BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cephaelis21_contig00017636
         (1349 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACM92061.1| tabersonine 16-hydroxylase CYP71D12 [Catharanthus...   444   e-122
ref|XP_003634756.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P...   444   e-122
sp|P98183.1|C71DC_CATRO RecName: Full=Tabersonine 16-hydroxylase...   441   e-121
ref|XP_002522762.1| cytochrome P450, putative [Ricinus communis]...   435   e-119
ref|XP_002522721.1| cytochrome P450, putative [Ricinus communis]...   432   e-118

>gb|ACM92061.1| tabersonine 16-hydroxylase CYP71D12 [Catharanthus roseus]
            gi|325989357|gb|ADZ48683.1| tabersonine 16-hydroxylase
            [synthetic construct]
          Length = 506

 Score =  444 bits (1143), Expect = e-122
 Identities = 237/423 (56%), Positives = 294/423 (69%), Gaps = 9/423 (2%)
 Frame = +3

Query: 105  MEFITAL-CAFSAFLFMLIKFLKKSTKNSALXXXXXXXXXXXXXXX----HNLSGSLIHH 269
            MEF   L  AF  F F+L K  KK  +NS                     H LSG   HH
Sbjct: 1    MEFYYFLYLAFLLFCFILSKTTKKFGQNSQYSNHDELPPGPPQIPILGNAHQLSGGHTHH 60

Query: 270  TLRDLAKTYGPLMHLRLGEVTTLVVSSPELAEEFMKTHDTIFANRPQLLCP-RIFSYGCT 446
             LRDLAK YGPLMHL++GEV+T+V SSP++AEE  +THD +FA+RP  L   +I SY  +
Sbjct: 61   ILRDLAKKYGPLMHLKIGEVSTIVASSPQIAEEIFRTHDILFADRPSNLESFKIVSYDFS 120

Query: 447  DIAFSAYGEYWRQLRKMCVMELLSSKRVQSFRPIREEEVLHFIRGISSNQGSVINLSTKL 626
            D+  S YG YWRQLRK+ +MELLS K VQSFR IREEEVL+FI+ I S +G+ INLS ++
Sbjct: 121  DMVVSPYGNYWRQLRKISMMELLSQKSVQSFRSIREEEVLNFIKSIGSKEGTRINLSKEI 180

Query: 627  CSFTFGLTARVAFGKKSKYQEEFISHVVEATKMTSGFAIADVYPSVKFIQSISEMSPKFR 806
                +G+T R AFG+K+K  EEFI  + + TK  +   IAD++PS+KF+Q IS    K  
Sbjct: 181  SLLIYGITTRAAFGEKNKNTEEFIRLLDQLTKAVAEPNIADMFPSLKFLQLISTSKYKIE 240

Query: 807  KMQKLLDKTLENILDEHKEKSQEPLQ---GEAKEDLVDVLLNVQNSSDFGTPLTTDNIKA 977
            K+ K  D  +E IL  HKEK  +PL    GE KEDLVDVLLN+Q  +DF  PL   NIKA
Sbjct: 241  KIHKQFDVIVETILKGHKEKINKPLSQENGEKKEDLVDVLLNIQRRNDFEAPLGDKNIKA 300

Query: 978  VILDIFSAGSETSATTLEWAMSEMVKNPGVMKKAQEEVRKAFDGSGNVNESGIHELKYLQ 1157
            +I +IFSAG+ETS+TT++WAM EM+KNP VMKKAQEEVRK F+  GNV+E+ +H+LKYLQ
Sbjct: 301  IIFNIFSAGTETSSTTVDWAMCEMIKNPTVMKKAQEEVRKVFNEEGNVDETKLHQLKYLQ 360

Query: 1158 SVIKETFRLHPPVAMLLPRESSEQCKIKGFDIPAKSRVIINAWAISRDSQNWTDAEKFNP 1337
            +VIKET RLHPPV +LLPRE  EQCKIKG+ IP+KSRVI+NAWAI RD   W + EKFNP
Sbjct: 361  AVIKETLRLHPPVPLLLPRECREQCKIKGYTIPSKSRVIVNAWAIGRDPNYWIEPEKFNP 420

Query: 1338 ERF 1346
            +RF
Sbjct: 421  DRF 423


>ref|XP_003634756.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 71D10-like [Vitis
            vinifera]
          Length = 554

 Score =  444 bits (1141), Expect = e-122
 Identities = 233/452 (51%), Positives = 308/452 (68%), Gaps = 5/452 (1%)
 Frame = +3

