BLASTX nr result
ID: Bupleurum21_contig00026130
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00026130
(867 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002272635.2| PREDICTED: uncharacterized protein LOC100254... 325 6e-87
emb|CBI38830.3| unnamed protein product [Vitis vinifera] 325 6e-87
ref|XP_003623809.1| Xylem serine proteinase [Medicago truncatula... 308 1e-81
ref|XP_002875042.1| hypothetical protein ARALYDRAFT_490544 [Arab... 306 4e-81
gb|AAC19302.1| contains similarity to the subtilase family of se... 305 7e-81
>ref|XP_002272635.2| PREDICTED: uncharacterized protein LOC100254106 [Vitis vinifera]
Length = 1522
Score = 325 bits (834), Expect = 6e-87
Identities = 171/316 (54%), Positives = 205/316 (64%), Gaps = 59/316 (18%)
Frame = -2
Query: 773 GITPKAESFNDHGLGPPPVKWKGSCQKSANFSGCNNKLIGAQYFKLDNRTPDPQDILSPV 594
GITP++ESF D+G GPPP KWKGSC + ANFSGCNNKLIGA+YFKLD + PDP DILSPV
Sbjct: 138 GITPQSESFADNGFGPPPAKWKGSCGRFANFSGCNNKLIGAKYFKLDGK-PDPDDILSPV 196
Query: 593 DADGHGTHTSSTVAGALVQNATLFGLAKGTARGAVPSARIAMYKVCWRSFGCSDMDILAG 414
D +GHGTHT+STVAG +V+NA LFGLAKGTARGAVPSAR+AMYKVCW S GCSDMD+LAG
Sbjct: 197 DVEGHGTHTASTVAGNIVKNANLFGLAKGTARGAVPSARVAMYKVCWVSTGCSDMDLLAG 256
Query: 413 FEAAIQXXXXXXXXXXXXXXXSFDTDSIAVGSFHAMRKGIITVASAGNNGPDLASVTNHA 234
FEAAI ++ D IA+G+FHAM+KGI+T+ASAGN+GPD +++ NHA
Sbjct: 257 FEAAIADGVDVISISIGGFTFNYAEDIIAIGAFHAMKKGILTIASAGNDGPDESTIVNHA 316
Query: 233 PWIFTVAASGISRQFKSKVV-------LGNG----------------------------- 162
PWI TV ASGI R F+SKVV LG+G
Sbjct: 317 PWILTVGASGIDRSFRSKVVLGNGKTFLGSGLSAFDPKQKNYPLVSGADIPKTKADKENS 376
Query: 161 -----------------------QTGIDSVVKDLGGIGTIIQSDMFLDAAMIFMAPSTTV 51
+ G++SVVK LGGIG I++S +FLD IFMAP T +
Sbjct: 377 RFCIEDSLDPTKVKGKLVYCELEEWGVESVVKGLGGIGAIVESTVFLDTPQIFMAPGTMI 436
Query: 50 NYTTGEVINKYIKSTR 3
N T G+ I+ YI STR
Sbjct: 437 NDTVGQAIDGYIHSTR 452
Score = 160 bits (404), Expect = 5e-37
Identities = 89/215 (41%), Positives = 125/215 (58%), Gaps = 8/215 (3%)
Frame = -2
Query: 776 AGITPKAESFNDHGLGPP-PVKWKGSCQKSANFSG--CNNKLIGAQ-YFK----LDNRTP 621
+GI P+ +SF D G+ P P +WKG C++ F+ CN KLIGA+ Y+K +
