BLASTX nr result
ID: Cinnamomum25_contig00010999
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cinnamomum25_contig00010999
(710 letters)
Database: ./nr
69,698,275 sequences; 24,982,196,650 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_010934155.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 223 6e-56
ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 221 2e-55
ref|XP_010662515.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 217 5e-54
emb|CBI31830.3| unnamed protein product [Vitis vinifera] 217 5e-54
ref|XP_010112571.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 214 4e-53
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum... 214 5e-53
ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 213 1e-52
ref|XP_002298058.1| hypothetical protein POPTR_0001s08860g [Popu... 213 1e-52
emb|CAN62646.1| hypothetical protein VITISV_013218 [Vitis vinifera] 213 1e-52
ref|XP_002509713.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 211 3e-52
ref|XP_010112567.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 211 3e-52
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi... 211 3e-52
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla... 211 4e-52
ref|XP_012087221.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 210 6e-52
ref|XP_011029198.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 209 1e-51
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 209 2e-51
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 208 3e-51
ref|XP_011041604.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 207 4e-51
gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase ... 207 5e-51
gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 207 5e-51
>ref|XP_010934155.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Elaeis guineensis]
Length = 338
Score = 223 bits (569), Expect = 6e-56
Identities = 106/181 (58%), Positives = 135/181 (74%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
+N +++ +R ELE+ IGE VRESHL L YL ACIKE+ EA
Sbjct: 161 KNKPILENLRSELEREIGED-----IVRESHLPHLHYLHACIKETQRLHPAVPLLVH-EA 214
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
L TCE MD T+PKGC V+VNVWAIGRDP+ WEDPLTFSP+RF++SS+ YKGNDF+F PFG
Sbjct: 215 LQTCETMDCTIPKGCHVLVNVWAIGRDPETWEDPLTFSPQRFIDSSMGYKGNDFKFIPFG 274
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
GRR CPG+PL +++V LI+ASLIH+F+WS+P GM L+MDE+FGLTL ++HPLLL+P
Sbjct: 275 EGRRSCPGVPLVNQIVPLILASLIHSFEWSMPHGMDSGHLDMDEKFGLTLHKRHPLLLVP 334
Query: 168 K 166
+
Sbjct: 335 R 335
>ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 485
Score = 221 bits (564), Expect = 2e-55
Identities = 105/181 (58%), Positives = 138/181 (76%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
RNP+ + KVR+EL++VI RR V+ES L L YL AC+KE+ A
Sbjct: 306 RNPEKLVKVREELDRVI--RRSNN--VKESDLPNLPYLHACVKETLRLHPPVTFLLPHRA 361
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
++TC++M+YT+PKGC++MVN +AIGRD K WE PL+F PERFLNS +DY+GNDF++ PFG
Sbjct: 362 METCQMMNYTIPKGCQLMVNTYAIGRDSKTWEKPLSFLPERFLNSELDYQGNDFQYIPFG 421
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
AGRRICPGL L +RVV LI+ASL+HTFDWSLPDGM P+EL+M+++FGL L + PL++IP
Sbjct: 422 AGRRICPGLSLATRVVRLILASLLHTFDWSLPDGMHPDELDMNDKFGLALQKDIPLVVIP 481
Query: 168 K 166
