BLASTX nr result
ID: Bupleurum21_contig00025478
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00025478
(1833 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAK50799.1| inwardly rectifying potassium channel subunit [D... 486 e-134
ref|XP_002275549.1| PREDICTED: potassium channel KAT1 [Vitis vin... 438 e-120
gb|AAL24466.1|AF359522_1 inward rectifying potassium channel [Vi... 437 e-120
gb|AAL09479.1|AF359521_1 inward rectifying shaker-like K+ channe... 431 e-118
gb|ABY86891.1| K+ channel protein [Populus euphratica] 421 e-115
>emb|CAK50799.1| inwardly rectifying potassium channel subunit [Daucus carota]
Length = 766
Score = 486 bits (1250), Expect = e-134
Identities = 271/483 (56%), Positives = 343/483 (71%), Gaps = 7/483 (1%)
Frame = -1
Query: 1527 TESLIGNMTNLVVHWSSCTRDFRDTIRAASEFAKRNQLPSCIKNQLLSHICLKFRTEGLK 1348
T LIGNMTNLVVHW+S TRDFRDT+ AASEFAKRN+LP I+NQLLSHICLKFRT+GLK
Sbjct: 288 TSYLIGNMTNLVVHWTSRTRDFRDTVGAASEFAKRNRLPPSIQNQLLSHICLKFRTDGLK 347
Query: 1347 QQGALDNLPKAIRLSIIHHLFMPIIQKARLFRGVSVDYLFQLVTELEAEYYPSKEDVILQ 1168
QQ L +LPKAIR SI HHLF PII+ ARLF+G+S D LFQLVTELEAEY+P KEDVILQ
Sbjct: 348 QQDTLSSLPKAIRSSISHHLFFPIIRNARLFQGLSHDNLFQLVTELEAEYFPPKEDVILQ 407
Query: 1167 NEPPTDLYILVSGAVDLITNINGHDQVLHKAVSGDVFGELGVLCHRPQPFTARTTEVSQI 988
NE P D+YILVSGAVDLI NI+GHDQV+ K G++FGE+GVLCHRPQP+TARTTE+SQI
Sbjct: 408 NEAPMDMYILVSGAVDLIVNIDGHDQVIGKVTEGELFGEVGVLCHRPQPYTARTTEISQI 467
Query: 987 LRLSGPALLGIIEANDEEGQIIMNNFYQEMERLKRDGSKQQKSPRADFGQGLDDKLVGGI 808
LRL+ AL+ II N ++G+IIMNNFY+++E G + K P + + + LD + G
Sbjct: 468 LRLNRNALMNIIHENSDDGRIIMNNFYKDLE---NSGLQSHKRPGSIYSEQLDVRAEGEN 524
Query: 807 HYSTTN-EDVSIENPMPRESTRLEVMEKTTGRTRTFSKYEM-CGNLIKKNQKSLRVALTK 634
+Y D+S E + S E RT S Y M ++ + +Q +L VA+
Sbjct: 525 YYHANQIYDLSGEPLIQGNSVAAE-------NDRTKSGYGMEVKSIAEDDQTALHVAVRT 577
Query: 633 GQPKDTRMCLEEG-SMNKSDDKEWTPAALAKQHGSESIDGLVQSYDINRSKDEHKVDIFP 457
G P++ R+ LE G ++NK D K TP +LA+ G++ I L+ SY RS +E K+++
Sbjct: 578 GHPENVRILLEGGANVNKLDAKGRTPISLAENQGNKCIYDLLLSYQNTRSTNEQKIELLE 637
Query: 456 ET---TKSDRFKPTPNGPIGYFDSYLKDYVLFGSSSLSHPEDAQVVKINKRRVTIHMKFL 286
E+ T++ +FK T G SY KD + S +L+H +A+V KIN RVTIHM
Sbjct: 638 ESSYETRNKQFKETHTGVTTCSSSYQKDSLCSSSEALNHSAEAEVRKINTIRVTIHM--- 694
Query: 285 KSNASPKQLGKLIMLPDSMEELFRIAGQKFGGY-FTKVVNSEDAEIDDLHLIRDGDHLFL 109
