BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00033735
         (424 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAR92224.1| cystatin [Actinidia deliciosa]                          99   3e-19
sp|Q6TPK4.1|CYT1_ACTDE RecName: Full=Cysteine proteinase inhibit...    89   3e-16
gb|AEQ54768.1| cysteine proteinase inhibitor CPI-3 [Coffea canep...    89   5e-16
gb|AAR92225.1| cystatin [Actinidia eriantha]                           88   8e-16
gb|AAG38521.1|AF283536_1 cystatin-like protein [Citrus x paradisi]     87   2e-15

>gb|AAR92224.1| cystatin [Actinidia deliciosa]
          Length = 115

 Score = 99.4 bits (246), Expect = 3e-19
 Identities = 57/116 (49%), Positives = 74/116 (63%), Gaps = 2/116 (1%)
 Frame = -1

Query: 358 LVTLTFSILHFLSDASAVI--SRRKVLLGGYLPIKDPKVSEVQIIGKFAVDEHNKEAKTN 185
           +V  TFS+L  L   SA +   R++V+LGG+ PIKD   +EVQ + +FAV EHNK+A   
Sbjct: 1   MVPKTFSMLLLLIALSAAVVGGRKQVVLGGWRPIKDLNSAEVQDVAQFAVSEHNKQANDK 60

Query: 184 LSYLKVVKGEKQVVAGINYRLIVEAKDGNNVLQHYEAVVYIKAGTNFMKLLSFGSI 17
           L Y +VV+G  QVVAG NYRL++ AKDG  VL  YEA V+ K    F  L SF  +
Sbjct: 61  LQYQRVVRGYSQVVAGTNYRLVIAAKDG-AVLGKYEAFVWDKPWMQFRNLTSFRKV 115


>sp|Q6TPK4.1|CYT1_ACTDE RecName: Full=Cysteine proteinase inhibitor 1; Short=KCPI1;
           AltName: Full=Phytocystatin; AltName: Allergen=Act d 4;
           Flags: Precursor gi|40807635|gb|AAR92223.1|
           phytocystatin [Actinidia deliciosa]
          Length = 116

 Score = 89.4 bits (220), Expect = 3e-16
 Identities = 53/117 (45%), Positives = 71/117 (60%)
 Frame = -1

Query: 367 LFLLVTLTFSILHFLSDASAVISRRKVLLGGYLPIKDPKVSEVQIIGKFAVDEHNKEAKT 188
           LFLL+ L+         A+ V  R+ V  GG+ PI+    +EVQ + +FAV EHNK+A  
Sbjct: 10  LFLLLALS---------AAVVGGRKLVAAGGWRPIESLNSAEVQDVAQFAVSEHNKQAND 60

Query: 187 NLSYLKVVKGEKQVVAGINYRLIVEAKDGNNVLQHYEAVVYIKAGTNFMKLLSFGSI 17
            L Y  VV+G  QVVAG NYRL++ AKDG  V+ +YEAVV+ K   +F  L SF  +
Sbjct: 61  ELQYQSVVRGYTQVVAGTNYRLVIAAKDG-AVVGNYEAVVWDKPWMHFRNLTSFRKV 116


>gb|AEQ54768.1| cysteine proteinase inhibitor CPI-3 [Coffea canephora]
          Length = 124

 Score = 88.6 bits (218), Expect = 5e-16
 Identities = 55/122 (45%), Positives = 72/122 (59%), Gaps = 2/122 (1%)
 Frame = -1

Query: 385 MVSNFQLFLLVTLTFSILHFLSDASAVISRRK--VLLGGYLPIKDPKVSEVQIIGKFAVD 212
           M S F   LL+T   +I  F    SA +  R    L+GG+    DPK  EV   GKFA+D
Sbjct: 1   MASAFPHLLLLTTLAAICLFSDVPSAALGGRPKDALVGGWSKA-DPKDPEVLENGKFAID 59

Query: 211 EHNKEAKTNLSYLKVVKGEKQVVAGINYRLIVEAKDGNNVLQHYEAVVYIKAGTNFMKLL 32
           EHNKEA T L +  VV+ +KQVVAG NY+++++A DG      YEA+V++K    F KL 
Sbjct: 60  EHNKEAGTKLEFKTVVEAQKQVVAGTNYKIVIKALDG-TASNLYEAIVWVKPWLKFKKLT 118

Query: 31  SF 26
           SF
Sbjct: 119 SF 120


>gb|AAR92225.1| cystatin [Actinidia eriantha]
          Length = 115

 Score = 87.8 bits (216), Expect = 8e-16
 Identities = 52/117 (44%), Positives = 72/117 (61%)
 Frame = -1

Query: 367 LFLLVTLTFSILHFLSDASAVISRRKVLLGGYLPIKDPKVSEVQIIGKFAVDEHNKEAKT 188
           LFLL+ L+         A+ V  R+ V  GG+ PI++   +EVQ + +FAV EHNK+A  
Sbjct: 9   LFLLLALS---------AAVVGGRKLVAPGGWRPIENLNSAEVQDVAQFAVSEHNKQAND 59

Query: 187 NLSYLKVVKGEKQVVAGINYRLIVEAKDGNNVLQHYEAVVYIKAGTNFMKLLSFGSI 17
            L Y  VV+G  QVV+G NYRL++ AKDG  V+ +YEAVV+ K   +F  L SF  +
Sbjct: 60  ELQYQSVVRGYTQVVSGTNYRLVIAAKDG-AVVGNYEAVVWDKPWMHFRNLTSFRKV 115


>gb|AAG38521.1|AF283536_1 cystatin-like protein [Citrus x paradisi]
          Length = 118

 Score = 86.7 bits (213), Expect = 2e-15
 Identities = 51/117 (43%), Positives = 72/117 (61%)
 Frame = -1

Query: 376 NFQLFLLVTLTFSILHFLSDASAVISRRKVLLGGYLPIKDPKVSEVQIIGKFAVDEHNKE 197
           N +   L+ L  S++  L    A   R+  L+GG+ PI+DPK   V  IG+FAV E+NK+
Sbjct: 2   NQRFCCLIVLFLSVVPLL----AAGDRKGALVGGWKPIEDPKEKHVMEIGQFAVTEYNKQ 57

Query: 196 AKTNLSYLKVVKGEKQVVAGINYRLIVEAKDGNNVLQHYEAVVYIKAGTNFMKLLSF 26
           +K+ L +  V KGE QVV+G NYRLI+  KDG +  + +EAVV+ K   +F  L SF
Sbjct: 58  SKSALKFESVEKGETQVVSGTNYRLILVVKDGPST-KKFEAVVWEKPWEHFKSLTSF 113