BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cephaelis21_contig00023418
         (3039 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAN15318.1| isoamylase isoform 2 [Solanum tuberosum]               976   0.0  
ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti...   951   0.0  
ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|2...   918   0.0  
ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like ...   886   0.0  
ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2...   885   0.0  

>gb|AAN15318.1| isoamylase isoform 2 [Solanum tuberosum]
          Length = 878

 Score =  976 bits (2523), Expect = 0.0
 Identities = 505/859 (58%), Positives = 611/859 (71%), Gaps = 14/859 (1%)
 Frame = +2

Query: 242  VSLTMPWHFLGCGDMESAKLVSASHRRNTKGVMCCLKKVNSKDQKIHQVARCVGGNSQQA 421
            + L +    L  G  ES KLV +S     K ++C L+K+  +D     + R    N Q+A
Sbjct: 6    IQLAVHSRLLSYGSTESTKLVPSSSGNRGK-IVCSLRKLELEDMNFSGIGR---NNDQEA 61

Query: 422  ----FRLGSWGTSDTAVVETAKELSHYHFRTETNDRLKVLVEKTIYKYRVQVK-----FS 574
                 R  +   S  ++V +AK +  Y FRT+   ++KVLVE+T  KY+V V+      S
Sbjct: 62   PRRAHRRKALSASRISLVPSAKRVPTYLFRTDIGGQVKVLVERTNGKYKVLVEVLPLELS 121

Query: 575  GRENELFMSWGLFRSDSSC----DLQGSSLDENYSANETPFLQEPSGTLKVELEFDLSVA 742
               +EL M WGLFRSD+SC    DL     D   S  ETPF+Q PSG + VEL+F+ S+A
Sbjct: 122  YAHSELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPFVQGPSGKVTVELDFEASLA 181

Query: 743  PFYISFLLKPQLNSDMKDSCIGSHRKTSFVVPVGFSSGHPIPLGLSFSAEGYLNLALFSQ 922
            PFYISF +K QL SDM++S I SHR T+FVVPVG SSGHP PLG+SF  +G +N ALFS+
Sbjct: 182  PFYISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPAPLGISFQPDGSVNFALFSR 241

Query: 923  SAERVILCLYDSLKSEKPALEIDLDPYINKSGDVWHASIDGTLPFVRYGYRCKLGADDGQ 1102
            SA  V+LCLYD +  EKP+LEIDLDPYIN+SGD+WHA++D +LPF  YGYRCK       
Sbjct: 242  SARSVVLCLYDDISVEKPSLEIDLDPYINRSGDIWHAALDCSLPFKTYGYRCKATTSGKG 301

Query: 1103 EQVLLDPYAKVIGN-LPACTQSSSVVACLGQLCEEPPFDWGQEPRPCLQMEELIVYRLNV 1279
            E VLLDPYAKVI   +P    S      LG+LC EP +DW  +  P L ME+LI+YRLNV
Sbjct: 302  ELVLLDPYAKVIRRVIPRQGGSEIRPKYLGELCLEPGYDWSGDVPPSLPMEKLIIYRLNV 361

Query: 1280 MRFTKDNSSKLPNNIGGTFSGITEKLYHFKSLGMNAILLEPIFSFDEQKGPYFPWHFFSP 1459
             +FTKD SSKLP+++ GTFSGI+EK +HFK LG+NA+LLEPIF FDEQKGPYFPWHFFSP
Sbjct: 362  TQFTKDKSSKLPDDLAGTFSGISEKWHHFKDLGVNAMLLEPIFPFDEQKGPYFPWHFFSP 421

Query: 1460 ASQYGSPGDQVSVITSLKEMVKELHNNGIEIFLEVVFTRTADIASLRAIDNSSYYYAETG 1639
             + YG  GD +S I S+K+MVK+LH NGIE+FLEVVFT TA+ A L  +DN SY   + G
Sbjct: 422  GNMYGPSGDPLSAIKSMKDMVKKLHANGIEVFLEVVFTHTAEDAPLMNVDNFSYCI-KGG 480

