BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00025382
         (937 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEO19902.1| chlorophyllase 1 [Pyrus x bretschneideri]              239   9e-61
ref|XP_002271974.1| PREDICTED: chlorophyllase-1 [Vitis vinifera]      237   3e-60
ref|XP_003528958.1| PREDICTED: chlorophyllase-1-like [Glycine ma...   236   6e-60
emb|CBI28741.3| unnamed protein product [Vitis vinifera]              236   8e-60
ref|XP_002273926.1| PREDICTED: chlorophyllase-1 [Vitis vinifera]      236   8e-60

>gb|AEO19902.1| chlorophyllase 1 [Pyrus x bretschneideri]
          Length = 302

 Score =  239 bits (609), Expect = 9e-61
 Identities = 134/270 (49%), Positives = 168/270 (62%), Gaps = 10/270 (3%)
 Frame = -2

Query: 783 SSKQKELFIVFPANEGKYPVFLFIHGCCISNTTYSRLLQHVASHGFIAVAPGDPLLSSLL 604
           S+  K LFIV P   G YPV L  HG  + N  Y  LLQH+ SHGFIAVAP    L   +
Sbjct: 22  SAPPKPLFIVTPTVAGTYPVILLHHGFYLRNYFYEELLQHIVSHGFIAVAPQ---LCGAV 78

Query: 603 CCCGNQELESDTNVLEWVPDGLPTVLPDKVIADTTKLAVGGHSRGGKIAFALAL----AN 436
              G +E+ES   V+ W+P GL  +LP+ V+AD TK A+ GHSRGGK AFA+AL     +
Sbjct: 79  PPSGPEEVESGAKVINWLPKGLQPLLPENVVADLTKFALSGHSRGGKTAFAIALGRAKTS 138

Query: 435 TSPKVSAVFGVDPVAGGSTDIRPQPKIFHYIPRCFDIKVPVAIIGTGLGCQSK--CMIPP 262
            S K+S + G+DPVAG +   R +P I  Y P+ FD+ +PV +IGTGLG +SK  CM  P
Sbjct: 139 LSLKISVLIGIDPVAGANRYCRTRPHILTYSPQSFDLSIPVTVIGTGLGPESKNACMSQP 198

Query: 261 FAPYGVNHAEFFNESKPPVYYFLAKDYGHGDMLDD---GLEYWFTKLMTKHGKGPKEFMI 91
            AP GVNH EFF E KPP  +F+ KDYGH  MLDD   G+    +  M K+G GP+E M 
Sbjct: 199 CAPDGVNHKEFFYECKPPCAHFVVKDYGHMGMLDDDPQGVVGAVSGCMCKNGTGPREIMR 258

Query: 90  KACGGIIVAFLNAKLK-DEVKDLEIIHQPN 4
           K  GGI+VAFLNA L  D+   L I+  P+
Sbjct: 259 KTVGGIVVAFLNAYLNGDDRYLLAIVGDPD 288


>ref|XP_002271974.1| PREDICTED: chlorophyllase-1 [Vitis vinifera]
          Length = 319

 Score =  237 bits (604), Expect = 3e-60
 Identities = 141/300 (47%), Positives = 187/300 (62%), Gaps = 10/300 (3%)
 Frame = -2

Query: 870 MDLKPALATSVPSVFEKGNLKVKEIKVKRS---SKQKELFIVFPANEGKYPVFLFIHGCC 700
           +DLK     +  SVFE GNL V  I+V+ S   S  K L IV P  +G YPV LF+HG  
Sbjct: 15  LDLK-----TTTSVFEPGNLSVSCIRVETSNIASPPKPLLIVTPTIQGTYPVLLFLHGFE 69

Query: 699 ISNTTYSRLLQHVASHGFIAVAPGDPLLSSLLCCCGNQELESDTNVLEWVPDGLPTVLPD 520
           + NT Y++LLQ ++SHGFI VAP    L  LL   G QE++S   V  W+  GL +VLP+
Sbjct: 70  LRNTFYTQLLQLISSHGFIVVAPQ---LYGLLPPSGIQEIKSAAAVTNWLSSGLQSVLPE 126

