BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00001311
         (1225 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002268942.2| PREDICTED: 2-isopropylmalate synthase 2, chl...   576   e-162
gb|AAW81741.1| Putative 2-isopropylmalate synthase [Brassica ole...   550   e-154
gb|ABA26446.1| 2-isopropylmalate synthase 1 [Brassica insularis]      549   e-154
gb|ACB59195.1| 2-isopropylmalate synthase [Brassica oleracea]         548   e-153
ref|XP_002530549.1| isopropylmalate synthase, putative [Ricinus ...   547   e-153

>ref|XP_002268942.2| PREDICTED: 2-isopropylmalate synthase 2, chloroplastic-like [Vitis
            vinifera]
          Length = 628

 Score =  576 bits (1484), Expect = e-162
 Identities = 298/393 (75%), Positives = 332/393 (84%), Gaps = 18/393 (4%)
 Frame = +1

Query: 67   MASQLLNPSFSPTIPTS----LNPTNPTRSPFFFSKRLI-----------PQYHHLPSKS 201
            MAS LLNP+FS T   +    LN  + + +P   SK ++           P  H L +K 
Sbjct: 1    MASILLNPTFSTTTAATTTSKLNHPSFSTTPISCSKGILKAPIPSTLFFKPPSHSLATKV 60

Query: 202  IKCSL---SHSRPEYIPNRISDPNYLRIFDTTLRDGEQSPGATMTSKEKLDVARQLSKLG 372
            +   L   S  RPEYIPNRI DPNY+RIFDTTLRDGEQSPGATMTSKEKLD+ARQL+KLG
Sbjct: 61   LSHRLPVRSALRPEYIPNRIDDPNYVRIFDTTLRDGEQSPGATMTSKEKLDIARQLAKLG 120

Query: 373  VDIIEAGFPASSKDDLEAVKIIAKEVGNAVDDDGHVPVICGLARCNRKDIDAAWEAVKYA 552
            VDIIEAGFPA+SKDDL+AVKIIAKEVGNAVD++GHVPVICGLARCNR DI  AWEAVKYA
Sbjct: 121  VDIIEAGFPAASKDDLDAVKIIAKEVGNAVDENGHVPVICGLARCNRNDISTAWEAVKYA 180

Query: 553  KRPRIHTFIATSEIHLKYKLRKSREEVIAIAKEMVSYARSLGCNDVEFSPEDAGRSDREY 732
            KRPRIHTFIATSEIH+KYKL+KS +EVI IA+EMV YARSLGC+DVEFSPEDAGRS+RE+
Sbjct: 181  KRPRIHTFIATSEIHMKYKLKKSEKEVIEIAREMVKYARSLGCDDVEFSPEDAGRSEREF 240

Query: 733  LYKILEEVIKAGATTLNIPDTVGYNCSFEFGKLIADIKSNTPGIDNVIISTHCQNDLGFA 912
            LYKIL EVIKAGATTLNIPDTVGY    EFG+LIADIKSNTPGI+NVIISTHCQNDLG +
Sbjct: 241  LYKILGEVIKAGATTLNIPDTVGYTVPNEFGQLIADIKSNTPGIENVIISTHCQNDLGLS 300

Query: 913  SSNTIEGVRAGARQVEVTINGIGERAGNAALEEVVMAIKCRGKELLGGLYTGVNTRHLNM 1092
            ++NTI G  AGARQVEVTINGIGERAGNA+LEEVVMA+KCRG++ LGGLYTG+N RH+  
Sbjct: 301  TANTIAGACAGARQVEVTINGIGERAGNASLEEVVMALKCRGEKALGGLYTGINIRHIVK 360

Query: 1093 TSKMVAEYSGLQVQPHKAIVGANAFAHESGIHQ 1191
            TSKMVAEYSGLQVQPHKAIVGANAFAHESGIHQ
Sbjct: 361  TSKMVAEYSGLQVQPHKAIVGANAFAHESGIHQ 393


>gb|AAW81741.1| Putative 2-isopropylmalate synthase [Brassica oleracea]
          Length = 623

 Score =  550 bits (1416), Expect = e-154
 Identities = 278/388 (71%), Positives = 325/388 (83%), Gaps = 13/388 (3%)
 Frame = +1