K
Sbjct: 482 K 482
>ref|XP_010662515.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 493
Score = 217 bits (553), Expect = 5e-54
Identities = 99/181 (54%), Positives = 135/181 (74%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
RNP VM K+RDEL ++IGE VRESHL L YL+AC+KE+ A
Sbjct: 315 RNPDVMYKLRDELTKIIGES-----PVRESHLPHLPYLQACVKETLRLHPPAPLLLPHRA 369
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
++TC+VM YT+PK +V VN+WA+GRDPKVW+DPL+F+PERFL+S +++KGNDFE+ PFG
Sbjct: 370 METCQVMGYTIPKDSQVFVNIWAMGRDPKVWDDPLSFTPERFLDSKLEFKGNDFEYIPFG 429
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
AGRRICPG+ LG+R V L++A+L+H FDWSLPD M +++M+E +TL +++PL L+P
Sbjct: 430 AGRRICPGMALGARQVPLVLATLVHLFDWSLPDNMDSAQIDMEEWLVITLRKENPLRLVP 489
Query: 168 K 166
K
Sbjct: 490 K 490
>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
Length = 1538
Score = 217 bits (553), Expect = 5e-54
Identities = 99/181 (54%), Positives = 135/181 (74%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
RNP VM K+RDEL ++IGE VRESHL L YL+AC+KE+ A
Sbjct: 1360 RNPDVMYKLRDELTKIIGES-----PVRESHLPHLPYLQACVKETLRLHPPAPLLLPHRA 1414
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
++TC+VM YT+PK +V VN+WA+GRDPKVW+DPL+F+PERFL+S +++KGNDFE+ PFG
Sbjct: 1415 METCQVMGYTIPKDSQVFVNIWAMGRDPKVWDDPLSFTPERFLDSKLEFKGNDFEYIPFG 1474
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
AGRRICPG+ LG+R V L++A+L+H FDWSLPD M +++M+E +TL +++PL L+P
Sbjct: 1475 AGRRICPGMALGARQVPLVLATLVHLFDWSLPDNMDSAQIDMEEWLVITLRKENPLRLVP 1534
Query: 168 K 166
K
Sbjct: 1535 K 1535
Score = 202 bits (515), Expect = 1e-49
Identities = 94/181 (51%), Positives = 125/181 (69%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
RN M K+R EL Q++GE VRESHL RL YL+AC+KE+ A
Sbjct: 860 RNQDAMDKLRGELRQIVGES-----PVRESHLPRLPYLQACVKEALRLHPPAPLLLPHLA 914
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
+TC+VM YT+PK ++ VN+WA+ RDPK+W+DPL+F PERFL+S +D+KGNDFE+ PFG
Sbjct: 915 AETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLSFKPERFLDSKLDFKGNDFEYIPFG 974
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
AGRRICPGL LG R V LI+A+ +H F WSLP M +L+M+E +TL ++ PL L+P
Sbjct: 975 AGRRICPGLALGGRQVPLILATFVHLFGWSLPGNMDSAQLDMEEWLVITLRKEQPLRLVP 1034
Query: 168 K 166
+
Sbjct: 1035 R 1035
Score = 193 bits (490), Expect = 9e-47
Identities = 89/181 (49%), Positives = 124/181 (68%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
+N M K+ +EL Q+IGE VRESHL L YL+AC+KE+ A
Sbjct: 319 KNQDAMHKLCNELTQIIGES-----PVRESHLPHLPYLQACVKETLRLHPTGPLLLPHRA 373
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
+TC++M YT+PK + VN+WA+GRDP WEDPL+F PERFL+S +++KGNDFE+ PFG
Sbjct: 374 TETCQIMGYTIPKDSIIFVNMWAMGRDPGTWEDPLSFKPERFLDSKLEFKGNDFEYIPFG 433
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
AGRR+CPG+PL +R+V +I+A+ + FDWS P M E++M+ERF +TL ++ PL L+
Sbjct: 434 AGRRMCPGMPLAARLVPMILATFVRLFDWSTPGDMDFAEIDMEERFVITLRKEQPLRLLK 493
Query: 168 K 166
K
Sbjct: 494 K 494
>ref|XP_010112571.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Morus notabilis]
gi|587947841|gb|EXC34118.1| (S)-N-methylcoclaurine
3'-hydroxylase isozyme 2 [Morus notabilis]
Length = 499
Score = 214 bits (545), Expect = 4e-53
Identities = 98/181 (54%), Positives = 135/181 (74%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
