BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cephaelis21_contig00000212
         (2338 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAL54888.1|AF116915_1 obtusifoliol-14-demethylase [Nicotiana ...   784   0.0  
gb|AAL40888.1| obtusifoliol-14-demethylase [Nicotiana tabacum]        778   0.0  
emb|CAL69911.1| obtusifoliol-14-demethylase [Petunia x hybrida]       777   0.0  
gb|ADN39418.1| obtusifoliol-14-demethylase [Withania somnifera]       773   0.0  
ref|NP_001234537.1| obtusifoliol 14alpha-demethylase [Solanum ly...   773   0.0  

>gb|AAL54888.1|AF116915_1 obtusifoliol-14-demethylase [Nicotiana tabacum]
          Length = 487

 Score =  784 bits (2024), Expect = 0.0
 Identities = 380/456 (83%), Positives = 413/456 (90%)
 Frame = +2

Query: 467  IMPRSKKRLPPVVKARPPLIGGLLRFFSGPVVMLREEYPKLGSVFSLRLLNKNITFFIGP 646
            IMPRSKKRLPPV+K+ P ++GGLLRF  GPVVMLREEYPKLGSVF+L LLNKNITFF+GP
Sbjct: 29   IMPRSKKRLPPVIKSWP-ILGGLLRFLKGPVVMLREEYPKLGSVFTLNLLNKNITFFVGP 87

Query: 647  EVSAHFFKAPESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTESLRVNKLRGYV 826
            EVSAHFFKAPE+DLSQQEVYQFNVPTFGPGVVFDVDY+IRQEQFRFFTESLRVNKL+GYV
Sbjct: 88   EVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVFDVDYTIRQEQFRFFTESLRVNKLKGYV 147

Query: 827  DQMVLEAQDYFSKWGDTGEVDLKYELEHLIILTASRCLLGEEVRNKLFDDVSALFHDLDN 1006
            DQMV+EA++YFSKWGD+GEVDLKYELEHLIILTASRCLLGEEVRNKLF+DVSALFHDLDN
Sbjct: 148  DQMVMEAEEYFSKWGDSGEVDLKYELEHLIILTASRCLLGEEVRNKLFEDVSALFHDLDN 207

Query: 1007 GMLPISVIFPYLPIPAHHRRDKARKKLADIFAGIIDSRRQSGKTQNDMLQCFMDSKYKDG 1186
            GMLPISVIFPYLPIPAH RRD ARKKLA+IFA IIDSR+++GK ++DMLQCF+DSKYKDG
Sbjct: 208  GMLPISVIFPYLPIPAHRRRDNARKKLAEIFANIIDSRKRTGKAESDMLQCFIDSKYKDG 267

Query: 1187 RPTTQAEVTGLLIAALFAGQHTSSITSAWTGAYLLCNPKYMSQVLDEQKKLMKQHGNKVD 1366
            R TT++E+TGLLIAALFAGQHTSSITS W GAYLLCN KYMS V+DEQK LMK+HGNKVD
Sbjct: 268  RATTESEITGLLIAALFAGQHTSSITSTWAGAYLLCNNKYMSAVVDEQKNLMKKHGNKVD 327

Query: 1367 HDILSEMEVLYRCIKEAXXXXXXXXXXXXXXXSDFTVTTKEGKEYDIPKGNIVATSPAFA 1546
            HDILSEMEVLYRCIKEA               SDFTV T+EGKEYDIPKG+IVATSPAFA
Sbjct: 328  HDILSEMEVLYRCIKEALRLHPPLIMLLRSSHSDFTVKTREGKEYDIPKGHIVATSPAFA 387

Query: 1547 NRLPHIYKDPDTYDPDRFAPGREEDKVAGAFSYISFGGGRHGCLGEPFAYLQIKAIWSHL 1726
            NRLPH+YK+PDTYDPDRFAPGR+EDKVAGAFSYISFGGGRHGCLGEPFAYLQIKAIWSHL
Sbjct: 388  NRLPHVYKNPDTYDPDRFAPGRDEDKVAGAFSYISFGGGRHGCLGEPFAYLQIKAIWSHL 447

