BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cephaelis21_contig00004451
         (2101 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADU19849.1| putative secologanin synthase [Camptotheca acumin...   510   e-159
dbj|BAL45206.1| cytochrome P450 monooxygenase [Glycyrrhiza urale...   492   e-153
gb|ABC59078.1| cytochrome P450 monooxygenase CYP72A59 [Medicago ...   492   e-152
gb|ABC69417.1| CYP72A54 [Nicotiana tabacum]                           501   e-151
sp|Q05047.1|C72A1_CATRO RecName: Full=Secologanin synthase; Shor...   481   e-151

>gb|ADU19849.1| putative secologanin synthase [Camptotheca acuminata]
          Length = 524

 Score =  510 bits (1313), Expect(2) = e-159
 Identities = 245/440 (55%), Positives = 323/440 (73%), Gaps = 1/440 (0%)
 Frame = +2

Query: 518  GRIPRVFVTDPELIREVLTKYYKFHKNHHDLDPITKFLLTGIGSLEGEPWSRRRKIINPA 697
            GR+PRV + +P+LI+++L  +  F KNHH  +P+TKFLLTGIGSLEG+ W++ R+II+P+
Sbjct: 104  GRMPRVHIMEPDLIKDILANHNDFMKNHHAYNPLTKFLLTGIGSLEGDKWAKHRRIISPS 163

Query: 698  FHFEKLKXXXXXXXXXXXXXXXXXEKLKLMLPAFYMACDDMVRKWDAKVPDGGSAEVEVW 877
            FH EKLK                      MLPAFY++ DD++ KW+ +    GS E++++
Sbjct: 164  FHLEKLKT---------------------MLPAFYVSYDDLLTKWEQQCSSKGSVEIDLF 202

Query: 878  HDIETLTGDVISRTLFGSNYEEGRRIFELMRELTALTIDVIRSVYIPGMRFVPTKRNNRL 1057
               +TLT DVISR  FGS+Y EG RIF L++EL  LT+DV+RSVY+PG  F+PTKRNNR+
Sbjct: 203  PTFDTLTSDVISRVAFGSSYGEGGRIFILLKELMDLTVDVMRSVYVPGSSFLPTKRNNRM 262

Query: 1058 RAIDKEVRGRISEIITKKMKSMKAGGGTAEDFLGILLECNLKEIEEQGNK-SAGMSTEEI 1234
            R +D E++ R+S II  ++K+MKAG  + ED LG LLE N KEIE  GNK +AGMS E++
Sbjct: 263  REVDGEIKDRLSGIINSRVKAMKAGEPSGEDLLGTLLESNFKEIERLGNKKNAGMSIEDV 322

Query: 1235 IGECKLFYFAGQDTTSTLLVWTMFFLSRYPEWQQRAREEVFQVFGDRKPDYDGISRLKIV 1414
            I ECKLFYFAGQ+TT  LL WT   LSR+PEWQ+RAREE+FQVFG+ K D+D +  LKIV
Sbjct: 323  ISECKLFYFAGQETTGILLTWTCVLLSRHPEWQERAREEIFQVFGNGKVDFDRVQNLKIV 382

Query: 1415 TMILNEVLRIYTPVAELTKVAHDATQLGKYFIPAGVQLMMPQMLMHHDPEIWGDDVMEFN 1594
             MIL EVLR+Y PV ELTKV ++  +LG   IPAGVQLMMP +L+H D E+WG D  EFN
Sbjct: 383  PMILYEVLRLYPPVIELTKVTYEEQKLGNLTIPAGVQLMMPSILLHRDQEMWGADSKEFN 442

Query: 1595 PERFSEGVLKATKSQGSYFPFSLGPRMCIGMNFALLEAKMAMALVLRRFSFKLSPSYVHA 1774
            P RF++G+ KA KS   Y PFS GPR+C+G NFALL+AKMA+ ++L+RF+F LSP+Y HA
Sbjct: 443  PGRFADGISKAVKSPFFYIPFSWGPRICVGQNFALLQAKMALTMILQRFTFDLSPTYAHA 502

