BLASTX nr result
ID: Bupleurum21_contig00025332
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00025332
(1059 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAK54465.1| cold-induced glucosyl transferase [Solanum sogara... 328 2e-87
dbj|BAM28983.1| UDP-glucose crocetin glucosyltransferase [Garden... 322 8e-86
dbj|BAK55736.1| UDP-glucose glucosyltransferase [Gardenia jasmin... 320 5e-85
dbj|BAB88935.1| glucosyltransferase [Nicotiana tabacum] 315 2e-83
dbj|BAG80544.1| UDP-glucose:glucosyltransferase [Lycium barbarum] 311 1e-82
>gb|AAK54465.1| cold-induced glucosyl transferase [Solanum sogarandinum]
Length = 473
Score = 328 bits (840), Expect = 2e-87
Identities = 168/340 (49%), Positives = 232/340 (68%), Gaps = 19/340 (5%)
Frame = +1
Query: 97 HILLITYPAQGHINPSLHLAERLLNTGMQVTFTTSISGRRCMP----TTLPNGLRLNLFS 264
H+LL+T+P QGHINPSL A++L+ G++VTFTTS+ R M +T P GL L FS
Sbjct: 5 HVLLVTFPTQGHINPSLQFAKKLIKMGIEVTFTTSVFAHRRMAKTATSTAPKGLNLAAFS 64
Query: 265 NGYDDGIKNTADSDDFYV-KLRSCGTQNIKETIISSAEQCHPVTCLVYTIFFPWVAEVAR 441
+G+DDG K+ D Y+ ++RS G+Q +++ I+ S+++ PVT LVYT+ PW AEVAR
Sbjct: 65 DGFDDGFKSNVDDSKRYMSEIRSRGSQTLRDIILKSSDEGRPVTSLVYTLLLPWAAEVAR 124
Query: 442 GFHLPAVLFWIQPAAVLDIYYYYFHGYGEEID-KHNDPSWSIKLRGLPLLHKNDLPSFLF 618
H+P+ L WIQPA VLDIYYYYF+GY +E+ NDP+WSI+L LPLL DLPSFL
Sbjct: 125 ELHIPSALLWIQPATVLDIYYYYFNGYEDEMKCSSNDPNWSIQLPRLPLLKSQDLPSFLV 184
Query: 619 PST--QETYKLAISSLREHFDALN--DHPKVLVNSFDDLECDALRAIDKLNMVAIGPVIP 786
S+ + Y A+ + +E D L+ ++PKVLVN+FD LE + L+AI K N++ IGP+IP
Sbjct: 185 SSSSKDDKYSFALPTFKEQLDTLDGEENPKVLVNTFDALELEPLKAIGKYNLIGIGPLIP 244
Query: 787 SDFL-------KENSNDHDQDTSKNYINWLNTKEDSSVIYISFGTMLVLPKQEMEEIASG 945
S FL D Q ++ +Y+ WLNTK SS++YISFG++L L + + EEIA G
Sbjct: 245 SSFLGGKDSLESRFGGDLFQKSNDDYMEWLNTKPKSSIVYISFGSLLNLSRNQKEEIAKG 304
Query: 946 LIKTKRPFLWIMR--KNEYEYENQEQKLECMSKLEEQGMI 1059
LI+ KRPFLW++R +N E E +E+KL CM +LE+QG I
Sbjct: 305 LIEIKRPFLWVIRDQENIKEVEKEEEKLSCMMELEKQGKI 344
>dbj|BAM28983.1| UDP-glucose crocetin glucosyltransferase [Gardenia jasminoides]
Length = 474
Score = 322 bits (826), Expect = 8e-86
Identities = 166/338 (49%), Positives = 224/338 (66%), Gaps = 14/338 (4%)
Frame = +1
Query: 88 RRHHILLITYPAQGHINPSLHLAERLLNTGMQVTFTTSISGRRCMPTT---LPNGLRLNL 258
++ H+LLITYPAQGHINP+L A+RLL G+QVT TS+ M + P GL
Sbjct: 3 QQRHVLLITYPAQGHINPALQFAQRLLRMGIQVTLATSVYALSRMTKSSGSTPKGLTFAT 62
Query: 259 FSNGYDDGIK-NTADSDDFYVKLRSCGTQNIKETIISSAEQCHPVTCLVYTIFFPWVAEV 435
FS+GYDDG + D ++ L G+ ++ I +SA+Q PVTCLVYT+ PW A V
