXXXXRDFVDVLQSAGCSE 35
K+ ++ IY +W DFV++L +G +
Sbjct: 213 YFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKESKIAKQHNNSKKNDFVEILLDSGLDD 272
Query: 34 IQIKALCLDMF 2
QI AL +++F
Sbjct: 273 QQINALLMEIF 283
>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
Length = 486
Score = 95.5 bits (236), Expect = 1e-17
Identities = 60/191 (31%), Positives = 100/191 (52%), Gaps = 15/191 (7%)
Frame = -1
Query: 529 TYRIKLYIYHSLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSS 350
++R+K ++ +S+ +S +C+E WK LR C+ ELFT +M++ QA +R++K +E+V L +
Sbjct: 100 SFRVKEHVENSIVWS-ECNETWKKLRKVCRAELFTQKMIESQAEIRESKAMEMVEFLKRN 158
Query: 349 RGEVVNLGELVFGTSFNILGNLLFSRDVFVLGDGENSG---LKHAFSR-----------S 212
+G V + E+VFGT NI GNL+FS+++F LGD E+SG +K R
Sbjct: 159 QGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGD-ESSGSVEMKEHLWRMLELGNSTNPAD 217
Query: 211 XXXXXXXXXXXXXXKEATGIVKKIYGIW-XXXXXXXXXXXXXXXXXRDFVDVLQSAGCSE 35
K+ ++ IY +W DFV++L +G +
Sbjct: 218 YFPFLGRFDLFGQRKDVADCLQGIYSVWGAMLKERKIAKLHNNSKKNDFVEILLDSGLDD 277
Query: 34 IQIKALCLDMF 2
QI AL +++F
Sbjct: 278 QQINALLMEIF 288
>ref|XP_006353352.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 486
Score = 95.1 bits (235), Expect = 2e-17
Identities = 59/187 (31%), Positives = 94/187 (50%), Gaps = 12/187 (6%)
Frame = -1
Query: 529 TYRIKLYIYHSLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSS 350
TY + + HS+ FS D S WK LRAFC+T LF+ + ++ QA LR+ KV E++ L S
Sbjct: 105 TYELSVIDQHSIVFSSDLSNHWKFLRAFCRTHLFSPKAVESQAALREKKVSEMIHFLRSR 164
Query: 349 RGEVVNLGELVFGTSFNILGNLLFSRDVFVLG-DGENSGLKHAFSR----------SXXX 203
+GE V + E++F T N LGNL FS+D+ L + SG+KH + S
Sbjct: 165 KGETVKISEIMFATILNTLGNLFFSKDLCDLDYETNTSGIKHVIRKFVELGAMPNISEFY 224
Query: 202 XXXXXXXXXXXKEATGIVK-KIYGIWXXXXXXXXXXXXXXXXXRDFVDVLQSAGCSEIQI 26
++ T I + +++ IW DF+DV+ +G S++Q+
Sbjct: 225 PLFDALDLQGLRKQTEIYQNQLFNIW--SDIVKEKRQAISRSSSDFLDVMIDSGFSDLQV 282
Query: 25 KALCLDM 5
L +++
Sbjct: 283 DILLMEL 289
>ref|XP_004234364.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Solanum lycopersicum]
Length = 486
Score = 94.7 bits (234), Expect = 2e-17
Identities = 47/106 (44%), Positives = 68/106 (64%), Gaps = 1/106 (0%)
Frame = -1
Query: 529 TYRIKLYIYHSLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSS 350
TY + + HS+ FS D S +WK LRAFC+T LF+ + ++ QA LR+ KV E++ L S
Sbjct: 105 TYELSVIDQHSIVFSSDLSNQWKFLRAFCRTHLFSPKAVESQAALREKKVSEMMNFLRSR 164
Query: 349 RGEVVNLGELVFGTSFNILGNLLFSRDVFVLG-DGENSGLKHAFSR 215
+GE V + E++FGT N LGNL FS+D+ L + SG+KH +
Sbjct: 165 KGETVKISEILFGTILNTLGNLFFSKDLCDLDYETNTSGIKHVIRK 210
>ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 485
