BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00025134
         (1086 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003531241.1| PREDICTED: pheophorbide a oxygenase, chlorop...   195   e-119
ref|XP_003629726.1| Pheophorbide a oxygenase [Medicago truncatul...   188   e-116
ref|XP_003629721.1| Pheophorbide a oxygenase [Medicago truncatul...   187   e-115
ref|XP_003629724.1| Pheophorbide a oxygenase [Medicago truncatul...   180   e-111
ref|XP_002304001.1| predicted protein [Populus trichocarpa] gi|2...   178   e-107

>ref|XP_003531241.1| PREDICTED: pheophorbide a oxygenase, chloroplastic-like [Glycine max]
          Length = 531

 Score =  195 bits (496), Expect(3) = e-119
 Identities = 86/130 (66%), Positives = 106/130 (81%)
 Frame = -1

Query: 1083 IPQAPRDGPPIHTSQKACVSVYPSVVQNGMVWFWPSTDPKNKDILAKRKPPYIPELDDPS 904
            IPQAP DGPP+HTS+KACV+ YPS VQN ++WFWP+TDP+ KDI+ +++PPYIPELDDPS
Sbjct: 165  IPQAPPDGPPVHTSKKACVAAYPSTVQNDILWFWPNTDPQYKDIITRKRPPYIPELDDPS 224

Query: 903  YTKSMGSRDIPYGYEILTENFMDPAHVPYAHYKIMGVRQRKNSEKADREGGKPINIRIKK 724
            YTK MG+RDI YGYE+L EN MDPAHVPYAHY +M  R  K   K DREGG+P+ + I+ 
Sbjct: 225  YTKLMGNRDISYGYEVLIENLMDPAHVPYAHYGLM--RTPKPKVKVDREGGRPLELSIET 282

Query: 723  QDINGYSAEQ 694
             DING++A Q
Sbjct: 283  LDINGFTANQ 292



 Score =  175 bits (443), Expect(3) = e-119
 Identities = 73/113 (64%), Positives = 93/113 (82%)
 Frame = -3

Query: 340 GPSNWHKACFVPTKADTLVVAFRRWLNKHSGGQIDWGTKFGGALPPTPPREQLLDRYWSH 161
           GP NWHKACFVPTK+D LV+ +R WL K++GGQ+DW  K+ GALPPTPPREQL+DRYWSH
Sbjct: 392 GPKNWHKACFVPTKSDALVIGYRNWLKKYAGGQVDWRGKYSGALPPTPPREQLMDRYWSH 451

Query: 160 VVNCRSCSAAYKGLNAVEVALQIIAIALVGFVAVAKQGMLSALMRNFLVAMAI 2
           VVNC+SC++AYK LN +EV LQII++A +G VA+ K G +S   RN +VA+A+
Sbjct: 452 VVNCKSCNSAYKSLNVIEVMLQIISVASIGIVAIMKHGTMSVAKRNSMVALAV 504



 Score =  105 bits (263), Expect(3) = e-119
 Identities = 46/56 (82%), Positives = 51/56 (91%)
 Frame = -2

Query: 629 LIFITVPVSPGHSRLIWSFPRNFGIWIDRIVPRWIFHLGQNLILDSDLYLLHAEER 462
           LIF+ VPVSPG SRLIW FPRNFG+WID+IVPRWIFH+GQN ILDSDLYLLH EE+
Sbjct: 331 LIFVCVPVSPGKSRLIWCFPRNFGVWIDKIVPRWIFHVGQNRILDSDLYLLHVEEQ 386


>ref|XP_003629726.1| Pheophorbide a oxygenase [Medicago truncatula]
            gi|355523748|gb|AET04202.1| Pheophorbide a oxygenase
            [Medicago truncatula]
          Length = 529

 Score =  188 bits (478), Expect(3) = e-116
 Identities = 81/131 (61%), Positives = 107/131 (81%)
 Frame = -1

