BLASTX nr result
ID: Cephaelis21_contig00008632
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cephaelis21_contig00008632
(2173 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAW82883.1| phytoalexin-deficient 4-1 protein [Solanum tubero... 682 0.0
gb|AAW82884.1| phytoalexin-deficient 4-2 protein [Solanum tubero... 669 0.0
ref|XP_003523213.1| PREDICTED: uncharacterized protein LOC100818... 652 0.0
emb|CBI21592.3| unnamed protein product [Vitis vinifera] 649 0.0
ref|XP_002275637.1| PREDICTED: uncharacterized protein LOC100257... 649 0.0
>gb|AAW82883.1| phytoalexin-deficient 4-1 protein [Solanum tuberosum]
Length = 578
Score = 682 bits (1761), Expect = 0.0
Identities = 350/616 (56%), Positives = 446/616 (72%), Gaps = 1/616 (0%)
Frame = +3
Query: 138 MDAEVSLFEPSEMLATLLASTPLLEESWKLCGQANSAAPQGYLIKQTGEVCYVAFSGVQI 317
M++E S FE SE LA L+ASTPLLEESWK+CG A+++ + + + G YV FSGV++
Sbjct: 1 MESEASSFESSETLAALVASTPLLEESWKVCGVADASVDSNFAVNRVGGTAYVGFSGVKL 60
Query: 318 LAGLDPDSQYLVPIERFSNGLFSALHREGEGGETAMVHAGLLQLFLSFYANPIFQNQMME 497
AG+D + LVP+ + LFS+L +G + AMVHAGLLQLF S Y++ +F++QM+E
Sbjct: 61 GAGVDQSCRNLVPLP---DELFSSLCLDG--ADPAMVHAGLLQLFQSVYSDNLFRDQMVE 115
Query: 498 ITSKSKSVVFTGHSVGGTIGSLSALWLLSCLHSISSSFAVICITFGSPMLGNKSLSKSIL 677
I + SKS+V TGHS+GG I SL LWLL L +I S VICITFGSPMLGN+S S+ IL
Sbjct: 116 IMNTSKSIVITGHSIGGAIASLLTLWLLCRLQTICS---VICITFGSPMLGNESFSRVIL 172
Query: 678 QERWGGNFLHVVGQHDIVPRLLFAPSAPLIPHLQYFFDFWHLYMKSQFSHQPPVQFYKDK 857
Q+RW G+F HVV QHDIVPRL F+PS QF ++
Sbjct: 173 QKRWAGHFCHVVSQHDIVPRLFFSPSCSF-------------------------QFISEE 207
Query: 858 G-AELFHVVMNSLEALTKGLQDSDQGNLFWPFGSYIFCTSKGSICVDNAVSVIKLLHLLL 1034
+LF VV++SL +++G S F P GSY+FCT+ G++CVDN + VIKLL+L L
Sbjct: 208 NKTQLFRVVLDSLGVVSRGECKSS----FCPSGSYLFCTNNGAVCVDNGMVVIKLLYLTL 263
Query: 1035 AKGSPCSSIEDHLNYENYVSRVSWQILSSNNFLDGNLPESSYEAGITLALQSSDIAPSEP 1214
GS SS+EDHL Y +++ +V WQ + + +F+ GN+PESSYEAGITLAL+S IA E
Sbjct: 264 LNGSQSSSLEDHLGYADFIRKVQWQFIENRSFMGGNIPESSYEAGITLALESLGIASHEL 323
Query: 1215 VFGPAKDSLKMAKQMGRTPSLNSANLAIGLSKITPLRAQIEWYKDTCDDSDDKMGYYDSF 1394
