BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00025247
         (1226 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002264202.1| PREDICTED: uncharacterized protein LOC100265...   253   6e-92
ref|XP_002526980.1| S-adenosylmethionine-dependent methyltransfe...   224   3e-83
ref|XP_003595865.1| hypothetical protein MTR_2g062700 [Medicago ...   237   2e-82
ref|XP_003532895.1| PREDICTED: uncharacterized protein LOC100785...   226   2e-81
ref|XP_002297904.1| predicted protein [Populus trichocarpa] gi|2...   231   2e-80

>ref|XP_002264202.1| PREDICTED: uncharacterized protein LOC100265576 [Vitis vinifera]
           gi|296087558|emb|CBI34147.3| unnamed protein product
           [Vitis vinifera]
          Length = 328

 Score =  253 bits (647), Expect(2) = 6e-92
 Identities = 120/185 (64%), Positives = 149/185 (80%), Gaps = 3/185 (1%)
 Frame = -3

Query: 678 LAGGTAAHLMLDLWPSLQLEGWEIDEILIDKARVYLGLSDLEKHNEDGGILRVRAGDALD 499
           L GGTAAHL+LDLWPSL+LEGWEIDEILI K+R YLGLSDLEKHN+ GGIL V  GDAL 
Sbjct: 138 LGGGTAAHLLLDLWPSLKLEGWEIDEILIHKSREYLGLSDLEKHNQAGGILHVHVGDALS 197

Query: 498 ASTTAPGGYAGIVVDLFSNGKVLPEMHEVETWLKMKDKLMPKGRIMVNCGGSD-NEASVS 322
                PGG+AGI++DLFS GKVLP++ +  TWL +KDKLM  GR+MVNCGG+D N A +S
Sbjct: 198 PLVNVPGGFAGIIIDLFSEGKVLPQLQQYGTWLILKDKLMANGRLMVNCGGADYNSAQIS 257

Query: 321 D-DSSWEQNNTIKAICQAFPGEVSWRKLI-KEGENYLALTGPLPDLSTWSACLPDRLSSS 148
             +  W +N+T+KA+C+AFPG++SW+K+  +EGENYLALTGP PDL +WSA +P  LSS+
Sbjct: 258 SINGVWVKNSTLKALCRAFPGQLSWKKMSEREGENYLALTGPFPDLKSWSATVPGWLSSN 317

Query: 147 VMQWK 133
           V QW+
Sbjct: 318 VEQWR 322



 Score =  112 bits (279), Expect(2) = 6e-92
 Identities = 53/67 (79%), Positives = 61/67 (91%)
 Frame = -1

Query: 914 QMVTAVKSRYNDILIVDTADSRMLLLDSTYNVHSIFNKGEKWTGSYWDEFASLPAVVTDG 735
           Q++TA+KS YNDILI+DT  SR+LLLDST+NVHSIFNK EK TGSYWDEFASLPA+V +G
Sbjct: 72  QVLTAIKSEYNDILILDTPKSRLLLLDSTHNVHSIFNKEEKRTGSYWDEFASLPAIVPEG 131

Query: 734 PIAIFGL 714
           PIAIFGL
Sbjct: 132 PIAIFGL 138


>ref|XP_002526980.1| S-adenosylmethionine-dependent methyltransferase, putative [Ricinus
           communis] gi|223533671|gb|EEF35407.1|
           S-adenosylmethionine-dependent methyltransferase,
           putative [Ricinus communis]
          Length = 353

 Score =  224 bits (571), Expect(2) = 3e-83
 Identities = 109/191 (57%), Positives = 138/191 (72%), Gaps = 9/191 (4%)
 Frame = -3

Query: 678 LAGGTAAHLMLDLWPSLQLEGWEIDEILIDKARVYLGLSDLEKHNEDGGILRVRAGDALD 499
           L  GTAAHLMLDLWPSL+LEGWEID ILIDKAR Y GLSDLEK    GGIL V   DAL 
Sbjct: 157 LGAGTAAHLMLDLWPSLELEGWEIDRILIDKARQYFGLSDLEKPTTAGGILNVHIDDALS 216

Query: 498 ASTTAPGGYAGIVVDLFSNGKVLPEMHEVETWLKMKDKLMPKGRIMVNCGGSDNEASVSD 319
            +    G YAGIV+DLF  GKVLP++ EV TWLK+  +LM  GRIM NCGG + ++  +D
Sbjct: 217 PTENDSGKYAGIVIDLFCEGKVLPQLQEVATWLKLNGRLMANGRIMANCGGINEKSDNAD 276

