BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00027563
         (1729 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002280567.1| PREDICTED: GPI mannosyltransferase 1 [Vitis ...   643   0.0  
ref|XP_003531463.1| PREDICTED: GPI mannosyltransferase 1-like [G...   622   e-176
ref|XP_003627964.1| GPI mannosyltransferase [Medicago truncatula...   617   e-174
ref|NP_680199.1| GPI mannosyltransferase 1 [Arabidopsis thaliana...   612   e-172
gb|AAP59446.1| PEANUT1 [Arabidopsis thaliana]                         612   e-172

>ref|XP_002280567.1| PREDICTED: GPI mannosyltransferase 1 [Vitis vinifera]
          Length = 438

 Score =  643 bits (1659), Expect = 0.0
 Identities = 304/424 (71%), Positives = 351/424 (82%), Gaps = 1/424 (0%)
 Frame = -3

Query: 1595 INFRSIILFSTFLRFFLIIYGEWQDTHLEVRYTDVDYLVFSDAAALVVSGHSPYKRSTYR 1416
            ++ RS++LFS  LR  LIIYGEWQD H+EVRYTDVDYLVFSDAA+LV SG SPY+RSTYR
Sbjct: 1    MDVRSLLLFSALLRVLLIIYGEWQDAHMEVRYTDVDYLVFSDAASLVASGKSPYQRSTYR 60

Query: 1415 YSPLIAYLLTPNSFIHRSWGKVIFSVSDLLVGLFIRSILKLQGVSENMCIYSVMVWLFNP 1236
            YSPL+A+LL PNS IH SWGK +FS SDLLVGLFI +IL+L+ V EN+C  ++MVWLFNP
Sbjct: 61   YSPLLAFLLVPNSIIHPSWGKFLFSASDLLVGLFIHTILELRKVPENLCTCALMVWLFNP 120

Query: 1235 FTFTIGTRGNCEPIICAMVLWIILCLMKGKLLQAAFWYGLVVHLRIYPIIYALPIVLALD 1056
            FTF+IGTRGNCEPI+CAM+LWII+CLM GK+LQAAFWYGLVVH RIYPIIY++PI+L LD
Sbjct: 121  FTFSIGTRGNCEPIVCAMILWIIICLMNGKVLQAAFWYGLVVHFRIYPIIYSIPIILVLD 180

Query: 1055 PCHFQYGKKPVLRKWTSSDMNSVQSSTTKIVSEKVHILVVIPRLFTKERILFGLLSGTVF 876
            P  F YG KP L  W     NS QS  +  V E   I + +  +FT+ RI+FGL+SG VF
Sbjct: 181  PHCFLYGLKPALSSWVGQG-NSTQSIPSTAVPESCGIWIALTSMFTRARIMFGLISGAVF 239

Query: 875  FFLTGIFFYLYELEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYEEKFSLIEKLVSFLPQ 696
            F  TG+FFYLY  EFLHEALLYHLTRTDPRHNFSIYFYHIYLHYE +FS++E+L+SFLPQ
Sbjct: 240  FAFTGLFFYLYRWEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYEHEFSVVERLISFLPQ 299

Query: 695  FLVQIVLIYRFQKDLPFCFFLQTVAFVAFNKVITAQYFVWFFCLLPLILPWSTMKLKW-G 519
            FLVQ+VLI  F +DLPFCFF QTVAFVAFNKVITAQYFVWFFCLLPLILPWS MKLKW G
Sbjct: 300  FLVQLVLIVCFAQDLPFCFFAQTVAFVAFNKVITAQYFVWFFCLLPLILPWSNMKLKWTG 359

Query: 518  LICIFLWVGAQTHWLLWGYLLEFKGKQVFLFLWVAGLLFLFVNTFVIICLIIHQNCSSLF 339
            L CI LW+G QTHWL+WGYLLEFKGK VFL LW+A +LFL  NTFV+I +I +   SSLF
Sbjct: 360  LSCILLWMGTQTHWLMWGYLLEFKGKNVFLELWIASILFLAANTFVLIKIIRYHTYSSLF 419

