BLASTX nr result
ID: Catharanthus23_contig00028667
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Catharanthus23_contig00028667
(695 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004243967.1| PREDICTED: uncharacterized protein LOC101253... 370 e-100
ref|XP_006346024.1| PREDICTED: uncharacterized protein LOC102604... 364 1e-98
ref|XP_002266113.2| PREDICTED: uncharacterized protein LOC100254... 322 7e-86
ref|XP_004139838.1| PREDICTED: uncharacterized protein LOC101215... 316 4e-84
ref|XP_002513329.1| conserved hypothetical protein [Ricinus comm... 316 4e-84
gb|EPS74207.1| hypothetical protein M569_00548, partial [Genlise... 315 1e-83
gb|EXB62639.1| hypothetical protein L484_023934 [Morus notabilis] 312 7e-83
ref|XP_006493762.1| PREDICTED: uncharacterized protein LOC102629... 306 3e-81
ref|XP_006422271.1| hypothetical protein CICLE_v10006846mg [Citr... 305 7e-81
ref|XP_002316147.1| hypothetical protein POPTR_0010s17920g [Popu... 300 2e-79
gb|EOY14197.1| Vacuole, catalytic [Theobroma cacao] 299 5e-79
ref|XP_002311290.2| hypothetical protein POPTR_0008s08370g [Popu... 290 3e-76
ref|XP_004308235.1| PREDICTED: uncharacterized protein LOC101294... 290 4e-76
ref|XP_003532919.1| PREDICTED: uncharacterized protein LOC100798... 287 3e-75
gb|EMJ13701.1| hypothetical protein PRUPE_ppa017705mg [Prunus pe... 284 2e-74
gb|AAD34680.1|AC006341_8 Contains a PF|01464 Transglycosylase SL... 284 2e-74
ref|NP_173079.2| uncharacterized protein [Arabidopsis thaliana] ... 284 2e-74
ref|XP_006307632.1| hypothetical protein CARUB_v10009260mg [Caps... 283 3e-74
ref|XP_002892901.1| hypothetical protein ARALYDRAFT_312606 [Arab... 282 8e-74
ref|NP_001046030.1| Os02g0170900 [Oryza sativa Japonica Group] g... 258 1e-66
>ref|XP_004243967.1| PREDICTED: uncharacterized protein LOC101253506 [Solanum
lycopersicum]
Length = 422
Score = 370 bits (950), Expect = e-100
Identities = 181/231 (78%), Positives = 201/231 (87%)
Frame = -1
Query: 695 EVFESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWR 516
EV SKLLYKPFVNVYLGAAYLKWLSN+EQKERSEEFMVRAYKGGT KA HKSTL +WR
Sbjct: 129 EVTMDSKLLYKPFVNVYLGAAYLKWLSNYEQKERSEEFMVRAYKGGTKKATHKSTLPFWR 188
Query: 515 RYLSVKESLPSRKVFEVIPMPNXXXXXXXXPHTKDRPVNTTWDSRTSKEDMEEIWNNPIV 336
YLSVKE+LPSRK+F+V P+P K PVNTTWDSRTS EDMEE+WN+P V
Sbjct: 189 SYLSVKETLPSRKIFDVNPLPPTASATGVPE--KKGPVNTTWDSRTSAEDMEEMWNHPCV 246
Query: 335 SKEWTKSGEKKGKVRFSHDTEKRPYLSRVELKAVSEIILSKHFSTKGVKPSVLCAIAEIV 156
S EW+KSGEK+G VRFSHDTEKRPYLSRVEL+AV+E+I+SK+FST+G+KP+VLCA+AEIV
Sbjct: 247 STEWSKSGEKRGHVRFSHDTEKRPYLSRVELRAVAEVIVSKYFSTRGLKPTVLCAVAEIV 306
Query: 155 SMRFVNGIGQRTGLMGIDYPTARWLYKDLGYKAYIVESVEDLTKPFVSMCF 3
+MRFV GIGQRTGLMGIDYPTARWLYKDLGYK Y VESVEDLTKPFVSM F
Sbjct: 307 TMRFVEGIGQRTGLMGIDYPTARWLYKDLGYKGYKVESVEDLTKPFVSMYF 357
Score = 87.0 bits (214), Expect = 5e-15
Identities = 54/137 (39%), Positives = 77/137 (56%), Gaps = 9/137 (6%)
Frame = -1
Query: 392 WDSRTSKEDMEEIWNNPIVSKEWTKSGEKKG-KVRFSHDTEKRPYLSRVELKAVSEIILS 216
WD D+E +W +P V EW +GE KG KV S D + +PYL++ E+KAV+ II+
Sbjct: 9 WDDCVDPLDLEAMWLDPEVRAEWLNAGETKGSKVHLSRDPDGQPYLTQTEMKAVAAIIVR 68
Query: 215 KHFSTKGVKPSVLCAIAEIVSMRFVNGIGQR-------TGLMGIDYPTARWLYKDLGYKA 57
+HF ++ + +LCAIAEI S R + G+M I TA WL DLGY+
Sbjct: 69 RHFLSQ-IDLDMLCAIAEIESDRQLLATRYNKKSKEITMGIMQILPKTADWLVSDLGYRT 127
Query: 56 Y-IVESVEDLTKPFVSM 9
Y + + L KPFV++
Sbjct: 128 YEVTMDSKLLYKPFVNV 144
Score = 60.5 bits (145), Expect = 5e-07
Identities = 28/59 (47%), Positives = 39/59 (66%)
Frame = -1
Query: 686 ESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWRRY 510
ES + L KPFV++Y GAAY+ WLS +E +ERS +F+V+AY G N + T W R+
Sbjct: 343 ESVEDLTKPFVSMYFGAAYVAWLSEYEGRERSLQFLVQAYLAGPQNVNLQETGPMWLRF 401
>ref|XP_006346024.1| PREDICTED: uncharacterized protein LOC102604381 [Solanum tuberosum]
Length = 422
Score = 364 bits (935), Expect = 1e-98
Identities = 178/231 (77%), Positives = 199/231 (86%)
Frame = -1
Query: 695 EVFESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWR 516
EV SK LYKPFVNVYLGAAYLKWLSN+EQKERSEEF VRAYKGGT KA HKSTL +WR
Sbjct: 129 EVTMDSKFLYKPFVNVYLGAAYLKWLSNYEQKERSEEFTVRAYKGGTKKATHKSTLPFWR 188
Query: 515 RYLSVKESLPSRKVFEVIPMPNXXXXXXXXPHTKDRPVNTTWDSRTSKEDMEEIWNNPIV 336
YLSVKE+LPSRK+F+V +P K PVNTTWDSRTS EDMEE+WN+P V
Sbjct: 189 SYLSVKETLPSRKIFDVNTLPPTASATGIPE--KKGPVNTTWDSRTSAEDMEEMWNHPYV 246
Query: 335 SKEWTKSGEKKGKVRFSHDTEKRPYLSRVELKAVSEIILSKHFSTKGVKPSVLCAIAEIV 156
SKEW+K+GEK+G VRFSHDTEKRPYLSRVEL+AV+E+I+SK+FST+G+KP+VLCA+AEIV
Sbjct: 247 SKEWSKTGEKRGHVRFSHDTEKRPYLSRVELRAVAEVIVSKYFSTRGLKPTVLCAVAEIV 306
Query: 155 SMRFVNGIGQRTGLMGIDYPTARWLYKDLGYKAYIVESVEDLTKPFVSMCF 3
+MRFV GIGQRTGLMGIDYPTARWLYKDLGYK Y VESVEDLTKPFVSM F
Sbjct: 307 TMRFVEGIGQRTGLMGIDYPTARWLYKDLGYKTYKVESVEDLTKPFVSMYF 357
Score = 87.0 bits (214), Expect = 5e-15
Identities = 53/137 (38%), Positives = 77/137 (56%), Gaps = 9/137 (6%)
Frame = -1
Query: 392 WDSRTSKEDMEEIWNNPIVSKEWTKSGEKKG-KVRFSHDTEKRPYLSRVELKAVSEIILS 216
WD D+E +W +P V EW +GE KG KV S D + +PYL++ E+KAV+ II+
Sbjct: 9 WDDCVDPLDLEAMWLDPEVRAEWLNAGETKGSKVHLSRDPDGQPYLTQTEMKAVAGIIVR 68
