BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00029307
         (975 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABB52075.1| putative beta-ring carotene hydroxylase [Daucus c...   437   e-120
ref|XP_004140758.1| PREDICTED: beta-carotene 3-hydroxylase, chlo...   378   e-102
gb|AEK86567.1| chloroplast beta-carotene hydroxylase [Cucurbita ...   377   e-102
ref|XP_002273581.1| PREDICTED: beta-carotene hydroxylase 2, chlo...   376   e-102
gb|ADZ14893.1| beta-carotene hydroxylase [Carica papaya]              375   e-102

>gb|ABB52075.1| putative beta-ring carotene hydroxylase [Daucus carota subsp.
           sativus]
          Length = 303

 Score =  437 bits (1123), Expect = e-120
 Identities = 227/306 (74%), Positives = 247/306 (80%), Gaps = 12/306 (3%)
 Frame = -2

Query: 884 ARMTIGSYCIGKNTVQSPKLATTVMQLSPTTIYKRSGLFMGLT--RKQKLSLCRAVEESE 711
           AR+++G + +GK  +Q PK  T V      TI + S  F+GL    K+KLSLC AVEE+E
Sbjct: 3   ARLSVGLFRVGKYEIQVPKQMTVVK-----TIRELSAPFIGLRLGNKRKLSLCFAVEENE 57

Query: 710 GRVA----ESSSGEIEKHVILH------SRAEDRMARKKMERLTYLVAAVMSSFGITSMA 561
             VA    E SS E+EK +I        SRAE+RMARKK ER TYLVAAVMSSFGITSMA
Sbjct: 58  SPVAAAEAEESSREVEKQIIESFTVAGGSRAEERMARKKTERFTYLVAAVMSSFGITSMA 117

Query: 560 VLAVYSRFAWQMEGGEISYSEMFGTFSLAFGAAVGMEYWARWAHKELWHSSLWHMHESHH 381
           V AVYSRFAWQMEGGE+ +SEM  TFSLAFGAAVGMEYWARWAHK LWH SLWHMHESHH
Sbjct: 118 VFAVYSRFAWQMEGGEVPHSEMLATFSLAFGAAVGMEYWARWAHKALWHDSLWHMHESHH 177

Query: 380 KPREGAFEMNDVFAIINAVPAIALLAFGFFHKGLFPGLCFGAGLGITVFGMAYMFVHDGL 201
           KPREGAFE+NDVFAIINAVPAIALLA+GFFHKGLFPGLCFGAGLGITVFGMAYMFVHDGL
Sbjct: 178 KPREGAFELNDVFAIINAVPAIALLAYGFFHKGLFPGLCFGAGLGITVFGMAYMFVHDGL 237

Query: 200 VHKRFPVGPVANVPYLRKVAAAHQLHHMEKFNGVPYGLFLGPQXXXXXXXXXXXEKEISR 21
           VHKRFPVGPVANVPYLRKVAAAHQLHHMEKFNGVPYGLFLGPQ           EKEI+R
Sbjct: 238 VHKRFPVGPVANVPYLRKVAAAHQLHHMEKFNGVPYGLFLGPQELEQVGGNEELEKEINR 297

Query: 20  RMKSSS 3
           R+KSS+
Sbjct: 298 RIKSSN 303


>ref|XP_004140758.1| PREDICTED: beta-carotene 3-hydroxylase, chloroplastic-like [Cucumis
           sativus]
          Length = 310

 Score =  378 bits (970), Expect = e-102
 Identities = 188/269 (69%), Positives = 213/269 (79%), Gaps = 4/269 (1%)
 Frame = -2

Query: 806 LSPTTIYKRSGLFMGLTRKQKLSLCRAVE--ESEGRVAESSSGE--IEKHVILHSRAEDR 639
           L P ++++ S     + RK  L++C  VE   S G+V   S  E  I    I      +R
Sbjct: 32  LFPPSVFRNSRFQWKMRRKTLLTVCVLVEGQNSSGKVDNLSDEESPIVVPQIPSPHVSER 91