Query: 519 KVIADTTKLAVGGHSRGGKIAFALAL--ANTSPKVSAVFGVDPVAGGSTDIRPQPKIFHY 346
            V  D  KLA+ GHSRGGK AFALAL  A+TS   SA+ G+DPV G S   +  PKI  Y
Sbjct: 127 NVKPDLLKLALSGHSRGGKTAFALALGYADTSLNFSALLGLDPVGGLSKCCQTVPKILTY 186

Query: 345 IPRCFDIKVPVAIIGTGLGCQSK-CMIPPFAPYGVNHAEFFNESKPPVYYFLAKDYGHGD 169
           +P  F++ +PV +IGTGLG + + C+  P AP GVNH EFF+E KPP  +F+  +YGH D
Sbjct: 187 VPHSFNLAIPVCVIGTGLGDEPRNCLTCPCAPDGVNHVEFFSECKPPCSHFVTTEYGHLD 246

Query: 168 MLDDGLEYWFTKL---MTKHGKGPKEFMIKACGGIIVAFLNAKLKDEVKDLE-IIHQPNI 1
           MLDD L      +   + K GKGP++ M +  GG+ VAFL A L+ +  D + I+ +P++
Sbjct: 247 MLDDHLSGCIGAISGYICKSGKGPRDPMRRCVGGLFVAFLKAYLEGQSGDFKAIVDEPDL 306


>ref|XP_003528958.1| PREDICTED: chlorophyllase-1-like [Glycine max]
           gi|121308623|dbj|BAF43704.1| Chlorophyllase 2 [Glycine
           max]
          Length = 316

 Score =  236 bits (602), Expect = 6e-60
 Identities = 139/302 (46%), Positives = 189/302 (62%), Gaps = 15/302 (4%)
 Frame = -2

Query: 861 KPALATSVPSVFEKGNLKVKEIKVKRS----SKQKELFIVFPANEGKYPVFLFIHGCCIS 694
           +PALAT+   VF+KG++  K+  V+ S    S  K L I  P   G YPV LF HG CI 
Sbjct: 6   QPALATT--DVFQKGDIHWKQFNVETSTASSSPPKPLLIFTPTVPGLYPVILFCHGFCIR 63

Query: 693 NTTYSRLLQHVASHGFIAVAPGDPLLSSLLCCCGNQELESDTNVLEWVPDGLPTVLPDKV 514
            + YS+LL H+ SHGFI VAP   L S  +   G +E++ +  V++W+ +GL  +LP+ V
Sbjct: 64  TSYYSKLLAHIVSHGFILVAP--QLFSIGVPMFGPEEVKCEGRVVDWLDNGLQPLLPESV 121

Query: 513 IADTTKLAVGGHSRGGKIAFALALA--NTSPKVSAVFGVDPVAGGST--DIRPQPKIFHY 346
            A   KL + GHS+GGK AFA+AL    T  K SA+ G+DPVAG S     R  P I   
Sbjct: 122 EAKLEKLVLVGHSKGGKTAFAVALGYCKTKLKFSALIGIDPVAGVSKCKPCRSLPDILTG 181

Query: 345 IPRCFDIKVPVAIIGTGLGCQ-SKCMIPPFAPYGVNHAEFFNESKPPVYYFLAKDYGHGD 169
           +PR F++ +PVA+IGTGLG + +  + PP AP GVNH EFF+E KPP  YF+A DYGH D
Sbjct: 182 VPRSFNLNIPVAVIGTGLGPEKANSLFPPCAPNGVNHKEFFSECKPPSAYFVATDYGHMD 241