+NAS KQL KLI LP S++ELFRIAGQK+GGY FT++VNSE+AEIDDL +IRDGDHLFL
Sbjct: 695 -NNASQKQLAKLINLPGSIDELFRIAGQKYGGYNFTEMVNSENAEIDDLSVIRDGDHLFL 753
Query: 108 VPE 100
+PE
Sbjct: 754 IPE 756
Score = 67.8 bits (164), Expect = 9e-09
Identities = 33/57 (57%), Positives = 42/57 (73%)
Frame = -2
Query: 1688 MSSSCTKNFLQKFCVNEFQMDKGYKRGFFSDDLLPSLGASIDHATILPKYIISPLNP 1518
MS + TKNFL++FCV++FQ G + FFS+ LLPSLGA+I+ T L K IISP NP
Sbjct: 1 MSFTSTKNFLRRFCVDDFQTRTGAQSSFFSNGLLPSLGANINQGTKLQKNIISPFNP 57
>ref|XP_002275549.1| PREDICTED: potassium channel KAT1 [Vitis vinifera]
Length = 791
Score = 438 bits (1126), Expect = e-120
Identities = 245/492 (49%), Positives = 331/492 (67%), Gaps = 15/492 (3%)
Frame = -1
Query: 1527 TESLIGNMTNLVVHWSSCTRDFRDTIRAASEFAKRNQLPSCIKNQLLSHICLKFRTEGLK 1348
T LIGNMTNLVVHW+S TRDFRDT+R+ASEFA RNQLP I++Q+LSH+CLKF+TEGLK
Sbjct: 288 TSYLIGNMTNLVVHWTSRTRDFRDTVRSASEFATRNQLPPRIQDQMLSHLCLKFKTEGLK 347
Query: 1347 QQGALDNLPKAIRLSIIHHLFMPIIQKARLFRGVSVDYLFQLVTELEAEYYPSKEDVILQ 1168
QQ L+ LP+AIR SI H+LF PI Q LF+GVS D+LFQLV+E+EAEY+P +EDVILQ
Sbjct: 348 QQDTLNGLPRAIRSSIAHYLFFPIAQNVYLFQGVSQDFLFQLVSEVEAEYFPPREDVILQ 407
Query: 1167 NEPPTDLYILVSGAVDLITNINGHDQVLHKAVSGDVFGELGVLCHRPQPFTARTTEVSQI 988
E PTD+YILVSGAVDLI I+GHDQ+L KAV+GDVFGE+GVLC+RPQ T RT+E+SQI
Sbjct: 408 KEAPTDIYILVSGAVDLIAYIDGHDQILGKAVAGDVFGEIGVLCYRPQSLTVRTSELSQI 467
Query: 987 LRLSGPALLGIIEANDEEGQIIMNNFYQEMERLKRDG-SKQQKSPRADFGQGLDDKLVGG 811
LRLS +L+ I+AN E+GQIIMNN +++++ L+ G + P + + +D GG
Sbjct: 468 LRLSRTSLMNAIQANMEDGQIIMNNLFKKLKGLESSGFTDPHMDPESILREWIDGVPPGG 527
Query: 810 -IHYSTTNEDVSIENPMPRESTRLEVM-EKTTGRTRTFSKYEMCGNLIKKN------QKS 655
+ ++ ++ +P +E+ + ++ + T +++ +E G I N Q +
Sbjct: 528 SLSHAGCHDQSPHGDPSIQEARDIGLLGSEATKKSKADKAHESTGCGIDANSAAEDGQTA 587
Query: 654 LRVALTKGQPKDTRMCLEEG-SMNKSDDKEWTPAALAKQHGSESIDGLVQSYDINRSKDE 478
L VA+ G + R+ LE G ++NK D + WTP ALA+Q G +SI L+ SY+ R DE
Sbjct: 588 LHVAVCNGHLEMVRILLERGANVNKKDARGWTPKALAEQEGKKSIYDLLLSYENRRLLDE 647
Query: 477 HKVDIF----PETTKSDRFKPTPNGPIGYFDSYLKDYVLFGSSSLSHPEDAQVVKINKRR 310
HK+ + S GP + + K S S S P + V+ + KRR
Sbjct: 648 HKIHFIGSGAADCCTSQGLHTRTGGPNFHNSQFKKVSTNSNSGSPSPPGNKDVMTLTKRR 707
Query: 309 VTIHMKFLKSNASPKQLGKLIMLPDSMEELFRIAGQKFGGYF-TKVVNSEDAEIDDLHLI 133
VTIH +F ++ S Q GKLI+LPDS+EEL +IAGQKFGGY TKVV++ +AEIDD+ +I
Sbjct: 708 VTIHRQFQNASTSQGQFGKLIILPDSIEELLQIAGQKFGGYNPTKVVSAGNAEIDDISVI 767
