BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cephaelis21_contig00005824
         (1096 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002522721.1| cytochrome P450, putative [Ricinus communis]...   405   e-110
ref|XP_003634756.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P...   393   e-107
gb|ACM92061.1| tabersonine 16-hydroxylase CYP71D12 [Catharanthus...   393   e-107
sp|P98183.1|C71DC_CATRO RecName: Full=Tabersonine 16-hydroxylase...   393   e-107
ref|XP_003519623.1| PREDICTED: cytochrome P450 71D10-like [Glyci...   385   e-104

>ref|XP_002522721.1| cytochrome P450, putative [Ricinus communis]
            gi|223537959|gb|EEF39572.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 499

 Score =  405 bits (1040), Expect = e-110
 Identities = 200/363 (55%), Positives = 263/363 (72%)
 Frame = -3

Query: 1094 QLLGPLPHRILADLAKKYGPLMHIQLGXXXXXXXXXXXXXXXVMRDHDIIFANRPPLLAA 915
            QLLG LPH  L DLAKKYG +MH+QLG               VM+ HDI+FA RP LLAA
Sbjct: 48   QLLGSLPHHRLRDLAKKYGSIMHLQLGEVPHVVVSSPEAAKEVMKTHDIVFAQRPFLLAA 107

Query: 914  CVILYNCQDIAFSPYGNYWRQMRKICALELLSSKRVQTFGSTRKEEVMNMIKSISYQKGS 735
             VI YN  DIAFSPY +YWRQ+RKIC LELLS+KRVQ+F S R+EEV N+I +++   G 
Sbjct: 108  SVITYNFTDIAFSPYSDYWRQLRKICVLELLSAKRVQSFRSIREEEVSNLINTLTSFSGK 167

Query: 734  VVNLSKMIYTLSYSIIAKAAFGKSCKYTEEYMSIIEVLTKVFGGFSLVDLYPSVKIIERI 555
              N S+ +++ +Y I A+A+FGK  K  EE++ + + +T+V GGFSL DL+PS+  +  I
Sbjct: 168  PFNFSRKLFSSTYGIAARASFGKKYKDQEEFIKVAQQMTEVAGGFSLADLFPSIAFLHVI 227

Query: 554  TGTRNKIERVREQNDVILESILTEHKNRRAESKPVNQEAKEDLVDVLLNVQESGEFGAPL 375
            +G ++++ R+R+  D ILE+I+ +H+ R    K   +   +DLVDVLL  Q+ G+   PL
Sbjct: 228  SGMKSRLLRLRDDGDNILENIIDDHRARNMTGKTSGEGEDDDLVDVLLRFQKGGQLEFPL 287

Query: 374  TNNSIKAVIHDIFSAGGETSATTVIWAMFEMIKNPTMLERAQDEVRKVFGGKGNVNESQL 195
            T N+IKAVI DIF AG ETS+TTV WAM EM++NP ++E+AQ+EVR+VFG KGNV+E++L
Sbjct: 288  TTNNIKAVILDIFIAGSETSSTTVEWAMSEMLRNPRVMEKAQEEVRRVFGKKGNVDEAEL 347

Query: 194  HELKYLQAVIKESLRLHPAVPLLLPRESSEQCEINGYVIPSKTRVMVNCWAIGRDPMHWT 15
            HEL YL+ VIKE+LRLHP  PLLLPRES E CEINGY IP  ++V+VN WAIGRDP +WT
Sbjct: 348  HELNYLKLVIKETLRLHPPAPLLLPRESRESCEINGYYIPVNSKVIVNAWAIGRDPNNWT 407

Query: 14   DPE 6
            + E
Sbjct: 408  EAE 410


>ref|XP_003634756.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 71D10-like [Vitis
            vinifera]
          Length = 554

 Score =  393 bits (1009), Expect = e-107
 Identities = 187/364 (51%), Positives = 263/364 (72%)
 Frame = -3

Query: 1094 QLLGPLPHRILADLAKKYGPLMHIQLGXXXXXXXXXXXXXXXVMRDHDIIFANRPPLLAA 915
            QL+G LPH  L  LA KYGP MH++LG               VM+ HD IFA RPPLL++
Sbjct: 98   QLVGSLPHHTLKRLASKYGPFMHLELGEVSALVVSSPEIAREVMKTHDTIFAQRPPLLSS 157

