BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00027625
         (1961 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002282312.1| PREDICTED: rop guanine nucleotide exchange f...   706   0.0  
ref|XP_002312931.1| predicted protein [Populus trichocarpa] gi|2...   699   0.0  
ref|XP_002528427.1| Rop guanine nucleotide exchange factor, puta...   690   0.0  
ref|XP_002306208.1| predicted protein [Populus trichocarpa] gi|2...   688   0.0  
ref|XP_003540459.1| PREDICTED: rop guanine nucleotide exchange f...   669   0.0  

>ref|XP_002282312.1| PREDICTED: rop guanine nucleotide exchange factor 1 [Vitis vinifera]
            gi|297744497|emb|CBI37759.3| unnamed protein product
            [Vitis vinifera]
          Length = 587

 Score =  706 bits (1823), Expect = 0.0
 Identities = 378/580 (65%), Positives = 425/580 (73%), Gaps = 21/580 (3%)
 Frame = +2

Query: 83   MASVSSE----DCSDRCGIYXXXXXXXXXXXXXXXXXXXXXXXXX---MASPPLARCGLA 241
            M SVSSE    D SDRCG Y                            M S PLA   + 
Sbjct: 1    MGSVSSEEGVDDQSDRCGSYSLSADVSESESCSSFSCRRYDGEGASSSMTSSPLACRPVV 60

Query: 242  GNLCFPA--PVMVPAVGREGAMAWEKKIENPQSDLTEVEMIKERFAKLLLGEDMSGGGKG 415
            GN  FP   P+M+P +G    + W +K E  ++DL+E EM+KERFAKLLLGEDMSGGGKG
Sbjct: 61   GNSSFPVGPPLMLPVIGGRDVVVWGEKPEKRETDLSEAEMMKERFAKLLLGEDMSGGGKG 120

Query: 416  VCTALAISNAITNLSATVFGELWRLEPLAQQKKAMWCREMEYLLCVSDSIVELVPSIQQF 595
            VCTALAISNAITNLSATVFGELWRLEPLA QKKAMWCREME+LLCVSDSIVELVPSIQQF
Sbjct: 121  VCTALAISNAITNLSATVFGELWRLEPLAPQKKAMWCREMEWLLCVSDSIVELVPSIQQF 180

Query: 596  PGGGSYEVMATQPRSDLYINLPALKKLDAMLISILDGFHDLEFSYVDRGMVVADGNDCVA 775
            PGGG+YEVMAT+PRSDLY+NLPALKKLDAML+S+LDGF + EF YVDRG++VA+ ++  A
Sbjct: 181  PGGGTYEVMATRPRSDLYMNLPALKKLDAMLLSMLDGFCETEFWYVDRGIIVAEADNHDA 240

Query: 776  YPSSFSSGRPSVRQEEKWWLPYPKVPPKGLSEESRKRLQQSRECTNQILKAALAINSNVL 955
            YP S SSGRPS+RQEEKWWLP PKVPP GLSE++RKRLQQ R+CTNQILKAA+AINS+VL
Sbjct: 241  YPLSASSGRPSIRQEEKWWLPCPKVPPNGLSEDARKRLQQCRDCTNQILKAAMAINSSVL 300

Query: 956  AEMEIPSSYLHTLPKNGKACLGDIIYCYITGDQFXXXXXXXXXXXXXXHQTLEVANRIEA 1135
            AEMEIP++YL TLPKNGKACLGDIIY YIT +QF              H TLE+ANRIEA
Sbjct: 301  AEMEIPTAYLETLPKNGKACLGDIIYRYITAEQFSPECLLDCLDLSSEHHTLEIANRIEA 360

Query: 1136 AVHIWKRKDQXXXXXXXXXXXXXXXGKVKGLVVDGDKNSFLAQRAETLLHGLKLRFPGLP 1315
            AVH+WK+KD                GKVKGLV D +KN FLA RAETLLH L+LRFPGLP
Sbjct: 361  AVHVWKQKDWKKQPKHLKAKRSTWGGKVKGLVADTEKNQFLAIRAETLLHSLRLRFPGLP 420

Query: 1316 QTALDMHKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDATKRC-SAESVSFL 1492
            QTALDM+KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDA KRC +AES++  
Sbjct: 421  QTALDMNKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAVKRCAAAESMALF 480

