BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cephaelis21_contig00004292
         (924 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACM92061.1| tabersonine 16-hydroxylase CYP71D12 [Catharanthus...   315   1e-83
sp|P98183.1|C71DC_CATRO RecName: Full=Tabersonine 16-hydroxylase...   315   1e-83
ref|XP_003634756.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P...   315   1e-83
ref|XP_003617703.1| Cytochrome P450 [Medicago truncatula] gi|355...   313   4e-83
ref|XP_003617706.1| Cytochrome P450 71D10 [Medicago truncatula] ...   308   1e-81

>gb|ACM92061.1| tabersonine 16-hydroxylase CYP71D12 [Catharanthus roseus]
            gi|325989357|gb|ADZ48683.1| tabersonine 16-hydroxylase
            [synthetic construct]
          Length = 506

 Score =  315 bits (807), Expect = 1e-83
 Identities = 156/309 (50%), Positives = 217/309 (70%), Gaps = 1/309 (0%)
 Frame = -1

Query: 924  REEEVMNMIKSISSQKGSAVNISRMMYSLTYTIIARCAFGKRSKHHDQYISLNEEATEIM 745
            REEEV+N IKSI S++G+ +N+S+ +  L Y I  R AFG+++K+ +++I L ++ T+ +
Sbjct: 155  REEEVLNFIKSIGSKEGTRINLSKEISLLIYGITTRAAFGEKNKNTEEFIRLLDQLTKAV 214

Query: 744  GGLSLVDSYPSLKILERITGIRKKMESNHEQVDEVLETIIGEHKRQRAEP-KIGXXXXXX 568
               ++ D +PSLK L+ I+  + K+E  H+Q D ++ETI+  HK +  +P          
Sbjct: 215  AEPNIADMFPSLKFLQLISTSKYKIEKIHKQFDVIVETILKGHKEKINKPLSQENGEKKE 274

Query: 567  XXXXXXLNFQESGELGSSLTDVNIKGAIYDIFSGGGQTSSRTLMWAMLEMMKNPTILKKA 388
                  LN Q   +  + L D NIK  I++IFS G +TSS T+ WAM EM+KNPT++KKA
Sbjct: 275  DLVDVLLNIQRRNDFEAPLGDKNIKAIIFNIFSAGTETSSTTVDWAMCEMIKNPTVMKKA 334

Query: 387  QDEVRKVIGAGGNVDETRLSELKYMRAVIKESLRFHPTGPLSIPRESSEQCEVGGYVIPA 208
            Q+EVRKV    GNVDET+L +LKY++AVIKE+LR HP  PL +PRE  EQC++ GY IP+
Sbjct: 335  QEEVRKVFNEEGNVDETKLHQLKYLQAVIKETLRLHPPVPLLLPRECREQCKIKGYTIPS 394

Query: 207  KTRVLVNIFAIGRDPKHWDDPEIFNPDRFLYSDIDFQGKDFSYIPFGAGRRVCPGITLAL 28
            K+RV+VN +AIGRDP +W +PE FNPDRFL S +DF+G  F Y+PFG GRR+CPGIT AL
Sbjct: 395  KSRVIVNAWAIGRDPNYWIEPEKFNPDRFLESKVDFKGNSFEYLPFGGGRRICPGITFAL 454

Query: 27   PNMELPLAQ 1
             N+ELPLAQ
Sbjct: 455  ANIELPLAQ 463


>sp|P98183.1|C71DC_CATRO RecName: Full=Tabersonine 16-hydroxylase; AltName: Full=Cytochrome
            P450 71D12 gi|5921278|emb|CAB56503.1| cytochrome P450
            [Catharanthus roseus]
          Length = 495

 Score =  315 bits (807), Expect = 1e-83
 Identities = 156/309 (50%), Positives = 217/309 (70%), Gaps = 1/309 (0%)
 Frame = -1

Query: 924  REEEVMNMIKSISSQKGSAVNISRMMYSLTYTIIARCAFGKRSKHHDQYISLNEEATEIM 745
            REEEV+N IKSI S++G+ +N+S+ +  L Y I  R AFG+++K+ +++I L ++ T+ +
Sbjct: 144  REEEVLNFIKSIGSKEGTRINLSKEISLLIYGITTRAAFGEKNKNTEEFIRLLDQLTKAV 203

Query: 744  GGLSLVDSYPSLKILERITGIRKKMESNHEQVDEVLETIIGEHKRQRAEP-KIGXXXXXX 568
               ++ D +PSLK L+ I+  + K+E  H+Q D ++ETI+  HK +  +P          
Sbjct: 204  AEPNIADMFPSLKFLQLISTSKYKIEKIHKQFDVIVETILKGHKEKINKPLSQENGEKKE 263

Query: 567  XXXXXXLNFQESGELGSSLTDVNIKGAIYDIFSGGGQTSSRTLMWAMLEMMKNPTILKKA 388
                  LN Q   +  + L D NIK  I++IFS G +TSS T+ WAM EM+KNPT++KKA
Sbjct: 264  DLVDVLLNIQRRNDFEAPLGDKNIKAIIFNIFSAGTETSSTTVDWAMCEMIKNPTVMKKA 323

