BLASTX nr result
ID: Cimicifuga21_contig00016493
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cimicifuga21_contig00016493
(1790 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002281488.1| PREDICTED: tRNA (guanine-N(1)-)-methyltransf... 466 e-170
emb|CBI28113.3| unnamed protein product [Vitis vinifera] 457 e-168
sp|F6HH45.1|TRM52_VITVI RecName: Full=tRNA (guanine(37)-N1)-meth... 457 e-168
ref|XP_004139336.1| PREDICTED: tRNA (guanine(37)-N1)-methyltrans... 442 e-121
ref|XP_003549801.1| PREDICTED: LOW QUALITY PROTEIN: tRNA (guanin... 442 e-121
>ref|XP_002281488.1| PREDICTED: tRNA (guanine-N(1)-)-methyltransferase, mitochondrial-like
[Vitis vinifera]
Length = 506
Score = 466 bits (1199), Expect(2) = e-170
Identities = 233/361 (64%), Positives = 279/361 (77%), Gaps = 15/361 (4%)
Frame = +1
Query: 211 MFEESEFDLKLQLWALRIPRELSTAVTRILNGYMLDKARIKPIAEDPSCEKNRYVVLSDK 390
+ +ES+FDL+L+LWALRIP +L +RILNGY+LDK R+KPI EDP+CE NRY++LS++
Sbjct: 2 LLDESKFDLQLKLWALRIPSQLCKVASRILNGYLLDKPRVKPITEDPTCETNRYIILSER 61
Query: 391 IQNPDLSDIPDQKLCALKKLCKFEVVPYSLTLGYSYWGADHILKQILPPGVETPTSFETI 570
I+NPDLSDIP+ K LK LCK EVVPYSLTLGYSYW ADH+LKQILP GVE P+SFETI
Sbjct: 62 IKNPDLSDIPEDKRDELKVLCKIEVVPYSLTLGYSYWSADHVLKQILPDGVEVPSSFETI 121
Query: 571 GHIAHLNIQDELLSFKNVIAKVIYDKNQPKIRSVVNKVGTITNEFRVPKFELLAGIDDMV 750
GHIAHLNI ELL +K+VIAKVIYDKN P+I++VVNKVGTITNEFRVPKFE+L G DDMV
Sbjct: 122 GHIAHLNITGELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNEFRVPKFEILVGKDDMV 181
Query: 751 TEVKQNGAMFKLDYGLVYWNSRLETEHMRLVSLFQDGEVICDMFAGIGPFAIPAAQKGCA 930
TEVKQ A FKLDY LVYWNSRLE EHMRLVS F+ G++ICDMF+G+GPFAIPAAQKGC
Sbjct: 182 TEVKQYRATFKLDYSLVYWNSRLEHEHMRLVSQFRPGQIICDMFSGVGPFAIPAAQKGCL 241
Query: 931 VYANDLNPDSVHYLKINAEINEADDRISAYNMDARAFMSHLMQVP-------------EC 1071
VYANDLNPDS+ YLKINA+IN+ DD I AYNMDAR F+S LM+VP +
Sbjct: 242 VYANDLNPDSIRYLKINAKINKVDDNIWAYNMDARKFISQLMEVPVHEILPEPDVPVLKA 301
Query: 1072 VEIECNYPVNGDTFPESERPTVNMEEIPINGSSHQEGTKSPCTTSFS--AAAKRHSETCE 1245
E EC+ N T ++ TV + + SS+ +G + CT + AA K+HS +CE
Sbjct: 302 TE-ECSIQANVQTESQNGGLTVEAIGVHSDDSSNMDGIEGSCTVADDTVAAVKKHSGSCE 360
Query: 1246 E 1248
E
Sbjct: 361 E 361
Score = 161 bits (408), Expect(2) = e-170