Query: 338  KPLE 327
            + LE
Sbjct: 420  QRLE 423


>ref|XP_003531463.1| PREDICTED: GPI mannosyltransferase 1-like [Glycine max]
          Length = 433

 Score =  622 bits (1605), Expect = e-176
 Identities = 294/427 (68%), Positives = 345/427 (80%), Gaps = 1/427 (0%)
 Frame = -3

Query: 1604 MGLINFRSIILFSTFLRFFLIIYGEWQDTHLEVRYTDVDYLVFSDAAALVVSGHSPYKRS 1425
            M  ++  S++LFS   R  LI+YGEWQD H+EVRYTDVDYLVFSDAAAL+  G SPYKR+
Sbjct: 1    MASLDIHSLLLFSAIFRVILIVYGEWQDAHMEVRYTDVDYLVFSDAAALMAVGDSPYKRT 60

Query: 1424 TYRYSPLIAYLLTPNSFIHRSWGKVIFSVSDLLVGLFIRSILKLQGVSENMCIYSVMVWL 1245
            TYRYSPL+A+LL PNSFIHRSWGK +FS SDLLVG FI  ILKL+ V EN+C YSVM WL
Sbjct: 61   TYRYSPLLAFLLIPNSFIHRSWGKFLFSASDLLVGYFIYYILKLRKVPENLCNYSVMTWL 120

Query: 1244 FNPFTFTIGTRGNCEPIICAMVLWIILCLMKGKLLQAAFWYGLVVHLRIYPIIYALPIVL 1065
            FNPFTFTIGTRGNCEPI+CA+VLWII+CLMKG +LQ+AFWYGLVVH RIYPIIY++PI+L
Sbjct: 121  FNPFTFTIGTRGNCEPIVCAVVLWIIICLMKGNVLQSAFWYGLVVHFRIYPIIYSIPIIL 180

Query: 1064 ALDPCHFQYGKKPVLRKWTSSDMNSVQSSTTKIVSEKVHILVVIPRLFTKERILFGLLSG 885
             LDP  F   +KPVLR W     ++VQ    K    +  +  +   +FT+ R++FGL+SG
Sbjct: 181  VLDPNFFPSNQKPVLRNW-----SAVQGERPKDGCGQHTLHNLFKNIFTRNRLIFGLVSG 235

Query: 884  TVFFFLTGIFFYLYELEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYEEKFSLIEKLVSF 705
             VF F TG+FF LYE EFLHEALLYHLTRTDPRHNFSIYFYHIYLHY +  S++EKL+SF
Sbjct: 236  LVFLFCTGLFFCLYEWEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYGQDTSVVEKLISF 295

Query: 704  LPQFLVQIVLIYRFQKDLPFCFFLQTVAFVAFNKVITAQYFVWFFCLLPLILPWSTMKLK 525
            LPQF VQ+VLI+ F +DL FC F+QTVAFVAFNKVITAQYFVWFFCLLPLILPWS MKL+
Sbjct: 296  LPQFFVQLVLIFCFAQDLTFCLFVQTVAFVAFNKVITAQYFVWFFCLLPLILPWSKMKLR 355

Query: 524  W-GLICIFLWVGAQTHWLLWGYLLEFKGKQVFLFLWVAGLLFLFVNTFVIICLIIHQNCS 348
            W GL CI +W+GAQTHWL+WGYLLEFKGK VFL LW AGLLFL  N F+++ +I H   +
Sbjct: 356  WDGLSCILVWIGAQTHWLMWGYLLEFKGKNVFLQLWAAGLLFLAANIFILVMIIRHHKYA 415