Sbjct: 904 SGIWPEHDSFKDRGMTRPVPSRWKGVCEQGTKFTAKNCNKKLIGARAYYKGYEATAGKID 963
Query: 620 DPQDILSPVDADGHGTHTSSTVAGALVQNATLFGLAKGTARGAVPSARIAMYKVCWRSFG 441
+ D S D+ GHGTHT+ST AG ++ A+ FG+AKG A G +ARIA YK C+ G
Sbjct: 964 ETVDFRSARDSQGHGTHTASTAAGHMIDGASSFGMAKGVAAGMSCTARIAAYKACYAG-G 1022
Query: 440 CSDMDILAGFEAAIQXXXXXXXXXXXXXXXSFDTDSIAVGSFHAMRKGIITVASAGNNGP 261
C+ DILA + A+ + TD +A+ S A++ GI A+AGN+GP
Sbjct: 1023 CATSDILAAIDQAVSDGVDVLSLSIGGSSQPYYTDVLAIASLGAVQHGIFVAAAAGNSGP 1082
Query: 260 DLASVTNHAPWIFTVAASGISRQFKSKVVLGNGQT 156
++V N APW+ TVAAS + R F + V LGNG+T
Sbjct: 1083 SSSTVINTAPWMMTVAASTMDRSFTAIVNLGNGET 1117
>emb|CBI38830.3| unnamed protein product [Vitis vinifera]
Length = 1470
Score = 325 bits (834), Expect = 6e-87
Identities = 171/316 (54%), Positives = 205/316 (64%), Gaps = 59/316 (18%)
Frame = -2
Query: 773 GITPKAESFNDHGLGPPPVKWKGSCQKSANFSGCNNKLIGAQYFKLDNRTPDPQDILSPV 594
GITP++ESF D+G GPPP KWKGSC + ANFSGCNNKLIGA+YFKLD + PDP DILSPV
Sbjct: 216 GITPQSESFADNGFGPPPAKWKGSCGRFANFSGCNNKLIGAKYFKLDGK-PDPDDILSPV 274
Query: 593 DADGHGTHTSSTVAGALVQNATLFGLAKGTARGAVPSARIAMYKVCWRSFGCSDMDILAG 414
D +GHGTHT+STVAG +V+NA LFGLAKGTARGAVPSAR+AMYKVCW S GCSDMD+LAG
Sbjct: 275 DVEGHGTHTASTVAGNIVKNANLFGLAKGTARGAVPSARVAMYKVCWVSTGCSDMDLLAG 334
Query: 413 FEAAIQXXXXXXXXXXXXXXXSFDTDSIAVGSFHAMRKGIITVASAGNNGPDLASVTNHA 234
FEAAI ++ D IA+G+FHAM+KGI+T+ASAGN+GPD +++ NHA
Sbjct: 335 FEAAIADGVDVISISIGGFTFNYAEDIIAIGAFHAMKKGILTIASAGNDGPDESTIVNHA 394
Query: 233 PWIFTVAASGISRQFKSKVV-------LGNG----------------------------- 162
PWI TV ASGI R F+SKVV LG+G
Sbjct: 395 PWILTVGASGIDRSFRSKVVLGNGKTFLGSGLSAFDPKQKNYPLVSGADIPKTKADKENS 454
Query: 161 -----------------------QTGIDSVVKDLGGIGTIIQSDMFLDAAMIFMAPSTTV 51
+ G++SVVK LGGIG I++S +FLD IFMAP T +
Sbjct: 455 RFCIEDSLDPTKVKGKLVYCELEEWGVESVVKGLGGIGAIVESTVFLDTPQIFMAPGTMI 514
Query: 50 NYTTGEVINKYIKSTR 3
N T G+ I+ YI STR
Sbjct: 515 NDTVGQAIDGYIHSTR 530
Score = 160 bits (404), Expect = 5e-37
Identities = 89/215 (41%), Positives = 125/215 (58%), Gaps = 8/215 (3%)
Frame = -2
Query: 776 AGITPKAESFNDHGLGPP-PVKWKGSCQKSANFSG--CNNKLIGAQ-YFK----LDNRTP 621
