BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00027732
         (825 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002272437.1| PREDICTED: medium-chain-fatty-acid--CoA liga...   400   e-109
ref|XP_002305237.1| acyl:coa ligase acetate-coa synthetase-like ...   388   e-106
ref|XP_002301652.1| acyl:coa ligase acetate-coa synthetase-like ...   387   e-105
ref|XP_002301650.1| predicted protein [Populus trichocarpa] gi|2...   374   e-101
ref|XP_002327560.1| predicted protein [Populus trichocarpa] gi|2...   374   e-101

>ref|XP_002272437.1| PREDICTED: medium-chain-fatty-acid--CoA ligase [Vitis vinifera]
          Length = 588

 Score =  400 bits (1027), Expect = e-109
 Identities = 195/277 (70%), Positives = 236/277 (85%), Gaps = 3/277 (1%)
 Frame = -2

Query: 824  APPPAPLLEKMEHLGFHVMHAYGLTEATGPALVCEWQAKWNILPREQQAKLKARQGVSIL 645
            APPPAPLLEK+E LGFHV+HAYGLTEATGPALVCEWQA+WN LPR+ QAKLKARQG+SIL
Sbjct: 312  APPPAPLLEKIEALGFHVVHAYGLTEATGPALVCEWQAEWNRLPRDHQAKLKARQGISIL 371

Query: 644  TLADVDVKDLDTMKSVPHDGKTMGEIVLRGSSIMKGYFKDKVATEKAFQQGWFLTGDVGV 465
            TLADVDVK+ DTM SVP DGK+MGEIVLRGSSIMKGYFKD  AT KAF+ GWF TGDVGV
Sbjct: 372  TLADVDVKNPDTMASVPRDGKSMGEIVLRGSSIMKGYFKDPEATSKAFKNGWFFTGDVGV 431

Query: 464  VYPDGYLEIKDRSKDVIISGGENISSVELESILYKHHSIIDAAVVAMPHPRWDESPCAFV 285
            ++PDGYLEIKDRSKDVIISGGENISSVE+ESILY+H  +++AAVVAMPHP+W ESPCAF+
Sbjct: 432  IHPDGYLEIKDRSKDVIISGGENISSVEVESILYRHPRVLEAAVVAMPHPKWGESPCAFI 491

Query: 284  VV---RTTDKNTVKEEDIIKHCQENLPKYMVPKKIEFVNEIPKTSTGKPRKNELRATAKT 114
             +   +T   + V E+DII++C++NLP+YM+PKK+E + ++PKT+TGK +K ELR  AKT
Sbjct: 492  TINKNQTGQNDDVSEDDIIQYCRKNLPRYMIPKKVEIMEQLPKTATGKIQKFELRTLAKT 551

Query: 113  LVITEKQTSKKQTNKVNRDRPNYNEHAGGQDQVLAMS 3
            LVI+EK TS K++  VN ++P Y  H    +Q+LA+S
Sbjct: 552  LVISEK-TSNKKSTPVNVEQPKYLAHQ-PHEQILALS 586


>ref|XP_002305237.1| acyl:coa ligase acetate-coa synthetase-like protein [Populus
            trichocarpa] gi|118487892|gb|ABK95768.1| unknown [Populus
            trichocarpa] gi|222848201|gb|EEE85748.1| acyl:coa ligase
            acetate-coa synthetase-like protein [Populus trichocarpa]
          Length = 584

 Score =  388 bits (997), Expect = e-106
 Identities = 191/285 (67%), Positives = 228/285 (80%), Gaps = 11/285 (3%)
 Frame = -2

Query: 824  APPPAPLLEKMEHLGFHVMHAYGLTEATGPALVCEWQAKWNILPREQQAKLKARQGVSIL 645
            APPPA LLEK+E LGFHV HAYGLTEATGPALVCEWQ KWN LP++ Q+KLKARQG+SIL
Sbjct: 303  APPPASLLEKIESLGFHVTHAYGLTEATGPALVCEWQTKWNKLPQQDQSKLKARQGISIL 362

Query: 644  TLADVDVKDLDTMKSVPHDGKTMGEIVLRGSSIMKGYFKDKVATEKAFQQGWFLTGDVGV 465
            TLAD D+KDLDTM S+P DGKTMGEIVLRGSSIMKGYFKD  AT KAF+ GWF+TGDVGV
Sbjct: 363  TLADADIKDLDTMASMPRDGKTMGEIVLRGSSIMKGYFKDPEATSKAFRNGWFVTGDVGV 422

