BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00025366
         (1552 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003522088.1| PREDICTED: mannan endo-1,4-beta-mannosidase ...   714   0.0  
gb|AFX59322.1| endo-1,4-beta-mannanase [Populus trichocarpa]          710   0.0  
ref|XP_002309191.1| predicted protein [Populus trichocarpa] gi|2...   706   0.0  
emb|CBI18019.3| unnamed protein product [Vitis vinifera]              706   0.0  
ref|XP_002273389.1| PREDICTED: mannan endo-1,4-beta-mannosidase ...   704   0.0  

>ref|XP_003522088.1| PREDICTED: mannan endo-1,4-beta-mannosidase 6-like [Glycine max]
          Length = 438

 Score =  714 bits (1842), Expect = 0.0
 Identities = 328/412 (79%), Positives = 372/412 (90%)
 Frame = +2

Query: 38   SEMADMEDNAWAMVKKQGNQFVVNNQPFYVNGFNTYWLMVFAVDQSTRAKVSEVFQQASS 217
            SE+ DME+N W MV+ +GNQFVVN+QPFYVNGFNTYWLMVFA D+STR KV+EVF+ ASS
Sbjct: 27   SELGDMEENEWQMVQTKGNQFVVNDQPFYVNGFNTYWLMVFAADESTRGKVTEVFKHASS 86

Query: 218  VGLTICRTWAFNDGQWRALQKSPSVYDEDVFKALDFVISEAKKYKIRLILSLTNNWDAYG 397
            VG+++CRTWAFNDGQWRALQKSPSVYDEDVFKALDFV+SEA+KYKIRLILSL NNW+AYG
Sbjct: 87   VGMSVCRTWAFNDGQWRALQKSPSVYDEDVFKALDFVVSEARKYKIRLILSLVNNWEAYG 146

Query: 398  GKPQYVKWGKDAGLNLTSEDEFFSHPTLRRYYKAHVKTVLNRVNTVNNITYKDDPTIFAW 577
            GKPQYVKWG  AGLNLTS+D+FFSHPTLR YYKAHVKTVLNRVNT  NITYK+DPTIFAW
Sbjct: 147  GKPQYVKWGNAAGLNLTSDDDFFSHPTLRSYYKAHVKTVLNRVNTFTNITYKEDPTIFAW 206

Query: 578  ELMNEPRCPSDSSGNTLQEWIEEMAVYVKTIDPNHLVEIGLEGFYGPSTPNRVQFNPNTY 757
            ELMNEPRC SDSSG+ LQ+WI+EMA YVK+IDP HLVEIGLEGFYGPSTP + QFNPN+Y
Sbjct: 207  ELMNEPRCTSDSSGDVLQDWIKEMAFYVKSIDPKHLVEIGLEGFYGPSTPQKYQFNPNSY 266

Query: 758  AQQIGTDFIRNHNILGVDFASVHIYADSWISQLVDDSHLKFIKSWMEAHIEDAENVLGMP 937
            A ++GTDFIRNH +LGVDFASVHIY DSWISQ V DSHL FIK+WMEAHIEDAE  LGMP
Sbjct: 267  AHEVGTDFIRNHQVLGVDFASVHIYPDSWISQSVADSHLPFIKTWMEAHIEDAEKYLGMP 326

Query: 938  VIFGEFGVSTKDPGYNSSYRDKVLSTVYNTLLNSTKKGGSGAGALIWQVFPEGTDYMDDG 1117
            V+F EFGVS K PGYNS+YRD +++TVY T+LNSTKKGGSGAG+L+WQ+FP+GTDYMDDG
Sbjct: 327  VVFAEFGVSAKAPGYNSTYRDNLINTVYKTILNSTKKGGSGAGSLVWQLFPDGTDYMDDG 386

Query: 1118 YAIVLSKSPSTSNIVSLHSKRLAMFNSLCSWRCHWGCKKKHKLEDEFFHDEL 1273
            YAIVLSKSPSTS+I+SL S RLA+FNSLCS +C WGCKK++ LE   +HDEL
Sbjct: 387  YAIVLSKSPSTSSIISLQSTRLALFNSLCSKKCRWGCKKRNVLEKVLYHDEL 438


>gb|AFX59322.1| endo-1,4-beta-mannanase [Populus trichocarpa]
          Length = 471

 Score =  710 bits (1833), Expect = 0.0
 Identities = 323/411 (78%), Positives = 371/411 (90%)
 Frame = +2

