BLASTX nr result
ID: Cinnamomum24_contig00018844
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cinnamomum24_contig00018844
(319 letters)
Database: ./nr
69,698,275 sequences; 24,982,196,650 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_010249688.1| PREDICTED: nibrin isoform X2 [Nelumbo nucifera] 123 4e-26
ref|XP_010249680.1| PREDICTED: nibrin isoform X1 [Nelumbo nucifera] 123 4e-26
ref|XP_010918403.1| PREDICTED: nijmegen breakage syndrome 1 prot... 115 9e-24
ref|XP_010918402.1| PREDICTED: nijmegen breakage syndrome 1 prot... 115 9e-24
emb|CDP01718.1| unnamed protein product [Coffea canephora] 114 2e-23
ref|XP_009401854.1| PREDICTED: uncharacterized protein LOC103985... 114 2e-23
ref|XP_009401853.1| PREDICTED: uncharacterized protein LOC103985... 114 2e-23
ref|XP_011624625.1| PREDICTED: nijmegen breakage syndrome 1 prot... 113 6e-23
ref|XP_008787059.1| PREDICTED: uncharacterized protein LOC103705... 110 4e-22
ref|XP_008787056.1| PREDICTED: nibrin isoform X1 [Phoenix dactyl... 110 4e-22
ref|XP_003631844.1| PREDICTED: nijmegen breakage syndrome 1 prot... 108 1e-21
emb|CAN75336.1| hypothetical protein VITISV_012698 [Vitis vinifera] 108 1e-21
ref|XP_011096908.1| PREDICTED: nijmegen breakage syndrome 1 prot... 102 8e-20
ref|XP_010549887.1| PREDICTED: nibrin [Tarenaya hassleriana] 100 5e-19
ref|XP_007141986.1| hypothetical protein PHAVU_008G242800g [Phas... 100 5e-19
ref|XP_006363212.1| PREDICTED: nibrin-like [Solanum tuberosum] 100 6e-19
ref|XP_004232654.1| PREDICTED: nibrin isoform X2 [Solanum lycope... 100 6e-19
ref|XP_004232653.1| PREDICTED: nibrin isoform X1 [Solanum lycope... 100 6e-19
ref|XP_009782476.1| PREDICTED: nibrin isoform X2 [Nicotiana sylv... 99 8e-19
ref|XP_009782474.1| PREDICTED: nibrin isoform X1 [Nicotiana sylv... 99 8e-19
>ref|XP_010249688.1| PREDICTED: nibrin isoform X2 [Nelumbo nucifera]
Length = 490
Score = 123 bits (309), Expect = 4e-26
Identities = 59/98 (60%), Positives = 75/98 (76%)
Frame = -2
Query: 315 LIVRSTVSSSLNCSLKKNIVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCGSEVTEYV 136
+++ + +S++ K +VNFKCFRKRET SGNSFKDLIPFSK+P+ ESD GSEV EYV
Sbjct: 392 VVIDAKAPASVSSMTKTVVVNFKCFRKRETQSGNSFKDLIPFSKYPYNESDYGSEVAEYV 451
Query: 135 IKEKKRKQMEAVAEDLFNSEKVKRHGAARTSLRDLFTR 22
EKK+KQMEA+AEDLFN+EK +R G S+R+ TR
Sbjct: 452 KAEKKQKQMEAIAEDLFNNEKKRRRGPTGPSIREFLTR 489
>ref|XP_010249680.1| PREDICTED: nibrin isoform X1 [Nelumbo nucifera]