Query: 464  VYPDGYLEIKDRSKDVIISGGENISSVELESILYKHHSIIDAAVVAMPHPRWDESPCAFV 285
            ++PDGYLEIKDRSKDVIISGGENISSVELES+LY+H  +++AAVVAMPHP+W ESPCAF+
Sbjct: 423  IHPDGYLEIKDRSKDVIISGGENISSVELESVLYRHPRVLEAAVVAMPHPKWGESPCAFI 482

Query: 284  VVRTT---DKNTVKEEDIIKHCQENLPKYMVPKKIEFVNEIPKTSTGKPRKNELRATAKT 114
             V+     + N VKE +II +C++NLP++M+PK++EF+ E+PKTSTGK +K +LR  AK 
Sbjct: 483  SVKKNSNGETNDVKESEIIAYCRKNLPRFMIPKRVEFMPELPKTSTGKIQKFQLREVAKN 542

Query: 113  LVITEKQTSKKQT--------NKVNRDRPNYNEHAGGQDQVLAMS 3
              + EK  SKK T         +VN + P Y       +QVLAMS
Sbjct: 543  FAVNEKLPSKKPTGSSTPSVSGQVNTEVPGY-----AHEQVLAMS 582


>ref|XP_002301652.1| acyl:coa ligase acetate-coa synthetase-like protein [Populus
            trichocarpa] gi|222843378|gb|EEE80925.1| acyl:coa ligase
            acetate-coa synthetase-like protein [Populus trichocarpa]
          Length = 586

 Score =  387 bits (994), Expect = e-105
 Identities = 193/282 (68%), Positives = 225/282 (79%), Gaps = 8/282 (2%)
 Frame = -2

Query: 824  APPPAPLLEKMEHLGFHVMHAYGLTEATGPALVCEWQAKWNILPREQQAKLKARQGVSIL 645
            APPPA LL+ +E LGFHV HAYGLTEATGPALVCEWQ KWN LP++ QAKLKARQG+SIL
Sbjct: 303  APPPASLLQDIERLGFHVTHAYGLTEATGPALVCEWQKKWNKLPQQDQAKLKARQGISIL 362

Query: 644  TLADVDVKDLDTMKSVPHDGKTMGEIVLRGSSIMKGYFKDKVATEKAFQQGWFLTGDVGV 465
            TLAD DVKDLDTM SVP DGKTMGEIVLRGSSIMKGYFKD  AT KAF+ GWF TGDVGV
Sbjct: 363  TLADADVKDLDTMVSVPRDGKTMGEIVLRGSSIMKGYFKDPEATSKAFRNGWFATGDVGV 422

Query: 464  VYPDGYLEIKDRSKDVIISGGENISSVELESILYKHHSIIDAAVVAMPHPRWDESPCAFV 285
            ++PDGYLEIKDRSKDVIISGGENISSVELES+LY+H  +++AAVVAMPHP+W ESPCAF+
Sbjct: 423  IHPDGYLEIKDRSKDVIISGGENISSVELESVLYRHPRVLEAAVVAMPHPKWGESPCAFI 482

Query: 284  VVRTT---DKNTVKEEDIIKHCQENLPKYMVPKKIEFVNEIPKTSTGKPRKNELRATAKT 114
             V+     D N VKE DII +C++NLP + VPK++EF+ E+PKTSTGK +K +LRA A+ 
Sbjct: 483  SVKKNSNGDTNDVKESDIIAYCKKNLPHFTVPKRVEFMAELPKTSTGKIQKFQLRALAQN 542

Query: 113  LVITEKQTSKK-----QTNKVNRDRPNYNEHAGGQDQVLAMS 3
             V+ E   SKK     Q +   R      E+A G +QVLA+S
Sbjct: 543  FVVNEILPSKKINGHSQPSASGRVNTEVTEYAQGHEQVLALS 584


>ref|XP_002301650.1| predicted protein [Populus trichocarpa] gi|222843376|gb|EEE80923.1|
           predicted protein [Populus trichocarpa]
          Length = 331