AK++LK AK++GRT +LNSANLAIGLSKI P RAQIEWYK +CD+S ++MGYYDSF
Sbjct: 324 NSEDAKEALKKAKKLGRTRNLNSANLAIGLSKINPFRAQIEWYKASCDNSVEQMGYYDSF 383
Query: 1395 KQRGASKKAFKVNMNRHKLARFWNDVISKLETNQLPHDFHKRSKWVNASQFYKLLVEPLD 1574
KQRGAS++ FKVNMNR KL +FW+ +I KLE N+LP+DFHKR+KWVNASQFYKL+VEPLD
Sbjct: 384 KQRGASRRGFKVNMNRIKLGQFWDSLIDKLEANELPYDFHKRAKWVNASQFYKLVVEPLD 443
Query: 1575 IAEYYKCGEHLRKGHYMEHGRAKRYKIFDKWWRDREVSANPPNSRSNFASLTQDSCFWAR 1754
IAEYY+ G HL KGHYM+HGR +RYKIFDKWW + E + P +RS FAS TQDSCFWA+
Sbjct: 444 IAEYYRTGMHLVKGHYMQHGRERRYKIFDKWW-NTENGTDHPTARSRFASSTQDSCFWAQ 502
Query: 1755 VEEARDWLNRVRSEGDVRIQSSLLEKINKFDQYAREMVDRKEVSKDVLAKNSSYNMFVEE 1934
VEEARD L +VR+EGD R+ +LE + KFDQYA+ +++ KEVS+DVLAKNSSY F+EE
Sbjct: 503 VEEARDSLIKVRAEGDARMFIKMLEDVTKFDQYAKRLIENKEVSQDVLAKNSSYTKFIEE 562
Query: 1935 WKRLKSQLQQLPSHFP 1982
WK +SQLQ L FP
Sbjct: 563 WKDFQSQLQLLQPQFP 578
>gb|AAW82884.1| phytoalexin-deficient 4-2 protein [Solanum tuberosum]
Length = 578
Score = 669 bits (1726), Expect = 0.0
Identities = 345/616 (56%), Positives = 438/616 (71%), Gaps = 1/616 (0%)
Frame = +3
Query: 138 MDAEVSLFEPSEMLATLLASTPLLEESWKLCGQANSAAPQGYLIKQTGEVCYVAFSGVQI 317
M++E S FE SE LA L+ASTPLLEESWK+CG A++ + + + G YV FSGV++
Sbjct: 1 MESEASSFESSETLAALVASTPLLEESWKVCGVADALVDSNFAVNRVGGTAYVGFSGVKL 60
Query: 318 LAGLDPDSQYLVPIERFSNGLFSALHREGEGGETAMVHAGLLQLFLSFYANPIFQNQMME 497
AG+D LVP+ + LFS+L +G + AMVHAGLLQLF S Y++ +F++Q +E
Sbjct: 61 GAGVDQSCLNLVPLP---DELFSSLCLDG--ADPAMVHAGLLQLFQSVYSDNLFRDQKVE 115
Query: 498 ITSKSKSVVFTGHSVGGTIGSLSALWLLSCLHSISSSFAVICITFGSPMLGNKSLSKSIL 677
I + SK +V TGHS+GG I SL LWLL L +I S VICITFGSPMLGN+S + IL
Sbjct: 116 IMNTSKLIVITGHSIGGAIASLLTLWLLCRLQTICS---VICITFGSPMLGNESFPRVIL 172
Query: 678 QERWGGNFLHVVGQHDIVPRLLFAPSAPLIPHLQYFFDFWHLYMKSQFSHQPPVQFYKDK 857
Q+RW G+F HVV QHDIVPRL F+PS QF ++
Sbjct: 173 QKRWAGHFCHVVSQHDIVPRLFFSPSCSF-------------------------QFISEE 207
Query: 858 G-AELFHVVMNSLEALTKGLQDSDQGNLFWPFGSYIFCTSKGSICVDNAVSVIKLLHLLL 1034
+LF VV++SL +++G S F P GSY+FCT+ G++CVDN + VIKLL+L L
Sbjct: 208 NKTQLFRVVLDSLGVVSRGECKSS----FCPSGSYLFCTNNGAVCVDNGMVVIKLLYLTL 263
