BLASTX nr result
ID: Bupleurum21_contig00025019
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00025019
(1485 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002524221.1| Serine/threonine-protein kinase Nek8, putati... 637 e-180
ref|XP_003538429.1| PREDICTED: probable E3 ubiquitin-protein lig... 622 e-176
ref|XP_004150638.1| PREDICTED: E3 ubiquitin-protein ligase HERC2... 618 e-174
ref|XP_002282470.1| PREDICTED: X-linked retinitis pigmentosa GTP... 614 e-173
ref|XP_003552762.1| PREDICTED: probable E3 ubiquitin-protein lig... 613 e-173
>ref|XP_002524221.1| Serine/threonine-protein kinase Nek8, putative [Ricinus communis]
gi|223536498|gb|EEF38145.1| Serine/threonine-protein
kinase Nek8, putative [Ricinus communis]
Length = 476
Score = 637 bits (1644), Expect = e-180
Identities = 312/452 (69%), Positives = 355/452 (78%), Gaps = 1/452 (0%)
Frame = +2
Query: 131 MNGDGRMSDEAVEMEEEITQKLVYMWGYLPGALPQRXXXXXXXXXXXXXASDGGTSWKDV 310
MNG+G+ E ++MEE + +KLV+MWGYLPGALPQR G SWKDV
Sbjct: 1 MNGEGK---EVMKMEEMVKEKLVFMWGYLPGALPQRSPILSPVVVRAD-----GYSWKDV 52
Query: 311 CGGGCGFAMALSDSGKLITWGSTDDLGQSYVTSGKHGETPEPFPLPGEVSIAKAAAGWAH 490
CGGGCGFAMA+S+SGKLITWGSTDDLGQSYVTSGKHGE PEPFPLP E SI KAAAGWAH
Sbjct: 53 CGGGCGFAMAISESGKLITWGSTDDLGQSYVTSGKHGEIPEPFPLPTEASIMKAAAGWAH 112
Query: 491 CVSATEAGEVYTWGWKECVPSGKVIEDPAMGSRIDKDIVEQQGSHLTEQVSPRSQTSKPS 670
C++AT+ GEVYTWGWKEC+PSGKV DP++ +KD+ E+ LTEQVSPRSQ+S+ +
Sbjct: 113 CIAATDTGEVYTWGWKECIPSGKVFGDPSVAGGTEKDVFERHSPFLTEQVSPRSQSSRST 172
Query: 671 CGTASAG-GEETSKRRRVSSAKQAAESSATPDEPLSALPCLVAISPGVKITTVAAGGRHT 847
G G G+E++KRRR+SSAKQ+AESS++ DE LSALPCLV ++PGV+ITTVAAGGRHT
Sbjct: 173 GGIDGRGSGDESTKRRRISSAKQSAESSSSGDETLSALPCLVTLNPGVRITTVAAGGRHT 232
Query: 848 LALSDXXXXXXXXXXXXXXXXXXSRIRMVSSPHPVPCIDMSVLGKDXXXXXXXXXXXXXX 1027
LALSD SRIRMVSSPHPVPC+D S GKD
Sbjct: 233 LALSDIGQVWGWGYGGEGQLGLGSRIRMVSSPHPVPCVDPSY-GKDRPAPFSRGGMTSEG 291
Query: 1028 XGCLVPGSYVKGIACGGRHSVVITDAGAVLAFGWGLYGQCGQGSTDDELSPTCVSSLLGI 1207
G VPGSYVKGIACGGRHS VITDAGA+L FGWGLYGQCGQGSTDDELSP CVS LLGI