Query: 6    LRSTVPNEVQNCQSLLTVLLQINPQ*H-FVP-SILMEFITALCAFSAFLFMLIKFLKKS- 176
            +RS+    V     L T+ +Q     H F+P  I +    A  AF   +F++ K  ++S 
Sbjct: 16   VRSSCETFVIRLLILTTIFIQYLCFLHSFIPMEIHLPSSYAFFAFLLSMFIVFKIXERSK 75

Query: 177  TKNSALXXXXXXXXXXXXXXXHNLSGSLIHHTLRDLAKTYGPLMHLRLGEVTTLVVSSPE 356
            +K S                 H L GSL HHTL+ LA  YGP MHL LGEV+ LVVSSPE
Sbjct: 76   SKISPAKLPPGPWKLPLIGNMHQLVGSLPHHTLKRLASKYGPFMHLELGEVSALVVSSPE 135

Query: 357  LAEEFMKTHDTIFANRPQLLCPRIFSYGCTDIAFSAYGEYWRQLRKMCVMELLSSKRVQS 536
            +A E MKTHDTIFA RP LL   I +Y  T I+FS YG+YWRQLRK+C +ELLS+KRV+S
Sbjct: 136  IAREVMKTHDTIFAQRPPLLSSTIINYNATSISFSPYGDYWRQLRKICTIELLSAKRVKS 195

Query: 537  FRPIREEEVLHFIRGISSNQGSVINLSTKLCSFTFGLTARVAFGKKSKYQEEFISHVVEA 716
            F+ IRE EV   I  IS N GS INLS K+ S T+G+T+R AFGKK + Q+ F+S ++EA
Sbjct: 196  FQSIREXEVSKLIWSISLNAGSPINLSEKIFSLTYGITSRSAFGKKFRGQDAFVSAILEA 255

Query: 717  TKMTSGFAIADVYPSVKFIQSISEMSPKFRKMQKLLDKTLENILDEHKEKSQEPLQG--E 890
             ++++GF +AD+YPS+K++  IS M PK  K+ + +D+ L NI+D+H+++      G  E
Sbjct: 256  VELSAGFCVADMYPSLKWLHYISGMKPKLEKVHQKIDRILNNIIDDHRKRKTTTKAGQPE 315

Query: 891  AKEDLVDVLLNVQNSSDFGTPLTTDNIKAVILDIFSAGSETSATTLEWAMSEMVKNPGVM 1070
             +EDLVDVLLN+Q   D G PLT  N+KAV+LDIFS G ETS+T + WAM+EM+K+P VM
Sbjct: 316  TQEDLVDVLLNLQEHGDLGIPLTDGNVKAVLLDIFSGGGETSSTAVVWAMAEMLKSPIVM 375

Query: 1071 KKAQEEVRKAFDGSGNVNESGIHELKYLQSVIKETFRLHPPVAMLLPRESSEQCKIKGFD 1250
            +KAQ EVR+ FDG  ++NE+GIHELKYL SV+KET RLHP V +LLPRE  E+C I G++
Sbjct: 376  EKAQAEVRRVFDGKRDINETGIHELKYLNSVVKETLRLHPSVPLLLPRECRERCVINGYE 435

Query: 1251 IPAKSRVIINAWAISRDSQNWTDAEKFNPERF 1346
            IP  ++VIINAWAI++D  +W +  KF PERF
Sbjct: 436  IPENTKVIINAWAIAQDPDHWFEPNKFFPERF 467


>sp|P98183.1|C71DC_CATRO RecName: Full=Tabersonine 16-hydroxylase; AltName: Full=Cytochrome
            P450 71D12 gi|5921278|emb|CAB56503.1| cytochrome P450
            [Catharanthus roseus]
          Length = 495

 Score =  441 bits (1134), Expect = e-121
 Identities = 231/410 (56%), Positives = 288/410 (70%), Gaps = 8/410 (1%)
 Frame = +3

Query: 141  FLFMLIKFLKKSTKNSALXXXXXXXXXXXXXXX----HNLSGSLIHHTLRDLAKTYGPLM 308
            F F+L K  KK  +NS                     H LSG   HH LRDLAK YGPLM
Sbjct: 3    FCFILSKTTKKFGQNSQYSNHDELPPGPPQIPILGNAHQLSGGHTHHILRDLAKKYGPLM 62

Query: 309  HLRLGEVTTLVVSSPELAEEFMKTHDTIFANRPQLLCP-RIFSYGCTDIAFSAYGEYWRQ 485
            HL++GEV+T+V SSP++AEE  +THD +FA+RP  L   +I SY  +D+  S YG YWRQ
Sbjct: 63   HLKIGEVSTIVASSPQIAEEIFRTHDILFADRPSNLESFKIVSYDFSDMVVSPYGNYWRQ 122

