BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00026264
         (962 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AED99877.1| cytochrome P450 [Panax notoginseng]                    506   e-141
ref|XP_002282788.1| PREDICTED: abscisic acid 8'-hydroxylase 4 [V...   474   e-131
emb|CAN82146.1| hypothetical protein VITISV_043497 [Vitis vinifera]   474   e-131
gb|AEX15514.1| ABA 8'-hydroxylase [Citrus sinensis]                   461   e-127
ref|XP_002327141.1| cytochrome P450 [Populus trichocarpa] gi|222...   455   e-126

>gb|AED99877.1| cytochrome P450 [Panax notoginseng]
          Length = 471

 Score =  506 bits (1304), Expect = e-141
 Identities = 251/318 (78%), Positives = 277/318 (87%)
 Frame = -3

Query: 957 MESCAVLLYISITFLLALMAYILFATRNRDGHNKTKLPPGSMGWPYIGETVQLYCQDPSV 778
           ME    L+Y+ I+FLL L+ YIL     R+   K KLPPGSMGWPYIGET+QLY QDPSV
Sbjct: 1   MEKGGFLVYV-ISFLLILLIYILLKRNERESKRKAKLPPGSMGWPYIGETLQLYSQDPSV 59

Query: 777 FFANKQKRYGEIFKTHVLGCPCVMLASPEASRLVLTTHAHLFKPTYPKSKENLIGPSALF 598
           FF  KQ+RYG+IFKT++LGCPCVMLASPEASR VL T AHLFKPTYPKSKENLIGPSALF
Sbjct: 60  FFTTKQRRYGDIFKTYLLGCPCVMLASPEASRFVLMTQAHLFKPTYPKSKENLIGPSALF 119

Query: 597 FHQGDYHARLRKLVQSSLSPDAIRNSVSGIEAIAVSALDSWASTHVINTFTEMKKASFEV 418
           FHQG+YH+RLRKLVQ S+SPDAIRN V+GIEAIAVSALDSWA  HVINTF EMKK SFEV
Sbjct: 120 FHQGEYHSRLRKLVQGSMSPDAIRNLVTGIEAIAVSALDSWAGEHVINTFNEMKKFSFEV 179

Query: 417 GILTIFGHLESHYKHELKKNYSIVDGGYNSFPSNLPGTPYRKALLARKRLSEILSEIIRE 238
           GILTIFGHLE+ +K ELK+NYSIVD GYNSFPSNLPGTPY+KALLARKRLS+I+ EII E
Sbjct: 180 GILTIFGHLEACHKEELKRNYSIVDRGYNSFPSNLPGTPYKKALLARKRLSKIVGEIICE 239

Query: 237 RKEKMSHEKDLLGCLLNYKDDNGDILNENQIADNIIGVFFAAQDTTASVMTWILKYLYDN 58
           RKEK S EKDLLGCLLN+KD+ G+ILNE+QIADNIIGV FAAQDTTAS MTWILKYLYDN
Sbjct: 240 RKEKRSLEKDLLGCLLNFKDEKGEILNEDQIADNIIGVLFAAQDTTASAMTWILKYLYDN 299

Query: 57  PKLLEVIKAEHKAIHMSN 4
           PKL+E  KAE KAI+MSN
Sbjct: 300 PKLVETAKAEQKAIYMSN 317


>ref|XP_002282788.1| PREDICTED: abscisic acid 8'-hydroxylase 4 [Vitis vinifera]
          Length = 470

 Score =  474 bits (1219), Expect = e-131
 Identities = 239/318 (75%), Positives = 267/318 (83%)
 Frame = -3

Query: 957 MESCAVLLYISITFLLALMAYILFATRNRDGHNKTKLPPGSMGWPYIGETVQLYCQDPSV 778
           ME    L Y+ I FLLAL+ YIL     R+  ++ KLPPGSMGWPYIGET+QLY QDPSV
Sbjct: 1   MEKPGFLAYVVI-FLLALLFYIL-RREKREPKHRAKLPPGSMGWPYIGETLQLYSQDPSV 58

Query: 777 FFANKQKRYGEIFKTHVLGCPCVMLASPEASRLVLTTHAHLFKPTYPKSKENLIGPSALF 598
           FFA KQKRYGEIFKTH+LGCPCVMLASPEA+R VL T AHLFKPTYPKSKE LIGPSALF
Sbjct: 59  FFAAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALF 118

Query: 597 FHQGDYHARLRKLVQSSLSPDAIRNSVSGIEAIAVSALDSWASTHVINTFTEMKKASFEV 418
           FHQGDYH RLRKLVQ SLSP+AIRN V+ IEA+AVSALDSWA  HV NTF E+KK SFEV
Sbjct: 119 FHQGDYHNRLRKLVQGSLSPEAIRNLVADIEALAVSALDSWAGGHVFNTFHEIKKFSFEV 178