Sbjct: 292 HGFRVPGSYVKGIACGGRHSAVITDAGALLTFGWGLYGQCGQGSTDDELSPICVSPLLGI 351
Query: 1208 RVAGVAAGLWHTVCISDDGDVYSFGGNQFGQLGTGSDQAETLPRLLDAPSFDNINAKVVS 1387
R+AGVAAGLWHT+C+S DGDVY+FGGNQFGQLGTGSDQAET PRLLDAPS ++I+AKVVS
Sbjct: 352 RIAGVAAGLWHTICVSADGDVYAFGGNQFGQLGTGSDQAETTPRLLDAPSLEDIHAKVVS 411
Query: 1388 CGARHSAVVTDNAKVFCWGWNKYGQLGLGDVI 1483
CGARHSA+V ++ KVFCWGWNKYGQLGLGDVI
Sbjct: 412 CGARHSAIVAEDGKVFCWGWNKYGQLGLGDVI 443
>ref|XP_003538429.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC1-like [Glycine
max]
Length = 480
Score = 622 bits (1604), Expect = e-176
Identities = 309/453 (68%), Positives = 347/453 (76%), Gaps = 2/453 (0%)
Frame = +2
Query: 131 MNGDGRMSDEAVEMEEEIT-QKLVYMWGYLPGALPQRXXXXXXXXXXXXXASDGGTSWKD 307
MNG+G EA+++E EI ++LVYMWGYLPGALPQR + G SWKD
Sbjct: 1 MNGEG---SEAMQVEMEIEKERLVYMWGYLPGALPQRTPLLTPVLVRVPPS---GYSWKD 54
Query: 308 VCGGGCGFAMALSDSGKLITWGSTDDLGQSYVTSGKHGETPEPFPLPGEVSIAKAAAGWA 487
VCGGGCGFAMA+S+ GKLITWGSTDDLGQSYVTSGKHGE PEPFPLP EV+I KAAAGWA
Sbjct: 55 VCGGGCGFAMAISEPGKLITWGSTDDLGQSYVTSGKHGEIPEPFPLPTEVTIVKAAAGWA 114
Query: 488 HCVSATEAGEVYTWGWKECVPSGKVIEDPAMGSRIDKDIVEQQGSHLTEQVSPRSQTSKP 667
HCVS T+ GEVYTWGW+ECVPSGKV + G +KD+ +Q S LTEQVSPRSQ SK
Sbjct: 115 HCVSVTDCGEVYTWGWRECVPSGKVFGESLTGVSPEKDVPGRQSSFLTEQVSPRSQGSKS 174
Query: 668 SCGTASA-GGEETSKRRRVSSAKQAAESSATPDEPLSALPCLVAISPGVKITTVAAGGRH 844
+ GTAS GEE+SKRRRVSSAKQ AE+S++ D+ +ALPCLV ++PGV+I +VAAGGRH
Sbjct: 175 TGGTASGTSGEESSKRRRVSSAKQTAETSSSSDDTQTALPCLVTLNPGVRIASVAAGGRH 234
Query: 845 TLALSDXXXXXXXXXXXXXXXXXXSRIRMVSSPHPVPCIDMSVLGKDXXXXXXXXXXXXX 1024
TLALSD SRIRMVSSPH VPCI+ S GKD
Sbjct: 235 TLALSDIGQVWGWGYGGEGQLGLGSRIRMVSSPHLVPCINSSSYGKDISASLARGSMSSD 294
Query: 1025 XXGCLVPGSYVKGIACGGRHSVVITDAGAVLAFGWGLYGQCGQGSTDDELSPTCVSSLLG 1204
VPGSY+KGIACGGRHS VITDAGAVL FGWGLYGQCGQGSTDDELSP CVSSLLG
Sbjct: 295 GQNFRVPGSYIKGIACGGRHSAVITDAGAVLTFGWGLYGQCGQGSTDDELSPNCVSSLLG 354
Query: 1205 IRVAGVAAGLWHTVCISDDGDVYSFGGNQFGQLGTGSDQAETLPRLLDAPSFDNINAKVV 1384
IR+ GVAAGLWHTVC S DGDVY+FGGNQFGQLGTG DQAET+PRLLD PS +N+N K +
Sbjct: 355 IRIEGVAAGLWHTVCTSADGDVYAFGGNQFGQLGTGGDQAETIPRLLDCPSLENVNVKRI 414