Query: 1640 DNLGSKSALNCNYPVVTQMILDSLRHWVIEFHIDGFCFINASSLLRGFHGEVLSRPPLVE 1819
              L  ++ALNCNYP+V QMILD LRHWVIEFHIDGF F+NASSLLRGF+GE+LSRPPLVE
Sbjct: 481  QYLNIQNALNCNYPIVQQMILDCLRHWVIEFHIDGFVFVNASSLLRGFNGEILSRPPLVE 540

Query: 1820 AIALDPLLSKVKIIADTWDPYAMKPKEISFPHWKKWAEINNKFCFDVRNFLRGEGLLSKL 1999
            AIA DP+LSKVK+IAD W+P     KE  FPHW++WAEIN +FC D+R+FLRGEGLLS L
Sbjct: 541  AIAFDPILSKVKMIADNWNPLTNDSKENLFPHWRRWAEINMRFCDDIRDFLRGEGLLSNL 600

Query: 2000 ATRLCGSGDSFLDGRGPAFSFNFIARNFGLSLVDLVCFSSTKLSSELSWNCGEEGPTNKI 2179
            ATRLCGSGD F  GRGPAFSFN+IARN GL+LVDLV FSS +++SELSWNCG+EG T   
Sbjct: 601  ATRLCGSGDIFAGGRGPAFSFNYIARNSGLTLVDLVSFSSNEVASELSWNCGQEGATTNN 660

Query: 2180 SILETRVKQIRNFLFILFTSLGVPTLNMGDECGQSSGGSLAYADRIPFDWNALRTEFGIQ 2359
             +LE R+KQ+RNFLFILF SLGVP LNMGDECGQSSGG  AY  R    WN L+T FG Q
Sbjct: 661  IVLERRLKQVRNFLFILFISLGVPVLNMGDECGQSSGGPPAYDARKSLGWNTLKTGFGTQ 720

Query: 2360 TLQFISFXXXXXXXXXXXXXXXNFLKEDAIEWHGSNQSSPSWDDPRSKFLAMTLKSNAAV 2539
              QFISF                FLKE+ I+WHGS+QS P WD P SKFLAMTLK++A V
Sbjct: 721  IAQFISFLSNLRMRRSDLLQKRTFLKEENIQWHGSDQSPPKWDGPSSKFLAMTLKADAEV 780

Query: 2540 DKSGHGPNLFGDLYAAFNAAEQSESITLPPPPAHMVWFRLVDTALPFPGFFTADGAIVED 2719
             ++    ++ GDL+ AFN A  SE + LPPPP  MVW RLVDTALPFPGFF   G  VED
Sbjct: 781  SQT-LVSDIVGDLFVAFNGAGDSEIVILPPPPTDMVWHRLVDTALPFPGFFDEKGTPVED 839

Query: 2720 ELGEYEVQSHSCALFEARQ 2776
            EL  YE++SHSC LFEA++
Sbjct: 840  ELVAYEMKSHSCLLFEAQR 858


>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
          Length = 882

 Score =  951 bits (2458), Expect = 0.0
 Identities = 492/867 (56%), Positives = 617/867 (71%), Gaps = 33/867 (3%)
 Frame = +2

Query: 269  LGCGDMESAKLVSASHRRNTKGVMCCLKKVNSKDQKIHQVARCVGGNSQQAFRLGSW--- 439
            + CG  +S+KLV+A+H      V   L+K++ + ++I  V       ++  FR  +W   
Sbjct: 15   VNCGARDSSKLVAATHYICGNKVAHGLEKMDLERKEILGVV--AQNATRNCFRNINWKVS 72

Query: 440  GTSDTAVVETAKELS-----------HYHFRTETNDRLKVLVEKTIYKYRVQVKFSG--- 577
             TS  A+ ET    S           +Y F TE   ++KV+V K   KY V ++ S    
Sbjct: 73   ATSHIAIEETENRYSTTESEELESRLNYLFLTEIGGQVKVIVGKKNKKYIVSIEVSSLQL 132

