BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cephaelis21_contig00012541
         (3312 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAA89316.1| gibberellin 3beta-hydroxylase [Nicotiana tabacum]     296   3e-77
gb|AAM12873.1|AF494090_1 gibberellin 3-oxidase 2 [Nicotiana sylv...   293   2e-76
gb|ACT99991.1| gibberellin 3-oxidase [Malus x domestica]              292   5e-76
gb|AFS18537.1| gibberellin 3-oxidase [Pyrus communis]                 290   1e-75
gb|AEW67998.1| gibberellin 3 oxidase 1 [Castanea mollissima]          290   2e-75

>dbj|BAA89316.1| gibberellin 3beta-hydroxylase [Nicotiana tabacum]
          Length = 371

 Score =  296 bits (757), Expect = 3e-77
 Identities = 143/192 (74%), Positives = 169/192 (88%), Gaps = 1/192 (0%)
 Frame = +3

Query: 2664 DVIERFEKEMKKLAVNLMWLILGTLDIKEEDMEWAG-PRGGLEGGNAAIQLNSYPACPEP 2840
            DVIE +E+EM+KLA  LMWL+LG+L I +E+++WA  P+G  +GG+AA+QLNSYPACP+P
Sbjct: 162  DVIEEYEREMEKLAGRLMWLMLGSLGITKEEVKWAVCPKGESKGGSAALQLNSYPACPDP 221

Query: 2841 DRAMGLAAHTDSTILTILHQSNTSGLQVLREGTSGWVTVPPLPGALVVNAGDLLHILSNG 3020
            DRAMGLAAHTDSTILTILHQ+NTSGLQV +EG SGWVTVPP PGALVVN GDLLHILSNG
Sbjct: 222  DRAMGLAAHTDSTILTILHQNNTSGLQVFKEG-SGWVTVPPFPGALVVNVGDLLHILSNG 280

Query: 3021 LFPTVFHRAIVNRTQHRLSIAYLYGPPSEVKICPLQKLIDQCHPPLYRPVTWSYYLDMKS 3200
            L+P+V HRA+VNRT+HRLS+AYLYGPPS VK+ PL KL+DQ HPPLYR VTWS YL  K+
Sbjct: 281  LYPSVLHRAVVNRTRHRLSVAYLYGPPSRVKVSPLAKLVDQRHPPLYRAVTWSEYLGTKA 340

Query: 3201 KIFDKALSSLRL 3236
            + FDKALSS+RL
Sbjct: 341  RHFDKALSSVRL 352



 Score =  166 bits (421), Expect = 3e-38
 Identities = 87/167 (52%), Positives = 113/167 (67%), Gaps = 6/167 (3%)
 Frame = +2

Query: 1781 SQVSDDLRPYPVYSHPKQLDLSSVDQLPDSHAWTSTSEDDYTRSGFCNQLESETVPVVDL 1960
            S++SD  R +      K LDL+S+ +LP+SHAWTS++  DY     CN    E++PV+DL
Sbjct: 3    SRISDSFRAHS----QKHLDLNSIKELPESHAWTSSN--DYPSENSCN---FESIPVIDL 53

Query: 1961 ---ADGNASELLDH---ACRTWGVFQVKNHGFPRGLLQEMESLTQSLFSLPMQQKLRAGR 2122
                + N   +L+H   AC+ WG FQ+ NH     LLQ++E   +SLFSLPMQQKL+A R
Sbjct: 54   DNYINNNNINVLEHIGQACKKWGAFQIINHNISERLLQDIELAGKSLFSLPMQQKLKAAR 113

Query: 2123 SSGQTSGYGMAPISSFFPKVMWSEGFTILGSPLEHARQLWPRDYNTF 2263
            S    +GYG+A ISSFF K+MWSEGFTI+GSPLEHARQ+WP DY  F
Sbjct: 114  SPEGVTGYGVARISSFFSKLMWSEGFTIVGSPLEHARQIWPHDYQKF 160


>gb|AAM12873.1|AF494090_1 gibberellin 3-oxidase 2 [Nicotiana sylvestris]
          Length = 370

 Score =  293 bits (750), Expect = 2e-76
 Identities = 143/192 (74%), Positives = 166/192 (86%), Gaps = 1/192 (0%)
 Frame = +3