Query: 168 MLDDG----LEYWFTKLMTKHG-KGPKEFMIKACGGIIVAFLNAKLKDEVKDLE-IIHQP 7
           MLDD     +    +K M K+G KGP++ M +  GG++VAFL A+L ++ KD + I+  P
Sbjct: 242 MLDDETPGVIGTMMSKCMCKNGKKGPRDLMRRTVGGLVVAFLRAQLNEQWKDFDAILASP 301

Query: 6   NI 1
           N+
Sbjct: 302 NL 303


>emb|CBI28741.3| unnamed protein product [Vitis vinifera]
          Length = 881

 Score =  236 bits (601), Expect = 8e-60
 Identities = 140/300 (46%), Positives = 187/300 (62%), Gaps = 10/300 (3%)
 Frame = -2

Query: 870  MDLKPALATSVPSVFEKGNLKVKEIKVKRS---SKQKELFIVFPANEGKYPVFLFIHGCC 700
            +DLK     +  SVFE GNL V  I+V+ S   S  K L IV P  +G YPV LF+HG  
Sbjct: 267  LDLK-----TTTSVFEPGNLSVTCIRVETSNIASPPKPLLIVTPTIQGTYPVLLFLHGFE 321

Query: 699  ISNTTYSRLLQHVASHGFIAVAPGDPLLSSLLCCCGNQELESDTNVLEWVPDGLPTVLPD 520
            + NT Y++LLQ ++SHG+I VAP    L  LL   G QE++S   V  W+  GL +VLP+
Sbjct: 322  LRNTFYTQLLQLISSHGYIVVAPQ---LYGLLPPSGIQEIKSAAAVTNWLSSGLQSVLPE 378

Query: 519  KVIADTTKLAVGGHSRGGKIAFALAL--ANTSPKVSAVFGVDPVAGGSTDIRPQPKIFHY 346
             V  D  KLA+ GHSRGGK AFALAL  A+TS   SA+ G+DPV G S   +  PKI  Y
Sbjct: 379  NVKPDLLKLALSGHSRGGKTAFALALGYADTSLNFSALLGLDPVGGLSKCSQTVPKILTY 438

Query: 345  IPRCFDIKVPVAIIGTGLGCQSK-CMIPPFAPYGVNHAEFFNESKPPVYYFLAKDYGHGD 169
            +P  F++ +PV +IGTGLG + + C+  P AP GVNH EFF+E KPP  +F+  +YGH D
Sbjct: 439  VPHSFNLAIPVCVIGTGLGDEPRNCLTCPCAPDGVNHVEFFSECKPPCSHFVTTEYGHLD 498

Query: 168  MLDDGLEYWFTKL---MTKHGKGPKEFMIKACGGIIVAFLNAKLKDEVKDLE-IIHQPNI 1
            MLDD L      +   + K GKGP++ M +  GG+ VAFL A L+ +  D + I+ +P++
Sbjct: 499  MLDDHLSGCIGAISGYICKSGKGPRDPMRRCVGGLFVAFLKAYLEGQTGDFKAIVDEPDL 558



 Score =  211 bits (538), Expect = 2e-52
 Identities = 130/309 (42%), Positives = 178/309 (57%), Gaps = 17/309 (5%)
 Frame = -2

Query: 876  AVMDLKPALATSVPSVFEKGNLKVKEIKVKRSSKQKELFIVFPANEGKYPVFLFIHGCCI 697
            A ++ +PA A +   VF++GN   K I     +  K LFIV P  EG YPV LF+HG  I
Sbjct: 568  AALEARPAPAAAT-DVFKRGNYTAKPITT--CNAPKTLFIVTPDTEGTYPVLLFLHGYNI 624

Query: 696  SNTTYSRLLQHVASHGFIAVAPGDPLLSSLLCCCGNQELESDTNVLEWVPDGLPTVLPDK 517
                Y+ LL+H++SHG I VAP    L SL    G  ++ S   V  W+  GL +VLP+ 
Sbjct: 625  CPCCYTNLLEHISSHGDIVVAPR---LLSLCSLYGRPDINSAAEVANWLSSGLQSVLPEN 681