Query: 347  SLFKPLE 327
            S+FK LE
Sbjct: 416  SVFKALE 422


>ref|XP_003627964.1| GPI mannosyltransferase [Medicago truncatula]
            gi|355521986|gb|AET02440.1| GPI mannosyltransferase
            [Medicago truncatula]
          Length = 433

 Score =  617 bits (1591), Expect = e-174
 Identities = 290/428 (67%), Positives = 340/428 (79%), Gaps = 1/428 (0%)
 Frame = -3

Query: 1604 MGLINFRSIILFSTFLRFFLIIYGEWQDTHLEVRYTDVDYLVFSDAAALVVSGHSPYKRS 1425
            M  ++ RS++ FS   R  LI+YGEWQD H+EVRYTDVDY+VFSDA++LV SG+SPY+R+
Sbjct: 1    MAWLDIRSLLTFSAIFRVILILYGEWQDAHMEVRYTDVDYIVFSDASSLVASGYSPYQRT 60

Query: 1424 TYRYSPLIAYLLTPNSFIHRSWGKVIFSVSDLLVGLFIRSILKLQGVSENMCIYSVMVWL 1245
            TYRYSPL+A+LL PN FIH SWGK +FS +D+LVG FI  ILKLQ V EN+C YSVM WL
Sbjct: 61   TYRYSPLLAFLLLPNHFIHHSWGKFLFSSADILVGYFIYYILKLQKVPENLCNYSVMAWL 120

Query: 1244 FNPFTFTIGTRGNCEPIICAMVLWIILCLMKGKLLQAAFWYGLVVHLRIYPIIYALPIVL 1065
             NPFTFTIGTRGNCEP++ AM+LWII+CLMKG +LQ+AFWYGLVVH RIYPIIY++PI+L
Sbjct: 121  LNPFTFTIGTRGNCEPVVSAMLLWIIVCLMKGNVLQSAFWYGLVVHFRIYPIIYSIPIIL 180

Query: 1064 ALDPCHFQYGKKPVLRKWTSSDMNSVQSSTTKIVSEKVHILVVIPRLFTKERILFGLLSG 885
             LDP  F  G+KPVLR W++      +             L  +  LFT  R++FGL+SG
Sbjct: 181  VLDPNFFPSGQKPVLRNWSTFQKERPEDRNGLFTP-----LNFLKSLFTWNRMMFGLVSG 235

Query: 884  TVFFFLTGIFFYLYELEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYEEKFSLIEKLVSF 705
             VF   TG+F+ LY  EFLHEALLYHLTRTDPRHNFSIYFYHIYLHY    S++EKLVSF
Sbjct: 236  LVFLVCTGLFYCLYGWEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYGNDISVVEKLVSF 295

Query: 704  LPQFLVQIVLIYRFQKDLPFCFFLQTVAFVAFNKVITAQYFVWFFCLLPLILPWSTMKLK 525
            LPQF VQ+VLI+ F +DLPFC F+QTV+FVAFNKVITAQYFVWFFCLLPLILPWS MKLK
Sbjct: 296  LPQFFVQLVLIFSFARDLPFCLFVQTVSFVAFNKVITAQYFVWFFCLLPLILPWSKMKLK 355

Query: 524  W-GLICIFLWVGAQTHWLLWGYLLEFKGKQVFLFLWVAGLLFLFVNTFVIICLIIHQNCS 348
            W GL CI LW+GAQTHWLLWGYLLEFKGK VFL LW AGLLFL  N F+++ +I   NCS
Sbjct: 356  WGGLSCILLWIGAQTHWLLWGYLLEFKGKNVFLQLWAAGLLFLAANIFILVMIICQHNCS 415