Query: 638 MARKKMERLTYLVAAVMSSFGITSMAVLAVYSRFAWQMEGGEISYSEMFGTFSLAFGAAV 459
           +ARKK ER TYLVAAVMS+FGITSMAV+AVY RF WQMEGGEI +SEMFGTFSL+ GAAV
Sbjct: 92  LARKKSERFTYLVAAVMSTFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV 151

Query: 458 GMEYWARWAHKELWHSSLWHMHESHHKPREGAFEMNDVFAIINAVPAIALLAFGFFHKGL 279
           GME+WARWAH+ LWHSSLWHMHESHHKPREG FE+ND+FAIINAVPAIALL++GFFHKGL
Sbjct: 152 GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHKGL 211

Query: 278 FPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPVANVPYLRKVAAAHQLHHMEKFNGV 99
            PGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGP+ANVPY RKVAAAHQLHH +KFNGV
Sbjct: 212 VPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGV 271

Query: 98  PYGLFLGPQXXXXXXXXXXXEKEISRRMK 12
           PYGLFLGP+           EKEI+RR+K
Sbjct: 272 PYGLFLGPKELEEVGGLEELEKEINRRIK 300


>gb|AEK86567.1| chloroplast beta-carotene hydroxylase [Cucurbita moschata]
          Length = 310

 Score =  377 bits (969), Expect = e-102
 Identities = 190/288 (65%), Positives = 222/288 (77%), Gaps = 10/288 (3%)
 Frame = -2

Query: 836 SPKLATTVMQLSPTTIYKRSGLFMGLTRKQKLSLCRAVEESEGRVAESSSGEIEKHV--- 666
           SPK  T +  L P+++++ S +   + RK  L++C  VE+      +SSS E+E      
Sbjct: 24  SPKPRTPL--LFPSSVFRNSRIQWKMRRKTLLTVCVLVED------QSSSSEVENLADEK 75

Query: 665 -------ILHSRAEDRMARKKMERLTYLVAAVMSSFGITSMAVLAVYSRFAWQMEGGEIS 507
                  I      +++ARKK ER TYLVAAVMSSFGITSMAV+AVY RF WQMEGGEI 
Sbjct: 76  LLIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMAVYYRFHWQMEGGEIP 135

Query: 506 YSEMFGTFSLAFGAAVGMEYWARWAHKELWHSSLWHMHESHHKPREGAFEMNDVFAIINA 327
           +SEMFGTF+L+ GAAVGME+WARWAH+ LWH+SLWHMHESHHKPREG FE+NDVFAI NA
Sbjct: 136 FSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAITNA 195

Query: 326 VPAIALLAFGFFHKGLFPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPVANVPYLRK 147
           VPAIALL++GFFHKGL PGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGP+ANVPY RK
Sbjct: 196 VPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRK 255

Query: 146 VAAAHQLHHMEKFNGVPYGLFLGPQXXXXXXXXXXXEKEISRRMKSSS 3
           VAAAHQLHH EK NGVPYGLFLGP+           EKEI+RR+KS++
Sbjct: 256 VAAAHQLHHSEKLNGVPYGLFLGPKELEEVGGLEELEKEINRRIKSTA 303


>ref|XP_002273581.1| PREDICTED: beta-carotene hydroxylase 2, chloroplastic isoform 1
           [Vitis vinifera]
          Length = 306

 Score =  376 bits (966), Expect = e-102
 Identities = 187/291 (64%), Positives = 224/291 (76%), Gaps = 6/291 (2%)
 Frame = -2

Query: 857 IGKNTVQSPKLATTVMQLSPTTIY----KRSGLFMGLTRKQKLSLCRAVEESE--GRVAE 696
           +G+N    PK  +     +PT+++    +R        +K +L++C  VEE +    V E
Sbjct: 16  LGRNPFLGPKPTSP---FTPTSLFIPSIRRHENIFRCRKKTRLTVCFVVEEEKLSTEVVE 72

