BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00037130
         (619 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima]             299   3e-79
ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chlorop...   279   3e-73
emb|CBI24150.3| unnamed protein product [Vitis vinifera]              279   3e-73
gb|AAD34295.1|AF097311_1 kaurene synthase [Stevia rebaudiana]         256   2e-66
gb|AAD34294.1|AF097310_1 kaurene synthase [Stevia rebaudiana]         252   4e-65

>gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima]
          Length = 784

 Score =  299 bits (765), Expect = 3e-79
 Identities = 143/203 (70%), Positives = 169/203 (83%)
 Frame = +3

Query: 6   LEKQNLWSSRFLEERYSNGSIHLDRYSRLIFQEVDDTLKFPFRSNLERMVNRRNIEQYDA 185
           LEKQN W+S+FL +  SNGSIH D  ++ + QEVDD LKFP+ ++LER+ NRR IE Y+ 
Sbjct: 379 LEKQNFWTSQFLIQELSNGSIHADGLNKYVSQEVDDALKFPYHASLERLSNRRAIENYNK 438

Query: 186 NSTQILKTSYCSPNISNTGFLRLAVEDFNVCQSIQQEELKLLESWVVESSLDKLKFARQK 365
           N+T++LKT+Y S NI N  FL LAVEDFN+CQSIQ+EELK L  W+ E+ LDKLKFARQK
Sbjct: 439 NNTRVLKTAYSSSNIGNEDFLNLAVEDFNICQSIQREELKDLARWITENRLDKLKFARQK 498

Query: 366 TAYCYFSAAATLFSPELGDARISWAKNGILTTVVDDFFDIGGSIGELLNLIQLVEKWNVN 545
            AYCYFSAAATLFSPEL DARISWAKNG+LTTVVDDFFD+GGS+ EL+NLIQLVEKW+V+
Sbjct: 499 LAYCYFSAAATLFSPELSDARISWAKNGVLTTVVDDFFDVGGSVEELVNLIQLVEKWDVD 558

Query: 546 EETECYSENVRIIFSALHDTICD 614
             T+C SENV IIFSALH TICD
Sbjct: 559 VSTDCCSENVEIIFSALHSTICD 581


>ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chloroplastic-like [Vitis
           vinifera]
          Length = 793

 Score =  279 bits (713), Expect = 3e-73
 Identities = 134/204 (65%), Positives = 163/204 (79%)
 Frame = +3

Query: 6   LEKQNLWSSRFLEERYSNGSIHLDRYSRLIFQEVDDTLKFPFRSNLERMVNRRNIEQYDA 185
           LEKQN WSS FL +  SN SIH DR ++ I QEV+D L+FP+ +NL+R+ NRR+IE Y+ 
Sbjct: 388 LEKQNSWSSHFLRQGLSNSSIHADRLNKYIAQEVEDALRFPYYANLDRIANRRSIEHYNV 447

Query: 186 NSTQILKTSYCSPNISNTGFLRLAVEDFNVCQSIQQEELKLLESWVVESSLDKLKFARQK 365
           + T+ILKT+Y S ++ N  FL+LAVEDFN CQSI Q ELK LE W++E+ LDKLKFARQK
Sbjct: 448 DDTRILKTAYRSSHVCNKDFLKLAVEDFNFCQSIHQNELKQLERWIIENRLDKLKFARQK 507

Query: 366 TAYCYFSAAATLFSPELGDARISWAKNGILTTVVDDFFDIGGSIGELLNLIQLVEKWNVN 545
            AYCYFSAAAT+FSPE  DAR+SWAKN +LTTVVDDFFDIGGS  ELLNLIQLVEKW+++
Sbjct: 508 LAYCYFSAAATIFSPEQSDARLSWAKNSVLTTVVDDFFDIGGSEEELLNLIQLVEKWDID 567

Query: 546 EETECYSENVRIIFSALHDTICDI 617
              +C SE V I+FSALH TI +I
Sbjct: 568 VAVDCCSEQVEIVFSALHSTISEI 591


>emb|CBI24150.3| unnamed protein product [Vitis vinifera]
          Length = 826

 Score =  279 bits (713), Expect = 3e-73
 Identities = 134/204 (65%), Positives = 163/204 (79%)
 Frame = +3

Query: 6    LEKQNLWSSRFLEERYSNGSIHLDRYSRLIFQEVDDTLKFPFRSNLERMVNRRNIEQYDA 185
            LEKQN WSS FL +  SN SIH DR ++ I QEV+D L+FP+ +NL+R+ NRR+IE Y+ 
Sbjct: 421  LEKQNSWSSHFLRQGLSNSSIHADRLNKYIAQEVEDALRFPYYANLDRIANRRSIEHYNV 480