Query: 215 KHFSTKGVKPSVLCAIAEIVSMRFVNGIGQR-------TGLMGIDYPTARWLYKDLGYKA 57
+HF ++ + +LCAIAE+ S R + G+M I TA WL DLGY+
Sbjct: 69 RHFLSQ-IDSDMLCAIAELESDRQLLATRYNKKSKEITMGIMQILPKTADWLVSDLGYRT 127
Query: 56 Y-IVESVEDLTKPFVSM 9
Y + + L KPFV++
Sbjct: 128 YEVTMDSKFLYKPFVNV 144
Score = 60.5 bits (145), Expect = 5e-07
Identities = 28/59 (47%), Positives = 39/59 (66%)
Frame = -1
Query: 686 ESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWRRY 510
ES + L KPFV++Y GAAY+ WLS +E +ERS +F+V+AY G N + T W R+
Sbjct: 343 ESVEDLTKPFVSMYFGAAYVAWLSEYEGRERSLQFLVQAYLAGPQNVNLQETGPMWLRF 401
>ref|XP_002266113.2| PREDICTED: uncharacterized protein LOC100254368 [Vitis vinifera]
gi|296082756|emb|CBI21761.3| unnamed protein product
[Vitis vinifera]
Length = 422
Score = 322 bits (825), Expect = 7e-86
Identities = 158/231 (68%), Positives = 185/231 (80%)
Frame = -1
Query: 695 EVFESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWR 516
EV + LL++PF+NVYLGAAYLKWLSN++ KERSEEF+VRAYKGG KA HKSTL+YW+
Sbjct: 128 EVEGNPILLFRPFINVYLGAAYLKWLSNYDGKERSEEFVVRAYKGGPKKAAHKSTLEYWK 187
Query: 515 RYLSVKESLPSRKVFEVIPMPNXXXXXXXXPHTKDRPVNTTWDSRTSKEDMEEIWNNPIV 336
RYLSVKESLP RK P PN +T WDSR S EDM+E+WN+P V
Sbjct: 188 RYLSVKESLPFRKSLYDGPYPNDASGSGAPVSVIKGVNHTFWDSRASPEDMDEMWNHPDV 247
Query: 335 SKEWTKSGEKKGKVRFSHDTEKRPYLSRVELKAVSEIILSKHFSTKGVKPSVLCAIAEIV 156
KEW+K GE +GKV FSHD EKRPYLSRVE+KAV+EIILSKHFST+ VK ++LCA+AEIV
Sbjct: 248 LKEWSKCGEIRGKVLFSHDKEKRPYLSRVEVKAVAEIILSKHFSTRQVKSTILCALAEIV 307
Query: 155 SMRFVNGIGQRTGLMGIDYPTARWLYKDLGYKAYIVESVEDLTKPFVSMCF 3
SMRFVNG+G RTG+MGIDYPTA WLY+DLGYKAY VE+V+DLTKPF+SM F
Sbjct: 308 SMRFVNGVGARTGIMGIDYPTAMWLYRDLGYKAYRVEAVDDLTKPFISMYF 358
Score = 86.7 bits (213), Expect = 7e-15
Identities = 50/137 (36%), Positives = 78/137 (56%), Gaps = 9/137 (6%)
Frame = -1
Query: 392 WDSRTSKEDMEEIWNNPIVSKEWTKSGEKKG-KVRFSHDTEKRPYLSRVELKAVSEIILS 216
WD D+E +W +P VS EW GE +G KV S D + PYL+++E+KAV+ I++S
Sbjct: 8 WDDCVDPRDLEALWLDPDVSTEWLDVGETRGRKVHLSRDPDGEPYLTQIEMKAVAAIVVS 67
Query: 215 KHFSTKGVKPSVLCAIAEIVSMR-------FVNGIGQRTGLMGIDYPTARWLYKDLGYKA 57
+HF ++ + ++CAI+EI S R G+M + TA WL ++LGY+
Sbjct: 68 RHFDSQ-IDLEMICAISEIESDRQPLAEQYDKKAKDTTLGIMQLLPKTADWLVRELGYRT 126
Query: 56 YIVESVEDLT-KPFVSM 9
Y VE L +PF+++
Sbjct: 127 YEVEGNPILLFRPFINV 143
Score = 57.0 bits (136), Expect = 6e-06
Identities = 23/54 (42%), Positives = 36/54 (66%)
Frame = -1
Query: 671 LYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWRRY 510
L KPF+++Y GAAYL WLS +E ++R+ +F+V+AY G N + T +W +
Sbjct: 349 LTKPFISMYFGAAYLAWLSEYEGRQRTPQFIVQAYLAGPKNVNLQETGHHWLNF 402
>ref|XP_004139838.1| PREDICTED: uncharacterized protein LOC101215745 [Cucumis sativus]
Length = 423
Score = 316 bits (810), Expect = 4e-84
Identities = 152/225 (67%), Positives = 178/225 (79%)
Frame = -1
Query: 677 KLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWRRYLSVK 498
++L KPFV+VY GAAYLKWLSNFEQKERSEEF+VRAY+GGT KA HK+TL YW+RYLSVK
Sbjct: 134 EVLSKPFVSVYFGAAYLKWLSNFEQKERSEEFVVRAYRGGTKKATHKTTLPYWKRYLSVK 193
Query: 497 ESLPSRKVFEVIPMPNXXXXXXXXPHTKDRPVNTTWDSRTSKEDMEEIWNNPIVSKEWTK 318
ESLPSRK + T WD R + EDMEE+WNNP V KEWTK
Sbjct: 194 ESLPSRKHINEVSTSTTSPPSASGNTEGAAITYTFWDCRATPEDMEEMWNNPDVQKEWTK 253
Query: 317 SGEKKGKVRFSHDTEKRPYLSRVELKAVSEIILSKHFSTKGVKPSVLCAIAEIVSMRFVN 138
SGEKKG VRFSHD +KRPY+SRVELKA++EIILSKHFSTKGV+P+VLCA+AE+VSMRF+N
Sbjct: 254 SGEKKGNVRFSHDLKKRPYVSRVELKAIAEIILSKHFSTKGVQPTVLCALAEVVSMRFIN 313
Query: 137 GIGQRTGLMGIDYPTARWLYKDLGYKAYIVESVEDLTKPFVSMCF 3
G+G R G+MGIDY TA WLY +L Y+AY ++S +DLTKPFVSM F
Sbjct: 314 GVGARPGIMGIDYSTAFWLYMELSYRAYRLDSTDDLTKPFVSMYF 358
Score = 92.0 bits (227), Expect = 2e-16
Identities = 53/139 (38%), Positives = 84/139 (60%), Gaps = 9/139 (6%)
Frame = -1
Query: 392 WDSRTSKEDMEEIWNNPIVSKEWTKSGEKKG-KVRFSHDTEKRPYLSRVELKAVSEIILS 216
WD +DME +W+ P V EW +GE K KV S D + +PYL++ E+KAV++I++
Sbjct: 8 WDDCVDPQDMEAMWSYPQVCAEWLDAGESKTQKVHLSRDPDGQPYLTQTEMKAVADIVVH 67
Query: 215 KHFSTKGVKPSVLCAIAEI------VSMRFVNGIGQRT-GLMGIDYPTARWLYKDLGYKA 57
+HF + V ++CA+AE+ ++ R+ + T G+M I TA WL +L Y++
Sbjct: 68 RHFGS-NVDSDMICALAELESDRQPLATRYDKKNKESTLGIMQITLKTAEWLVSELRYQS 126
Query: 56 YIVE-SVEDLTKPFVSMCF 3
Y +E + E L+KPFVS+ F
Sbjct: 127 YGLEGNPEVLSKPFVSVYF 145
Score = 56.6 bits (135), Expect = 7e-06
Identities = 25/59 (42%), Positives = 39/59 (66%)
Frame = -1
Query: 686 ESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWRRY 510
+S+ L KPFV++Y GAAYL WLS++E +ER+ +F+V+AY G + T W ++
Sbjct: 344 DSTDDLTKPFVSMYFGAAYLAWLSDYEGRERTRQFVVQAYIAGPQNVDLPETGPLWLKF 402
>ref|XP_002513329.1| conserved hypothetical protein [Ricinus communis]
gi|223547237|gb|EEF48732.1| conserved hypothetical
protein [Ricinus communis]
Length = 429
Score = 316 bits (810), Expect = 4e-84
Identities = 156/238 (65%), Positives = 187/238 (78%), Gaps = 7/238 (2%)
Frame = -1
Query: 695 EVFESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWR 516
+V E+ +LY+PFV+VY GAAYLKWLSNFEQKERSEEF+VRAYKGG KA HKSTL YW+
Sbjct: 128 DVEENPDILYRPFVSVYFGAAYLKWLSNFEQKERSEEFVVRAYKGGIKKATHKSTLHYWK 187
Query: 515 RYLSVKESLPSRKVFEVIPMPNXXXXXXXXPHTKDRPVNTT-------WDSRTSKEDMEE 357
RYLSVKESLP R+ + P N + + WDS+TS EDM E