Query: 2664 DVIERFEKEMKKLAVNLMWLILGTLDIKEEDMEWA-GPRGGLEGGNAAIQLNSYPACPEP 2840
            D IE +E+EM+KLA  LMWL+LG+L I +ED+ WA GP+G  +GG+AA+QLNSYPACP+P
Sbjct: 161  DAIEEYEREMEKLAGRLMWLMLGSLGITKEDVNWAVGPKGESKGGSAALQLNSYPACPDP 220

Query: 2841 DRAMGLAAHTDSTILTILHQSNTSGLQVLREGTSGWVTVPPLPGALVVNAGDLLHILSNG 3020
            DRAMGLAAHTDSTILTILHQ+NTSGLQV +EG SGWVTVPP  GALVVN GDLLHILSNG
Sbjct: 221  DRAMGLAAHTDSTILTILHQNNTSGLQVFKEG-SGWVTVPPFLGALVVNVGDLLHILSNG 279

Query: 3021 LFPTVFHRAIVNRTQHRLSIAYLYGPPSEVKICPLQKLIDQCHPPLYRPVTWSYYLDMKS 3200
             +P+V HRA+VNRT+HRLS+AYLYGPPS VKI PL KL+DQ HPPLYR +TWS YL  K+
Sbjct: 280  SYPSVLHRAVVNRTRHRLSVAYLYGPPSGVKISPLAKLVDQRHPPLYRAITWSEYLGTKA 339

Query: 3201 KIFDKALSSLRL 3236
            K FDKALSS+RL
Sbjct: 340  KHFDKALSSVRL 351



 Score =  167 bits (423), Expect = 2e-38
 Identities = 86/169 (50%), Positives = 116/169 (68%), Gaps = 5/169 (2%)
 Frame = +2

Query: 1784 QVSDDLRPYPVYSHPKQLDLSSVDQLPDSHAWTSTSEDDYTRSGFCNQLESETVPVVDLA 1963
            ++SD  R +      K LDL+S+ +LP+SHAWTS++  DY     CN    E++PV+DL 
Sbjct: 4    RISDSFRTHS----QKHLDLNSIKELPESHAWTSSN--DYPSENSCN---FESIPVIDLN 54

Query: 1964 DGNASEL--LDH---ACRTWGVFQVKNHGFPRGLLQEMESLTQSLFSLPMQQKLRAGRSS 2128
            + N + +  L+H   AC+ WG FQ+ NH     LLQ++E   ++LFSLPMQQKL+A RS 
Sbjct: 55   NNNHNNINVLEHIGQACKKWGAFQIINHNISERLLQDIELAGKNLFSLPMQQKLKAARSP 114

Query: 2129 GQTSGYGMAPISSFFPKVMWSEGFTILGSPLEHARQLWPRDYNTFWSSI 2275
               +GYG+A ISSFF K+MWSEGFTI+GSPLEHARQ+WP+DY  F  +I
Sbjct: 115  DGVTGYGVARISSFFSKLMWSEGFTIVGSPLEHARQIWPQDYKKFCDAI 163


>gb|ACT99991.1| gibberellin 3-oxidase [Malus x domestica]
          Length = 369

 Score =  292 bits (747), Expect = 5e-76
 Identities = 136/191 (71%), Positives = 166/191 (86%)
 Frame = +3

Query: 2664 DVIERFEKEMKKLAVNLMWLILGTLDIKEEDMEWAGPRGGLEGGNAAIQLNSYPACPEPD 2843
            ++IE +EKEMK LA  LMWL+LG+L I  ED++WAG +G  +G +AA+QLNSYPACPEPD
Sbjct: 163  NIIEEYEKEMKMLAGRLMWLMLGSLGISMEDIKWAGSKGEFKGASAALQLNSYPACPEPD 222

Query: 2844 RAMGLAAHTDSTILTILHQSNTSGLQVLREGTSGWVTVPPLPGALVVNAGDLLHILSNGL 3023
            RAMGLAAHTDST+LTIL+QSNTSGLQVLREGT GWVTVPP+PGALV+N GDL HILSNGL
Sbjct: 223  RAMGLAAHTDSTLLTILYQSNTSGLQVLREGT-GWVTVPPMPGALVINVGDLFHILSNGL 281

