BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00025230
         (1169 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003519098.1| PREDICTED: nitrate transporter 1.2-like [Gly...   370   e-100
ref|XP_003613584.1| Nitrate transporter NTL1 [Medicago truncatul...   365   2e-98
ref|XP_003517767.1| PREDICTED: nitrate transporter 1.2-like [Gly...   360   4e-97
ref|XP_003551989.1| PREDICTED: nitrate transporter 1.2-like [Gly...   350   4e-94
ref|XP_002311879.1| predicted protein [Populus trichocarpa] gi|2...   349   7e-94

>ref|XP_003519098.1| PREDICTED: nitrate transporter 1.2-like [Glycine max]
          Length = 580

 Score =  370 bits (949), Expect = e-100
 Identities = 187/334 (55%), Positives = 228/334 (68%)
 Frame = +1

Query: 166  MNTWEGYVDWKNRPAIRGRHGGMLASSFVVLVEVLENLAFLANASNLVRYLKDHMHMLPS 345
            ++TWEGYVDW+N+PA+RGRHGGMLA+SFV++ E+LENLAFLANASNLV YL+ +MHM PS
Sbjct: 8    VSTWEGYVDWRNKPALRGRHGGMLAASFVLVAEILENLAFLANASNLVLYLRQYMHMSPS 67

Query: 346  DSSNSVTNXXXXXXXXXXXXXXXXXXXXTSFYIYXXXXXXXXXXXXXXXVQAWSASLKPP 525
             S+N+VTN                    T++ +Y               +QA   SLKPP
Sbjct: 68   KSANNVTNFMGTAFLLALLGGFLSDAFFTTYRVYLISAVIEFLGLIVLTIQARDPSLKPP 127

Query: 526  KCEVHDTACDEVDGAKAAMLFLGLYIVALGVGGIKGSLPTHGAEQFDEHTPEGRKQRSTF 705
            KC++ DT C EV+G+KAAMLF+GLY+VALGVGGIKGSLP HG EQFDE TP GRKQRSTF
Sbjct: 128  KCDL-DTPCQEVNGSKAAMLFIGLYLVALGVGGIKGSLPAHGGEQFDETTPSGRKQRSTF 186

Query: 706  FNYFVFCLAFGGLIAVTLVVWIEDNKGWEWGFGISTLTILLSIPVFLSGSPFYRNKIPNG 885
            FNYFVFCL+ G LIAVT VVWIEDNKGW+WGF IST++I +SIPVFL+GSP Y+NKIP+G
Sbjct: 187  FNYFVFCLSCGALIAVTFVVWIEDNKGWQWGFAISTISIFVSIPVFLAGSPTYKNKIPSG 246

Query: 886  SPLTTIFKVLIASALNTCVAAGMXXXXXXXXXXXXXXXXXXXXXDGGEGTDGSSKQTDEP 1065
            SPLTTI KVLIA+ LN+C                           G   +   + +    
Sbjct: 247  SPLTTILKVLIAALLNSCT------YKNTSSAVVNMTSSPSNPHSGRTESQQETVKASTT 300

Query: 1066 NEGPSESLTFLNRAVTRKPAYKSLQFTINQVEDV 1167
             E P+ +L FLN+AVT KP Y SL+ T+ QVEDV
Sbjct: 301  TETPTSNLKFLNKAVTNKPRYSSLECTVQQVEDV 334


>ref|XP_003613584.1| Nitrate transporter NTL1 [Medicago truncatula]
            gi|355514919|gb|AES96542.1| Nitrate transporter NTL1
            [Medicago truncatula]
          Length = 582

 Score =  365 bits (936), Expect = 2e-98
 Identities = 187/334 (55%), Positives = 225/334 (67%)
 Frame = +1

Query: 166  MNTWEGYVDWKNRPAIRGRHGGMLASSFVVLVEVLENLAFLANASNLVRYLKDHMHMLPS 345
            M  WEGYVDW+N+PA+RGRHGGM+A+SFV++VE+LENLA+LANASNLV YLK++MHM PS
Sbjct: 10   MTRWEGYVDWRNKPALRGRHGGMVAASFVLVVEILENLAYLANASNLVLYLKEYMHMSPS 69

Query: 346  DSSNSVTNXXXXXXXXXXXXXXXXXXXXTSFYIYXXXXXXXXXXXXXXXVQAWSASLKPP 525
             S+N+VTN                    T++++Y               +QA   SLKP 
Sbjct: 70   KSANNVTNFMGTSFLLALLGGFLSDAFLTTYHVYLISAVIELIGLIILTIQAHVPSLKPT 129

Query: 526  KCEVHDTACDEVDGAKAAMLFLGLYIVALGVGGIKGSLPTHGAEQFDEHTPEGRKQRSTF 705
            KC    T C+EV+G KAAMLF GLY+VALGVGGIKGSLP HG EQFDE TP GRKQRSTF
Sbjct: 130  KCN-ESTPCEEVNGGKAAMLFAGLYLVALGVGGIKGSLPVHGGEQFDEATPNGRKQRSTF 188