Query: 387  QDEVRKVIGAGGNVDETRLSELKYMRAVIKESLRFHPTGPLSIPRESSEQCEVGGYVIPA 208
            Q+EVRKV    GNVDET+L +LKY++AVIKE+LR HP  PL +PRE  EQC++ GY IP+
Sbjct: 324  QEEVRKVFNEEGNVDETKLHQLKYLQAVIKETLRLHPPVPLLLPRECREQCKIKGYTIPS 383

Query: 207  KTRVLVNIFAIGRDPKHWDDPEIFNPDRFLYSDIDFQGKDFSYIPFGAGRRVCPGITLAL 28
            K+RV+VN +AIGRDP +W +PE FNPDRFL S +DF+G  F Y+PFG GRR+CPGIT AL
Sbjct: 384  KSRVIVNAWAIGRDPNYWIEPEKFNPDRFLESKVDFKGNSFEYLPFGGGRRICPGITFAL 443

Query: 27   PNMELPLAQ 1
             N+ELPLAQ
Sbjct: 444  ANIELPLAQ 452


>ref|XP_003634756.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 71D10-like [Vitis
            vinifera]
          Length = 554

 Score =  315 bits (806), Expect = 1e-83
 Identities = 150/307 (48%), Positives = 210/307 (68%)
 Frame = -1

Query: 924  REEEVMNMIKSISSQKGSAVNISRMMYSLTYTIIARCAFGKRSKHHDQYISLNEEATEIM 745
            RE EV  +I SIS   GS +N+S  ++SLTY I +R AFGK+ +  D ++S   EA E+ 
Sbjct: 200  REXEVSKLIWSISLNAGSPINLSEKIFSLTYGITSRSAFGKKFRGQDAFVSAILEAVELS 259

Query: 744  GGLSLVDSYPSLKILERITGIRKKMESNHEQVDEVLETIIGEHKRQRAEPKIGXXXXXXX 565
             G  + D YPSLK L  I+G++ K+E  H+++D +L  II +H++++   K G       
Sbjct: 260  AGFCVADMYPSLKWLHYISGMKPKLEKVHQKIDRILNNIIDDHRKRKTTTKAGQPETQED 319

Query: 564  XXXXXLNFQESGELGSSLTDVNIKGAIYDIFSGGGQTSSRTLMWAMLEMMKNPTILKKAQ 385
                 LN QE G+LG  LTD N+K  + DIFSGGG+TSS  ++WAM EM+K+P +++KAQ
Sbjct: 320  LVDVLLNLQEHGDLGIPLTDGNVKAVLLDIFSGGGETSSTAVVWAMAEMLKSPIVMEKAQ 379

Query: 384  DEVRKVIGAGGNVDETRLSELKYMRAVIKESLRFHPTGPLSIPRESSEQCEVGGYVIPAK 205
             EVR+V     +++ET + ELKY+ +V+KE+LR HP+ PL +PRE  E+C + GY IP  
Sbjct: 380  AEVRRVFDGKRDINETGIHELKYLNSVVKETLRLHPSVPLLLPRECRERCVINGYEIPEN 439

Query: 204  TRVLVNIFAIGRDPKHWDDPEIFNPDRFLYSDIDFQGKDFSYIPFGAGRRVCPGITLALP 25
            T+V++N +AI +DP HW +P  F P+RFL S IDF+G DF YIPFGAGRR+CPGI  A+P
Sbjct: 440  TKVIINAWAIAQDPDHWFEPNKFFPERFLDSSIDFKGTDFKYIPFGAGRRMCPGILFAIP 499

Query: 24   NMELPLA 4
            N+ELPLA
Sbjct: 500  NVELPLA 506


>ref|XP_003617703.1| Cytochrome P450 [Medicago truncatula] gi|355519038|gb|AET00662.1|
            Cytochrome P450 [Medicago truncatula]
          Length = 746

 Score =  313 bits (802), Expect = 4e-83
 Identities = 153/307 (49%), Positives = 210/307 (68%)
 Frame = -1

Query: 924  REEEVMNMIKSISSQKGSAVNISRMMYSLTYTIIARCAFGKRSKHHDQYISLNEEATEIM 745
            RE+EV N+  SI++ +GS VN++  ++S+TY I  R AFGKRSKH   + S  EE   ++
Sbjct: 400  REDEVSNLATSITASEGSIVNLTHKIFSMTYGITTRAAFGKRSKHQQSFKSAVEEIVSLL 459

Query: 744  GGLSLVDSYPSLKILERITGIRKKMESNHEQVDEVLETIIGEHKRQRAEPKIGXXXXXXX 565
            GG+ +VD YPS+K+L+ ++  + KME  H+++D +L+ II +HK    E           
Sbjct: 460  GGICIVDLYPSIKLLQCVSRAKAKMEKLHKEIDMILQDIIDDHKSIHKEDSNEEYLVDVL 519