Query: 516  VIADTTKLAVGGHSRGGKIAFALALANTSPKVSAVF----GVDPVAGGSTDIRPQPKIFH 349
            V+ D ++LA+ GHSRGG +AFALAL N +  ++  F    G+DPVAG +  ++  PKI  
Sbjct: 682  VVPDLSRLALAGHSRGGYLAFALALGNANVSMNLKFLTLIGIDPVAGANKCMKMCPKILT 741

Query: 348  YIPRCFDIKVPVAIIGTGLGCQS--KCMIPPFAPYGVNHAEFFNESKPPVYYFLAKDYGH 175
             +P  F + +PV +IGTGLG +S   C+    AP G+N+AEFFNE K     F+  DYGH
Sbjct: 742  GVPHSFILDIPVMVIGTGLGGESVIGCIPCSCAPDGLNYAEFFNECKDNCLGFVIPDYGH 801

Query: 174  GDMLDDGLEYWFTKL----------MTKHGKGPKEFMIKACGGIIVAFLNAKLKDEVKDL 25
             DMLDD  +Y    +          M K GKG K  M++  GG++VA L A L+ E  DL
Sbjct: 802  MDMLDD--DYCTNCIGTSIGAIMGSMCKSGKGDKTSMMECVGGLVVAMLMAHLEGETGDL 859

Query: 24   E-IIHQPNI 1
            + I+ +P I
Sbjct: 860  DAIVDEPGI 868


>ref|XP_002273926.1| PREDICTED: chlorophyllase-1 [Vitis vinifera]
          Length = 319

 Score =  236 bits (601), Expect = 8e-60
 Identities = 140/300 (46%), Positives = 187/300 (62%), Gaps = 10/300 (3%)
 Frame = -2

Query: 870 MDLKPALATSVPSVFEKGNLKVKEIKVKRS---SKQKELFIVFPANEGKYPVFLFIHGCC 700
           +DLK     +  SVFE GNL V  I+V+ S   S  K L IV P  +G YPV LF+HG  
Sbjct: 15  LDLK-----TTTSVFEPGNLSVTCIRVETSNIASPPKPLLIVTPTIQGTYPVLLFLHGFE 69

Query: 699 ISNTTYSRLLQHVASHGFIAVAPGDPLLSSLLCCCGNQELESDTNVLEWVPDGLPTVLPD 520
           + NT Y++LLQ ++SHG+I VAP    L  LL   G QE++S   V  W+  GL +VLP+
Sbjct: 70  LRNTFYTQLLQLISSHGYIVVAPQ---LYGLLPPSGIQEIKSAAAVTNWLSSGLQSVLPE 126

Query: 519 KVIADTTKLAVGGHSRGGKIAFALAL--ANTSPKVSAVFGVDPVAGGSTDIRPQPKIFHY 346
            V  D  KLA+ GHSRGGK AFALAL  A+TS   SA+ G+DPV G S   +  PKI  Y
Sbjct: 127 NVKPDLLKLALSGHSRGGKTAFALALGYADTSLNFSALLGLDPVGGLSKCSQTVPKILTY 186

Query: 345 IPRCFDIKVPVAIIGTGLGCQSK-CMIPPFAPYGVNHAEFFNESKPPVYYFLAKDYGHGD 169
           +P  F++ +PV +IGTGLG + + C+  P AP GVNH EFF+E KPP  +F+  +YGH D
Sbjct: 187 VPHSFNLAIPVCVIGTGLGDEPRNCLTCPCAPDGVNHVEFFSECKPPCSHFVTTEYGHLD 246

Query: 168 MLDDGLEYWFTKL---MTKHGKGPKEFMIKACGGIIVAFLNAKLKDEVKDLE-IIHQPNI 1
           MLDD L      +   + K GKGP++ M +  GG+ VAFL A L+ +  D + I+ +P++
Sbjct: 247 MLDDHLSGCIGAISGYICKSGKGPRDPMRRCVGGLFVAFLKAYLEGQTGDFKAIVDEPDL 306