Query: 1035 AKGSPCSSIEDHLNYENYVSRVSWQILSSNNFLDGNLPESSYEAGITLALQSSDIAPSEP 1214
GS SS+EDHL Y +++ +V WQ + + +F+ GN+PESSYEAGITLAL+S IA E
Sbjct: 264 LNGSQSSSLEDHLGYADFIRKVQWQFIENRSFMGGNIPESSYEAGITLALESLGIASHEL 323
Query: 1215 VFGPAKDSLKMAKQMGRTPSLNSANLAIGLSKITPLRAQIEWYKDTCDDSDDKMGYYDSF 1394
AK++LK AK++GRT +LNSANLAIGLSKI P RAQIEWYK +CD+S ++MGYYDSF
Sbjct: 324 NSEDAKEALKKAKKLGRTRNLNSANLAIGLSKINPFRAQIEWYKTSCDNSVEQMGYYDSF 383
Query: 1395 KQRGASKKAFKVNMNRHKLARFWNDVISKLETNQLPHDFHKRSKWVNASQFYKLLVEPLD 1574
KQRGAS++ FKVNMNR KL +FW+ +I KLE N+LP+DFHKR+KWVNASQFYKL+VEPLD
Sbjct: 384 KQRGASRRGFKVNMNRIKLGQFWDSLIDKLEANELPYDFHKRAKWVNASQFYKLVVEPLD 443
Query: 1575 IAEYYKCGEHLRKGHYMEHGRAKRYKIFDKWWRDREVSANPPNSRSNFASLTQDSCFWAR 1754
IAEYY+ G HL KGHYM+HGR +RYKIFDKWW + E + P +RS FAS TQDSCFWA+
Sbjct: 444 IAEYYRTGMHLVKGHYMQHGRERRYKIFDKWW-NTENGTDHPTARSRFASSTQDSCFWAQ 502
Query: 1755 VEEARDWLNRVRSEGDVRIQSSLLEKINKFDQYAREMVDRKEVSKDVLAKNSSYNMFVEE 1934
VEEARD L +VR+E D R +LE + KFDQYA+ +++ KEVS+DVLAKNSSY F+EE
Sbjct: 503 VEEARDGLIKVRAESDARKFIKMLEDVTKFDQYAKRLIENKEVSQDVLAKNSSYTKFIEE 562
Query: 1935 WKRLKSQLQQLPSHFP 1982
W +SQLQ L FP
Sbjct: 563 WNDFQSQLQLLQPQFP 578
>ref|XP_003523213.1| PREDICTED: uncharacterized protein LOC100818765 [Glycine max]
Length = 633
Score = 652 bits (1683), Expect = 0.0
Identities = 345/630 (54%), Positives = 435/630 (69%), Gaps = 12/630 (1%)
Frame = +3
Query: 147 EVSLFEPSEMLATLLASTPLLEESWKLCGQANSAAPQGYLIKQTGE--VCYVAFSGVQIL 320
E S FE S+MLATLLASTPLL ESW+LC + AP+ ++ +Q G V YVAF GV++
Sbjct: 5 EASPFETSDMLATLLASTPLLSESWRLCTTVAATAPRSFMTEQHGGGGVVYVAFPGVEMA 64
Query: 321 AGLDPDSQYLVPIERFSN-GLFSALHREGEGGETAMVHAGLLQLFLSFYANPIFQNQMME 497
AG D + LV +E + LFSA R EG E MVHAG+L L +F+ FQ QM+
Sbjct: 65 AGSDSICRNLVALESIGDVPLFSARRRNKEGDEPVMVHAGMLNLLSTFFEP--FQKQMLA 122
Query: 498 I--TSKSKSVVFTGHSVGGTIGSLSALWLLSCLHSISSSFAV--ICITFGSPMLGNKSLS 665
+ SK+KS+V TGHS+GG SL ALWLLS LH SS +V +CITFGSPMLGN S S
Sbjct: 123 LMGNSKTKSIVLTGHSIGGATASLCALWLLSYLHQTYSSISVSVLCITFGSPMLGNGSFS 182
Query: 666 KSILQERWGGNFLHVVGQHDIVPRLLFAPSAPLIPHLQYFFDFWHLYMKSQFSHQPPVQF 845
++IL+ERWGGNF HVV +HDI+PRLLFAP P + + FW L M + + V