Query: 1775 PMTLITLQPQFGTHLIMQKL 1834
            P T++TLQPQ G  ++ +K+
Sbjct: 503  PFTVLTLQPQHGAQVVFRKI 522



 Score = 82.0 bits (201), Expect(2) = e-159
 Identities = 33/61 (54%), Positives = 48/61 (78%)
 Frame = +1

Query: 265 LREQGFKGNPYRLFLGDQYESGKLIREALSKPIAVDEDVKKRIIPHILKAVETHGKKSFM 444
           LR+QGFKGN YRL +GD  ES  +++E +SKPI V ED+ +R++PH++K ++T+GK SF 
Sbjct: 42  LRKQGFKGNSYRLLVGDLKESSMMLKETMSKPINVSEDIVERVMPHVIKTIDTYGKNSFT 101

Query: 445 W 447
           W
Sbjct: 102 W 102


>dbj|BAL45206.1| cytochrome P450 monooxygenase [Glycyrrhiza uralensis]
          Length = 522

 Score =  492 bits (1267), Expect(3) = e-153
 Identities = 238/440 (54%), Positives = 317/440 (72%), Gaps = 1/440 (0%)
 Frame = +2

Query: 518  GRIPRVFVTDPELIREVLTKYYKFHKNHHDLDPITKFLLTGIGSLEGEPWSRRRKIINPA 697
            G IPRV +TDPE I++VL K Y F K+  D D + K L TG+ S EGE WS+ R+IINPA
Sbjct: 104  GPIPRVTLTDPEQIKDVLNKIYDFPKS--DGDRLVKLLATGLVSYEGEKWSKHRRIINPA 161

Query: 698  FHFEKLKXXXXXXXXXXXXXXXXXEKLKLMLPAFYMACDDMVRKWDAKVPDGGSAEVEVW 877
            F+ EKLK                     +MLP F+ +C+D++ KW+  +   GS E+++W
Sbjct: 162  FNLEKLK---------------------IMLPIFFQSCNDLITKWEGMLSSDGSCEMDIW 200

Query: 878  HDIETLTGDVISRTLFGSNYEEGRRIFELMRELTALTIDVIRSVYIPGMRFVPTKRNNRL 1057
              ++ L  DVI+R  FGS+YEEGRRIF+L +E    T+ V+  VYIPG RF+PT  + R+
Sbjct: 201  PFLQNLASDVIARAAFGSSYEEGRRIFQLQKEQVEHTMSVLVKVYIPGWRFLPTTTHRRM 260

Query: 1058 RAIDKEVRGRISEIITKKMKSMKAGGGTAEDFLGILLECNLKEIEEQGN-KSAGMSTEEI 1234
            + ID+E++  + E+I K+ +++KAG  T  + L ILLE N KE+EE GN ++ GMS E++
Sbjct: 261  KKIDREIKASLKEMINKRERALKAGEATKNNLLDILLESNHKEVEEHGNNRNVGMSLEDV 320

Query: 1235 IGECKLFYFAGQDTTSTLLVWTMFFLSRYPEWQQRAREEVFQVFGDRKPDYDGISRLKIV 1414
            I ECKLFYFAGQ+TTS LLVWTM  LSRYP+WQ RAREEV QVFG++KPD+DG+S LKIV
Sbjct: 321  IEECKLFYFAGQETTSGLLVWTMVLLSRYPDWQARAREEVLQVFGNKKPDFDGLSHLKIV 380

Query: 1415 TMILNEVLRIYTPVAELTKVAHDATQLGKYFIPAGVQLMMPQMLMHHDPEIWGDDVMEFN 1594
            TMIL EVLR+Y PV  L +  H   +LG   +PAGVQL +P +L HHD E+WGDD   FN
Sbjct: 381  TMILYEVLRLYPPVIALARTVHKDVKLGNRTLPAGVQLYLPIVLFHHDRELWGDDAEVFN 440

