BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cephaelis21_contig00000772
         (2270 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAA17746.1| cytochrome P450, partial [Catharanthus roseus]         833   0.0  
gb|AAA17732.1| cytochrome P450 [Catharanthus roseus]                  830   0.0  
sp|Q05047.1|C72A1_CATRO RecName: Full=Secologanin synthase; Shor...   829   0.0  
gb|ADU19849.1| putative secologanin synthase [Camptotheca acumin...   699   0.0  
dbj|BAL45206.1| cytochrome P450 monooxygenase [Glycyrrhiza urale...   575   e-161

>gb|AAA17746.1| cytochrome P450, partial [Catharanthus roseus]
          Length = 516

 Score =  833 bits (2152), Expect = 0.0
 Identities = 403/505 (79%), Positives = 454/505 (89%)
 Frame = -2

Query: 2008 WRILSWAWFTPKSIEKRLRKQGFRGNPYRFLVGDIKDSTMMLQKAMAKPIPLCNDIVARI 1829
            WR+L WAWFTPK IEKRLR+QGFRGNPYRFLVGD+K+S  M Q+A++ P+   NDIV R+
Sbjct: 14   WRVLDWAWFTPKRIEKRLRQQGFRGNPYRFLVGDVKESGKMHQEALSNPMEFDNDIVPRL 73

Query: 1828 MPHVDHTIKTYGRNSFTWVGRIPRIHVMDPDLIREVLTHSTKYVKNFDIHDPLVKFLLTG 1649
            MPH++HTIKTYGRNSFTW+GRIPRIHVM+P+LI+EVLTHS+KY KNFD+H+PLVKFLLTG
Sbjct: 74   MPHINHTIKTYGRNSFTWMGRIPRIHVMEPELIKEVLTHSSKYQKNFDVHNPLVKFLLTG 133

Query: 1648 VGSFEGEKWSRHRRIISPAFTLEKLKKMLPAFAVCYDDLLNKWENTCTREGSIEVDIFPT 1469
            VGSFEG KWS+HRRIISPAFTLEKLK MLPAFA+CY D+L KWE    +EGS EVDIFPT
Sbjct: 134  VGSFEGAKWSKHRRIISPAFTLEKLKSMLPAFAICYHDMLTKWEKLAEKEGSHEVDIFPT 193

Query: 1468 FDVLTSDVISKVAFDSTYEESGRTFRLLKELMDLTMEAIREVYIPGWSYLPTKRNRRMKL 1289
            FDVLTSDVISKVAF STY+E G+ FRLLKELMDLT++ +R+VYIPGWSYLPTKRN+RMK 
Sbjct: 194  FDVLTSDVISKVAFGSTYDEGGKIFRLLKELMDLTIDCMRDVYIPGWSYLPTKRNKRMKE 253

Query: 1288 INHEMREFLRNIIDKRVKARKAGELPEEEDLLGVLLESNFQEIEKQGNDHKQNVGMSIDD 1109
            IN E+ + LR II+KR+KA KAGE P E+DLLGVLLESN QEI+KQGN  +++ GM+I+D
Sbjct: 254  INKEITDMLRFIINKRMKALKAGE-PGEDDLLGVLLESNIQEIQKQGN--RKDGGMTIND 310

Query: 1108 VIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERARAEVFQAFGNKKPDDFEKLNHLK 929
            VIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAR EV QAFG  KP +FE+LNHLK
Sbjct: 311  VIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREEVLQAFGKNKP-EFERLNHLK 369

Query: 928  CVSMILYEVLRLYPPVIDLTKIVHEDTKLGPYTIPGGTQVMMPSVMIHREKSIWGEDAME 749
             VSMILYEVLRLYPPVIDLTKI+HEDTKLGPYTIP GTQVM+P+VM+HREKSIWGEDAME
Sbjct: 370  YVSMILYEVLRLYPPVIDLTKIIHEDTKLGPYTIPAGTQVMLPTVMLHREKSIWGEDAME 429

Query: 748  FNPERFADGVANATKNGALAYLPFSWGPRVCLGQNFALLQAKLGLVMILQRFSFDVSPSY 569
            FNP RFADGVANATKN  + YLPFSWGPRVCLGQNFALLQAKLGL MILQRF FDV+PSY
Sbjct: 430  FNPMRFADGVANATKNN-VTYLPFSWGPRVCLGQNFALLQAKLGLAMILQRFKFDVAPSY 488

