BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00027292
         (433 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI16383.3| unnamed protein product [Vitis vinifera]              114   8e-24
ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnm...   114   8e-24
ref|XP_002894309.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m...   105   3e-21
ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   104   8e-21
ref|NP_175542.2| tRNA (5-methylaminomethyl-2-thiouridylate)-meth...   102   4e-20

>emb|CBI16383.3| unnamed protein product [Vitis vinifera]
          Length = 544

 Score =  114 bits (285), Expect = 8e-24
 Identities = 66/127 (51%), Positives = 80/127 (62%), Gaps = 1/127 (0%)
 Frame = +1

Query: 55  SPRNPNTLKYYS-FSAFPLKTLIKPSTPKTLSKSFSLSSIKTPNFPVRSISTGEAIELEP 231
           S RNPN     S FS  P      P+    L  S + +++   +      +T   I   P
Sbjct: 15  SLRNPNLRFCISLFSPKPFSLCFLPN----LRPSITTAAVAATSAAASGTTT---ITTPP 67

Query: 232 NSLSNGETKELGPYLTCSMPGKRLKVAVLLSGGVDSSVALRLLHLAGHSCTAFYLKIWFQ 411
           ++L N +   L PYL+CS+P  RLK+AVLLSGGVDSSVALRLLH AGHSCTAFYLKIWFQ
Sbjct: 68  SNLFNAD---LQPYLSCSLPNNRLKIAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQ 124

Query: 412 EDYENFW 432
           ED+ENFW
Sbjct: 125 EDFENFW 131


>ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnmA-like [Vitis vinifera]
          Length = 540

 Score =  114 bits (285), Expect = 8e-24
 Identities = 66/127 (51%), Positives = 80/127 (62%), Gaps = 1/127 (0%)
 Frame = +1

Query: 55  SPRNPNTLKYYS-FSAFPLKTLIKPSTPKTLSKSFSLSSIKTPNFPVRSISTGEAIELEP 231
           S RNPN     S FS  P      P+    L  S + +++   +      +T   I   P
Sbjct: 11  SLRNPNLRFCISLFSPKPFSLCFLPN----LRPSITTAAVAATSAAASGTTT---ITTPP 63

Query: 232 NSLSNGETKELGPYLTCSMPGKRLKVAVLLSGGVDSSVALRLLHLAGHSCTAFYLKIWFQ 411
           ++L N +   L PYL+CS+P  RLK+AVLLSGGVDSSVALRLLH AGHSCTAFYLKIWFQ
Sbjct: 64  SNLFNAD---LQPYLSCSLPNNRLKIAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQ 120

Query: 412 EDYENFW 432
           ED+ENFW
Sbjct: 121 EDFENFW 127


>ref|XP_002894309.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
           [Arabidopsis lyrata subsp. lyrata]
           gi|297340151|gb|EFH70568.1| tRNA
           (5-methylaminomethyl-2-thiouridylate)-methyltransferase
           [Arabidopsis lyrata subsp. lyrata]
          Length = 500

 Score =  105 bits (263), Expect = 3e-21
 Identities = 64/122 (52%), Positives = 75/122 (61%), Gaps = 14/122 (11%)
 Frame = +1

Query: 109 KTLIKPSTP--KTLSKSFSLSSIKTPNFPVRSISTGEAIELEPNS-------LSNGETKE 261
           K  + PS P  +    S S+S    P+F   S S+  ++   P S       LS+G    
Sbjct: 7   KPFLSPSPPLFRLPLSSISISFRVFPSFFSSSPSSSSSLLPSPQSSDPITPLLSSGLNYS 66

Query: 262 -----LGPYLTCSMPGKRLKVAVLLSGGVDSSVALRLLHLAGHSCTAFYLKIWFQEDYEN 426
                L  YL+CSMP K L+VAVLLSGGVDSSVALRLLH AGHSCTAFYLKIWFQE +EN
Sbjct: 67  RHAFLLDQYLSCSMPEKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFEN 126

Query: 427 FW 432
           FW
Sbjct: 127 FW 128


>ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Cucumis
           sativus] gi|449524848|ref|XP_004169433.1| PREDICTED:
           tRNA-specific 2-thiouridylase MnmA-like [Cucumis
           sativus]
          Length = 538

 Score =  104 bits (259), Expect = 8e-21
 Identities = 48/55 (87%), Positives = 51/55 (92%)
 Frame = +1

Query: 268 PYLTCSMPGKRLKVAVLLSGGVDSSVALRLLHLAGHSCTAFYLKIWFQEDYENFW 432
           P+L+CSMP   LKVAVLLSGGVDSSVALRLLH AGHSCTAFYLKIWFQED+ENFW
Sbjct: 71  PFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFW 125


>ref|NP_175542.2| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
           [Arabidopsis thaliana] gi|332194528|gb|AEE32649.1| tRNA
           (5-methylaminomethyl-2-thiouridylate)-methyltransferase
           [Arabidopsis thaliana]
          Length = 497

 Score =  102 bits (253), Expect = 4e-20
 Identities = 47/54 (87%), Positives = 50/54 (92%)
 Frame = +1

Query: 271 YLTCSMPGKRLKVAVLLSGGVDSSVALRLLHLAGHSCTAFYLKIWFQEDYENFW 432
           YL+CSMP K L+VAVLLSGGVDSSVALRLLH AGHSCTAFYLKIWFQE +ENFW
Sbjct: 72  YLSCSMPEKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFW 125