Query: 1595 PERFSEGVLKATKSQGSYFPFSLGPRMCIGMNFALLEAKMAMALVLRRFSFKLSPSYVHA 1774
            PERFSEGVLKAT  + S+FPF  GPR+C+G NF+LLEAKMA++++L+ FSF+LSP+Y HA
Sbjct: 441  PERFSEGVLKATNGKVSFFPFGWGPRICVGQNFSLLEAKMAVSMILQHFSFELSPAYAHA 500

Query: 1775 PMTLITLQPQFGTHLIMQKL 1834
            P T+ITL+PQ+G H+I++K+
Sbjct: 501  PTTVITLRPQYGAHIILRKV 520



 Score = 68.9 bits (167), Expect(3) = e-153
 Identities = 29/61 (47%), Positives = 44/61 (72%)
 Frame = +1

Query: 265 LREQGFKGNPYRLFLGDQYESGKLIREALSKPIAVDEDVKKRIIPHILKAVETHGKKSFM 444
           LREQG +GNPYRL +GD  +  K+ +EA SKP+ + +D+  R+ P++ ++V  HGK SF+
Sbjct: 42  LREQGLQGNPYRLIVGDLKDLMKMRKEAKSKPMNLSDDILPRVFPYVQQSVTKHGKNSFI 101

Query: 445 W 447
           W
Sbjct: 102 W 102



 Score = 32.3 bits (72), Expect(3) = e-153
 Identities = 12/22 (54%), Positives = 18/22 (81%)
 Frame = +2

Query: 122 LAVYWFYRVVDLYWIRPKKLEK 187
           LAV W +R+++  W+RPKKLE+
Sbjct: 19  LAVTWAWRMLNWLWLRPKKLER 40


>gb|ABC59078.1| cytochrome P450 monooxygenase CYP72A59 [Medicago truncatula]
          Length = 518

 Score =  492 bits (1267), Expect(3) = e-152
 Identities = 237/440 (53%), Positives = 314/440 (71%), Gaps = 1/440 (0%)
 Frame = +2

Query: 518  GRIPRVFVTDPELIREVLTKYYKFHKNHHDLDPITKFLLTGIGSLEGEPWSRRRKIINPA 697
            G  PRV +TDP+ I++VL K Y F K +   +P+ + L  G+ SLEGE WS+ RKIINPA
Sbjct: 100  GPTPRVNITDPDQIKDVLNKIYDFQKPN--ANPLVRLLANGLVSLEGEQWSKHRKIINPA 157

Query: 698  FHFEKLKXXXXXXXXXXXXXXXXXEKLKLMLPAFYMACDDMVRKWDAKVPDGGSAEVEVW 877
            FH EKLK                     +MLP F+ +CDD++ KW+  +   GS E++VW
Sbjct: 158  FHLEKLK---------------------IMLPIFHKSCDDLISKWEKMLSSDGSCEMDVW 196

Query: 878  HDIETLTGDVISRTLFGSNYEEGRRIFELMRELTALTIDVIRSVYIPGMRFVPTKRNNRL 1057
              ++    DVISR  FGS+YEEG+RIF+L  E   LT  ++  VYIPG RF+PT    R+
Sbjct: 197  PFLQNFASDVISRAAFGSSYEEGKRIFQLQTEQAELTTKIMMKVYIPGWRFLPTATPRRM 256

Query: 1058 RAIDKEVRGRISEIITKKMKSMKAGGGTAEDFLGILLECNLKEIEEQGN-KSAGMSTEEI 1234
            + ID++++  ++++I  + +++KAG  T +D LGILLE N KE+EE GN K  GMS +++
Sbjct: 257  KEIDRDIKASLTDMINNRERALKAGEATKDDLLGILLESNHKEMEEHGNNKDVGMSLDDV 316

Query: 1235 IGECKLFYFAGQDTTSTLLVWTMFFLSRYPEWQQRAREEVFQVFGDRKPDYDGISRLKIV 1414
            I ECKLFYFAGQ+TTS LLVWTM  LSRYP+WQ RAREEV QVFG+ KPD+DG+S LKIV
Sbjct: 317  IEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQARAREEVLQVFGNNKPDFDGLSHLKIV 376

