BLASTX nr result
ID: Bupleurum21_contig00031341
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00031341
(1298 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_001234176.1| PI-phospholipase C PLC5 [Solanum lycopersicu... 325 2e-86
ref|XP_003632177.1| PREDICTED: phosphoinositide phospholipase C ... 316 7e-84
ref|XP_002278527.1| PREDICTED: phosphoinositide phospholipase C ... 316 7e-84
ref|XP_002278839.2| PREDICTED: phosphoinositide phospholipase C ... 314 3e-83
gb|AFO54605.1| phospholipase C [Populus tomentosa] 313 5e-83
>ref|NP_001234176.1| PI-phospholipase C PLC5 [Solanum lycopersicum]
gi|158827644|gb|ABW80999.1| PI-phospholipase C PLC5
[Solanum lycopersicum]
Length = 584
Score = 325 bits (832), Expect = 2e-86
Identities = 180/334 (53%), Positives = 219/334 (65%), Gaps = 4/334 (1%)
Frame = -3
Query: 1281 MFGLFNRKFKVKESGPPSDVVEAFSAYTEGRSYMSAGQFRRFLVEFQGEVEFTLEMAEQL 1102
MFG FNRKFK++E PP DV AF YT + M+A Q R+LVE QGE E T++ AEQ+
Sbjct: 1 MFGCFNRKFKIREREPPPDVRNAFFRYTGKANQMNADQLFRYLVEVQGEEECTIKDAEQI 60
Query: 1101 MRQVLHQRHHLTKYTRXXXXXXXXXXXXXXXXLNSPINLQVHHDMTAPLQHYFIYTGHNS 922
++ V +RHHL + LN I QVHHDMTAPLQHYFIYTGHNS
Sbjct: 61 IQNVASRRHHLIRRLNHSLELDDFFFYLFQDDLNGAIKSQVHHDMTAPLQHYFIYTGHNS 120
Query: 921 YLTGNQLSSDSSDIPIINALERGVRGIELDLWPNSTNDGIHVTHGRTLTTPVTLERCLMS 742
YLTGNQLSSD S+IPI+ ALERGVRGIELDLWPNS D IHV HGRTLTTPV L +CL +
Sbjct: 121 YLTGNQLSSDCSEIPIVKALERGVRGIELDLWPNSGKDNIHVLHGRTLTTPVPLLKCLKA 180
Query: 741 IKQYAFVASSYPVIITLEDHLTPSLRAKVAEMAVEIFGELLYRPEPACYNVLPSPEALKH 562
I+ +AF S YPVIITLEDHLTP L+AKVAEM ++IFGE+LY P+ C + PSPE LK+
Sbjct: 181 IRDHAFFKSPYPVIITLEDHLTPDLQAKVAEMVIQIFGEMLYYPQSECLDEFPSPEELKN 240
Query: 561 QIILSTKVPKECTESMNINDNENELSTEELSGEDLSEMETPKL-ADDLQTDRWNSYSNLG 385
+IILSTK PKE ES N D ++ EDL + E ++ A+D TD S S+
Sbjct: 241 RIILSTKPPKEYLESKNQRDTSP--VGKDSFREDLLKKEKSEIGAEDHDTDE-RSDSDQD 297
Query: 384 DEE---HGSDRHRSSQQDGAMYKHLFSYKSADRK 292
DE+ S+ +SSQ + YK L + + K
Sbjct: 298 DEDGDTSTSNDQQSSQPEAPKYKSLIAVHAGKAK 331
>ref|XP_003632177.1| PREDICTED: phosphoinositide phospholipase C 4-like isoform 4 [Vitis
vinifera]
Length = 559
Score = 316 bits (810), Expect = 7e-84
Identities = 167/333 (50%), Positives = 215/333 (64%), Gaps = 1/333 (0%)
Frame = -3
Query: 1287 YKMFGLFNRKFKVKESGPPSDVVEAFSAYTEGRSYMSAGQFRRFLVEFQGEVEFTLEMAE 1108
Y++ F RKF+V E+ PP DV EAF Y EG ++M+A Q RRFL+E Q + TL AE
Sbjct: 4 YRVCMCFRRKFRVVEAEPPEDVKEAFKKYAEGGTHMTAEQLRRFLLESQSDGSVTLSDAE 63
Query: 1107 QLMRQVLHQRHHLTKYTRXXXXXXXXXXXXXXXXLNSPINLQVHHDMTAPLQHYFIYTGH 928
+++ QVL +RHH+ K+TR LN+PI QVH DM APL HYFIYTGH