 Score =  374 bits (959), Expect = e-101
 Identities = 180/243 (74%), Positives = 208/243 (85%), Gaps = 3/243 (1%)
 Frame = -2

Query: 824 APPPAPLLEKMEHLGFHVMHAYGLTEATGPALVCEWQAKWNILPREQQAKLKARQGVSIL 645
           APPPA LL+K+E LGFHV HAYGLTEATGPALVCEWQ KWN LP++ QAKLKARQG+SIL
Sbjct: 87  APPPASLLQKIERLGFHVTHAYGLTEATGPALVCEWQKKWNKLPQQDQAKLKARQGISIL 146

Query: 644 TLADVDVKDLDTMKSVPHDGKTMGEIVLRGSSIMKGYFKDKVATEKAFQQGWFLTGDVGV 465
           TLAD DVKDLDTM SVP DGKTMGEIVLRGSSIMKGYFKD  AT KAF+ GW  TGDVGV
Sbjct: 147 TLADADVKDLDTMVSVPRDGKTMGEIVLRGSSIMKGYFKDPEATSKAFRNGWLATGDVGV 206

Query: 464 VYPDGYLEIKDRSKDVIISGGENISSVELESILYKHHSIIDAAVVAMPHPRWDESPCAFV 285
           ++PDGYLEIKDRSKDVIISGGENISSVELES+LY+H  +++AAVVAMPHPRW ESPCAF+
Sbjct: 207 IHPDGYLEIKDRSKDVIISGGENISSVELESVLYRHPRVLEAAVVAMPHPRWGESPCAFI 266

Query: 284 VVR---TTDKNTVKEEDIIKHCQENLPKYMVPKKIEFVNEIPKTSTGKPRKNELRATAKT 114
            V+   + D N VKE DII +C++NLP++ VPK++EF+ E+PKTSTGK  K +LRA A+ 
Sbjct: 267 SVKKNSSGDTNDVKESDIIAYCKKNLPRFTVPKRVEFMAELPKTSTGKVLKIQLRALAQN 326

Query: 113 LVI 105
            V+
Sbjct: 327 FVV 329


>ref|XP_002327560.1| predicted protein [Populus trichocarpa] gi|222836114|gb|EEE74535.1|
            predicted protein [Populus trichocarpa]
          Length = 547

 Score =  374 bits (959), Expect = e-101
 Identities = 180/243 (74%), Positives = 208/243 (85%), Gaps = 3/243 (1%)
 Frame = -2

Query: 824  APPPAPLLEKMEHLGFHVMHAYGLTEATGPALVCEWQAKWNILPREQQAKLKARQGVSIL 645
            APPPA LL+K+E LGFHV HAYGLTEATGPALVCEWQ KWN LP++ QAKLKARQG+SIL
Sbjct: 303  APPPASLLQKIERLGFHVTHAYGLTEATGPALVCEWQKKWNKLPQQDQAKLKARQGISIL 362

Query: 644  TLADVDVKDLDTMKSVPHDGKTMGEIVLRGSSIMKGYFKDKVATEKAFQQGWFLTGDVGV 465
            TLAD DVKDLDTM SVP DGKTMGEIVLRGSSIMKGYFKD  AT KAF+ GW  TGDVGV
Sbjct: 363  TLADADVKDLDTMVSVPRDGKTMGEIVLRGSSIMKGYFKDPEATSKAFRNGWLATGDVGV 422

Query: 464  VYPDGYLEIKDRSKDVIISGGENISSVELESILYKHHSIIDAAVVAMPHPRWDESPCAFV 285
            ++PDGYLEIKDRSKDVIISGGENISSVELES+LY+H  +++AAVVAMPHPRW ESPCAF+
Sbjct: 423  IHPDGYLEIKDRSKDVIISGGENISSVELESVLYRHPRVLEAAVVAMPHPRWGESPCAFI 482

Query: 284  VVR---TTDKNTVKEEDIIKHCQENLPKYMVPKKIEFVNEIPKTSTGKPRKNELRATAKT 114
             V+   + D N VKE DII +C++NLP++ VPK++EF+ E+PKTSTGK  K +LRA A+ 
Sbjct: 483  SVKKNSSGDTNDVKESDIIAYCKKNLPRFTVPKRVEFMAELPKTSTGKVLKIQLRALAQN 542

Query: 113  LVI 105
             V+
Sbjct: 543  FVV 545