BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cephaelis21_contig00004211
         (1848 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADZ48682.1| cytochrome P450 [Catharanthus roseus]                  474   e-131
dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]       426   e-116
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]                   424   e-116
emb|CAA04117.1| cytochrome P450 [Helianthus tuberosus]                421   e-115
ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [V...   420   e-115

>gb|ADZ48682.1| cytochrome P450 [Catharanthus roseus]
          Length = 503

 Score =  474 bits (1220), Expect = e-131
 Identities = 242/453 (53%), Positives = 311/453 (68%), Gaps = 5/453 (1%)
 Frame = +3

Query: 231  GHLYLLKTAPHLTFQKLFIKYGPLISLRFGVCPILLVSSPSLAEECLTKTNDIIFANRPE 410
            GHL+LLKT  H T Q L  KYGP++ LRFG+ P L+VSSPS+ EE  TK ND+ FANRP+
Sbjct: 43   GHLHLLKTPLHRTLQFLAEKYGPIMLLRFGIRPTLIVSSPSIVEEIFTK-NDLAFANRPK 101

Query: 411  SFSTKYLCYNNTTLMVSPYGDHWRNLRRVTTINXXXXXXXXXXXXIWTEEIRFMIHKLLS 590
            S + K L YN TTL  SPYG+HWRNLRRV T++            IWTEE+R    +L +
Sbjct: 102  SIALKLLQYNYTTLGFSPYGNHWRNLRRVATVHIFSSLNIFHSSNIWTEEVRLSAKQLFA 161

Query: 591  GTSED--KNWKVIDMNSLFRELVFNVIMNMVAGKRWPSNQPTDLFTPHSMTNMCDYIPIL 764
             +++   + W  +++  LF ELV+NV+M M AGKRW S         +++ +  D+IPIL
Sbjct: 162  NSNKQNPEKWTELNLTDLFHELVYNVMMRMAAGKRW-SKSADSFKVVNTLIDTYDFIPIL 220

Query: 765  RWIGYGGL--EKSVISLNERRDKFAQDLIDETRHKKXXXXXXXXXXXXIIQALLSLQNAE 938
            RWIG+GG   EK + SL + RDKF QDLIDE R  +            ++Q+LL+LQ A+
Sbjct: 221  RWIGFGGYKYEKEMRSLQDERDKFLQDLIDEGRINRNESNSIDEGKKTVVQSLLALQEAD 280

Query: 939  PEYYTDAIVKGIILTMFVAGTHTSARTMERAMSNMLNHPEVLQKAKNELDNNLTQPGYLI 1118
            P+YYTD IVKG+I  MF AGT  SA  M   MS +LNHPEVL+KAK E+ N ++  G LI
Sbjct: 281  PQYYTDEIVKGMIQIMFTAGTDISAVAMLWTMSLLLNHPEVLEKAKTEIKNQISPAGKLI 340

Query: 1119 EDTDLSKLPYLRCIINETLRTFPPSPILAPHYSSEDCTIGGYRVSKGTTLLVNAWAIHRD 1298
            +D DL  LPYLR IINE+LR  PP+P+L PH++SEDCTI GY V K TT+L+N WAIHRD
Sbjct: 341  DDADLPNLPYLRSIINESLRLCPPAPLLLPHFTSEDCTINGYNVPKETTVLINTWAIHRD 400

Query: 1299 PNFWEEPDKFIPERFEEIDEGVCNE-GSKFIPFGKGRRACPGAALAMRVLGLSLGTLIQC 1475
            PN WEE  KF PERFE I+ G  ++ G KF+ FGKG+R+CPG+ LA+R + L++GTLIQ 
Sbjct: 401  PNVWEEATKFKPERFEGIEVGNSDQLGFKFVTFGKGKRSCPGSFLAVRFITLTIGTLIQS 460

