BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00026434
         (1229 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFV09099.1| sesquiterpene synthase 2 [Thapsia garganica]           564   e-158
gb|AFV09098.1| sesquiterpene synthase 1 [Thapsia garganica]           552   e-155
gb|ADK94034.1| alpha-copaene synthase [Eleutherococcus trifoliatus]   454   e-125
ref|XP_003634696.1| PREDICTED: (-)-germacrene D synthase [Vitis ...   414   e-113
sp|Q6Q3H3.1|TPSGD_VITVI RecName: Full=(-)-germacrene D synthase ...   414   e-113

>gb|AFV09099.1| sesquiterpene synthase 2 [Thapsia garganica]
          Length = 561

 Score =  564 bits (1454), Expect = e-158
 Identities = 282/408 (69%), Positives = 330/408 (80%)
 Frame = +2

Query: 5    MAQYVNSTFGPPNVVPENTIIRKSANYHPSIWGDKFLALDNSASVKTDAHCKEEERLQLL 184
            MA YVNST GPP+     +++R SA +HPSIWGD F+   NSA  KTD   KEEE LQLL
Sbjct: 1    MAVYVNSTTGPPS-----SVVRNSAGFHPSIWGDTFIPSGNSAVQKTDVDRKEEENLQLL 55

Query: 185  KNDVKKMLCAARGTPQQDDQLMISLIDDIQRLGLSYHFETEIDTILQHIQDSFCEHDYGS 364
            K +VKKML A   T QQD   +I LIDDIQRLGLSYHFE EIDT+LQH++DS+ E+ YG+
Sbjct: 56   KQEVKKMLTAG-DTCQQD---LICLIDDIQRLGLSYHFEAEIDTLLQHVKDSYLEY-YGT 110

Query: 365  KHGNGDNLHDVALCFRLLRQQGHNVSSDVFYKFKDSNGKFRENLVKDVRGMLSLFEATHL 544
            K  N DNLHDVAL FRLLRQ+GHN+SSDVF KF+DS+GKF E LVKDVRGMLSLFEA HL
Sbjct: 111  K--NHDNLHDVALSFRLLRQEGHNISSDVFSKFQDSDGKFNEKLVKDVRGMLSLFEAAHL 168

Query: 545  RVHGEDILEDALEFTTSHLNQYLKSINQMNPLASLVDRALKIPLRRGLNRLVARHYISIY 724
             VHGE+ILEDALEFTTSHLN YL S N   PLA LV RALK PLR+  NR+VARHYISIY
Sbjct: 169  SVHGENILEDALEFTTSHLNSYLNS-NPNAPLADLVRRALKYPLRKSFNRMVARHYISIY 227

Query: 725  HQFHWHNQVLLELAKCDFNLVQKLHQMELCHVTRWWKNLDVANNFPFARDRLVECYFWAT 904
            H+++WH QVLL+LAKCDFNLVQK+HQ EL ++TRWWK+LD  N  PFARDR+VECYFW T
Sbjct: 228  HKYYWHKQVLLDLAKCDFNLVQKVHQKELGYITRWWKDLDFTNKLPFARDRVVECYFWIT 287

Query: 905  GVYSEPRYAKGRIFLTKIVSMTSIVDDIYDVHGTVEELQQFTDAIEKWDISAIDQLPEYM 1084
            GVY EPRYA  R FLTK++S+TSI+DDIYDV+GT EEL Q TDAI+KWDI+ +DQLPEYM
Sbjct: 288  GVYFEPRYAAPRKFLTKVISLTSIIDDIYDVYGTPEELVQLTDAIDKWDINILDQLPEYM 347

Query: 1085 THLYQTFMDVFSEVEEDMAKAGIPSHGIGYAKDAYKRLTREYLQEAKW 1228
             H Y+  +DVF+E EE+MAK G+P++G+ YAK+A+KRLT  YL EAKW
Sbjct: 348  RHAYKPLLDVFAEGEEEMAKEGLPTYGVDYAKEAFKRLTVTYLHEAKW 395


>gb|AFV09098.1| sesquiterpene synthase 1 [Thapsia garganica]
          Length = 561

 Score =  552 bits (1423), Expect = e-155
 Identities = 275/408 (67%), Positives = 324/408 (79%)
 Frame = +2