Query: 41   EMADMEDNAWAMVKKQGNQFVVNNQPFYVNGFNTYWLMVFAVDQSTRAKVSEVFQQASSV 220
            E+ D+E++ W MV K+GNQFV+N+QPFYVNGFNTYWLMVFA DQSTR KV+EVFQ+ASSV
Sbjct: 61   ELNDVEEDEWLMVAKKGNQFVINDQPFYVNGFNTYWLMVFAADQSTRGKVTEVFQKASSV 120

Query: 221  GLTICRTWAFNDGQWRALQKSPSVYDEDVFKALDFVISEAKKYKIRLILSLTNNWDAYGG 400
            GL++CRTWAFNDGQWRALQKSP VYDEDVFKALDFV+SEA KYKIRLILSL NNWDAYGG
Sbjct: 121  GLSVCRTWAFNDGQWRALQKSPGVYDEDVFKALDFVVSEANKYKIRLILSLANNWDAYGG 180

Query: 401  KPQYVKWGKDAGLNLTSEDEFFSHPTLRRYYKAHVKTVLNRVNTVNNITYKDDPTIFAWE 580
            K QYVKWGK +GLNLTS+D+FFSHPTLR YYKAHVK VLNRVNT+ NITYKDDPTIFAWE
Sbjct: 181  KAQYVKWGKASGLNLTSDDDFFSHPTLRSYYKAHVKAVLNRVNTITNITYKDDPTIFAWE 240

Query: 581  LMNEPRCPSDSSGNTLQEWIEEMAVYVKTIDPNHLVEIGLEGFYGPSTPNRVQFNPNTYA 760
            LMNEPRC SD SG+ LQ WI +MAVYVK++D  HLVEIGLEGFYGPS P+R QFNPN+YA
Sbjct: 241  LMNEPRCTSDPSGDKLQSWITDMAVYVKSMDAKHLVEIGLEGFYGPSAPDRAQFNPNSYA 300

Query: 761  QQIGTDFIRNHNILGVDFASVHIYADSWISQLVDDSHLKFIKSWMEAHIEDAENVLGMPV 940
             Q+GTDFIRNH +LGVDFASVHIYADSWISQ + DSH++F KSWMEAHIEDAE  LGMPV
Sbjct: 301  TQVGTDFIRNHQVLGVDFASVHIYADSWISQTITDSHIQFTKSWMEAHIEDAEKYLGMPV 360

Query: 941  IFGEFGVSTKDPGYNSSYRDKVLSTVYNTLLNSTKKGGSGAGALIWQVFPEGTDYMDDGY 1120
            +F EFGVS+KDPGYNSS+RD +++TVY TLLNSTK+GGSGAG+L+WQ+FP+GTDYMDDGY
Sbjct: 361  VFAEFGVSSKDPGYNSSFRDTLINTVYKTLLNSTKRGGSGAGSLLWQLFPDGTDYMDDGY 420

Query: 1121 AIVLSKSPSTSNIVSLHSKRLAMFNSLCSWRCHWGCKKKHKLEDEFFHDEL 1273
            AIVLSKSPST+NI+SLHS R+A+ NS+CSW+C WGCKK++ LE   +HD+L
Sbjct: 421  AIVLSKSPSTTNIISLHSTRVAIVNSMCSWKCKWGCKKRNPLEAFLYHDDL 471


>ref|XP_002309191.1| predicted protein [Populus trichocarpa] gi|222855167|gb|EEE92714.1|
            predicted protein [Populus trichocarpa]
          Length = 415

 Score =  706 bits (1823), Expect = 0.0
 Identities = 327/417 (78%), Positives = 373/417 (89%), Gaps = 1/417 (0%)
 Frame = +2

Query: 26   VIRDSEMADMEDNAWAMVKKQGNQFVVNNQPFYVNGFNTYWLMVFAVDQSTRAKVSEVFQ 205
            + R  E+ D+ ++ W MV ++GNQFV+N+QPFYVNGFNTYWLMVFA DQSTR KV+EVFQ
Sbjct: 1    MFRAYELNDVGEDEWQMVARKGNQFVINDQPFYVNGFNTYWLMVFAADQSTRGKVTEVFQ 60

Query: 206  QASSVGLTICRTWAFNDGQWRALQKSPSVYDEDVFK-ALDFVISEAKKYKIRLILSLTNN 382
            QASSVGLT+CRTWAFNDGQWRALQKSP VYDEDVFK ALDFV+SEAKKYKIRLILSLTNN
Sbjct: 61   QASSVGLTVCRTWAFNDGQWRALQKSPGVYDEDVFKLALDFVVSEAKKYKIRLILSLTNN 120

Query: 383  WDAYGGKPQYVKWGKDAGLNLTSEDEFFSHPTLRRYYKAHVKTVLNRVNTVNNITYKDDP 562
            WDAYGGK QYVKWGK  GLNLTS+D+FFSHPTLR YYKAH   VLNRVNT+ NITYKDDP
Sbjct: 121  WDAYGGKAQYVKWGKATGLNLTSDDDFFSHPTLRSYYKAHA--VLNRVNTLTNITYKDDP 178