Sbjct: 183 RAILRERWGGNFCHVVSKHDIMPRLLFAPITPYTAQINFLLQFWQLSMTAPGFGKLAVPI 242
Query: 846 YKDKGAELFHVVMNSLEALTKGLQDSDQGNLFWPFGSYIFCTSKGSICVDNAVSVIKLLH 1025
D+ ELF+ VM+ L+A T+ ++ LF PFGSY+F +S G++CVD A SVIK+LH
Sbjct: 243 -SDQQKELFNFVMSHLDAATQD-EEGSAPVLFHPFGSYLFVSSDGAVCVDCATSVIKMLH 300
Query: 1026 LLLAKGSPCSSIEDHLNYENYVSRVSWQILSSNNFLDGNLPESSYEAGITLALQSSDIAP 1205
L+ A SP SIEDHL Y +YV +S Q L+ NN + GN+P+SSYEAG+ L++QSS +
Sbjct: 301 LMFASVSPACSIEDHLKYGDYVKNLSLQFLNQNNSVQGNIPDSSYEAGLELSVQSSGLGN 360
Query: 1206 SEPVFGPAKDSLKMAKQMGRTPSLNSANLAIGLSKITPLRAQIEWYKDTCDDSDDKMGYY 1385
E PAK+ LKM ++MG +P+ N+ANL+I LSK P R +IEWYK C D+MGYY
Sbjct: 361 QESAIEPAKECLKMTRRMGPSPTKNAANLSITLSKFVPYRTEIEWYKAWCHQQVDQMGYY 420
Query: 1386 DSFK-QRGASKKAFKVNMNRHKLARFWNDVISKLETNQLPHDFHKRSKWVNASQFYKLLV 1562
D FK +R SK A KVNMNRHKLARFWN+VI E N+LPHD R+KWVNAS FYKLLV
Sbjct: 421 DLFKRRRSTSKMAMKVNMNRHKLARFWNNVIEMWERNELPHDVAVRAKWVNASHFYKLLV 480
Query: 1563 EPLDIAEYYKCGEHLRKGHYMEHGRAKRYKIFDKWWRDREVSANPPNS-RSNFASLTQDS 1739
EPLDIAEYY G H KGHY++HGR KRY+IFD+WW+D + N RS FASLTQDS
Sbjct: 481 EPLDIAEYYGKGMHTTKGHYIQHGREKRYEIFDRWWKDAMGNTEENNERRSKFASLTQDS 540
Query: 1740 CFWARVEEARDWLNRVRSEGDVRIQSSLLEKINKFDQYAREMVDRKEVSKDVLAKNSSYN 1919
CFWARVEEARDWLN VRSE D + L + I KF++YA E++D KEVS+DVLAKNSSY+
Sbjct: 541 CFWARVEEARDWLNSVRSESDTTKLAVLWDNIEKFEKYAMELIDNKEVSEDVLAKNSSYS 600
Query: 1920 MFVEE---WKRLKSQLQQLPSHFPGFLDGE 2000
+++E+ + LK++++ HF FLDGE
Sbjct: 601 IWMEDLRGLRELKAKVKTFSHHFNPFLDGE 630
>emb|CBI21592.3| unnamed protein product [Vitis vinifera]
Length = 629
Score = 649 bits (1675), Expect = 0.0
Identities = 332/626 (53%), Positives = 431/626 (68%), Gaps = 5/626 (0%)
Frame = +3
Query: 138 MDAEVSLFEPSEMLATLLASTPLLEESWKLCGQANSAAPQGYLIKQTGEVCYVAFSGVQI 317
MDAE SLFE SEMLAT ++STP+L++SW+LC AN++A + Q + YVAFSG +
Sbjct: 1 MDAETSLFESSEMLATFISSTPVLQDSWRLCSLANTSA--SVVTDQVRGIAYVAFSGTIM 58
Query: 318 LAGLDPDSQYLVPIERFSNGLFSAL---HREGEGGETAMVHAGLLQLFLSFYANPIFQNQ 488
DP L ++R +GLF L H + + + M+HA +L FLS Y +P F NQ
Sbjct: 59 PPLADPSCANLEALDRPPDGLFPPLQQRHAQHQHEDPPMLHAAILHHFLSLYTSPAFLNQ 118