Query: 347  SLFKPLEN 324
            S+FK LEN
Sbjct: 416  SVFKALEN 423


>ref|NP_680199.1| GPI mannosyltransferase 1 [Arabidopsis thaliana]
            gi|75319819|sp|Q500W7.1|PIGM_ARATH RecName: Full=GPI
            mannosyltransferase 1; AltName: Full=GPI
            mannosyltransferase I; Short=GPI-MT-I; AltName:
            Full=Phosphatidylinositol-glycan biosynthesis class M
            protein; Short=PIG-M; AltName: Full=Protein PEANUT 1
            gi|63147376|gb|AAY34161.1| At5g22130 [Arabidopsis
            thaliana] gi|110741556|dbj|BAE98727.1|
            mannosyltransferase-like protein [Arabidopsis thaliana]
            gi|111074510|gb|ABH04628.1| At5g22130 [Arabidopsis
            thaliana] gi|332005603|gb|AED92986.1| GPI
            mannosyltransferase 1 [Arabidopsis thaliana]
          Length = 450

 Score =  612 bits (1577), Expect = e-172
 Identities = 288/425 (67%), Positives = 339/425 (79%), Gaps = 1/425 (0%)
 Frame = -3

Query: 1595 INFRSIILFSTFLRFFLIIYGEWQDTHLEVRYTDVDYLVFSDAAALVVSGHSPYKRSTYR 1416
            + FRS+++FS  LR FLI+YGEWQD H+EVRYTDVDY+VFSDAA+L+ SG SPYKR+TYR
Sbjct: 16   MKFRSLLVFSMLLRVFLIVYGEWQDAHMEVRYTDVDYIVFSDAASLMASGESPYKRTTYR 75

Query: 1415 YSPLIAYLLTPNSFIHRSWGKVIFSVSDLLVGLFIRSILKLQGVSENMCIYSVMVWLFNP 1236
            YSPL+A LLTPNSF HRSWGK +FS SDLLVG FI  ILK + V E +C YSVMVWLFNP
Sbjct: 76   YSPLLALLLTPNSFFHRSWGKFLFSASDLLVGWFIHKILKQRKVPEKICTYSVMVWLFNP 135

Query: 1235 FTFTIGTRGNCEPIICAMVLWIILCLMKGKLLQAAFWYGLVVHLRIYPIIYALPIVLALD 1056
            FTFTIGTRGNCEPI+CAM+LWIILCLM+G LLQAAFWYGLVVH R+YPIIYALPI+L LD
Sbjct: 136  FTFTIGTRGNCEPIVCAMILWIILCLMQGNLLQAAFWYGLVVHFRVYPIIYALPIILVLD 195

Query: 1055 PCHFQYGKKPVLRKWTSSDMNSVQSSTTKIVSEKVHILVVIPRLFTKERILFGLLSGTVF 876
               F+ G+KP L  W +    +  S+  +  +   ++L  +  LF++ERI+F L+SG VF
Sbjct: 196  TQVFRSGQKPALLYWNTGQAKTPASNMER-KTFLFNLLTTLKSLFSRERIMFALISGGVF 254

Query: 875  FFLTGIFFYLYELEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYEEKFSLIEKLVSFLPQ 696
                 + FY Y  EFLHEALLYHLTRTDPRHNFSIYFYHIYLHYE +FS +EKL+SFLPQ
Sbjct: 255  LACNAVSFYFYGQEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYERQFSAVEKLISFLPQ 314

Query: 695  FLVQIVLIYRFQKDLPFCFFLQTVAFVAFNKVITAQYFVWFFCLLPLILPWSTMKLKW-G 519
            F VQ  L++ F +DL FC FLQTVAFV FNKVITAQYFVWF+CLLPLILPWS MKLKW G
Sbjct: 315  FTVQFALVFCFSQDLVFCIFLQTVAFVTFNKVITAQYFVWFYCLLPLILPWSHMKLKWEG 374