Query: 1415 TMILNEVLRIYTPVAELTKVAHDATQLGKYFIPAGVQLMMPQMLMHHDPEIWGDDVMEFN 1594
            TMIL EVLR+Y PV  L +  H   +LG   +PAGVQ+ +  +++HHD E+WG+D   FN
Sbjct: 377  TMILYEVLRLYPPVIALARRVHKDVKLGNLALPAGVQVFLSIIMVHHDIELWGEDAKVFN 436

Query: 1595 PERFSEGVLKATKSQGSYFPFSLGPRMCIGMNFALLEAKMAMALVLRRFSFKLSPSYVHA 1774
            PERFSEGVLKAT  + S+FPF+ GPR+CIG NF++LEAKMA+A++L+RFSF+LSPSY HA
Sbjct: 437  PERFSEGVLKATNGRNSFFPFAGGPRICIGQNFSMLEAKMAIAMILQRFSFELSPSYAHA 496

Query: 1775 PMTLITLQPQFGTHLIMQKL 1834
            P T+ITLQPQ+G H+I+ KL
Sbjct: 497  PATVITLQPQYGAHIILHKL 516



 Score = 63.2 bits (152), Expect(3) = e-152
 Identities = 28/61 (45%), Positives = 41/61 (67%)
 Frame = +1

Query: 265 LREQGFKGNPYRLFLGDQYESGKLIREALSKPIAVDEDVKKRIIPHILKAVETHGKKSFM 444
           LREQG KGN YRL +GD  +  K+ +EA SKP+ + +D+  R+   + ++V  HGK SF+
Sbjct: 38  LREQGLKGNSYRLLVGDMNDLLKMRKEATSKPMNLSDDIVPRVYSFVHQSVTKHGKNSFI 97

Query: 445 W 447
           W
Sbjct: 98  W 98



 Score = 33.1 bits (74), Expect(3) = e-152
 Identities = 12/30 (40%), Positives = 22/30 (73%)
 Frame = +2

Query: 98  IAVFGSVGLAVYWFYRVVDLYWIRPKKLEK 187
           I +F  + L++ W +R+++  W++PKKLEK
Sbjct: 7   IILFTLMTLSLIWAWRILNWLWLKPKKLEK 36


>gb|ABC69417.1| CYP72A54 [Nicotiana tabacum]
          Length = 517

 Score =  501 bits (1291), Expect(3) = e-151
 Identities = 245/439 (55%), Positives = 319/439 (72%)
 Frame = +2

Query: 518  GRIPRVFVTDPELIREVLTKYYKFHKNHHDLDPITKFLLTGIGSLEGEPWSRRRKIINPA 697
            G  P +F+TDPELIRE+L+K Y + +   +  PITK L  G+ S E E W++ RKIINPA
Sbjct: 103  GPKPTMFITDPELIREILSKSYIYQEIQGN--PITKLLAQGLVSYEAEKWAKHRKIINPA 160

Query: 698  FHFEKLKXXXXXXXXXXXXXXXXXEKLKLMLPAFYMACDDMVRKWDAKVPDGGSAEVEVW 877
            FH +KLK                      MLP+FY++C DM+RKW++     GS E++VW
Sbjct: 161  FHLDKLKH---------------------MLPSFYLSCCDMLRKWESIASSEGS-EIDVW 198

Query: 878  HDIETLTGDVISRTLFGSNYEEGRRIFELMRELTALTIDVIRSVYIPGMRFVPTKRNNRL 1057
              +ETLT D ISRT FGSNYE+GR+IFEL +E   L +   R +YIPG RFVPTKRN R+
Sbjct: 199  PFLETLTSDAISRTAFGSNYEDGRQIFELQKEQAELILQAARWLYIPGWRFVPTKRNKRM 258