Query: 564  XXXXXLNFQESGELGSSLTDVNIKGAIYDIFSGGGQTSSRTLMWAMLEMMKNPTILKKAQ 385
                  N+         LTD NIK  I DIF  G +TSS T++WA+ EM+KNP ++++AQ
Sbjct: 520  LKIQQENYHSQ----HPLTDDNIKSIIQDIFDAGTETSSTTVLWAISEMVKNPKVMEEAQ 575

Query: 384  DEVRKVIGAGGNVDETRLSELKYMRAVIKESLRFHPTGPLSIPRESSEQCEVGGYVIPAK 205
             EVR+V    G VDET L +L Y+++VIKE++R HPT PL +PRES E+C++ GY IPAK
Sbjct: 576  AEVRRVFDRKGFVDETELHQLIYLKSVIKETMRLHPTVPLLLPRESRERCQINGYEIPAK 635

Query: 204  TRVLVNIFAIGRDPKHWDDPEIFNPDRFLYSDIDFQGKDFSYIPFGAGRRVCPGITLALP 25
            TRV+VN +AIGRDP++W D E F P+RF+ S IDF+G DF YIPFGAGRR+CPGI  ALP
Sbjct: 636  TRVMVNAWAIGRDPRYWVDAESFKPERFVNSPIDFKGTDFEYIPFGAGRRMCPGIAFALP 695

Query: 24   NMELPLA 4
            N+ELPLA
Sbjct: 696  NVELPLA 702



 Score = 66.2 bits (160), Expect = 1e-08
 Identities = 32/88 (36%), Positives = 56/88 (63%)
 Frame = -1

Query: 915 EVMNMIKSISSQKGSAVNISRMMYSLTYTIIARCAFGKRSKHHDQYISLNEEATEIMGGL 736
           E+++  +  S  +GS VN+S  ++S+TY I AR  FGKR++H D +I   E+   ++G  
Sbjct: 142 ELLSAKRIQSFSEGSVVNLSEKIFSMTYGITARAVFGKRNRHQDVFIPALEKVVVLLGRF 201

Query: 735 SLVDSYPSLKILERITGIRKKMESNHEQ 652
            + D YPS+K+L+ +T  R+K ++  E+
Sbjct: 202 EIADLYPSIKLLQWMT--REKTKNRFEE 227


>ref|XP_003617706.1| Cytochrome P450 71D10 [Medicago truncatula]
            gi|355519041|gb|AET00665.1| Cytochrome P450 71D10
            [Medicago truncatula]
          Length = 502

 Score =  308 bits (789), Expect = 1e-81
 Identities = 148/308 (48%), Positives = 210/308 (68%)
 Frame = -1

Query: 924  REEEVMNMIKSISSQKGSAVNISRMMYSLTYTIIARCAFGKRSKHHDQYISLNEEATEIM 745
            REEEV +++KSIS+ +GS VN+S+ ++S+TY I+AR AFGK+++H   + S  EEA  ++
Sbjct: 156  REEEVSDLVKSISANEGSIVNLSKSIFSMTYGIVARSAFGKKNRHQQLFKSTIEEALGLL 215

Query: 744  GGLSLVDSYPSLKILERITGIRKKMESNHEQVDEVLETIIGEHKRQRAEPKIGXXXXXXX 565
            G   + D YPS+KIL++++ ++ ++E    ++D +L+ II +H+   ++           
Sbjct: 216  GEFCIADLYPSIKILQKVSRVKTRVERLQGEIDRILQDIINDHRNNHSKTSKDEDLVDVL 275

Query: 564  XXXXXLNFQESGELGSSLTDVNIKGAIYDIFSGGGQTSSRTLMWAMLEMMKNPTILKKAQ 385
                  N          LTD NIK  I D+F  G +TSS  ++WAM EM+KNP ++++AQ
Sbjct: 276  LKVQHENVHSQ----QPLTDENIKSVIQDLFIAGSETSSGIVLWAMSEMIKNPIVMEEAQ 331

Query: 384  DEVRKVIGAGGNVDETRLSELKYMRAVIKESLRFHPTGPLSIPRESSEQCEVGGYVIPAK 205
             EVR+V    G VDET L +L Y++ VIKE+ R HPT PL +PRES E+CE+ GY IPAK
Sbjct: 332  VEVRRVFDKKGYVDETELQQLTYLKCVIKETFRLHPTVPLLVPRESRERCEINGYEIPAK 391

Query: 204  TRVLVNIFAIGRDPKHWDDPEIFNPDRFLYSDIDFQGKDFSYIPFGAGRRVCPGITLALP 25
            TRV VN++AIGRDPK+W + E F P+RF+ S IDF+G DF  IPFGAGRR+CPGI  ALP
Sbjct: 392  TRVAVNVWAIGRDPKYWVEAESFKPERFVNSSIDFKGTDFELIPFGAGRRMCPGIAFALP 451

Query: 24   NMELPLAQ 1
            N+ELPLA+
Sbjct: 452  NVELPLAK 459