Sbjct: 188 RYLSVKESLPFRRFVDDGPSVNPCCPPSAPALAASQNTASNSDGQYVHWDSKTSPEDMLE 247
Query: 356 IWNNPIVSKEWTKSGEKKGKVRFSHDTEKRPYLSRVELKAVSEIILSKHFSTKGVKPSVL 177
+W+N V+KEWTKSGE++GKVRFSHD +KRPYLSR+E+KAV+EIILSK+FST+ VKPS+L
Sbjct: 248 MWSNADVTKEWTKSGERRGKVRFSHDKDKRPYLSRIEVKAVAEIILSKYFSTRAVKPSIL 307
Query: 176 CAIAEIVSMRFVNGIGQRTGLMGIDYPTARWLYKDLGYKAYIVESVEDLTKPFVSMCF 3
CA+AE+VSMRFVNG+G RTGLMGIDY TA WLY +LGY+AY V+SV+DLTKPFVSM F
Sbjct: 308 CALAEMVSMRFVNGVGPRTGLMGIDYSTAFWLYMELGYRAYRVDSVDDLTKPFVSMYF 365
Score = 103 bits (256), Expect = 7e-20
Identities = 58/139 (41%), Positives = 86/139 (61%), Gaps = 9/139 (6%)
Frame = -1
Query: 392 WDSRTSKEDMEEIWNNPIVSKEWTKSGEKKG-KVRFSHDTEKRPYLSRVELKAVSEIILS 216
WD +DME +W P V+ EW +GE KG K+ S D + +PYL++ E+KAV++II+
Sbjct: 8 WDECVDPQDMEAMWREPEVTTEWLDAGEVKGQKIHLSRDPDGQPYLTQTEMKAVADIIVR 67
Query: 215 KHFSTKGVKPSVLCAIAEIVSMRFV--NGIGQRT-----GLMGIDYPTARWLYKDLGYKA 57
++F ++ + P +LCAIAE+ S R + ++T G+M I TA WL +DLGY+
Sbjct: 68 RYFDSQ-ILPDMLCAIAELASDRLLLATNYDKKTKQTTMGIMQILPKTAEWLVRDLGYRI 126
Query: 56 YIVESVED-LTKPFVSMCF 3
Y VE D L +PFVS+ F
Sbjct: 127 YDVEENPDILYRPFVSVYF 145
Score = 56.6 bits (135), Expect = 7e-06
Identities = 25/54 (46%), Positives = 37/54 (68%)
Frame = -1
Query: 671 LYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWRRY 510
L KPFV++Y GAAYL +LS +E +ER+ +F+V+AY GG N + T W ++
Sbjct: 356 LTKPFVSMYFGAAYLAYLSEYEGRERTPQFVVQAYLGGPKNVNLQETGPLWLKF 409
>gb|EPS74207.1| hypothetical protein M569_00548, partial [Genlisea aurea]
Length = 414
Score = 315 bits (806), Expect = 1e-83
Identities = 159/230 (69%), Positives = 181/230 (78%), Gaps = 2/230 (0%)
Frame = -1
Query: 686 ESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWRRYL 507
+ +KLLYKPF+NVYL AA+LKWLS FEQKERSEEFMVRAY GGT KA HKSTL YW++YL
Sbjct: 130 DDTKLLYKPFLNVYLAAAHLKWLSCFEQKERSEEFMVRAYNGGTKKATHKSTLPYWKKYL 189
Query: 506 SVKESLPSRKVFEVIPMPNXXXXXXXXPHTKDR--PVNTTWDSRTSKEDMEEIWNNPIVS 333
VKE LPSR V + + + P+NTTW+SRTS EDMEE+WNN VS
Sbjct: 190 CVKERLPSRLVSFSLVANGSGVPQKKELNFRSLLVPMNTTWESRTSAEDMEEMWNNSEVS 249
Query: 332 KEWTKSGEKKGKVRFSHDTEKRPYLSRVELKAVSEIILSKHFSTKGVKPSVLCAIAEIVS 153
KEW KSGEKKGKVRFSHD EK+PYLSRVEL+AV+EI+ + KPSVLCA++E+VS
Sbjct: 250 KEWIKSGEKKGKVRFSHDLEKQPYLSRVELRAVAEIV--SRMTQFTAKPSVLCALSEMVS 307
Query: 152 MRFVNGIGQRTGLMGIDYPTARWLYKDLGYKAYIVESVEDLTKPFVSMCF 3
MRFVNG GQRTGLMGIDYPTARWLYKDLGY+ Y VESVEDL+KPFVSM F
Sbjct: 308 MRFVNGTGQRTGLMGIDYPTARWLYKDLGYRGYAVESVEDLSKPFVSMYF 357
Score = 81.6 bits (200), Expect = 2e-13
Identities = 47/138 (34%), Positives = 78/138 (56%), Gaps = 10/138 (7%)
Frame = -1
Query: 392 WDSRTSKEDMEEIWNNPIVSKEWTKSGEKKG-KVRFSHDTEKRPYLSRVELKAVSEIILS 216
WD +D++ +W P V EW GE +G KV S D + PYL++ E++AV+ II+
Sbjct: 6 WDDCVDPDDLQAMWLEPEVRTEWIAVGETEGSKVHLSRDPDGHPYLTQTEMRAVAGIIVR 65
Query: 215 KHFSTKGVKPSVLCAIAEIVSMRFVNGIGQ-------RTGLMGIDYPTARWLYKDLGYKA 57
+HF ++ + +LCAIAE+ S R + G+M I A+WL +++G++A
Sbjct: 66 RHFVSQ-IDSDMLCAIAELESDRQPLSTSYNKKSDEVKMGIMQISPTKAQWLIREMGFRA 124
Query: 56 YIV--ESVEDLTKPFVSM 9
Y V + + L KPF+++
Sbjct: 125 YDVGDDDTKLLYKPFLNV 142
Score = 61.2 bits (147), Expect = 3e-07
Identities = 31/71 (43%), Positives = 45/71 (63%), Gaps = 1/71 (1%)
Frame = -1
Query: 686 ESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANH-KSTLQYWRRY 510
ES + L KPFV++Y GAAY+KWLS +E +ER+ +F+V+AY G AN+ + T W ++
Sbjct: 343 ESVEDLSKPFVSMYFGAAYMKWLSEYEGRERTLQFVVQAYLSGPQNANNLEETGPLWSKF 402
Query: 509 LSVKESLPSRK 477
V S K
Sbjct: 403 EEVLSRYESNK 413
>gb|EXB62639.1| hypothetical protein L484_023934 [Morus notabilis]
Length = 604
Score = 312 bits (799), Expect = 7e-83
Identities = 156/243 (64%), Positives = 186/243 (76%), Gaps = 12/243 (4%)
Frame = -1
Query: 695 EVFESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWR 516
EV +S LL++PFVNVY AAYLKWLSNF+Q+ERSEEF++RAYKGGT KA HKST+ +W+
Sbjct: 128 EVEGNSDLLFRPFVNVYFSAAYLKWLSNFDQRERSEEFVIRAYKGGTKKATHKSTMPFWQ 187
Query: 515 RYLSVKESLPSRKVFE------------VIPMPNXXXXXXXXPHTKDRPVNTTWDSRTSK 372
RYLSVKES P RK+ E V P P+ V T WDSR S
Sbjct: 188 RYLSVKESFPPRKISEDGLSLNDASSATVSPAPSVSSSQ------NSNVVYTCWDSRASA 241
Query: 371 EDMEEIWNNPIVSKEWTKSGEKKGKVRFSHDTEKRPYLSRVELKAVSEIILSKHFSTKGV 192
EDMEE+WN+ V KEW+KSGE+KGKVRFSHD KRPYLS VELKAV+EIILSKHFS+KG+
Sbjct: 242 EDMEEMWNHSEVLKEWSKSGERKGKVRFSHDEMKRPYLSLVELKAVAEIILSKHFSSKGI 301
Query: 191 KPSVLCAIAEIVSMRFVNGIGQRTGLMGIDYPTARWLYKDLGYKAYIVESVEDLTKPFVS 12
KP+VLCA+AE+VSMRFV+G+G R G+MG+DY TA WLY +LG++AY V+SVEDLTKPFVS
Sbjct: 302 KPTVLCALAEVVSMRFVHGVGPRVGIMGMDYSTAFWLYMELGFRAYKVDSVEDLTKPFVS 361
Query: 11 MCF 3
M F
Sbjct: 362 MYF 364
Score = 92.0 bits (227), Expect = 2e-16
Identities = 53/139 (38%), Positives = 83/139 (59%), Gaps = 9/139 (6%)
Frame = -1
Query: 392 WDSRTSKEDMEEIWNNPIVSKEWTKSGEKKG-KVRFSHDTEKRPYLSRVELKAVSEIILS 216
WD DME +W P V EW +GE +G KV S D + + YL++ E++AV++II+
Sbjct: 8 WDDCVDPRDMEAMWKTPEVRAEWLDAGETRGHKVHLSRDPDGQLYLTQTEMRAVADIIIC 67
Query: 215 KHFSTKGVKPSVLCAIAEIVSMR--FVNGIGQRT-----GLMGIDYPTARWLYKDLGYKA 57
+HF + + PS++CAIAE+ S R +++ G+M + TA WLY++L Y++
Sbjct: 68 RHFGPQ-LDPSMICAIAELESDRQPLATRYDKKSKDTTRGIMQLLPKTADWLYRELHYQS 126