Query: 132 RDGDHLFLVPED 97
RDGDHLFL+ +
Sbjct: 768 RDGDHLFLLQNE 779
Score = 67.0 bits (162), Expect = 2e-08
Identities = 35/66 (53%), Positives = 43/66 (65%)
Frame = -2
Query: 1688 MSSSCTKNFLQKFCVNEFQMDKGYKRGFFSDDLLPSLGASIDHATILPKYIISPLNPLLG 1509
MS SC K F Q+FCV EFQM++ FS LLPSLGA I+ AT L K+IISP +P
Sbjct: 1 MSFSCAKAFFQRFCVEEFQMERTSLGSVFSSHLLPSLGARINQATKLQKHIISPFSPRYR 60
Query: 1508 T*QILL 1491
++LL
Sbjct: 61 AWEMLL 66
>gb|AAL24466.1|AF359522_1 inward rectifying potassium channel [Vitis vinifera]
Length = 791
Score = 437 bits (1123), Expect = e-120
Identities = 244/492 (49%), Positives = 330/492 (67%), Gaps = 15/492 (3%)
Frame = -1
Query: 1527 TESLIGNMTNLVVHWSSCTRDFRDTIRAASEFAKRNQLPSCIKNQLLSHICLKFRTEGLK 1348
T LIGNMTNLVVHW+S TRDFRDT+R+ASEFA RNQLP I++Q+LSH+CLKF+TEGLK
Sbjct: 288 TSYLIGNMTNLVVHWTSRTRDFRDTVRSASEFATRNQLPPRIQDQMLSHLCLKFKTEGLK 347
Query: 1347 QQGALDNLPKAIRLSIIHHLFMPIIQKARLFRGVSVDYLFQLVTELEAEYYPSKEDVILQ 1168
QQ L+ LP+AIR SI H+LF PI Q LF+GVS D+LFQLV+E+EAEY+P +EDVILQ
Sbjct: 348 QQDTLNGLPRAIRSSIAHYLFFPIAQNVYLFQGVSQDFLFQLVSEVEAEYFPPREDVILQ 407
Query: 1167 NEPPTDLYILVSGAVDLITNINGHDQVLHKAVSGDVFGELGVLCHRPQPFTARTTEVSQI 988
E PTD+YILVSGAVDLI I+GHDQ+L KAV+GDVFGE+GVLC+RPQ T RT+E+SQI
Sbjct: 408 KEAPTDIYILVSGAVDLIAYIDGHDQILGKAVAGDVFGEIGVLCYRPQSLTVRTSELSQI 467
Query: 987 LRLSGPALLGIIEANDEEGQIIMNNFYQEMERLKRDG-SKQQKSPRADFGQGLDDKLVGG 811
LRLS +L+ I AN E+G IIMNN +++++ L+ G + P + + +D GG
Sbjct: 468 LRLSRTSLMNAIRANMEDGHIIMNNLFKKLKGLESSGFTDPHMDPESILREWIDGVPPGG 527
Query: 810 -IHYSTTNEDVSIENPMPRESTRLEVM-EKTTGRTRTFSKYEMCGNLIKKN------QKS 655
+ ++ ++ +P +E+ ++++ + T +++ +E G I N Q +
Sbjct: 528 SLSHAGCHDQSPHGDPSIQEARDIDLLGSEATKKSKADKAHESTGCGIDANSAAEDGQTA 587
Query: 654 LRVALTKGQPKDTRMCLEEG-SMNKSDDKEWTPAALAKQHGSESIDGLVQSYDINRSKDE 478
L VA+ G + R+ LE G ++NK D + WTP ALA+Q G +SI L+ SY+ R DE
Sbjct: 588 LHVAVCNGHLEMVRILLERGANVNKKDARGWTPKALAEQEGKKSIYDLLLSYENRRLLDE 647
Query: 477 HKVDIFPETTK----SDRFKPTPNGPIGYFDSYLKDYVLFGSSSLSHPEDAQVVKINKRR 310
HK+ + S GP + + K S S S P + V+ + KRR
Sbjct: 648 HKIHFIGSGARDCCTSQGLHTRTGGPNFHNSQFKKVSTNSNSGSPSPPGNKDVMTLTKRR 707
Query: 309 VTIHMKFLKSNASPKQLGKLIMLPDSMEELFRIAGQKFGGYF-TKVVNSEDAEIDDLHLI 133
VTIH +F ++ S Q GKLI+LPDS+EEL +IAGQKFGGY TKVV++ +AEIDD+ +I
Sbjct: 708 VTIHRQFQNASTSQGQFGKLIILPDSIEELLQIAGQKFGGYNPTKVVSAGNAEIDDISVI 767