Query: 706  FNYFVFCLAFGGLIAVTLVVWIEDNKGWEWGFGISTLTILLSIPVFLSGSPFYRNKIPNG 885
            FNYFVFCL+ G LIAVT VVW+EDNKGWEWGF IST+TI +SIPVFL+GS  YRNK+P+G
Sbjct: 189  FNYFVFCLSCGALIAVTFVVWVEDNKGWEWGFAISTITIFVSIPVFLAGSTTYRNKVPSG 248

Query: 886  SPLTTIFKVLIASALNTCVAAGMXXXXXXXXXXXXXXXXXXXXXDGGEGTDGSSKQTDEP 1065
            SPLTTI KVLIA+ LN+C                             E     + +   P
Sbjct: 249  SPLTTILKVLIAATLNSCCT----NKNSCSAVVNMSSSPSNPSTQAKEQELHQTLKPTTP 304

Query: 1066 NEGPSESLTFLNRAVTRKPAYKSLQFTINQVEDV 1167
            ++ PSESL FLN+A+T KP + SLQ TI Q+EDV
Sbjct: 305  SQIPSESLKFLNKAITNKPIHSSLQCTIQQLEDV 338


>ref|XP_003517767.1| PREDICTED: nitrate transporter 1.2-like [Glycine max]
          Length = 772

 Score =  360 bits (924), Expect = 4e-97
 Identities = 185/334 (55%), Positives = 224/334 (67%)
 Frame = +1

Query: 166  MNTWEGYVDWKNRPAIRGRHGGMLASSFVVLVEVLENLAFLANASNLVRYLKDHMHMLPS 345
            ++TWEGYVDW+N+PA+RGRHGGMLA+SFV++ E+LENLAFLANASNLV YL+ +MHM PS
Sbjct: 201  VSTWEGYVDWRNKPALRGRHGGMLAASFVLVAEILENLAFLANASNLVLYLRHYMHMSPS 260

Query: 346  DSSNSVTNXXXXXXXXXXXXXXXXXXXXTSFYIYXXXXXXXXXXXXXXXVQAWSASLKPP 525
             S+N+VTN                    TS+ +Y               +QA   SLKPP
Sbjct: 261  KSANNVTNFMGTAFILALLGGFLSDAFFTSYRVYLISAVIEFLGLIVLTIQARDPSLKPP 320

Query: 526  KCEVHDTACDEVDGAKAAMLFLGLYIVALGVGGIKGSLPTHGAEQFDEHTPEGRKQRSTF 705
            KC++ DT C EV+ +KAAMLF+GLY+VALGVGGIKGSLP HG EQFDE TP GRKQRSTF
Sbjct: 321  KCDL-DTPCQEVNDSKAAMLFIGLYLVALGVGGIKGSLPAHGGEQFDETTPSGRKQRSTF 379

Query: 706  FNYFVFCLAFGGLIAVTLVVWIEDNKGWEWGFGISTLTILLSIPVFLSGSPFYRNKIPNG 885
            FNYFVFCL+ G LIAVT VVWIEDNKGW+WGF IST++I +SIPVFL+GS  Y+NKIP+G
Sbjct: 380  FNYFVFCLSCGALIAVTFVVWIEDNKGWQWGFAISTISIFVSIPVFLAGSATYKNKIPSG 439

Query: 886  SPLTTIFKVLIASALNTCVAAGMXXXXXXXXXXXXXXXXXXXXXDGGEGTDGSSKQTDEP 1065
            SPLTTI KVL+A+ LN C                           G   +   + +    
Sbjct: 440  SPLTTILKVLVAALLNICT------YKNTSSAVVNMASSPSNPHSGRMESKLETAKASTI 493

Query: 1066 NEGPSESLTFLNRAVTRKPAYKSLQFTINQVEDV 1167
             E P+  L FLN+AVT KP Y SL+ T+ QVEDV
Sbjct: 494  AETPTSHLKFLNKAVTNKPRYSSLECTVQQVEDV 527


>ref|XP_003551989.1| PREDICTED: nitrate transporter 1.2-like [Glycine max]
          Length = 585

 Score =  350 bits (898), Expect = 4e-94
 Identities = 181/331 (54%), Positives = 225/331 (67%)
 Frame = +1

Query: 175  WEGYVDWKNRPAIRGRHGGMLASSFVVLVEVLENLAFLANASNLVRYLKDHMHMLPSDSS 354
            WEGYV+W+N+PA+ G +GGMLA+SFV++VE+LENLAFLANASNLV YL+ +MHM PS S+
Sbjct: 9    WEGYVNWRNKPALSGCNGGMLAASFVLVVEILENLAFLANASNLVLYLRQYMHMSPSKSA 68