Query: 578  --RENELFMSWGLFRSDSSC----DLQGSSLDENYSANETPFLQEPSGTLKVELEFDLSV 739
               +N+L +SWG+FRS+SSC    D Q    +   +  E PF++  SG+  ++L+F+ + 
Sbjct: 133  YNSDNKLILSWGVFRSNSSCFMPVDFQNLVPEVGSNTTEIPFMERSSGSFALKLDFEANH 192

Query: 740  APFYISFLLKPQLNSDMKDSCIGSHRKTSFVVPVGFSSGHPIPLGLSFSAEGYLNLALFS 919
            APFY+SFLLK  L++D+  S I SHRKT+F +PVGF  G+P PLGLSFS++G  N A FS
Sbjct: 193  APFYLSFLLKSTLDTDLSSSDIRSHRKTNFCIPVGFGRGYPAPLGLSFSSDGSPNFAFFS 252

Query: 920  QSAERVILCLYDSLKSEKPALEIDLDPYINKSGDVWHASIDGTLPFVRYGYRCKLG--AD 1093
            ++A  V+LCLYD   S+KPALEIDLDPY+N++GD+WHAS++    FV YGYRCK     D
Sbjct: 253  RNAGGVVLCLYDGT-SDKPALEIDLDPYVNRTGDIWHASMESVGSFVSYGYRCKEANLQD 311

Query: 1094 DGQ----EQVLLDPYAKVIGNLPACTQSSSVVACLGQLCEEPPFDWGQEPRPCLQMEELI 1261
             G+    E V LDPYAK+I N  +          LG+L +EP F+W  +  P + ME+L+
Sbjct: 312  SGETLHTEHVHLDPYAKLIRNSFSDDHGLKPQPRLGELQKEPAFNWNDDVHPYIPMEKLV 371

Query: 1262 VYRLNVMRFTKDNSSKLPNNIGGTFSGITEKLYHFKSLGMNAILLEPIFSFDEQKGPYFP 1441
            VYRLNVM FTKD SS++ +++ GTFSG+ EKL+HFK LG+NA+LLEPIFSFDEQKGPYFP
Sbjct: 372  VYRLNVMHFTKDESSQVASDLAGTFSGLMEKLHHFKDLGVNAVLLEPIFSFDEQKGPYFP 431

Query: 1442 WHFFSPASQYGSPGDQVSVITSLKEMVKELHNNGIEIFLEVVFTRTADIASLRAIDNSSY 1621
            +HFFSP + YG     VS I S+KEMVK LH NGIE+ LEVVFT TA+  +L+ ID+S Y
Sbjct: 432  FHFFSPMNVYGPSSGPVSTINSVKEMVKRLHANGIEVLLEVVFTHTAESGALQGIDDSCY 491

Query: 1622 YYAETGDNLGSKSALNCNYPVVTQMILDSLRHWVIEFHIDGFCFINASSLLRGFHGEVLS 1801
            YY     +LG ++ALNCNY +V QMI+DSLR+WV EFH+DGFCFINASSLLRGFHGE LS
Sbjct: 492  YYVNGDADLGIRNALNCNYSIVQQMIVDSLRYWVTEFHVDGFCFINASSLLRGFHGEYLS 551

Query: 1802 RPPLVEAIALDPLLSKVKIIADTWDPYAMKPKEISFPHWKKWAEINNKFCFDVRNFLRGE 1981
            RPPLVE IA DPLLSK KIIAD WDP  M PKEI FPHWK+WAE+N +FC DVRNFLRGE
Sbjct: 552  RPPLVETIAFDPLLSKTKIIADCWDPRNMLPKEIRFPHWKRWAEVNTRFCNDVRNFLRGE 611

Query: 1982 GLLSKLATRLCGSGDSFLDGRGPAFSFNFIARNFGLSLVDLVCFSSTKLSSELSWNCGEE 2161
            G LS  ATRLCGSGD F+DGRGPAFSFNF  +NFGL LVDLV FSS++L+SELSWNCG+E
Sbjct: 612  G-LSDFATRLCGSGDIFMDGRGPAFSFNFTTKNFGLPLVDLVSFSSSELASELSWNCGDE 670