Query: 1493 SRRNLIGHPIQKRMSPSPFSIEHTPYASPFATPLFSSSPPIGESSGRITMPINNNGLKG- 1669
             R  L G PIQKRMSPSPFSI+H+P+ASPFATP F SS P+  S GR     N    KG 
Sbjct: 481  GRGGLGGLPIQKRMSPSPFSIQHSPFASPFATPTFCSSTPLTGSPGRTPPSRNKMSSKGA 540

Query: 1670 ----------VPSHML*IPITADLENLWSDAGNLSTRRIS 1759
                       P   L  PI AD E +WS  GNLS R++S
Sbjct: 541  PELKLEKPIPAPEMKLEKPIPADFEKMWSYTGNLSARKVS 580


>ref|XP_002312931.1| predicted protein [Populus trichocarpa] gi|222849339|gb|EEE86886.1|
            predicted protein [Populus trichocarpa]
          Length = 577

 Score =  699 bits (1803), Expect = 0.0
 Identities = 351/510 (68%), Positives = 404/510 (79%), Gaps = 2/510 (0%)
 Frame = +2

Query: 236  LAGNLCFPAPVMVPAVGREGAMAWEKKIENP-QSDLTEVEMIKERFAKLLLGEDMSGGGK 412
            +AG  CFPAPVMVP +G +  + W  K E   ++DL+EVEM+KERFAKLLLGEDMSGGGK
Sbjct: 61   VAGGFCFPAPVMVPVIGGKDVVVWGSKNEKRRETDLSEVEMMKERFAKLLLGEDMSGGGK 120

Query: 413  GVCTALAISNAITNLSATVFGELWRLEPLAQQKKAMWCREMEYLLCVSDSIVELVPSIQQ 592
            GVCTALAISNAITNLSATVFGELWRLEPL  QKK+MW REME+LLCVSDSIVELVPS+QQ
Sbjct: 121  GVCTALAISNAITNLSATVFGELWRLEPLLPQKKSMWKREMEWLLCVSDSIVELVPSMQQ 180

Query: 593  FPGGGSYEVMATQPRSDLYINLPALKKLDAMLISILDGFHDLEFSYVDRGMVVADGNDCV 772
            FPGGG+YEVMAT+PRSDLY+NLPALKKLDAMLIS+LDGF + EF YVDRG+VVADG DC 
Sbjct: 181  FPGGGTYEVMATRPRSDLYVNLPALKKLDAMLISMLDGFCETEFWYVDRGIVVADGGDCD 240

Query: 773  AYPSSFSSGRPSVRQEEKWWLPYPKVPPKGLSEESRKRLQQSRECTNQILKAALAINSNV 952
            AYPS  S GRPS+RQEEKWWLP PKVPP GLS+++RKRLQQ R+CTNQILKAA+AINS+V
Sbjct: 241  AYPSGISGGRPSIRQEEKWWLPCPKVPPNGLSDDARKRLQQCRDCTNQILKAAMAINSSV 300

Query: 953  LAEMEIPSSYLHTLPKNGKACLGDIIYCYITGDQFXXXXXXXXXXXXXXHQTLEVANRIE 1132
            L EMEIP++Y+ TLPKNGKACLGDIIY YIT +QF              H TLE+ANRIE
Sbjct: 301  LVEMEIPTAYMETLPKNGKACLGDIIYRYITAEQFSPDRLLDCLDLSTEHHTLEIANRIE 360

Query: 1133 AAVHIWKRKDQXXXXXXXXXXXXXXXGKVKGLVVDGDKNSFLAQRAETLLHGLKLRFPGL 1312
            AAVH+WK+KD                GKVKG V D  KN  LAQRAETLL  L+LRFPGL
Sbjct: 361  AAVHVWKQKDHKKHTHNMRIKHSSWGGKVKGFVTDSHKNQILAQRAETLLQSLRLRFPGL 420

Query: 1313 PQTALDMHKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDATKRC-SAESVSF 1489
            PQT LDM+KIQYNKDVGQSILESYSRVMESLAFNIMARIDDV+YVDDA K+C +AES+S 
Sbjct: 421  PQTTLDMNKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVIYVDDAIKQCAAAESMSL 480

Query: 1490 LSRRNLIGHPIQKRMSPSPFSIEHTPYASPFATPLFSSSPPIGESSGRITMPINNNGLKG 1669
             +R  L G PIQKRMSPSPFSI+ +P+ASPFATP + +S P+  S  R+   +     K 
Sbjct: 481  FNRGGLGGLPIQKRMSPSPFSIQQSPFASPFATPTYCASTPVSGSPERVPSSLKRGKPKE 540

Query: 1670 VPSHML*IPITADLENLWSDAGNLSTRRIS 1759
             P   L  P+++D E +W+  GNLS+RR+S
Sbjct: 541  APDRKLEKPLSSDFETVWTYTGNLSSRRVS 570


>ref|XP_002528427.1| Rop guanine nucleotide exchange factor, putative [Ricinus communis]
            gi|223532163|gb|EEF33969.1| Rop guanine nucleotide
            exchange factor, putative [Ricinus communis]
          Length = 580

 Score =  690 bits (1781), Expect = 0.0
 Identities = 354/519 (68%), Positives = 405/519 (78%), Gaps = 2/519 (0%)
 Frame = +2

Query: 209  ASPPLARCGLAGNLCFPAPVMVPAVGREGAMAWEKKIENPQSDLTEVEMIKERFAKLLLG 388
            +SPPL     +   CF  P+++P    +  + W+ K E   +DL+EVEM+KERFAKLLLG
Sbjct: 62   SSPPLPS---SAKFCFQTPLLLP----KDLLLWDIKPEKRDTDLSEVEMMKERFAKLLLG 114

Query: 389  EDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLAQQKKAMWCREMEYLLCVSDSIV 568
            EDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLA QKK+MW REME+LLCVSDSIV
Sbjct: 115  EDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLAPQKKSMWRREMEWLLCVSDSIV 174

Query: 569  ELVPSIQQFPGGGSYEVMATQPRSDLYINLPALKKLDAMLISILDGFHDLEFSYVDRGMV 748
            ELVPS+QQFPGGG+YE+MA QPRSDLY+NLPALKKLDAMLIS+LDGF + EF YVDRG++
Sbjct: 175  ELVPSVQQFPGGGTYEIMAAQPRSDLYVNLPALKKLDAMLISMLDGFCETEFWYVDRGII 234

Query: 749  VADGNDCVAYPSSFSSGRPSVRQEEKWWLPYPKVPPKGLSEESRKRLQQSRECTNQILKA 928
            +ADG D   YPS FS+GRPS+RQEEKWWLP PK+P  GLSE++RKRLQQ R+CTNQILKA
Sbjct: 235  LADGADNDVYPSGFSTGRPSIRQEEKWWLPCPKLPSNGLSEDARKRLQQCRDCTNQILKA 294

Query: 929  ALAINSNVLAEMEIPSSYLHTLPKNGKACLGDIIYCYITGDQFXXXXXXXXXXXXXXHQT 1108
            A+AINS+VLAEMEIP++YL TLPK GKACLGDIIY YIT +QF              H T
Sbjct: 295  AMAINSSVLAEMEIPTAYLETLPKCGKACLGDIIYRYITAEQFSPECLLDCLDLSTEHHT 354

Query: 1109 LEVANRIEAAVHIWKRKDQXXXXXXXXXXXXXXXGKVKGLVVDGD-KNSFLAQRAETLLH 1285
            LE+ANRIEAA+H+WK+KDQ               GKVKGLV D   KN+FLAQRAETLL 
Sbjct: 355  LEIANRIEAALHVWKQKDQRKHTSHTKARHTSWGGKVKGLVADTKRKNTFLAQRAETLLQ 414

Query: 1286 GLKLRFPGLPQTALDMHKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDATKR 1465
             L+LRFPGLPQTALDMHKIQYNKDVGQSILESYSRVMESLAFNIMARIDDV+YVDDAT R
Sbjct: 415  SLRLRFPGLPQTALDMHKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVIYVDDATNR 474

Query: 1466 C-SAESVSFLSRRNLIGHPIQKRMSPSPFSIEHTPYASPFATPLFSSSPPIGESSGRITM 1642
            C +AES+S  +R  L GHPIQKRMSPSPFSI+ +P+ SPFATP F SS P+  S GR   
Sbjct: 475  CAAAESMSLFNRSGLGGHPIQKRMSPSPFSIQQSPFTSPFATPTFCSSTPVAGSPGRAPS 534