Length = 523
Score = 123 bits (309), Expect = 4e-26
Identities = 59/98 (60%), Positives = 75/98 (76%)
Frame = -2
Query: 315 LIVRSTVSSSLNCSLKKNIVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCGSEVTEYV 136
+++ + +S++ K +VNFKCFRKRET SGNSFKDLIPFSK+P+ ESD GSEV EYV
Sbjct: 425 VVIDAKAPASVSSMTKTVVVNFKCFRKRETQSGNSFKDLIPFSKYPYNESDYGSEVAEYV 484
Query: 135 IKEKKRKQMEAVAEDLFNSEKVKRHGAARTSLRDLFTR 22
EKK+KQMEA+AEDLFN+EK +R G S+R+ TR
Sbjct: 485 KAEKKQKQMEAIAEDLFNNEKKRRRGPTGPSIREFLTR 522
>ref|XP_010918403.1| PREDICTED: nijmegen breakage syndrome 1 protein isoform X2 [Elaeis
guineensis]
Length = 573
Score = 115 bits (289), Expect = 9e-24
Identities = 61/101 (60%), Positives = 77/101 (76%), Gaps = 1/101 (0%)
Frame = -2
Query: 318 DLIVRSTVSSSLNCSLKKNIVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCGS-EVTE 142
DLIV++ + S K +VNFKCFRKR T SGNSF++LIPFSK P+ ES CGS E +E
Sbjct: 473 DLIVKNIGTLIPVRSTTKKVVNFKCFRKRNTMSGNSFRNLIPFSKDPYLESGCGSNESSE 532
Query: 141 YVIKEKKRKQMEAVAEDLFNSEKVKRHGAARTSLRDLFTRR 19
Y+ +EKKRKQMEA+AEDLFN+EKV++ A TSL+ L +RR
Sbjct: 533 YMKEEKKRKQMEAIAEDLFNNEKVRKRATACTSLQALLSRR 573
>ref|XP_010918402.1| PREDICTED: nijmegen breakage syndrome 1 protein isoform X1 [Elaeis
guineensis]
Length = 613
Score = 115 bits (289), Expect = 9e-24
Identities = 61/101 (60%), Positives = 77/101 (76%), Gaps = 1/101 (0%)
Frame = -2
Query: 318 DLIVRSTVSSSLNCSLKKNIVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCGS-EVTE 142
DLIV++ + S K +VNFKCFRKR T SGNSF++LIPFSK P+ ES CGS E +E
Sbjct: 513 DLIVKNIGTLIPVRSTTKKVVNFKCFRKRNTMSGNSFRNLIPFSKDPYLESGCGSNESSE 572
Query: 141 YVIKEKKRKQMEAVAEDLFNSEKVKRHGAARTSLRDLFTRR 19
Y+ +EKKRKQMEA+AEDLFN+EKV++ A TSL+ L +RR
Sbjct: 573 YMKEEKKRKQMEAIAEDLFNNEKVRKRATACTSLQALLSRR 613
>emb|CDP01718.1| unnamed protein product [Coffea canephora]
Length = 569
Score = 114 bits (286), Expect = 2e-23
Identities = 61/96 (63%), Positives = 74/96 (77%), Gaps = 3/96 (3%)
Frame = -2
Query: 318 DLIVRSTVSSSLNCSLKKNIVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCGS-EVTE 142
DLIVR ++S+S++ S ++NFKCFRKRETPSGNSF LIPFSK P+KESD G+ EV E
Sbjct: 472 DLIVRDSLSASVHSSTNDAVINFKCFRKRETPSGNSFSSLIPFSKDPYKESDYGNEEVAE 531
Query: 141 YVIKEKKRKQMEAVAEDLFNSEKVKRHGAA--RTSL 40
Y+ +EKKRKQMEA+AEDLF E +R AA RT L
Sbjct: 532 YLKEEKKRKQMEALAEDLFKYEVGRRRNAAGSRTGL 567
>ref|XP_009401854.1| PREDICTED: uncharacterized protein LOC103985762 isoform X2 [Musa