Query: 2162 GPTNKISILETRVKQIRNFLFILFTSLGVPTLNMGDECGQSSGGSLAYADRIPFDWNALR 2341
            GPTNK ++LE R+KQIRNFLFIL+ SLGVP LNMGDECGQSSGGS AY DR PF+WN+++
Sbjct: 671  GPTNKTTVLERRLKQIRNFLFILYVSLGVPILNMGDECGQSSGGSPAYGDRKPFNWNSVK 730

Query: 2342 TEFGIQTLQFISFXXXXXXXXXXXXXXXNFLKEDAIEWHGSNQSSPSWDDPRSKFLAMTL 2521
            T FGIQT+QFISF               +FLKE++I+WHGS+QS P WDDP SKFLAMTL
Sbjct: 731  TGFGIQTIQFISFLSSLRSRRSDLLQRRSFLKEESIDWHGSDQSPPRWDDPSSKFLAMTL 790

Query: 2522 KS-NAAVDKSGHGPNLFGDLYAAFNAAEQSESITLPPPPAHMVWFRLVDTALPFPGFFTA 2698
            K+ N          ++ GDL+ AFN A++S  + LPPPP  MVW RLVDTALPFPGFFTA
Sbjct: 791  KAENMEGQLPSESSSIKGDLFIAFNTADRSVKVILPPPPTGMVWHRLVDTALPFPGFFTA 850

Query: 2699 DG-AIVEDELG--EYEVQSHSCALFEA 2770
            DG AI++ + G   Y+++SHSCALFEA
Sbjct: 851  DGEAILKKKSGLVTYKMESHSCALFEA 877


>ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|223527143|gb|EEF29318.1|
            isoamylase, putative [Ricinus communis]
          Length = 872

 Score =  918 bits (2372), Expect = 0.0
 Identities = 463/792 (58%), Positives = 566/792 (71%), Gaps = 17/792 (2%)
 Frame = +2

Query: 446  SDTAVVETAKELSHYHFRTETNDRLKVLVEKTIYKYRVQVKFSGRE-----NELFMSWGL 610
            S +A ++   ++S Y FRT+    +KVLV K   KY V ++ S  E       L + WG+
Sbjct: 78   STSAPLDELNKVSTYLFRTQFGGHVKVLVRKKNAKYAVYIEVSSLELGTTDYRLMLIWGI 137

Query: 611  FRSDSSC--DLQGSSLDENYSANETPFLQEPSGTLKVELEFDLSVAPFYISFLLKPQLNS 784
            +RSDSSC   L   +   N    +T  +Q   GT  +ELEF+    PFY+SFLLK +LN+
Sbjct: 138  YRSDSSCFMPLDSQNFAPNARKMDTALVQNSFGTFALELEFEPKQTPFYLSFLLKSKLNT 197

Query: 785  DMKDSCIGSHRKTSFVVPVGFSSGHPIPLGLSFSAEGYLNLALFSQSAERVILCLYDSLK 964
            D     I +H+  +F VP+GF+SG P PLGLSFS +G +N A FS++ E ++LCLYD   
Sbjct: 198  DASGLEIKNHKNANFCVPIGFNSGDPSPLGLSFSTDGSMNFAFFSRNVEGLVLCLYDDST 257

Query: 965  SEKPALEIDLDPYINKSGDVWHASIDGTLPFVRYGYRCKLGADDGQ------EQVLLDPY 1126
            ++KPALE+DLDPY+N++GDVWHAS++G   F  YGYRCK     G       E VLLDPY
Sbjct: 258  TDKPALELDLDPYVNRTGDVWHASLEGAWTFTSYGYRCKGAILQGNTSKVDMECVLLDPY 317

Query: 1127 AKVIGNLPACTQSSSVVACLGQLCEEPPFDWGQEPRPCLQMEELIVYRLNVMRFTKDNSS 1306
            A+VI +      S      LG+LCEEP F+WG + RP L ME+LIVYRLNV RFT+  S 
Sbjct: 318  ARVIASSMTDHGSRLSAKYLGRLCEEPAFEWGSDIRPNLAMEKLIVYRLNVKRFTEHKSG 377

