BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00031548
         (943 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004170243.1| PREDICTED: mechanosensitive ion channel prot...   121   2e-42
ref|XP_004140046.1| PREDICTED: mechanosensitive ion channel prot...   121   2e-42
sp|F4IME2.2|MSL8_ARATH RecName: Full=Mechanosensitive ion channe...   120   6e-40
ref|XP_004144925.1| PREDICTED: mechanosensitive ion channel prot...   112   8e-40
ref|XP_002305537.1| predicted protein [Populus trichocarpa] gi|2...   119   5e-39

>ref|XP_004170243.1| PREDICTED: mechanosensitive ion channel protein 8-like [Cucumis
           sativus]
          Length = 923

 Score =  121 bits (304), Expect(2) = 2e-42
 Identities = 62/146 (42%), Positives = 93/146 (63%), Gaps = 4/146 (2%)
 Frame = +2

Query: 329 SFSYENKLTRMKSRSRLVDPPDEYGNCK-RTMKSGFIGNGSESXXXXXX---PLLEQDFP 496
           SF  ++ L R K++SRL+DPP+       R  KSG + +G  S         P LE+D P
Sbjct: 238 SFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLP 297

Query: 497 QDYKKIVISKLAVIQFLSMFLTVAAIVCSLTVNISEKNELLGLELWRWGVLFLTLISGRL 676
            +YKK  +  L ++Q+ S+ L +AA+VC+LT+    + +L  LE+W+W V+ L LI GRL
Sbjct: 298 DEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRL 357

Query: 677 VAGWGIRVVVFLVQLNCMLRKRLHFF 754
           V+GWGIRV+VF ++ N +LRKR+ +F
Sbjct: 358 VSGWGIRVIVFFIERNFLLRKRVLYF 383



 Score = 77.8 bits (190), Expect(2) = 2e-42
 Identities = 34/65 (52%), Positives = 45/65 (69%)
 Frame = +1

Query: 748 FLLYGLRNAVKNCLWLALVLFSWRCLLVDRVEKLAHGKVFGYVTRSWVCLLVGTLIWFVK 927
           + +YG+R AV+NCLWL LVL +W  L  D+V++        YVT+  VCLLV TL+W VK
Sbjct: 382 YFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVK 441

Query: 928 TLLVK 942
           TL+VK
Sbjct: 442 TLMVK 446


>ref|XP_004140046.1| PREDICTED: mechanosensitive ion channel protein 8-like [Cucumis
           sativus]
          Length = 923

 Score =  121 bits (304), Expect(2) = 2e-42
 Identities = 62/146 (42%), Positives = 93/146 (63%), Gaps = 4/146 (2%)
 Frame = +2

Query: 329 SFSYENKLTRMKSRSRLVDPPDEYGNCK-RTMKSGFIGNGSESXXXXXX---PLLEQDFP 496
           SF  ++ L R K++SRL+DPP+       R  KSG + +G  S         P LE+D P
Sbjct: 238 SFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLP 297

Query: 497 QDYKKIVISKLAVIQFLSMFLTVAAIVCSLTVNISEKNELLGLELWRWGVLFLTLISGRL 676
            +YKK  +  L ++Q+ S+ L +AA+VC+LT+    + +L  LE+W+W V+ L LI GRL
Sbjct: 298 DEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRL 357

Query: 677 VAGWGIRVVVFLVQLNCMLRKRLHFF 754
           V+GWGIRV+VF ++ N +LRKR+ +F
Sbjct: 358 VSGWGIRVIVFFIERNFLLRKRVLYF 383



 Score = 77.8 bits (190), Expect(2) = 2e-42
 Identities = 34/65 (52%), Positives = 45/65 (69%)
 Frame = +1

Query: 748 FLLYGLRNAVKNCLWLALVLFSWRCLLVDRVEKLAHGKVFGYVTRSWVCLLVGTLIWFVK 927
           + +YG+R AV+NCLWL LVL +W  L  D+V++        YVT+  VCLLV TL+W VK
Sbjct: 382 YFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVK 441

Query: 928 TLLVK 942
           TL+VK
Sbjct: 442 TLMVK 446


>sp|F4IME2.2|MSL8_ARATH RecName: Full=Mechanosensitive ion channel protein 8; AltName:
           Full=Mechanosensitive channel of small conductance-like
           8; AltName: Full=MscS-Like protein 8
          Length = 908

 Score =  120 bits (300), Expect(2) = 6e-40
 Identities = 76/250 (30%), Positives = 127/250 (50%), Gaps = 2/250 (0%)
 Frame = +2

Query: 11  DVVINMNDNNYNETHHEVASSTKIHGGSSNTSCNTHSDSVNESPAAHNYSYSESPTLYGQ 190
           ++ ++++  N + +H  + + T        ++  T  D+  E   + N   +    + G 
Sbjct: 142 EITLDVDQENDDVSHQTMPTPT--------STARTSFDASREMRVSFNVRRAGGAFVAGS 193

Query: 191 ASSPRDHVKVSFTDSPRNNLYGTTNRAGGGCPSGRREEWFARASNKSFSYENKL-TRMKS 367
             S   H   S + + R N            P  + EE     SN SF  +++L +R+K+
Sbjct: 194 VPSSSSHSSSSSSATMRTNQ---------DQPQLQEEEVVRCTSNMSFQRKSELISRVKT 244

Query: 368 RSRLVDPPDEYGNCKRTMKSGFIGNGS-ESXXXXXXPLLEQDFPQDYKKIVISKLAVIQF 544
           RSRL DPP E        +SG + +G          PL E+D P +YK+  +  + ++Q+
Sbjct: 245 RSRLQDPPREEETPYSGWRSGQLKSGLLADIDEEDDPLAEEDVPDEYKRGKLDAITLLQW 304