+NPK M+ V+ EL + I ++ VRESHL +L YL+AC+KE+ A
Sbjct: 320 KNPKCMKIVKAELAREIVDQD----MVRESHLPKLPYLQACVKETLRLHPPAPFLLPHRA 375
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
+++C+V++YT+PK +++VNVWAIGRDP W+DPLTF PERFL SS+D+KGNDFEF PFG
Sbjct: 376 VESCQVLNYTIPKDSQLLVNVWAIGRDPMYWKDPLTFKPERFLESSLDFKGNDFEFLPFG 435
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
AGRRICPGLP+ ++ V L++ASLIH DWSLP G P ++NM E++G+TL+++ PL+LIP
Sbjct: 436 AGRRICPGLPMAAKHVPLVVASLIHFLDWSLPYGKDPLDMNMSEKYGITLIKEEPLILIP 495
Query: 168 K 166
K
Sbjct: 496 K 496
>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 214 bits (544), Expect = 5e-53
Identities = 100/180 (55%), Positives = 130/180 (72%)
Frame = -3
Query: 705 NPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREAL 526
N +V+ K+R EL V+G + V+ES++ L YL+A +KE+ R AL
Sbjct: 308 NLRVISKLRAELINVVGHK-----TVKESNIPHLPYLQAIVKETLRLHPPTPLLLPRRAL 362
Query: 525 DTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFGA 346
+TC+VM+YT+PK C++MVN WAIGRDPK W+DPLTF PERF+NS+VDYKGNDFE PFG
Sbjct: 363 ETCKVMNYTIPKECQIMVNAWAIGRDPKTWDDPLTFKPERFMNSTVDYKGNDFELIPFGG 422
Query: 345 GRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIPK 166
GRRICPGLPL S+ + LI+A+L+ FDWS P GM PNE+ MDE+FGL L + PLL++ K
Sbjct: 423 GRRICPGLPLASQFLSLIVATLVQNFDWSFPQGMTPNEVPMDEKFGLPLQKDPPLLIVLK 482
>ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
communis] gi|223549945|gb|EEF51432.1|
(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
[Ricinus communis]
Length = 496
Score = 213 bits (541), Expect = 1e-52
Identities = 98/181 (54%), Positives = 134/181 (74%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
+N + M+KVR+EL++ I + ++ESH+++L YL AC+KE+ R A
Sbjct: 317 KNKEAMKKVREELDREINKN-----PIKESHVSQLPYLNACVKETLRLHPPAPFLIPRRA 371
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
+ CEVM+YT+PK +V+VNVWAIGRDP VWEDP +F PERFL SS+D KG+DFE PFG
Sbjct: 372 TENCEVMNYTIPKDSQVLVNVWAIGRDPSVWEDPSSFKPERFLGSSLDVKGHDFELIPFG 431
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
+GRRICPGLP+ +R + L++ASLIH FDWSLP+G P +++M E+FG+TL +HPLL+IP
Sbjct: 432 SGRRICPGLPMATRQLSLVLASLIHCFDWSLPNGGDPAKVDMTEKFGITLQMEHPLLIIP 491
Query: 168 K 166
K
Sbjct: 492 K 492
>ref|XP_002298058.1| hypothetical protein POPTR_0001s08860g [Populus trichocarpa]
gi|222845316|gb|EEE82863.1| hypothetical protein
POPTR_0001s08860g [Populus trichocarpa]
Length = 507
Score = 213 bits (541), Expect = 1e-52
Identities = 97/180 (53%), Positives = 135/180 (75%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
+NP+ ++KV++E+ VI R+ + +ESHL +LTYL+AC+KE+ A
Sbjct: 326 KNPRCLKKVQEEIANVINMNRDTGF--KESHLPQLTYLQACVKETLRLHPPGPFLLPHRA 383
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
+D+C+VM+YT+PK +V+VN WAIGRDPK WE+P+ F+PERFL+S++D+KGNDFEF PFG
Sbjct: 384 IDSCQVMNYTIPKNTQVLVNYWAIGRDPKSWEEPVVFNPERFLSSNLDFKGNDFEFIPFG 443
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
+GRRICPGLP+ ++ V LIIA LI FDWSLP G P +L+M E +GLTL ++ PLLL+P
Sbjct: 444 SGRRICPGLPMAAKHVALIIAYLILFFDWSLPCGKNPTDLDMSENYGLTLRKEQPLLLVP 503
>emb|CAN62646.1| hypothetical protein VITISV_013218 [Vitis vinifera]
Length = 475
Score = 213 bits (541), Expect = 1e-52
Identities = 97/181 (53%), Positives = 133/181 (73%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
