BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00025812
         (740 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADK94034.1| alpha-copaene synthase [Eleutherococcus trifoliatus]   263   3e-68
gb|AAX16121.1| germacrene-D synthase [Actinidia deliciosa]            256   3e-66
gb|AFV09099.1| sesquiterpene synthase 2 [Thapsia garganica]           252   5e-65
gb|AFV09098.1| sesquiterpene synthase 1 [Thapsia garganica]           244   1e-62
gb|ABK63808.1| sesquiterpene cyclase [Centella asiatica]              243   2e-62

>gb|ADK94034.1| alpha-copaene synthase [Eleutherococcus trifoliatus]
          Length = 556

 Score =  263 bits (672), Expect = 3e-68
 Identities = 134/241 (55%), Positives = 173/241 (71%)
 Frame = +3

Query: 3    DAIEKWDTSCLNMLPEYMQVCYQALLDTYSEMEKVLDKEAGRPTYRVSEAKNSFKRLAKA 182
            DAIE+WD   L+ +PE M+V ++ALLD Y+EME+ + K  GRP+YRV  AK ++K L + 
Sbjct: 325  DAIERWDMGALDQIPECMRVYHRALLDVYTEMEEEMAK-TGRPSYRVHYAKEAYKELVRQ 383

Query: 183  YLDEAKWYKEGYFPTTVEEYMKVGLVSSGYGTMATHCFVGMGDEATREVFHWVSNDPLIV 362
            YL EAKW++E Y PT +EEY+ V L+S GY  +ATH FVGMGD AT+E F WVSN+PLIV
Sbjct: 384  YLAEAKWFQEDYDPT-LEEYLPVALISGGYKMLATHSFVGMGDLATKEAFDWVSNNPLIV 442

Query: 363  RASCLIARLCDDMAGHEFEQEKGDIPSAVECYMKQHCATKEEAIAELQEIVVNAWKDINQ 542
            +AS +I RL DDM GHE E E+GD+ SAVECYMKQ+  TK+E   E Q+ + NAWKD+NQ
Sbjct: 443  KASSVICRLSDDMVGHEVEHERGDVASAVECYMKQYGVTKQEVYIEFQKQISNAWKDMNQ 502

Query: 543  EYYLSCVPAPXXXXXXIIPSSLLARIDNLTRAIHVVYDGDDGYTHSSTRTKDLITSVLID 722
            E    C+          +   LL  I N+TR I+++YD +DGYT+S+TRTKD ITSVLID
Sbjct: 503  E----CL------HPTTVTMPLLTVIFNMTRVINLLYDEEDGYTNSNTRTKDFITSVLID 552

Query: 723  P 725
            P
Sbjct: 553  P 553


>gb|AAX16121.1| germacrene-D synthase [Actinidia deliciosa]
          Length = 565

 Score =  256 bits (654), Expect = 3e-66
 Identities = 130/244 (53%), Positives = 176/244 (72%)
 Frame = +3

Query: 3    DAIEKWDTSCLNMLPEYMQVCYQALLDTYSEMEKVLDKEAGRPTYRVSEAKNSFKRLAKA 182
            DAIE+W+ S L+ LPEYM++CYQALLD YS +++ + K+ GR +Y V  AK+S K L +A
Sbjct: 334  DAIERWEISALDQLPEYMKLCYQALLDVYSMIDEEMAKQ-GR-SYCVDYAKSSMKILVRA 391

Query: 183  YLDEAKWYKEGYFPTTVEEYMKVGLVSSGYGTMATHCFVGMGDEATREVFHWVSNDPLIV 362
            Y +EAKW+ +GY PT +EEYM+V LV++GY  +AT  FVGMGD AT+E F WVSNDPLIV
Sbjct: 392  YFEEAKWFHQGYVPT-MEEYMQVALVTAGYKMLATSSFVGMGDLATKEAFDWVSNDPLIV 450

Query: 363  RASCLIARLCDDMAGHEFEQEKGDIPSAVECYMKQHCATKEEAIAELQEIVVNAWKDINQ 542
            +A+ +I RL DD+ GH+FEQ++G + SAVECY KQH  T+EEAI EL + V ++WKDIN 
Sbjct: 451  QAASVIGRLKDDIVGHKFEQKRGHVASAVECYSKQHGTTEEEAIIELDKQVTHSWKDINA 510

Query: 543  EYYLSCVPAPXXXXXXIIPSSLLARIDNLTRAIHVVYDGDDGYTHSSTRTKDLITSVLID 722
            E    C+          +P  LLAR+ NL R ++V+Y  +DGYTH  T+ ++ +TSVLID
Sbjct: 511  E----CL------CPIKVPMPLLARVLNLARVLYVIYQDEDGYTHPGTKVENFVTSVLID 560

Query: 723  PFPL 734
              P+
Sbjct: 561  SMPI 564


>gb|AFV09099.1| sesquiterpene synthase 2 [Thapsia garganica]
          Length = 561

 Score =  252 bits (644), Expect = 5e-65
 Identities = 132/243 (54%), Positives = 162/243 (66%)
 Frame = +3

Query: 3    DAIEKWDTSCLNMLPEYMQVCYQALLDTYSEMEKVLDKEAGRPTYRVSEAKNSFKRLAKA 182
            DAI+KWD + L+ LPEYM+  Y+ LLD ++E E+ + KE G PTY V  AK +FKRL   
Sbjct: 330  DAIDKWDINILDQLPEYMRHAYKPLLDVFAEGEEEMAKE-GLPTYGVDYAKEAFKRLTVT 388