Query: 56 YIVESVEDLT-KPFVSMCF 3
Y VE DL +PFV++ F
Sbjct: 127 YEVEGNSDLLFRPFVNVYF 145
>ref|XP_006493762.1| PREDICTED: uncharacterized protein LOC102629450 isoform X1 [Citrus
sinensis] gi|568881852|ref|XP_006493763.1| PREDICTED:
uncharacterized protein LOC102629450 isoform X2 [Citrus
sinensis] gi|568881854|ref|XP_006493764.1| PREDICTED:
uncharacterized protein LOC102629450 isoform X3 [Citrus
sinensis] gi|568881856|ref|XP_006493765.1| PREDICTED:
uncharacterized protein LOC102629450 isoform X4 [Citrus
sinensis] gi|568881858|ref|XP_006493766.1| PREDICTED:
uncharacterized protein LOC102629450 isoform X5 [Citrus
sinensis]
Length = 425
Score = 306 bits (785), Expect = 3e-81
Identities = 150/233 (64%), Positives = 182/233 (78%), Gaps = 2/233 (0%)
Frame = -1
Query: 695 EVFESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWR 516
+V ++ LL++P V++Y GAAYLKWLS F+ KER+EEF++RAYKGGT KA HKSTL YW+
Sbjct: 128 DVEQNPDLLFRPLVSIYFGAAYLKWLSTFQDKERTEEFVIRAYKGGTKKATHKSTLPYWK 187
Query: 515 RYLSVKESLPSRKVFEVIPMPNXXXXXXXXPHTKDRPVNTT--WDSRTSKEDMEEIWNNP 342
Y+SVKESLPSRK F+ P P H + T WDS T+ EDME++W +P
Sbjct: 188 SYISVKESLPSRKFFDDGPSPANASGAPPPVHAGASESSGTEYWDSITTPEDMEQMWAHP 247
Query: 341 IVSKEWTKSGEKKGKVRFSHDTEKRPYLSRVELKAVSEIILSKHFSTKGVKPSVLCAIAE 162
V KEWT+SGEK+GKVRFSHD +KR YLSRVELKAV+EIILSK+FSTKGVKP+ LCAIAE
Sbjct: 248 DVVKEWTRSGEKRGKVRFSHDAKKRSYLSRVELKAVAEIILSKYFSTKGVKPTYLCAIAE 307
Query: 161 IVSMRFVNGIGQRTGLMGIDYPTARWLYKDLGYKAYIVESVEDLTKPFVSMCF 3
+VSMRFVNG+ R GLMGIDY TA W+Y +LGY+AY V+S +DLTKPFVSM F
Sbjct: 308 MVSMRFVNGVSPRIGLMGIDYSTAFWIYMELGYRAYKVDSADDLTKPFVSMYF 360
Score = 96.3 bits (238), Expect = 8e-18
Identities = 52/139 (37%), Positives = 83/139 (59%), Gaps = 9/139 (6%)
Frame = -1
Query: 392 WDSRTSKEDMEEIWNNPIVSKEWTKSGEKKG-KVRFSHDTEKRPYLSRVELKAVSEIILS 216
WD ED++E+W VS EW +GE +G KV S D + +PYL++ E++AV+ I++
Sbjct: 8 WDDCIEAEDLKEMWKEVEVSTEWIDAGEDRGQKVHLSRDPDGQPYLTQTEMRAVAYIVVR 67
Query: 215 KHFSTKGVKPSVLCAIAEIVSMRFVNGI-------GQRTGLMGIDYPTARWLYKDLGYKA 57
+HF ++ + P ++CAIAE+ S R + + + GLM I + A WL+ ++GY+
Sbjct: 68 RHFRSQ-IDPDMICAIAELESDRQLLAVRYDKKSKEAKVGLMQITHKNAVWLFSEMGYRL 126
Query: 56 YIVESVEDLT-KPFVSMCF 3
Y VE DL +P VS+ F
Sbjct: 127 YDVEQNPDLLFRPLVSIYF 145
>ref|XP_006422271.1| hypothetical protein CICLE_v10006846mg [Citrus clementina]
gi|557524144|gb|ESR35511.1| hypothetical protein
CICLE_v10006846mg [Citrus clementina]
Length = 418
Score = 305 bits (782), Expect = 7e-81
Identities = 149/231 (64%), Positives = 180/231 (77%)
Frame = -1
Query: 695 EVFESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWR 516
+V ++ LL++P V++Y GAAYLKWLS F+ KER+EEF++RAYKGGT KA HKSTL YW+
Sbjct: 128 DVEQNPDLLFRPLVSIYFGAAYLKWLSTFQDKERTEEFVIRAYKGGTKKATHKSTLPYWK 187
Query: 515 RYLSVKESLPSRKVFEVIPMPNXXXXXXXXPHTKDRPVNTTWDSRTSKEDMEEIWNNPIV 336
Y+SVKESLPSRK F+ P P H WDS T+ EDME++W +P V
Sbjct: 188 SYISVKESLPSRKFFDDGPSPANASGAPPPVHAGTE----YWDSITTPEDMEQMWAHPDV 243
Query: 335 SKEWTKSGEKKGKVRFSHDTEKRPYLSRVELKAVSEIILSKHFSTKGVKPSVLCAIAEIV 156
KEWT+SGEK+GKVRFSHD +KR YLSRVELKAV+EIILSK+FSTKGVKP+ LCAIAE+V
Sbjct: 244 VKEWTRSGEKRGKVRFSHDAKKRSYLSRVELKAVAEIILSKYFSTKGVKPTYLCAIAEMV 303
Query: 155 SMRFVNGIGQRTGLMGIDYPTARWLYKDLGYKAYIVESVEDLTKPFVSMCF 3
SMRFVNG+ R GLMGIDY TA W+Y +LGY+AY V+S +DLTKPFVSM F
Sbjct: 304 SMRFVNGVSPRIGLMGIDYSTAFWIYMELGYRAYKVDSADDLTKPFVSMYF 354
Score = 96.3 bits (238), Expect = 8e-18
Identities = 52/139 (37%), Positives = 83/139 (59%), Gaps = 9/139 (6%)
Frame = -1
Query: 392 WDSRTSKEDMEEIWNNPIVSKEWTKSGEKKG-KVRFSHDTEKRPYLSRVELKAVSEIILS 216
WD ED++E+W VS EW +GE +G KV S D + +PYL++ E++AV+ I++
Sbjct: 8 WDDCIEAEDLKEMWKEVEVSTEWIDAGEDRGQKVHLSRDPDGQPYLTQTEMRAVAYIVVR 67
Query: 215 KHFSTKGVKPSVLCAIAEIVSMRFVNGI-------GQRTGLMGIDYPTARWLYKDLGYKA 57
+HF ++ + P ++CAIAE+ S R + + + GLM I + A WL+ ++GY+
Sbjct: 68 RHFRSQ-IDPDMICAIAELESDRQLLAVRYDKKSKEAKVGLMQITHKNAVWLFSEMGYRL 126
Query: 56 YIVESVEDLT-KPFVSMCF 3
Y VE DL +P VS+ F
Sbjct: 127 YDVEQNPDLLFRPLVSIYF 145
>ref|XP_002316147.1| hypothetical protein POPTR_0010s17920g [Populus trichocarpa]
gi|222865187|gb|EEF02318.1| hypothetical protein
POPTR_0010s17920g [Populus trichocarpa]
Length = 455
Score = 300 bits (769), Expect = 2e-79
Identities = 152/263 (57%), Positives = 188/263 (71%), Gaps = 32/263 (12%)
Frame = -1
Query: 695 EVFESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWR 516
EV + +LY+PFV+VY GAAY+KWLSNFE++ERSEEF+VRAY GGT KA HKSTLQYW+
Sbjct: 129 EVEGNPDILYRPFVSVYFGAAYIKWLSNFEEEERSEEFVVRAYNGGTKKATHKSTLQYWK 188
Query: 515 RYLSVKESLPSRKVFEVIPMPNXXXXXXXXP----------------HTKDRPVNTT--- 393
RYLSVKESLPSR+ + P N ++++ P T
Sbjct: 189 RYLSVKESLPSRRFVDEGPSVNNAHSSTAQAAPATQNTNAPSSEKTGYSRNCPFRTFRGD 248
Query: 392 -------------WDSRTSKEDMEEIWNNPIVSKEWTKSGEKKGKVRFSHDTEKRPYLSR 252
WDS+ S E+M+E+W+ V+KEWTKSGEK+GKVRFSHD + +PYLSR
Sbjct: 249 YASVKEAGVDYIYWDSKASPEEMQEMWSRSEVAKEWTKSGEKRGKVRFSHDKDMKPYLSR 308
Query: 251 VELKAVSEIILSKHFSTKGVKPSVLCAIAEIVSMRFVNGIGQRTGLMGIDYPTARWLYKD 72
VE+KAV++IILSKHFST+GVKPSV+CA+AE+VSMRFVNG+G R GLMGIDY TA WLY +
Sbjct: 309 VEMKAVADIILSKHFSTRGVKPSVICALAEMVSMRFVNGVGPRIGLMGIDYSTAFWLYME 368
Query: 71 LGYKAYIVESVEDLTKPFVSMCF 3