Sbjct: 64 RIVEQVLQKRHHIAKFTRHNLTLDDFHHFLFSTDLNAPIGSQVHQDMNAPLSHYFIYTGH 123
Query: 927 NSYLTGNQLSSDSSDIPIINALERGVRGIELDLWPNSTNDGIHVTHGRTLTTPVTLERCL 748
NSYLTGNQLSSD SD+P+I AL+RGVR IELD+WP+ST +HV HGRTLTTPV L +CL
Sbjct: 124 NSYLTGNQLSSDCSDVPVIKALKRGVRVIELDIWPDSTKADVHVLHGRTLTTPVELIKCL 183
Query: 747 MSIKQYAFVASSYPVIITLEDHLTPSLRAKVAEMAVEIFGELLYRPEPACYNVLPSPEAL 568
SIK++AF AS YPVIITLEDHLTP L+AKVA+M + F ++L+ P+ C PSPE L
Sbjct: 184 RSIKEHAFSASPYPVIITLEDHLTPDLQAKVAQMVTQTFQDILFYPKSECLQEFPSPEEL 243
Query: 567 KHQIILSTKVPKECTESMNINDNENELSTEELSGEDLSEMETPKL-ADDLQTDRWNSYSN 391
K++II+STK PKE E+ D EN + + S +D+ E + AD D+ +S +
Sbjct: 244 KYRIIISTKPPKEYLEAKGSEDKENNIQKGKDSDDDVWGEEPSNITADHENNDKSDSEGS 303
Query: 390 LGDEEHGSDRHRSSQQDGAMYKHLFSYKSADRK 292
DE+ G + + YKHL S + K
Sbjct: 304 EVDED-GEENNDCRPIGAPAYKHLISIHAGKPK 335
>ref|XP_002278527.1| PREDICTED: phosphoinositide phospholipase C 4-like isoform 1 [Vitis
vinifera]
Length = 588
Score = 316 bits (810), Expect = 7e-84
Identities = 167/333 (50%), Positives = 215/333 (64%), Gaps = 1/333 (0%)
Frame = -3
Query: 1287 YKMFGLFNRKFKVKESGPPSDVVEAFSAYTEGRSYMSAGQFRRFLVEFQGEVEFTLEMAE 1108
Y++ F RKF+V E+ PP DV EAF Y EG ++M+A Q RRFL+E Q + TL AE
Sbjct: 4 YRVCMCFRRKFRVVEAEPPEDVKEAFKKYAEGGTHMTAEQLRRFLLESQSDGSVTLSDAE 63
Query: 1107 QLMRQVLHQRHHLTKYTRXXXXXXXXXXXXXXXXLNSPINLQVHHDMTAPLQHYFIYTGH 928
+++ QVL +RHH+ K+TR LN+PI QVH DM APL HYFIYTGH
Sbjct: 64 RIVEQVLQKRHHIAKFTRHNLTLDDFHHFLFSTDLNAPIGSQVHQDMNAPLSHYFIYTGH 123
Query: 927 NSYLTGNQLSSDSSDIPIINALERGVRGIELDLWPNSTNDGIHVTHGRTLTTPVTLERCL 748
NSYLTGNQLSSD SD+P+I AL+RGVR IELD+WP+ST +HV HGRTLTTPV L +CL
Sbjct: 124 NSYLTGNQLSSDCSDVPVIKALKRGVRVIELDIWPDSTKADVHVLHGRTLTTPVELIKCL 183
Query: 747 MSIKQYAFVASSYPVIITLEDHLTPSLRAKVAEMAVEIFGELLYRPEPACYNVLPSPEAL 568
SIK++AF AS YPVIITLEDHLTP L+AKVA+M + F ++L+ P+ C PSPE L
Sbjct: 184 RSIKEHAFSASPYPVIITLEDHLTPDLQAKVAQMVTQTFQDILFYPKSECLQEFPSPEEL 243
Query: 567 KHQIILSTKVPKECTESMNINDNENELSTEELSGEDLSEMETPKL-ADDLQTDRWNSYSN 391
K++II+STK PKE E+ D EN + + S +D+ E + AD D+ +S +
Sbjct: 244 KYRIIISTKPPKEYLEAKGSEDKENNIQKGKDSDDDVWGEEPSNITADHENNDKSDSEGS 303
Query: 390 LGDEEHGSDRHRSSQQDGAMYKHLFSYKSADRK 292
DE+ G + + YKHL S + K
Sbjct: 304 EVDED-GEENNDCRPIGAPAYKHLISIHAGKPK 335
>ref|XP_002278839.2| PREDICTED: phosphoinositide phospholipase C 4-like [Vitis vinifera]
Length = 590
Score = 314 bits (805), Expect = 3e-83
Identities = 176/399 (44%), Positives = 240/399 (60%), Gaps = 23/399 (5%)
Frame = -3
Query: 1287 YKMFGLFNRKFKVKESGPPSDVVEAFSAYTEGRSYMSAGQFRRFLVEFQGEVEFTLEMAE 1108
Y + F RKF++ E+ PP+DV EAF YTEG ++M+A Q RFLVE Q +V T+ AE