Identities = 81/102 (79%), Positives = 87/102 (85%)
Frame = +2
Query: 1370 SNNIKTWEHVDHVIMNLPASALQFLDAFRGLICKKYWKGSLPWIHCYGFMRLTETKESII 1549
S+NIK WEHVDHVIMNLPASA+QFLDAFRGLI +KYWKGSLPWIHCY F+R ET E II
Sbjct: 391 SSNIKPWEHVDHVIMNLPASAIQFLDAFRGLIQRKYWKGSLPWIHCYCFIRANETNEMII 450
Query: 1550 SEAEKALNAKIKEPIHLHRVRRNVAPKKAMFCLSFILPEETC 1675
SEAE ALNA I+EPI LHRV R+VAP KAMFCLSF L EE C
Sbjct: 451 SEAETALNASIQEPI-LHRV-RDVAPNKAMFCLSFRLSEEAC 490
>emb|CBI28113.3| unnamed protein product [Vitis vinifera]
Length = 983
Score = 457 bits (1176), Expect(2) = e-168
Identities = 232/365 (63%), Positives = 278/365 (76%), Gaps = 19/365 (5%)
Frame = +1
Query: 211 MFEESEFDLKLQLWALRIPRELSTAVTRILNGYMLDKARIKPIAEDPSCEKNRYVVLSDK 390
+ +ES+FDL+L+LWALRIP +L +RILNGY+LDK R+KPI EDP+CE NRY++LS++
Sbjct: 2 LLDESKFDLQLKLWALRIPSQLCKVASRILNGYLLDKPRVKPITEDPTCETNRYIILSER 61
Query: 391 IQNPDLSDIPDQKLCALKKLCKFEVVPYSLTLGYSYWGADHILKQILPPGVETPTSFETI 570
I+NPDLSDIP+ K LK LCK EVVPYSLTLGYSYW ADH+LKQILP GVE P+SFETI
Sbjct: 62 IKNPDLSDIPEDKRDELKVLCKIEVVPYSLTLGYSYWSADHVLKQILPDGVEVPSSFETI 121
Query: 571 G----HIAHLNIQDELLSFKNVIAKVIYDKNQPKIRSVVNKVGTITNEFRVPKFELLAGI 738
HIAHLNI ELL +K+VIAKVIYDKN P+I++VVNKVGTITNEFRVPKFE+L G
Sbjct: 122 TFSLCHIAHLNITGELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNEFRVPKFEILVGK 181
Query: 739 DDMVTEVKQNGAMFKLDYGLVYWNSRLETEHMRLVSLFQDGEVICDMFAGIGPFAIPAAQ 918
DDMVTEVKQ A FKLDY LVYWNSRLE EHMRLVS F+ G++ICDMF+G+GPFAIPAAQ
Sbjct: 182 DDMVTEVKQYRATFKLDYSLVYWNSRLEHEHMRLVSQFRPGQIICDMFSGVGPFAIPAAQ 241
Query: 919 KGCAVYANDLNPDSVHYLKINAEINEADDRISAYNMDARAFMSHLMQVP----------- 1065
KGC VYANDLNPDS+ YLKINA+IN+ DD I AYNMDAR F+S LM+VP
Sbjct: 242 KGCLVYANDLNPDSIRYLKINAKINKVDDNIWAYNMDARKFISQLMEVPVHEILPEPDVP 301
Query: 1066 --ECVEIECNYPVNGDTFPESERPTVNMEEIPINGSSHQEGTKSPCTTSFS--AAAKRHS 1233
+ E EC+ N T ++ TV + + SS+ +G + CT + AA K+HS
Sbjct: 302 VLKATE-ECSIQANVQTESQNGGLTVEAIGVHSDDSSNMDGIEGSCTVADDTVAAVKKHS 360
Query: 1234 ETCEE 1248
+CEE
Sbjct: 361 GSCEE 365
Score = 161 bits (408), Expect(2) = e-168
Identities = 81/102 (79%), Positives = 87/102 (85%)
Frame = +2
Query: 1370 SNNIKTWEHVDHVIMNLPASALQFLDAFRGLICKKYWKGSLPWIHCYGFMRLTETKESII 1549