acuminata subsp. malaccensis]
Length = 491
Score = 114 bits (285), Expect = 2e-23
Identities = 59/101 (58%), Positives = 75/101 (74%), Gaps = 1/101 (0%)
Frame = -2
Query: 318 DLIVRSTVSSSLNCSLKKNIVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCGSEVT-E 142
DLIVR+ ++ S + NFKCFRK+ET SGNSFKDLIPFSK P+ ESD GS+ T E
Sbjct: 391 DLIVRNISTTYSQRSTTDEVFNFKCFRKKETVSGNSFKDLIPFSKDPYNESDFGSKGTSE 450
Query: 141 YVIKEKKRKQMEAVAEDLFNSEKVKRHGAARTSLRDLFTRR 19
Y+ +EKKRKQ+EA+AEDLFN++KV++ AA SL LF +
Sbjct: 451 YMKEEKKRKQIEAIAEDLFNNDKVRKRAAAGASLYSLFNHK 491
>ref|XP_009401853.1| PREDICTED: uncharacterized protein LOC103985762 isoform X1 [Musa
acuminata subsp. malaccensis]
Length = 542
Score = 114 bits (285), Expect = 2e-23
Identities = 59/101 (58%), Positives = 75/101 (74%), Gaps = 1/101 (0%)
Frame = -2
Query: 318 DLIVRSTVSSSLNCSLKKNIVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCGSEVT-E 142
DLIVR+ ++ S + NFKCFRK+ET SGNSFKDLIPFSK P+ ESD GS+ T E
Sbjct: 442 DLIVRNISTTYSQRSTTDEVFNFKCFRKKETVSGNSFKDLIPFSKDPYNESDFGSKGTSE 501
Query: 141 YVIKEKKRKQMEAVAEDLFNSEKVKRHGAARTSLRDLFTRR 19
Y+ +EKKRKQ+EA+AEDLFN++KV++ AA SL LF +
Sbjct: 502 YMKEEKKRKQIEAIAEDLFNNDKVRKRAAAGASLYSLFNHK 542
>ref|XP_011624625.1| PREDICTED: nijmegen breakage syndrome 1 protein [Amborella
trichopoda]
Length = 535
Score = 113 bits (282), Expect = 6e-23
Identities = 59/94 (62%), Positives = 72/94 (76%), Gaps = 1/94 (1%)
Frame = -2
Query: 318 DLIVRSTVSSSLNCSLKKNIVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCGSEVTEY 139
DLIVR+ + +S +NFK FRKRE PSGNSF+DLIPFSK+P+KESD GSEV ++
Sbjct: 428 DLIVRNGLVTSNRNKSSSEAINFKRFRKREVPSGNSFRDLIPFSKYPYKESDYGSEVADF 487
Query: 138 VIKEKKRKQMEAVAEDLFNSEKVKR-HGAARTSL 40
+EKKRK+MEAVA+DLFNSEKVKR G +SL
Sbjct: 488 RREEKKRKEMEAVADDLFNSEKVKRQRGTTASSL 521
>ref|XP_008787059.1| PREDICTED: uncharacterized protein LOC103705199 isoform X2 [Phoenix
dactylifera]
Length = 520
Score = 110 bits (275), Expect = 4e-22
Identities = 57/101 (56%), Positives = 73/101 (72%), Gaps = 1/101 (0%)
Frame = -2
Query: 318 DLIVRSTVSSSLNCSLKKNIVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCGS-EVTE 142
DLIV++ + S + NFKCFRKR T SGNSF++LIPFSK P+ ES CGS E +E
Sbjct: 420 DLIVKNIGTPLPGRSTTDKVANFKCFRKRNTMSGNSFRNLIPFSKDPYLESGCGSNESSE 479
Query: 141 YVIKEKKRKQMEAVAEDLFNSEKVKRHGAARTSLRDLFTRR 19
Y +EKKRKQ+EA+AEDLFN+EK ++ A TSL+ L +RR
Sbjct: 480 YTREEKKRKQIEAIAEDLFNNEKARKRATAGTSLQALLSRR 520
