BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00025304
         (1639 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002520694.1| shikimate dehydrogenase, putative [Ricinus c...   573   e-161
ref|XP_002320370.1| dehydroquinate dehydratase/ shikimate dehydr...   556   e-156
ref|XP_002320371.1| predicted protein [Populus trichocarpa] gi|2...   474   e-131
gb|ABK24375.1| unknown [Picea sitchensis]                             467   e-129
ref|XP_002328402.1| dehydroquinate dehydratase/ shikimate dehydr...   464   e-128

>ref|XP_002520694.1| shikimate dehydrogenase, putative [Ricinus communis]
            gi|223540079|gb|EEF41656.1| shikimate dehydrogenase,
            putative [Ricinus communis]
          Length = 515

 Score =  573 bits (1476), Expect = e-161
 Identities = 291/437 (66%), Positives = 341/437 (78%), Gaps = 2/437 (0%)
 Frame = -3

Query: 1559 SKKRSLQVLRLAVELDVEFVEIELEVASDALISEL--KRSNTKIIVSSYVNGGHPSIEKL 1386
            SKK  LQVL LA++LDVEFVE+E EVASD  ++E    RS+ K+IVSSYVNGG PS EKL
Sbjct: 79   SKKTCLQVLSLALDLDVEFVEMEYEVASDVSMAEYIYNRSSGKLIVSSYVNGGKPSAEKL 138

Query: 1385 GNLIAFMQCTGAXXXXXXXXXXXXXXVAPMLHIITHCQVPLIARAAGDRGLISQLLGPKY 1206
            GNLIA +Q TGA              +AP+  ++ HCQVPLIA A G RGLISQLLGPK+
Sbjct: 139  GNLIANLQSTGADVIKLVINVDYITDLAPVFKMLAHCQVPLIALAVGSRGLISQLLGPKF 198

Query: 1205 GSFLVFGSLGGKSIPGLPFVESIKQIYKLECVNADTKVFGVISNPVGHSKGPLLHNPAFR 1026
            G FLV+GSL  K++PG+P + S++ +YKLE +N+DTKVFG+ISNPVGHSKGP+LHNPAFR
Sbjct: 199  GGFLVYGSLRDKAVPGMPTLFSLRHVYKLEYINSDTKVFGLISNPVGHSKGPILHNPAFR 258

Query: 1025 HLGYNGIYAPLLVDNINEFFRVYSASDYAGFSVGLPHKEDAVYCCDEVHPLAKSIGAVNT 846
            H GYNGIY P+ VD+I EFFR Y+++D+AGFSVG+PHKE AV CCDEVHPLAKSIGAVNT
Sbjct: 259  HTGYNGIYVPMQVDDIKEFFRTYTSTDFAGFSVGIPHKEAAVSCCDEVHPLAKSIGAVNT 318

Query: 845  IVRRPTDGKLVGYNTDCEACITAIEDGLRGRQIANGESSNASPXXXXXXXXXXXXXXXXX 666
            IVRRPTDGKLVGYNTDCEA ITAIED LR R+  N  + + SP                 
Sbjct: 319  IVRRPTDGKLVGYNTDCEASITAIEDALRERRPTNRGALDTSPLAGKTFVLIGAGGAGRA 378

Query: 665  XXXXAKSRGARVVIFNRKFERARALAQAVSGEAFPIESLDTFSPTNGMILANASAIGMEP 486
                A SRGARVV+FNR FERA++LA AVSGEA P ++LD F   N MILANASA+GMEP
Sbjct: 379  LAFGASSRGARVVVFNRNFERAKSLANAVSGEALPYKALDRFQRENDMILANASAVGMEP 438

Query: 485  NIHETPVPKEALRSYELIFDAVYTPRNTRLMQEATEVGAIVVSGVEMFIRQAIGQFKLFT 306
            N H++PV KEAL+SYEL+FDAVYTPRNTRL+QEA EVGA+VVSGVEMFIRQA+GQF+LFT
Sbjct: 439  NTHQSPVSKEALKSYELVFDAVYTPRNTRLLQEAKEVGAVVVSGVEMFIRQALGQFRLFT 498

Query: 305  GGLVPEEFMRRTVLEHF 255
            GGL PE FMR+ VLE F
Sbjct: 499  GGLAPEAFMRKLVLEQF 515


>ref|XP_002320370.1| dehydroquinate dehydratase/ shikimate dehydrogenase [Populus
            trichocarpa] gi|222861143|gb|EEE98685.1| dehydroquinate
            dehydratase/ shikimate dehydrogenase [Populus
            trichocarpa]
          Length = 504