Query: 568  AHAPYTVLTLQPQFGSHVIYKKLIR 494
             HAP+T+LT+QPQFGSHVIYKKL R
Sbjct: 489  VHAPFTILTVQPQFGSHVIYKKLER 513


>gb|AAA17732.1| cytochrome P450 [Catharanthus roseus]
          Length = 524

 Score =  830 bits (2144), Expect = 0.0
 Identities = 402/503 (79%), Positives = 452/503 (89%)
 Frame = -2

Query: 2008 WRILSWAWFTPKSIEKRLRKQGFRGNPYRFLVGDIKDSTMMLQKAMAKPIPLCNDIVARI 1829
            WR+L WAWFTPK IEKRLR+QGFRGNPYRFLVGD+K+S  M Q+A++KP+   NDIV R+
Sbjct: 25   WRVLDWAWFTPKRIEKRLRQQGFRGNPYRFLVGDVKESGKMHQEALSKPMEFNNDIVPRL 84

Query: 1828 MPHVDHTIKTYGRNSFTWVGRIPRIHVMDPDLIREVLTHSTKYVKNFDIHDPLVKFLLTG 1649
            MPH++HTI TYG NSFTW+GRIPRIHVM+P+LI+EVLTHS+KY KNFD+H+PLVKFLLTG
Sbjct: 85   MPHINHTINTYGGNSFTWMGRIPRIHVMEPELIKEVLTHSSKYQKNFDVHNPLVKFLLTG 144

Query: 1648 VGSFEGEKWSRHRRIISPAFTLEKLKKMLPAFAVCYDDLLNKWENTCTREGSIEVDIFPT 1469
            VGSFEG KWS+HRRIISPAFTLEKLK MLPAFA+CY D+L KWE    ++GS E+DIFPT
Sbjct: 145  VGSFEGAKWSKHRRIISPAFTLEKLKSMLPAFAICYHDMLTKWEKIAEKQGSHEIDIFPT 204

Query: 1468 FDVLTSDVISKVAFDSTYEESGRTFRLLKELMDLTMEAIREVYIPGWSYLPTKRNRRMKL 1289
            FDVLTSDVISKVAF STYEE G+ FRLLKELMDLT++ +R+VYIPGWSYLPTKRN+RMK 
Sbjct: 205  FDVLTSDVISKVAFGSTYEEGGKIFRLLKELMDLTIDCMRDVYIPGWSYLPTKRNKRMKE 264

Query: 1288 INHEMREFLRNIIDKRVKARKAGELPEEEDLLGVLLESNFQEIEKQGNDHKQNVGMSIDD 1109
            IN E+ + LR II+KR+KA KAGE P E+DLLGVLLESN QEI+KQGN  K++ GMSI+D
Sbjct: 265  INKEITDMLRFIINKRMKALKAGE-PGEDDLLGVLLESNIQEIQKQGN--KKDGGMSIND 321

Query: 1108 VIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERARAEVFQAFGNKKPDDFEKLNHLK 929
            VIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAR EV QAFG  KP +FE+LNHLK
Sbjct: 322  VIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREEVLQAFGKNKP-EFERLNHLK 380

Query: 928  CVSMILYEVLRLYPPVIDLTKIVHEDTKLGPYTIPGGTQVMMPSVMIHREKSIWGEDAME 749
             VSMILYEVLRLYPPVIDLTKIVHEDTKLGPYTIP GTQVM+P+VM+HREKSIWGEDA E
Sbjct: 381  YVSMILYEVLRLYPPVIDLTKIVHEDTKLGPYTIPAGTQVMLPTVMLHREKSIWGEDATE 440

Query: 748  FNPERFADGVANATKNGALAYLPFSWGPRVCLGQNFALLQAKLGLVMILQRFSFDVSPSY 569
            FNP RFADGVANATKN  + YLPFSWGPRVCLGQNFALLQAKLGL MILQRF+FDV+PSY
Sbjct: 441  FNPMRFADGVANATKNN-VTYLPFSWGPRVCLGQNFALLQAKLGLAMILQRFTFDVAPSY 499