Query: 1086 FIPQAPRDGPPIHTSQKACVSVYPSVVQNGMVWFWPSTDPKNKDILAKRKPPYIPELDDP 907
            FIPQAP +GPPIHTS+KACV+ YPS VQN ++WFWP++DP+ KDI+ ++ PP+IPE+DDP
Sbjct: 162  FIPQAPPEGPPIHTSKKACVAAYPSTVQNDILWFWPNSDPQYKDIITRKTPPFIPEIDDP 221

Query: 906  SYTKSMGSRDIPYGYEILTENFMDPAHVPYAHYKIMGVRQRKNSEKADREGGKPINIRIK 727
            S+T  MG+RDI YGYE+L EN MDP+HVPYAHY +M   Q K   KADREGG+P+ + I+
Sbjct: 222  SFTSLMGNRDIAYGYEVLIENLMDPSHVPYAHYGLMKTPQPK--VKADREGGRPLELSIE 279

Query: 726  KQDINGYSAEQ 694
            + D+NG++A Q
Sbjct: 280  ELDVNGFTANQ 290



 Score =  172 bits (435), Expect(3) = e-116
 Identities = 74/113 (65%), Positives = 93/113 (82%)
 Frame = -3

Query: 340 GPSNWHKACFVPTKADTLVVAFRRWLNKHSGGQIDWGTKFGGALPPTPPREQLLDRYWSH 161
           G +NWHKACFVPTKAD LV+ FR+WL K++GG+++W  K+ GALPPTPPREQLLDRYWSH
Sbjct: 390 GQANWHKACFVPTKADALVIGFRKWLKKYAGGEVEWRGKYNGALPPTPPREQLLDRYWSH 449

Query: 160 VVNCRSCSAAYKGLNAVEVALQIIAIALVGFVAVAKQGMLSALMRNFLVAMAI 2
            VNC+SC+ AYK LN VEV LQII++A +G VA  KQG++SA  RN +V +A+
Sbjct: 450 TVNCKSCNFAYKSLNVVEVMLQIISVASIGIVATMKQGVVSATTRNSMVLLAV 502



 Score =  108 bits (271), Expect(3) = e-116
 Identities = 47/56 (83%), Positives = 53/56 (94%)
 Frame = -2

Query: 629 LIFITVPVSPGHSRLIWSFPRNFGIWIDRIVPRWIFHLGQNLILDSDLYLLHAEER 462
           LIFI +PVSPG+SRLIW FPRNFG+WID+IVPRWIFH+GQNLILDSDLYLLH EE+
Sbjct: 329 LIFICIPVSPGNSRLIWCFPRNFGLWIDKIVPRWIFHVGQNLILDSDLYLLHVEEK 384


>ref|XP_003629721.1| Pheophorbide a oxygenase [Medicago truncatula]
            gi|355523743|gb|AET04197.1| Pheophorbide a oxygenase
            [Medicago truncatula]
          Length = 532

 Score =  187 bits (476), Expect(3) = e-115
 Identities = 80/131 (61%), Positives = 104/131 (79%)
 Frame = -1

Query: 1086 FIPQAPRDGPPIHTSQKACVSVYPSVVQNGMVWFWPSTDPKNKDILAKRKPPYIPELDDP 907
            FIPQAP +GPPIHTS+KACV+ YPS VQN ++WFWP+TDP+ KDI+ ++ PP+IPE+DDP
Sbjct: 138  FIPQAPPEGPPIHTSKKACVAAYPSTVQNDILWFWPNTDPQYKDIITRKTPPFIPEIDDP 197

Query: 906  SYTKSMGSRDIPYGYEILTENFMDPAHVPYAHYKIMGVRQRKNSEKADREGGKPINIRIK 727
            S+T  MG+RDIPYGYE+L EN MDP+HVPYAHY   G+        ADREGG P++  ++
Sbjct: 198  SFTSFMGNRDIPYGYEVLVENLMDPSHVPYAHY---GLTPDPKGNNADREGGTPLDFSVE 254