Query: 563  TIFAWELMNEPRCPSDSSGNTLQEWIEEMAVYVKTIDPNHLVEIGLEGFYGPSTPNRVQF 742
            TIFAWELMNEPRC SD SG+ LQ WI +MAVYVK++D  HLVEIGLEGFYGPS P+R QF
Sbjct: 179  TIFAWELMNEPRCTSDPSGDKLQSWITDMAVYVKSMDAKHLVEIGLEGFYGPSAPDRAQF 238

Query: 743  NPNTYAQQIGTDFIRNHNILGVDFASVHIYADSWISQLVDDSHLKFIKSWMEAHIEDAEN 922
            NPN+YA Q+GTDFIRNH +LGVDFASVHIYADSWIS  + DSH++FIKSWMEAHIEDAE 
Sbjct: 239  NPNSYATQVGTDFIRNHQVLGVDFASVHIYADSWISLTISDSHIQFIKSWMEAHIEDAER 298

Query: 923  VLGMPVIFGEFGVSTKDPGYNSSYRDKVLSTVYNTLLNSTKKGGSGAGALIWQVFPEGTD 1102
             LGMPV+F EFGVS+KDPGYN+S+RD +++TVY TLLNSTK+GGSGAG+L+WQ+FP+GTD
Sbjct: 299  YLGMPVVFAEFGVSSKDPGYNTSFRDTMINTVYKTLLNSTKRGGSGAGSLLWQIFPDGTD 358

Query: 1103 YMDDGYAIVLSKSPSTSNIVSLHSKRLAMFNSLCSWRCHWGCKKKHKLEDEFFHDEL 1273
            YMDDGYAIVLSKSPSTSNI+SLHS R+A+FNS+CSW+C WGCKKK+ LE  F+HD+L
Sbjct: 359  YMDDGYAIVLSKSPSTSNIISLHSTRVAIFNSMCSWKCKWGCKKKNPLEAFFYHDDL 415


>emb|CBI18019.3| unnamed protein product [Vitis vinifera]
          Length = 471

 Score =  706 bits (1822), Expect = 0.0
 Identities = 321/411 (78%), Positives = 368/411 (89%)
 Frame = +2

Query: 41   EMADMEDNAWAMVKKQGNQFVVNNQPFYVNGFNTYWLMVFAVDQSTRAKVSEVFQQASSV 220
            EM D  D  W MV+K+GNQFVVN++PFYVNGFNTYWLMVFAVDQSTR KVSEVFQQA+SV
Sbjct: 61   EMGDFGDEPWTMVQKKGNQFVVNDKPFYVNGFNTYWLMVFAVDQSTRGKVSEVFQQAASV 120

Query: 221  GLTICRTWAFNDGQWRALQKSPSVYDEDVFKALDFVISEAKKYKIRLILSLTNNWDAYGG 400
            GLT+CRTWAFNDGQWRALQKSPSVYDE+VFKALDFV+SEA+KYKIRLILSL+NNW+ YGG
Sbjct: 121  GLTVCRTWAFNDGQWRALQKSPSVYDEEVFKALDFVVSEARKYKIRLILSLSNNWEGYGG 180

Query: 401  KPQYVKWGKDAGLNLTSEDEFFSHPTLRRYYKAHVKTVLNRVNTVNNITYKDDPTIFAWE 580
            K QYVKWGK+AGLNLTS+D+FFSHPTLR YYKA+VKTVLNRVNT  NITYK+DPTIFAWE
Sbjct: 181  KAQYVKWGKEAGLNLTSDDDFFSHPTLRSYYKANVKTVLNRVNTFTNITYKEDPTIFAWE 240

Query: 581  LMNEPRCPSDSSGNTLQEWIEEMAVYVKTIDPNHLVEIGLEGFYGPSTPNRVQFNPNTYA 760
            LMNEPRC SD +G+ LQ WI+EM +YVK++DP HL+EIGLEGFYGPSTP++VQFNPNTYA
Sbjct: 241  LMNEPRCTSDPTGDKLQSWIQEMVIYVKSMDPKHLLEIGLEGFYGPSTPDKVQFNPNTYA 300

Query: 761  QQIGTDFIRNHNILGVDFASVHIYADSWISQLVDDSHLKFIKSWMEAHIEDAENVLGMPV 940
            QQ+GTDFIRNH +LGVDFASVHIY DSWISQ + ++HL F KSWM+AHIED+E  LGMPV
Sbjct: 301  QQVGTDFIRNHQVLGVDFASVHIYTDSWISQSITEAHLDFTKSWMQAHIEDSEKYLGMPV 360