Query: 914  CVILYNCQDIAFSPYGNYWRQMRKICALELLSSKRVQTFGSTRKEEVMNMIKSISYQKGS 735
             +I YN   I+FSPYG+YWRQ+RKIC +ELLS+KRV++F S R+ EV  +I SIS   GS
Sbjct: 158  TIINYNATSISFSPYGDYWRQLRKICTIELLSAKRVKSFQSIREXEVSKLIWSISLNAGS 217

Query: 734  VVNLSKMIYTLSYSIIAKAAFGKSCKYTEEYMSIIEVLTKVFGGFSLVDLYPSVKIIERI 555
             +NLS+ I++L+Y I +++AFGK  +  + ++S I    ++  GF + D+YPS+K +  I
Sbjct: 218  PINLSEKIFSLTYGITSRSAFGKKFRGQDAFVSAILEAVELSAGFCVADMYPSLKWLHYI 277

Query: 554  TGTRNKIERVREQNDVILESILTEHKNRRAESKPVNQEAKEDLVDVLLNVQESGEFGAPL 375
            +G + K+E+V ++ D IL +I+ +H+ R+  +K    E +EDLVDVLLN+QE G+ G PL
Sbjct: 278  SGMKPKLEKVHQKIDRILNNIIDDHRKRKTTTKAGQPETQEDLVDVLLNLQEHGDLGIPL 337

Query: 374  TNNSIKAVIHDIFSAGGETSATTVIWAMFEMIKNPTMLERAQDEVRKVFGGKGNVNESQL 195
            T+ ++KAV+ DIFS GGETS+T V+WAM EM+K+P ++E+AQ EVR+VF GK ++NE+ +
Sbjct: 338  TDGNVKAVLLDIFSGGGETSSTAVVWAMAEMLKSPIVMEKAQAEVRRVFDGKRDINETGI 397

Query: 194  HELKYLQAVIKESLRLHPAVPLLLPRESSEQCEINGYVIPSKTRVMVNCWAIGRDPMHWT 15
            HELKYL +V+KE+LRLHP+VPLLLPRE  E+C INGY IP  T+V++N WAI +DP HW 
Sbjct: 398  HELKYLNSVVKETLRLHPSVPLLLPRECRERCVINGYEIPENTKVIINAWAIAQDPDHWF 457

Query: 14   DPER 3
            +P +
Sbjct: 458  EPNK 461


>gb|ACM92061.1| tabersonine 16-hydroxylase CYP71D12 [Catharanthus roseus]
            gi|325989357|gb|ADZ48683.1| tabersonine 16-hydroxylase
            [synthetic construct]
          Length = 506

 Score =  393 bits (1009), Expect = e-107
 Identities = 201/368 (54%), Positives = 271/368 (73%), Gaps = 4/368 (1%)
 Frame = -3

Query: 1094 QLLGPLPHRILADLAKKYGPLMHIQLGXXXXXXXXXXXXXXXVMRDHDIIFANRPPLLAA 915
            QL G   H IL DLAKKYGPLMH+++G               + R HDI+FA+RP  L +
Sbjct: 52   QLSGGHTHHILRDLAKKYGPLMHLKIGEVSTIVASSPQIAEEIFRTHDILFADRPSNLES 111

Query: 914  C-VILYNCQDIAFSPYGNYWRQMRKICALELLSSKRVQTFGSTRKEEVMNMIKSISYQKG 738
              ++ Y+  D+  SPYGNYWRQ+RKI  +ELLS K VQ+F S R+EEV+N IKSI  ++G
Sbjct: 112  FKIVSYDFSDMVVSPYGNYWRQLRKISMMELLSQKSVQSFRSIREEEVLNFIKSIGSKEG 171