Query: 1307 KLPNNIGGTFSGITEKLYHFKSLGMNAILLEPIFSFDEQKGPYFPWHFFSPASQYGSPGD 1486
            KL ++I GTF+G+ EK+ HF++LG+NA+LLEPIF FDEQKGPYFP+HFFSP++ YG  G 
Sbjct: 378  KLYSDIAGTFAGLIEKMDHFRNLGVNAVLLEPIFPFDEQKGPYFPYHFFSPSNIYGPSGG 437

Query: 1487 QVSVITSLKEMVKELHNNGIEIFLEVVFTRTADIASLRAIDNSSYYYAETGDNLGSKSAL 1666
             +S ITS+KEMVKELH N IE+ LEVVFT TA+  +L+ ID+ SYYY  T  ++ S++AL
Sbjct: 438  SISAITSMKEMVKELHANRIEVLLEVVFTHTAEGGALQGIDDFSYYY--TKSSMDSRNAL 495

Query: 1667 NCNYPVVTQMILDSLRHWVIEFHIDGFCFINASSLLRGFHGEVLSRPPLVEAIALDPLLS 1846
            NCNYP+V +MILDSL+HWV EFHIDGFCFINAS+LL GFHGE LSRPPLVEAIA DP+LS
Sbjct: 496  NCNYPIVQRMILDSLQHWVTEFHIDGFCFINASALLTGFHGEHLSRPPLVEAIAFDPILS 555

Query: 1847 KVKIIADTWDPYAMKPKEISFPHWKKWAEINNKFCFDVRNFLRGEGLLSKLATRLCGSGD 2026
            K KIIAD W P    PKE  FPHWK+WAEIN KFC DVRNFLRGE LL  LATRLCGSGD
Sbjct: 556  KTKIIADPWHPEHRIPKETCFPHWKRWAEINPKFCIDVRNFLRGESLLGDLATRLCGSGD 615

Query: 2027 SFLDGRGPAFSFNFIARNFGLSLVDLVCFSSTKLSSELSWNCGEEGPTNKISILETRVKQ 2206
             F +GRGPAFSFN+IARN GL LVDLV FS  +L SELSWNCGEEGPTNK ++LE R+KQ
Sbjct: 616  IFSNGRGPAFSFNYIARNSGLPLVDLVSFSGGELGSELSWNCGEEGPTNKTAVLERRLKQ 675

Query: 2207 IRNFLFILFTSLGVPTLNMGDECGQSSGGSLAYADRIPFDWNALRTEFGIQTLQFISFXX 2386
            IRN+LFIL+ SLGVP LNMGDECGQSS GS++Y DR PFDWNAL T FG Q  QFISF  
Sbjct: 676  IRNYLFILYVSLGVPVLNMGDECGQSSRGSISYGDRKPFDWNALSTSFGNQMTQFISFLS 735

Query: 2387 XXXXXXXXXXXXXNFLKEDAIEWHGSNQSSPSWDDPRSKFLAMTLKSNAAVDKSGHGP-N 2563
                         NFLKE+ I+WHG++QS P W+DP  KFLAMTLK + A  +    P N
Sbjct: 736  SLRMRRSDLLQKRNFLKEENIDWHGNDQSPPRWEDPTCKFLAMTLKIDKAESQLSSEPSN 795

Query: 2564 LFGDLYAAFNAAEQSESITLPPPPAHMVWFRLVDTALPFPGFFTADGAIVEDELG---EY 2734
            + GDL+ AFNAA  +ES+ LPP P  M+W RLVDTALPFPGFF+ DG  V +++     Y
Sbjct: 796  IKGDLFMAFNAAGHAESVILPPVPEGMIWRRLVDTALPFPGFFSEDGEPVVEQIAGLIAY 855

Query: 2735 EVQSHSCALFEA 2770
            ++ SHSC LFEA
Sbjct: 856  KMNSHSCTLFEA 867


>ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like [Cucumis sativus]
          Length = 885

 Score =  886 bits (2289), Expect = 0.0
 Identities = 453/853 (53%), Positives = 586/853 (68%), Gaps = 21/853 (2%)
 Frame = +2