Query: 941  IFGEFGVSTKDPGYNSSYRDKVLSTVYNTLLNSTKKGGSGAGALIWQVFPEGTDYMDDGY 1120
            +FGEFGVS+KD GYNSS+RD  +STVY  LLNSTKKGGSGAG+L+WQ+FP+G DYMDDGY
Sbjct: 361  VFGEFGVSSKDNGYNSSFRDTFISTVYKILLNSTKKGGSGAGSLLWQLFPDGADYMDDGY 420

Query: 1121 AIVLSKSPSTSNIVSLHSKRLAMFNSLCSWRCHWGCKKKHKLEDEFFHDEL 1273
            AIVLSKSPSTSN+++L S RL MFNS+C+W C W C KKH L+   FHD+L
Sbjct: 421  AIVLSKSPSTSNVIALQSTRLMMFNSMCAWNCRWSCHKKHPLDAFLFHDDL 471


>ref|XP_002273389.1| PREDICTED: mannan endo-1,4-beta-mannosidase 6-like [Vitis vinifera]
          Length = 410

 Score =  704 bits (1817), Expect = 0.0
 Identities = 320/410 (78%), Positives = 367/410 (89%)
 Frame = +2

Query: 44   MADMEDNAWAMVKKQGNQFVVNNQPFYVNGFNTYWLMVFAVDQSTRAKVSEVFQQASSVG 223
            M D  D  W MV+K+GNQFVVN++PFYVNGFNTYWLMVFAVDQSTR KVSEVFQQA+SVG
Sbjct: 1    MGDFGDEPWTMVQKKGNQFVVNDKPFYVNGFNTYWLMVFAVDQSTRGKVSEVFQQAASVG 60

Query: 224  LTICRTWAFNDGQWRALQKSPSVYDEDVFKALDFVISEAKKYKIRLILSLTNNWDAYGGK 403
            LT+CRTWAFNDGQWRALQKSPSVYDE+VFKALDFV+SEA+KYKIRLILSL+NNW+ YGGK
Sbjct: 61   LTVCRTWAFNDGQWRALQKSPSVYDEEVFKALDFVVSEARKYKIRLILSLSNNWEGYGGK 120

Query: 404  PQYVKWGKDAGLNLTSEDEFFSHPTLRRYYKAHVKTVLNRVNTVNNITYKDDPTIFAWEL 583
             QYVKWGK+AGLNLTS+D+FFSHPTLR YYKA+VKTVLNRVNT  NITYK+DPTIFAWEL
Sbjct: 121  AQYVKWGKEAGLNLTSDDDFFSHPTLRSYYKANVKTVLNRVNTFTNITYKEDPTIFAWEL 180

Query: 584  MNEPRCPSDSSGNTLQEWIEEMAVYVKTIDPNHLVEIGLEGFYGPSTPNRVQFNPNTYAQ 763
            MNEPRC SD +G+ LQ WI+EM +YVK++DP HL+EIGLEGFYGPSTP++VQFNPNTYAQ
Sbjct: 181  MNEPRCTSDPTGDKLQSWIQEMVIYVKSMDPKHLLEIGLEGFYGPSTPDKVQFNPNTYAQ 240

Query: 764  QIGTDFIRNHNILGVDFASVHIYADSWISQLVDDSHLKFIKSWMEAHIEDAENVLGMPVI 943
            Q+GTDFIRNH +LGVDFASVHIY DSWISQ + ++HL F KSWM+AHIED+E  LGMPV+
Sbjct: 241  QVGTDFIRNHQVLGVDFASVHIYTDSWISQSITEAHLDFTKSWMQAHIEDSEKYLGMPVV 300

Query: 944  FGEFGVSTKDPGYNSSYRDKVLSTVYNTLLNSTKKGGSGAGALIWQVFPEGTDYMDDGYA 1123
            FGEFGVS+KD GYNSS+RD  +STVY  LLNSTKKGGSGAG+L+WQ+FP+G DYMDDGYA
Sbjct: 301  FGEFGVSSKDNGYNSSFRDTFISTVYKILLNSTKKGGSGAGSLLWQLFPDGADYMDDGYA 360

Query: 1124 IVLSKSPSTSNIVSLHSKRLAMFNSLCSWRCHWGCKKKHKLEDEFFHDEL 1273
            IVLSKSPSTSN+++L S RL MFNS+C+W C W C KKH L+   FHD+L
Sbjct: 361  IVLSKSPSTSNVIALQSTRLMMFNSMCAWNCRWSCHKKHPLDAFLFHDDL 410