BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00027764
         (422 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002511355.1| ubiquitin-protein ligase, putative [Ricinus ...    68   7e-10
ref|XP_004138258.1| PREDICTED: putative FBD-associated F-box pro...    68   9e-10
emb|CAN81846.1| hypothetical protein VITISV_037892 [Vitis vinifera]    59   5e-07
ref|XP_002513467.1| ubiquitin-protein ligase, putative [Ricinus ...    57   2e-06
emb|CBI20286.3| unnamed protein product [Vitis vinifera]               56   3e-06

>ref|XP_002511355.1| ubiquitin-protein ligase, putative [Ricinus communis]
           gi|223550470|gb|EEF51957.1| ubiquitin-protein ligase,
           putative [Ricinus communis]
          Length = 457

 Score = 68.2 bits (165), Expect = 7e-10
 Identities = 45/134 (33%), Positives = 71/134 (52%), Gaps = 6/134 (4%)
 Frame = +2

Query: 38  LSGLENFVGTLQNCFHQIEVLKLLVYT---PEEHIGFEFPK---LHNVKQLSLKIGAWDD 199
           LS  E+F G   + F Q+  LK++V      E  +  +FPK   L N++ L  K   +D 
Sbjct: 281 LSITEDFFGACSSSFSQLMTLKIVVTEVRMQECQVRLKFPKFPELTNLRHLGFKT-EYDI 339

Query: 200 ACLLHFTSLVKACPLLETFMLELLWMSPAKRRRKIRQTANFSHKHLRLVEICGYYGRTTD 379
             +L    L+K+ PLL  F LEL   S  + +R++R   N  H++L++VEI G+ G   +
Sbjct: 340 CSILAVRLLIKSSPLLNKFTLELADGSRCQIKRRVRTGINCPHQNLKVVEIVGFIGGAVE 399

Query: 380 AELALYFIQNAVQL 421
            E  ++F+ NA  L
Sbjct: 400 YEFIMFFLDNASAL 413


>ref|XP_004138258.1| PREDICTED: putative FBD-associated F-box protein At5g56700-like
           [Cucumis sativus] gi|449501454|ref|XP_004161371.1|
           PREDICTED: putative FBD-associated F-box protein
           At5g56700-like [Cucumis sativus]
          Length = 475

 Score = 67.8 bits (164), Expect = 9e-10
 Identities = 39/100 (39%), Positives = 64/100 (64%), Gaps = 1/100 (1%)
 Frame = +2

Query: 125 EHIGF-EFPKLHNVKQLSLKIGAWDDACLLHFTSLVKACPLLETFMLELLWMSPAKRRRK 301
           E+ GF ++P L N+K+L L + A   + L+ FTSL+KA P L  F L+L ++   ++R  
Sbjct: 329 ENQGFRKWPILANLKELKLIVIAEGHSSLIGFTSLIKASPSLLKFTLKLDYLDMFEQR-P 387

Query: 302 IRQTANFSHKHLRLVEICGYYGRTTDAELALYFIQNAVQL 421
           +R+   F H+++++VE+ G+ G+  D EL  YF +NAV L
Sbjct: 388 LRKVKKFPHQYIKVVELAGFVGKPIDLELVQYFHKNAVAL 427


>emb|CAN81846.1| hypothetical protein VITISV_037892 [Vitis vinifera]
          Length = 265

 Score = 58.5 bits (140), Expect = 5e-07
 Identities = 35/95 (36%), Positives = 53/95 (55%), Gaps = 3/95 (3%)
 Frame = +2

Query: 146 PKLHNVKQLSLKIGAWDDACLLHFTSLVKACPLLETFMLELLWMSPAKR---RRKIRQTA 316
           P+L N+KQ  L +    + CLL F +++K  P L+ F+L+       KR   RR +R+  
Sbjct: 124 PELKNLKQFVLNVERRQNDCLLGFIAMIKNWPNLQRFVLQTRHDHDKKRKVARRDLREAI 183

Query: 317 NFSHKHLRLVEICGYYGRTTDAELALYFIQNAVQL 421
             SH++L++VEI GY   + D EL    I+N V L
Sbjct: 184 EDSHQYLKVVEIEGYCNYSYDFELFKQIIENVVTL 218


>ref|XP_002513467.1| ubiquitin-protein ligase, putative [Ricinus communis]
           gi|223547375|gb|EEF48870.1| ubiquitin-protein ligase,
           putative [Ricinus communis]
          Length = 464

 Score = 57.0 bits (136), Expect = 2e-06
 Identities = 36/95 (37%), Positives = 50/95 (52%), Gaps = 2/95 (2%)
 Frame = +2

Query: 143 FPKLHNVKQLSLKIGAWDDA-CLLHFTSLVKACPLLETFMLELLWM-SPAKRRRKIRQTA 316
           FP L NVK L +       A CL    SL+K CPLL  F L+++   S    R   +   
Sbjct: 320 FPILSNVKHLEVTTAVSAVAHCLFPCLSLLKGCPLLHRFTLKVISCESTLVCRASQKVPE 379

Query: 317 NFSHKHLRLVEICGYYGRTTDAELALYFIQNAVQL 421
           + SH  L++VE+ G+ G   + EL LYF++NA  L
Sbjct: 380 DHSHPCLKVVEVLGFQGNAAEVELVLYFLRNAAVL 414


>emb|CBI20286.3| unnamed protein product [Vitis vinifera]
          Length = 402

 Score = 56.2 bits (134), Expect = 3e-06
 Identities = 34/92 (36%), Positives = 53/92 (57%), Gaps = 4/92 (4%)
 Frame = +2

Query: 5   PNLKEVALQQGLSGL-ENFVGTLQNCFHQIEVLKLLV--YTPEEHIGFEFPKLHNVKQLS 175
           P L EV    G  G  +N +  L +C HQ+++L+L V  + PE    +  PKL N+KQL 
Sbjct: 290 PLLVEVLAGVGRLGFAKNMLLKLSSCVHQLQILQLTVDHFFPERSRAYLLPKLRNLKQLK 349

Query: 176 LKIGAW-DDACLLHFTSLVKACPLLETFMLEL 268
           L +     + CLL F +++K CP LE+ +L++
Sbjct: 350 LTVFVGCPENCLLGFVAMLKKCPHLESLVLQV 381