RNP VM K+RDEL ++IGE VRESHL L YL+AC+KE+ A
Sbjct: 297 RNPDVMYKLRDELTKIIGES-----PVRESHLPHLPYLQACVKETLRLHPPAPLLLPHRA 351
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
++TC+VM YT+PK +V VN+WA+GRDPKVW+ PL+F+PERFL+S +++KGNDFE+ PFG
Sbjct: 352 METCQVMGYTIPKDSQVFVNIWAMGRDPKVWDXPLSFTPERFLDSKLEFKGNDFEYIPFG 411
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
GRRICPG+ LG+R V L++A+L+H FDWSLPD M +++M+E +TL +++PL L+P
Sbjct: 412 XGRRICPGMALGARQVPLVLATLVHLFDWSLPDNMDSAQIDMEEWLVITLRKENPLRLVP 471
Query: 168 K 166
K
Sbjct: 472 K 472
>ref|XP_002509713.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
communis] gi|223549612|gb|EEF51100.1|
(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
[Ricinus communis]
Length = 501
Score = 211 bits (538), Expect = 3e-52
Identities = 101/181 (55%), Positives = 131/181 (72%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
+NPK M+KV++E+ R ++ES ++ LTYL+AC+KE+ A
Sbjct: 316 KNPKCMKKVQEEIT-----RNLIPDILKESPISNLTYLQACVKETLRLHPPGPFLLPHRA 370
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
DTC+VM+YT+PK +V+VN WAIGRDPK W+DPL F PERFLNS++DYKGNDFEF PFG
Sbjct: 371 TDTCQVMNYTIPKNSQVLVNFWAIGRDPKYWKDPLIFKPERFLNSNLDYKGNDFEFIPFG 430
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
+GRRICPGLP+ ++ V LI+ASLIH FDWSLP G +L+M E++GLTL + PLLLIP
Sbjct: 431 SGRRICPGLPMAAKQVPLIVASLIHFFDWSLPGGKDSIDLDMTEKYGLTLRMEKPLLLIP 490
Query: 168 K 166
K
Sbjct: 491 K 491
>ref|XP_010112567.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 [Morus notabilis]
gi|587947837|gb|EXC34114.1| (S)-N-methylcoclaurine
3'-hydroxylase isozyme 1 [Morus notabilis]
Length = 515
Score = 211 bits (537), Expect = 3e-52
Identities = 98/181 (54%), Positives = 129/181 (71%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
RNPK M+ +R+ELE+ IG + V E L +L YL+ C+KE A
Sbjct: 336 RNPKCMKILREELEREIGSQE----MVMECDLPKLKYLECCLKEPLRLPPPAPLPIPHRA 391
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
+TC+VM+YTVPK ++VNVWAIGRDP WEDPL F PERFLNSS+D+KG +FE+ PFG
Sbjct: 392 AETCQVMNYTVPKNSEILVNVWAIGRDPNHWEDPLEFKPERFLNSSLDFKGTNFEYLPFG 451
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
AGRRICPG PL S+ V LI+ASL+H+FDWSLP+G P EL+M E++ + +++K PLL++P
Sbjct: 452 AGRRICPGQPLASKQVPLIVASLVHSFDWSLPNGKDPKELDMAEKYDINMLKKEPLLVVP 511
Query: 168 K 166
K
Sbjct: 512 K 512
>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 211 bits (537), Expect = 3e-52
Identities = 100/181 (55%), Positives = 128/181 (70%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
+ P V+ K+R EL V+G+ V+ES L L YL+A +KE+ R A
Sbjct: 307 KKPLVVSKIRLELVNVVGDN-----TVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRA 361
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
L+TC VM+YT+PK C++MVN WAIGRDPK W+DPL F PERFL+S VDYKGNDFE PFG
Sbjct: 362 LETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFG 421
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
GRRICPGLPL S+ + LI+A+L+ F+WSLP GM +EL+MDE+FGLTL + PLL++
Sbjct: 422 GGRRICPGLPLASQFISLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVL 481
Query: 168 K 166
K
Sbjct: 482 K 482
>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase
isozyme 2; AltName: Full=Cytochrome P450 80B2 [Coptis
japonica] gi|9971208|dbj|BAB12433.1|
