BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00025129
         (619 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P49173.1|NIP1_NICAL RecName: Full=Probable aquaporin NIP-type...   265   6e-69
ref|XP_003544255.1| PREDICTED: LOW QUALITY PROTEIN: probable aqu...   239   2e-61
ref|XP_003518381.1| PREDICTED: probable aquaporin NIP-type-like ...   238   8e-61
ref|XP_003633704.1| PREDICTED: probable aquaporin NIP-type-like ...   237   1e-60
emb|CBI33542.3| unnamed protein product [Vitis vinifera]              237   1e-60

>sp|P49173.1|NIP1_NICAL RecName: Full=Probable aquaporin NIP-type; AltName:
           Full=Pollen-specific membrane integral protein
           gi|665948|gb|AAA62235.1| putative membrane integral
           protein [Nicotiana alata]
          Length = 270

 Score =  265 bits (676), Expect = 6e-69
 Identities = 129/191 (67%), Positives = 153/191 (80%)
 Frame = -3

Query: 575 NKLYGMVTFPGVALSWGLIVTIMIYTVGHVSGAHFNPAVTITQAIFGRFPWQDVPYYIIS 396
           NK+YG VTFPG+ ++WGLIV +M+YTVG++SGAHFNPAVTIT +IFGRFPW+ VP YII+
Sbjct: 66  NKIYGSVTFPGICVTWGLIVMVMVYTVGYISGAHFNPAVTITFSIFGRFPWKQVPLYIIA 125

Query: 395 QLIGSILASITLATLVDVTPEAYFATVPSGSNAQSLGTEIIATFLLVFVIFGAVTDTRAV 216
           QL+GSILAS TLA L DVTP+AYF TVP GSN QSL  EII +FLL+FVI G  TD RA+
Sbjct: 126 QLMGSILASGTLALLFDVTPQAYFGTVPVGSNGQSLAIEIIISFLLMFVISGVATDDRAI 185

Query: 215 MT*INEAVGLTVTLDVFVAGPISGASMNPARSIGPAVVRHVYSGLWVYVXXXXXXXXXXG 36
                 AVG+T+TL+VFVAGPISGASMNPARSIGPA+V+HVY+GLWVYV           
Sbjct: 186 GQVAGIAVGMTITLNVFVAGPISGASMNPARSIGPAIVKHVYTGLWVYVVGPIIGTLAGA 245

Query: 35  FVYNFLRFTDQ 3
           FVYN +R TD+
Sbjct: 246 FVYNLIRSTDK 256


>ref|XP_003544255.1| PREDICTED: LOW QUALITY PROTEIN: probable aquaporin NIP-type-like
           [Glycine max]
          Length = 273

 Score =  239 bits (611), Expect = 2e-61
 Identities = 117/187 (62%), Positives = 143/187 (76%)
 Frame = -3

Query: 575 NKLYGMVTFPGVALSWGLIVTIMIYTVGHVSGAHFNPAVTITQAIFGRFPWQDVPYYIIS 396
           NK+YG VTFPGV ++WGLIV +MIY++ H+SGAHFNPAVTIT AIF RF ++ VP YI +
Sbjct: 67  NKIYGSVTFPGVCVTWGLIVMVMIYSLRHISGAHFNPAVTITLAIFRRFSYKQVPLYIFA 126

Query: 395 QLIGSILASITLATLVDVTPEAYFATVPSGSNAQSLGTEIIATFLLVFVIFGAVTDTRAV 216
           QL+GSILAS TLA ++DVTP+AYF TVP GSN QSL  E+I TFLL+FVI    TD +AV
Sbjct: 127 QLLGSILASGTLALMLDVTPKAYFGTVPVGSNGQSLVAEVIITFLLMFVISAVSTDDKAV 186

Query: 215 MT*INEAVGLTVTLDVFVAGPISGASMNPARSIGPAVVRHVYSGLWVYVXXXXXXXXXXG 36
                 AVG+T+ L+VF+AGP+SGASMNPARSIGPA+++HVY GLW+YV           
Sbjct: 187 GDFAGVAVGMTIMLNVFIAGPVSGASMNPARSIGPALIKHVYQGLWIYVVGPIVGSIAGA 246