Query: 67   MASQLL-NPSFS-PTIPTSLNPTNPTRSPFFFSKRLIPQYHH----LPSKSIKCSLS--- 219
            MAS +L NP  S PT  T+ +  + +  P   S R  P +H     + S  + CSLS   
Sbjct: 1    MASSILRNPMLSSPTTITTPSLPSFSSKPSPLSFRFPPSHHRSSLRIKSLRLSCSLSDPS 60

Query: 220  ----HSRPEYIPNRISDPNYLRIFDTTLRDGEQSPGATMTSKEKLDVARQLSKLGVDIIE 387
                  RPEYIPNRISDPNY+R+FDTTLRDGEQSPGAT+TSKEKLD+ARQL+KLGVD+IE
Sbjct: 61   PPLRRCRPEYIPNRISDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDVIE 120

Query: 388  AGFPASSKDDLEAVKIIAKEVGNAVDDDGHVPVICGLARCNRKDIDAAWEAVKYAKRPRI 567
            AGFPA+SKDD EAVK IA+ VGNAVD DG+VPVICGL+RCN++DI+ AWEAVKYAKRPRI
Sbjct: 121  AGFPAASKDDFEAVKTIAETVGNAVDGDGYVPVICGLSRCNKRDIETAWEAVKYAKRPRI 180

Query: 568  HTFIATSEIHLKYKLRKSREEVIAIAKEMVSYARSLGCNDVEFSPEDAGRSDREYLYKIL 747
            HTFIATS+IHL+YKL+KS++EVI  A+ MV +ARSLGC DVEFSPEDAGRS+RE+LY+IL
Sbjct: 181  HTFIATSDIHLEYKLKKSKDEVIEFARNMVKFARSLGCEDVEFSPEDAGRSEREFLYQIL 240

Query: 748  EEVIKAGATTLNIPDTVGYNCSFEFGKLIADIKSNTPGIDNVIISTHCQNDLGFASSNTI 927
             EVIKAGATTLNIPDTVG     EFG+LIADIK+NTPGI+NVIISTHCQNDLG +++NT+
Sbjct: 241  GEVIKAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIENVIISTHCQNDLGLSTANTL 300

Query: 928  EGVRAGARQVEVTINGIGERAGNAALEEVVMAIKCRGKELLGGLYTGVNTRHLNMTSKMV 1107
             G  +GARQVEVTINGIGERAGNA+LEEVVMAIKCRG  +LGGLYTG++TRH+ MTSKMV
Sbjct: 301  SGAHSGARQVEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLYTGIDTRHIVMTSKMV 360

Query: 1108 AEYSGLQVQPHKAIVGANAFAHESGIHQ 1191
             +Y+G+Q QPHKAIVGANAFAHESGIHQ
Sbjct: 361  EDYTGMQTQPHKAIVGANAFAHESGIHQ 388


>gb|ABA26446.1| 2-isopropylmalate synthase 1 [Brassica insularis]
          Length = 623

 Score =  549 bits (1414), Expect = e-154
 Identities = 277/386 (71%), Positives = 323/386 (83%), Gaps = 12/386 (3%)
 Frame = +1

Query: 70   ASQLLNPSFS-PTIPTSLNPTNPTRSPFFFSKRLIPQYHH----LPSKSIKCSLS----- 219
            +S L NP  S PT  T+ +  + +  P   S R  P +H     + S  + CSLS     
Sbjct: 3    SSILRNPMLSSPTTITTPSLPSFSSKPSPLSFRFPPSHHRSSLRIKSLRLSCSLSDPSPP 62

Query: 220  --HSRPEYIPNRISDPNYLRIFDTTLRDGEQSPGATMTSKEKLDVARQLSKLGVDIIEAG 393
                RPEYIPNRISDPNY+R+FDTTLRDGEQSPGAT+TSKEKLD+ARQL+KLGVD+IEAG
Sbjct: 63   LCRRRPEYIPNRISDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDVIEAG 122