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 211 bits (536), Expect = 4e-52
Identities = 102/186 (54%), Positives = 130/186 (69%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
+ P V+ K+R EL V+G+ V+ES L L YL+A +KE+ R A
Sbjct: 307 KKPLVVSKIRLELVNVVGDN-----TVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRA 361
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
L+TC VM+YT+PK C++MVN WAIGRDPK W+DPL F PERFL+S VDYKGNDFE PFG
Sbjct: 362 LETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFG 421
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
GRRICPGLPL S+ LI+A+L+ F+WSLP GM +EL+MDE+FGLTL + PLL++
Sbjct: 422 GGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVL 481
Query: 168 K*VATN 151
K A+N
Sbjct: 482 KARASN 487
>ref|XP_012087221.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Jatropha curcas] gi|643712075|gb|KDP25503.1|
hypothetical protein JCGZ_20659 [Jatropha curcas]
Length = 499
Score = 210 bits (535), Expect = 6e-52
Identities = 97/183 (53%), Positives = 132/183 (72%), Gaps = 2/183 (1%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
RNPK M+KV +E+++ +SHL + T+L+AC KE+ A
Sbjct: 319 RNPKYMKKVEEEIQRETNNEEN-----LDSHLHKFTFLQACFKETLRLHPPGPLLVPHRA 373
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
++C+VM+YT+PK +VMVN WAIGRDPK+WEDPL F PERFLNS++D+KGNDFE+ PF
Sbjct: 374 TESCQVMNYTIPKNSQVMVNFWAIGRDPKIWEDPLEFKPERFLNSNLDFKGNDFEYIPFS 433
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPN--ELNMDERFGLTLVRKHPLLL 175
+GRRICPGLP+ S+ V LI+A L+H FDWSLP+G N ++NM+E++GLTL+++ PLLL
Sbjct: 434 SGRRICPGLPMASKQVPLIVACLVHFFDWSLPNGQIRNAGQINMNEKYGLTLMKEQPLLL 493
Query: 174 IPK 166
IPK
Sbjct: 494 IPK 496
>ref|XP_011029198.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Populus euphratica]
Length = 507
Score = 209 bits (532), Expect = 1e-51
Identities = 95/180 (52%), Positives = 135/180 (75%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
+NP+ ++KV++E+ VI R+ + +ES+L +LTYL+AC+KE+ A
Sbjct: 326 KNPRCLKKVQEEIANVINMNRDTGF--KESYLPQLTYLQACVKETLRLHPPGPFLLPHRA 383
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
+D+C+VM+YT+PK +V+VN WAIGRDPK WE+P+ F+PERFL++++D+KGNDFEF PFG
Sbjct: 384 IDSCQVMNYTIPKNTQVLVNYWAIGRDPKSWEEPVVFNPERFLSANLDFKGNDFEFIPFG 443
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
+GRRICPGLP+ ++ V LIIA LI FDWSLP G P +L+M E +GLTL ++ PLLL+P
Sbjct: 444 SGRRICPGLPMAAKHVALIIAYLILFFDWSLPCGKNPTDLDMSENYGLTLRKEQPLLLVP 503
>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 209 bits (531), Expect = 2e-51
Identities = 96/181 (53%), Positives = 131/181 (72%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
+NP+V +R EL V+G+R V+ES + + YL+A +KE+ R A
Sbjct: 303 KNPQVTANMRLELLSVVGKR-----PVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRA 357
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
L+TC+V++YT+PK C++MVN W IGRDPK W DPL FSPERFLNSS+D+KGNDFE PFG
Sbjct: 358 LETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFG 417
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
AGRRICPG+PL ++ + LI++SL+ FDW LP GM P++L M+E+FGLTL ++ PL ++P
Sbjct: 418 AGRRICPGVPLATQFISLIVSSLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIVP 477
Query: 168 K 166
K
Sbjct: 478 K 478
>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1, partial