Query: 183  YLDEAKWYKEGYFPTTVEEYMKVGLVSSGYGTMATHCFVGMGDEATREVFHWVSNDPLIV 362
            YL EAKW +  YFPT  EEYM V LVS     ++   FV MG+ ATRE F W+S DPLIV
Sbjct: 389  YLHEAKWLQAQYFPT-FEEYMSVALVSGAVKMLSVSSFVRMGNIATREAFEWLSKDPLIV 447

Query: 363  RASCLIARLCDDMAGHEFEQEKGDIPSAVECYMKQHCATKEEAIAELQEIVVNAWKDINQ 542
                +I RL DD+ GHEFE ++  IPSAVECYMK H  TKE A AEL++ ++NAWKD+N+
Sbjct: 448  NGLSVICRLSDDIVGHEFENQRPHIPSAVECYMKSHHVTKETAYAELRKPIINAWKDMNE 507

Query: 543  EYYLSCVPAPXXXXXXIIPSSLLARIDNLTRAIHVVYDGDDGYTHSSTRTKDLITSVLID 722
            E      P          P  LL R+ NL R I+ +YDG DGYTHSSTRTKD+ITSVLI+
Sbjct: 508  ECLQPEAP----------PKPLLERVFNLARVINFLYDGHDGYTHSSTRTKDMITSVLIN 557

Query: 723  PFP 731
            P P
Sbjct: 558  PIP 560


>gb|AFV09098.1| sesquiterpene synthase 1 [Thapsia garganica]
          Length = 561

 Score =  244 bits (623), Expect = 1e-62
 Identities = 127/244 (52%), Positives = 160/244 (65%)
 Frame = +3

Query: 3    DAIEKWDTSCLNMLPEYMQVCYQALLDTYSEMEKVLDKEAGRPTYRVSEAKNSFKRLAKA 182
            DAI+KWD S L+ LPEY++  Y+ LLDT++E E+ +  E G PTY V  AK +FKRLA A
Sbjct: 330  DAIDKWDISILDQLPEYVRHAYKPLLDTFAEAEEEMANE-GLPTYGVDYAKEAFKRLAVA 388

Query: 183  YLDEAKWYKEGYFPTTVEEYMKVGLVSSGYGTMATHCFVGMGDEATREVFHWVSNDPLIV 362
            YL E KW +  Y PT  EEY+ V LVS+G   ++   FV MG+ ATRE F W+S DPLI 
Sbjct: 389  YLQETKWLQAQYIPT-FEEYLSVALVSAGGNMLSVSSFVRMGNIATREAFEWLSKDPLIA 447

Query: 363  RASCLIARLCDDMAGHEFEQEKGDIPSAVECYMKQHCATKEEAIAELQEIVVNAWKDINQ 542
                +I RL DD+ GHEFE ++  IPSAVECYMK H  TKE A AELQ+ ++ AWKD+N+
Sbjct: 448  NGLSVIIRLSDDIVGHEFESQRPHIPSAVECYMKSHDVTKETAYAELQKPIIKAWKDMNE 507

Query: 543  EYYLSCVPAPXXXXXXIIPSSLLARIDNLTRAIHVVYDGDDGYTHSSTRTKDLITSVLID 722
                   P          P  LL R+ NL R ++ +YDG DGYTHSSTRTKD+IT+  I+
Sbjct: 508  GCLHPEAP----------PKPLLERVLNLARVLNFLYDGHDGYTHSSTRTKDIITTAFIN 557

Query: 723  PFPL 734
            P PL
Sbjct: 558  PIPL 561


>gb|ABK63808.1| sesquiterpene cyclase [Centella asiatica]
          Length = 563

 Score =  243 bits (621), Expect = 2e-62
 Identities = 119/244 (48%), Positives = 169/244 (69%)
 Frame = +3

Query: 3    DAIEKWDTSCLNMLPEYMQVCYQALLDTYSEMEKVLDKEAGRPTYRVSEAKNSFKRLAKA 182
            DAI++WD + ++ LPEYM+  YQALLD + E+EK +  E GRP +RV  AK+ +K+LA+A
Sbjct: 332  DAIQRWDINAMDQLPEYMKHYYQALLDFFCEIEKYM-VETGRPLFRVQHAKDKYKQLARA 390

Query: 183  YLDEAKWYKEGYFPTTVEEYMKVGLVSSGYGTMATHCFVGMGDEATREVFHWVSNDPLIV 362
            YL EA+W  + Y PT +EEY+   L+++GY  + T  FVGMGD ATRE F WV+NDPL++
Sbjct: 391  YLQEAQWSNKSYVPT-MEEYITNALITTGYTFLGTCSFVGMGDVATREAFDWVTNDPLMI 449

Query: 363  RASCLIARLCDDMAGHEFEQEKGDIPSAVECYMKQHCATKEEAIAELQEIVVNAWKDINQ 542
            RA+ ++ R+CDD+ GHE EQE+  + SA+ECYMKQ+ A+K+E +AE ++ V NAWKD+N+
Sbjct: 450  RAASVVCRICDDIVGHEMEQERQHVASAIECYMKQYGASKDETVAEFKKWVANAWKDLNK 509

Query: 543  EYYLSCVPAPXXXXXXIIPSSLLARIDNLTRAIHVVYDGDDGYTHSSTRTKDLITSVLID 722
            E    C+          +   L  RI N+T  IH +Y   D YT S+  TK++ITSVL+D
Sbjct: 510  E----CL------NPTTMAMPLCTRIFNMTCVIHCLYQQKDAYTFSNNSTKEMITSVLVD 559

Query: 723  PFPL 734
            P P+
Sbjct: 560  PVPI 563