Query: 726  KQDINGYSAEQ 694
            + DING++A Q
Sbjct: 255  ELDINGFTANQ 265



 Score =  174 bits (440), Expect(3) = e-115
 Identities = 76/113 (67%), Positives = 92/113 (81%)
 Frame = -3

Query: 340 GPSNWHKACFVPTKADTLVVAFRRWLNKHSGGQIDWGTKFGGALPPTPPREQLLDRYWSH 161
           G  NWHKACFVPTKAD LV+ FR+WL K++G Q+DW  K+ GALPPTPPREQL+DRYWSH
Sbjct: 365 GQGNWHKACFVPTKADALVIGFRKWLKKYAGDQVDWRGKYSGALPPTPPREQLMDRYWSH 424

Query: 160 VVNCRSCSAAYKGLNAVEVALQIIAIALVGFVAVAKQGMLSALMRNFLVAMAI 2
            VNC+SC+ AYK LN VEV LQII++A +GFVA  KQG++SA  RN +V +AI
Sbjct: 425 TVNCKSCNFAYKSLNVVEVMLQIISVASIGFVATMKQGIVSAATRNSIVVLAI 477



 Score =  103 bits (257), Expect(3) = e-115
 Identities = 44/56 (78%), Positives = 50/56 (89%)
 Frame = -2

Query: 629 LIFITVPVSPGHSRLIWSFPRNFGIWIDRIVPRWIFHLGQNLILDSDLYLLHAEER 462
           +IFI +PVSPG SRLIW FPRNFG+W D+IVPRWI H+GQNLILDSDLYLLH EE+
Sbjct: 304 MIFICIPVSPGKSRLIWCFPRNFGVWADKIVPRWILHMGQNLILDSDLYLLHVEEQ 359


>ref|XP_003629724.1| Pheophorbide a oxygenase [Medicago truncatula]
            gi|355523746|gb|AET04200.1| Pheophorbide a oxygenase
            [Medicago truncatula]
          Length = 546

 Score =  180 bits (456), Expect(3) = e-111
 Identities = 82/150 (54%), Positives = 107/150 (71%), Gaps = 19/150 (12%)
 Frame = -1

Query: 1086 FIPQAPRDGPPIHTSQKACVSVYPSVVQNGMVWFWPSTDPKNKDILAKRKPPYIPELDDP 907
            FIPQAP +GPPIHTS+KACV+ YPS VQN ++WFWP+TDP+ KDI+ ++ PP+IPE+DDP
Sbjct: 138  FIPQAPPEGPPIHTSKKACVAAYPSTVQNDILWFWPNTDPQYKDIITRKTPPFIPEIDDP 197

Query: 906  SYTKSMGSRDIPYG-------------------YEILTENFMDPAHVPYAHYKIMGVRQR 784
            S+T  MG+RDIPYG                   YE+L EN MDP+HVPYAHY +M   Q 
Sbjct: 198  SFTSFMGNRDIPYGYDFNFENITLCIPLTVTYMYEVLVENLMDPSHVPYAHYGLMKTPQP 257

Query: 783  KNSEKADREGGKPINIRIKKQDINGYSAEQ 694
            K   KADREGG+P+ + I++ D+NG++  Q
Sbjct: 258  K--VKADREGGRPLELSIEELDVNGFTENQ 285



 Score =  166 bits (419), Expect(3) = e-111
 Identities = 71/113 (62%), Positives = 91/113 (80%)
 Frame = -3

Query: 340 GPSNWHKACFVPTKADTLVVAFRRWLNKHSGGQIDWGTKFGGALPPTPPREQLLDRYWSH 161
           G +NWHKACFVPTKAD LV+ FR+WL K++G Q++W  K+ GALPPTP REQL+DRYWSH
Sbjct: 385 GQANWHKACFVPTKADALVIGFRKWLKKYAGDQVEWRGKYNGALPPTPSREQLMDRYWSH 444