LGY+AY ++S DLTKPFVSM F
Sbjct: 369 LGYRAYRLDSAGDLTKPFVSMYF 391
Score = 104 bits (259), Expect = 3e-20
Identities = 59/140 (42%), Positives = 88/140 (62%), Gaps = 10/140 (7%)
Frame = -1
Query: 392 WDSRTSKEDMEEIWNNPIVSKEWTKSGEKKG-KVRFSHDTEKRPYLSRVELKAVSEIILS 216
W+ +DME +W P VS EW +GE KG KV S D + PYL++ E+KAV++II+
Sbjct: 8 WNDCVDVQDMEAMWQEPEVSTEWLDAGETKGNKVHLSRDPDGEPYLTQTEMKAVADIIVR 67
Query: 215 KHFSTKGVKPSVLCAIAEI------VSMRFVNGIGQRT--GLMGIDYPTARWLYKDLGYK 60
+HF ++ ++P ++CA+AE+ +S R+ + + T G+M I TA WL +DLGY+
Sbjct: 68 RHFDSQ-IQPDMICAVAELASDRQPLSTRWYDKKTKETALGIMQILPKTAEWLVRDLGYQ 126
Query: 59 AYIVESVED-LTKPFVSMCF 3
AY VE D L +PFVS+ F
Sbjct: 127 AYEVEGNPDILYRPFVSVYF 146
Score = 61.2 bits (147), Expect = 3e-07
Identities = 27/59 (45%), Positives = 39/59 (66%)
Frame = -1
Query: 686 ESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWRRY 510
+S+ L KPFV++Y GAAYL WLS +E +ER+ +F+V AY G NH+ T W ++
Sbjct: 377 DSAGDLTKPFVSMYFGAAYLAWLSEYEGRERTPQFVVPAYLSGPKNVNHQETGPLWLKF 435
>gb|EOY14197.1| Vacuole, catalytic [Theobroma cacao]
Length = 426
Score = 299 bits (766), Expect = 5e-79
Identities = 148/235 (62%), Positives = 184/235 (78%), Gaps = 5/235 (2%)
Frame = -1
Query: 692 VFESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWRR 513
V E +LY+PFVNVY GAAYL+WLS F+ ++R+EEF+VRAY+GGT KA+HKSTL YW+R
Sbjct: 129 VEEDPNILYRPFVNVYFGAAYLQWLSKFDDEDRTEEFIVRAYRGGTKKASHKSTLPYWKR 188
Query: 512 YLSVKESLPSRKVFEVIPMPNXXXXXXXXPHTKDRP-----VNTTWDSRTSKEDMEEIWN 348
YLSVKES SRK F+ P P P + P V WD R S EDME++WN
Sbjct: 189 YLSVKESFLSRKHFDG-PSPTQAPTSPTSPVSPVSPCSASGVGLNWDVRVSSEDMEQMWN 247
Query: 347 NPIVSKEWTKSGEKKGKVRFSHDTEKRPYLSRVELKAVSEIILSKHFSTKGVKPSVLCAI 168
+P VSKEWTKS E++GKVRFS+D EK+PYLSR+ELKA++EII+SK+FST+G+KP+VLCA+
Sbjct: 248 HPDVSKEWTKSKERRGKVRFSYDKEKKPYLSRIELKAIAEIIVSKYFSTRGIKPTVLCAL 307
Query: 167 AEIVSMRFVNGIGQRTGLMGIDYPTARWLYKDLGYKAYIVESVEDLTKPFVSMCF 3
AE+VSMRFV+GI R G+MGIDY TA WLY ++GY+AY V+ VEDLTKPFVSM F
Sbjct: 308 AEMVSMRFVDGIEARAGIMGIDYSTAFWLYTEVGYRAYRVDYVEDLTKPFVSMYF 362
Score = 85.9 bits (211), Expect = 1e-14
Identities = 49/139 (35%), Positives = 82/139 (58%), Gaps = 9/139 (6%)
Frame = -1
Query: 392 WDSRTSKEDMEEIWNNPIVSKEWTKSGEKKG-KVRFSHDTEKRPYLSRVELKAVSEIILS 216
WD ++MEE+W P V EW +GE +G +V S D E + YL++ E++AV+EI+
Sbjct: 8 WDDCIDPQNMEEMWKQPEVRTEWIDAGETQGQRVHLSRDPEGQSYLTQTEMRAVAEIVTR 67
Query: 215 KHFSTKGVKPSVLCAIAEI------VSMRFVNGIGQRT-GLMGIDYPTARWLYKDLGYKA 57
HF ++ + P ++CAIA++ +S+R+ T G+M I TA W++++ Y +
Sbjct: 68 IHFHSQ-IDPEMICAIADLESDRRPLSIRYDKKSKVTTIGIMQIAPKTAEWMFRECDYSS 126
Query: 56 YIVESVED-LTKPFVSMCF 3
Y VE + L +PFV++ F
Sbjct: 127 YPVEEDPNILYRPFVNVYF 145
Score = 60.1 bits (144), Expect = 7e-07
Identities = 28/65 (43%), Positives = 39/65 (60%)
Frame = -1
Query: 671 LYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWRRYLSVKES 492
L KPFV++Y GAAY WLS +E +ER+ +F+V+AY G N + T W ++ V S
Sbjct: 353 LTKPFVSMYFGAAYFAWLSEYEGRERTPQFVVQAYLAGPKNVNLQETGPMWLKFEQVLSS 412
Query: 491 LPSRK 477
RK
Sbjct: 413 YEPRK 417
>ref|XP_002311290.2| hypothetical protein POPTR_0008s08370g [Populus trichocarpa]
gi|550332662|gb|EEE88657.2| hypothetical protein
POPTR_0008s08370g [Populus trichocarpa]
Length = 395
Score = 290 bits (742), Expect = 3e-76
Identities = 145/231 (62%), Positives = 174/231 (75%)
Frame = -1
Query: 695 EVFESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWR 516
EV + ++L +PFV+VY GAAYLKWLSNFE+K RSEEF+VRAY GG KA HKSTL YW+
Sbjct: 129 EVEGNPEMLCRPFVSVYFGAAYLKWLSNFEKKVRSEEFIVRAYDGGPKKATHKSTLPYWK 188
Query: 515 RYLSVKESLPSRKVFEVIPMPNXXXXXXXXPHTKDRPVNTTWDSRTSKEDMEEIWNNPIV 336
RYL VKES V WDS+ S EDM+E+W++ V
Sbjct: 189 RYLCVKESF----------------------------VYIYWDSKASLEDMQEMWSHSEV 220
Query: 335 SKEWTKSGEKKGKVRFSHDTEKRPYLSRVELKAVSEIILSKHFSTKGVKPSVLCAIAEIV 156
++EWT SGEK+GKVRFSHD KRPYLSRVE+KAV++IILSKHFST+GVKPSVLCA+AE+V
Sbjct: 221 AEEWTNSGEKRGKVRFSHDQNKRPYLSRVEMKAVADIILSKHFSTRGVKPSVLCALAEMV 280
Query: 155 SMRFVNGIGQRTGLMGIDYPTARWLYKDLGYKAYIVESVEDLTKPFVSMCF 3
SMRFVNG+G RTGLMGIDY TA WLY +LGY+AY V+SV+D+TKPFVS+ F
Sbjct: 281 SMRFVNGVGPRTGLMGIDYSTAFWLYMELGYRAYRVDSVDDMTKPFVSVYF 331
Score = 104 bits (260), Expect = 2e-20
Identities = 61/140 (43%), Positives = 88/140 (62%), Gaps = 10/140 (7%)
Frame = -1
Query: 392 WDSRTSKEDMEEIWNNPIVSKEWTKSGEKKG-KVRFSHDTEKRPYLSRVELKAVSEIILS 216
W+ +DME +W P VS EW +GE KG KV S D + PYL++ E+KAV++II+
Sbjct: 8 WNDCVDVQDMEAMWREPEVSTEWLDAGETKGNKVHLSRDPDGEPYLTQTEMKAVADIIVR 67
Query: 215 KHFSTKGVKPSVLCAIAEI------VSMRFVNGIGQRT--GLMGIDYPTARWLYKDLGYK 60
+HF ++ V P ++CAIAE+ +S R+ + + T G+M I TA WL +DLGY+
Sbjct: 68 RHFDSQ-VHPDMICAIAELASDRQPLSTRWYDKKTKETALGIMQILPKTAEWLVRDLGYR 126
Query: 59 AYIVE-SVEDLTKPFVSMCF 3
AY VE + E L +PFVS+ F
Sbjct: 127 AYEVEGNPEMLCRPFVSVYF 146
Score = 56.6 bits (135), Expect = 7e-06
Identities = 24/52 (46%), Positives = 34/52 (65%)
Frame = -1
Query: 665 KPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWRRY 510
KPFV+VY GAAYL WLS +E +ER+ +F+V AY G N + + W ++
Sbjct: 324 KPFVSVYFGAAYLAWLSEYEGRERTPQFVVPAYLSGPKNVNRQESGPLWLKF 375
>ref|XP_004308235.1| PREDICTED: uncharacterized protein LOC101294021 [Fragaria vesca