Query: 1476 FDWKRVGPEMVDLEEKLDATMGKAKALEAMYKP 1574
            FDWKR+GPE+VD++EK   TM + K L A YKP
Sbjct: 461  FDWKRLGPELVDMQEKPGLTMHRDKPLVAFYKP 493


>dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 503

 Score =  426 bits (1094), Expect = e-116
 Identities = 226/470 (48%), Positives = 300/470 (63%), Gaps = 13/470 (2%)
 Frame = +3

Query: 231  GHLYLLKTAPHLTFQKLFIKYGPLISLRFGVCPILLVSSPSLAEECLTKTNDIIFANRPE 410
            GHL+L K   H T  KL  KYG ++ L+FG  P+L+VSSPS AE+CL K NDIIFANRP 
Sbjct: 39   GHLHLFKKPLHRTLAKLSDKYGHVLYLQFGSRPVLVVSSPSAAEDCLAK-NDIIFANRPH 97

Query: 411  SFSTKYLCYNNTTLMVSPYGDHWRNLRRVTTINXXXXXXXXXXXXIWTEEIRFMIHKLLS 590
                K L ++ TTL+ +PYG +WRNLR+++ I             I  +E++ M+ +L  
Sbjct: 98   LLMGKILGHDYTTLLWAPYGPNWRNLRKISAIELLSANRLQSYSDIRHDEVKSMVQRL-- 155

Query: 591  GTSEDKNWKVIDMNSLFRELVFNVIMNMVAGKRWPSNQPTDL-------------FTPHS 731
             + +   ++ ++M ++  EL  NV+M M+AGKR+   Q  +L                  
Sbjct: 156  -SGQGNEYRTVEMKTMIFELTLNVMMTMIAGKRYYGGQVEELEQAKRFQEIVQGTLAISG 214

Query: 732  MTNMCDYIPILRWIGYGGLEKSVISLNERRDKFAQDLIDETRHKKXXXXXXXXXXXXIIQ 911
             +N+ D++PIL WIG  G+EK V +L E+RDKF Q+LI+E R  +            +I 
Sbjct: 215  ASNIGDFLPILSWIGIKGIEKKVWNLKEKRDKFLQELIEEHRISRSGGMQTKEKNATLID 274

Query: 912  ALLSLQNAEPEYYTDAIVKGIILTMFVAGTHTSARTMERAMSNMLNHPEVLQKAKNELDN 1091
             LLSLQ  EPEYYTD ++ GII  +  AGT TSA TME AMS +LN+P+V++KA+ E+DN
Sbjct: 275  VLLSLQETEPEYYTDEMILGIIWVLLAAGTDTSAVTMEWAMSLLLNNPQVIKKAQAEIDN 334

Query: 1092 NLTQPGYLIEDTDLSKLPYLRCIINETLRTFPPSPILAPHYSSEDCTIGGYRVSKGTTLL 1271
            NL Q G LI ++D++KLPYL  II ETLR +P  P+L PH SSE+C +GGY+V  GT LL
Sbjct: 335  NLEQ-GRLINESDVNKLPYLHSIITETLRIYPAGPLLVPHESSEECIVGGYKVPSGTMLL 393

Query: 1272 VNAWAIHRDPNFWEEPDKFIPERFEEIDEGVCNEGSKFIPFGKGRRACPGAALAMRVLGL 1451
            VN WAI +DPN W EP KF PERF+   EG   +G K +PFG GRR CPG  LAMRV+ L
Sbjct: 394  VNVWAIQQDPNIWVEPTKFKPERFDGF-EGT-RDGFKLMPFGSGRRGCPGEGLAMRVVAL 451

Query: 1452 SLGTLIQCFDWKRVGPEMVDLEEKLDATMGKAKALEAMYKPRQSMINFIS 1601
            +LG LIQCFDW+RVG EMVD+ E    T+ K   LEA  +PR + +NFIS
Sbjct: 452  ALGALIQCFDWERVGEEMVDMSEGPGLTLPKVHPLEAKCRPRSTALNFIS 501