Query: 1385 SCGARHSAVVTDNAKVFCWGWNKYGQLGLGDVI 1483
SCGARH+A++TDN KVFCWGWNKYGQLGLGDVI
Sbjct: 415 SCGARHTALITDNGKVFCWGWNKYGQLGLGDVI 447
Score = 59.7 bits (143), Expect = 2e-06
Identities = 29/68 (42%), Positives = 40/68 (58%)
Frame = +2
Query: 1055 VKGIACGGRHSVVITDAGAVLAFGWGLYGQCGQGSTDDELSPTCVSSLLGIRVAGVAAGL 1234
VK I+CG RH+ +ITD G V +GW YGQ G G D P+ V ++ G VA G
Sbjct: 411 VKRISCGARHTALITDNGKVFCWGWNKYGQLGLGDVIDRNIPSEV-TIEGCVAKNVACGW 469
Query: 1235 WHTVCISD 1258
WHT+ +++
Sbjct: 470 WHTLLLAE 477
>ref|XP_004150638.1| PREDICTED: E3 ubiquitin-protein ligase HERC2-like [Cucumis sativus]
gi|449516435|ref|XP_004165252.1| PREDICTED: E3
ubiquitin-protein ligase HERC2-like [Cucumis sativus]
Length = 491
Score = 618 bits (1594), Expect = e-174
Identities = 303/458 (66%), Positives = 352/458 (76%), Gaps = 7/458 (1%)
Frame = +2
Query: 131 MNGDG-RMSDEAVEMEEEITQ-KLVYMWGYLPGALPQRXXXXXXXXXXXXXA-SDGGTSW 301
MNG+G R DE V MEE++ K+V+MWGYLPGALPQR + W
Sbjct: 1 MNGEGSRGEDEIVGMEEDVKDNKIVFMWGYLPGALPQRSPLLSPVVVRLPGKDATDAHGW 60
Query: 302 KDVCGGGCGFAMALSDSGKLITWGSTDDLGQSYVTSGKHGETPEPFPLPGEVSIAKAAAG 481
+DVCGGGCGFA+A+S +GKLITWG+TDDLGQSYVTSGKHGETPEPFPLP E SI KAAAG
Sbjct: 61 RDVCGGGCGFAVAISANGKLITWGATDDLGQSYVTSGKHGETPEPFPLPTEASIVKAAAG 120
Query: 482 WAHCVSATEAGEVYTWGWKECVPSGKVIEDPAMGSRIDKDIVEQQGSHLTEQVSPRSQTS 661
WAH V+ T+ GEVYTWGWKECVPSGK+ D + G+ ++D++E+Q S + +QVSPR Q S
Sbjct: 121 WAHTVAITDRGEVYTWGWKECVPSGKMFTDLSAGAAYERDVLERQSSSVIDQVSPRLQAS 180
Query: 662 KPSCGTAS----AGGEETSKRRRVSSAKQAAESSATPDEPLSALPCLVAISPGVKITTVA 829
+ S +S AGG E++KRRRVSSAK AAESS++ DE LSA+PCLV ++PGV+IT+V+
Sbjct: 181 RSSGVVSSIEARAGGAESTKRRRVSSAKLAAESSSSSDETLSAVPCLVTLNPGVRITSVS 240
Query: 830 AGGRHTLALSDXXXXXXXXXXXXXXXXXXSRIRMVSSPHPVPCIDMSVLGKDXXXXXXXX 1009
AGGRHTLALSD SRIRMVSSPHP+PCI+ S GKD
Sbjct: 241 AGGRHTLALSDMGQVWGWGYGGEGQLGLGSRIRMVSSPHPIPCIESSSFGKDRSAALARG 300
Query: 1010 XXXXXXXGCLVPGSYVKGIACGGRHSVVITDAGAVLAFGWGLYGQCGQGSTDDELSPTCV 1189
G +PGSYVKGIACGGRHS +TDAGA+L FGWGLYGQCGQG TDDELSPTCV
Sbjct: 301 SASLEGQGSRIPGSYVKGIACGGRHSAAVTDAGALLTFGWGLYGQCGQGCTDDELSPTCV 360