Query: 489 MMEITSKSKSVVFTGHSVGGTIGSLSALWLLSCLHSISSSFAVICITFGSPMLGNKSLSK 668
++ + KSK+VV TGHS+GG + SLSALWLLS L S SS+ V+CITFGSP+LGN++LS+
Sbjct: 119 ILTVIEKSKAVVMTGHSMGGAVASLSALWLLSHLQSTSSALPVLCITFGSPLLGNEALSR 178
Query: 669 SILQERWGGNFLHVVGQHDIVPRLLFAPSAPLIPHLQYFF-DFWHLYMKSQFSHQPPVQF 845
+IL+ERW GNF HVV HD VPRL AP L +F FWHL M S S +Q
Sbjct: 179 AILRERWAGNFCHVVSNHDFVPRLFLAPLPSLSTQQPHFVRQFWHLLMTSLQSVSETIQ- 237
Query: 846 YKDKGAELFHVVMNSLEALTKGLQDSDQGNLFWPFGSYIFCTSKGSICVDNAVSVIKLLH 1025
LF V+ ++A + + F PFG+Y+F + +G++CV++A + +K+L
Sbjct: 238 -------LFRSVLPFVQASAATTGEGWVKSPFSPFGNYLFFSEEGAVCVNDAAAAVKMLE 290
Query: 1026 LLLAKGSPCSSIEDHLNYENYVSRVSWQILSSNNFLDGNLPESSYEAGITLALQSSDIAP 1205
L+ SP SSIEDHL Y +YV + SWQ+L +F G PESSYEAG+ LA+QS +A
Sbjct: 291 LMFTTASPGSSIEDHLKYGDYVGKASWQLLMRKSFTQGEPPESSYEAGVALAVQSCGLAG 350
Query: 1206 SEPVFGPAKDSLKMAKQMGRTPS-LNSANLAIGLSKITPLRAQIEWYKDTCDDSDDKMGY 1382
E + GPAKD LKMAK++ P LNSANLAI LSK P RAQIEW+K +CD SDD+MGY
Sbjct: 351 QESIAGPAKDCLKMAKRVNPLPPHLNSANLAITLSKNVPYRAQIEWFKASCDKSDDQMGY 410
Query: 1383 YDSFKQRGASKKAFKVNMNRHKLARFWNDVISKLETNQLPHDFHKRSKWVNASQFYKLLV 1562
YDSFK RGASKK K+NMNR LA FW++VI LE+NQLPHDF+KR+KWVNASQFYKLLV
Sbjct: 411 YDSFKLRGASKKGAKINMNRCLLAGFWDNVIYMLESNQLPHDFNKRAKWVNASQFYKLLV 470
Query: 1563 EPLDIAEYYKCGEHLRKGHYMEHGRAKRYKIFDKWWRDREVSANPPNSRSNFASLTQDSC 1742
EPLDIAEYY+ G+H +GHY+++GR KRY+IFD+WW+ RE N R+++ASLTQDSC
Sbjct: 471 EPLDIAEYYRTGKHRTQGHYLKNGREKRYEIFDRWWKGREAGDEENNKRTSYASLTQDSC 530
Query: 1743 FWARVEEARDWLNRVRSEGDVRIQSSLLEKINKFDQYAREMVDRKEVSKDVLAKNSSYNM 1922
FWARVEEA+DWL++VRSE D L + I++F+ YA +V+ KEVS DVLAKNSS+ +
Sbjct: 531 FWARVEEAKDWLDQVRSESDTGRSDMLWQDIDRFESYATRLVENKEVSIDVLAKNSSFTL 590
Query: 1923 FVEEWKRLKSQLQQLPSHFPGFLDGE 2000
+EE + K + QQ P FP F + E
Sbjct: 591 LMEELQDFKKKTQQFPPQFPAFWNEE 616
>ref|XP_002275637.1| PREDICTED: uncharacterized protein LOC100257796 [Vitis vinifera]
Length = 619
Score = 649 bits (1675), Expect = 0.0
Identities = 332/626 (53%), Positives = 431/626 (68%), Gaps = 5/626 (0%)
Frame = +3
Query: 138 MDAEVSLFEPSEMLATLLASTPLLEESWKLCGQANSAAPQGYLIKQTGEVCYVAFSGVQI 317