Query: 3024 FPTVFHRAIVNRTQHRLSIAYLYGPPSEVKICPLQKLIDQCHPPLYRPVTWSYYLDMKSK 3203
            +P+V HRA+VNRTQHRLS+AYL+GPP+ ++I PL KL+   HPPLYRP++W+ YL  K+K
Sbjct: 282  YPSVLHRAVVNRTQHRLSVAYLFGPPASIQISPLSKLVGPSHPPLYRPISWNEYLGTKAK 341

Query: 3204 IFDKALSSLRL 3236
             F+KALSS+RL
Sbjct: 342  HFNKALSSVRL 352



 Score =  174 bits (442), Expect = 1e-40
 Identities = 86/164 (52%), Positives = 117/164 (71%)
 Frame = +2

Query: 1784 QVSDDLRPYPVYSHPKQLDLSSVDQLPDSHAWTSTSEDDYTRSGFCNQLESETVPVVDLA 1963
            ++SD  + +PV    K  D +S+ +LPDS+AWT+   DD+  S   +    ++VPV++L+
Sbjct: 4    KLSDAFKSHPVNLQLKHPDFNSLHELPDSYAWTNN--DDHYPSFLTSFGADDSVPVINLS 61

Query: 1964 DGNASELLDHACRTWGVFQVKNHGFPRGLLQEMESLTQSLFSLPMQQKLRAGRSSGQTSG 2143
            D N  +L  HAC+TWGVFQV NHG  + LL ++ES  +SLFSLP+QQKL+A RS    SG
Sbjct: 62   DTNVLKLTGHACKTWGVFQVTNHGIQQKLLDDIESAGKSLFSLPVQQKLKAARSPDGISG 121

Query: 2144 YGMAPISSFFPKVMWSEGFTILGSPLEHARQLWPRDYNTFWSSI 2275
            YG A ISSFF K+MWSEGFTI+GSP++H RQLWP+DY+ F + I
Sbjct: 122  YGFARISSFFKKLMWSEGFTIVGSPVDHFRQLWPQDYDKFCNII 165


>gb|AFS18537.1| gibberellin 3-oxidase [Pyrus communis]
          Length = 371

 Score =  290 bits (743), Expect = 1e-75
 Identities = 136/191 (71%), Positives = 167/191 (87%)
 Frame = +3

Query: 2664 DVIERFEKEMKKLAVNLMWLILGTLDIKEEDMEWAGPRGGLEGGNAAIQLNSYPACPEPD 2843
            ++IE +EKEMK+LA  LMWL+LG+L I  ED++WAG +G  +G +AA+QLNSYPACPEPD
Sbjct: 163  NIIEEYEKEMKRLAGRLMWLMLGSLGISPEDVKWAGSKGEFKGASAALQLNSYPACPEPD 222

Query: 2844 RAMGLAAHTDSTILTILHQSNTSGLQVLREGTSGWVTVPPLPGALVVNAGDLLHILSNGL 3023
            RAMGLAAHTDST+LTIL+Q+NTSGLQVLR+GT GWVTVPP+ GALVVN GDL+HILSNG+
Sbjct: 223  RAMGLAAHTDSTLLTILYQNNTSGLQVLRDGT-GWVTVPPMAGALVVNVGDLIHILSNGV 281

Query: 3024 FPTVFHRAIVNRTQHRLSIAYLYGPPSEVKICPLQKLIDQCHPPLYRPVTWSYYLDMKSK 3203
            +P+V HRA+VNRTQHRLSIAYL+GPP+ V+I PL KL+   HPPLYRP+TW+ YL  K+K
Sbjct: 282  YPSVLHRAVVNRTQHRLSIAYLFGPPASVQISPLSKLVGPSHPPLYRPITWNEYLGTKAK 341

Query: 3204 IFDKALSSLRL 3236
             F+KALSS+RL
Sbjct: 342  HFNKALSSVRL 352



 Score =  176 bits (445), Expect = 5e-41
 Identities = 85/165 (51%), Positives = 118/165 (71%)
 Frame = +2

Query: 1781 SQVSDDLRPYPVYSHPKQLDLSSVDQLPDSHAWTSTSEDDYTRSGFCNQLESETVPVVDL 1960
            +++SD  + +P+    K  D +S+ +LPDS+AWT+   DD+  S   +    ++VPV+DL
Sbjct: 3    ARLSDAFKSHPLNLQLKHPDFNSLHELPDSYAWTNN--DDHYPSSLSSFGVDDSVPVIDL 60