Query: 5    MAQYVNSTFGPPNVVPENTIIRKSANYHPSIWGDKFLALDNSASVKTDAHCKEEERLQLL 184
            MA YVNST GPP  V  N     SA +HPSIWGDKF+   NSA  KTD   KEEE+LQLL
Sbjct: 1    MAVYVNSTTGPPPSVGRN-----SAGFHPSIWGDKFITSSNSAVQKTDVDRKEEEKLQLL 55

Query: 185  KNDVKKMLCAARGTPQQDDQLMISLIDDIQRLGLSYHFETEIDTILQHIQDSFCEHDYGS 364
            K +VKKML A   T QQD   +I LIDDIQRLGLSYHFE EIDT+LQH++ S+ EH YG 
Sbjct: 56   KQEVKKMLTAG-DTSQQD---LICLIDDIQRLGLSYHFEAEIDTLLQHVKHSYVEH-YGK 110

Query: 365  KHGNGDNLHDVALCFRLLRQQGHNVSSDVFYKFKDSNGKFRENLVKDVRGMLSLFEATHL 544
            K  N DNLHDVAL FRLLRQ+GHN+SSDVF KF+DS+GKF+E LV+DVRGMLSLFEA HL
Sbjct: 111  K--NHDNLHDVALSFRLLRQEGHNISSDVFSKFQDSDGKFKEELVEDVRGMLSLFEAAHL 168

Query: 545  RVHGEDILEDALEFTTSHLNQYLKSINQMNPLASLVDRALKIPLRRGLNRLVARHYISIY 724
            RVHGE+ILEDALEFTT+HLN YL S N  + LA LV RALK PLR+  NR+VARHYIS+Y
Sbjct: 169  RVHGENILEDALEFTTTHLNSYLNS-NPSSSLADLVRRALKYPLRKSFNRMVARHYISVY 227

Query: 725  HQFHWHNQVLLELAKCDFNLVQKLHQMELCHVTRWWKNLDVANNFPFARDRLVECYFWAT 904
            H+F WH QVLL++AKCDFNLVQK+HQ EL ++TRWWK+LD  N  PFARDR+VECYFW T
Sbjct: 228  HKFDWHKQVLLDMAKCDFNLVQKVHQNELGYITRWWKDLDFTNKLPFARDRVVECYFWIT 287

Query: 905  GVYSEPRYAKGRIFLTKIVSMTSIVDDIYDVHGTVEELQQFTDAIEKWDISAIDQLPEYM 1084
            GVY EPRYA  R FLT+++S+TSI+DDIYDV+GT +EL Q  DAI+KWDIS +DQLPEY+
Sbjct: 288  GVYFEPRYAAPRKFLTEVMSLTSIIDDIYDVYGTPDELVQLADAIDKWDISILDQLPEYV 347

Query: 1085 THLYQTFMDVFSEVEEDMAKAGIPSHGIGYAKDAYKRLTREYLQEAKW 1228
             H Y+  +D F+E EE+MA  G+P++G+ YAK+A+KRL   YLQE KW
Sbjct: 348  RHAYKPLLDTFAEAEEEMANEGLPTYGVDYAKEAFKRLAVAYLQETKW 395


>gb|ADK94034.1| alpha-copaene synthase [Eleutherococcus trifoliatus]
          Length = 556

 Score =  454 bits (1169), Expect = e-125
 Identities = 242/410 (59%), Positives = 301/410 (73%), Gaps = 2/410 (0%)
 Frame = +2

Query: 5    MAQYVNSTFGP-PNVVPENTIIRKSANYHPSIWGDKFLALDNSASVKTDAHCKEEERLQL 181
            MA Y+ ++ GP   +VPE  I R+SANYHP+IWGD+FL  ++    KTDA+ KE  R   
Sbjct: 1    MATYLQASSGPCSTIVPE--ITRRSANYHPNIWGDQFLKYNSFDLSKTDANTKEHFRQ-- 56

Query: 182  LKNDVKKMLCAARGTPQQDDQLMISLIDDIQRLGLSYHFETEIDTILQHIQDSFCEHDYG 361
            LK +VKKML  A G  QQ     ++LIDDIQRLG++Y FE EID  LQ +   F      
Sbjct: 57   LKEEVKKMLVDA-GPNQQ-----LNLIDDIQRLGVAYQFEAEIDAALQRMNVIF------ 104