Query: 737  SVVNLSKMIYTLSYSIIAKAAFGKSCKYTEEYMSIIEVLTKVFGGFSLVDLYPSVKIIER 558
            + +NLSK I  L Y I  +AAFG+  K TEE++ +++ LTK     ++ D++PS+K ++ 
Sbjct: 172  TRINLSKEISLLIYGITTRAAFGEKNKNTEEFIRLLDQLTKAVAEPNIADMFPSLKFLQL 231

Query: 557  ITGTRNKIERVREQNDVILESILTEHKNRRAESKPVNQE---AKEDLVDVLLNVQESGEF 387
            I+ ++ KIE++ +Q DVI+E+IL  HK +   +KP++QE    KEDLVDVLLN+Q   +F
Sbjct: 232  ISTSKYKIEKIHKQFDVIVETILKGHKEKI--NKPLSQENGEKKEDLVDVLLNIQRRNDF 289

Query: 386  GAPLTNNSIKAVIHDIFSAGGETSATTVIWAMFEMIKNPTMLERAQDEVRKVFGGKGNVN 207
             APL + +IKA+I +IFSAG ETS+TTV WAM EMIKNPT++++AQ+EVRKVF  +GNV+
Sbjct: 290  EAPLGDKNIKAIIFNIFSAGTETSSTTVDWAMCEMIKNPTVMKKAQEEVRKVFNEEGNVD 349

Query: 206  ESQLHELKYLQAVIKESLRLHPAVPLLLPRESSEQCEINGYVIPSKTRVMVNCWAIGRDP 27
            E++LH+LKYLQAVIKE+LRLHP VPLLLPRE  EQC+I GY IPSK+RV+VN WAIGRDP
Sbjct: 350  ETKLHQLKYLQAVIKETLRLHPPVPLLLPRECREQCKIKGYTIPSKSRVIVNAWAIGRDP 409

Query: 26   MHWTDPER 3
             +W +PE+
Sbjct: 410  NYWIEPEK 417


>sp|P98183.1|C71DC_CATRO RecName: Full=Tabersonine 16-hydroxylase; AltName: Full=Cytochrome
            P450 71D12 gi|5921278|emb|CAB56503.1| cytochrome P450
            [Catharanthus roseus]
          Length = 495

 Score =  393 bits (1009), Expect = e-107
 Identities = 201/368 (54%), Positives = 271/368 (73%), Gaps = 4/368 (1%)
 Frame = -3

Query: 1094 QLLGPLPHRILADLAKKYGPLMHIQLGXXXXXXXXXXXXXXXVMRDHDIIFANRPPLLAA 915
            QL G   H IL DLAKKYGPLMH+++G               + R HDI+FA+RP  L +
Sbjct: 41   QLSGGHTHHILRDLAKKYGPLMHLKIGEVSTIVASSPQIAEEIFRTHDILFADRPSNLES 100

Query: 914  C-VILYNCQDIAFSPYGNYWRQMRKICALELLSSKRVQTFGSTRKEEVMNMIKSISYQKG 738
              ++ Y+  D+  SPYGNYWRQ+RKI  +ELLS K VQ+F S R+EEV+N IKSI  ++G
Sbjct: 101  FKIVSYDFSDMVVSPYGNYWRQLRKISMMELLSQKSVQSFRSIREEEVLNFIKSIGSKEG 160

Query: 737  SVVNLSKMIYTLSYSIIAKAAFGKSCKYTEEYMSIIEVLTKVFGGFSLVDLYPSVKIIER 558
            + +NLSK I  L Y I  +AAFG+  K TEE++ +++ LTK     ++ D++PS+K ++ 
Sbjct: 161  TRINLSKEISLLIYGITTRAAFGEKNKNTEEFIRLLDQLTKAVAEPNIADMFPSLKFLQL 220

Query: 557  ITGTRNKIERVREQNDVILESILTEHKNRRAESKPVNQE---AKEDLVDVLLNVQESGEF 387
            I+ ++ KIE++ +Q DVI+E+IL  HK +   +KP++QE    KEDLVDVLLN+Q   +F
Sbjct: 221  ISTSKYKIEKIHKQFDVIVETILKGHKEKI--NKPLSQENGEKKEDLVDVLLNIQRRNDF 278