Query: 486  LRKMCVMELLSSKRVQSFRPIREEEVLHFIRGISSNQGSVINLSTKLCSFTFGLTARVAF 665
            LRK+ +MELLS K VQSFR IREEEVL+FI+ I S +G+ INLS ++    +G+T R AF
Sbjct: 123  LRKISMMELLSQKSVQSFRSIREEEVLNFIKSIGSKEGTRINLSKEISLLIYGITTRAAF 182

Query: 666  GKKSKYQEEFISHVVEATKMTSGFAIADVYPSVKFIQSISEMSPKFRKMQKLLDKTLENI 845
            G+K+K  EEFI  + + TK  +   IAD++PS+KF+Q IS    K  K+ K  D  +E I
Sbjct: 183  GEKNKNTEEFIRLLDQLTKAVAEPNIADMFPSLKFLQLISTSKYKIEKIHKQFDVIVETI 242

Query: 846  LDEHKEKSQEPLQ---GEAKEDLVDVLLNVQNSSDFGTPLTTDNIKAVILDIFSAGSETS 1016
            L  HKEK  +PL    GE KEDLVDVLLN+Q  +DF  PL   NIKA+I +IFSAG+ETS
Sbjct: 243  LKGHKEKINKPLSQENGEKKEDLVDVLLNIQRRNDFEAPLGDKNIKAIIFNIFSAGTETS 302

Query: 1017 ATTLEWAMSEMVKNPGVMKKAQEEVRKAFDGSGNVNESGIHELKYLQSVIKETFRLHPPV 1196
            +TT++WAM EM+KNP VMKKAQEEVRK F+  GNV+E+ +H+LKYLQ+VIKET RLHPPV
Sbjct: 303  STTVDWAMCEMIKNPTVMKKAQEEVRKVFNEEGNVDETKLHQLKYLQAVIKETLRLHPPV 362

Query: 1197 AMLLPRESSEQCKIKGFDIPAKSRVIINAWAISRDSQNWTDAEKFNPERF 1346
             +LLPRE  EQCKIKG+ IP+KSRVI+NAWAI RD   W + EKFNP+RF
Sbjct: 363  PLLLPRECREQCKIKGYTIPSKSRVIVNAWAIGRDPNYWIEPEKFNPDRF 412


>ref|XP_002522762.1| cytochrome P450, putative [Ricinus communis]
            gi|223538000|gb|EEF39613.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 507

 Score =  435 bits (1118), Expect = e-119
 Identities = 221/416 (53%), Positives = 282/416 (67%), Gaps = 4/416 (0%)
 Frame = +3

Query: 111  FITALCAFSAFLFMLIKFLKKS-TKNSALXXXXXXXXXXXXXXXHNLSGSLIHHTLRDLA 287
            F   L  F  F+FM+ +  KKS T + A                H+L GS  HH L+DLA
Sbjct: 7    FFPILFTFLLFIFMVFRIWKKSKTNHPAPHLPPGPWKLPLIGSMHHLVGSQPHHRLKDLA 66

Query: 288  KTYGPLMHLRLGEVTTLVVSSPELAEEFMKTHDTIFANRPQLLCPRIFSYGCTDIAFSAY 467
            K YGPLMHL+LGE+T +V+SSPE+A+E MKTHD +FA RP LL   + SY  TDIAF+ Y
Sbjct: 67   KKYGPLMHLQLGELTNIVISSPEIAKEVMKTHDVVFAQRPHLLAASVTSYNYTDIAFAPY 126

Query: 468  GEYWRQLRKMCVMELLSSKRVQSFRPIREEEVLHFIRGISSNQGSVINLSTKLCSFTFGL 647
            G+YWRQ+RK+C +ELL++KRVQSFR IREEEV   +R +SS+ GS IN S    S T+ +
Sbjct: 127  GDYWRQMRKLCTLELLTAKRVQSFRSIREEEVSRLMRSLSSSAGSPINFSRMFNSLTYSI 186

Query: 648  TARVAFGKKSKYQEEFISHVVEATKMTSGFAIADVYPSVKFIQSISEMSPKFRKMQKLLD 827
             +R +FGK  K +E FI  V +      GF +ADVYPSVK +  IS M+P+ +++  ++D
Sbjct: 187  ISRASFGKIWKGEEIFIPIVKKLIVAAGGFTLADVYPSVKLLHWISGMAPRLKRIHHIVD 246