>ref|XP_008787056.1| PREDICTED: nibrin isoform X1 [Phoenix dactylifera]
gi|672127190|ref|XP_008787057.1| PREDICTED: nibrin
isoform X1 [Phoenix dactylifera]
gi|672127192|ref|XP_008787058.1| PREDICTED: nibrin
isoform X1 [Phoenix dactylifera]
Length = 573
Score = 110 bits (275), Expect = 4e-22
Identities = 57/101 (56%), Positives = 73/101 (72%), Gaps = 1/101 (0%)
Frame = -2
Query: 318 DLIVRSTVSSSLNCSLKKNIVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCGS-EVTE 142
DLIV++ + S + NFKCFRKR T SGNSF++LIPFSK P+ ES CGS E +E
Sbjct: 473 DLIVKNIGTPLPGRSTTDKVANFKCFRKRNTMSGNSFRNLIPFSKDPYLESGCGSNESSE 532
Query: 141 YVIKEKKRKQMEAVAEDLFNSEKVKRHGAARTSLRDLFTRR 19
Y +EKKRKQ+EA+AEDLFN+EK ++ A TSL+ L +RR
Sbjct: 533 YTREEKKRKQIEAIAEDLFNNEKARKRATAGTSLQALLSRR 573
>ref|XP_003631844.1| PREDICTED: nijmegen breakage syndrome 1 protein [Vitis vinifera]
gi|731386389|ref|XP_010648864.1| PREDICTED: nijmegen
breakage syndrome 1 protein [Vitis vinifera]
gi|296083807|emb|CBI24024.3| unnamed protein product
[Vitis vinifera]
Length = 595
Score = 108 bits (271), Expect = 1e-21
Identities = 62/100 (62%), Positives = 73/100 (73%), Gaps = 1/100 (1%)
Frame = -2
Query: 318 DLIVRSTVSSSLNCSLKKNIVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCGS-EVTE 142
DLIVR + S K IVNFKCFRK +T SGNSF +LIPF+K+P+KESD GS EV +
Sbjct: 495 DLIVRDINLLTSFSSTKNRIVNFKCFRKAKTQSGNSFNNLIPFAKYPYKESDNGSDEVMQ 554
Query: 141 YVIKEKKRKQMEAVAEDLFNSEKVKRHGAARTSLRDLFTR 22
V +EKKRKQMEA+AEDLFN+EK +R G A SL L TR
Sbjct: 555 SVKEEKKRKQMEAIAEDLFNNEKGRRRGVA-GSLAGLLTR 593
>emb|CAN75336.1| hypothetical protein VITISV_012698 [Vitis vinifera]
Length = 280
Score = 108 bits (271), Expect = 1e-21
Identities = 62/100 (62%), Positives = 73/100 (73%), Gaps = 1/100 (1%)
Frame = -2
Query: 318 DLIVRSTVSSSLNCSLKKNIVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCGS-EVTE 142
DLIVR + S K IVNFKCFRK +T SGNSF +LIPF+K+P+KESD GS EV +
Sbjct: 180 DLIVRDINLLTSFSSTKNRIVNFKCFRKAKTQSGNSFNNLIPFAKYPYKESDNGSDEVMQ 239
Query: 141 YVIKEKKRKQMEAVAEDLFNSEKVKRHGAARTSLRDLFTR 22
V +EKKRKQMEA+AEDLFN+EK +R G A SL L TR
Sbjct: 240 SVKEEKKRKQMEAIAEDLFNNEKGRRRGVA-GSLAGLLTR 278
>ref|XP_011096908.1| PREDICTED: nijmegen breakage syndrome 1 protein [Sesamum indicum]
gi|747097858|ref|XP_011096909.1| PREDICTED: nijmegen
breakage syndrome 1 protein [Sesamum indicum]
gi|747097860|ref|XP_011096910.1| PREDICTED: nijmegen
breakage syndrome 1 protein [Sesamum indicum]
Length = 565
Score = 102 bits (255), Expect = 8e-20