Query: 545 LSMFLTVAAIVCSLTVNISEKNELLGLELWRWGVLFLTLISGRLVAGWGIRVVVFLVQLN 724
           LS+   +AA+ CSL++   +K  +  L LW+W V  L LI GRLV+GWGIR+VVF ++ N
Sbjct: 305 LSLVAIIAALACSLSIQSWKKVRVWNLHLWKWEVFLLVLICGRLVSGWGIRIVVFFIERN 364

Query: 725 CMLRKRLHFF 754
            +LRKR+ +F
Sbjct: 365 FLLRKRVLYF 374



 Score = 71.2 bits (173), Expect(2) = 6e-40
 Identities = 29/65 (44%), Positives = 44/65 (67%)
 Frame = +1

Query: 748 FLLYGLRNAVKNCLWLALVLFSWRCLLVDRVEKLAHGKVFGYVTRSWVCLLVGTLIWFVK 927
           + +YG+R AV+NCLWL LVL +W  L   +V++    +   YVT+  VC L+ T++W +K
Sbjct: 373 YFVYGVRRAVQNCLWLGLVLLAWHFLFDKKVQRETRSRFLPYVTKILVCFLLSTILWLIK 432

Query: 928 TLLVK 942
           TL+VK
Sbjct: 433 TLVVK 437


>ref|XP_004144925.1| PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis
           sativus] gi|449529323|ref|XP_004171649.1| PREDICTED:
           mechanosensitive ion channel protein 6-like [Cucumis
           sativus]
          Length = 955

 Score =  112 bits (279), Expect(2) = 8e-40
 Identities = 64/179 (35%), Positives = 98/179 (54%), Gaps = 16/179 (8%)
 Frame = +2

Query: 266 RAGGGCPSGRREEWFARA----------SNKSFSYENKLTRMKSRSRLVDPPDEYGNCKR 415
           R G    SG + E  A A          S  SF  ++ L R K++SRL+DPP E      
Sbjct: 235 RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSG 294

Query: 416 T------MKSGFIGNGSESXXXXXXPLLEQDFPQDYKKIVISKLAVIQFLSMFLTVAAIV 577
                  ++SGF+G   +       P LE D P ++K+   S L V+Q++S+ +  AA++
Sbjct: 295 LIPKSGQLRSGFLGKIEDDDDD---PFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALI 351

Query: 578 CSLTVNISEKNELLGLELWRWGVLFLTLISGRLVAGWGIRVVVFLVQLNCMLRKRLHFF 754
           C+L+V    +  L  L++W+W V+   LI GRLV+GWGIR+ VF ++ N +LRKR+ +F
Sbjct: 352 CTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYF 410



 Score = 79.0 bits (193), Expect(2) = 8e-40
 Identities = 35/65 (53%), Positives = 45/65 (69%)
 Frame = +1

Query: 748 FLLYGLRNAVKNCLWLALVLFSWRCLLVDRVEKLAHGKVFGYVTRSWVCLLVGTLIWFVK 927
           + +YG+R  V+NCLWL LVL +W  L   RVEK  +  +  YV+R  VCLL+ TLIW VK
Sbjct: 409 YFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVK 468

Query: 928 TLLVK 942
           TL+VK
Sbjct: 469 TLMVK 473


>ref|XP_002305537.1| predicted protein [Populus trichocarpa] gi|222848501|gb|EEE86048.1|
           predicted protein [Populus trichocarpa]
          Length = 700

 Score =  119 bits (299), Expect(2) = 5e-39
 Identities = 68/161 (42%), Positives = 103/161 (63%), Gaps = 8/161 (4%)
 Frame = +2

Query: 296 REEWFARASNKSFS-YENKLTRMKSRSRLVDPPDEYGNCK---RTMKSGFIGNGSESXXX 463
           REE    +SN SF  + N L+R+K++SRL+D P      +   R  KSG + +G  S   
Sbjct: 8   REEVLKCSSNASFRRHANPLSRLKTKSRLIDDPSPQELERMSGRIPKSGPMRSGMLSRAL 67

Query: 464 XXX----PLLEQDFPQDYKKIVISKLAVIQFLSMFLTVAAIVCSLTVNISEKNELLGLEL 631
                  PL + D P++YKK  +S L V+Q+LS+ + +AA+VCSL++   +K ++L L+L
Sbjct: 68  YDEDDEDPLEDVDLPEEYKKDKLSTLTVLQWLSLIVILAALVCSLSIRDLKKVKILNLKL 127

Query: 632 WRWGVLFLTLISGRLVAGWGIRVVVFLVQLNCMLRKRLHFF 754
           W+W VL L LI GRLV+GWGI ++VF ++ N +LRKR+ +F
Sbjct: 128 WKWEVLLLVLICGRLVSGWGIHLIVFFIERNFLLRKRVLYF 168



 Score = 68.6 bits (166), Expect(2) = 5e-39
 Identities = 30/65 (46%), Positives = 40/65 (61%)
 Frame = +1

Query: 748 FLLYGLRNAVKNCLWLALVLFSWRCLLVDRVEKLAHGKVFGYVTRSWVCLLVGTLIWFVK 927
           + +YGLR  V+NC WL LVL +W  L   +V++        YVT+  VC LVG  IW +K
Sbjct: 167 YFVYGLRKGVQNCWWLGLVLLAWHFLFDKKVQRDTKSDFLEYVTKILVCFLVGNFIWLIK 226

Query: 928 TLLVK 942
           TL+VK
Sbjct: 227 TLMVK 231