Query: 828  KTLENILDEHKEK---SQEPLQGEAKEDLVDVLLNVQNSSDFGTPLTTDNIKAVILDIFS 998
               +NI+D+H+ K   +   ++GE   DLVDVLLN Q   D   P+T DNIK +ILD F 
Sbjct: 247  NIFQNIIDDHRTKRAAANSSVEGEG--DLVDVLLNFQAQEDLAVPITNDNIKGIILDTFV 304

Query: 999  AGSETSATTLEWAMSEMVKNPGVMKKAQEEVRKAFDGSGNVNESGIHELKYLQSVIKETF 1178
            AGSETS+TT EWAMSE++KNP VM+KAQEEVR+ F   GNV+E  +HEL YL+ VI ET 
Sbjct: 305  AGSETSSTTAEWAMSELLKNPRVMEKAQEEVRRVFGEEGNVHEGRLHELNYLKWVINETL 364

Query: 1179 RLHPPVAMLLPRESSEQCKIKGFDIPAKSRVIINAWAISRDSQNWTDAEKFNPERF 1346
            RLHPP+ +LLPRE  E C I G+DIP KS+VI+N WAI RD   W DAEKF PERF
Sbjct: 365  RLHPPIPLLLPRECRESCVINGYDIPVKSKVIVNVWAIGRDPNCWMDAEKFYPERF 420


>ref|XP_002522721.1| cytochrome P450, putative [Ricinus communis]
            gi|223537959|gb|EEF39572.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 499

 Score =  432 bits (1110), Expect = e-118
 Identities = 225/406 (55%), Positives = 286/406 (70%), Gaps = 4/406 (0%)
 Frame = +3

Query: 141  FLFMLIKFLKKS-TKNSALXXXXXXXXXXXXXXX-HNLSGSLIHHTLRDLAKTYGPLMHL 314
            F++M++K  K+S +K+S L                H L GSL HH LRDLAK YG +MHL
Sbjct: 12   FIYMVLKLWKRSNSKHSTLKNLPPGPTKLPLIGNMHQLLGSLPHHRLRDLAKKYGSIMHL 71

Query: 315  RLGEVTTLVVSSPELAEEFMKTHDTIFANRPQLLCPRIFSYGCTDIAFSAYGEYWRQLRK 494
            +LGEV  +VVSSPE A+E MKTHD +FA RP LL   + +Y  TDIAFS Y +YWRQLRK
Sbjct: 72   QLGEVPHVVVSSPEAAKEVMKTHDIVFAQRPFLLAASVITYNFTDIAFSPYSDYWRQLRK 131

Query: 495  MCVMELLSSKRVQSFRPIREEEVLHFIRGISSNQGSVINLSTKLCSFTFGLTARVAFGKK 674
            +CV+ELLS+KRVQSFR IREEEV + I  ++S  G   N S KL S T+G+ AR +FGKK
Sbjct: 132  ICVLELLSAKRVQSFRSIREEEVSNLINTLTSFSGKPFNFSRKLFSSTYGIAARASFGKK 191

Query: 675  SKYQEEFISHVVEATKMTSGFAIADVYPSVKFIQSISEMSPKFRKMQKLLDKTLENILDE 854
             K QEEFI    + T++  GF++AD++PS+ F+  IS M  +  +++   D  LENI+D+
Sbjct: 192  YKDQEEFIKVAQQMTEVAGGFSLADLFPSIAFLHVISGMKSRLLRLRDDGDNILENIIDD 251

Query: 855  HKEKSQE-PLQGEAK-EDLVDVLLNVQNSSDFGTPLTTDNIKAVILDIFSAGSETSATTL 1028
            H+ ++      GE + +DLVDVLL  Q       PLTT+NIKAVILDIF AGSETS+TT+
Sbjct: 252  HRARNMTGKTSGEGEDDDLVDVLLRFQKGGQLEFPLTTNNIKAVILDIFIAGSETSSTTV 311

Query: 1029 EWAMSEMVKNPGVMKKAQEEVRKAFDGSGNVNESGIHELKYLQSVIKETFRLHPPVAMLL 1208
            EWAMSEM++NP VM+KAQEEVR+ F   GNV+E+ +HEL YL+ VIKET RLHPP  +LL
Sbjct: 312  EWAMSEMLRNPRVMEKAQEEVRRVFGKKGNVDEAELHELNYLKLVIKETLRLHPPAPLLL 371

Query: 1209 PRESSEQCKIKGFDIPAKSRVIINAWAISRDSQNWTDAEKFNPERF 1346
            PRES E C+I G+ IP  S+VI+NAWAI RD  NWT+AE F PERF
Sbjct: 372  PRESRESCEINGYYIPVNSKVIVNAWAIGRDPNNWTEAETFYPERF 417