Query: 386  GAPLTNNSIKAVIHDIFSAGGETSATTVIWAMFEMIKNPTMLERAQDEVRKVFGGKGNVN 207
             APL + +IKA+I +IFSAG ETS+TTV WAM EMIKNPT++++AQ+EVRKVF  +GNV+
Sbjct: 279  EAPLGDKNIKAIIFNIFSAGTETSSTTVDWAMCEMIKNPTVMKKAQEEVRKVFNEEGNVD 338

Query: 206  ESQLHELKYLQAVIKESLRLHPAVPLLLPRESSEQCEINGYVIPSKTRVMVNCWAIGRDP 27
            E++LH+LKYLQAVIKE+LRLHP VPLLLPRE  EQC+I GY IPSK+RV+VN WAIGRDP
Sbjct: 339  ETKLHQLKYLQAVIKETLRLHPPVPLLLPRECREQCKIKGYTIPSKSRVIVNAWAIGRDP 398

Query: 26   MHWTDPER 3
             +W +PE+
Sbjct: 399  NYWIEPEK 406


>ref|XP_003519623.1| PREDICTED: cytochrome P450 71D10-like [Glycine max]
          Length = 508

 Score =  385 bits (988), Expect = e-104
 Identities = 189/363 (52%), Positives = 265/363 (73%), Gaps = 1/363 (0%)
 Frame = -3

Query: 1088 LGPLPHRILADLAKKYGPLMHIQLGXXXXXXXXXXXXXXXVMRDHDIIFANRPPLLAACV 909
            LG LPHR LA LA +YGPLMH+QLG               VM+ HDIIFANRP +LAA V
Sbjct: 55   LGTLPHRSLARLANQYGPLMHMQLGELSCIMVSSPEMAKEVMKTHDIIFANRPYVLAADV 114

Query: 908  ILYNCQDIAFSPYGNYWRQMRKICALELLSSKRVQTFGSTRKEEVMNMIKSISYQKGSVV 729
            I Y  + + FSP G YWRQMRKIC +ELL+ KRV +F S R++E+   +K +S  +GS +
Sbjct: 115  ITYGSKGMTFSPQGTYWRQMRKICTMELLAPKRVDSFRSIREQELSIFVKEMSLSEGSPI 174

Query: 728  NLSKMIYTLSYSIIAKAAFGKSCKYTEEYMSIIEVLTKVFGGFSLVDLYPSVKIIERITG 549
            NLS+ I +L+Y +I++ AFGK  K  E Y+  ++ +T    GFSL DLYPS+ +++ +TG
Sbjct: 175  NLSEKISSLAYGLISRIAFGKKSKDQEAYIEFMKGVTDTVSGFSLADLYPSIGLLQVLTG 234

Query: 548  TRNKIERVREQNDVILESILTEHKNRRAESKPV-NQEAKEDLVDVLLNVQESGEFGAPLT 372
             R ++E++R   D I+++I+ +H+++ ++++PV  +E  EDLVDVLL +Q++G    PL+
Sbjct: 235  IRPRVEKIRRGMDRIIDNIVRDHRDKNSDTQPVVGEENGEDLVDVLLRLQKNGNLQHPLS 294

Query: 371  NNSIKAVIHDIFSAGGETSATTVIWAMFEMIKNPTMLERAQDEVRKVFGGKGNVNESQLH 192
            +  +KA I DIFSAG ET++TT+ WAM E++KNP M+E+AQ EVR+VF  KG V+E+ +H
Sbjct: 295  DTVVKATIMDIFSAGSETTSTTMEWAMSELVKNPRMMEKAQIEVRRVFDPKGYVDETSIH 354

Query: 191  ELKYLQAVIKESLRLHPAVPLLLPRESSEQCEINGYVIPSKTRVMVNCWAIGRDPMHWTD 12
            ELKYL++VIKE+LRLH  VPLLLPRE SE+CEINGY IP+K++V+VN WAIGRDP +W +
Sbjct: 355  ELKYLRSVIKETLRLHTPVPLLLPRECSERCEINGYEIPAKSKVIVNAWAIGRDPNYWIE 414

Query: 11   PER 3
             E+
Sbjct: 415  AEK 417