Query: 1727 LRNFELELISPFPEIDWNAXXXXXXXXXXXRYKRRQ 1834
            LRNFE ELISPFPEIDWNA           +YKRR+
Sbjct: 448  LRNFEFELISPFPEIDWNAMVVGVKGKVMVKYKRRK 483


>gb|AAL40888.1| obtusifoliol-14-demethylase [Nicotiana tabacum]
          Length = 487

 Score =  778 bits (2009), Expect = 0.0
 Identities = 377/456 (82%), Positives = 410/456 (89%)
 Frame = +2

Query: 467  IMPRSKKRLPPVVKARPPLIGGLLRFFSGPVVMLREEYPKLGSVFSLRLLNKNITFFIGP 646
            IMPRSKKRLPPV+K+ P ++GGLLRF  GPVVMLREEYPKLGSVF+L LLNKNITFFIGP
Sbjct: 29   IMPRSKKRLPPVIKSWP-ILGGLLRFLKGPVVMLREEYPKLGSVFTLNLLNKNITFFIGP 87

Query: 647  EVSAHFFKAPESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTESLRVNKLRGYV 826
            EVSAHFFKAPE+DLSQQEVYQFNVPTFGPGVVFDVDY+IRQEQFRFFTE+LRVNKL+GYV
Sbjct: 88   EVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVFDVDYTIRQEQFRFFTEALRVNKLKGYV 147

Query: 827  DQMVLEAQDYFSKWGDTGEVDLKYELEHLIILTASRCLLGEEVRNKLFDDVSALFHDLDN 1006
            D MV+EA++YFSKWGD+GE+DLKYELEHLIILTASRCLLGEEVRNKLF+DVSALFHDLDN
Sbjct: 148  DHMVMEAEEYFSKWGDSGEMDLKYELEHLIILTASRCLLGEEVRNKLFEDVSALFHDLDN 207

Query: 1007 GMLPISVIFPYLPIPAHHRRDKARKKLADIFAGIIDSRRQSGKTQNDMLQCFMDSKYKDG 1186
            GMLPISVIFPYLPIPAH RRD ARKKLA+IFA IIDSR+++GK ++DMLQCF+DSKYKDG
Sbjct: 208  GMLPISVIFPYLPIPAHRRRDNARKKLAEIFANIIDSRKRTGKAESDMLQCFIDSKYKDG 267

Query: 1187 RPTTQAEVTGLLIAALFAGQHTSSITSAWTGAYLLCNPKYMSQVLDEQKKLMKQHGNKVD 1366
            R TT +E+TGLLIAALFAGQHTSSITS WTGAYLLCN KYMS V+DEQK LMK+HGNKVD
Sbjct: 268  RATTDSEITGLLIAALFAGQHTSSITSTWTGAYLLCNNKYMSAVVDEQKNLMKKHGNKVD 327

Query: 1367 HDILSEMEVLYRCIKEAXXXXXXXXXXXXXXXSDFTVTTKEGKEYDIPKGNIVATSPAFA 1546
            HDILSEMEVLYRCIKE                SDFTV T+EGKEYDIPKG+IVATSPAFA
Sbjct: 328  HDILSEMEVLYRCIKEVLRLHPPLIMLLRSSHSDFTVKTREGKEYDIPKGHIVATSPAFA 387

Query: 1547 NRLPHIYKDPDTYDPDRFAPGREEDKVAGAFSYISFGGGRHGCLGEPFAYLQIKAIWSHL 1726
            NRLPH+YK+PDTYDPDRF PGR+EDKVAGAFSYISFGGGRHGCLGEPFAYLQIKAIWSHL
Sbjct: 388  NRLPHVYKNPDTYDPDRFTPGRDEDKVAGAFSYISFGGGRHGCLGEPFAYLQIKAIWSHL 447