Identities = 59/101 (58%), Positives = 74/101 (73%), Gaps = 2/101 (1%)
Frame = -2
Query: 318 DLIVRS-TVSSSLNCSLKKNIVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCGSE-VT 145
DLIVR + S SL +NFK FRK PSGNSF +LIPFSK+P++ESD G+E V
Sbjct: 465 DLIVREPNLPQSGPSSLNGAAINFKRFRKTSAPSGNSFNNLIPFSKYPYEESDYGNEDVA 524
Query: 144 EYVIKEKKRKQMEAVAEDLFNSEKVKRHGAARTSLRDLFTR 22
E V +EKKRKQMEA+A+DLFN+EK ++ GA+ SLR+LF R
Sbjct: 525 ESVKEEKKRKQMEAIADDLFNNEKGRKRGAS-GSLRELFAR 564
>ref|XP_010549887.1| PREDICTED: nibrin [Tarenaya hassleriana]
Length = 542
Score = 100 bits (248), Expect = 5e-19
Identities = 57/100 (57%), Positives = 70/100 (70%), Gaps = 1/100 (1%)
Frame = -2
Query: 318 DLIVRSTVSSSLNCSLKKN-IVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCGSEVTE 142
DLIVR T S S +VNFK FRK SGNSF +LIPFSK P+KE D G E+T+
Sbjct: 444 DLIVRDTQSIRTVLSAGDGGVVNFKRFRKGNITSGNSFSNLIPFSKDPYKEYDSG-EMTD 502
Query: 141 YVIKEKKRKQMEAVAEDLFNSEKVKRHGAARTSLRDLFTR 22
Y+ +EKKRKQMEA+AEDLFN+EK ++ G A S+R+ TR
Sbjct: 503 YIKEEKKRKQMEAIAEDLFNNEKARKRGTA-GSIREFLTR 541
>ref|XP_007141986.1| hypothetical protein PHAVU_008G242800g [Phaseolus vulgaris]
gi|561015119|gb|ESW13980.1| hypothetical protein
PHAVU_008G242800g [Phaseolus vulgaris]
Length = 557
Score = 100 bits (248), Expect = 5e-19
Identities = 49/88 (55%), Positives = 69/88 (78%), Gaps = 2/88 (2%)
Frame = -2
Query: 315 LIVRST-VSSSLNCSLKKNIVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCG-SEVTE 142
LIVR T + SS++ + + NFKCFRK T SGNSF +L+PF+K+P+K+SDCG E++E
Sbjct: 453 LIVRDTNIRSSVSTAPDSSFPNFKCFRKAHTQSGNSFDNLVPFAKYPYKDSDCGDDEMSE 512
Query: 141 YVIKEKKRKQMEAVAEDLFNSEKVKRHG 58
V +EK+RK+MEAVA+DLFN+EK ++ G
Sbjct: 513 LVKEEKRRKKMEAVADDLFNNEKTRKRG 540
>ref|XP_006363212.1| PREDICTED: nibrin-like [Solanum tuberosum]
Length = 586
Score = 99.8 bits (247), Expect = 6e-19
Identities = 57/102 (55%), Positives = 70/102 (68%), Gaps = 2/102 (1%)
Frame = -2
Query: 318 DLIVRSTVSS-SLNCSLKKNIVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCGSE-VT 145
DLIVR ++ S ++ S + NFK FRK PS NSF IPFSKHP++ES+C +E V
Sbjct: 486 DLIVRDSIMSLPVSSSSNGGVTNFKRFRKANAPSQNSFAIFIPFSKHPYQESECKNEDVA 545
Query: 144 EYVIKEKKRKQMEAVAEDLFNSEKVKRHGAARTSLRDLFTRR 19
E + +EKKRKQMEA+AEDLF SEK K+ G A SL LF RR
Sbjct: 546 ESIKEEKKRKQMEAIAEDLFKSEKGKKSGVA-GSLHGLFARR 586
>ref|XP_004232654.1| PREDICTED: nibrin isoform X2 [Solanum lycopersicum]
Length = 554
Score = 99.8 bits (247), Expect = 6e-19