S+NIK WEHVDHVIMNLPASA+QFLDAFRGLI +KYWKGSLPWIHCY F+R ET E II
Sbjct: 395 SSNIKPWEHVDHVIMNLPASAIQFLDAFRGLIQRKYWKGSLPWIHCYCFIRANETNEMII 454
Query: 1550 SEAEKALNAKIKEPIHLHRVRRNVAPKKAMFCLSFILPEETC 1675
SEAE ALNA I+EPI LHRV R+VAP KAMFCLSF L EE C
Sbjct: 455 SEAETALNASIQEPI-LHRV-RDVAPNKAMFCLSFRLSEEAC 494
>sp|F6HH45.1|TRM52_VITVI RecName: Full=tRNA (guanine(37)-N1)-methyltransferase 2; AltName:
Full=M1G-methyltransferase 2; AltName: Full=tRNA [GM37]
methyltransferase 2; AltName: Full=tRNA methyltransferase
5 homolog 2
Length = 510
Score = 457 bits (1176), Expect(2) = e-168
Identities = 232/365 (63%), Positives = 278/365 (76%), Gaps = 19/365 (5%)
Frame = +1
Query: 211 MFEESEFDLKLQLWALRIPRELSTAVTRILNGYMLDKARIKPIAEDPSCEKNRYVVLSDK 390
+ +ES+FDL+L+LWALRIP +L +RILNGY+LDK R+KPI EDP+CE NRY++LS++
Sbjct: 2 LLDESKFDLQLKLWALRIPSQLCKVASRILNGYLLDKPRVKPITEDPTCETNRYIILSER 61
Query: 391 IQNPDLSDIPDQKLCALKKLCKFEVVPYSLTLGYSYWGADHILKQILPPGVETPTSFETI 570
I+NPDLSDIP+ K LK LCK EVVPYSLTLGYSYW ADH+LKQILP GVE P+SFETI
Sbjct: 62 IKNPDLSDIPEDKRDELKVLCKIEVVPYSLTLGYSYWSADHVLKQILPDGVEVPSSFETI 121
Query: 571 G----HIAHLNIQDELLSFKNVIAKVIYDKNQPKIRSVVNKVGTITNEFRVPKFELLAGI 738
HIAHLNI ELL +K+VIAKVIYDKN P+I++VVNKVGTITNEFRVPKFE+L G
Sbjct: 122 TFSLCHIAHLNITGELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNEFRVPKFEILVGK 181
Query: 739 DDMVTEVKQNGAMFKLDYGLVYWNSRLETEHMRLVSLFQDGEVICDMFAGIGPFAIPAAQ 918
DDMVTEVKQ A FKLDY LVYWNSRLE EHMRLVS F+ G++ICDMF+G+GPFAIPAAQ
Sbjct: 182 DDMVTEVKQYRATFKLDYSLVYWNSRLEHEHMRLVSQFRPGQIICDMFSGVGPFAIPAAQ 241
Query: 919 KGCAVYANDLNPDSVHYLKINAEINEADDRISAYNMDARAFMSHLMQVP----------- 1065
KGC VYANDLNPDS+ YLKINA+IN+ DD I AYNMDAR F+S LM+VP
Sbjct: 242 KGCLVYANDLNPDSIRYLKINAKINKVDDNIWAYNMDARKFISQLMEVPVHEILPEPDVP 301
Query: 1066 --ECVEIECNYPVNGDTFPESERPTVNMEEIPINGSSHQEGTKSPCTTSFS--AAAKRHS 1233
+ E EC+ N T ++ TV + + SS+ +G + CT + AA K+HS
Sbjct: 302 VLKATE-ECSIQANVQTESQNGGLTVEAIGVHSDDSSNMDGIEGSCTVADDTVAAVKKHS 360
Query: 1234 ETCEE 1248
+CEE
Sbjct: 361 GSCEE 365
Score = 161 bits (408), Expect(2) = e-168
Identities = 81/102 (79%), Positives = 87/102 (85%)
Frame = +2
Query: 1370 SNNIKTWEHVDHVIMNLPASALQFLDAFRGLICKKYWKGSLPWIHCYGFMRLTETKESII 1549
