BLASTX nr result
ID: Cephaelis21_contig00004062
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cephaelis21_contig00004062
(1718 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ADN92997.2| 12-oxophytodienoate reductase OPR3 [Ipomoea nil] 646 0.0
ref|NP_001233873.1| 12-oxophytodienoate reductase 3 [Solanum lyc... 640 0.0
pdb|2HS6|A Chain A, Crystal Structure Of The E291k Mutant Of 12-... 640 0.0
gb|AER51971.1| 12-oxophytodienoate reductase 3 [Solanum tuberosum] 640 0.0
pdb|2HS8|A Chain A, Crystal Structure Of The Y364f Mutant Of 12-... 639 0.0
>gb|ADN92997.2| 12-oxophytodienoate reductase OPR3 [Ipomoea nil]
Length = 400
Score = 646 bits (1667), Expect = 0.0
Identities = 312/389 (80%), Positives = 343/389 (88%)
Frame = +3
Query: 282 SPSLFSPFKMGKFNLSHRVVMAPMTRCRAINSIPQPALAEYYAQRATNGGFLICEGTMIS 461
+PSLFSP+KMG+FNLSHRVV+AP TRCRAIN IPQ ALAEYY+QRAT GGFLI EGTMI
Sbjct: 12 TPSLFSPYKMGRFNLSHRVVLAPCTRCRAINGIPQAALAEYYSQRATQGGFLITEGTMID 71
Query: 462 PTAAGFPHVPGIFTKEQVKAWKQVVDAVHAKGAVFFCQLWHVGRASHQVFQPGGAAPISS 641
PT+AGFPHVPGIFTKEQV+AWK+VVDAVHAKGA+ FCQLWHVGRASHQV+QPGG+APISS
Sbjct: 72 PTSAGFPHVPGIFTKEQVEAWKKVVDAVHAKGAIIFCQLWHVGRASHQVYQPGGSAPISS 131
Query: 642 TDKPISKRWRILMPDASKGIYPKPYPLTAHEIADVVEDYRQAALNAIEAGFDGIEIHGAH 821
TDKPISKRWRILMPD S GIYPKP + +IA+VVE YR AA+NAIEAGFDG+EIHGAH
Sbjct: 132 TDKPISKRWRILMPDGSHGIYPKPRAVGVDDIANVVEYYRIAAINAIEAGFDGVEIHGAH 191
Query: 822 GYLIDQFLKDGINDRKDEYGGPLANRCKFIMXXXXXXXXXIGADRVGVRISPAIDHLDAM 1001
GYLIDQFLKDGIN+RKDEYGG LANRCKFIM IGADR+GVRISPAIDHLDAM
Sbjct: 192 GYLIDQFLKDGINNRKDEYGGSLANRCKFIMQVVQAVATAIGADRLGVRISPAIDHLDAM 251
Query: 1002 ESDPLSLGLAVVERLNKLQQDLGSKLTYLHVTQPRYTAYGQTEAGRHGSEEEEARLMRTW 1181
+S PLSLGLAVVERLNKLQ D+ +KL YLHVTQPRY AYGQTE+G+ GSEEEEARLM T
Sbjct: 252 DSSPLSLGLAVVERLNKLQVDIRTKLAYLHVTQPRYVAYGQTESGKQGSEEEEARLMLTL 311
Query: 1182 RDAYQGTFICSGGYTRDLGIEAVAQGDADLVSYGRLFISNPDLPLRLRLNSPLTRYNRAT 1361
R+ YQGTFICSGGYTR LGIEA+AQGDADLVSYGRLFI+NPDL LR +L++PL +YNR T
Sbjct: 312 RNTYQGTFICSGGYTRQLGIEAIAQGDADLVSYGRLFIANPDLVLRFKLDAPLNKYNRKT 371
Query: 1362 FYTHDPVVGYTDYPFLGKNHGSYRPISRM 1448
FYT DPVVGYTDYPFL K GS RP SR+
Sbjct: 372 FYTQDPVVGYTDYPFLNKERGSNRPFSRL 400
>ref|NP_001233873.1| 12-oxophytodienoate reductase 3 [Solanum lycopersicum]
gi|62900706|sp|Q9FEW9.1|OPR3_SOLLC RecName:
Full=12-oxophytodienoate reductase 3; AltName:
Full=12-oxophytodienoate-10,11-reductase 3;