Query: 568  AHAPYTVLTLQPQFGSHVIYKKL 500
             HAP+T+LT+QPQFGSHVIYKKL
Sbjct: 500  VHAPFTILTVQPQFGSHVIYKKL 522


>sp|Q05047.1|C72A1_CATRO RecName: Full=Secologanin synthase; Short=SLS; AltName:
            Full=CYPLXXII; AltName: Full=Cytochrome P450 72A1
            gi|167484|gb|AAA33106.1| cytochrome P-450 protein
            [Catharanthus roseus] gi|445604|prf||1909351A cytochrome
            P450
          Length = 524

 Score =  829 bits (2141), Expect = 0.0
 Identities = 402/503 (79%), Positives = 451/503 (89%)
 Frame = -2

Query: 2008 WRILSWAWFTPKSIEKRLRKQGFRGNPYRFLVGDIKDSTMMLQKAMAKPIPLCNDIVARI 1829
            WR+L WAWFTPK IEKRLR+QGFRGNPYRFLVGD+K+S  M Q+A++KP+   NDIV R+
Sbjct: 25   WRVLDWAWFTPKRIEKRLRQQGFRGNPYRFLVGDVKESGKMHQEALSKPMEFNNDIVPRL 84

Query: 1828 MPHVDHTIKTYGRNSFTWVGRIPRIHVMDPDLIREVLTHSTKYVKNFDIHDPLVKFLLTG 1649
            MPH++HTI TYGRNSFTW+GRIPRIHVM+P+LI+EVLTHS+KY KNFD+H+PLVKFLLTG
Sbjct: 85   MPHINHTINTYGRNSFTWMGRIPRIHVMEPELIKEVLTHSSKYQKNFDVHNPLVKFLLTG 144

Query: 1648 VGSFEGEKWSRHRRIISPAFTLEKLKKMLPAFAVCYDDLLNKWENTCTREGSIEVDIFPT 1469
            VGSFEG KWS+HRRIISPAFTLEKLK MLPAFA+CY D+L KWE    ++GS EVDIFPT
Sbjct: 145  VGSFEGAKWSKHRRIISPAFTLEKLKSMLPAFAICYHDMLTKWEKIAEKQGSHEVDIFPT 204

Query: 1468 FDVLTSDVISKVAFDSTYEESGRTFRLLKELMDLTMEAIREVYIPGWSYLPTKRNRRMKL 1289
            FDVLTSDVISKVAF STYEE G+ FRLLKELMDLT++ +R+VYIPGWSYLPTKRN+RMK 
Sbjct: 205  FDVLTSDVISKVAFGSTYEEGGKIFRLLKELMDLTIDCMRDVYIPGWSYLPTKRNKRMKE 264

Query: 1288 INHEMREFLRNIIDKRVKARKAGELPEEEDLLGVLLESNFQEIEKQGNDHKQNVGMSIDD 1109
            IN E+ + LR II+KR+KA KAGE P E+DLLGVLLESN QEI+KQGN  K++ GMSI+D
Sbjct: 265  INKEITDMLRFIINKRMKALKAGE-PGEDDLLGVLLESNIQEIQKQGN--KKDGGMSIND 321

Query: 1108 VIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERARAEVFQAFGNKKPDDFEKLNHLK 929
            VIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAR EV QAFG  KP +FE+LNHLK
Sbjct: 322  VIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREEVLQAFGKNKP-EFERLNHLK 380

Query: 928  CVSMILYEVLRLYPPVIDLTKIVHEDTKLGPYTIPGGTQVMMPSVMIHREKSIWGEDAME 749
             VSMILYEVLRLYPPVIDLTKIVH+DTKLG YTIP GTQVM+P+VM+HREKSIWGEDAME
Sbjct: 381  YVSMILYEVLRLYPPVIDLTKIVHKDTKLGSYTIPAGTQVMLPTVMLHREKSIWGEDAME 440

Query: 748  FNPERFADGVANATKNGALAYLPFSWGPRVCLGQNFALLQAKLGLVMILQRFSFDVSPSY 569
            FNP RF DGVANATKN  + YLPFSWGPRVCLGQNFALLQAKLGL MILQRF FDV+PSY
Sbjct: 441  FNPMRFVDGVANATKNN-VTYLPFSWGPRVCLGQNFALLQAKLGLAMILQRFKFDVAPSY 499

