BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cephaelis21_contig00037825
         (661 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFB70991.1| strictosidine beta-D-glucosidase, partial [Mitrag...   226   2e-57
pdb|4ATD|A Chain A, Crystal Structure Of Native Raucaffricine Gl...   221   1e-55
pdb|4A3Y|A Chain A, Crystal Structure Of Raucaffricine Glucosida...   221   1e-55
pdb|3U57|A Chain A, Structures Of Alkaloid Biosynthetic Glucosid...   220   2e-55
emb|CAC08209.2| beta-glucosidase [Cicer arietinum]                    201   8e-50

>gb|AFB70991.1| strictosidine beta-D-glucosidase, partial [Mitragyna speciosa]
          Length = 257

 Score =  226 bits (577), Expect = 2e-57
 Identities = 120/210 (57%), Positives = 143/210 (68%), Gaps = 8/210 (3%)
 Frame = +1

Query: 55  SFHQEKERIPCCKN**L----I*ITI**ML-GGRLSAGVNKEGVQYYNNLINELLANGIE 219
           S+HQ KE I   KN  L      I+   +L GG L+AGVNKEG+ YYNNLI+ELLANGIE
Sbjct: 26  SYHQYKEDIKILKNMGLDAYRFSISWSRVLPGGNLNAGVNKEGINYYNNLIDELLANGIE 85

Query: 220 PYVTLFHWDVPQALESEYGGFLDRRIVDDFREFADLCFWEFGDRVKNWITLNEPWTFAVL 399
           PYVTLFHWDVPQALE +YGGFL  +IVDDFRE+ +LCFWEFGDRVK+WITLNEPW+ +V 
Sbjct: 86  PYVTLFHWDVPQALEDKYGGFLSSQIVDDFREYVELCFWEFGDRVKHWITLNEPWSSSVG 145

Query: 400 XXXXXXXXXXXXVVNTTEHVKDEVPK--HRCHCPHADDACEDGNPGTEPYIVTHNLILAH 573
                         ++ +      P    RC    +     +GNPGTEPY+VTHN +LAH
Sbjct: 146 GYVNGTFAPGRGASSSEQENDHPAPALLSRCSPWQSQSISSNGNPGTEPYVVTHNQLLAH 205

Query: 574 GEAVKLYRENY-KCQGGKIGITLVSQWWEP 660
             AV+LY+ N+ K Q GKIGITLVSQW EP
Sbjct: 206 AAAVELYKTNFQKSQNGKIGITLVSQWMEP 235


>pdb|4ATD|A Chain A, Crystal Structure Of Native Raucaffricine Glucosidase
           gi|442570519|pdb|4ATD|B Chain B, Crystal Structure Of
           Native Raucaffricine Glucosidase gi|444302131|pdb|4ATL|A
           Chain A, Crystal Structure Of Raucaffricine Glucosidase
           In Complex With Glucose gi|444302132|pdb|4ATL|B Chain B,
           Crystal Structure Of Raucaffricine Glucosidase In
           Complex With Glucose
          Length = 513

 Score =  221 bits (563), Expect = 1e-55
 Identities = 107/177 (60%), Positives = 127/177 (71%), Gaps = 1/177 (0%)
 Frame = +1

Query: 133 GGRLSAGVNKEGVQYYNNLINELLANGIEPYVTLFHWDVPQALESEYGGFLDRRIVDDFR 312
           GGRLS GVNKEG+ YYNNLI+ LLANGI+P+VTLFHWDVPQALE EYGGFL  RIVDDF 
Sbjct: 105 GGRLSGGVNKEGINYYNNLIDGLLANGIKPFVTLFHWDVPQALEDEYGGFLSPRIVDDFC 164

Query: 313 EFADLCFWEFGDRVKNWITLNEPWTFAVLXXXXXXXXXXXXVVNTTEHVKDEVPKHRCHC 492
           E+A+LCFWEFGDRVK+W+TLNEPWTF+V                + EHV     +HRC  
Sbjct: 165 EYAELCFWEFGDRVKHWMTLNEPWTFSV-HGYATGLYAPGRGRTSPEHVNHPTVQHRCST 223

Query: 493 PHADDACEDGNPGTEPYIVTHNLILAHGEAVKLYRENY-KCQGGKIGITLVSQWWEP 660
                 C  GNPGTEPY VTH+L+LAH  AV+LY+  + + Q G+IGI+  +QW EP
Sbjct: 224 VAPQCICSTGNPGTEPYWVTHHLLLAHAAAVELYKNKFQRGQEGQIGISHATQWMEP 280


>pdb|4A3Y|A Chain A, Crystal Structure Of Raucaffricine Glucosidase From
           Ajmaline Biosynthesis Pathway gi|400977294|pdb|4A3Y|B
           Chain B, Crystal Structure Of Raucaffricine Glucosidase
           From Ajmaline Biosynthesis Pathway
           gi|6103585|gb|AAF03675.1|AF149311_1
           raucaffricine-O-beta-D-glucosidase [Rauvolfia
           serpentina]
          Length = 540

 Score =  221 bits (563), Expect = 1e-55
 Identities = 107/177 (60%), Positives = 127/177 (71%), Gaps = 1/177 (0%)
 Frame = +1