Query: 362  SKHGNGDNLHDVALCFRLLRQQGHNVSSDVFYKFKDSNGKFRENLVKDVRGMLSLFEATH 541
               GN D+LH ++L FRLLRQ G+NVSSDVF KF D+NGKF+E L+ D+RG+LSL+EATH
Sbjct: 105  --QGNDDDLHTISLRFRLLRQHGYNVSSDVFRKFMDNNGKFKECLISDLRGVLSLYEATH 162

Query: 542  LRVHGEDILEDALEFTTSHLNQYLKSINQMNPLASLVDRALKIPLRRGLNRLVARHYISI 721
             RVHGEDILEDALEFTTSHL + LKS +  NPLA+ V RALK P+ +GLNRL A+HYISI
Sbjct: 163  FRVHGEDILEDALEFTTSHLER-LKS-HLKNPLAAQVIRALKCPIHKGLNRLEAKHYISI 220

Query: 722  YHQFH-WHNQVLLELAKCDFNLVQKLHQMELCHVTRWWKNLDVANNFPFARDRLVECYFW 898
            Y Q    HN+VLL  AK DFNL+QK+HQ EL H+TRWWK L+ A   PFARDR+VECYFW
Sbjct: 221  YQQEDDSHNKVLLNFAKLDFNLLQKMHQGELSHITRWWKELNFAKKLPFARDRVVECYFW 280

Query: 899  ATGVYSEPRYAKGRIFLTKIVSMTSIVDDIYDVHGTVEELQQFTDAIEKWDISAIDQLPE 1078
              GVY EP+Y   R FLTKI++M S+ DDIYDV+GT+EEL   TDAIE+WD+ A+DQ+PE
Sbjct: 281  ILGVYFEPQYLIARRFLTKIIAMASVADDIYDVYGTLEELVILTDAIERWDMGALDQIPE 340

Query: 1079 YMTHLYQTFMDVFSEVEEDMAKAGIPSHGIGYAKDAYKRLTREYLQEAKW 1228
             M   ++  +DV++E+EE+MAK G PS+ + YAK+AYK L R+YL EAKW
Sbjct: 341  CMRVYHRALLDVYTEMEEEMAKTGRPSYRVHYAKEAYKELVRQYLAEAKW 390


>ref|XP_003634696.1| PREDICTED: (-)-germacrene D synthase [Vitis vinifera]
            gi|302143188|emb|CBI20483.3| unnamed protein product
            [Vitis vinifera]
          Length = 557

 Score =  414 bits (1065), Expect = e-113
 Identities = 218/396 (55%), Positives = 281/396 (70%)
 Frame = +2

Query: 41   NVVPENTIIRKSANYHPSIWGDKFLALDNSASVKTDAHCKEEERLQLLKNDVKKMLCAAR 220
            N+ PE  + R+ AN+HPSIWGD FL+   S    TD H K+   +Q LK +V+KML AA 
Sbjct: 15   NLSPE--VGRRCANFHPSIWGDHFLSYA-SEFTNTDDHLKQH--VQQLKEEVRKMLMAA- 68

Query: 221  GTPQQDDQLMISLIDDIQRLGLSYHFETEIDTILQHIQDSFCEHDYGSKHGNGDNLHDVA 400
                 D    + LID IQRLG++YHFE+EID +L+H+ D       GS     ++++  +
Sbjct: 69   ---DDDSAQKLLLIDAIQRLGVAYHFESEIDEVLKHMFD-------GSVVSAEEDVYTAS 118

Query: 401  LCFRLLRQQGHNVSSDVFYKFKDSNGKFRENLVKDVRGMLSLFEATHLRVHGEDILEDAL 580
            L FRLLRQQG++VS D+F  FKD+ G F+E+L  DVRGMLSL+EATH RVHGEDIL++AL
Sbjct: 119  LRFRLLRQQGYHVSCDLFNNFKDNEGNFKESLSSDVRGMLSLYEATHFRVHGEDILDEAL 178

Query: 581  EFTTSHLNQYLKSINQMNPLASLVDRALKIPLRRGLNRLVARHYISIYHQFHWHNQVLLE 760
             FTT+HL    K  +  NPLA  V  ALK P+R+GL RL ARHY S+Y     HN+ LL+
Sbjct: 179  AFTTTHLQSATK--HSSNPLAEQVVHALKQPIRKGLPRLEARHYFSVYQADDSHNKALLK 236

Query: 761  LAKCDFNLVQKLHQMELCHVTRWWKNLDVANNFPFARDRLVECYFWATGVYSEPRYAKGR 940
            LAK DFNL+QKLHQ EL  ++ WWK+LD A+  PFARDR+VECYFW  GVY EP++   R
Sbjct: 237  LAKLDFNLLQKLHQKELSDISAWWKDLDFAHKLPFARDRVVECYFWILGVYFEPQFFFAR 296