 Score =  556 bits (1434), Expect = e-156
 Identities = 288/436 (66%), Positives = 336/436 (77%), Gaps = 2/436 (0%)
 Frame = -3

Query: 1556 KKRSLQVLRLAVELDVEFVEIELEVASDALISEL--KRSNTKIIVSSYVNGGHPSIEKLG 1383
            K   LQVLRLA +L+VEFVE++ EVAS+  ++E    RSNTK+IVSSYVNG  PS E+LG
Sbjct: 80   KNTCLQVLRLAFDLNVEFVEMDYEVASEDDMAEYVYNRSNTKLIVSSYVNGRKPSAEELG 139

Query: 1382 NLIAFMQCTGAXXXXXXXXXXXXXXVAPMLHIITHCQVPLIARAAGDRGLISQLLGPKYG 1203
             LIA MQ TGA              +AP+  ++THCQ+PLIA A G RGLISQLLGPK+G
Sbjct: 140  YLIACMQSTGADVLKLVLDVEKITDLAPVFTMLTHCQIPLIALAVGSRGLISQLLGPKFG 199

Query: 1202 SFLVFGSLGGKSIPGLPFVESIKQIYKLECVNADTKVFGVISNPVGHSKGPLLHNPAFRH 1023
             FLV+GSL  K++PG+P + S++QIYKLE +NADTKVFG+ISNPVGHSKGP+LHNPAFRH
Sbjct: 200  GFLVYGSLSDKAVPGMPTLLSLRQIYKLEYINADTKVFGLISNPVGHSKGPVLHNPAFRH 259

Query: 1022 LGYNGIYAPLLVDNINEFFRVYSASDYAGFSVGLPHKEDAVYCCDEVHPLAKSIGAVNTI 843
             GYNGIY P+ VD++ EFFR Y++SD+AGFSVG+PHKE AV CCDEVHPLAKSIGAVNTI
Sbjct: 260  TGYNGIYVPMQVDDVKEFFRTYTSSDFAGFSVGIPHKEAAVGCCDEVHPLAKSIGAVNTI 319

Query: 842  VRRPTDGKLVGYNTDCEACITAIEDGLRGRQIANGESSNASPXXXXXXXXXXXXXXXXXX 663
            VRRPTDGKLVGYNTDC+A I+AIED L            ASP                  
Sbjct: 320  VRRPTDGKLVGYNTDCDASISAIEDAL-----------TASPLSGKTFVLIGAGGAGRAL 368

Query: 662  XXXAKSRGARVVIFNRKFERARALAQAVSGEAFPIESLDTFSPTNGMILANASAIGMEPN 483
               AKSRGARV+IFNR +ERARALA+AVSGEA P ESLD F P NGMILANASAIGMEPN
Sbjct: 369  AFGAKSRGARVIIFNRNYERARALAKAVSGEALPYESLDRFRPVNGMILANASAIGMEPN 428

Query: 482  IHETPVPKEALRSYELIFDAVYTPRNTRLMQEATEVGAIVVSGVEMFIRQAIGQFKLFTG 303
              ++PV KE L++ EL+FDAVYTPRNTRL++EA EVGA+VVSGVEMFIRQA+GQF+LFTG
Sbjct: 429  SDQSPVSKEILKACELVFDAVYTPRNTRLLREAKEVGAVVVSGVEMFIRQALGQFRLFTG 488

Query: 302  GLVPEEFMRRTVLEHF 255
            GL PE FMR+ VLE F
Sbjct: 489  GLAPEAFMRKLVLEQF 504


>ref|XP_002320371.1| predicted protein [Populus trichocarpa] gi|222861144|gb|EEE98686.1|
            predicted protein [Populus trichocarpa]
          Length = 337

 Score =  474 bits (1220), Expect = e-131
 Identities = 238/340 (70%), Positives = 274/340 (80%)
 Frame = -3

Query: 1274 QVPLIARAAGDRGLISQLLGPKYGSFLVFGSLGGKSIPGLPFVESIKQIYKLECVNADTK 1095
            Q+PLIA A G RGLISQLLGPK+G FLV+GSL  K++PG+P + S++QIYKLE +NADTK
Sbjct: 1    QIPLIALAVGSRGLISQLLGPKFGGFLVYGSLSDKAVPGMPTLLSLRQIYKLEYINADTK 60

Query: 1094 VFGVISNPVGHSKGPLLHNPAFRHLGYNGIYAPLLVDNINEFFRVYSASDYAGFSVGLPH 915
            VFG+ISNPVGHSKGP+LHNPAFRH GYNGIY P+ VD++ EFFR Y++SD+AGFSVG+PH
Sbjct: 61   VFGLISNPVGHSKGPVLHNPAFRHTGYNGIYVPMQVDDVKEFFRTYTSSDFAGFSVGIPH 120