Query: 318 --------DSSWEQNNTIKAICQAFPGEVSWRKLIK-EGENYLALTGPLPDLSTWSACLP 166
                   D +W +N+TIK + +AFPG+V+W+++ + +G NYLALTGPLPDL+ WS+ +P
Sbjct: 277 GTIHPESIDGTWIENSTIKTLSEAFPGQVNWKRMPETQGANYLALTGPLPDLTLWSSKVP 336

Query: 165 DRLSSSVMQWK 133
           D LS +V +W+
Sbjct: 337 DPLSENVRRWR 347



 Score =  112 bits (280), Expect(2) = 3e-83
 Identities = 50/67 (74%), Positives = 62/67 (92%)
 Frame = -1

Query: 914 QMVTAVKSRYNDILIVDTADSRMLLLDSTYNVHSIFNKGEKWTGSYWDEFASLPAVVTDG 735
           +++TA++S YNDI+IVDTA +RMLLLDST+NVHSI NKG+KWTGSYWDEFASLPA++ +G
Sbjct: 91  KVLTAMRSSYNDIVIVDTAKARMLLLDSTHNVHSILNKGQKWTGSYWDEFASLPAIIPEG 150

Query: 734 PIAIFGL 714
           PIAI GL
Sbjct: 151 PIAILGL 157


>ref|XP_003595865.1| hypothetical protein MTR_2g062700 [Medicago truncatula]
           gi|124360180|gb|ABN08193.1| hypothetical protein
           MtrDRAFT_AC155884g5v2 [Medicago truncatula]
           gi|355484913|gb|AES66116.1| hypothetical protein
           MTR_2g062700 [Medicago truncatula]
          Length = 330

 Score =  237 bits (605), Expect(2) = 2e-82
 Identities = 117/189 (61%), Positives = 143/189 (75%), Gaps = 7/189 (3%)
 Frame = -3

Query: 678 LAGGTAAHLMLDLWPSLQLEGWEIDEILIDKARVYLGLSDLEKHNEDGGILRVRAGDALD 499
           L GGTAAHLML+LWP+LQLEGWEID+ILIDKAR Y GLSDLEK NEDGGIL V  GDA  
Sbjct: 134 LGGGTAAHLMLELWPALQLEGWEIDDILIDKARDYFGLSDLEKTNEDGGILNVHIGDAFI 193

Query: 498 ASTTAPGGYAGIVVDLFSNGKVLPEMHEVETWLKMKDKLMPKGRIMVNCGGSD------N 337
            S      YAGIVVDLFS+GKVLP++ EV TWL++ D+LM  GR MVNCGG D      +
Sbjct: 194 PSDDLHRRYAGIVVDLFSDGKVLPQLQEVSTWLELHDRLMANGRFMVNCGGIDGVDGSTD 253

Query: 336 EASVSDDSSWEQNNTIKAICQAFPGEVSWRKLIKE-GENYLALTGPLPDLSTWSACLPDR 160
              +S+D +W  N  IKA+ +AFPG++SW+++ KE G+N++ALTGPLPDL +WSA +P  
Sbjct: 254 PKKLSNDEAWLLNAAIKALLKAFPGQLSWKRMPKETGQNFMALTGPLPDLKSWSANVPSP 313

Query: 159 LSSSVMQWK 133
           LS+ VM WK
Sbjct: 314 LSTGVMDWK 322



 Score = 96.3 bits (238), Expect(2) = 2e-82
 Identities = 45/67 (67%), Positives = 55/67 (82%)
 Frame = -1

Query: 914 QMVTAVKSRYNDILIVDTADSRMLLLDSTYNVHSIFNKGEKWTGSYWDEFASLPAVVTDG 735
           Q++T+VK+ YN I+IVDT  S MLLLDS++NVHSI  K +KWT SYWDEF+SLPA+V  G
Sbjct: 68  QILTSVKTDYNHIMIVDTPKSTMLLLDSSHNVHSILYKDKKWTNSYWDEFSSLPAIVPKG 127

Query: 734 PIAIFGL 714
           PIAI GL
Sbjct: 128 PIAILGL 134


>ref|XP_003532895.1| PREDICTED: uncharacterized protein LOC100785365 [Glycine max]
          Length = 341

 Score =  226 bits (577), Expect(2) = 2e-81
 Identities = 110/192 (57%), Positives = 137/192 (71%), Gaps = 10/192 (5%)
 Frame = -3

Query: 678 LAGGTAAHLMLDLWPSLQLEGWEIDEILIDKARVYLGLSDLEKHNEDGGILRVRAGDALD 499
           L GGTAAHLMLDLWPSLQL+GWEID+ILIDKAR Y GLSDLEK  ++GG+L V  GD   
Sbjct: 148 LGGGTAAHLMLDLWPSLQLDGWEIDQILIDKARDYFGLSDLEKTTDNGGVLNVHIGDVFI 207