Query: 160 VVNCRSCSAAYKGLNAVEVALQIIAIALVGFVAVAKQGMLSALMRNFLVAMAI 2
           VVNC+SC+ AYK L+ VEV LQII++A +G VA  KQG++S   RN +V +A+
Sbjct: 445 VVNCKSCNLAYKSLSVVEVMLQIISVASIGIVATMKQGLVSTTTRNSMVVLAV 497



 Score =  107 bits (266), Expect(3) = e-111
 Identities = 45/56 (80%), Positives = 52/56 (92%)
 Frame = -2

Query: 629 LIFITVPVSPGHSRLIWSFPRNFGIWIDRIVPRWIFHLGQNLILDSDLYLLHAEER 462
           LIF  +PVSPG+SRLIW FPRNFG+WID+IVPRWIFH+GQNLI+DSDLYLLH EE+
Sbjct: 324 LIFFCIPVSPGNSRLIWCFPRNFGLWIDKIVPRWIFHIGQNLIIDSDLYLLHVEEK 379


>ref|XP_002304001.1| predicted protein [Populus trichocarpa] gi|222841433|gb|EEE78980.1|
            predicted protein [Populus trichocarpa]
          Length = 475

 Score =  178 bits (451), Expect(3) = e-107
 Identities = 76/134 (56%), Positives = 103/134 (76%), Gaps = 2/134 (1%)
 Frame = -1

Query: 1086 FIPQAPRDGPPIHTSQKACVSVYPSVVQNGMVWFWPSTDPKNKDILAKRKPPYIPELDDP 907
            FIPQAP  GPP+HTS KACV+VYPS V + +VWFWP+TDP+ +DI+ K++PP+IPELDDP
Sbjct: 113  FIPQAPPAGPPVHTSMKACVAVYPSTVHHDIVWFWPNTDPQYRDIITKKQPPFIPELDDP 172

Query: 906  SYTKSMGSRDIPYGYEILTENFMDPAHVPYAHYKIMGVRQRKN--SEKADREGGKPINIR 733
            S+TK MG+RD PYGY++L EN +DPAH+PYAHY ++   + +      +DREGG PI++ 
Sbjct: 173  SFTKHMGNRDFPYGYDVLIENLLDPAHLPYAHYGLVNAPKPQGILMHYSDREGGGPIDLS 232

Query: 732  IKKQDINGYSAEQE 691
            +KK D  G+   Q+
Sbjct: 233  VKKLDSQGFFENQD 246



 Score =  160 bits (405), Expect(3) = e-107
 Identities = 72/113 (63%), Positives = 87/113 (76%)
 Frame = -3

Query: 340 GPSNWHKACFVPTKADTLVVAFRRWLNKHSGGQIDWGTKFGGALPPTPPREQLLDRYWSH 161
           GP+NW KACFVPTK+D L+V +RRW NK+SGGQ+DW  K+ GALPPT PREQL DRYWSH
Sbjct: 342 GPANWQKACFVPTKSDALLVGYRRWFNKYSGGQVDWKGKYCGALPPTSPREQLFDRYWSH 401

Query: 160 VVNCRSCSAAYKGLNAVEVALQIIAIALVGFVAVAKQGMLSALMRNFLVAMAI 2
           VVNCRSC+AAYKGLNA+EV LQ+ ++A +G  A  KQ        N +V MA+
Sbjct: 402 VVNCRSCNAAYKGLNALEVILQVASLAFIGVAAATKQN------ANTMVVMAV 448



 Score = 99.4 bits (246), Expect(3) = e-107
 Identities = 41/55 (74%), Positives = 48/55 (87%)
 Frame = -2

Query: 626 IFITVPVSPGHSRLIWSFPRNFGIWIDRIVPRWIFHLGQNLILDSDLYLLHAEER 462
           +FI +PVSPG SRLIW FPRNFG+WID++VPRWIFHL  NL+ DSDLYLLH +ER
Sbjct: 282 VFICIPVSPGRSRLIWIFPRNFGLWIDKVVPRWIFHLKYNLVFDSDLYLLHMQER 336