Sbjct: 4 YSVCICFKRKFRLTEAEPPTDVKEAFRRYTEGGTHMTAEQLLRFLVEGQNDVAATISDAE 63
Query: 1107 QLMRQVLHQRHHLTKYTRXXXXXXXXXXXXXXXXLNSPINLQVHHDMTAPLQHYFIYTGH 928
+++ VLH+RHH+TK+ R LN PI QVH DM+APL HYFIYTGH
Sbjct: 64 RILELVLHRRHHVTKFARTTLTLDDFHHFLFSTDLNGPIGSQVHQDMSAPLSHYFIYTGH 123
Query: 927 NSYLTGNQLSSDSSDIPIINALERGVRGIELDLWPNSTNDGIHVTHGRTLTTPVTLERCL 748
NSYLTGNQL+S+ SD+PI+ AL+RGVR +ELDLWP ST + +HV HGRTLTTPV L +CL
Sbjct: 124 NSYLTGNQLNSNCSDVPIVKALKRGVRMVELDLWPGSTKENVHVLHGRTLTTPVELIKCL 183
Query: 747 MSIKQYAFVASSYPVIITLEDHLTPSLRAKVAEMAVEIFGELLYRPEPACYNVLPSPEAL 568
SIK++AF AS YPVIITLEDHLTP L+AKVA+M + FG++L+ P+ PSPE L
Sbjct: 184 KSIKEHAFSASPYPVIITLEDHLTPDLQAKVAQMISQTFGDMLFYPKSESLPEFPSPEEL 243
Query: 567 KHQIILSTKVPKECTESMNINDNENELSTEELSGEDLSEMETPKLADDLQTDRWNSYSNL 388
K+++I+STK PKE E+ + D N + S ED+S P L D ++ N S+
Sbjct: 244 KYRVIISTKPPKEYLEAKSSEDKVNNSQKGKDSDEDVSMKVPPDLTVDHES---NDKSDS 300
Query: 387 GDEEHGSDRHRSSQQ-----DGAMYKHLFSYKS--------ADRKVEPPNNLAGLA---- 259
G E S+ S+ + + YKHL + + A KVE PN ++ L+
Sbjct: 301 GRSEGSSENDESNDECDRPLEAPAYKHLIAIHAAKPSGGLKASLKVE-PNKVSRLSLNEQ 359
Query: 258 ------SIHNRNFIHSEAPDECSKHPRFDGYRFNLYPPV 160
+ H R+ + + +P+ + + Y P+
Sbjct: 360 AFGKAIAYHRRDVVRFTQKNLLRVYPKATRFNSSNYKPL 398
>gb|AFO54605.1| phospholipase C [Populus tomentosa]
Length = 586
Score = 313 bits (803), Expect = 5e-83
Identities = 171/333 (51%), Positives = 210/333 (63%), Gaps = 1/333 (0%)
Frame = -3
Query: 1287 YKMFGLFNRKFKVKESGPPSDVVEAFSAYTEGRSYMSAGQFRRFLVEFQGEVEFTLEMAE 1108
Y+M F RKFKV ++GPP DV EAF YT+G ++MSA Q RRFLVE QG+ + AE
Sbjct: 5 YRMCMCFTRKFKVTKAGPPHDVNEAFFKYTDGGTHMSAEQLRRFLVEVQGDGGVSTADAE 64
Query: 1107 QLMRQVLHQRHHLTKYTRXXXXXXXXXXXXXXXXLNSPINLQVHHDMTAPLQHYFIYTGH 928
+++ QVL + HH+ K+TR LN PI QVH DMT PL HYFIYTGH
Sbjct: 65 KIVDQVLQKMHHIAKFTRRTLTLDDFHHYLFSADLNPPIGDQVHQDMTKPLSHYFIYTGH 124
Query: 927 NSYLTGNQLSSDSSDIPIINALERGVRGIELDLWPNSTNDGIHVTHGRTLTTPVTLERCL 748
NSYLTGNQLSSD SD+PII AL+RGVR IELD+WPNST D + V HGRTLT PV L RCL
Sbjct: 125 NSYLTGNQLSSDCSDVPIIKALKRGVRVIELDIWPNSTKDNVDVLHGRTLTAPVELIRCL 184
Query: 747 MSIKQYAFVASSYPVIITLEDHLTPSLRAKVAEMAVEIFGELLYRPEPACYNVLPSPEAL 568
SIK+YAF +S YPVIITLEDHLTP L+ KVA+M E FG +LY PE C PSPE L
Sbjct: 185 KSIKEYAFSSSPYPVIITLEDHLTPDLQVKVAQMITETFGGMLYCPECECLEEFPSPEEL 244
Query: 567 KHQIILSTKVPKECTESMNINDNENELSTEELSGEDLSEMETPKLADDLQTDRWNSYSNL 388
K++II+STK PKE ++ D N+ ++ S +D E L D + ++
Sbjct: 245 KYRIIISTKPPKEYLKAEGGKDKGNKSRKDKDSDDDTWGKEPLDLVSDQED------GDV 298
Query: 387 GDEEHGSDRHRSSQQDG-AMYKHLFSYKSADRK 292
D + D SQQ G + YK L + + K
Sbjct: 299 SDTDTSEDSDGESQQPGVSAYKRLIAIHAGKPK 331