Query: 1727 LRNFELELISPFPEIDWNAXXXXXXXXXXXRYKRRQ 1834
            LRNFE ELISPFPEIDWNA           +YKRR+
Sbjct: 448  LRNFEFELISPFPEIDWNAMVVGVKGKVMVKYKRRK 483


>emb|CAL69911.1| obtusifoliol-14-demethylase [Petunia x hybrida]
          Length = 492

 Score =  777 bits (2007), Expect = 0.0
 Identities = 386/488 (79%), Positives = 413/488 (84%)
 Frame = +2

Query: 383  EAAGPNKFXXXXXXXXXXXXXXXXXXXXIMPRSKKRLPPVVKARPPLIGGLLRFFSGPVV 562
            E AG NK                     IMPRSKKRLPP VKA P ++GGL RF  GPVV
Sbjct: 2    ELAGDNKIMNVGLLLVATLVVAKLISALIMPRSKKRLPPSVKAWP-VLGGLFRFLKGPVV 60

Query: 563  MLREEYPKLGSVFSLRLLNKNITFFIGPEVSAHFFKAPESDLSQQEVYQFNVPTFGPGVV 742
            MLREEYPKLGSVF+L LLNK ITFFIGPEVSAHFFKAPE+DLSQQEVYQFNVPTFGPGVV
Sbjct: 61   MLREEYPKLGSVFTLNLLNKKITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVV 120

Query: 743  FDVDYSIRQEQFRFFTESLRVNKLRGYVDQMVLEAQDYFSKWGDTGEVDLKYELEHLIIL 922
            FDVDY+IRQEQFRFFTESLRV KL+GYVDQMV+EA++YFSKWGD+GEVDLKYELEHLIIL
Sbjct: 121  FDVDYTIRQEQFRFFTESLRVTKLKGYVDQMVMEAEEYFSKWGDSGEVDLKYELEHLIIL 180

Query: 923  TASRCLLGEEVRNKLFDDVSALFHDLDNGMLPISVIFPYLPIPAHHRRDKARKKLADIFA 1102
            TASRCLLGEEVRNKLF+DVSALFHDLDNGMLPISVIFPYL IPAH RRD ARKKLA IFA
Sbjct: 181  TASRCLLGEEVRNKLFEDVSALFHDLDNGMLPISVIFPYLSIPAHRRRDNARKKLAQIFA 240

Query: 1103 GIIDSRRQSGKTQNDMLQCFMDSKYKDGRPTTQAEVTGLLIAALFAGQHTSSITSAWTGA 1282
             II SR+++GK ++DMLQCF+DSKYKDGRPTT++E+TGLLIAALFAGQHTSSITS WTGA
Sbjct: 241  NIIASRKRTGKAESDMLQCFIDSKYKDGRPTTESEITGLLIAALFAGQHTSSITSTWTGA 300

Query: 1283 YLLCNPKYMSQVLDEQKKLMKQHGNKVDHDILSEMEVLYRCIKEAXXXXXXXXXXXXXXX 1462
            YLLCN KYMS V+DEQK LMK+HGNKVDHDILSEMEVLYRCIKEA               
Sbjct: 301  YLLCNNKYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSH 360

Query: 1463 SDFTVTTKEGKEYDIPKGNIVATSPAFANRLPHIYKDPDTYDPDRFAPGREEDKVAGAFS 1642
            SDFTV T+EGKEYDIPKG+IVATSPAFANRLPHIYK+PDTYDPDRFAPGREEDK AGAFS
Sbjct: 361  SDFTVKTREGKEYDIPKGHIVATSPAFANRLPHIYKNPDTYDPDRFAPGREEDKTAGAFS 420

Query: 1643 YISFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPEIDWNAXXXXXXXXXXXRY 1822
            YISFGGGRHGCLGEPFAYLQIKAIWSHLLRNFE ELISPFPEIDWNA           +Y
Sbjct: 421  YISFGGGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAMVVGVKGEVMVKY 480

Query: 1823 KRRQPPVD 1846
            KRR+   D
Sbjct: 481  KRRKLSTD 488


>gb|ADN39418.1| obtusifoliol-14-demethylase [Withania somnifera]
          Length = 487

 Score =  773 bits (1997), Expect = 0.0
 Identities = 376/456 (82%), Positives = 408/456 (89%)
 Frame = +2

Query: 467  IMPRSKKRLPPVVKARPPLIGGLLRFFSGPVVMLREEYPKLGSVFSLRLLNKNITFFIGP 646
            IMPRSKKRLPPV+ + P ++GGL+RF  GPVVMLREEYPKLGSVF+L +LNKNITFFIGP
Sbjct: 29   IMPRSKKRLPPVINSWP-ILGGLIRFLKGPVVMLREEYPKLGSVFTLNILNKNITFFIGP 87

Query: 647  EVSAHFFKAPESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTESLRVNKLRGYV 826
            EVSAHFFKAPE+DLSQQEVYQFNVPTFGPGVVFDVDY+IRQEQF FFTE+LRV KL+GYV
Sbjct: 88   EVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVFDVDYTIRQEQFTFFTEALRVTKLKGYV 147

Query: 827  DQMVLEAQDYFSKWGDTGEVDLKYELEHLIILTASRCLLGEEVRNKLFDDVSALFHDLDN 1006
            DQMV+EA++YFSKWGD+GEVDLKYELEHLIILTASRCLLG+EV NKLF DVSALFHDLDN
Sbjct: 148  DQMVMEAEEYFSKWGDSGEVDLKYELEHLIILTASRCLLGQEVPNKLFADVSALFHDLDN 207

Query: 1007 GMLPISVIFPYLPIPAHHRRDKARKKLADIFAGIIDSRRQSGKTQNDMLQCFMDSKYKDG 1186
            GMLPISVIFPYLPIPAH RRDKARKKLA+IFA II+SR+++GK +NDMLQCF+DSKYKDG
Sbjct: 208  GMLPISVIFPYLPIPAHRRRDKARKKLAEIFANIINSRKRTGKAENDMLQCFIDSKYKDG 267

Query: 1187 RPTTQAEVTGLLIAALFAGQHTSSITSAWTGAYLLCNPKYMSQVLDEQKKLMKQHGNKVD 1366
            R TT+ E+TGLLIAALFAGQHTSSITS WTGAYLLCN KYMS V+DEQK LMK+HGNKVD
Sbjct: 268  RATTEGEITGLLIAALFAGQHTSSITSTWTGAYLLCNKKYMSAVVDEQKNLMKKHGNKVD 327

Query: 1367 HDILSEMEVLYRCIKEAXXXXXXXXXXXXXXXSDFTVTTKEGKEYDIPKGNIVATSPAFA 1546
            HDILSEMEVLYRCIKEA               SDF VTTKEGKEYDIPKG+IVATSPAFA
Sbjct: 328  HDILSEMEVLYRCIKEALRLHPPLIMLLRHSHSDFCVTTKEGKEYDIPKGHIVATSPAFA 387

Query: 1547 NRLPHIYKDPDTYDPDRFAPGREEDKVAGAFSYISFGGGRHGCLGEPFAYLQIKAIWSHL 1726
            NRLPHIYK+PDTYDPDRF+PGREEDK AGAFSYISFGGGRHGCLGEPFAYLQIKAIWSHL
Sbjct: 388  NRLPHIYKNPDTYDPDRFSPGREEDKAAGAFSYISFGGGRHGCLGEPFAYLQIKAIWSHL 447

Query: 1727 LRNFELELISPFPEIDWNAXXXXXXXXXXXRYKRRQ 1834
            LRNFE ELISPFPEIDWNA           +YKRR+
Sbjct: 448  LRNFEFELISPFPEIDWNAMVVGVKGEVMVKYKRRK 483