Query: 355  NSVTNXXXXXXXXXXXXXXXXXXXXTSFYIYXXXXXXXXXXXXXXXVQAWSASLKPPKCE 534
            N+VTN                    T++ IY               VQA   SLKPP C+
Sbjct: 69   NNVTNFMGTAFLLALLGGFLSDAFFTTYQIYLISAVIEFLGLIVLTVQARVPSLKPPACD 128

Query: 535  VHDTACDEVDGAKAAMLFLGLYIVALGVGGIKGSLPTHGAEQFDEHTPEGRKQRSTFFNY 714
               T C+EV G KAAMLF GLY+VALGVGGIKGSLP+HGAEQFD++TP GRK+RSTFFNY
Sbjct: 129  A-STPCNEVSGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDNTPSGRKKRSTFFNY 187

Query: 715  FVFCLAFGGLIAVTLVVWIEDNKGWEWGFGISTLTILLSIPVFLSGSPFYRNKIPNGSPL 894
            FVFCL+FG LIAVT VVW+EDNKGWEWGFGIST+TI +SIPVFL+GS  YR+KIP+ SPL
Sbjct: 188  FVFCLSFGALIAVTFVVWVEDNKGWEWGFGISTITIFVSIPVFLAGSTTYRSKIPSRSPL 247

Query: 895  TTIFKVLIASALNTCVAAGMXXXXXXXXXXXXXXXXXXXXXDGGEGTDGSSKQTDEPNEG 1074
            TTI KVL+A++LN+C  +                        G E ++ ++K   EP   
Sbjct: 248  TTILKVLVAASLNSCFNSRNSSSAVVNMTSSPSNLNSGRKQVGKEASNIANK---EPEAP 304

Query: 1075 PSESLTFLNRAVTRKPAYKSLQFTINQVEDV 1167
             + +L FLN+AV   P Y S++ T+ QVEDV
Sbjct: 305  ITNTLKFLNKAVENNPIYSSIKCTVEQVEDV 335


>ref|XP_002311879.1| predicted protein [Populus trichocarpa] gi|222851699|gb|EEE89246.1|
            predicted protein [Populus trichocarpa]
          Length = 575

 Score =  349 bits (896), Expect = 7e-94
 Identities = 180/333 (54%), Positives = 224/333 (67%), Gaps = 2/333 (0%)
 Frame = +1

Query: 175  WEGYVDWKNRPAIRGRHGGMLASSFVVLVEVLENLAFLANASNLVRYLKDHMHMLPSDSS 354
            WEGYVDW+NRPA+RG HGGMLA+S+V+ VE+LENLA+LANASNLV YL ++MH+ PS S+
Sbjct: 10   WEGYVDWRNRPALRGLHGGMLAASYVLGVEILENLAYLANASNLVIYLSEYMHLSPSKSA 69

Query: 355  NSVTNXXXXXXXXXXXXXXXXXXXXTSFYIYXXXXXXXXXXXXXXXVQAWSASLKPPKCE 534
            N+VTN                    T+++++               VQA S+SLKPP C 
Sbjct: 70   NNVTNFMGTSFLLALLGGFLADTFFTTYHVFLISAAIEFLGLVILTVQARSSSLKPPACN 129

Query: 535  VHDTA--CDEVDGAKAAMLFLGLYIVALGVGGIKGSLPTHGAEQFDEHTPEGRKQRSTFF 708
              +    C ++DGAKAAMLF GLY+VALGVGGIKGSLP HGAEQFDE TP+GRKQRSTFF
Sbjct: 130  PSNPTIPCQQIDGAKAAMLFTGLYLVALGVGGIKGSLPAHGAEQFDESTPQGRKQRSTFF 189

Query: 709  NYFVFCLAFGGLIAVTLVVWIEDNKGWEWGFGISTLTILLSIPVFLSGSPFYRNKIPNGS 888
            NYFVFCL+ G LIAVT VVW+EDNKGW WGFGIST+ ILLSIP+FL+GS  YRNKIP+GS
Sbjct: 190  NYFVFCLSCGALIAVTFVVWVEDNKGWVWGFGISTIAILLSIPIFLAGSAAYRNKIPSGS 249

Query: 889  PLTTIFKVLIASALNTCVAAGMXXXXXXXXXXXXXXXXXXXXXDGGEGTDGSSKQTDEPN 1068
            PL TI KVL+A+ +NTC++                           +  D S      P 
Sbjct: 250  PLATISKVLVAAGVNTCMSRS-----SGNAIASLTTSPSNPNPTSKDAIDNSIAVM--PA 302

Query: 1069 EGPSESLTFLNRAVTRKPAYKSLQFTINQVEDV 1167
            E P++SL +LN+AV  KP + +L+ T+ QVE+V
Sbjct: 303  ETPTKSLDWLNKAVVNKPIHSALECTVQQVEEV 335