Query: 914  KEDAVYCCDEVHPLAKSIGAVNTIVRRPTDGKLVGYNTDCEACITAIEDGLRGRQIANGE 735
            KE AV CCDEVHPLAKSIGAVNTIVRRPTDGKLVGYNTDC+A I+AIED L G ++    
Sbjct: 121  KEAAVGCCDEVHPLAKSIGAVNTIVRRPTDGKLVGYNTDCDASISAIEDALTGTKLI--- 177

Query: 734  SSNASPXXXXXXXXXXXXXXXXXXXXXAKSRGARVVIFNRKFERARALAQAVSGEAFPIE 555
                SP                     AKSRGARV+IFNR +ERARALA+AVSGEA P E
Sbjct: 178  ILVPSPLSGKTFVLIGAGGAGRALAFGAKSRGARVIIFNRNYERARALAKAVSGEALPYE 237

Query: 554  SLDTFSPTNGMILANASAIGMEPNIHETPVPKEALRSYELIFDAVYTPRNTRLMQEATEV 375
            SLD F P NGMILANASAIGMEPN  ++PV KE L++ EL+FDAVYTPRNTRL+QEA EV
Sbjct: 238  SLDRFRPVNGMILANASAIGMEPNSDQSPVSKETLKACELVFDAVYTPRNTRLLQEAKEV 297

Query: 374  GAIVVSGVEMFIRQAIGQFKLFTGGLVPEEFMRRTVLEHF 255
             A+VVSGVEMFIRQA+GQF+LFTGGL PE FMR+ VLE F
Sbjct: 298  VAVVVSGVEMFIRQALGQFRLFTGGLAPEAFMRKLVLEQF 337


>gb|ABK24375.1| unknown [Picea sitchensis]
          Length = 514

 Score =  467 bits (1201), Expect = e-129
 Identities = 241/441 (54%), Positives = 314/441 (71%), Gaps = 4/441 (0%)
 Frame = -3

Query: 1565 DCSKKRSLQVLRLAVELDVEFVEIELEVASDAL--ISELKRSNTKIIVSSYVNGGHPSIE 1392
            +  +K+ L  LRLA EL  +F+++EL+VA D +  I + K SN KIIVS++++G  PS E
Sbjct: 75   EVDEKKRLDTLRLAHELGADFIDVELQVAFDFVSRIKDKKSSNCKIIVSNHISGRTPSTE 134

Query: 1391 KLGNLIAFMQCTGAXXXXXXXXXXXXXXVAPMLHIITHCQVPLIARAAGDRGLISQLLGP 1212
             L +L+  MQ TGA              V+ +  +I HCQVP+IA   GDRGLISQLLGP
Sbjct: 135  DLSHLVVNMQSTGADILKIVTTATNITDVSRVFQLIPHCQVPIIACVTGDRGLISQLLGP 194

Query: 1211 KYGSFLVFGSLG-GK-SIPGLPFVESIKQIYKLECVNADTKVFGVISNPVGHSKGPLLHN 1038
            K+G +L++GS+G GK S PG P +  +   YK+  +N+DTKVFGVI+NPVGHSKGP+LHN
Sbjct: 195  KFGGYLIYGSIGSGKDSTPGQPKLADLTGHYKIGRLNSDTKVFGVIANPVGHSKGPILHN 254

Query: 1037 PAFRHLGYNGIYAPLLVDNINEFFRVYSASDYAGFSVGLPHKEDAVYCCDEVHPLAKSIG 858
             AFR  G+NG+Y PLLVDN+ +F +VYSA D+AGFSVG+PHKE AV CCDEV PLA+SIG
Sbjct: 255  QAFRDAGFNGVYVPLLVDNLADFLKVYSAPDFAGFSVGIPHKEAAVTCCDEVQPLAQSIG 314

Query: 857  AVNTIVRRPTDGKLVGYNTDCEACITAIEDGLRGRQIANGESSNASPXXXXXXXXXXXXX 678
            AVNTIVRR +DGKL+GYNTDCE  I+AIEDGL+G +  +  S ++SP             
Sbjct: 315  AVNTIVRRQSDGKLIGYNTDCEGAISAIEDGLKG-ETGSINSGSSSPLAGRTFVLVGAGG 373

Query: 677  XXXXXXXXAKSRGARVVIFNRKFERARALAQAVSGEAFPIESLDTFSPTNGMILANASAI 498
                    AK RGA V+I NR +ERA++LA +V GEA  +E L++F P  G +LAN SA+
Sbjct: 374  AGRALAFGAKQRGAHVIIANRNYERAKSLANSVGGEAIAVEQLNSFRPETGAVLANTSAV 433