>ref|NP_001234537.1| obtusifoliol 14alpha-demethylase [Solanum lycopersicum]
            gi|299735181|gb|ADJ37071.1| obtusifoliol
            14alpha-demethylase [Solanum lycopersicum]
          Length = 487

 Score =  773 bits (1997), Expect = 0.0
 Identities = 377/460 (81%), Positives = 411/460 (89%)
 Frame = +2

Query: 467  IMPRSKKRLPPVVKARPPLIGGLLRFFSGPVVMLREEYPKLGSVFSLRLLNKNITFFIGP 646
            IMPRSKKRLPPVVKA P ++GGL+RF  GPVVMLR+EYPKLGSVF+L LLNKNITFFIGP
Sbjct: 29   IMPRSKKRLPPVVKAWP-IVGGLIRFLKGPVVMLRQEYPKLGSVFTLNLLNKNITFFIGP 87

Query: 647  EVSAHFFKAPESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTESLRVNKLRGYV 826
            EVSAHFFKAPE+DLSQQEVYQFNVPTFGPGVVFDVDY+IRQEQFRFFTE+LRV KL+GYV
Sbjct: 88   EVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVFDVDYTIRQEQFRFFTEALRVTKLKGYV 147

Query: 827  DQMVLEAQDYFSKWGDTGEVDLKYELEHLIILTASRCLLGEEVRNKLFDDVSALFHDLDN 1006
            DQMV EA++YFSKWG++GEVDLKYELEHLIILTASRCLLGEEVRNKLFDDVSALFHDLDN
Sbjct: 148  DQMVTEAEEYFSKWGESGEVDLKYELEHLIILTASRCLLGEEVRNKLFDDVSALFHDLDN 207

Query: 1007 GMLPISVIFPYLPIPAHHRRDKARKKLADIFAGIIDSRRQSGKTQNDMLQCFMDSKYKDG 1186
            GMLPISVIFPYLPIPAH RRD ARKKLA+IFA II+SR+++GK +NDMLQCF+DSKYKDG
Sbjct: 208  GMLPISVIFPYLPIPAHRRRDNARKKLAEIFANIINSRKRTGKAENDMLQCFIDSKYKDG 267

Query: 1187 RPTTQAEVTGLLIAALFAGQHTSSITSAWTGAYLLCNPKYMSQVLDEQKKLMKQHGNKVD 1366
            RPTT+ E+TGLLIAALFAGQHTSSITS WTG+YLL N KYMS V+DEQK LMK+HGNKVD
Sbjct: 268  RPTTEGEITGLLIAALFAGQHTSSITSTWTGSYLLTNDKYMSAVVDEQKNLMKKHGNKVD 327

Query: 1367 HDILSEMEVLYRCIKEAXXXXXXXXXXXXXXXSDFTVTTKEGKEYDIPKGNIVATSPAFA 1546
            HDILSEMEVLYRCIKEA               S+F+VTT+EGKEYDI KG+IVATSPAFA
Sbjct: 328  HDILSEMEVLYRCIKEALRLHPPLIMLLRSSHSEFSVTTREGKEYDISKGHIVATSPAFA 387

Query: 1547 NRLPHIYKDPDTYDPDRFAPGREEDKVAGAFSYISFGGGRHGCLGEPFAYLQIKAIWSHL 1726
            NRLPHI+K+PDTYDPDRF PGREEDK AGAFSYISFGGGRHGCLGEPFAYLQIKAIWSHL
Sbjct: 388  NRLPHIFKNPDTYDPDRFGPGREEDKAAGAFSYISFGGGRHGCLGEPFAYLQIKAIWSHL 447

Query: 1727 LRNFELELISPFPEIDWNAXXXXXXXXXXXRYKRRQPPVD 1846
            LRNFE ELISPFPEIDWNA           +YKRR+  V+
Sbjct: 448  LRNFEFELISPFPEIDWNAMVVGVKGEVMVKYKRRKLSVE 487