Query: 132 RDGDHLFLVPED 97
RDGDHLFL+ +
Sbjct: 768 RDGDHLFLLQNE 779
Score = 65.5 bits (158), Expect = 5e-08
Identities = 34/66 (51%), Positives = 42/66 (63%)
Frame = -2
Query: 1688 MSSSCTKNFLQKFCVNEFQMDKGYKRGFFSDDLLPSLGASIDHATILPKYIISPLNPLLG 1509
MS SC K F Q+FCV EFQM++ FS LLPSLG I+ AT L K+IISP +P
Sbjct: 1 MSFSCAKAFFQRFCVEEFQMERTSLGSVFSSHLLPSLGGRINQATKLQKHIISPFSPRYR 60
Query: 1508 T*QILL 1491
++LL
Sbjct: 61 AWEMLL 66
>gb|AAL09479.1|AF359521_1 inward rectifying shaker-like K+ channel [Vitis vinifera]
Length = 791
Score = 431 bits (1109), Expect = e-118
Identities = 243/492 (49%), Positives = 330/492 (67%), Gaps = 15/492 (3%)
Frame = -1
Query: 1527 TESLIGNMTNLVVHWSSCTRDFRDTIRAASEFAKRNQLPSCIKNQLLSHICLKFRTEGLK 1348
T LIGNMTNLVVHW+S TRDFRDT+R+ASEFA RNQLP I++Q+LSH+CLKF+TEGLK
Sbjct: 288 TSYLIGNMTNLVVHWTSRTRDFRDTVRSASEFATRNQLPPRIQDQMLSHLCLKFKTEGLK 347
Query: 1347 QQGALDNLPKAIRLSIIHHLFMPIIQKARLFRGVSVDYLFQLVTELEAEYYPSKEDVILQ 1168
QQ L+ LP+AIR SI H+LF PI Q LF+GVS D+LFQLV+E+EAEY+P +EDVILQ
Sbjct: 348 QQDTLNGLPRAIRSSIAHYLFFPIAQNVYLFQGVSQDFLFQLVSEVEAEYFPPREDVILQ 407
Query: 1167 NEPPTDLYILVSGAVDLITNINGHDQVLHKAVSGDVFGELGVLCHRPQPFTARTTEVSQI 988
E TD+YILVSGAVDLI I+GHDQ+L KAV+GDVFGE+GVLC+RPQ T RT+E+SQI
Sbjct: 408 KEASTDIYILVSGAVDLIAYIDGHDQILGKAVAGDVFGEIGVLCYRPQSLTVRTSELSQI 467
Query: 987 LRLSGPALLGIIEANDEEGQIIMNNFYQEMERLKRDG-SKQQKSPRADFGQGLDDKLVGG 811
LRLS +L+ I+AN E+G IIMN+ +++++ L+ G + P + + +D GG
Sbjct: 468 LRLSRTSLMNAIQANMEDGPIIMNHLFKKLKGLESSGFTDPHMDPDSILREWIDGVPPGG 527
Query: 810 -IHYSTTNEDVSIENPMPRESTRLEVM-EKTTGRTRTFSKYEMCGNLIKKN------QKS 655
+ ++ ++ +P +E+ + ++ + T +++ +E G I N Q +
Sbjct: 528 SLSHAGCHDQSPHGDPSIQEARDIGLLGSEATKKSKADKAHESTGCGIDANSAAEDGQTA 587
Query: 654 LRVALTKGQPKDTRMCLEEG-SMNKSDDKEWTPAALAKQHGSESIDGLVQSYDINRSKDE 478
L VA+ G + R+ LE G ++NK D + WTP ALA+Q G +SI L+ SY+ R DE
Sbjct: 588 LHVAVCNGHLEMVRILLERGANVNKKDARGWTPKALAEQEGKKSIYDLLLSYENRRLLDE 647
Query: 477 HKVDIF----PETTKSDRFKPTPNGPIGYFDSYLKDYVLFGSSSLSHPEDAQVVKINKRR 310
HK+ + S GP + + K S S S P + V+ + KRR
Sbjct: 648 HKIHFIGSDAADCCTSQGLHTRTGGPNFHNSQFKKVSTNSNSGSPSPPGNKDVMTLTKRR 707
Query: 309 VTIHMKFLKSNASPKQLGKLIMLPDSMEELFRIAGQKFGGYF-TKVVNSEDAEIDDLHLI 133
VTIH +F ++ S QLGKLI+LPDS+EEL +IAGQKFGGY TKVV++ +AEIDD+ +I
Sbjct: 708 VTIHRQFQNASTSQGQLGKLIILPDSIEELLQIAGQKFGGYNPTKVVSAGNAEIDDISVI 767