Query: 498 ASTTAPGGYAGIVVDLFSNGKVLPEMHEVETWLKMKDKLMPKGRIMVNCGG--------- 346
            S      YAGI+VDLFS+GKVLP++ EV TWL++ ++LM  GR MVNCGG         
Sbjct: 208 TSEDFHQRYAGIIVDLFSDGKVLPQLQEVSTWLELHERLMANGRFMVNCGGVGGGPSAVD 267

Query: 345 SDNEASVSDDSSWEQNNTIKAICQAFPGEVSWRKLIKE-GENYLALTGPLPDLSTWSACL 169
              +   S D SW  N  ++A+ +AFPG+VSW+++ KE GEN++ALTGPLPDL +WSA +
Sbjct: 268 GSTDQETSSDESWLLNPALQALSKAFPGQVSWKRMPKENGENFMALTGPLPDLDSWSASV 327

Query: 168 PDRLSSSVMQWK 133
           P  LS SV  W+
Sbjct: 328 PSPLSKSVKNWR 339



 Score =  103 bits (258), Expect(2) = 2e-81
 Identities = 49/67 (73%), Positives = 57/67 (85%)
 Frame = -1

Query: 914 QMVTAVKSRYNDILIVDTADSRMLLLDSTYNVHSIFNKGEKWTGSYWDEFASLPAVVTDG 735
           Q++TA+K+ YNDILIVDT  SRMLLLDS+Y+VHSI  K +KWTGSYWDEFASLP +V  G
Sbjct: 82  QVLTALKTDYNDILIVDTPKSRMLLLDSSYSVHSILYKEQKWTGSYWDEFASLPVIVPKG 141

Query: 734 PIAIFGL 714
           PIAI GL
Sbjct: 142 PIAILGL 148


>ref|XP_002297904.1| predicted protein [Populus trichocarpa] gi|222845162|gb|EEE82709.1|
           predicted protein [Populus trichocarpa]
          Length = 357

 Score =  231 bits (588), Expect(2) = 2e-80
 Identities = 110/190 (57%), Positives = 146/190 (76%), Gaps = 8/190 (4%)
 Frame = -3

Query: 678 LAGGTAAHLMLDLWPSLQLEGWEIDEILIDKARVYLGLSDLEKHNEDGGILRVRAGDALD 499
           L GGTAAHLMLD+WPSLQLEGWEIDEILI+KAR Y GLSDLEK  + GG+L V  GDAL 
Sbjct: 162 LGGGTAAHLMLDVWPSLQLEGWEIDEILINKARDYFGLSDLEKQTQAGGMLHVVVGDALC 221

Query: 498 ASTTAPGGYAGIVVDLFSNGKVLPEMHEVETWLKMKDKLMPKGRIMVNCGGSDNEASVSD 319
           +       YAGIV+DLF  GKVLP++ EV TWL++K +L+P GR+MVNCGG +   ++++
Sbjct: 222 SLEDDGRKYAGIVIDLFYGGKVLPQLQEVATWLELKGRLIPNGRLMVNCGGIEESDAINE 281

Query: 318 -------DSSWEQNNTIKAICQAFPGEVSWRKLIK-EGENYLALTGPLPDLSTWSACLPD 163
                  D++W +N TIK +C+AFPG++SW+++ + EG NYLALTGPLPDL++WSA +P 
Sbjct: 282 RISTKSVDNAWVENPTIKVLCEAFPGQLSWKRVPESEGANYLALTGPLPDLTSWSAMVPV 341

Query: 162 RLSSSVMQWK 133
            LS++V +W+
Sbjct: 342 HLSAAVSKWR 351



 Score = 96.3 bits (238), Expect(2) = 2e-80
 Identities = 49/78 (62%), Positives = 60/78 (76%), Gaps = 11/78 (14%)
 Frame = -1

Query: 914 QMVTAVKSRYNDILIVDTADSRMLLLDSTYNVHSIFNK-GEKWTGSYW----------DE 768
           +++TAVKS+YNDILIVDT  +RMLLLDST+NVHS+  K G+KWT SYW          DE
Sbjct: 85  KVLTAVKSQYNDILIVDTPKTRMLLLDSTHNVHSLLYKDGQKWTRSYWVCMLSPFVMQDE 144

Query: 767 FASLPAVVTDGPIAIFGL 714
           FASLPA++  GP+AIFGL
Sbjct: 145 FASLPAIIPQGPVAIFGL 162