Query: 1961 ADGNASELLDHACRTWGVFQVKNHGFPRGLLQEMESLTQSLFSLPMQQKLRAGRSSGQTS 2140
            ++ NA +L  HAC+TWGVFQV NHG P+ LL ++ES  +SLFSLP++QKL+A R     S
Sbjct: 61   SEPNALKLTGHACKTWGVFQVTNHGIPQKLLDDIESAGKSLFSLPVEQKLKAARPPDGIS 120

Query: 2141 GYGMAPISSFFPKVMWSEGFTILGSPLEHARQLWPRDYNTFWSSI 2275
            GYG A ISSFF K+MWSEGFTI+GSP++H RQLWP+DY  F + I
Sbjct: 121  GYGFARISSFFRKLMWSEGFTIVGSPVDHFRQLWPQDYEKFCNII 165


>gb|AEW67998.1| gibberellin 3 oxidase 1 [Castanea mollissima]
          Length = 368

 Score =  290 bits (741), Expect = 2e-75
 Identities = 139/191 (72%), Positives = 165/191 (86%)
 Frame = +3

Query: 2664 DVIERFEKEMKKLAVNLMWLILGTLDIKEEDMEWAGPRGGLEGGNAAIQLNSYPACPEPD 2843
            D+IE +EKEMKKLA  LMWLILG+L I  ED++WAGP+G  +G +AA+QLNSYPACP+PD
Sbjct: 160  DIIEEYEKEMKKLAGRLMWLILGSLGICNEDIKWAGPKGEFQGASAALQLNSYPACPDPD 219

Query: 2844 RAMGLAAHTDSTILTILHQSNTSGLQVLREGTSGWVTVPPLPGALVVNAGDLLHILSNGL 3023
            +AMGLAAHTDST+LTILHQ+NTSGLQVLREGT GWVTV P+ GALVVN GDLLHILSNGL
Sbjct: 220  QAMGLAAHTDSTLLTILHQNNTSGLQVLREGT-GWVTVDPVQGALVVNIGDLLHILSNGL 278

Query: 3024 FPTVFHRAIVNRTQHRLSIAYLYGPPSEVKICPLQKLIDQCHPPLYRPVTWSYYLDMKSK 3203
            +P+V HRA+VNRT++RLS+AYLYGPP  V+I PL KL+   HPPLYRPVTW+ YL  K+K
Sbjct: 279  YPSVLHRAMVNRTRYRLSMAYLYGPPDSVQISPLSKLLGPSHPPLYRPVTWNEYLGTKAK 338

Query: 3204 IFDKALSSLRL 3236
             F+KALS +RL
Sbjct: 339  HFNKALSCVRL 349



 Score =  192 bits (488), Expect = 5e-46
 Identities = 91/161 (56%), Positives = 118/161 (73%)
 Frame = +2

Query: 1781 SQVSDDLRPYPVYSHPKQLDLSSVDQLPDSHAWTSTSEDDYTRSGFCNQLESETVPVVDL 1960
            S++SD  + +PV  +PK  D +S+ +LPDS+ WT    D+Y     C    SE+VP++DL
Sbjct: 3    SRLSDAFKAHPVQLYPKHQDFNSLQELPDSYKWTQL--DEYPS---CESFSSESVPLIDL 57

Query: 1961 ADGNASELLDHACRTWGVFQVKNHGFPRGLLQEMESLTQSLFSLPMQQKLRAGRSSGQTS 2140
             D NA  L+ HAC+TWGVFQV NHG P  LL ++ES+++ LFSLP QQKL+A RS    S
Sbjct: 58   TDPNALTLVGHACKTWGVFQVTNHGIPTKLLDDIESVSRCLFSLPQQQKLKAARSPDGVS 117

Query: 2141 GYGMAPISSFFPKVMWSEGFTILGSPLEHARQLWPRDYNTF 2263
            GYG+A ISSFFPK+MWSEGFTI+GSPLEH RQLWP++Y+ F
Sbjct: 118  GYGLARISSFFPKLMWSEGFTIVGSPLEHFRQLWPQEYSKF 158