Query: 278  GDMESAKLVSASHRRNTKGVMCCLKKVNSKDQKIHQVARCVGGNSQQAFRLGSWGTSDTA 457
            G M+ A++++  H  N  G +   K  +    K +  +    G S Q  RLG  G S   
Sbjct: 43   GKMDEARMLA--HGENKVGAV---KSSHRNLSKTYAKSGISVGKSGQ--RLGIGGKS--- 92

Query: 458  VVETAKELSHYHFRTETNDRLKVLVEKTIYKYRVQVKFSGRE-----NELFMSWGLFRSD 622
              +  + ++ Y FRTE  D + V V K    + V ++    +       L +SWG++RSD
Sbjct: 93   --KEQRRVATYLFRTEFGDLVNVFVGKKGSTFTVNIEVPSMQLVSIDEALLLSWGVYRSD 150

Query: 623  SSC---DLQGSSLDENYSANETPFLQEPSGTLKVELEFDLSVAPFYISFLLKPQLNSDMK 793
            S+    + + S  DE   A ETPF++   G   VELEFD    PFY+SF+LK  +  D  
Sbjct: 151  SALVTPNFESSPPDETTGATETPFVKTSEGKFSVELEFDAKHTPFYLSFVLKYPMGVDSG 210

Query: 794  DSCIGSHRKTSFVVPVGFSSGHPIPLGLSFSAEGYLNLALFSQSAERVILCLYDSLKSEK 973
             S I SH+KTSF VPVGF  G+P PLGLS S +G +N ++FS SAE ++LCLY+   SEK
Sbjct: 211  SSEIRSHKKTSFSVPVGFGRGYPSPLGLSISGDGSVNFSIFSSSAESLVLCLYNDSTSEK 270

Query: 974  PALEIDLDPYINKSGDVWHASIDGTLPFVRYGYRCKLGAD----DGQE--QVLLDPYAKV 1135
            P LE+DLDPYIN+SG++WHAS +G   FV YGY+CK        DG E  ++++DPYAK+
Sbjct: 271  PLLELDLDPYINRSGNIWHASFEGASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKI 330

Query: 1136 IGNLPACTQSSSV-----VACLGQLCEEPPFDWGQEPRPCLQMEELIVYRLNVMRFTKDN 1300
            +   P+  +SS          LGQ+ + P FDW  E  P L ME+L VYRLNV RFT D 
Sbjct: 331  LA--PSIPKSSGQGLGLPSKFLGQISKVPTFDWDGEVHPNLPMEKLFVYRLNVERFTMDK 388

Query: 1301 SSKLPNNIGGTFSGITEKLYHFKSLGMNAILLEPIFSFDEQKGPYFPWHFFSPASQYGSP 1480
            SS+LP +I GTFSG+T+KL HFK+LG+NA+LLEPIF FDE++GPYFP+HFFSP + YG  
Sbjct: 389  SSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPS 448

Query: 1481 GDQVSVITSLKEMVKELHNNGIEIFLEVVFTRTADIASLRAIDNSSYYYAETGDNLGSKS 1660
            G  +S I S+KEMVKELH NG+E+ LEVV+T T+   +L+ ID+SSYY+     NL  KS
Sbjct: 449  GASISAINSMKEMVKELHANGVEVILEVVYTHTSGNGALQGIDDSSYYFTNRVANLEEKS 508

Query: 1661 ALNCNYPVVTQMILDSLRHWVIEFHIDGFCFINASSLLRGFHGEVLSRPPLVEAIALDPL 1840
            ALNCNYP+V Q++LDSLR+WV EFH+DGFCF+NAS LLRG HGE+LSRPP VEAIA DPL
Sbjct: 509  ALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLRGHHGELLSRPPFVEAIAFDPL 568

Query: 1841 LSKVKIIADTWDPYAMKPKEISFPHWKKWAEINNKFCFDVRNFLRGEGLLSKLATRLCGS 2020
            LSK K++AD WDP  ++ KE  FPHWK+WAE+N+KFC D+R+F RGEGL+S LATRLCGS
Sbjct: 569  LSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFCSDIRDFFRGEGLISSLATRLCGS 628