Query: 394  FPASSKDDLEAVKIIAKEVGNAVDDDGHVPVICGLARCNRKDIDAAWEAVKYAKRPRIHT 573
            FPA+SKDD EAVK IA  VGNAVD DG+VPVICGL+RCN++DI+ AWEAVKYAKRPRIHT
Sbjct: 123  FPAASKDDFEAVKTIAGTVGNAVDGDGYVPVICGLSRCNKRDIETAWEAVKYAKRPRIHT 182

Query: 574  FIATSEIHLKYKLRKSREEVIAIAKEMVSYARSLGCNDVEFSPEDAGRSDREYLYKILEE 753
            FIATS+IHL+YKL+KS++EVI IA+ MV +ARSLGC DVEFSPEDAGRS+RE+LY+IL E
Sbjct: 183  FIATSDIHLEYKLKKSKDEVIEIARNMVKFARSLGCEDVEFSPEDAGRSEREFLYQILGE 242

Query: 754  VIKAGATTLNIPDTVGYNCSFEFGKLIADIKSNTPGIDNVIISTHCQNDLGFASSNTIEG 933
            VIKAGATTLNIPDTVG     EFG+LIADIK+NTPGI+NVIISTHCQNDLG +++NT+ G
Sbjct: 243  VIKAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIENVIISTHCQNDLGLSTANTLSG 302

Query: 934  VRAGARQVEVTINGIGERAGNAALEEVVMAIKCRGKELLGGLYTGVNTRHLNMTSKMVAE 1113
              +GARQVEVTINGIGERAGNA+LEEVVMAIKCRG  +LGGLYTG++TRH+ MTSKMV +
Sbjct: 303  AHSGARQVEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLYTGIDTRHIVMTSKMVED 362

Query: 1114 YSGLQVQPHKAIVGANAFAHESGIHQ 1191
            Y+G+Q QPHKAIVGANAFAHESGIHQ
Sbjct: 363  YTGMQTQPHKAIVGANAFAHESGIHQ 388


>gb|ACB59195.1| 2-isopropylmalate synthase [Brassica oleracea]
          Length = 672

 Score =  548 bits (1411), Expect = e-153
 Identities = 279/397 (70%), Positives = 329/397 (82%), Gaps = 20/397 (5%)
 Frame = +1

Query: 61   SSMASQLL-NP--SFSPTIPTSLNPTNPTRSPFFFSKRLIPQYHHLPSKS-----IKCSL 216
            S MAS LL NP  S SP++PT  + T  T +P  F       +HH    S     + CS+
Sbjct: 42   SPMASSLLRNPTLSSSPSLPT-FSSTITTTTPLSFRFPPYSHHHHHRRSSSASLRLSCSI 100

Query: 217  S------------HSRPEYIPNRISDPNYLRIFDTTLRDGEQSPGATMTSKEKLDVARQL 360
            S              RP+YIPNRISDPNY+RIFDTTLRDGEQSPGAT+TSKEKLD+ARQL
Sbjct: 101  SDPPPPLPPHPLRRRRPDYIPNRISDPNYVRIFDTTLRDGEQSPGATLTSKEKLDIARQL 160

Query: 361  SKLGVDIIEAGFPASSKDDLEAVKIIAKEVGNAVDDDGHVPVICGLARCNRKDIDAAWEA 540
            +KLGVDIIEAGFPA+SKDD EAVK I++ VGNAVD++G+VPVICGL+RCN+KDI  AWEA
Sbjct: 161  AKLGVDIIEAGFPAASKDDFEAVKTISETVGNAVDENGYVPVICGLSRCNKKDIQTAWEA 220

Query: 541  VKYAKRPRIHTFIATSEIHLKYKLRKSREEVIAIAKEMVSYARSLGCNDVEFSPEDAGRS 720
            V+YAKRPRIHTFIATS+IHL+YKL+KS++EVI IA+ MV +ARSLGC+DVEFSPEDAGRS
Sbjct: 221  VRYAKRPRIHTFIATSDIHLEYKLKKSKQEVIEIARSMVRFARSLGCDDVEFSPEDAGRS 280

Query: 721  DREYLYKILEEVIKAGATTLNIPDTVGYNCSFEFGKLIADIKSNTPGIDNVIISTHCQND 900
            +RE+LY+IL EVIKAGATTLNIPDTVG     EFG+LIAD+K+NTPGI+NV+ISTHCQND
Sbjct: 281  EREFLYEILGEVIKAGATTLNIPDTVGITLPSEFGQLIADLKANTPGIENVVISTHCQND 340