Query: 186  NSTQILKTSYCSPNISNTGFLRLAVEDFNVCQSIQQEELKLLESWVVESSLDKLKFARQK 365
            + T+ILKT+Y S ++ N  FL+LAVEDFN CQSI Q ELK LE W++E+ LDKLKFARQK
Sbjct: 481  DDTRILKTAYRSSHVCNKDFLKLAVEDFNFCQSIHQNELKQLERWIIENRLDKLKFARQK 540

Query: 366  TAYCYFSAAATLFSPELGDARISWAKNGILTTVVDDFFDIGGSIGELLNLIQLVEKWNVN 545
             AYCYFSAAAT+FSPE  DAR+SWAKN +LTTVVDDFFDIGGS  ELLNLIQLVEKW+++
Sbjct: 541  LAYCYFSAAATIFSPEQSDARLSWAKNSVLTTVVDDFFDIGGSEEELLNLIQLVEKWDID 600

Query: 546  EETECYSENVRIIFSALHDTICDI 617
               +C SE V I+FSALH TI +I
Sbjct: 601  VAVDCCSEQVEIVFSALHSTISEI 624


>gb|AAD34295.1|AF097311_1 kaurene synthase [Stevia rebaudiana]
          Length = 784

 Score =  256 bits (655), Expect = 2e-66
 Identities = 134/200 (67%), Positives = 159/200 (79%)
 Frame = +3

Query: 12  KQNLWSSRFLEERYSNGSIHLDRYSRLIFQEVDDTLKFPFRSNLERMVNRRNIEQYDANS 191
           KQ L S+ FL+E  S  S   +R S+LI +EV++ LKFP  + LER+  RRNI+ Y+ ++
Sbjct: 379 KQILKSADFLKEIISTDS---NRLSKLIHKEVENALKFPINTGLERINTRRNIQLYNVDN 435

Query: 192 TQILKTSYCSPNISNTGFLRLAVEDFNVCQSIQQEELKLLESWVVESSLDKLKFARQKTA 371
           T+ILKT+Y S NISNT +LRLAVEDF  CQSI +EELK LE WVVE+ LD+LKFARQKTA
Sbjct: 436 TRILKTTYHSSNISNTDYLRLAVEDFYTCQSIYREELKGLERWVVENKLDQLKFARQKTA 495

Query: 372 YCYFSAAATLFSPELGDARISWAKNGILTTVVDDFFDIGGSIGELLNLIQLVEKWNVNEE 551
           YCYFS AATL SPEL DARISWAKNGILTTVVDDFFDIGG+I EL NLIQ VEKWNV+ +
Sbjct: 496 YCYFSVAATLSSPELSDARISWAKNGILTTVVDDFFDIGGTIDELTNLIQCVEKWNVDVD 555

Query: 552 TECYSENVRIIFSALHDTIC 611
            +C SE+VRI+F AL D IC
Sbjct: 556 KDCCSEHVRILFLALKDAIC 575


>gb|AAD34294.1|AF097310_1 kaurene synthase [Stevia rebaudiana]
          Length = 784

 Score =  252 bits (643), Expect = 4e-65
 Identities = 132/200 (66%), Positives = 158/200 (79%)
 Frame = +3

Query: 12  KQNLWSSRFLEERYSNGSIHLDRYSRLIFQEVDDTLKFPFRSNLERMVNRRNIEQYDANS 191
           KQ L S+ FL+   S  S   +R S+LI +EV++ LKFP  + LER+  RRNI+ Y+ ++
Sbjct: 379 KQILKSADFLKGILSTDS---NRLSKLIHKEVENALKFPINTGLERINTRRNIQLYNVDN 435

Query: 192 TQILKTSYCSPNISNTGFLRLAVEDFNVCQSIQQEELKLLESWVVESSLDKLKFARQKTA 371
           T+ILKT+Y S NISNT +LRLAVEDF  CQSI +EELK LE WVV++ LD+LKFARQKTA
Sbjct: 436 TRILKTTYHSSNISNTYYLRLAVEDFYTCQSIYREELKGLERWVVQNKLDQLKFARQKTA 495

Query: 372 YCYFSAAATLFSPELGDARISWAKNGILTTVVDDFFDIGGSIGELLNLIQLVEKWNVNEE 551
           YCYFS AATL SPEL DARISWAKNGILTTVVDDFFDIGG+I EL NLIQ VEKWNV+ +
Sbjct: 496 YCYFSVAATLSSPELSDARISWAKNGILTTVVDDFFDIGGTIDELTNLIQCVEKWNVDVD 555

Query: 552 TECYSENVRIIFSALHDTIC 611
            +C SE+VRI+F AL D IC
Sbjct: 556 KDCCSEHVRILFLALKDAIC 575