MDAE SLFE SEMLAT ++STP+L++SW+LC AN++A + Q + YVAFSG +
Sbjct: 1 MDAETSLFESSEMLATFISSTPVLQDSWRLCSLANTSA--SVVTDQVRGIAYVAFSGTIM 58
Query: 318 LAGLDPDSQYLVPIERFSNGLFSAL---HREGEGGETAMVHAGLLQLFLSFYANPIFQNQ 488
DP L ++R +GLF L H + + + M+HA +L FLS Y +P F NQ
Sbjct: 59 PPLADPSCANLEALDRPPDGLFPPLQQRHAQHQHEDPPMLHAAILHHFLSLYTSPAFLNQ 118
Query: 489 MMEITSKSKSVVFTGHSVGGTIGSLSALWLLSCLHSISSSFAVICITFGSPMLGNKSLSK 668
++ + KSK+VV TGHS+GG + SLSALWLLS L S SS+ V+CITFGSP+LGN++LS+
Sbjct: 119 ILTVIEKSKAVVMTGHSMGGAVASLSALWLLSHLQSTSSALPVLCITFGSPLLGNEALSR 178
Query: 669 SILQERWGGNFLHVVGQHDIVPRLLFAPSAPLIPHLQYFF-DFWHLYMKSQFSHQPPVQF 845
+IL+ERW GNF HVV HD VPRL AP L +F FWHL M S S +Q
Sbjct: 179 AILRERWAGNFCHVVSNHDFVPRLFLAPLPSLSTQQPHFVRQFWHLLMTSLQSVSETIQ- 237
Query: 846 YKDKGAELFHVVMNSLEALTKGLQDSDQGNLFWPFGSYIFCTSKGSICVDNAVSVIKLLH 1025
LF V+ ++A + + F PFG+Y+F + +G++CV++A + +K+L
Sbjct: 238 -------LFRSVLPFVQASAATTGEGWVKSPFSPFGNYLFFSEEGAVCVNDAAAAVKMLE 290
Query: 1026 LLLAKGSPCSSIEDHLNYENYVSRVSWQILSSNNFLDGNLPESSYEAGITLALQSSDIAP 1205
L+ SP SSIEDHL Y +YV + SWQ+L +F G PESSYEAG+ LA+QS +A
Sbjct: 291 LMFTTASPGSSIEDHLKYGDYVGKASWQLLMRKSFTQGEPPESSYEAGVALAVQSCGLAG 350
Query: 1206 SEPVFGPAKDSLKMAKQMGRTPS-LNSANLAIGLSKITPLRAQIEWYKDTCDDSDDKMGY 1382
E + GPAKD LKMAK++ P LNSANLAI LSK P RAQIEW+K +CD SDD+MGY
Sbjct: 351 QESIAGPAKDCLKMAKRVNPLPPHLNSANLAITLSKNVPYRAQIEWFKASCDKSDDQMGY 410
Query: 1383 YDSFKQRGASKKAFKVNMNRHKLARFWNDVISKLETNQLPHDFHKRSKWVNASQFYKLLV 1562
YDSFK RGASKK K+NMNR LA FW++VI LE+NQLPHDF+KR+KWVNASQFYKLLV
Sbjct: 411 YDSFKLRGASKKGAKINMNRCLLAGFWDNVIYMLESNQLPHDFNKRAKWVNASQFYKLLV 470
Query: 1563 EPLDIAEYYKCGEHLRKGHYMEHGRAKRYKIFDKWWRDREVSANPPNSRSNFASLTQDSC 1742
EPLDIAEYY+ G+H +GHY+++GR KRY+IFD+WW+ RE N R+++ASLTQDSC
Sbjct: 471 EPLDIAEYYRTGKHRTQGHYLKNGREKRYEIFDRWWKGREAGDEENNKRTSYASLTQDSC 530
Query: 1743 FWARVEEARDWLNRVRSEGDVRIQSSLLEKINKFDQYAREMVDRKEVSKDVLAKNSSYNM 1922
FWARVEEA+DWL++VRSE D L + I++F+ YA +V+ KEVS DVLAKNSS+ +
Sbjct: 531 FWARVEEAKDWLDQVRSESDTGRSDMLWQDIDRFESYATRLVENKEVSIDVLAKNSSFTL 590
Query: 1923 FVEEWKRLKSQLQQLPSHFPGFLDGE 2000
+EE + K + QQ P FP F + E
Sbjct: 591 LMEELQDFKKKTQQFPPQFPAFWNEE 616