subsp. vesca]
Length = 444
Score = 290 bits (741), Expect = 4e-76
Identities = 144/243 (59%), Positives = 182/243 (74%), Gaps = 16/243 (6%)
Frame = -1
Query: 683 SSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWRRYLS 504
+ LL++PF N+Y GAAYL WLS+F+QKERSEEF+VRAYKGGT KA HKSTL+YW+RYLS
Sbjct: 132 NDSLLFRPFENIYFGAAYLIWLSSFDQKERSEEFVVRAYKGGTKKATHKSTLKYWQRYLS 191
Query: 503 VKESLPSRKVFE------------VIPMPNXXXXXXXXPHTKDRPVN----TTWDSRTSK 372
VKESLPSRK E + + K R ++ T WDS S
Sbjct: 192 VKESLPSRKSLEGRKSVEEVSASLALAKKSASSQNTVLLTPKPRKLSGGTYTYWDSVASP 251
Query: 371 EDMEEIWNNPIVSKEWTKSGEKKGKVRFSHDTEKRPYLSRVELKAVSEIILSKHFSTKGV 192
EDME++WN+P+VSKEWT +GEK+G+VRFS D +KRPYLSR+ELKAV+EIIL KHFS K +
Sbjct: 252 EDMEDMWNHPVVSKEWTLTGEKQGQVRFSQDDKKRPYLSRMELKAVAEIILIKHFSAKPL 311
Query: 191 KPSVLCAIAEIVSMRFVNGIGQRTGLMGIDYPTARWLYKDLGYKAYIVESVEDLTKPFVS 12
K SVLCA+AEI+SMRF++GIG R G+MGI+Y TA WLY+++G++AY + S +DL+KPFVS
Sbjct: 312 KVSVLCALAEIISMRFLSGIGPRPGIMGIEYSTAVWLYREMGFRAYRISSADDLSKPFVS 371
Query: 11 MCF 3
M F
Sbjct: 372 MYF 374
Score = 84.7 bits (208), Expect = 2e-14
Identities = 54/141 (38%), Positives = 80/141 (56%), Gaps = 11/141 (7%)
Frame = -1
Query: 392 WDSRTSKEDMEEIWNNPIVSKEWTKSGEKKG-KVRFSHDTEKRPYLSRVELKAVSEIILS 216
W+ DM+ +WN P VS EW +GE KG KV S D + + YL++ E+KAV+EII+
Sbjct: 8 WNECIDPLDMQALWNVPNVSAEWEDAGETKGQKVHLSRDPDGQVYLTQTEMKAVAEIIVR 67
Query: 215 KHFSTKGVKPSVLCAIAEIVSMRFVNGIGQR---------TGLMGIDYPTARWLYKDLGY 63
+HF+ K + ++CAIAE+ S R + QR G+M I TA WL + GY
Sbjct: 68 RHFTGK-IDAEMICAIAELESDR--QPLAQRYDKKAKATHLGIMQILPKTADWLASEKGY 124
Query: 62 KAYIVESVEDLT-KPFVSMCF 3
AY ++ + L +PF ++ F
Sbjct: 125 GAYQLKGNDSLLFRPFENIYF 145
>ref|XP_003532919.1| PREDICTED: uncharacterized protein LOC100798065 [Glycine max]
Length = 423
Score = 287 bits (734), Expect = 3e-75
Identities = 139/232 (59%), Positives = 178/232 (76%), Gaps = 1/232 (0%)
Frame = -1
Query: 695 EVFESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWR 516
E E+ + L+KPFVNVY GAAY+KWLSNFE K+RSEEF+VRAYKGGT KA HKSTL+YW
Sbjct: 128 EADENREFLFKPFVNVYFGAAYIKWLSNFENKKRSEEFIVRAYKGGTKKATHKSTLRYWN 187
Query: 515 RYLSVKESLPSRK-VFEVIPMPNXXXXXXXXPHTKDRPVNTTWDSRTSKEDMEEIWNNPI 339
YLSVKES SRK V + + P ++KD V+T WDSR + EDME +WN+
Sbjct: 188 CYLSVKESFRSRKSVDDNVSPPAHSHPLPSLENSKDASVDTYWDSRVAPEDMEAMWNHLE 247
Query: 338 VSKEWTKSGEKKGKVRFSHDTEKRPYLSRVELKAVSEIILSKHFSTKGVKPSVLCAIAEI 159
V KEW KS +K+GKVRF+HD +KRPYLSRVE+KA+++IIL K+ ST +K +V+CAI E+
Sbjct: 248 VRKEWNKSKQKQGKVRFAHDEKKRPYLSRVEMKAIADIILYKYLSTVKIKSTVICAIGEV 307
Query: 158 VSMRFVNGIGQRTGLMGIDYPTARWLYKDLGYKAYIVESVEDLTKPFVSMCF 3
+S RF++G+G++ G+MGIDY TA WLY +LGY+AY +ESV+DL PFVSM F
Sbjct: 308 LSKRFLHGVGEQPGIMGIDYSTAYWLYLELGYRAYRLESVDDLNNPFVSMYF 359
Score = 96.7 bits (239), Expect = 6e-18
Identities = 55/139 (39%), Positives = 85/139 (61%), Gaps = 9/139 (6%)
Frame = -1
Query: 392 WDSRTSKEDMEEIWNNPIVSKEWTKSGEKK-GKVRFSHDTEKRPYLSRVELKAVSEIILS 216
WD ++ E +WN P VS EW K+GE++ KV S D + +PYL++ E++AV++II++
Sbjct: 8 WDDCVDPQNFEAMWNVPEVSAEWIKAGEQRCQKVHLSRDPDGQPYLTQTEMRAVADIIIT 67
Query: 215 KHFSTKGVKPSVLCAIAEIVSMRFVNGIGQR-------TGLMGIDYPTARWLYKDLGYKA 57
KHF ++ + P ++CAIAE+ S R + + R GLM + T WL +LGY +
Sbjct: 68 KHFHSE-IDPDMICAIAELESDRQLLVMNSRHKSKEPTVGLMQLLPKTTEWLMSELGYCS 126
Query: 56 YIV-ESVEDLTKPFVSMCF 3
Y E+ E L KPFV++ F
Sbjct: 127 YEADENREFLFKPFVNVYF 145
>gb|EMJ13701.1| hypothetical protein PRUPE_ppa017705mg [Prunus persica]
Length = 438
Score = 284 bits (726), Expect = 2e-74
Identities = 142/240 (59%), Positives = 178/240 (74%), Gaps = 16/240 (6%)
Frame = -1
Query: 674 LLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWRRYLSVKE 495
LL+KPFVNVY GAAYLKWLS+++Q ERSEE++VRAYKGGT KA HKSTL+YW+RYLSVKE
Sbjct: 135 LLFKPFVNVYFGAAYLKWLSSYDQTERSEEYVVRAYKGGTKKATHKSTLKYWQRYLSVKE 194
Query: 494 SLPSRKVFEVIPMPNXXXXXXXXP---------HTKDRPV-------NTTWDSRTSKEDM 363
SLPS FE P PN H P + WDSR S EDM
Sbjct: 195 SLPSS--FEGRPSPNEVSTSAAPHAHAPPHAPPHAHAAPPAPHNAGHHIYWDSRASPEDM 252
Query: 362 EEIWNNPIVSKEWTKSGEKKGKVRFSHDTEKRPYLSRVELKAVSEIILSKHFSTKGVKPS 183
E+WN+ V++EWT +GEK+GKVRFS D +KRPYLSRVELKAV++I+L+KHF+TK +K S
Sbjct: 253 AEMWNHQDVAREWTTTGEKRGKVRFSLDDKKRPYLSRVELKAVADIVLTKHFNTKPIKAS 312
Query: 182 VLCAIAEIVSMRFVNGIGQRTGLMGIDYPTARWLYKDLGYKAYIVESVEDLTKPFVSMCF 3
VL A+AE++S+RF+NG+G RTG+MGIDY TA WL+ ++G+KAY + S +DL+ PFVSM F
Sbjct: 313 VLGALAEVISLRFLNGVGPRTGIMGIDYSTALWLFMEMGFKAYTIHSADDLSMPFVSMYF 372
Score = 90.9 bits (224), Expect = 3e-16
Identities = 50/139 (35%), Positives = 83/139 (59%), Gaps = 9/139 (6%)
Frame = -1
Query: 392 WDSRTSKEDMEEIWNNPIVSKEWTKSGEKKG-KVRFSHDTEKRPYLSRVELKAVSEIILS 216
W+ D+EE+W++P V+ EW +GE +G KV S D + +P+L++ E+K V+EI +
Sbjct: 8 WEDCVDSHDLEEMWSSPCVTAEWLDAGESRGQKVHLSRDPDGQPFLTQTEMKIVAEITIR 67
Query: 215 KHFSTKGVKPSVLCAIAEIVSMR--FVNGIGQRT-----GLMGIDYPTARWLYKDLGYKA 57
+HF+ + + P ++ AIA++ S R ++T G+M + TA WL D+GY+
Sbjct: 68 RHFANQ-IDPDMIRAIADLESDRQPLATRYDKKTKSTTAGIMQLLPKTADWLASDMGYQV 126
Query: 56 YIVESVEDLT-KPFVSMCF 3
Y V+ DL KPFV++ F
Sbjct: 127 YAVQGNPDLLFKPFVNVYF 145
>gb|AAD34680.1|AC006341_8 Contains a PF|01464 Transglycosylase SLT domain [Arabidopsis
thaliana]
Length = 455