Query: 132 RDGDHLFLVPED 97
RDGDHLFL+ +
Sbjct: 768 RDGDHLFLLQNE 779
Score = 67.0 bits (162), Expect = 2e-08
Identities = 35/66 (53%), Positives = 43/66 (65%)
Frame = -2
Query: 1688 MSSSCTKNFLQKFCVNEFQMDKGYKRGFFSDDLLPSLGASIDHATILPKYIISPLNPLLG 1509
MS SC K F Q+FCV EFQM++ FS LLPSLGA I+ AT L K+IISP +P
Sbjct: 1 MSFSCAKAFFQRFCVEEFQMERTSLGSVFSSHLLPSLGARINQATKLQKHIISPFSPRYR 60
Query: 1508 T*QILL 1491
++LL
Sbjct: 61 AWEMLL 66
>gb|ABY86891.1| K+ channel protein [Populus euphratica]
Length = 746
Score = 421 bits (1081), Expect = e-115
Identities = 237/481 (49%), Positives = 318/481 (66%), Gaps = 4/481 (0%)
Frame = -1
Query: 1527 TESLIGNMTNLVVHWSSCTRDFRDTIRAASEFAKRNQLPSCIKNQLLSHICLKFRTEGLK 1348
T LIGNMTNLVVHW+S TR+FRDT+RAASEFA RNQLP I+ Q+LSHICLKF+TEGLK
Sbjct: 288 TSYLIGNMTNLVVHWTSRTRNFRDTVRAASEFAARNQLPPRIQEQMLSHICLKFKTEGLK 347
Query: 1347 QQGALDNLPKAIRLSIIHHLFMPIIQKARLFRGVSVDYLFQLVTELEAEYYPSKEDVILQ 1168
QQ L+ LPKAIR SI +LF PI Q A LF+GVS D+LFQLV+E+EAEY+P KEDVILQ
Sbjct: 348 QQETLNGLPKAIRSSIADYLFHPIAQSAYLFQGVSQDFLFQLVSEMEAEYFPPKEDVILQ 407
Query: 1167 NEPPTDLYILVSGAVDLITNINGHDQVLHKAVSGDVFGELGVLCHRPQPFTARTTEVSQI 988
NE PTDLYILVSG VDLI +++ ++V+ KA++GD FGE+GVLC RPQPFT RT E+SQI
Sbjct: 408 NEAPTDLYILVSGTVDLILHVDEREKVIGKAIAGDTFGEVGVLCSRPQPFTVRTIELSQI 467
Query: 987 LRLSGPALLGIIEANDEEGQIIMNNFYQEMERLKRDGSKQQKSPRADFGQGLDDKLVGGI 808
LRL+G AL+ I+AN E+G++IMN+ ++ R + S+ Q +G D + G
Sbjct: 468 LRLNGTALMSTIKANPEDGRVIMNHLSMKLRRRESMDSESQYREEWCSKRGCKDHMHG-- 525
Query: 807 HYSTTNEDVSIENPMPRESTRLEVMEKTTGRTRTFSKYEMCGNLIKKNQKSLRVALTKGQ 628
D+S+ +S K T ++ S++E ++ ++ +L A+ +G
Sbjct: 526 -------DLSVNKARETDSQG----SKATRKSELGSRHEGLVTAVENSETALHAAVCEGH 574
Query: 627 PKDTRMCLEEG-SMNKSDDKEWTPAALAKQHGSESIDGLVQSYDINRSKDEHKVDIFPET 451
+ ++ L+ G S+NK D + WTP ALA+Q G++SI L+ +Y+ +EH++D
Sbjct: 575 VEMVKILLDGGASINKPDARGWTPKALAEQQGNKSIHDLLLNYENRNILNEHRIDFIESE 634
Query: 450 TKSDRFKPTPNGPIGYFDSYLKDYVLFGSSSLSH--PEDAQVVKINKRRVTIHMKFLKSN 277
T D K + + L SSS P D + K K RVTIHM+ +
Sbjct: 635 TVGDTKKSQ--------GKHEGNKALTNSSSCISRCPLDREAKKSTK-RVTIHMQLQNRS 685
Query: 276 ASPKQLGKLIMLPDSMEELFRIAGQKFGGY-FTKVVNSEDAEIDDLHLIRDGDHLFLVPE 100
+LGKLI+LPDSMEEL RIAG+KFGGY FT+V+N+E+AEID + +IRDGDHLFL+ +
Sbjct: 686 TLQSRLGKLIILPDSMEELLRIAGEKFGGYKFTRVMNAENAEIDGISVIRDGDHLFLLQD 745
Query: 99 D 97
D
Sbjct: 746 D 746