Query: 941  IFLTKIVSMTSIVDDIYDVHGTVEELQQFTDAIEKWDISAIDQLPEYMTHLYQTFMDVFS 1120
              LTK+++MTSI+DDIYDV+GT+EEL+ FT+A+E+WDISAIDQLPEYM   YQ  + V+S
Sbjct: 297  RILTKVIAMTSIIDDIYDVYGTLEELELFTEAVERWDISAIDQLPEYMRVCYQALLYVYS 356

Query: 1121 EVEEDMAKAGIPSHGIGYAKDAYKRLTREYLQEAKW 1228
            E+EE+MAK G  S+ + YAK+A K   R Y +EAKW
Sbjct: 357  EIEEEMAKEG-RSYRLYYAKEAMKNQVRAYYEEAKW 391


>sp|Q6Q3H3.1|TPSGD_VITVI RecName: Full=(-)-germacrene D synthase gi|45478053|gb|AAS66357.1|
            (-)-germacrene D synthase [Vitis vinifera]
          Length = 557

 Score =  414 bits (1065), Expect = e-113
 Identities = 218/396 (55%), Positives = 281/396 (70%)
 Frame = +2

Query: 41   NVVPENTIIRKSANYHPSIWGDKFLALDNSASVKTDAHCKEEERLQLLKNDVKKMLCAAR 220
            N+ PE  + R+ AN+HPSIWGD FL+   S    TD H K+   +Q LK +V+KML AA 
Sbjct: 15   NLSPE--VGRRCANFHPSIWGDHFLSYA-SEFTNTDDHLKQH--VQQLKEEVRKMLMAA- 68

Query: 221  GTPQQDDQLMISLIDDIQRLGLSYHFETEIDTILQHIQDSFCEHDYGSKHGNGDNLHDVA 400
                 D    + LID IQRLG++YHFE+EID +L+H+ D       GS     ++++  +
Sbjct: 69   ---DDDSAQKLLLIDAIQRLGVAYHFESEIDEVLKHMFD-------GSVVSAEEDVYTAS 118

Query: 401  LCFRLLRQQGHNVSSDVFYKFKDSNGKFRENLVKDVRGMLSLFEATHLRVHGEDILEDAL 580
            L FRLLRQQG++VS D+F  FKD+ G F+E+L  DVRGMLSL+EATH RVHGEDIL++AL
Sbjct: 119  LRFRLLRQQGYHVSCDLFNNFKDNEGNFKESLSSDVRGMLSLYEATHFRVHGEDILDEAL 178

Query: 581  EFTTSHLNQYLKSINQMNPLASLVDRALKIPLRRGLNRLVARHYISIYHQFHWHNQVLLE 760
             FTT+HL    K  +  NPLA  V  ALK P+R+GL RL ARHY S+Y     HN+ LL+
Sbjct: 179  AFTTTHLQSATK--HSSNPLAEQVVHALKQPIRKGLPRLEARHYFSVYQADDSHNKALLK 236

Query: 761  LAKCDFNLVQKLHQMELCHVTRWWKNLDVANNFPFARDRLVECYFWATGVYSEPRYAKGR 940
            LAK DFNL+QKLHQ EL  ++ WWK+LD A+  PFARDR+VECYFW  GVY EP++   R
Sbjct: 237  LAKLDFNLLQKLHQKELSDISAWWKDLDFAHKLPFARDRVVECYFWILGVYFEPQFFFAR 296

Query: 941  IFLTKIVSMTSIVDDIYDVHGTVEELQQFTDAIEKWDISAIDQLPEYMTHLYQTFMDVFS 1120
              LTK+++MTSI+DDIYDV+GT+EEL+ FT+A+E+WDISAIDQLPEYM   YQ  + V+S
Sbjct: 297  RILTKVIAMTSIIDDIYDVYGTLEELELFTEAVERWDISAIDQLPEYMRVCYQALLYVYS 356

Query: 1121 EVEEDMAKAGIPSHGIGYAKDAYKRLTREYLQEAKW 1228
            E+EE+MAK G  S+ + YAK+A K   R Y +EAKW
Sbjct: 357  EIEEEMAKEG-RSYRLYYAKEAMKNQVRAYYEEAKW 391