+GI P+ +SF D G+ P P +WKG C++ F+ CN KLIGA+ Y+K +
Sbjct: 1004 SGIWPEHDSFKDRGMTRPVPSRWKGVCEQGTKFTAKNCNKKLIGARAYYKGYEATAGKID 1063
Query: 620 DPQDILSPVDADGHGTHTSSTVAGALVQNATLFGLAKGTARGAVPSARIAMYKVCWRSFG 441
+ D S D+ GHGTHT+ST AG ++ A+ FG+AKG A G +ARIA YK C+ G
Sbjct: 1064 ETVDFRSARDSQGHGTHTASTAAGHMIDGASSFGMAKGVAAGMSCTARIAAYKACYAG-G 1122
Query: 440 CSDMDILAGFEAAIQXXXXXXXXXXXXXXXSFDTDSIAVGSFHAMRKGIITVASAGNNGP 261
C+ DILA + A+ + TD +A+ S A++ GI A+AGN+GP
Sbjct: 1123 CATSDILAAIDQAVSDGVDVLSLSIGGSSQPYYTDVLAIASLGAVQHGIFVAAAAGNSGP 1182
Query: 260 DLASVTNHAPWIFTVAASGISRQFKSKVVLGNGQT 156
++V N APW+ TVAAS + R F + V LGNG+T
Sbjct: 1183 SSSTVINTAPWMMTVAASTMDRSFTAIVNLGNGET 1217
>ref|XP_003623809.1| Xylem serine proteinase [Medicago truncatula]
gi|355498824|gb|AES80027.1| Xylem serine proteinase
[Medicago truncatula]
Length = 900
Score = 308 bits (789), Expect = 1e-81
Identities = 164/315 (52%), Positives = 197/315 (62%), Gaps = 58/315 (18%)
Frame = -2
Query: 773 GITPKAESFNDHGLGPPPVKWKGSCQKSANFSGCNNKLIGAQYFKLDNRTPDPQDILSPV 594
GITP+++SF D GLGPPP KWKG+C+ ANFSGCNNK+IGA+YFK D PDP DILSP+
Sbjct: 302 GITPESKSFKDDGLGPPPAKWKGTCKHYANFSGCNNKIIGAKYFKADGN-PDPADILSPI 360
Query: 593 DADGHGTHTSSTVAGALVQNATLFGLAKGTARGAVPSARIAMYKVCWRSFGCSDMDILAG 414
D DGHGTHT+ST AG LVQNA LFGLA GT+RGAVPSAR+A+YKVCW S GC+DMDILA
Sbjct: 361 DVDGHGTHTASTAAGDLVQNANLFGLANGTSRGAVPSARLAIYKVCWSSTGCADMDILAA 420
Query: 413 FEAAIQXXXXXXXXXXXXXXXSFDTDSIAVGSFHAMRKGIITVASAGNNGPDLASVTNHA 234
FEAAI + DSI++G+FHAMRKGIITVASAGN+GP + +VTN A
Sbjct: 421 FEAAIHDGVDVISISIGGGSPDYVHDSISIGAFHAMRKGIITVASAGNDGPSMGTVTNTA 480
Query: 233 PWIFTVAASGISRQFKSKVVLGNGQ--------------------TGID----------- 147
PWI T AASGI R FKS V LG+G+ GID
Sbjct: 481 PWIVTAAASGIDRAFKSTVQLGSGKNVSGVGISCFDPKQNRYPIINGIDAAKDSKSKEDA 540
Query: 146 ---------------------------SVVKDLGGIGTIIQSDMFLDAAMIFMAPSTTVN 48
+ VK++GGIG++I+ D + D A I +AP+ VN
Sbjct: 541 KFCNSGSLQANKVKGKLVYCIGSWGTEATVKEIGGIGSVIEYDNYPDVAQISIAPAAIVN 600
Query: 47 YTTGEVINKYIKSTR 3
++ GE I YIKSTR
Sbjct: 601 HSIGETITNYIKSTR 615
>ref|XP_002875042.1| hypothetical protein ARALYDRAFT_490544 [Arabidopsis lyrata subsp.