Query: 1643 PINNNGLKGVPSHML*IPITADLENLWSDAGNLSTRRIS 1759
             I  N +K         P  AD E +WS  GNLS+RR+S
Sbjct: 535  SIKMNNIKEATDQKFEKPCPADFEKVWSYTGNLSSRRVS 573


>ref|XP_002306208.1| predicted protein [Populus trichocarpa] gi|222849172|gb|EEE86719.1|
            predicted protein [Populus trichocarpa]
          Length = 576

 Score =  688 bits (1775), Expect = 0.0
 Identities = 346/510 (67%), Positives = 400/510 (78%), Gaps = 2/510 (0%)
 Frame = +2

Query: 236  LAGNLCFPAPVMVPAVGREGAMAWEKKIENP-QSDLTEVEMIKERFAKLLLGEDMSGGGK 412
            L G  CFPAPVMVP +G +  + W+ K E    +DL+EVEM+KERFAKLLLGEDMSGGGK
Sbjct: 61   LPGGFCFPAPVMVPVIGGKDVVVWDSKNEKRGDADLSEVEMMKERFAKLLLGEDMSGGGK 120

Query: 413  GVCTALAISNAITNLSATVFGELWRLEPLAQQKKAMWCREMEYLLCVSDSIVELVPSIQQ 592
            GVCTALAISNAITNLSATVFGELWRLEPLA QKK+MW REME+LLCVSDSIVELVPSIQQ
Sbjct: 121  GVCTALAISNAITNLSATVFGELWRLEPLALQKKSMWKREMEWLLCVSDSIVELVPSIQQ 180

Query: 593  FPGGGSYEVMATQPRSDLYINLPALKKLDAMLISILDGFHDLEFSYVDRGMVVADGNDCV 772
            FPGGG+YEVMAT+PRSDLY+NLPALKKLDAMLI++LDGF + EF Y DRG+VV DG DC 
Sbjct: 181  FPGGGTYEVMATRPRSDLYVNLPALKKLDAMLITMLDGFSETEFWYADRGIVVGDGGDCD 240

Query: 773  AYPSSFSSGRPSVRQEEKWWLPYPKVPPKGLSEESRKRLQQSRECTNQILKAALAINSNV 952
             YPS  S GRPS+RQE+KWWLP PKVPP GLSE++RKRLQQ R+CTNQILKAA+AINS+V
Sbjct: 241  TYPSGISGGRPSIRQEDKWWLPCPKVPPDGLSEDARKRLQQCRDCTNQILKAAMAINSSV 300

Query: 953  LAEMEIPSSYLHTLPKNGKACLGDIIYCYITGDQFXXXXXXXXXXXXXXHQTLEVANRIE 1132
            L EMEIP++Y+ TLPKNGKACLGDIIY YIT ++F              H TLE+ANR+E
Sbjct: 301  LVEMEIPTAYMETLPKNGKACLGDIIYRYITAEKFSPECLLDCLDLSTEHHTLEIANRVE 360

Query: 1133 AAVHIWKRKDQXXXXXXXXXXXXXXXGKVKGLVVDGDKNSFLAQRAETLLHGLKLRFPGL 1312
            AAVHIWK+KD                GKVKG V D  KN  LAQRAETLL  L+LR+PGL
Sbjct: 361  AAVHIWKQKDHRKHIHNKNIKHSSWGGKVKGFVADTHKNQILAQRAETLLQSLRLRYPGL 420

Query: 1313 PQTALDMHKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDATKRC-SAESVSF 1489
            PQTALDM+KIQYNKDVGQSILESYSRV ESLAFNIMARIDDVL+VDDATK+C +AES+S 
Sbjct: 421  PQTALDMNKIQYNKDVGQSILESYSRVTESLAFNIMARIDDVLFVDDATKQCAAAESISL 480

Query: 1490 LSRRNLIGHPIQKRMSPSPFSIEHTPYASPFATPLFSSSPPIGESSGRITMPINNNGLKG 1669
             +R  L GHPIQKRMSPSPFSI+ +P+ASP+ TP + +S P+  S  R+  P      K 
Sbjct: 481  FNRGGLGGHPIQKRMSPSPFSIKQSPFASPYGTPTYFASTPVSGSPARVP-PQKRGNPKE 539

Query: 1670 VPSHML*IPITADLENLWSDAGNLSTRRIS 1759
                    P++ D E +WS AG++S+RR+S
Sbjct: 540  ASDQKFEKPLSPDFERVWSYAGSISSRRVS 569