Query: 1058 RAIDKEVRGRISEIITKKMKSMKAGGGTAEDFLGILLECNLKEIEEQGNKSAGMSTEEII 1237
            + I KEVR  +  II K+++ MKAG    +D LGILLE N KEI+  GNK+ GM+ +E+I
Sbjct: 259  KQIAKEVRSLVLGIINKRIREMKAGEAAKDDLLGILLESNFKEIQMHGNKNFGMTIDEVI 318

Query: 1238 GECKLFYFAGQDTTSTLLVWTMFFLSRYPEWQQRAREEVFQVFGDRKPDYDGISRLKIVT 1417
             ECKLFYFAGQ+TTS LLVWT+  LS++ +WQ+RAREEV QVFG  KPDYD +++LK+VT
Sbjct: 319  EECKLFYFAGQETTSVLLVWTLILLSKHVDWQERAREEVHQVFGSNKPDYDALNQLKVVT 378

Query: 1418 MILNEVLRIYTPVAELTKVAHDATQLGKYFIPAGVQLMMPQMLMHHDPEIWGDDVMEFNP 1597
            MI NEVLR+Y P   +++  H+ T+LG   +PAG+QL++P + +HHD EIWGDD  EF P
Sbjct: 379  MIFNEVLRLYPPGITISRTVHEDTKLGNLSLPAGIQLVLPAIWLHHDNEIWGDDAKEFKP 438

Query: 1598 ERFSEGVLKATKSQGSYFPFSLGPRMCIGMNFALLEAKMAMALVLRRFSFKLSPSYVHAP 1777
            ERFSEGV KATK + +YFPFS GPR+C+G+NFA+LEAKMA+AL+L+ ++F+LSPSY HAP
Sbjct: 439  ERFSEGVNKATKGKFAYFPFSWGPRICVGLNFAMLEAKMALALILQHYAFELSPSYAHAP 498

Query: 1778 MTLITLQPQFGTHLIMQKL 1834
             T+ITLQPQ G  LI++KL
Sbjct: 499  HTIITLQPQHGAPLILRKL 517



 Score = 59.3 bits (142), Expect(3) = e-151
 Identities = 25/61 (40%), Positives = 37/61 (60%)
 Frame = +1

Query: 265 LREQGFKGNPYRLFLGDQYESGKLIREALSKPIAVDEDVKKRIIPHILKAVETHGKKSFM 444
           L+ +GFKG+ Y+L  GD  E   ++ EA +KP+    D   R++PH  K +  +GK SFM
Sbjct: 41  LKMEGFKGSSYKLLFGDMKEINTMVEEAKTKPMNFTNDYVARVLPHFTKLMLQYGKNSFM 100

Query: 445 W 447
           W
Sbjct: 101 W 101



 Score = 26.6 bits (57), Expect(3) = e-151
 Identities = 8/18 (44%), Positives = 14/18 (77%)
 Frame = +2

Query: 134 WFYRVVDLYWIRPKKLEK 187
           W ++ ++  WI+PKK+EK
Sbjct: 22  WVWKFLNWVWIQPKKMEK 39


>sp|Q05047.1|C72A1_CATRO RecName: Full=Secologanin synthase; Short=SLS; AltName:
            Full=CYPLXXII; AltName: Full=Cytochrome P450 72A1
            gi|167484|gb|AAA33106.1| cytochrome P-450 protein
            [Catharanthus roseus] gi|445604|prf||1909351A cytochrome
            P450
          Length = 524

 Score =  481 bits (1239), Expect(3) = e-151
 Identities = 232/440 (52%), Positives = 312/440 (70%), Gaps = 1/440 (0%)
 Frame = +2

Query: 518  GRIPRVFVTDPELIREVLTKYYKFHKNHHDLDPITKFLLTGIGSLEGEPWSRRRKIINPA 697
            GRIPR+ V +PELI+EVLT   K+ KN    +P+ KFLLTG+GS EG  WS+ R+II+PA
Sbjct: 104  GRIPRIHVMEPELIKEVLTHSSKYQKNFDVHNPLVKFLLTGVGSFEGAKWSKHRRIISPA 163