Query: 2021 GDSFLDGRGPAFSFNFIARNFGLSLVDLVCFSSTKLSSELSWNCGEEGPTNKISILETRV 2200
            GD F DGRGPAFSFNFIARN GL LVDLV FS++ L+SELSWNCGEEGPT+ + +LE R+
Sbjct: 629  GDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSNSNLASELSWNCGEEGPTSNLKVLEKRL 688

Query: 2201 KQIRNFLFILFTSLGVPTLNMGDECGQSSGGSLAYADRIPFDWNALRTEFGIQTLQFISF 2380
            KQIRNF+F+LF SLGVP LNMGDECGQSSGGS+A+ D+  F+W+ L+T+FG QT QFI+F
Sbjct: 689  KQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFNDKRSFNWDLLKTDFGTQTTQFIAF 748

Query: 2381 XXXXXXXXXXXXXXXNFLKEDAIEWHGSNQSSPSWDDPRSKFLAMTLKSNAAVDKS-GHG 2557
                           NFLK + I+W  +NQS P W+D   KFLA+ L+++   ++S    
Sbjct: 749  LSSFRSRRFDLFQNRNFLKGENIDWFDNNQSPPQWEDASCKFLAVMLRADKEENESITEN 808

Query: 2558 PNLFGDLYAAFNAAEQSESITLPPPPAHMVWFRLVDTALPFPGFFTADGAIVE-DELGEY 2734
            P    +++  FNA++QSES+ LP P     WFR+VDTALPFPGFF++DG +V       Y
Sbjct: 809  PKTRSNIFMVFNASDQSESVALPEPLEGTSWFRVVDTALPFPGFFSSDGELVPMTGSVTY 868

Query: 2735 EVQSHSCALFEAR 2773
            E+Q+HSCALFEA+
Sbjct: 869  EIQAHSCALFEAK 881


>ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
            [Cucumis sativus]
          Length = 885

 Score =  885 bits (2288), Expect = 0.0
 Identities = 453/853 (53%), Positives = 586/853 (68%), Gaps = 21/853 (2%)
 Frame = +2

Query: 278  GDMESAKLVSASHRRNTKGVMCCLKKVNSKDQKIHQVARCVGGNSQQAFRLGSWGTSDTA 457
            G M+ A++++  H  N  G +   K  +    K +  +    G S Q  RLG  G S   
Sbjct: 43   GKMDEARMLA--HGENKVGAV---KSSHRNLSKTYAKSGISVGKSGQ--RLGIGGKS--- 92

Query: 458  VVETAKELSHYHFRTETNDRLKVLVEKTIYKYRVQVKFSGRE-----NELFMSWGLFRSD 622
              +  + ++ Y FRTE  D + V V K    + V ++    +       L +SWG++RSD
Sbjct: 93   --KEQRRVATYLFRTEFGDLVNVFVGKKGSTFTVNIEVPSMQLVSIDEALLLSWGVYRSD 150

Query: 623  SSC---DLQGSSLDENYSANETPFLQEPSGTLKVELEFDLSVAPFYISFLLKPQLNSDMK 793
            S+    + + S  DE   A ETPF++   G   VELEFD    PFY+SF+LK  +  D  
Sbjct: 151  SALVTPNFESSPPDETTGATETPFVKTSEGKFSVELEFDAKHTPFYLSFVLKYPMGVDSG 210

Query: 794  DSCIGSHRKTSFVVPVGFSSGHPIPLGLSFSAEGYLNLALFSQSAERVILCLYDSLKSEK 973
             S I SH+KTSF VPVGF  G+P PLGLS S +G +N ++FS SAE ++LCLY+   SEK
Sbjct: 211  SSEIRSHKKTSFSVPVGFGRGYPSPLGLSISGDGSVNFSIFSSSAESLVLCLYNDSTSEK 270