Query: 417 GILTIFGHLESHYKHELKKNYSIVDGGYNSFPSNLPGTPYRKALLARKRLSEILSEIIRE 238
           GIL IFG LE+HY+ ELKKNY IVD GYNSFP+N+PGTPY+KAL ARKRLS+ILSE+I +
Sbjct: 179 GILAIFGRLEAHYREELKKNYCIVDRGYNSFPTNIPGTPYKKALWARKRLSKILSELIGQ 238

Query: 237 RKEKMSHEKDLLGCLLNYKDDNGDILNENQIADNIIGVFFAAQDTTASVMTWILKYLYDN 58
           RKEK   +KDLLG LLN KD+ G+ L  +QIADNIIGV FAAQDTTASVMTWILKYL+D+
Sbjct: 239 RKEKRLADKDLLGSLLNSKDEKGETLTNDQIADNIIGVLFAAQDTTASVMTWILKYLHDD 298

Query: 57  PKLLEVIKAEHKAIHMSN 4
           PKLLE +KAE KAI+ SN
Sbjct: 299 PKLLEAVKAEQKAINKSN 316


>emb|CAN82146.1| hypothetical protein VITISV_043497 [Vitis vinifera]
          Length = 608

 Score =  474 bits (1219), Expect = e-131
 Identities = 239/318 (75%), Positives = 267/318 (83%)
 Frame = -3

Query: 957 MESCAVLLYISITFLLALMAYILFATRNRDGHNKTKLPPGSMGWPYIGETVQLYCQDPSV 778
           ME    L Y+ I FLLAL+ YIL     R+  ++ KLPPGSMGWPYIGET+QLY QDPSV
Sbjct: 1   MEKPGFLAYVVI-FLLALLFYIL-RREKREPKHRAKLPPGSMGWPYIGETLQLYSQDPSV 58

Query: 777 FFANKQKRYGEIFKTHVLGCPCVMLASPEASRLVLTTHAHLFKPTYPKSKENLIGPSALF 598
           FFA KQKRYGEIFKTH+LGCPCVMLASPEA+R VL T AHLFKPTYPKSKE LIGPSALF
Sbjct: 59  FFAAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALF 118

Query: 597 FHQGDYHARLRKLVQSSLSPDAIRNSVSGIEAIAVSALDSWASTHVINTFTEMKKASFEV 418
           FHQGDYH RLRKLVQ SLSP+AIRN V+ IEA+AVSALDSWA  HV NTF E+KK SFEV
Sbjct: 119 FHQGDYHNRLRKLVQGSLSPEAIRNLVADIEALAVSALDSWAGGHVFNTFHEIKKFSFEV 178

Query: 417 GILTIFGHLESHYKHELKKNYSIVDGGYNSFPSNLPGTPYRKALLARKRLSEILSEIIRE 238
           GIL IFG LE+HY+ ELKKNY IVD GYNSFP+N+PGTPY+KAL ARKRLS+ILSE+I +
Sbjct: 179 GILAIFGRLEAHYREELKKNYCIVDRGYNSFPTNIPGTPYKKALWARKRLSKILSELIGQ 238

Query: 237 RKEKMSHEKDLLGCLLNYKDDNGDILNENQIADNIIGVFFAAQDTTASVMTWILKYLYDN 58
           RKEK   +KDLLG LLN KD+ G+ L  +QIADNIIGV FAAQDTTASVMTWILKYL+D+
Sbjct: 239 RKEKRLADKDLLGSLLNSKDEKGETLTNDQIADNIIGVLFAAQDTTASVMTWILKYLHDD 298

Query: 57  PKLLEVIKAEHKAIHMSN 4
           PKLLE +KAE KAI+ SN
Sbjct: 299 PKLLEAVKAEQKAINKSN 316


>gb|AEX15514.1| ABA 8'-hydroxylase [Citrus sinensis]
          Length = 470

 Score =  461 bits (1185), Expect = e-127
 Identities = 228/313 (72%), Positives = 259/313 (82%)
 Frame = -3

Query: 942 VLLYISITFLLALMAYILFATRNRDGHNKTKLPPGSMGWPYIGETVQLYCQDPSVFFANK 763
           +LL   I  L AL   +LFA    +   K KLPPGSMGWP IGET+QLY QDP+VFF  K
Sbjct: 4   ILLCFFIFLLAALPLCMLFARDKTEPRKKAKLPPGSMGWPLIGETLQLYSQDPNVFFTAK 63