Query: 35  FVYNFLR 15
             YNFLR
Sbjct: 247 LAYNFLR 253


>ref|XP_003518381.1| PREDICTED: probable aquaporin NIP-type-like [Glycine max]
          Length = 261

 Score =  238 bits (606), Expect = 8e-61
 Identities = 119/191 (62%), Positives = 144/191 (75%)
 Frame = -3

Query: 575 NKLYGMVTFPGVALSWGLIVTIMIYTVGHVSGAHFNPAVTITQAIFGRFPWQDVPYYIIS 396
           NK+YG VTFPGV ++WGLIV +MIY++  +SGAHFNPAVTIT AIF RF +++VP YI +
Sbjct: 67  NKIYGSVTFPGVCVTWGLIVMVMIYSLRRISGAHFNPAVTITLAIFRRFSYKEVPLYIFA 126

Query: 395 QLIGSILASITLATLVDVTPEAYFATVPSGSNAQSLGTEIIATFLLVFVIFGAVTDTRAV 216
           QL+GSILAS TLA ++DVTP+AYF TVP GSN QSL  EII TFLL+FVI    TD RAV
Sbjct: 127 QLLGSILASGTLALMLDVTPKAYFGTVPVGSNGQSLVAEIIITFLLMFVISAVSTDDRAV 186

Query: 215 MT*INEAVGLTVTLDVFVAGPISGASMNPARSIGPAVVRHVYSGLWVYVXXXXXXXXXXG 36
                 AVG+T+ L+VF+AGP+SGASMNPARSIGPA+++HVY GLWVYV           
Sbjct: 187 GDFAGVAVGMTIMLNVFIAGPVSGASMNPARSIGPALIKHVYKGLWVYVVGPVVGSIAGA 246

Query: 35  FVYNFLRFTDQ 3
             Y FLR  D+
Sbjct: 247 LAYYFLRSIDK 257


>ref|XP_003633704.1| PREDICTED: probable aquaporin NIP-type-like [Vitis vinifera]
          Length = 281

 Score =  237 bits (605), Expect = 1e-60
 Identities = 120/191 (62%), Positives = 144/191 (75%)
 Frame = -3

Query: 575 NKLYGMVTFPGVALSWGLIVTIMIYTVGHVSGAHFNPAVTITQAIFGRFPWQDVPYYIIS 396
           N +YG VTFPGVA +WGLIV +MIY +GH+SGAHFNPAVTIT AI  RFP+  VP YII 
Sbjct: 67  NGIYGSVTFPGVAATWGLIVLVMIYALGHISGAHFNPAVTITFAILRRFPYWQVPLYIIG 126

Query: 395 QLIGSILASITLATLVDVTPEAYFATVPSGSNAQSLGTEIIATFLLVFVIFGAVTDTRAV 216
           QL+GSILAS TL+ + ++  EAYF TVP+GS+ QSL  EII TFLL+FVI G  TD+RA 
Sbjct: 127 QLMGSILASGTLSFMFNIDREAYFGTVPAGSHGQSLVLEIIITFLLMFVISGVATDSRAT 186

Query: 215 MT*INEAVGLTVTLDVFVAGPISGASMNPARSIGPAVVRHVYSGLWVYVXXXXXXXXXXG 36
                 AVG+T+ L+VFVAGP+SGASMNPARSIGPA+V+HVY GLWVYV          G
Sbjct: 187 GELAGIAVGMTIMLNVFVAGPVSGASMNPARSIGPALVKHVYKGLWVYVIGPIIGAIAGG 246

Query: 35  FVYNFLRFTDQ 3
             YN +RFT++
Sbjct: 247 LTYNLIRFTEK 257


>emb|CBI33542.3| unnamed protein product [Vitis vinifera]
          Length = 466

 Score =  237 bits (605), Expect = 1e-60
 Identities = 120/191 (62%), Positives = 144/191 (75%)
 Frame = -3