Query: 974  PALEIDLDPYINKSGDVWHASIDGTLPFVRYGYRCKLGAD----DGQE--QVLLDPYAKV 1135
            P LE+DLDPYIN+SG++WHAS +G   FV YGY+CK        DG E  ++++DPYAK+
Sbjct: 271  PLLELDLDPYINRSGNIWHASFEGASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKI 330

Query: 1136 IGNLPACTQSSSV-----VACLGQLCEEPPFDWGQEPRPCLQMEELIVYRLNVMRFTKDN 1300
            +   P+  +SS          LGQ+ + P FDW  E  P L ME+L VYRLNV RFT D 
Sbjct: 331  LA--PSIPKSSGQGLGLPSKFLGQISKVPTFDWDGEVHPNLPMEKLXVYRLNVERFTMDK 388

Query: 1301 SSKLPNNIGGTFSGITEKLYHFKSLGMNAILLEPIFSFDEQKGPYFPWHFFSPASQYGSP 1480
            SS+LP +I GTFSG+T+KL HFK+LG+NA+LLEPIF FDE++GPYFP+HFFSP + YG  
Sbjct: 389  SSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPS 448

Query: 1481 GDQVSVITSLKEMVKELHNNGIEIFLEVVFTRTADIASLRAIDNSSYYYAETGDNLGSKS 1660
            G  +S I S+KEMVKELH NG+E+ LEVV+T T+   +L+ ID+SSYY+     NL  KS
Sbjct: 449  GASISAINSMKEMVKELHANGVEVILEVVYTHTSGNGALQGIDDSSYYFTNRVANLEEKS 508

Query: 1661 ALNCNYPVVTQMILDSLRHWVIEFHIDGFCFINASSLLRGFHGEVLSRPPLVEAIALDPL 1840
            ALNCNYP+V Q++LDSLR+WV EFH+DGFCF+NAS LLRG HGE+LSRPP VEAIA DPL
Sbjct: 509  ALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLRGHHGELLSRPPFVEAIAFDPL 568

Query: 1841 LSKVKIIADTWDPYAMKPKEISFPHWKKWAEINNKFCFDVRNFLRGEGLLSKLATRLCGS 2020
            LSK K++AD WDP  ++ KE  FPHWK+WAE+N+KFC D+R+F RGEGL+S LATRLCGS
Sbjct: 569  LSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFCSDIRDFFRGEGLISSLATRLCGS 628

Query: 2021 GDSFLDGRGPAFSFNFIARNFGLSLVDLVCFSSTKLSSELSWNCGEEGPTNKISILETRV 2200
            GD F DGRGPAFSFNFIARN GL LVDLV FS++ L+SELSWNCGEEGPT+ + +LE R+
Sbjct: 629  GDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSNSNLASELSWNCGEEGPTSNLKVLEKRL 688

Query: 2201 KQIRNFLFILFTSLGVPTLNMGDECGQSSGGSLAYADRIPFDWNALRTEFGIQTLQFISF 2380
            KQIRNF+F+LF SLGVP LNMGDECGQSSGGS+A+ D+  F+W+ L+T+FG QT QFI+F
Sbjct: 689  KQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFNDKRSFNWDLLKTDFGTQTTQFIAF 748

Query: 2381 XXXXXXXXXXXXXXXNFLKEDAIEWHGSNQSSPSWDDPRSKFLAMTLKSNAAVDKS-GHG 2557
                           NFLK + I+W  +NQS P W+D   KFLA+ L+++   ++S    
Sbjct: 749  LSSFRSRRFDLFQNRNFLKGENIDWFDNNQSPPQWEDASCKFLAVMLRADKEENESITEN 808

Query: 2558 PNLFGDLYAAFNAAEQSESITLPPPPAHMVWFRLVDTALPFPGFFTADGAIVE-DELGEY 2734
            P    +++  FNA++QSES+ LP P     WFR+VDTALPFPGFF++DG +V       Y
Sbjct: 809  PKTRSNIFMVFNASDQSESVALPEPLEGTSWFRVVDTALPFPGFFSSDGELVPMTGSVTY 868

Query: 2735 EVQSHSCALFEAR 2773
            E+Q+HSCALFEA+
Sbjct: 869  EIQAHSCALFEAK 881