Score = 94.4 bits (233), Expect = 3e-17
Identities = 63/187 (33%), Positives = 92/187 (49%), Gaps = 13/187 (6%)
Frame = -1
Query: 523 RIKLYIYHSLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSSRG 344
RIK YI HS+ ++ DC++ WK +R +TELF+T+MLD+QAH R+ KV EL+ L G
Sbjct: 103 RIKGYIEHSMVWA-DCTDYWKMVRKVWRTELFSTKMLDIQAHAREEKVSELMKFLIRKEG 161
Query: 343 EVVNLGELVFGTSFNILGNLLFSRDVFVLGD--GENSGLKHAFSR-----------SXXX 203
E VN +++FG+ NILG L+FS+DV+ D N G+K +
Sbjct: 162 EKVNFADVIFGSILNILGALIFSKDVYDFEDRTDNNLGMKGMIRQLMILAAIPNLADLYP 221
Query: 202 XXXXXXXXXXXKEATGIVKKIYGIWXXXXXXXXXXXXXXXXXRDFVDVLQSAGCSEIQIK 23
K + VK++ W DF+ VL +G S+ QI
Sbjct: 222 ILGGSDFQGLRKASAACVKRMNESW--AAIVKQRRKNDDHSKNDFLQVLLDSGFSDPQID 279
Query: 22 ALCLDMF 2
A+ L+ F
Sbjct: 280 AMLLETF 286
>ref|XP_002513351.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
communis] gi|223547259|gb|EEF48754.1|
(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
[Ricinus communis]
Length = 318
Score = 94.0 bits (232), Expect = 4e-17
Identities = 46/102 (45%), Positives = 71/102 (69%), Gaps = 1/102 (0%)
Frame = -1
Query: 526 YRIKLYIYHSLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSSR 347
Y+I ++ ++ C+ERWK+LRA C+TELF+ ++ QA LR+ K++E+V L S +
Sbjct: 119 YKIHFVDRLAIVWASSCNERWKSLRALCRTELFSANAIESQAALREKKMIEMVEYLSSEQ 178
Query: 346 GEVVNLGELVFGTSFNILGNLLFSRDVFVLGDGE-NSGLKHA 224
G+VVN+GE+VF + FN + NL+FS+D+ D E SGLK+A
Sbjct: 179 GQVVNIGEVVFTSVFNTISNLIFSKDLLSFEDQERGSGLKNA 220
>gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum]
Length = 483
Score = 93.6 bits (231), Expect = 5e-17
Identities = 48/97 (49%), Positives = 69/97 (71%), Gaps = 2/97 (2%)
Frame = -1
Query: 529 TYRIKLYIYHSLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSS 350
++R+K +I +SL ++ DCSE WK LR +TE+F+ +ML +Q H+R+ KV ELV L
Sbjct: 102 SFRLKHHIKYSLVWA-DCSEYWKLLRKIVRTEIFSPKMLQVQEHVREQKVAELVEFLKGK 160
Query: 349 RGEVVNLGELVFGTSFNILGNLLFSRDVF--VLGDGE 245
G+VV + +LVFGT NILGN++FS+DVF V DG+
Sbjct: 161 EGQVVKISQLVFGTLLNILGNVIFSKDVFRYVGADGD 197
>gb|KDP34501.1| hypothetical protein JCGZ_11051 [Jatropha curcas]
Length = 444
Score = 92.8 bits (229), Expect = 8e-17
Identities = 58/179 (32%), Positives = 90/179 (50%), Gaps = 13/179 (7%)
Frame = -1
Query: 499 SLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGEL 320
SL ++ C+E+WK+ RA C+TELF+ + ++ +A LR+NK+ E+V L S GE VN+GE+
Sbjct: 118 SLVWNPTCNEQWKSFRALCRTELFSAKAVESRASLRENKMTEMVEYLSSKEGEEVNIGEV 177
Query: 319 VFGTSFNILGNLLFSRDVFVLGDGENSGLKHAFSR-----------SXXXXXXXXXXXXX 173
VF T FN + NLLFS+D+ L +N GLK +
Sbjct: 178 VFATVFNTISNLLFSKDLISL---QNQGLKSFLWKMMELGAAPNVADFYPTFAELDPQGI 234
Query: 172 XKEATGIVKKIYGIW--XXXXXXXXXXXXXXXXXRDFVDVLQSAGCSEIQIKALCLDMF 2