Query: 497  GMEPNIHETPVPKEALRSYELIFDAVYTPRNTRLMQEATEVGAIVVSGVEMFIRQAIGQF 318
            GM+PN++ TP+ KEAL +Y L+FDAVYTPR TRL++EA E G IVVSG+EMFIRQAIGQ+
Sbjct: 434  GMQPNVNATPLSKEALGAYTLVFDAVYTPRETRLLREAKEAGKIVVSGLEMFIRQAIGQY 493

Query: 317  KLFTGGLVPEEFMRRTVLEHF 255
            +LFTG   P E +R+ V++ F
Sbjct: 494  ELFTGKPAPTELIRKIVMKDF 514


>ref|XP_002328402.1| dehydroquinate dehydratase/ shikimate dehydrogenase [Populus
            trichocarpa] gi|222838117|gb|EEE76482.1| dehydroquinate
            dehydratase/ shikimate dehydrogenase [Populus
            trichocarpa]
          Length = 518

 Score =  464 bits (1193), Expect = e-128
 Identities = 237/435 (54%), Positives = 307/435 (70%), Gaps = 3/435 (0%)
 Frame = -3

Query: 1550 RSLQVLRLAVELDVEFVEIELEVASDALISELKRSNT---KIIVSSYVNGGHPSIEKLGN 1380
            R L+ LRLA +L  +++++EL+VAS+ LI ELK  +    K+IVSSY+NG  PS E L +
Sbjct: 86   RRLEALRLANDLGADYIDLELKVASE-LIWELKNKHQNGGKVIVSSYLNGATPSKENLSH 144

Query: 1379 LIAFMQCTGAXXXXXXXXXXXXXXVAPMLHIITHCQVPLIARAAGDRGLISQLLGPKYGS 1200
            L+A MQ T A              +  + H+++HC+VP +A + G+RGLISQLL PK+G 
Sbjct: 145  LVATMQATEADIIKVVSNADDITEMERIFHLLSHCEVPAVAYSVGERGLISQLLCPKFGG 204

Query: 1199 FLVFGSLGGKSIPGLPFVESIKQIYKLECVNADTKVFGVISNPVGHSKGPLLHNPAFRHL 1020
             LV+GS+ G SIPGLP ++S+++ YK++C+N+DTKVFG++S PVGHSKGPLLHNP  RH+
Sbjct: 205  ALVYGSMEGNSIPGLPTLDSLREAYKVDCINSDTKVFGLVSKPVGHSKGPLLHNPTLRHV 264

Query: 1019 GYNGIYAPLLVDNINEFFRVYSASDYAGFSVGLPHKEDAVYCCDEVHPLAKSIGAVNTIV 840
             +NGIY P+ VD++ +FF VY++ D+AG+SVG P+KE  V  CDEVHPLAK IGAVNTI+
Sbjct: 265  NFNGIYVPMFVDDLKKFFDVYASPDFAGYSVGFPYKEAVVQFCDEVHPLAKCIGAVNTII 324

Query: 839  RRPTDGKLVGYNTDCEACITAIEDGLRGRQIANGESSNASPXXXXXXXXXXXXXXXXXXX 660
            RRP DGKL+GYNTDCE  ITAIED LR ++  NG S N SP                   
Sbjct: 325  RRPCDGKLIGYNTDCEGSITAIEDALRDQKYVNGRSLN-SPLAGKQFVVVGAGGAGRAIA 383

Query: 659  XXAKSRGARVVIFNRKFERARALAQAVSGEAFPIESLDTFSPTNGMILANASAIGMEPNI 480
              AKSRGAR++IF+   ERA++LA+AVSGEA   ESL  F P NG ILANA+ IGM P+ 
Sbjct: 384  VGAKSRGARLIIFDIDLERAKSLARAVSGEAQHFESLAHFQPENGAILANATPIGMHPST 443

Query: 479  HETPVPKEALRSYELIFDAVYTPRNTRLMQEATEVGAIVVSGVEMFIRQAIGQFKLFTGG 300
               P  +E L +Y+L+FDAVYTPR TRL+++A   GAI VSGVEMF+RQAIGQF LFTG 
Sbjct: 444  DRIPAAEETLGNYQLVFDAVYTPRKTRLLKDADAAGAITVSGVEMFLRQAIGQFNLFTGR 503

Query: 299  LVPEEFMRRTVLEHF 255
              P++FMR  VL  F
Sbjct: 504  EAPKDFMREIVLAKF 518