Query: 568  AHAPYTVLTLQPQFGSHVIYKKL 500
             HAP+T+LT+QPQFGSHVIYKKL
Sbjct: 500  VHAPFTILTVQPQFGSHVIYKKL 522


>gb|ADU19849.1| putative secologanin synthase [Camptotheca acuminata]
          Length = 524

 Score =  699 bits (1804), Expect = 0.0
 Identities = 329/523 (62%), Positives = 424/523 (81%)
 Frame = -2

Query: 2068 VDILMRXXXXXXXXXXXXXSWRILSWAWFTPKSIEKRLRKQGFRGNPYRFLVGDIKDSTM 1889
            +D+L +             +W++L+WA+ TPK IEK LRKQGF+GN YR LVGD+K+S+M
Sbjct: 5    MDVLYKSIAASVAVVFLVYAWKMLNWAYLTPKRIEKCLRKQGFKGNSYRLLVGDLKESSM 64

Query: 1888 MLQKAMAKPIPLCNDIVARIMPHVDHTIKTYGRNSFTWVGRIPRIHVMDPDLIREVLTHS 1709
            ML++ M+KPI +  DIV R+MPHV  TI TYG+NSFTW+GR+PR+H+M+PDLI+++L + 
Sbjct: 65   MLKETMSKPINVSEDIVERVMPHVIKTIDTYGKNSFTWIGRMPRVHIMEPDLIKDILANH 124

Query: 1708 TKYVKNFDIHDPLVKFLLTGVGSFEGEKWSRHRRIISPAFTLEKLKKMLPAFAVCYDDLL 1529
              ++KN   ++PL KFLLTG+GS EG+KW++HRRIISP+F LEKLK MLPAF V YDDLL
Sbjct: 125  NDFMKNHHAYNPLTKFLLTGIGSLEGDKWAKHRRIISPSFHLEKLKTMLPAFYVSYDDLL 184

Query: 1528 NKWENTCTREGSIEVDIFPTFDVLTSDVISKVAFDSTYEESGRTFRLLKELMDLTMEAIR 1349
             KWE  C+ +GS+E+D+FPTFD LTSDVIS+VAF S+Y E GR F LLKELMDLT++ +R
Sbjct: 185  TKWEQQCSSKGSVEIDLFPTFDTLTSDVISRVAFGSSYGEGGRIFILLKELMDLTVDVMR 244

Query: 1348 EVYIPGWSYLPTKRNRRMKLINHEMREFLRNIIDKRVKARKAGELPEEEDLLGVLLESNF 1169
             VY+PG S+LPTKRN RM+ ++ E+++ L  II+ RVKA KAGE P  EDLLG LLESNF
Sbjct: 245  SVYVPGSSFLPTKRNNRMREVDGEIKDRLSGIINSRVKAMKAGE-PSGEDLLGTLLESNF 303

Query: 1168 QEIEKQGNDHKQNVGMSIDDVIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERARAE 989
            +EIE+ GN  K+N GMSI+DVI ECKLFYFAGQETTG+LLTWT +LLS+HPEWQERAR E
Sbjct: 304  KEIERLGN--KKNAGMSIEDVISECKLFYFAGQETTGILLTWTCVLLSRHPEWQERAREE 361

Query: 988  VFQAFGNKKPDDFEKLNHLKCVSMILYEVLRLYPPVIDLTKIVHEDTKLGPYTIPGGTQV 809
            +FQ FGN K  DF+++ +LK V MILYEVLRLYPPVI+LTK+ +E+ KLG  TIP G Q+
Sbjct: 362  IFQVFGNGKV-DFDRVQNLKIVPMILYEVLRLYPPVIELTKVTYEEQKLGNLTIPAGVQL 420

Query: 808  MMPSVMIHREKSIWGEDAMEFNPERFADGVANATKNGALAYLPFSWGPRVCLGQNFALLQ 629
            MMPS+++HR++ +WG D+ EFNP RFADG++ A K+    Y+PFSWGPR+C+GQNFALLQ
Sbjct: 421  MMPSILLHRDQEMWGADSKEFNPGRFADGISKAVKS-PFFYIPFSWGPRICVGQNFALLQ 479

Query: 628  AKLGLVMILQRFSFDVSPSYAHAPYTVLTLQPQFGSHVIYKKL 500
            AK+ L MILQRF+FD+SP+YAHAP+TVLTLQPQ G+ V+++K+
Sbjct: 480  AKMALTMILQRFTFDLSPTYAHAPFTVLTLQPQHGAQVVFRKI 522