Score = 284 bits (726), Expect = 2e-74
Identities = 138/230 (60%), Positives = 173/230 (75%)
Frame = -1
Query: 692 VFESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWRR 513
V ++ LL+KPF+NVY GAAYLKWL++++ +RSEEF+VRAY GGT KA HKSTL YW+R
Sbjct: 129 VDDNPDLLHKPFINVYFGAAYLKWLTDYQNNQRSEEFVVRAYNGGTKKATHKSTLPYWKR 188
Query: 512 YLSVKESLPSRKVFEVIPMPNXXXXXXXXPHTKDRPVNTTWDSRTSKEDMEEIWNNPIVS 333
YL+VKESLPSRK + P D T WDSR S EDME++WN +
Sbjct: 189 YLAVKESLPSRKHGDAGPSSFRPTNPASPGSNTDF---TYWDSRASPEDMEDMWNQSEIC 245
Query: 332 KEWTKSGEKKGKVRFSHDTEKRPYLSRVELKAVSEIILSKHFSTKGVKPSVLCAIAEIVS 153
KEWTKS E++GKVRFS D EKRPYLSR ELKAV+EII+SK+FSTKG++ ++CAIA+ V
Sbjct: 246 KEWTKSKEERGKVRFSQDGEKRPYLSRGELKAVAEIIVSKYFSTKGIRVPLVCAIADTVC 305
Query: 152 MRFVNGIGQRTGLMGIDYPTARWLYKDLGYKAYIVESVEDLTKPFVSMCF 3
MRFVNGI + G++G+DY TA WLY +LGY+AY V+S +DLTKPF+SM F
Sbjct: 306 MRFVNGIKKHVGILGVDYSTASWLYSELGYRAYRVDSADDLTKPFISMYF 355
Score = 91.7 bits (226), Expect = 2e-16
Identities = 54/141 (38%), Positives = 83/141 (58%), Gaps = 9/141 (6%)
Frame = -1
Query: 398 TTWDSRTSKEDMEEIWNNPIVSKEWTKSGEKKG-KVRFSHDTEKRPYLSRVELKAVSEII 222
T W+ ED+EE+W +P VS EW GE KG KV S D + +PYL++ E++AVS+I
Sbjct: 6 TFWNDCVEPEDLEEMWMDPAVSAEWIDVGETKGQKVHLSRDPDGQPYLTQTEMRAVSDIT 65
Query: 221 LSKHFSTKGVKPSVLCAIAEIVSMR--FVNGIGQRT-----GLMGIDYPTARWLYKDLGY 63
+ +HF + + ++CAIAE+ S R + ++T G++ + TA WL GY
Sbjct: 66 VRRHFDSI-LDSEMICAIAELESDRKPLIMRYSKKTKETGLGILQVFEKTAAWLAGGQGY 124
Query: 62 KAYIVESVEDLT-KPFVSMCF 3
+AY V+ DL KPF+++ F
Sbjct: 125 QAYNVDDNPDLLHKPFINVYF 145
>ref|NP_173079.2| uncharacterized protein [Arabidopsis thaliana]
gi|119935884|gb|ABM06023.1| At1g16290 [Arabidopsis
thaliana] gi|332191311|gb|AEE29432.1| uncharacterized
protein AT1G16290 [Arabidopsis thaliana]
Length = 419
Score = 284 bits (726), Expect = 2e-74
Identities = 138/230 (60%), Positives = 173/230 (75%)
Frame = -1
Query: 692 VFESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWRR 513
V ++ LL+KPF+NVY GAAYLKWL++++ +RSEEF+VRAY GGT KA HKSTL YW+R
Sbjct: 129 VDDNPDLLHKPFINVYFGAAYLKWLTDYQNNQRSEEFVVRAYNGGTKKATHKSTLPYWKR 188
Query: 512 YLSVKESLPSRKVFEVIPMPNXXXXXXXXPHTKDRPVNTTWDSRTSKEDMEEIWNNPIVS 333
YL+VKESLPSRK + P D T WDSR S EDME++WN +
Sbjct: 189 YLAVKESLPSRKHGDAGPSSFRPTNPASPGSNTDF---TYWDSRASPEDMEDMWNQSEIC 245
Query: 332 KEWTKSGEKKGKVRFSHDTEKRPYLSRVELKAVSEIILSKHFSTKGVKPSVLCAIAEIVS 153
KEWTKS E++GKVRFS D EKRPYLSR ELKAV+EII+SK+FSTKG++ ++CAIA+ V
Sbjct: 246 KEWTKSKEERGKVRFSQDGEKRPYLSRGELKAVAEIIVSKYFSTKGIRVPLVCAIADTVC 305
Query: 152 MRFVNGIGQRTGLMGIDYPTARWLYKDLGYKAYIVESVEDLTKPFVSMCF 3
MRFVNGI + G++G+DY TA WLY +LGY+AY V+S +DLTKPF+SM F
Sbjct: 306 MRFVNGIKKHVGILGVDYSTASWLYSELGYRAYRVDSADDLTKPFISMYF 355
Score = 91.7 bits (226), Expect = 2e-16
Identities = 54/141 (38%), Positives = 83/141 (58%), Gaps = 9/141 (6%)
Frame = -1
Query: 398 TTWDSRTSKEDMEEIWNNPIVSKEWTKSGEKKG-KVRFSHDTEKRPYLSRVELKAVSEII 222
T W+ ED+EE+W +P VS EW GE KG KV S D + +PYL++ E++AVS+I
Sbjct: 6 TFWNDCVEPEDLEEMWMDPAVSAEWIDVGETKGQKVHLSRDPDGQPYLTQTEMRAVSDIT 65
Query: 221 LSKHFSTKGVKPSVLCAIAEIVSMR--FVNGIGQRT-----GLMGIDYPTARWLYKDLGY 63
+ +HF + + ++CAIAE+ S R + ++T G++ + TA WL GY
Sbjct: 66 VRRHFDSI-LDSEMICAIAELESDRKPLIMRYSKKTKETGLGILQVFEKTAAWLAGGQGY 124
Query: 62 KAYIVESVEDLT-KPFVSMCF 3
+AY V+ DL KPF+++ F
Sbjct: 125 QAYNVDDNPDLLHKPFINVYF 145
>ref|XP_006307632.1| hypothetical protein CARUB_v10009260mg [Capsella rubella]
gi|482576343|gb|EOA40530.1| hypothetical protein
CARUB_v10009260mg [Capsella rubella]
Length = 419
Score = 283 bits (725), Expect = 3e-74
Identities = 139/230 (60%), Positives = 172/230 (74%)
Frame = -1
Query: 692 VFESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWRR 513
V ++ LL+KPF+NVY GAAYLKWL++++ +RSEEF+VRAY GGT KA HKSTL YW+R
Sbjct: 129 VDDNPDLLHKPFINVYFGAAYLKWLTDYQNNQRSEEFVVRAYNGGTKKATHKSTLPYWKR 188
Query: 512 YLSVKESLPSRKVFEVIPMPNXXXXXXXXPHTKDRPVNTTWDSRTSKEDMEEIWNNPIVS 333
YLSVKESLPSRK + P + T WDSR S EDME++WN+ V
Sbjct: 189 YLSVKESLPSRKHVDAGPSSFHPTNPASPGSNTNF---TYWDSRVSPEDMEDMWNHSEVC 245
Query: 332 KEWTKSGEKKGKVRFSHDTEKRPYLSRVELKAVSEIILSKHFSTKGVKPSVLCAIAEIVS 153
KEWTKS E+KGKVRFS D EKRPYLSR ELKAV EII+SK+FSTKG++ ++CA+A+ V
Sbjct: 246 KEWTKSKEEKGKVRFSQDGEKRPYLSRGELKAVGEIIVSKYFSTKGIRVPLVCAVADTVC 305
Query: 152 MRFVNGIGQRTGLMGIDYPTARWLYKDLGYKAYIVESVEDLTKPFVSMCF 3
MRFVNG + G++G+DY TA WLY +LGY+AY V+S +DLTKPFVSM F
Sbjct: 306 MRFVNGTKKHVGILGVDYSTASWLYSELGYRAYRVDSADDLTKPFVSMYF 355
Score = 95.9 bits (237), Expect = 1e-17
Identities = 56/141 (39%), Positives = 84/141 (59%), Gaps = 9/141 (6%)
Frame = -1
Query: 398 TTWDSRTSKEDMEEIWNNPIVSKEWTKSGEKKG-KVRFSHDTEKRPYLSRVELKAVSEII 222
T W+ ED+EE+W +P VS EW GE KG KV S D + +PYL++ E+KAV++II
Sbjct: 6 TFWNDCVEPEDLEEMWMDPAVSAEWIDVGETKGQKVHLSRDPDGQPYLTQTEMKAVADII 65
Query: 221 LSKHFSTKGVKPSVLCAIAEIVSMR--FVNGIGQRT-----GLMGIDYPTARWLYKDLGY 63
+ +HF + P ++CAIAE+ S R + ++T G++ + TA WL GY
Sbjct: 66 VRRHFDLI-LDPEMICAIAELESDRKPLIMRYNKKTKETGLGILQVFEKTAAWLAGGQGY 124
Query: 62 KAYIVESVEDLT-KPFVSMCF 3
+AY V+ DL KPF+++ F
Sbjct: 125 QAYNVDDNPDLLHKPFINVYF 145
>ref|XP_002892901.1| hypothetical protein ARALYDRAFT_312606 [Arabidopsis lyrata subsp.