Short=OPDA-reductase 3; AltName: Full=LeOPR3
gi|12056507|emb|CAC21424.1| 12-oxophytodienoate reductase
3 [Solanum lycopersicum]
Length = 396
Score = 640 bits (1652), Expect = 0.0
Identities = 311/390 (79%), Positives = 344/390 (88%)
Frame = +3
Query: 279 GSPSLFSPFKMGKFNLSHRVVMAPMTRCRAINSIPQPALAEYYAQRATNGGFLICEGTMI 458
G+ LFSP+KMGKFNLSHRVV+APMTRCRA+N+IPQ AL EYY QRAT GGFLI EGTMI
Sbjct: 8 GNNPLFSPYKMGKFNLSHRVVLAPMTRCRALNNIPQAALGEYYEQRATAGGFLITEGTMI 67
Query: 459 SPTAAGFPHVPGIFTKEQVKAWKQVVDAVHAKGAVFFCQLWHVGRASHQVFQPGGAAPIS 638
SPT+AGFPHVPGIFTKEQV+ WK++VD VHAKGAV FCQLWHVGRASH+V+QP GAAPIS
Sbjct: 68 SPTSAGFPHVPGIFTKEQVREWKKIVDVVHAKGAVIFCQLWHVGRASHEVYQPAGAAPIS 127
Query: 639 STDKPISKRWRILMPDASKGIYPKPYPLTAHEIADVVEDYRQAALNAIEAGFDGIEIHGA 818
ST+KPIS RWRILMPD + GIYPKP + +EI+ VVEDYR++ALNAIEAGFDGIEIHGA
Sbjct: 128 STEKPISNRWRILMPDGTHGIYPKPRAIGTYEISQVVEDYRRSALNAIEAGFDGIEIHGA 187
Query: 819 HGYLIDQFLKDGINDRKDEYGGPLANRCKFIMXXXXXXXXXIGADRVGVRISPAIDHLDA 998
HGYLIDQFLKDGINDR DEYGG LANRCKFI IGADRVGVR+SPAIDHLDA
Sbjct: 188 HGYLIDQFLKDGINDRTDEYGGSLANRCKFITQVVQAVVSAIGADRVGVRVSPAIDHLDA 247
Query: 999 MESDPLSLGLAVVERLNKLQQDLGSKLTYLHVTQPRYTAYGQTEAGRHGSEEEEARLMRT 1178
M+S+PLSLGLAVVERLNK+Q GSKL YLHVTQPRY AYGQTEAGR GSEEEEARLMRT
Sbjct: 248 MDSNPLSLGLAVVERLNKIQLHSGSKLAYLHVTQPRYVAYGQTEAGRLGSEEEEARLMRT 307
Query: 1179 WRDAYQGTFICSGGYTRDLGIEAVAQGDADLVSYGRLFISNPDLPLRLRLNSPLTRYNRA 1358
R+AYQGTFICSGGYTR+LGIEAVAQGDADLVSYGRLFISNPDL +R++LN+PL +YNR
Sbjct: 308 LRNAYQGTFICSGGYTRELGIEAVAQGDADLVSYGRLFISNPDLVMRIKLNAPLNKYNRK 367
Query: 1359 TFYTHDPVVGYTDYPFLGKNHGSYRPISRM 1448
TFYT DPVVGYTDYPFL + +GS P+SR+
Sbjct: 368 TFYTQDPVVGYTDYPFL-QGNGSNGPLSRL 396
>pdb|2HS6|A Chain A, Crystal Structure Of The E291k Mutant Of 12-
Oxophytodienoate Reductase 3 (Opr3) From Tomato
gi|116667815|pdb|2HS6|B Chain B, Crystal Structure Of The
E291k Mutant Of 12- Oxophytodienoate Reductase 3 (Opr3)
From Tomato gi|116667818|pdb|2HSA|B Chain B, Crystal
Structure Of 12-Oxophytodienoate Reductase 3 (Opr3) From
Tomato gi|116667819|pdb|2HSA|A Chain A, Crystal Structure
Of 12-Oxophytodienoate Reductase 3 (Opr3) From Tomato
gi|256599757|pdb|3HGS|A Chain A, Crystal Structure Of
Tomato Opr3 In Complex With Phb gi|256599758|pdb|3HGS|B
Chain B, Crystal Structure Of Tomato Opr3 In Complex With
Phb
Length = 402
Score = 640 bits (1652), Expect = 0.0
Identities = 311/390 (79%), Positives = 344/390 (88%)
Frame = +3