S+NIK WEHVDHVIMNLPASA+QFLDAFRGLI +KYWKGSLPWIHCY F+R ET E II
Sbjct: 395 SSNIKPWEHVDHVIMNLPASAIQFLDAFRGLIQRKYWKGSLPWIHCYCFIRANETNEMII 454
Query: 1550 SEAEKALNAKIKEPIHLHRVRRNVAPKKAMFCLSFILPEETC 1675
SEAE ALNA I+EPI LHRV R+VAP KAMFCLSF L EE C
Sbjct: 455 SEAETALNASIQEPI-LHRV-RDVAPNKAMFCLSFRLSEEAC 494
>ref|XP_004139336.1| PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2-like [Cucumis
sativus] gi|449518119|ref|XP_004166091.1| PREDICTED: tRNA
(guanine(37)-N1)-methyltransferase 2-like [Cucumis
sativus]
Length = 497
Score = 442 bits (1136), Expect = e-121
Identities = 229/364 (62%), Positives = 272/364 (74%), Gaps = 19/364 (5%)
Frame = +1
Query: 211 MFEESEFDLKLQLWALRIPRELSTAVTRILNGYMLDKARIKPIAEDPSCEKNRYVVLSDK 390
M +ES+FD+ L LWALRIPREL A +ILNGY++DK RIKP+ EDP+C+KNRYV+LS+K
Sbjct: 1 MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
Query: 391 IQNPDLSDIPDQKLCALKKLCKFEVVPYSLTLGYSYWGADHILKQILPPGVETPTSFETI 570
+Q P+LS+IP+ KL LK LC FE VPYSLTLGYSYWGADHILK+ILPP VE P+SFETI
Sbjct: 61 VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
Query: 571 G------HIAHLNIQDELLSFKNVIAKVIYDKNQPKIRSVVNKVGTITNEFRVPKFELLA 732
H+AHLNI DELL +K+VIAKVIYDKN P+I++VVNKVG+ITNEFRVPKFE+L
Sbjct: 121 VKYSNPCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILK 180
Query: 733 GIDDMVTEVKQNGAMFKLDYGLVYWNSRLETEHMRLVSLFQDGEVICDMFAGIGPFAIPA 912
G +DMVTEVKQ GA FKLDY LVYWNSRLE EH+RLVSLFQ GEVICDMFAGIGPFAIPA
Sbjct: 181 GENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPA 240
Query: 913 AQKGCAVYANDLNPDSVHYLKINAEINEADDRISAYNMDARAFMSHLMQVP--------- 1065
AQK C VYANDLNPDS+ YLK+NAEIN+ R+ YN+DAR F+S LM VP
Sbjct: 241 AQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESV 300
Query: 1066 ECVEIECNYPVNGDTFPESERPTVNMEE----IPINGSSHQEGTKSPCTTSFSAAAKRHS 1233
+ C + G T ES V ++E I I+ S ++ + + A KR S
Sbjct: 301 TSILKACEKGIGG-TNQESPLANVQVQEVQDYIKISNKSVEQSRSADISV---AVLKRSS 356
Query: 1234 ETCE 1245
E+CE
Sbjct: 357 ESCE 360
Score = 153 bits (386), Expect = 2e-34
Identities = 77/108 (71%), Positives = 89/108 (82%)
Frame = +2
Query: 1373 NNIKTWEHVDHVIMNLPASALQFLDAFRGLICKKYWKGSLPWIHCYGFMRLTETKESIIS 1552
++ +TWEHVDHVIMNLPASAL FLDAFRG I KKYWKGSLPWIHCY FMR E ++ IIS