lyrata] gi|297338743|gb|EFH69160.1| hypothetical protein
ARALYDRAFT_312606 [Arabidopsis lyrata subsp. lyrata]
Length = 458
Score = 282 bits (721), Expect = 8e-74
Identities = 138/230 (60%), Positives = 173/230 (75%)
Frame = -1
Query: 692 VFESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWRR 513
V ++ LL+KPF+NVY GAAYLKWL++++ +RSEEF+VRAY GGT KA HKSTL YW+R
Sbjct: 129 VDDNPDLLHKPFINVYFGAAYLKWLTDYQNNQRSEEFVVRAYNGGTKKATHKSTLPYWKR 188
Query: 512 YLSVKESLPSRKVFEVIPMPNXXXXXXXXPHTKDRPVNTTWDSRTSKEDMEEIWNNPIVS 333
YL+VKESLPSRK + P + T WDSR S EDME++WN+ V
Sbjct: 189 YLAVKESLPSRKHVDAGPSSFHPTNPASPGSNTNF---TYWDSRASPEDMEDMWNHSEVC 245
Query: 332 KEWTKSGEKKGKVRFSHDTEKRPYLSRVELKAVSEIILSKHFSTKGVKPSVLCAIAEIVS 153
KEWTKS E++GKVRFS D EKRPYLSR ELKAV+EII+SK+FSTKG++ ++CAIA+ V
Sbjct: 246 KEWTKSKEERGKVRFSQDGEKRPYLSRGELKAVAEIIVSKYFSTKGIRVPLVCAIADTVC 305
Query: 152 MRFVNGIGQRTGLMGIDYPTARWLYKDLGYKAYIVESVEDLTKPFVSMCF 3
MRFVNG + G++G+DY TA WLY +LGY+AY V+S +DLTKPFVSM F
Sbjct: 306 MRFVNGTKKHVGILGVDYSTASWLYSELGYRAYRVDSADDLTKPFVSMYF 355
Score = 89.7 bits (221), Expect = 8e-16
Identities = 53/141 (37%), Positives = 83/141 (58%), Gaps = 9/141 (6%)
Frame = -1
Query: 398 TTWDSRTSKEDMEEIWNNPIVSKEWTKSGEKKG-KVRFSHDTEKRPYLSRVELKAVSEII 222
T W+ ED+EE+W +P VS EW GE KG KV S D + +PYL++ E++AV++I
Sbjct: 6 TFWNDCVEPEDLEEMWMDPAVSAEWIDVGETKGQKVHLSRDPDGQPYLTQTEMRAVADIT 65
Query: 221 LSKHFSTKGVKPSVLCAIAEIVSMR--FVNGIGQRT-----GLMGIDYPTARWLYKDLGY 63
+ +HF + + ++CAIAE+ S R + ++T G++ + TA WL GY
Sbjct: 66 VRRHFDSI-LDLEMICAIAELESDRKPLIMRYNKKTKETGLGILQVFEKTAAWLAGGQGY 124
Query: 62 KAYIVESVEDLT-KPFVSMCF 3
+AY V+ DL KPF+++ F
Sbjct: 125 QAYNVDDNPDLLHKPFINVYF 145
Score = 56.2 bits (134), Expect = 9e-06
Identities = 24/59 (40%), Positives = 39/59 (66%)
Frame = -1
Query: 686 ESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWRRY 510
+S+ L KPFV++Y G AYL WLS +E +RS +F+V+AY G D + +++ W ++
Sbjct: 341 DSADDLTKPFVSMYFGVAYLVWLSEYEGSQRSNQFIVQAYINGPDHVDLEASCPLWLKF 399
>ref|NP_001046030.1| Os02g0170900 [Oryza sativa Japonica Group]
gi|49387564|dbj|BAD25495.1| unknown protein [Oryza
sativa Japonica Group] gi|49388081|dbj|BAD25193.1|
unknown protein [Oryza sativa Japonica Group]
gi|113535561|dbj|BAF07944.1| Os02g0170900 [Oryza sativa
Japonica Group] gi|215767047|dbj|BAG99275.1| unnamed
protein product [Oryza sativa Japonica Group]
Length = 419
Score = 258 bits (660), Expect = 1e-66
Identities = 129/231 (55%), Positives = 164/231 (70%)
Frame = -1
Query: 695 EVFESSKLLYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWR 516
++ E + LLY PFVNVY GAAY KWL + + KER+EEF+VRAYKGG KA HKST ++
Sbjct: 128 DIEEDANLLYWPFVNVYFGAAYAKWLFSCDDKERTEEFVVRAYKGGKKKAVHKSTSPIFQ 187
Query: 515 RYLSVKESLPSRKVFEVIPMPNXXXXXXXXPHTKDRPVNTTWDSRTSKEDMEEIWNNPIV 336
RYL VKE+L S + E N + WDS+ S+ DM+ +W+ P V
Sbjct: 188 RYLYVKEALLSMRQPESF---NDLTPNLLANSSSTEGQLIYWDSKVSEVDMDAMWSQPDV 244
Query: 335 SKEWTKSGEKKGKVRFSHDTEKRPYLSRVELKAVSEIILSKHFSTKGVKPSVLCAIAEIV 156
KEWTKSGE++G VRFSHD+++RPYLSRVE+KAV+EI +S+H S+KGV P L A+AE+
Sbjct: 245 IKEWTKSGERRGNVRFSHDSKRRPYLSRVEVKAVAEITISRHLSSKGVTPEALAALAEVC 304
Query: 155 SMRFVNGIGQRTGLMGIDYPTARWLYKDLGYKAYIVESVEDLTKPFVSMCF 3
SMRFV+G+ RTGLMGIDYPTA WLY+D G++AY V SV+DL PF SM F
Sbjct: 305 SMRFVHGVRSRTGLMGIDYPTAAWLYRDCGHRAYTVSSVDDLYNPFTSMYF 355
Score = 89.0 bits (219), Expect = 1e-15
Identities = 54/139 (38%), Positives = 82/139 (58%), Gaps = 9/139 (6%)
Frame = -1
Query: 392 WDSRTSKEDMEEIWNNPIVSKEWTKSGEKKG-KVRFSHDTEKRPYLSRVELKAVSEIILS 216
WD EDM+ +W +P VSKEW +GE G KV S D + YL++ E+ AV+ I +
Sbjct: 8 WDDCLDPEDMQLMWADPHVSKEWIDAGEGPGQKVHLSRDPDGEAYLTQTEMMAVAAITVH 67
Query: 215 KHFSTKGVKPSVLCAIAEIVSMR--FVNGIGQRT-----GLMGIDYPTARWLYKDLGYKA 57
KHF ++ + P ++ A+AEI S R FV+ ++T G+M + A+WL ++LGYK
Sbjct: 68 KHFKSQ-LDPYMIGALAEIASGRRLFVDNYDRKTKEIKAGIMQVTIEVAQWLGRELGYKY 126
Query: 56 YIVESVEDLTK-PFVSMCF 3
Y +E +L PFV++ F
Sbjct: 127 YDIEEDANLLYWPFVNVYF 145
Score = 65.5 bits (158), Expect = 2e-08
Identities = 27/55 (49%), Positives = 39/55 (70%)
Frame = -1
Query: 671 LYKPFVNVYLGAAYLKWLSNFEQKERSEEFMVRAYKGGTDKANHKSTLQYWRRYL 507
LY PF ++Y GAAYL WLS +E +ERS EF+V+AY GG + + + T +W ++L
Sbjct: 346 LYNPFTSMYFGAAYLGWLSQYEGRERSHEFIVQAYLGGPENVSLQETGPFWNKFL 400