>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
          Length = 507

 Score =  424 bits (1090), Expect = e-116
 Identities = 223/474 (47%), Positives = 302/474 (63%), Gaps = 15/474 (3%)
 Frame = +3

Query: 231  GHLYLLKTAPHLTFQKLFIKYGPLISLRFGVCPILLVSSPSLAEECLTKTNDIIFANRPE 410
            GHL+L K   + T   +  +YGP++  +FG  P+L+VSSPS AEECLTK NDI FANRP 
Sbjct: 41   GHLHLFKKPLYRTLTNISNQYGPVLYFQFGSRPVLVVSSPSAAEECLTK-NDIGFANRPR 99

Query: 411  SFSTKYLCYNNTTLMVSPYGDHWRNLRRVTTINXXXXXXXXXXXXIWTEEIRFMIHKLLS 590
                K+   N TTL  + YG +WRNLRR++ +             I T+E++ M+ +L++
Sbjct: 100  LMIGKHSGNNYTTLAWASYGQNWRNLRRISALEMFSPNRIQMFSEIRTDEVKSMVRRLVA 159

Query: 591  GTSEDKNWKVIDMNSLFRELVFNVIMNMVAGKRWPSNQPTDL-------------FTPHS 731
            G    K    ++M S+F EL  N++M M+AGKR+      DL             F    
Sbjct: 160  GYEYHK----VEMKSVFFELTLNIMMRMIAGKRYYGEHVADLEQARKFKEIVEETFELSG 215

Query: 732  MTNMCDYIPILRWIGYGGLEKSVISLNERRDKFAQDLIDETRHKKXXXXXXXXXXXX--I 905
             +N+ D++P+LRWIGY G+EK +I L  +RD+F Q+LI E R  +              +
Sbjct: 216  ASNVGDFLPLLRWIGYKGIEKRLIRLKNKRDEFLQELIQERRKLRVNSKSFPSEEKKKTL 275

Query: 906  IQALLSLQNAEPEYYTDAIVKGIILTMFVAGTHTSARTMERAMSNMLNHPEVLQKAKNEL 1085
            I  LLSLQ  EPEYYTD +++G+I  +F AGT TSA TME AMS +LN+P++++KA++E+
Sbjct: 276  IDVLLSLQETEPEYYTDEMIRGLIWILFAAGTDTSAGTMEWAMSLLLNNPDLIKKAQSEI 335

Query: 1086 DNNLTQPGYLIEDTDLSKLPYLRCIINETLRTFPPSPILAPHYSSEDCTIGGYRVSKGTT 1265
            D  + +PG  I+++DL+KLPYL CIINETLR +P  P+L PH SSEDC IGGY +  GT 
Sbjct: 336  DLQI-EPGRPIDESDLNKLPYLHCIINETLRMYPAGPLLIPHESSEDCEIGGYNIPCGTM 394

Query: 1266 LLVNAWAIHRDPNFWEEPDKFIPERFEEIDEGVCNEGSKFIPFGKGRRACPGAALAMRVL 1445
            LLVN WAI  DPN W+EP KF PERFE    GV  +G K +PFG GRR CPG  LA RV+
Sbjct: 395  LLVNLWAIQNDPNLWKEPRKFKPERFEGYQGGV-RDGFKLMPFGSGRRGCPGEGLAXRVV 453

Query: 1446 GLSLGTLIQCFDWKRVGPEMVDLEEKLDATMGKAKALEAMYKPRQSMINFISDL 1607
            GL+L +L+QCFDW RVG EMV++ E    T+ K   LEA  +PR +M+NF+S +
Sbjct: 454  GLTLXSLLQCFDWHRVGEEMVEMSEGTGLTLPKLHPLEAHCRPRSTMLNFLSQV 507