Query: 279 GSPSLFSPFKMGKFNLSHRVVMAPMTRCRAINSIPQPALAEYYAQRATNGGFLICEGTMI 458
G+ LFSP+KMGKFNLSHRVV+APMTRCRA+N+IPQ AL EYY QRAT GGFLI EGTMI
Sbjct: 14 GNNPLFSPYKMGKFNLSHRVVLAPMTRCRALNNIPQAALGEYYEQRATAGGFLITEGTMI 73
Query: 459 SPTAAGFPHVPGIFTKEQVKAWKQVVDAVHAKGAVFFCQLWHVGRASHQVFQPGGAAPIS 638
SPT+AGFPHVPGIFTKEQV+ WK++VD VHAKGAV FCQLWHVGRASH+V+QP GAAPIS
Sbjct: 74 SPTSAGFPHVPGIFTKEQVREWKKIVDVVHAKGAVIFCQLWHVGRASHEVYQPAGAAPIS 133
Query: 639 STDKPISKRWRILMPDASKGIYPKPYPLTAHEIADVVEDYRQAALNAIEAGFDGIEIHGA 818
ST+KPIS RWRILMPD + GIYPKP + +EI+ VVEDYR++ALNAIEAGFDGIEIHGA
Sbjct: 134 STEKPISNRWRILMPDGTHGIYPKPRAIGTYEISQVVEDYRRSALNAIEAGFDGIEIHGA 193
Query: 819 HGYLIDQFLKDGINDRKDEYGGPLANRCKFIMXXXXXXXXXIGADRVGVRISPAIDHLDA 998
HGYLIDQFLKDGINDR DEYGG LANRCKFI IGADRVGVR+SPAIDHLDA
Sbjct: 194 HGYLIDQFLKDGINDRTDEYGGSLANRCKFITQVVQAVVSAIGADRVGVRVSPAIDHLDA 253
Query: 999 MESDPLSLGLAVVERLNKLQQDLGSKLTYLHVTQPRYTAYGQTEAGRHGSEEEEARLMRT 1178
M+S+PLSLGLAVVERLNK+Q GSKL YLHVTQPRY AYGQTEAGR GSEEEEARLMRT
Sbjct: 254 MDSNPLSLGLAVVERLNKIQLHSGSKLAYLHVTQPRYVAYGQTEAGRLGSEEEEARLMRT 313
Query: 1179 WRDAYQGTFICSGGYTRDLGIEAVAQGDADLVSYGRLFISNPDLPLRLRLNSPLTRYNRA 1358
R+AYQGTFICSGGYTR+LGIEAVAQGDADLVSYGRLFISNPDL +R++LN+PL +YNR
Sbjct: 314 LRNAYQGTFICSGGYTRELGIEAVAQGDADLVSYGRLFISNPDLVMRIKLNAPLNKYNRK 373
Query: 1359 TFYTHDPVVGYTDYPFLGKNHGSYRPISRM 1448
TFYT DPVVGYTDYPFL + +GS P+SR+
Sbjct: 374 TFYTQDPVVGYTDYPFL-QGNGSNGPLSRL 402
>gb|AER51971.1| 12-oxophytodienoate reductase 3 [Solanum tuberosum]
Length = 400
Score = 640 bits (1650), Expect = 0.0
Identities = 311/401 (77%), Positives = 347/401 (86%)
Frame = +3
Query: 246 MGMAAETKSCHGSPSLFSPFKMGKFNLSHRVVMAPMTRCRAINSIPQPALAEYYAQRATN 425
M + + G+ LFSP+KMGKFNLSHR+V+APMTRCRA+N+IPQ AL EYY QRAT
Sbjct: 1 MANTTSSSAQDGNNPLFSPYKMGKFNLSHRIVLAPMTRCRALNNIPQAALGEYYEQRATA 60
Query: 426 GGFLICEGTMISPTAAGFPHVPGIFTKEQVKAWKQVVDAVHAKGAVFFCQLWHVGRASHQ 605
GGFLI EGTMISPT+AGFPHVPGIFTKEQV+ WK++VD VHAKGAV FCQLWHVGRASH+
Sbjct: 61 GGFLITEGTMISPTSAGFPHVPGIFTKEQVEEWKKIVDVVHAKGAVIFCQLWHVGRASHE 120
Query: 606 VFQPGGAAPISSTDKPISKRWRILMPDASKGIYPKPYPLTAHEIADVVEDYRQAALNAIE 785
V+QP GAAPISST+ PISKRWRILMPD + GIYPKP + +EI+ VVEDYR++ALNAIE
Sbjct: 121 VYQPAGAAPISSTEMPISKRWRILMPDGTHGIYPKPRAIGTYEISQVVEDYRRSALNAIE 180
Query: 786 AGFDGIEIHGAHGYLIDQFLKDGINDRKDEYGGPLANRCKFIMXXXXXXXXXIGADRVGV 965
AGFDGIEIHGAHGYLIDQFLKDGINDR DEYGG LANRCKFI IGADRVGV