Query: 762 QKRYGEIFKTHVLGCPCVMLASPEASRLVLTTHAHLFKPTYPKSKENLIGPSALFFHQGD 583
           QKRYGEIFKTH+LGCPCVMLAS EA++ VL T AHLFKPTYPKSKE LIGPSA+FFHQGD
Sbjct: 64  QKRYGEIFKTHILGCPCVMLASSEAAKFVLVTQAHLFKPTYPKSKERLIGPSAIFFHQGD 123

Query: 582 YHARLRKLVQSSLSPDAIRNSVSGIEAIAVSALDSWASTHVINTFTEMKKASFEVGILTI 403
           YH RLRK+VQ SLS +AIR  V+ IEA AV+ALDSW   H+INTF EMKK SFEVG LTI
Sbjct: 124 YHLRLRKIVQGSLSLEAIRELVTDIEAAAVAALDSWHGGHIINTFQEMKKLSFEVGTLTI 183

Query: 402 FGHLESHYKHELKKNYSIVDGGYNSFPSNLPGTPYRKALLARKRLSEILSEIIRERKEKM 223
           FGHL+ +YK ELKKNY IVD GYNSF +N+PGTPY+KAL ARKRL+EILS+IIRERKEK 
Sbjct: 184 FGHLKDNYKGELKKNYLIVDKGYNSFSTNIPGTPYKKALQARKRLNEILSDIIRERKEKR 243

Query: 222 SHEKDLLGCLLNYKDDNGDILNENQIADNIIGVFFAAQDTTASVMTWILKYLYDNPKLLE 43
            HEKDLLGCLLN KD+NG++L++++IADNIIGV FAAQDTTASVMTWI+KYL+DNPKLLE
Sbjct: 244 LHEKDLLGCLLNSKDENGEVLSDDKIADNIIGVLFAAQDTTASVMTWIVKYLHDNPKLLE 303

Query: 42  VIKAEHKAIHMSN 4
            +KAE KAI   N
Sbjct: 304 GVKAEQKAIRKLN 316


>ref|XP_002327141.1| cytochrome P450 [Populus trichocarpa] gi|222835456|gb|EEE73891.1|
           cytochrome P450 [Populus trichocarpa]
          Length = 469

 Score =  455 bits (1171), Expect = e-126
 Identities = 222/314 (70%), Positives = 263/314 (83%)
 Frame = -3

Query: 942 VLLYISITFLLALMAYILFATRNRDGHNKTKLPPGSMGWPYIGETVQLYCQDPSVFFANK 763
           +L Y+ I FL++++AYI+    N++ +   KLPPGS+GWPYIGET+QLY QDP+VFFA+K
Sbjct: 4   ILAYVLI-FLVSVVAYIIKKRNNKEPYTGIKLPPGSLGWPYIGETLQLYSQDPNVFFASK 62

Query: 762 QKRYGEIFKTHVLGCPCVMLASPEASRLVLTTHAHLFKPTYPKSKENLIGPSALFFHQGD 583
           QKRYGEIFKTH+LGCP VMLASPEA+R VL T AHLFKPTYPKSKE+LIGPSALFFHQGD
Sbjct: 63  QKRYGEIFKTHILGCPSVMLASPEAARFVLVTQAHLFKPTYPKSKEHLIGPSALFFHQGD 122

Query: 582 YHARLRKLVQSSLSPDAIRNSVSGIEAIAVSALDSWASTHVINTFTEMKKASFEVGILTI 403
           YH RLRKLVQ SLS DAIRN V+ I A A S LDSW   HV+NTF EMKK SFEVGIL I
Sbjct: 123 YHVRLRKLVQGSLSLDAIRNLVADISATAASTLDSWDGGHVLNTFQEMKKFSFEVGILAI 182

Query: 402 FGHLESHYKHELKKNYSIVDGGYNSFPSNLPGTPYRKALLARKRLSEILSEIIRERKEKM 223
           FG+LE+HY+ ELK+NY IVD GYNSFP++LPGTP++KA+LARKRLS+IL +II ERKEK 
Sbjct: 183 FGNLEAHYREELKRNYRIVDKGYNSFPTSLPGTPFKKAVLARKRLSKILGDIICERKEKR 242

Query: 222 SHEKDLLGCLLNYKDDNGDILNENQIADNIIGVFFAAQDTTASVMTWILKYLYDNPKLLE 43
             EKDLLGCLLN KD+ G++L ++QIADNIIGV FAAQDTTA+ MTWI+KYL+DN K+LE
Sbjct: 243 LLEKDLLGCLLNSKDEKGEVLTDDQIADNIIGVLFAAQDTTATAMTWIVKYLHDNQKILE 302

Query: 42  VIKAEHKAIHMSNN 1
            +KAE  AI   N+
Sbjct: 303 AVKAEQNAIRKLND 316