Query: 695 SSSGEIEKHVILHSRAEDRMARKKMERLTYLVAAVMSSFGITSMAVLAVYSRFAWQMEGG 516
           + S E     I  +R  +++ARK+ ERLTYLVAAVMSSFGITSMAV+AVY RF+WQMEGG
Sbjct: 73  NRSEETLASQISAARVAEKLARKRSERLTYLVAAVMSSFGITSMAVMAVYYRFSWQMEGG 132

Query: 515 EISYSEMFGTFSLAFGAAVGMEYWARWAHKELWHSSLWHMHESHHKPREGAFEMNDVFAI 336
           E+  SEMFGTF+L+ GAAVGME+WARWAHK LWH+SLWHMHESHHKPREG FE+NDVFAI
Sbjct: 133 EVPLSEMFGTFALSVGAAVGMEFWARWAHKALWHASLWHMHESHHKPREGPFELNDVFAI 192

Query: 335 INAVPAIALLAFGFFHKGLFPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPVANVPY 156
            NAVPAIALL++GFFHKGL PGLCFGAGLGITVFGMAYMFVHDGLVH+RFPVGP+ANVPY
Sbjct: 193 TNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIANVPY 252

Query: 155 LRKVAAAHQLHHMEKFNGVPYGLFLGPQXXXXXXXXXXXEKEISRRMKSSS 3
            R+VAAAHQLHH +KFNGVPYGLFLGP+           EKEI+RR + S+
Sbjct: 253 FRRVAAAHQLHHSDKFNGVPYGLFLGPKEVEEVGGKDELEKEINRRARLSN 303


>gb|ADZ14893.1| beta-carotene hydroxylase [Carica papaya]
          Length = 305

 Score =  375 bits (963), Expect = e-102
 Identities = 187/274 (68%), Positives = 217/274 (79%), Gaps = 10/274 (3%)
 Frame = -2

Query: 800 PTTIYKRS-GLFMGLTRKQK---LSLCRAVEES------EGRVAESSSGEIEKHVILHSR 651
           PT I   S GL  G+ R  K    ++C  +EE       E +  E SS ++E   IL S+
Sbjct: 28  PTLISPLSRGLNRGIFRPHKRTTFAVCFVLEEQKQIAQIENQAEELSSKDVELEQILASK 87

Query: 650 AEDRMARKKMERLTYLVAAVMSSFGITSMAVLAVYSRFAWQMEGGEISYSEMFGTFSLAF 471
             +++ARK+ ER TYLVAAVMSSFGITSMAV+AVY RFAWQMEGGE+ +SEMFGTF+L+ 
Sbjct: 88  MAEKLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFAWQMEGGEVPFSEMFGTFALSV 147

Query: 470 GAAVGMEYWARWAHKELWHSSLWHMHESHHKPREGAFEMNDVFAIINAVPAIALLAFGFF 291
           GAAVGME+WARWAH+ LWH+SLWHMHESHH+PREG FE+NDVFAI NAVPAIALL++GFF
Sbjct: 148 GAAVGMEFWARWAHRALWHASLWHMHESHHRPREGPFELNDVFAITNAVPAIALLSYGFF 207

Query: 290 HKGLFPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPVANVPYLRKVAAAHQLHHMEK 111
           +KGL PGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGP+ANVPY R+VAAAHQLHH +K
Sbjct: 208 NKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRRVAAAHQLHHSDK 267

Query: 110 FNGVPYGLFLGPQXXXXXXXXXXXEKEISRRMKS 9
           FNGVPYGLFLGP+           EKEI+RR KS
Sbjct: 268 FNGVPYGLFLGPKEVEDVGGKEELEKEINRRRKS 301