Query: 133 GGRLSAGVNKEGVQYYNNLINELLANGIEPYVTLFHWDVPQALESEYGGFLDRRIVDDFR 312
           GGRLS GVNKEG+ YYNNLI+ LLANGI+P+VTLFHWDVPQALE EYGGFL  RIVDDF 
Sbjct: 105 GGRLSGGVNKEGINYYNNLIDGLLANGIKPFVTLFHWDVPQALEDEYGGFLSPRIVDDFC 164

Query: 313 EFADLCFWEFGDRVKNWITLNEPWTFAVLXXXXXXXXXXXXVVNTTEHVKDEVPKHRCHC 492
           E+A+LCFWEFGDRVK+W+TLNEPWTF+V                + EHV     +HRC  
Sbjct: 165 EYAELCFWEFGDRVKHWMTLNEPWTFSV-HGYATGLYAPGRGRTSPEHVNHPTVQHRCST 223

Query: 493 PHADDACEDGNPGTEPYIVTHNLILAHGEAVKLYRENY-KCQGGKIGITLVSQWWEP 660
                 C  GNPGTEPY VTH+L+LAH  AV+LY+  + + Q G+IGI+  +QW EP
Sbjct: 224 VAPQCICSTGNPGTEPYWVTHHLLLAHAAAVELYKNKFQRGQEGQIGISHATQWMEP 280


>pdb|3U57|A Chain A, Structures Of Alkaloid Biosynthetic Glucosidases Decode
           Substrate Specificity gi|358439929|pdb|3U57|B Chain B,
           Structures Of Alkaloid Biosynthetic Glucosidases Decode
           Substrate Specificity gi|358439930|pdb|3U5U|A Chain A,
           Structures Of Alkaloid Biosynthetic Glucosidases Decode
           Substrate Specificity gi|358439931|pdb|3U5U|B Chain B,
           Structures Of Alkaloid Biosynthetic Glucosidases Decode
           Substrate Specificity gi|358439932|pdb|3U5Y|A Chain A,
           Structures Of Alkaloid Biosynthetic Glucosidases Decode
           Substrate Specificity gi|358439933|pdb|3U5Y|B Chain B,
           Structures Of Alkaloid Biosynthetic Glucosidases Decode
           Substrate Specificity gi|451928645|pdb|4EK7|A Chain A,
           High Speed X-ray Analysis Of Plant Enzymes At Room
           Temperature gi|451928646|pdb|4EK7|B Chain B, High Speed
           X-ray Analysis Of Plant Enzymes At Room Temperature
          Length = 513

 Score =  220 bits (560), Expect = 2e-55
 Identities = 106/177 (59%), Positives = 127/177 (71%), Gaps = 1/177 (0%)
 Frame = +1

Query: 133 GGRLSAGVNKEGVQYYNNLINELLANGIEPYVTLFHWDVPQALESEYGGFLDRRIVDDFR 312
           GGRLS GVNKEG+ YYNNLI+ LLANGI+P+VTLFHWDVPQALE EYGGFL  RIVDDF 
Sbjct: 105 GGRLSGGVNKEGINYYNNLIDGLLANGIKPFVTLFHWDVPQALEDEYGGFLSPRIVDDFC 164

Query: 313 EFADLCFWEFGDRVKNWITLNEPWTFAVLXXXXXXXXXXXXVVNTTEHVKDEVPKHRCHC 492
           E+A+LCFWEFGDRVK+W+TLN+PWTF+V                + EHV     +HRC  
Sbjct: 165 EYAELCFWEFGDRVKHWMTLNQPWTFSV-HGYATGLYAPGRGRTSPEHVNHPTVQHRCST 223

Query: 493 PHADDACEDGNPGTEPYIVTHNLILAHGEAVKLYRENY-KCQGGKIGITLVSQWWEP 660
                 C  GNPGTEPY VTH+L+LAH  AV+LY+  + + Q G+IGI+  +QW EP
Sbjct: 224 VAPQCICSTGNPGTEPYWVTHHLLLAHAAAVELYKNKFQRGQEGQIGISHATQWMEP 280


>emb|CAC08209.2| beta-glucosidase [Cicer arietinum]
          Length = 439

 Score =  201 bits (512), Expect = 8e-50
 Identities = 99/176 (56%), Positives = 119/176 (67%), Gaps = 1/176 (0%)
 Frame = +1

Query: 136 GRLSAGVNKEGVQYYNNLINELLANGIEPYVTLFHWDVPQALESEYGGFLDRRIVDDFRE 315
           G+LS G N EG+QYYNNLINE LA+GI+PY+TLFHWD+PQALE EYGGFL+  I++DFR+
Sbjct: 54  GKLSGGKNPEGIQYYNNLINESLAHGIKPYITLFHWDLPQALEDEYGGFLNSSIINDFRD 113

Query: 316 FADLCFWEFGDRVKNWITLNEPWTFAVLXXXXXXXXXXXXVVNTTEHVKDEVPKHRCHCP 495
           +ADLCF EFGDRVK+W+T NEPW F                 +   +    +   RC   
Sbjct: 114 YADLCFHEFGDRVKDWVTFNEPWMF-----------------SNGGYAVGSLAPGRC--- 153

Query: 496 HADDACEDGNPGTEPYIVTHNLILAHGEAVKLYRENYKC-QGGKIGITLVSQWWEP 660
            +D  C  GN GTEPY VTHN ILAH  AV++YR  YK  Q GKIGITLVS W+ P
Sbjct: 154 -SDPTCLGGNSGTEPYTVTHNQILAHAHAVRVYRTKYKAKQNGKIGITLVSNWFLP 208