Identities = 57/102 (55%), Positives = 70/102 (68%), Gaps = 2/102 (1%)
Frame = -2
Query: 318 DLIVRSTVSS-SLNCSLKKNIVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCGSE-VT 145
DLIVR ++ S ++ S + NFK FRK PS NSF IPFSKHP++ES+C +E V
Sbjct: 454 DLIVRDSIMSLPVSSSSNGGVANFKRFRKANAPSQNSFAIFIPFSKHPYQESECKNEDVA 513
Query: 144 EYVIKEKKRKQMEAVAEDLFNSEKVKRHGAARTSLRDLFTRR 19
E + +EKKRKQMEA+AEDLF SEK K+ G A SL LF RR
Sbjct: 514 ESIREEKKRKQMEAIAEDLFKSEKGKKSGVA-GSLHGLFARR 554
>ref|XP_004232653.1| PREDICTED: nibrin isoform X1 [Solanum lycopersicum]
gi|723673629|ref|XP_010316547.1| PREDICTED: nibrin
isoform X1 [Solanum lycopersicum]
Length = 563
Score = 99.8 bits (247), Expect = 6e-19
Identities = 57/102 (55%), Positives = 70/102 (68%), Gaps = 2/102 (1%)
Frame = -2
Query: 318 DLIVRSTVSS-SLNCSLKKNIVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCGSE-VT 145
DLIVR ++ S ++ S + NFK FRK PS NSF IPFSKHP++ES+C +E V
Sbjct: 463 DLIVRDSIMSLPVSSSSNGGVANFKRFRKANAPSQNSFAIFIPFSKHPYQESECKNEDVA 522
Query: 144 EYVIKEKKRKQMEAVAEDLFNSEKVKRHGAARTSLRDLFTRR 19
E + +EKKRKQMEA+AEDLF SEK K+ G A SL LF RR
Sbjct: 523 ESIREEKKRKQMEAIAEDLFKSEKGKKSGVA-GSLHGLFARR 563
>ref|XP_009782476.1| PREDICTED: nibrin isoform X2 [Nicotiana sylvestris]
Length = 537
Score = 99.4 bits (246), Expect = 8e-19
Identities = 55/101 (54%), Positives = 71/101 (70%), Gaps = 1/101 (0%)
Frame = -2
Query: 318 DLIVRSTVSSSLNCSLKKNIVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCGSE-VTE 142
DLIVR ++ SS S+ + NFK FRK PS NSF+ IPF+K+P++ES+ +E V E
Sbjct: 438 DLIVRDSIMSSPVSSINGGVTNFKRFRKTSAPSPNSFEIFIPFAKYPYQESEYKNEDVAE 497
Query: 141 YVIKEKKRKQMEAVAEDLFNSEKVKRHGAARTSLRDLFTRR 19
+ +EKKRKQMEA+AEDLFNSEK +R G A SL LF R+
Sbjct: 498 SIKEEKKRKQMEAIAEDLFNSEKGRRRGTA-GSLHGLFARK 537
>ref|XP_009782474.1| PREDICTED: nibrin isoform X1 [Nicotiana sylvestris]
gi|698464594|ref|XP_009782475.1| PREDICTED: nibrin
isoform X1 [Nicotiana sylvestris]
Length = 555
Score = 99.4 bits (246), Expect = 8e-19
Identities = 55/101 (54%), Positives = 71/101 (70%), Gaps = 1/101 (0%)
Frame = -2
Query: 318 DLIVRSTVSSSLNCSLKKNIVNFKCFRKRETPSGNSFKDLIPFSKHPHKESDCGSE-VTE 142
DLIVR ++ SS S+ + NFK FRK PS NSF+ IPF+K+P++ES+ +E V E
Sbjct: 456 DLIVRDSIMSSPVSSINGGVTNFKRFRKTSAPSPNSFEIFIPFAKYPYQESEYKNEDVAE 515
Query: 141 YVIKEKKRKQMEAVAEDLFNSEKVKRHGAARTSLRDLFTRR 19
+ +EKKRKQMEA+AEDLFNSEK +R G A SL LF R+
Sbjct: 516 SIKEEKKRKQMEAIAEDLFNSEKGRRRGTA-GSLHGLFARK 555