Sbjct: 181 AGFDGIEIHGAHGYLIDQFLKDGINDRTDEYGGSLANRCKFITQVVQAVVSAIGADRVGV 240
Query: 966 RISPAIDHLDAMESDPLSLGLAVVERLNKLQQDLGSKLTYLHVTQPRYTAYGQTEAGRHG 1145
R+SPAIDHLDAM+S+PLSLGLAVVERLNK+Q GSKL YLHVTQPRY AYGQTEAGR G
Sbjct: 241 RVSPAIDHLDAMDSNPLSLGLAVVERLNKIQLHSGSKLAYLHVTQPRYVAYGQTEAGRLG 300
Query: 1146 SEEEEARLMRTWRDAYQGTFICSGGYTRDLGIEAVAQGDADLVSYGRLFISNPDLPLRLR 1325
SEEEEARLMRT R+AYQGTFICSGGYTR+LGIEAVAQGDADLVSYGRLFISNPDL +R++
Sbjct: 301 SEEEEARLMRTLRNAYQGTFICSGGYTRELGIEAVAQGDADLVSYGRLFISNPDLVMRIK 360
Query: 1326 LNSPLTRYNRATFYTHDPVVGYTDYPFLGKNHGSYRPISRM 1448
LN+PL +YNR TFYT DPVVGYTDYPFL + +GS P+SR+
Sbjct: 361 LNAPLNKYNRKTFYTQDPVVGYTDYPFL-QGNGSNGPLSRL 400
>pdb|2HS8|A Chain A, Crystal Structure Of The Y364f Mutant Of 12-
Oxophytodienoate Reductase 3 From Tomato
gi|116667817|pdb|2HS8|B Chain B, Crystal Structure Of The
Y364f Mutant Of 12- Oxophytodienoate Reductase 3 From
Tomato
Length = 402
Score = 639 bits (1648), Expect = 0.0
Identities = 310/390 (79%), Positives = 344/390 (88%)
Frame = +3
Query: 279 GSPSLFSPFKMGKFNLSHRVVMAPMTRCRAINSIPQPALAEYYAQRATNGGFLICEGTMI 458
G+ LFSP+KMGKFNLSHRVV+APMTRCRA+N+IPQ AL EYY QRAT GGFLI EGTMI
Sbjct: 14 GNNPLFSPYKMGKFNLSHRVVLAPMTRCRALNNIPQAALGEYYEQRATAGGFLITEGTMI 73
Query: 459 SPTAAGFPHVPGIFTKEQVKAWKQVVDAVHAKGAVFFCQLWHVGRASHQVFQPGGAAPIS 638
SPT+AGFPHVPGIFTKEQV+ WK++VD VHAKGAV FCQLWHVGRASH+V+QP GAAPIS
Sbjct: 74 SPTSAGFPHVPGIFTKEQVREWKKIVDVVHAKGAVIFCQLWHVGRASHEVYQPAGAAPIS 133
Query: 639 STDKPISKRWRILMPDASKGIYPKPYPLTAHEIADVVEDYRQAALNAIEAGFDGIEIHGA 818
ST+KPIS RWRILMPD + GIYPKP + +EI+ VVEDYR++ALNAIEAGFDGIEIHGA
Sbjct: 134 STEKPISNRWRILMPDGTHGIYPKPRAIGTYEISQVVEDYRRSALNAIEAGFDGIEIHGA 193
Query: 819 HGYLIDQFLKDGINDRKDEYGGPLANRCKFIMXXXXXXXXXIGADRVGVRISPAIDHLDA 998
HGYLIDQFLKDGINDR DEYGG LANRCKFI IGADRVGVR+SPAIDHLDA
Sbjct: 194 HGYLIDQFLKDGINDRTDEYGGSLANRCKFITQVVQAVVSAIGADRVGVRVSPAIDHLDA 253
Query: 999 MESDPLSLGLAVVERLNKLQQDLGSKLTYLHVTQPRYTAYGQTEAGRHGSEEEEARLMRT 1178
M+S+PLSLGLAVVERLNK+Q GSKL YLHVTQPRY AYGQTEAGR GSEEEEARLMRT
Sbjct: 254 MDSNPLSLGLAVVERLNKIQLHSGSKLAYLHVTQPRYVAYGQTEAGRLGSEEEEARLMRT 313
Query: 1179 WRDAYQGTFICSGGYTRDLGIEAVAQGDADLVSYGRLFISNPDLPLRLRLNSPLTRYNRA 1358
R+AYQGTFICSGGYTR+LGIEAVAQGDADLVSYGRLFISNPDL +R++LN+PL ++NR
Sbjct: 314 LRNAYQGTFICSGGYTRELGIEAVAQGDADLVSYGRLFISNPDLVMRIKLNAPLNKFNRK 373
Query: 1359 TFYTHDPVVGYTDYPFLGKNHGSYRPISRM 1448
TFYT DPVVGYTDYPFL + +GS P+SR+
Sbjct: 374 TFYTQDPVVGYTDYPFL-QGNGSNGPLSRL 402