Query: 575 NKLYGMVTFPGVALSWGLIVTIMIYTVGHVSGAHFNPAVTITQAIFGRFPWQDVPYYIIS 396
           N +YG VTFPGVA +WGLIV +MIY +GH+SGAHFNPAVTIT AI  RFP+  VP YII 
Sbjct: 67  NGIYGSVTFPGVAATWGLIVLVMIYALGHISGAHFNPAVTITFAILRRFPYWQVPLYIIG 126

Query: 395 QLIGSILASITLATLVDVTPEAYFATVPSGSNAQSLGTEIIATFLLVFVIFGAVTDTRAV 216
           QL+GSILAS TL+ + ++  EAYF TVP+GS+ QSL  EII TFLL+FVI G  TD+RA 
Sbjct: 127 QLMGSILASGTLSFMFNIDREAYFGTVPAGSHGQSLVLEIIITFLLMFVISGVATDSRAT 186

Query: 215 MT*INEAVGLTVTLDVFVAGPISGASMNPARSIGPAVVRHVYSGLWVYVXXXXXXXXXXG 36
                 AVG+T+ L+VFVAGP+SGASMNPARSIGPA+V+HVY GLWVYV          G
Sbjct: 187 GELAGIAVGMTIMLNVFVAGPVSGASMNPARSIGPALVKHVYKGLWVYVIGPIIGAIAGG 246

Query: 35  FVYNFLRFTDQ 3
             YN +RFT++
Sbjct: 247 LTYNLIRFTEK 257



 Score =  110 bits (276), Expect = 2e-22
 Identities = 61/163 (37%), Positives = 88/163 (53%)
 Frame = -3

Query: 575 NKLYGMVTFPGVALSWGLIVTIMIYTVGHVSGAHFNPAVTITQAIFGRFPWQDVPYYIIS 396
           NK+YG VT  G+A++WGL + +++Y++GHVSGAHFNP++TI   + G  P+  VP YI +
Sbjct: 302 NKIYGQVTLLGIAMTWGLTIMVIVYSIGHVSGAHFNPSITIAFFMVGHLPYPQVPLYITA 361

Query: 395 QLIGSILASITLATLVDVTPEAYFATVPSGSNAQSLGTEIIATFLLVFVIFGAVTDTRAV 216
           QLIGS+LA   +AT                                          +RA 
Sbjct: 362 QLIGSLLAICAVATY-----------------------------------------SRAS 380

Query: 215 MT*INEAVGLTVTLDVFVAGPISGASMNPARSIGPAVVRHVYS 87
                 A+G+T+ L       +SGAS+NPARSIGPA+V+H+Y+
Sbjct: 381 GGFAGLAIGMTILL-------VSGASLNPARSIGPAMVKHIYT 416



 Score = 57.0 bits (136), Expect = 3e-06
 Identities = 47/169 (27%), Positives = 83/169 (49%), Gaps = 18/169 (10%)
 Frame = -3

Query: 545 GVALSWGLIVTIMIYTVGHVSGAHFNPAVTITQAIFGRFPWQDVPYYIISQLIGSILASI 366
           G+A+  G+ + + ++  G VSGA  NPA +I  A+  +  ++ +  Y+I  +IG+I   +
Sbjct: 191 GIAV--GMTIMLNVFVAGPVSGASMNPARSIGPALV-KHVYKGLWVYVIGPIIGAIAGGL 247

Query: 365 TLATLVDVTPE---------AYFATVPSGSNAQSLGTEIIATFLLVFV---------IFG 240
           T   L+  T +         +   T+      + L  E+I T++L+F+         I+G
Sbjct: 248 TY-NLIRFTEKPLSELTKTSSLLRTISKSVPRKQLLVEMIGTYILIFMGCGSMVVNKIYG 306

Query: 239 AVTDTRAVMT*INEAVGLTVTLDVFVAGPISGASMNPARSIGPAVVRHV 93
            VT     MT      GLT+ + V+  G +SGA  NP+ +I   +V H+
Sbjct: 307 QVTLLGIAMT-----WGLTIMVIVYSIGHVSGAHFNPSITIAFFMVGHL 350