Query: 518  LICIFLWVGAQTHWLLWGYLLEFKGKQVFLFLWVAGLLFLFVNTFVIICLIIHQNCSSLF 339
            L+CI +W+GAQTHWLLWGY+LEFKG  VFL LW+A LLFL  NTFV++ +I     S LF
Sbjct: 375  LLCIIMWIGAQTHWLLWGYMLEFKGVNVFLPLWIASLLFLAANTFVLVRIIQRHRFSPLF 434

Query: 338  KPLEN 324
            +  E+
Sbjct: 435  RRYES 439


>gb|AAP59446.1| PEANUT1 [Arabidopsis thaliana]
          Length = 435

 Score =  612 bits (1577), Expect = e-172
 Identities = 288/425 (67%), Positives = 339/425 (79%), Gaps = 1/425 (0%)
 Frame = -3

Query: 1595 INFRSIILFSTFLRFFLIIYGEWQDTHLEVRYTDVDYLVFSDAAALVVSGHSPYKRSTYR 1416
            + FRS+++FS  LR FLI+YGEWQD H+EVRYTDVDY+VFSDAA+L+ SG SPYKR+TYR
Sbjct: 1    MKFRSLLVFSMLLRVFLIVYGEWQDAHMEVRYTDVDYIVFSDAASLMASGESPYKRTTYR 60

Query: 1415 YSPLIAYLLTPNSFIHRSWGKVIFSVSDLLVGLFIRSILKLQGVSENMCIYSVMVWLFNP 1236
            YSPL+A LLTPNSF HRSWGK +FS SDLLVG FI  ILK + V E +C YSVMVWLFNP
Sbjct: 61   YSPLLALLLTPNSFFHRSWGKFLFSASDLLVGWFIHKILKQRKVPEKICTYSVMVWLFNP 120

Query: 1235 FTFTIGTRGNCEPIICAMVLWIILCLMKGKLLQAAFWYGLVVHLRIYPIIYALPIVLALD 1056
            FTFTIGTRGNCEPI+CAM+LWIILCLM+G LLQAAFWYGLVVH R+YPIIYALPI+L LD
Sbjct: 121  FTFTIGTRGNCEPIVCAMILWIILCLMQGNLLQAAFWYGLVVHFRVYPIIYALPIILVLD 180

Query: 1055 PCHFQYGKKPVLRKWTSSDMNSVQSSTTKIVSEKVHILVVIPRLFTKERILFGLLSGTVF 876
               F+ G+KP L  W +    +  S+  +  +   ++L  +  LF++ERI+F L+SG VF
Sbjct: 181  TQVFRSGQKPALLYWNTGQAKTPASNMER-KTFLFNLLTTLKSLFSRERIMFALISGGVF 239

Query: 875  FFLTGIFFYLYELEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYEEKFSLIEKLVSFLPQ 696
                 + FY Y  EFLHEALLYHLTRTDPRHNFSIYFYHIYLHYE +FS +EKL+SFLPQ
Sbjct: 240  LACNAVSFYFYGQEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYERQFSAVEKLISFLPQ 299

Query: 695  FLVQIVLIYRFQKDLPFCFFLQTVAFVAFNKVITAQYFVWFFCLLPLILPWSTMKLKW-G 519
            F VQ  L++ F +DL FC FLQTVAFV FNKVITAQYFVWF+CLLPLILPWS MKLKW G
Sbjct: 300  FTVQFALVFCFSQDLVFCIFLQTVAFVTFNKVITAQYFVWFYCLLPLILPWSHMKLKWEG 359

Query: 518  LICIFLWVGAQTHWLLWGYLLEFKGKQVFLFLWVAGLLFLFVNTFVIICLIIHQNCSSLF 339
            L+CI +W+GAQTHWLLWGY+LEFKG  VFL LW+A LLFL  NTFV++ +I     S LF
Sbjct: 360  LLCIIMWIGAQTHWLLWGYMLEFKGVNVFLPLWIASLLFLAANTFVLVRIIQRHRFSPLF 419

Query: 338  KPLEN 324
            +  E+
Sbjct: 420  RRYES 424