Sbjct: 393 SHARTWEHVDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIIS 452
Query: 1553 EAEKALNAKIKEPIHLHRVRRNVAPKKAMFCLSFILPEETCVDQKPSS 1696
EAE ALNA I++PI HRV R+VAP KAM+CLSF LPE+ CVD+ +S
Sbjct: 453 EAESALNANIQDPI-FHRV-RDVAPNKAMYCLSFRLPEQ-CVDEDTTS 497
>ref|XP_003549801.1| PREDICTED: LOW QUALITY PROTEIN: tRNA
(guanine-N(1)-)-methyltransferase-like [Glycine max]
Length = 472
Score = 442 bits (1136), Expect = e-121
Identities = 217/325 (66%), Positives = 263/325 (80%), Gaps = 8/325 (2%)
Frame = +1
Query: 211 MFEESEFDLKLQLWALRIPRELSTAVTRILNGYMLDKARIKPIAEDPSCEKNRYVVLSDK 390
M +ES+F++ L+LWALRIP + TRILNGY+LDK R+KPIAEDP+C+KNRY++LSD
Sbjct: 1 MLDESKFEVHLKLWALRIPCQHCKLATRILNGYLLDKPRVKPIAEDPTCDKNRYLILSDN 60
Query: 391 IQNPDLSDIPDQKLCALKKLCKFEVVPYSLTLGYSYWGADHILKQILPPGVETPTSFETI 570
+ N DLSDIP QKL LK LC+ E+VPYSLTLGYS+W ADH+LKQILP GVE P+SFETI
Sbjct: 61 VHNQDLSDIPKQKLDELKGLCEIEIVPYSLTLGYSHWSADHVLKQILPTGVEVPSSFETI 120
Query: 571 GHIAHLNIQDELLSFKNVIAKVIYDKNQPKIRSVVNKVGTITNEFRVPKFELLAGIDDMV 750
G IAHLN+ DELL +K+VIAKVIYDKN P+I+++VNKVGTITNEFRVP+FE+LAG +M+
Sbjct: 121 GQIAHLNLHDELLPYKDVIAKVIYDKNYPRIKTIVNKVGTITNEFRVPEFEILAGEHNMI 180
Query: 751 TEVKQNGAMFKLDYGLVYWNSRLETEHMRLVSLFQDGEVICDMFAGIGPFAIPAAQKGCA 930
TEVKQ GA F+LDY LVYWNSRLE EH RLVS+FQ G+ ICDMF GIGPFAIPAAQKGC
Sbjct: 181 TEVKQYGATFRLDYRLVYWNSRLEHEHKRLVSMFQAGQTICDMFTGIGPFAIPAAQKGCI 240
Query: 931 VYANDLNPDSVHYLKINAEINEADDRISAYNMDARAFMSHLMQVPEC-VEIECNYPV--- 1098
VYANDLNPDS+HYL+INA+IN+ DDRI AYNMDAR F+S +M+VP V +E ++ V
Sbjct: 241 VYANDLNPDSIHYLRINAKINKVDDRIYAYNMDARKFISQMMEVPNNEVTLETSHEVPIL 300
Query: 1099 ----NGDTFPESERPTVNMEEIPIN 1161
N ++ E+E T N+ I N
Sbjct: 301 DTRDNAESNSENELLTENICNIDNN 325
Score = 144 bits (362), Expect = 1e-31
Identities = 71/95 (74%), Positives = 80/95 (84%)
Frame = +2
Query: 1382 KTWEHVDHVIMNLPASALQFLDAFRGLICKKYWKGSLPWIHCYGFMRLTETKESIISEAE 1561
KTWEHVDHVIMNLPASA+QFLDAFRGLI KKYWKG LPWIHCY F+R TET E+II+ AE
Sbjct: 372 KTWEHVDHVIMNLPASAVQFLDAFRGLIQKKYWKGCLPWIHCYCFIRATETPETIIAVAE 431
Query: 1562 KALNAKIKEPIHLHRVRRNVAPKKAMFCLSFILPE 1666
ALN +I++ HRV R+VAP KAM+CLSF LPE
Sbjct: 432 SALNTRIQDST-FHRV-RDVAPNKAMYCLSFRLPE 464