>dbj|BAL45206.1| cytochrome P450 monooxygenase [Glycyrrhiza uralensis]
          Length = 522

 Score =  575 bits (1483), Expect = e-161
 Identities = 276/503 (54%), Positives = 378/503 (75%)
 Frame = -2

Query: 2008 WRILSWAWFTPKSIEKRLRKQGFRGNPYRFLVGDIKDSTMMLQKAMAKPIPLCNDIVARI 1829
            WR+L+W W  PK +E+ LR+QG +GNPYR +VGD+KD   M ++A +KP+ L +DI+ R+
Sbjct: 25   WRMLNWLWLRPKKLERLLREQGLQGNPYRLIVGDLKDLMKMRKEAKSKPMNLSDDILPRV 84

Query: 1828 MPHVDHTIKTYGRNSFTWVGRIPRIHVMDPDLIREVLTHSTKYVKNFDIHDPLVKFLLTG 1649
             P+V  ++  +G+NSF W G IPR+ + DP+ I++VL     + K+    D LVK L TG
Sbjct: 85   FPYVQQSVTKHGKNSFIWFGPIPRVTLTDPEQIKDVLNKIYDFPKSDG--DRLVKLLATG 142

Query: 1648 VGSFEGEKWSRHRRIISPAFTLEKLKKMLPAFAVCYDDLLNKWENTCTREGSIEVDIFPT 1469
            + S+EGEKWS+HRRII+PAF LEKLK MLP F    +DL+ KWE   + +GS E+DI+P 
Sbjct: 143  LVSYEGEKWSKHRRIINPAFNLEKLKIMLPIFFQSCNDLITKWEGMLSSDGSCEMDIWPF 202

Query: 1468 FDVLTSDVISKVAFDSTYEESGRTFRLLKELMDLTMEAIREVYIPGWSYLPTKRNRRMKL 1289
               L SDVI++ AF S+YEE  R F+L KE ++ TM  + +VYIPGW +LPT  +RRMK 
Sbjct: 203  LQNLASDVIARAAFGSSYEEGRRIFQLQKEQVEHTMSVLVKVYIPGWRFLPTTTHRRMKK 262

Query: 1288 INHEMREFLRNIIDKRVKARKAGELPEEEDLLGVLLESNFQEIEKQGNDHKQNVGMSIDD 1109
            I+ E++  L+ +I+KR +A KAGE   + +LL +LLESN +E+E+ GN+  +NVGMS++D
Sbjct: 263  IDREIKASLKEMINKRERALKAGE-ATKNNLLDILLESNHKEVEEHGNN--RNVGMSLED 319

Query: 1108 VIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERARAEVFQAFGNKKPDDFEKLNHLK 929
            VIEECKLFYFAGQETT  LL WT +LLS++P+WQ RAR EV Q FGNKKP DF+ L+HLK
Sbjct: 320  VIEECKLFYFAGQETTSGLLVWTMVLLSRYPDWQARAREEVLQVFGNKKP-DFDGLSHLK 378

Query: 928  CVSMILYEVLRLYPPVIDLTKIVHEDTKLGPYTIPGGTQVMMPSVMIHREKSIWGEDAME 749
             V+MILYEVLRLYPPVI L + VH+D KLG  T+P G Q+ +P V+ H ++ +WG+DA  
Sbjct: 379  IVTMILYEVLRLYPPVIALARTVHKDVKLGNRTLPAGVQLYLPIVLFHHDRELWGDDAEV 438

Query: 748  FNPERFADGVANATKNGALAYLPFSWGPRVCLGQNFALLQAKLGLVMILQRFSFDVSPSY 569
            FNPERF++GV  AT NG +++ PF WGPR+C+GQNF+LL+AK+ + MILQ FSF++SP+Y
Sbjct: 439  FNPERFSEGVLKAT-NGKVSFFPFGWGPRICVGQNFSLLEAKMAVSMILQHFSFELSPAY 497

Query: 568  AHAPYTVLTLQPQFGSHVIYKKL 500
            AHAP TV+TL+PQ+G+H+I +K+
Sbjct: 498  AHAPTTVITLRPQYGAHIILRKV 520