lyrata] gi|297320879|gb|EFH51301.1| hypothetical protein
ARALYDRAFT_490544 [Arabidopsis lyrata subsp. lyrata]
Length = 752
Score = 306 bits (784), Expect = 4e-81
Identities = 165/316 (52%), Positives = 198/316 (62%), Gaps = 59/316 (18%)
Frame = -2
Query: 773 GITPKAESFNDHGLGPPPVKWKGSCQKSANFSGCNNKLIGAQYFKLDNRTPDPQDILSPV 594
GITP++ESF+DHGLGPPP KWKGSC NF+GCNNK+IGA+YFK D P + I SP+
Sbjct: 150 GITPESESFHDHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKHDGNVPTGE-IRSPI 208
Query: 593 DADGHGTHTSSTVAGALVQNATLFGLAKGTARGAVPSARIAMYKVCWRSFGCSDMDILAG 414
D DGHGTHTSSTVAG LV NA+L+G+A GTARGAVPSAR+AMYKVCW GC+DMDILAG
Sbjct: 209 DIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCWERSGCADMDILAG 268
Query: 413 FEAAIQXXXXXXXXXXXXXXXSFDTDSIAVGSFHAMRKGIITVASAGNNGPDLASVTNHA 234
FEAAI + +DSI+VGSFHAMRKGI+TVASAGN+GP +VTNH
Sbjct: 269 FEAAIHDGVDIISISIGGPIADYSSDSISVGSFHAMRKGILTVASAGNDGPSSGTVTNHE 328
Query: 233 PWIFTVAASGISRQFKSKVVLGNGQ--------------------TGIDS---------- 144
PWI TVAASGI R FKSK+ LGNG+ +G+D+
Sbjct: 329 PWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFNPKAKSYPLVSGVDAAKTTDDKYLA 388
Query: 143 -----------------VVKDLGG------------IGTIIQSDMFLDAAMIFMAPSTTV 51
+V +GG G II SD + D A IFMAP+T+V
Sbjct: 389 RYCFSDSLDRKKVKGKVMVCRMGGGGVESTVKSYGGAGAIIVSDQYQDNAQIFMAPATSV 448
Query: 50 NYTTGEVINKYIKSTR 3
N + G++I +YI STR
Sbjct: 449 NSSVGDIIYRYINSTR 464
>gb|AAC19302.1| contains similarity to the subtilase family of serine proteases
(Pfam: subtilase.hmm, score: 47.57); strong similarity to
Cucumis melo (muskmelon) cucumisin (GB:D32206)
[Arabidopsis thaliana] gi|7267110|emb|CAB80781.1|
putative cucumisin protease [Arabidopsis thaliana]
Length = 706
Score = 305 bits (782), Expect = 7e-81
Identities = 165/316 (52%), Positives = 197/316 (62%), Gaps = 59/316 (18%)
Frame = -2
Query: 773 GITPKAESFNDHGLGPPPVKWKGSCQKSANFSGCNNKLIGAQYFKLDNRTPDPQDILSPV 594
GITP +ESF DHGLGPPP KWKGSC NF+GCNNK+IGA+YFK D P ++ SP+
Sbjct: 91 GITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKHDGNVP-AGEVRSPI 149
Query: 593 DADGHGTHTSSTVAGALVQNATLFGLAKGTARGAVPSARIAMYKVCWRSFGCSDMDILAG 414
D DGHGTHTSSTVAG LV NA+L+G+A GTARGAVPSAR+AMYKVCW GC+DMDILAG
Sbjct: 150 DIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCWARSGCADMDILAG 209
Query: 413 FEAAIQXXXXXXXXXXXXXXXSFDTDSIAVGSFHAMRKGIITVASAGNNGPDLASVTNHA 234
FEAAI + +DSI+VGSFHAMRKGI+TVASAGN+GP +VTNH
Sbjct: 210 FEAAIHDGVEIISISIGGPIADYSSDSISVGSFHAMRKGILTVASAGNDGPSSGTVTNHE 269
Query: 233 PWIFTVAASGISRQFKSKVVLGNGQ--------------------TGIDS---------- 144
PWI TVAASGI R FKSK+ LGNG+ +G+D+
Sbjct: 270 PWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVSGVDAAKNTDDKYLA 329
Query: 143 -----------------VVKDLGG------------IGTIIQSDMFLDAAMIFMAPSTTV 51
+V +GG G II SD +LD A IFMAP+T+V
Sbjct: 330 RYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQYLDNAQIFMAPATSV 389
Query: 50 NYTTGEVINKYIKSTR 3
N + G++I +YI STR
Sbjct: 390 NSSVGDIIYRYINSTR 405