Query: 901  LGFASSNTIEGVRAGARQVEVTINGIGERAGNAALEEVVMAIKCRGKELLGGLYTGVNTR 1080
            LG +++NT+ G  AGARQVEVTINGIGERAGNA+LEEVVMAIKCRG  +LGGL+TG++TR
Sbjct: 341  LGLSTANTLSGAHAGARQVEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTR 400

Query: 1081 HLNMTSKMVAEYSGLQVQPHKAIVGANAFAHESGIHQ 1191
            H+ MTSKMV +Y+G+Q QPHKAIVGANAFAHESGIHQ
Sbjct: 401  HIVMTSKMVEDYTGMQTQPHKAIVGANAFAHESGIHQ 437


>ref|XP_002530549.1| isopropylmalate synthase, putative [Ricinus communis]
            gi|223529911|gb|EEF31840.1| isopropylmalate synthase,
            putative [Ricinus communis]
          Length = 632

 Score =  547 bits (1410), Expect = e-153
 Identities = 280/381 (73%), Positives = 314/381 (82%), Gaps = 8/381 (2%)
 Frame = +1

Query: 73   SQLLNPSFSPTIPTSLNPTNPTRSPFF----FSKRLIPQYHHLPSKSIKCSLSHS----R 228
            SQ+ +    P I  SL    PTR  FF    FS           S +I CS S +    R
Sbjct: 15   SQITSKPSPPRISHSLLHFTPTRCRFFSNSKFSVSFPSNSRFKSSTAISCSSSAAPLSRR 74

Query: 229  PEYIPNRISDPNYLRIFDTTLRDGEQSPGATMTSKEKLDVARQLSKLGVDIIEAGFPASS 408
            PEYIP+ I DP+Y+RIFDTTLRDGEQSPGAT+TSKEKLD+ARQLSKLGVDIIEAGFPA+S
Sbjct: 75   PEYIPSHIPDPSYVRIFDTTLRDGEQSPGATLTSKEKLDIARQLSKLGVDIIEAGFPAAS 134

Query: 409  KDDLEAVKIIAKEVGNAVDDDGHVPVICGLARCNRKDIDAAWEAVKYAKRPRIHTFIATS 588
            KDD EAVK IAKEVGNAVD+DG+VPVICGL+RCN KDI  AWEAVKYAKRPRIHTFIATS
Sbjct: 135  KDDFEAVKTIAKEVGNAVDEDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPRIHTFIATS 194

Query: 589  EIHLKYKLRKSREEVIAIAKEMVSYARSLGCNDVEFSPEDAGRSDREYLYKILEEVIKAG 768
             IH++YKLRKS+EEV+ IA+ MV +ARSLGC+DVEFSPEDAGRSDRE+LY IL EVIKAG
Sbjct: 195  AIHMEYKLRKSKEEVVEIARSMVKFARSLGCDDVEFSPEDAGRSDREFLYHILGEVIKAG 254

Query: 769  ATTLNIPDTVGYNCSFEFGKLIADIKSNTPGIDNVIISTHCQNDLGFASSNTIEGVRAGA 948
            ATTLNIPDTVG     EFG+LIADIK+NTPGID VIISTHCQNDLG +++NT+ G  AGA
Sbjct: 255  ATTLNIPDTVGITLPSEFGQLIADIKANTPGIDKVIISTHCQNDLGLSTANTLAGAYAGA 314

Query: 949  RQVEVTINGIGERAGNAALEEVVMAIKCRGKELLGGLYTGVNTRHLNMTSKMVAEYSGLQ 1128
            RQVEVTINGIGERAGNA+LEEVVMAIKCRG+ +LGGLYTG+N R + M SKMV EY+GL 
Sbjct: 315  RQVEVTINGIGERAGNASLEEVVMAIKCRGEHILGGLYTGINPRQITMASKMVEEYTGLH 374

Query: 1129 VQPHKAIVGANAFAHESGIHQ 1191
            +QPHKAIVGANAFAHESGIHQ
Sbjct: 375  LQPHKAIVGANAFAHESGIHQ 395