>ref|XP_003540459.1| PREDICTED: rop guanine nucleotide exchange factor 1-like isoform 1
            [Glycine max]
          Length = 562

 Score =  669 bits (1726), Expect = 0.0
 Identities = 364/566 (64%), Positives = 413/566 (72%), Gaps = 7/566 (1%)
 Frame = +2

Query: 83   MASVSSEDCSDRCGIYXXXXXXXXXXXXXXXXXXXXXXXXXMASPPLARCGLAGNLCFP- 259
            M SVSSED SDRCG Y                          +S  L+   +A +  FP 
Sbjct: 1    MGSVSSEDGSDRCGSYSLSADVSESESCSSFSARRFDAEGASSSANLSPRPVAAHFNFPP 60

Query: 260  APVMVPAVGREGAMAWEKKIENPQSDLTEVEMIKERFAKLLLGEDMSGGGKGVCTALAIS 439
            A VM+P +G +  + W+ K +    DL+EVEM+KERFAKLLLGEDMSGGGKGVCTALAIS
Sbjct: 61   AQVMLPVIGGKDVVVWDHKRD---LDLSEVEMMKERFAKLLLGEDMSGGGKGVCTALAIS 117

Query: 440  NAITNLSATVFGELWRLEPLAQQKKAMWCREMEYLLCVSDSIVELVPSIQQFPGGGSYEV 619
            NAITNLSATVFGELWRLEPLA QKK MW REME+LLCVSDSIVELVPS+QQFPGGG+YEV
Sbjct: 118  NAITNLSATVFGELWRLEPLAPQKKTMWRREMEWLLCVSDSIVELVPSVQQFPGGGTYEV 177

Query: 620  MATQPRSDLYINLPALKKLDAMLISILDGFHDLEFSYVDRGMVVADGNDCVAYPSSFSSG 799
            MAT+PRSDLYINLPALKKLD ML+++LDGFHD +F YVDRG+++ D  DC AY      G
Sbjct: 178  MATRPRSDLYINLPALKKLDGMLLNMLDGFHDTQFWYVDRGIILGDSKDCDAY------G 231

Query: 800  RPSVRQEEKWWLPYPKVPPKGLSEESRKRLQQSRECTNQILKAALAINSNVLAEMEIPSS 979
            RPSVRQEEKWWLP PK+PP GLSEESRKRLQQ R+CTNQILKAA+AIN++VLAEMEIP +
Sbjct: 232  RPSVRQEEKWWLPSPKLPPNGLSEESRKRLQQCRDCTNQILKAAVAINTSVLAEMEIPGA 291

Query: 980  YLHTLPKNGKACLGDIIYCYITGDQFXXXXXXXXXXXXXXHQTLEVANRIEAAVHIWKRK 1159
            Y+ +LPKNGKACLGDIIY YIT DQF              H TL++ANRIEAA+H+W+ K
Sbjct: 292  YIESLPKNGKACLGDIIYRYITADQFSPECLLDCLDLSTEHHTLDIANRIEAAIHVWRLK 351

Query: 1160 DQXXXXXXXXXXXXXXXGKVKGLVVDGD--KNSFLAQRAETLLHGLKLRFPGLPQTALDM 1333
            D                GKVKGLV DG+  KN+FLAQRAETLL  LK RFPGLPQTALDM
Sbjct: 352  DH-KKHLSSAKSRRSWGGKVKGLVADGEKNKNNFLAQRAETLLESLKHRFPGLPQTALDM 410

Query: 1334 HKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDATKRC-SAESVSFLSRRNLI 1510
             KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDD+ KRC +A+S+S  SR    
Sbjct: 411  AKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDSIKRCAAADSLSLFSRGGFG 470

Query: 1511 GHPIQKRMSPSPFSIEHTPYASPFATPLFSSSPPIGE---SSGRITMPINNNGLKGVPSH 1681
            G PIQKR SPSPFSI+HTPYASPFATP F SS P+     S  RI     N   +G  S 
Sbjct: 471  GMPIQKRFSPSPFSIQHTPYASPFATPTFCSSTPVTGSPCSPARIHDVKRNAPKEGADSK 530

Query: 1682 ML*IPITADLENLWSDAGNLSTRRIS 1759
               +  T++ E +WS AGNLS RR S
Sbjct: 531  TEKL-ATSEFERVWSYAGNLSARRAS 555