+ + +K+++GIW DF+DV S G + QI L +++F
Sbjct: 235 RRAISECLKEMFGIWEIYIKERRERHANAHNVFSTDFLDVFLSNGLDDDQINWLVMELF 293
>ref|XP_009757123.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
isoform X1 [Nicotiana sylvestris]
gi|698520618|ref|XP_009757124.1| PREDICTED: probable
(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 isoform
X2 [Nicotiana sylvestris]
Length = 489
Score = 92.4 bits (228), Expect = 1e-16
Identities = 44/105 (41%), Positives = 68/105 (64%), Gaps = 1/105 (0%)
Frame = -1
Query: 526 YRIKLYIYHSLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSSR 347
Y++ + HSL +S D S WK+LRAFC+T LF+ + ++ QA LR+ KV E++ L S +
Sbjct: 105 YKLSVVDQHSLVWSSDLSNHWKSLRAFCRTHLFSAKAVESQAALREKKVFEMIEFLRSKK 164
Query: 346 GEVVNLGELVFGTSFNILGNLLFSRDVFVLG-DGENSGLKHAFSR 215
G+ V + E++F T N LGNL F++D+ L +G SG+KH +
Sbjct: 165 GKTVKISEIMFATILNTLGNLFFTKDLCDLDYEGNTSGIKHVIRK 209
>ref|XP_009610421.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nicotiana tomentosiformis]
Length = 489
Score = 92.0 bits (227), Expect = 1e-16
Identities = 54/188 (28%), Positives = 97/188 (51%), Gaps = 14/188 (7%)
Frame = -1
Query: 526 YRIKLYIYHSLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSSR 347
Y++ + HS+ +S D + WK+LRAFC+T LF+ + ++ QA LR+ KV E++ L S++
Sbjct: 105 YKLSVIDQHSIVWSSDLTNHWKSLRAFCRTHLFSAKAVESQADLREKKVFEMIEFLRSNK 164
Query: 346 GEVVNLGELVFGTSFNILGNLLFSRDVFVL-GDGENSGLKHAFSR----------SXXXX 200
G+ V + E++F T N LGNL F++++ L +G SG+KH R S
Sbjct: 165 GKTVKISEILFATILNTLGNLFFTKNLCDLDSEGNTSGIKHVIRRFVELGAMPNISEFYP 224
Query: 199 XXXXXXXXXXKEATGIVK-KIYGIW--XXXXXXXXXXXXXXXXXRDFVDVLQSAGCSEIQ 29
++ T I + ++ IW +DF+D++ +G S++Q
Sbjct: 225 FLDALDLQGLRKLTNIYQDQLISIWAEVVKEKRQAISCGSSNKNQDFLDIMIDSGFSDLQ 284
Query: 28 IKALCLDM 5
I + +++
Sbjct: 285 INIILMEL 292
>ref|XP_011081970.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Sesamum indicum]
Length = 509
Score = 91.7 bits (226), Expect = 2e-16
Identities = 59/179 (32%), Positives = 86/179 (48%), Gaps = 14/179 (7%)
Frame = -1
Query: 499 SLPFSFDCSERWKTLRAFCKTELFTTRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGEL 320
SL ++ DCS +WK++R K ELF+ + L+ QA LR+ KV E+V L GEVV +GE+
Sbjct: 131 SLLWATDCSPKWKSIRVLWKGELFSNKALEAQACLREKKVAEMVEYLGRKEGEVVKIGEV 190
Query: 319 VFGTSFNILGNLLFSRDVFVLGDGENS-GLKHAFSR-------------SXXXXXXXXXX 182
VF +N LGNL FSRD+ LGD + + K F R
Sbjct: 191 VFAAVYNTLGNLCFSRDLIGLGDEKMARDWKEVFWRFMECATTPLIADFFPVLDGLGLDF 250
Query: 181 XXXXKEATGIVKKIYGIWXXXXXXXXXXXXXXXXXRDFVDVLQSAGCSEIQIKALCLDM 5
K+A +KK++G+W DF+D + G +IQI + L++
Sbjct: 251 QGKKKQAAKCMKKMFGVWQELIRQRRESADDSKKHGDFLDFMLENGFLDIQILFMLLEI 309