>emb|CAA04117.1| cytochrome P450 [Helianthus tuberosus]
          Length = 520

 Score =  421 bits (1082), Expect = e-115
 Identities = 223/473 (47%), Positives = 298/473 (63%), Gaps = 14/473 (2%)
 Frame = +3

Query: 231  GHLYLLKTAPHLTFQKLFIKYGPLISLRFGVCPILLVSSPSLAEECLTKTNDIIFANRPE 410
            GHLYLLK   + T  KL  K+GP++ L+ G   +L+VSSPS AEEC TK NDI+FANRP+
Sbjct: 56   GHLYLLKPPLYRTLAKLSAKHGPILRLQLGFRRVLIVSSPSAAEECFTK-NDIVFANRPK 114

Query: 411  SFSTKYLCYNNTTLMVSPYGDHWRNLRRVTTINXXXXXXXXXXXXIWTEEIRFMIHKLLS 590
                K +  N T+L  SPYGD+WRNLRR+ +I             I  EE R +I KLLS
Sbjct: 115  MLFGKIIGVNYTSLAWSPYGDNWRNLRRIASIEILSIHRLNEFHDIRVEETRLLIQKLLS 174

Query: 591  GTSEDKNWKVIDMNSLFRELVFNVIMNMVAGKRWPSNQPTDL--------------FTPH 728
              +   +   + M   F EL  NV+M M++GKR+      +L              F   
Sbjct: 175  ACNSGSSQ--VTMKFSFYELTLNVMMRMISGKRYFGGDNPELEEEGKRFRDMLDETFVLA 232

Query: 729  SMTNMCDYIPILRWIGYGGLEKSVISLNERRDKFAQDLIDETRHKKXXXXXXXXXXXXII 908
              +N+ DY+P+L W+G  GLEK +I L E+RD F Q LID+ R  K            +I
Sbjct: 233  GASNVGDYLPVLSWLGVKGLEKKLIKLQEKRDVFFQGLIDQLRKSKGTEDVNKKKT--MI 290

Query: 909  QALLSLQNAEPEYYTDAIVKGIILTMFVAGTHTSARTMERAMSNMLNHPEVLQKAKNELD 1088
            + LLSLQ  EPEYYTDA+++  +L +  AG+ TSA TME  MS +LNHP+VL+KA+NE+D
Sbjct: 291  ELLLSLQETEPEYYTDAMIRSFVLVLLAAGSDTSAGTMEWVMSLLLNHPQVLKKAQNEID 350

Query: 1089 NNLTQPGYLIEDTDLSKLPYLRCIINETLRTFPPSPILAPHYSSEDCTIGGYRVSKGTTL 1268
            + + +   L++++D+  LPYLRCIINETLR +P  P+L PH +S DC +GGY V +GT L
Sbjct: 351  SVIGK-NCLVDESDIPNLPYLRCIINETLRLYPAGPLLVPHEASSDCVVGGYNVPRGTIL 409

Query: 1269 LVNAWAIHRDPNFWEEPDKFIPERFEEIDEGVCNEGSKFIPFGKGRRACPGAALAMRVLG 1448
            +VN WAIH DP  W+EP+ F PERFE + EG   +G K +PFG GRR+CPG  LA+R+LG
Sbjct: 410  IVNQWAIHHDPKVWDEPETFKPERFEGL-EGT-RDGFKLLPFGSGRRSCPGEGLAVRMLG 467

Query: 1449 LSLGTLIQCFDWKRVGPEMVDLEEKLDATMGKAKALEAMYKPRQSMINFISDL 1607
            ++LG++IQCFDW+R   E+VD+ E    TM KA  L A  KPR  M N +S+L
Sbjct: 468  MTLGSIIQCFDWERTSEELVDMTEGPGLTMPKAIPLVAKCKPRVEMTNLLSEL 520


>ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 503

 Score =  420 bits (1079), Expect = e-115
 Identities = 227/495 (45%), Positives = 310/495 (62%), Gaps = 13/495 (2%)
 Frame = +3

Query: 162  LTYYLQQQKKRXXXXXXXXXXXXGHLYLLKTAPHLTFQKLFIKYGPLISLRFGVCPILLV 341
            LT +  Q  K+            GHL+L K   H TF K+  +YGP++ +RFG  P+++V
Sbjct: 17   LTKHFFQSNKKLPPSPPLSLPIIGHLHLFKKPLHRTFAKISNQYGPILFIRFGSRPVIIV 76

Query: 342  SSPSLAEECLTKTNDIIFANRPESFSTKYLCYNNTTLMVSPYGDHWRNLRRVTTINXXXX 521
            SSPS AEEC TK NDI+FANRP   + K+L YN TTL  +PYG HWRNLRR+ ++     
Sbjct: 77   SSPSAAEECFTK-NDIVFANRPRLLAGKHLGYNYTTLTWAPYGQHWRNLRRIASLEILSS 135

Query: 522  XXXXXXXXIWTEEIRFMIHKLLSGTSEDKNWKVIDMNSLFRELVFNVIMNMVAGKRWPSN 701
                    I  +E+R ++ +L   +SE + +  +DM S+F EL  N +M M++GKR+  +
Sbjct: 136  NRLQMFYDIRIDEVRALLCQLFRASSEGQ-FSAVDMKSMFFELTLNNMMRMISGKRYYGD 194

Query: 702  QPTDL-------------FTPHSMTNMCDYIPILRWIGYGGLEKSVISLNERRDKFAQDL 842
              T+L             F     TN+ D++P  +WIG  G+EK ++ L  +RD F Q+L
Sbjct: 195  NVTELEETRKFREIVAETFELSGATNIVDFVPFSKWIGLNGIEKKLVILQGKRDGFMQNL 254

Query: 843  IDETRHKKXXXXXXXXXXXXIIQALLSLQNAEPEYYTDAIVKGIILTMFVAGTHTSARTM 1022
            I+E R  +            ++  LLSLQ  EPE YTD I++G++  M  AGT TSA TM
Sbjct: 255  IEEHRRMRSPCEGRSKT---MLDVLLSLQETEPECYTDDIIRGMMQVMLSAGTDTSAGTM 311

Query: 1023 ERAMSNMLNHPEVLQKAKNELDNNLTQPGYLIEDTDLSKLPYLRCIINETLRTFPPSPIL 1202
            E AMS +LN+PE L+KA+ E+D++L +   LI++ D+++LPYLR II ETLR +P +P+L
Sbjct: 312  EWAMSLLLNNPEALEKAQAEIDSHLGK-SRLIDELDIAELPYLRGIIKETLRMYPAAPLL 370

Query: 1203 APHYSSEDCTIGGYRVSKGTTLLVNAWAIHRDPNFWEEPDKFIPERFEEIDEGVCNEGSK 1382
             PH SSE+CT+GG+RV  GT LLVN WAI  DP  W EP KF PERF+   EG  N G  
Sbjct: 371  VPHESSEECTVGGFRVPSGTMLLVNMWAIQNDPMLWAEPSKFKPERFQG-PEGQRN-GFM 428

Query: 1383 FIPFGKGRRACPGAALAMRVLGLSLGTLIQCFDWKRVGPEMVDLEEKLDATMGKAKALEA 1562
            F PFG GRR CPG  LAMRV+GL+LG+LIQ F+W+RV  EMVD+ E    TM KA++L A
Sbjct: 429  FSPFGAGRRGCPGEGLAMRVVGLALGSLIQFFEWERVDEEMVDMSEGTGLTMPKAQSLVA 488

Query: 1563 MYKPRQSMINFISDL 1607
              +PR SM++ +S L
Sbjct: 489  KCRPRPSMVSLLSQL 503