Sbjct: 63 FSDGYDDGFRPKGVDHTEYMSSLAKQGSNTLRNVINTSADQGCPVTCLVYTLLLPWAATV 122
Query: 436 ARGFHLPAVLFWIQPAAVLDIYYYYFHGYGEEI-DKHNDPSWSIKLRGLPLLHKNDLPSF 612
AR H+P+ L WIQP AV+DIYYYYF GY +++ + NDP+WSI+ GLP + DLPSF
Sbjct: 123 ARECHIPSALLWIQPVAVMDIYYYYFRGYEDDVKNNSNDPTWSIQFPGLPSMKAKDLPSF 182
Query: 613 LFPSTQETYKLAISSLREHFDALN--DHPKVLVNSFDDLECDALRAIDKLNMVAIGPVIP 786
+ PS+ Y A+ + ++ + L+ + PKVLVN+FD LE AL+AI+ N++AIGP+ P
Sbjct: 183 ILPSSDNIYSFALPTFKKQLETLDEEERPKVLVNTFDALEPQALKAIESYNLIAIGPLTP 242
Query: 787 SDFL-----KENSNDHDQ-DTSKNYINWLNTKEDSSVIYISFGTMLVLPKQEMEEIASGL 948
S FL E S D SK+Y WLN++ D SV+Y+SFG++L LPKQ+MEEIA GL
Sbjct: 243 SAFLDGKDPSETSFSGDLFQKSKDYKEWLNSRPDGSVVYVSFGSLLTLPKQQMEEIARGL 302
Query: 949 IKTKRPFLWIMR-KNEYEYENQEQKLECMSKLEEQGMI 1059
+K+ RPFLW++R K E E +E +L CM +LEEQGMI
Sbjct: 303 LKSGRPFLWVIRAKENGEEEKEEDRLICMEELEEQGMI 340
>dbj|BAK55736.1| UDP-glucose glucosyltransferase [Gardenia jasminoides]
Length = 474
Score = 320 bits (819), Expect = 5e-85
Identities = 165/338 (48%), Positives = 224/338 (66%), Gaps = 14/338 (4%)
Frame = +1
Query: 88 RRHHILLITYPAQGHINPSLHLAERLLNTGMQVTFTTSI---SGRRCMPTTLPNGLRLNL 258
++ H+LLITYPAQGHINP+L A+RLL G+QVT TS+ S + + P GL
Sbjct: 3 QQRHVLLITYPAQGHINPALQFAQRLLRMGIQVTLATSVYALSRMKKSSGSTPKGLTFAT 62
Query: 259 FSNGYDDGIK-NTADSDDFYVKLRSCGTQNIKETIISSAEQCHPVTCLVYTIFFPWVAEV 435
FS+GYDDG + D ++ L G+ ++ I +SA+Q PVTCLVYT+ PW A V
Sbjct: 63 FSDGYDDGFRPKGVDHTEYMSSLAKQGSNTLRNVINTSADQGCPVTCLVYTLLLPWAATV 122
Query: 436 ARGFHLPAVLFWIQPAAVLDIYYYYFHGYGEEI-DKHNDPSWSIKLRGLPLLHKNDLPSF 612
AR H+P+ L WIQP AV+DIYYYYF GY +++ + NDP+WSI+ GLP + DLPSF
Sbjct: 123 ARECHIPSALLWIQPVAVMDIYYYYFRGYEDDVKNNSNDPTWSIQFPGLPSMKAKDLPSF 182
Query: 613 LFPSTQETYKLAISSLREHFDALN--DHPKVLVNSFDDLECDALRAIDKLNMVAIGPVIP 786
+ PS+ Y A+ + ++ + L+ + PKVLVN+FD LE AL+AI+ N++AIGP+ P
Sbjct: 183 ILPSSDNIYSFALPTFKKQLETLDEEERPKVLVNTFDALEPQALKAIESYNLIAIGPLTP 242
Query: 787 SDFL-----KENSNDHDQ-DTSKNYINWLNTKEDSSVIYISFGTMLVLPKQEMEEIASGL 948
S FL E S D SK+Y WLN++ SV+Y+SFG++L LPKQ+MEEIA GL
Sbjct: 243 SAFLDGKDPSETSFSGDLFQKSKDYKEWLNSRPAGSVVYVSFGSLLTLPKQQMEEIARGL 302
Query: 949 IKTKRPFLWIMR-KNEYEYENQEQKLECMSKLEEQGMI 1059
+K+ RPFLW++R K E E +E +L CM +LEEQGMI
Sbjct: 303 LKSGRPFLWVIRAKENGEEEKEEDRLICMEELEEQGMI 340
>dbj|BAB88935.1| glucosyltransferase [Nicotiana tabacum]
Length = 470
Score = 315 bits (806), Expect = 2e-83
Identities = 166/337 (49%), Positives = 230/337 (68%), Gaps = 16/337 (4%)
Frame = +1
Query: 97 HILLITYPAQGHINPSLHLAERLLNTGMQVTFTTSISGRRCMP----TTLPNGLRLNLFS 264