[Eschscholzia californica] gi|3127027|gb|AAC39452.1|
(S)-N-methylcoclaurine 3'-hydroxylase [Eschscholzia
californica]
Length = 487
Score = 208 bits (529), Expect = 3e-51
Identities = 92/181 (50%), Positives = 129/181 (71%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
+NPK+ K+R E++ V+GER +V+ES L YL+A +KE+ R A
Sbjct: 309 KNPKITAKIRSEIQTVVGER-----SVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRA 363
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
L+TC +++YT+PK C++MVN W IGRDPK W DPLTFSPERFLNSSVD++GNDF PFG
Sbjct: 364 LETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFSLIPFG 423
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
AGRRICPGLP+ ++ + L++A+ + DW LP+GM + L ++E+FGLTL ++ PL ++P
Sbjct: 424 AGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVP 483
Query: 168 K 166
K
Sbjct: 484 K 484
>ref|XP_011041604.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Populus euphratica]
Length = 492
Score = 207 bits (528), Expect = 4e-51
Identities = 94/181 (51%), Positives = 134/181 (74%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
+N +V++KV +E++ R +++ESH+++L YL AC+ E+ R A
Sbjct: 314 KNKEVLKKVSEEIK-----RETDTNSLKESHVSQLPYLNACVNETLRLHPPVPFLIPRRA 368
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
L+TC+VMDYT+P+ V+VNVWA+GRDP +WEDPL+F PERFL S +D+KG+DFEF PFG
Sbjct: 369 LETCKVMDYTIPRDSEVIVNVWAVGRDPSLWEDPLSFKPERFLGSDLDFKGHDFEFLPFG 428
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
AGRRICPGLP+ ++ V LIIA+L++ FDWSLP+G P L+M E+FG+TL ++ PLLL+P
Sbjct: 429 AGRRICPGLPMAAKQVHLIIATLLYYFDWSLPNGEDPATLDMSEKFGITLQKEQPLLLVP 488
Query: 168 K 166
+
Sbjct: 489 R 489
>gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
californica [Expression vector pWCD2353]
Length = 490
Score = 207 bits (527), Expect = 5e-51
Identities = 93/181 (51%), Positives = 129/181 (71%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
+NPK+ K+R EL+ V+GER +V+ES L YL+A +KE+ R A
Sbjct: 310 KNPKITAKLRSELQTVVGER-----SVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRA 364
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
L+TC +++YT+PK C++MVN W IGRDPK W DPLTFSPERFLNSSVD++GNDF PFG
Sbjct: 365 LETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLIPFG 424
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
AGRRICPGLP+ ++ + L++A+ + DW LP+GM + L ++E+FGLTL ++ PL ++P
Sbjct: 425 AGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVP 484
Query: 168 K 166
K
Sbjct: 485 K 485
>gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 207 bits (527), Expect = 5e-51
Identities = 95/181 (52%), Positives = 130/181 (71%)
Frame = -3
Query: 708 RNPKVMQKVRDELEQVIGERREGEYAVRESHLARLTYLKACIKESXXXXXXXXXXXXREA 529
+NP+V +R EL V+G+R V+ES + + YL+A +KE+ R A
Sbjct: 303 KNPQVTANMRLELLSVVGKR-----PVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRA 357
Query: 528 LDTCEVMDYTVPKGCRVMVNVWAIGRDPKVWEDPLTFSPERFLNSSVDYKGNDFEFTPFG 349
L+TC+V++YT+PK C++MVN W IGRDPK W DPL FSPERFLNSS+D+KGNDFE PFG
Sbjct: 358 LETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFG 417
Query: 348 AGRRICPGLPLGSRVVELIIASLIHTFDWSLPDGMQPNELNMDERFGLTLVRKHPLLLIP 169
AGRRICPG+PL ++ + LI++SL+ FDW P GM P++L M+E+FGLTL ++ PL ++P
Sbjct: 418 AGRRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIVP 477
Query: 168 K 166
K
Sbjct: 478 K 478