Query: 698  FHFEKLKXXXXXXXXXXXXXXXXXEKLKLMLPAFYMACDDMVRKWDAKVPDGGSAEVEVW 877
            F  EKLK                      MLPAF +   DM+ KW+      GS EV+++
Sbjct: 164  FTLEKLKS---------------------MLPAFAICYHDMLTKWEKIAEKQGSHEVDIF 202

Query: 878  HDIETLTGDVISRTLFGSNYEEGRRIFELMRELTALTIDVIRSVYIPGMRFVPTKRNNRL 1057
               + LT DVIS+  FGS YEEG +IF L++EL  LTID +R VYIPG  ++PTKRN R+
Sbjct: 203  PTFDVLTSDVISKVAFGSTYEEGGKIFRLLKELMDLTIDCMRDVYIPGWSYLPTKRNKRM 262

Query: 1058 RAIDKEVRGRISEIITKKMKSMKAGGGTAEDFLGILLECNLKEIEEQGNKS-AGMSTEEI 1234
            + I+KE+   +  II K+MK++KAG    +D LG+LLE N++EI++QGNK   GMS  ++
Sbjct: 263  KEINKEITDMLRFIINKRMKALKAGEPGEDDLLGVLLESNIQEIQKQGNKKDGGMSINDV 322

Query: 1235 IGECKLFYFAGQDTTSTLLVWTMFFLSRYPEWQQRAREEVFQVFGDRKPDYDGISRLKIV 1414
            I ECKLFYFAGQ+TT  LL WT   LS++PEWQ+RAREEV Q FG  KP+++ ++ LK V
Sbjct: 323  IEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREEVLQAFGKNKPEFERLNHLKYV 382

Query: 1415 TMILNEVLRIYTPVAELTKVAHDATQLGKYFIPAGVQLMMPQMLMHHDPEIWGDDVMEFN 1594
            +MIL EVLR+Y PV +LTK+ H  T+LG Y IPAG Q+M+P +++H +  IWG+D MEFN
Sbjct: 383  SMILYEVLRLYPPVIDLTKIVHKDTKLGSYTIPAGTQVMLPTVMLHREKSIWGEDAMEFN 442

Query: 1595 PERFSEGVLKATKSQGSYFPFSLGPRMCIGMNFALLEAKMAMALVLRRFSFKLSPSYVHA 1774
            P RF +GV  ATK+  +Y PFS GPR+C+G NFALL+AK+ +A++L+RF F ++PSYVHA
Sbjct: 443  PMRFVDGVANATKNNVTYLPFSWGPRVCLGQNFALLQAKLGLAMILQRFKFDVAPSYVHA 502

Query: 1775 PMTLITLQPQFGTHLIMQKL 1834
            P T++T+QPQFG+H+I +KL
Sbjct: 503  PFTILTVQPQFGSHVIYKKL 522



 Score = 76.3 bits (186), Expect(3) = e-151
 Identities = 32/61 (52%), Positives = 45/61 (73%)
 Frame = +1

Query: 265 LREQGFKGNPYRLFLGDQYESGKLIREALSKPIAVDEDVKKRIIPHILKAVETHGKKSFM 444
           LR+QGF+GNPYR  +GD  ESGK+ +EALSKP+  + D+  R++PHI   + T+G+ SF 
Sbjct: 42  LRQQGFRGNPYRFLVGDVKESGKMHQEALSKPMEFNNDIVPRLMPHINHTINTYGRNSFT 101

Query: 445 W 447
           W
Sbjct: 102 W 102



 Score = 28.5 bits (62), Expect(3) = e-151
 Identities = 10/22 (45%), Positives = 15/22 (68%)
 Frame = +2

Query: 122 LAVYWFYRVVDLYWIRPKKLEK 187
           L + W +RV+D  W  PK++EK
Sbjct: 19  LVMAWAWRVLDWAWFTPKRIEK 40