H+LL+T+PAQGHINP L A+RL+ G++VTF TS+ R M +TL GL FS
Sbjct: 5 HVLLVTFPAQGHINPCLQFAKRLIRMGIEVTFATSVFAHRRMAKTTTSTLSKGLNFAAFS 64
Query: 265 NGYDDGIK-NTADSDDFYVKLRSCGTQNIKETIISSAEQCHPVTCLVYTIFFPWVAEVAR 441
+GYDDG K + DS + +++S G++ +K+ I+ S+++ PVT LVY++ PW A+VAR
Sbjct: 65 DGYDDGFKADEHDSQHYMSEIKSRGSKTLKDIILKSSDEGRPVTSLVYSLLLPWAAKVAR 124
Query: 442 GFHLPAVLFWIQPAAVLDIYYYYFHGYGEEI-DKHNDPSWSIKLRGLPLLHKNDLPSFLF 618
FH+P L WIQPA VLDIYYYYF+GY + I NDP+W I+L LPLL DLPSFL
Sbjct: 125 EFHIPCALLWIQPATVLDIYYYYFNGYEDAIKGSTNDPNWCIQLPRLPLLKSQDLPSFLL 184
Query: 619 PST-QETYKLAISSLREHFDALN--DHPKVLVNSFDDLECDALRAIDKLNMVAIGPVIPS 789
S+ +E Y A+ + +E D L+ ++PKVLVN+FD LE L+AI+K N++ IGP+IPS
Sbjct: 185 SSSNEEKYSFALPTFKEQLDTLDVEENPKVLVNTFDALEPKELKAIEKYNLIGIGPLIPS 244
Query: 790 DFLK-----ENSNDHDQ-DTSKNYINWLNTKEDSSVIYISFGTMLVLPKQEMEEIASGLI 951
FL ++S D S +YI WLN+K +SSV+YISFG++L L K + EEIA GLI
Sbjct: 245 TFLDGKDPLDSSFGGDLFQKSNDYIEWLNSKANSSVVYISFGSLLNLSKNQKEEIAKGLI 304
Query: 952 KTKRPFLWIMRKNEY-EYENQEQKLECMSKLEEQGMI 1059
+ K+PFLW++R E + + +E+KL CM +LE+QG I
Sbjct: 305 EIKKPFLWVIRDQENGKGDEKEEKLSCMMELEKQGKI 341
>dbj|BAG80544.1| UDP-glucose:glucosyltransferase [Lycium barbarum]
Length = 469
Score = 311 bits (798), Expect = 1e-82
Identities = 162/341 (47%), Positives = 229/341 (67%), Gaps = 18/341 (5%)
Frame = +1
Query: 91 RHHILLITYPAQGHINPSLHLAERLLNTGMQVTFTTSISGRRCMP----TTLPNGLRLNL 258
R H+LL+T+PAQGHINPSL A+RL+ G++VTF TS+ R M +T P GL
Sbjct: 3 RPHVLLVTFPAQGHINPSLQFAKRLIKLGIEVTFATSVFAHRRMTKTAASTAPEGLNFVA 62
Query: 259 FSNGYDDGIK-NTADSDDFYVKLRSCGTQNIKETIISSAEQCHPVTCLVYTIFFPWVAEV 435
FS+G+DDG K +T D + ++RS G+Q +++ I+ S++ PVT LVYT+ PW AEV
Sbjct: 63 FSDGFDDGFKLDTDDGKRYMSEIRSRGSQTLRDIILKSSDDGRPVTSLVYTLLLPWAAEV 122
Query: 436 ARGFHLPAVLFWIQPAAVLDIYYYYFHGYGEEI-DKHNDPSWSIKLRGLPLLHKNDLPSF 612
AR H+P L WIQPAAVLDIYYYYF+GY +E+ +DP+W I+L GLPLL DLPSF
Sbjct: 123 AREHHIPCALLWIQPAAVLDIYYYYFNGYEDEMKSSTDDPTWRIQLPGLPLLKSQDLPSF 182
Query: 613 LFPSTQE---TYKLAISSLREHFDALN--DHPKVLVNSFDDLECDALRAIDKLNMVAIGP 777
L S + Y A+ + +E D L+ ++PKVLVN+FD LE +AL+AI+K N++ IGP
Sbjct: 183 LVASNSKLNGKYSSALPTFKEQLDTLDGEENPKVLVNTFDALEPEALKAIEKYNLIGIGP 242
Query: 778 VIPSDFLKENSNDHDQ-------DTSKNYINWLNTKEDSSVIYISFGTMLVLPKQEMEEI 936
++PS F + + D S +Y+ WL+++ SS++YISFG++L L + + EEI
Sbjct: 243 LVPSSFF-DGKDPLDSAFGGDLFQKSNDYMEWLDSQPKSSIVYISFGSLLNLSRNQKEEI 301
Query: 937 ASGLIKTKRPFLWIMRKNEYEYENQEQKLECMSKLEEQGMI 1059
A GLI+ KRPFLW++R + E +E++L CM +LE+QG I
Sbjct: 302 AKGLIEIKRPFLWVIR--DQENVKEEEELSCMMELEKQGKI 340