Query: 1190 SSLLGIRVAGVAAGLWHTVCISDDGDVYSFGGNQFGQLGTGSDQAETLPRLLDAPSFDNI 1369
SSLLGIR+ VAAGLWHTVCIS DGD+YSFGGNQFGQLGTG+DQAETLPRLLDAPS +N+
Sbjct: 361 SSLLGIRIESVAAGLWHTVCISSDGDIYSFGGNQFGQLGTGADQAETLPRLLDAPSVENV 420
Query: 1370 NAKVVSCGARHSAVVTDNAKVFCWGWNKYGQLGLGDVI 1483
NAKVVSCGARHSA+V+++ KVFCWGWNKYGQLGLGDVI
Sbjct: 421 NAKVVSCGARHSAIVSEDGKVFCWGWNKYGQLGLGDVI 458
>ref|XP_002282470.1| PREDICTED: X-linked retinitis pigmentosa GTPase regulator [Vitis
vinifera]
Length = 484
Score = 614 bits (1584), Expect = e-173
Identities = 306/456 (67%), Positives = 348/456 (76%), Gaps = 5/456 (1%)
Frame = +2
Query: 131 MNGDGRMSDEAVEMEEEITQKLVYMWGYLPGALPQRXXXXXXXXXXXXXASDGGTSWKDV 310
MNG+G+ DEAV ME + +KLV+M GYLPGALPQR + +W+DV
Sbjct: 1 MNGEGKGPDEAVVMEG-VREKLVFMMGYLPGALPQRSPLLSPIAVRSPASIH---AWRDV 56
Query: 311 CGGGCGFAMALSDSGKLITWGSTDDLGQSYVTSGKHGETPEPFPLPGEVSIAKAAAGWAH 490
CGGGCGFAMA+S+SGKLITWGSTDDLGQSYVTSGKHGETPEPFPLP E SI KAAAGWAH
Sbjct: 57 CGGGCGFAMAISESGKLITWGSTDDLGQSYVTSGKHGETPEPFPLPTEASIVKAAAGWAH 116
Query: 491 CVSATEAGEVYTWGWKECVPSGKVIEDPAMGSRIDKDIVEQQGSHLTEQVSPRSQTSKPS 670
C S TE GEVYTWGWKECVPSGKV DP++G ++KD+ E+Q S LTEQVSPR+Q S+ +
Sbjct: 117 CASVTENGEVYTWGWKECVPSGKVFGDPSVGGSLEKDVFERQSSFLTEQVSPRTQGSRST 176
Query: 671 CGTASA-----GGEETSKRRRVSSAKQAAESSATPDEPLSALPCLVAISPGVKITTVAAG 835
G S GGEE++KRRR+ S+KQAAESS+ DE LSALPCLV ++PGV+I VAAG
Sbjct: 177 AGGVSGTDSKGGGEESTKRRRMLSSKQAAESSSG-DETLSALPCLVTLNPGVRIANVAAG 235
Query: 836 GRHTLALSDXXXXXXXXXXXXXXXXXXSRIRMVSSPHPVPCIDMSVLGKDXXXXXXXXXX 1015
GRHTLALSD SRIRMVSSPHPVPC++ S GK+
Sbjct: 236 GRHTLALSDVGQVWGWGYGGEGQLGLGSRIRMVSSPHPVPCVESSSYGKERSSALARGNV 295
Query: 1016 XXXXXGCLVPGSYVKGIACGGRHSVVITDAGAVLAFGWGLYGQCGQGSTDDELSPTCVSS 1195
VPGSYVK IACGGRHS VITDAGA+L FGWGLYGQCGQGSTDDELSPTCVSS
Sbjct: 296 STEGQSLRVPGSYVKWIACGGRHSAVITDAGALLMFGWGLYGQCGQGSTDDELSPTCVSS 355
Query: 1196 LLGIRVAGVAAGLWHTVCISDDGDVYSFGGNQFGQLGTGSDQAETLPRLLDAPSFDNINA 1375
LLGI++ VAAGLWHTVCIS DGDVY+FGGNQFGQLGTG DQ ETLPRLLDAPS +N +A
Sbjct: 356 LLGIQIERVAAGLWHTVCISADGDVYTFGGNQFGQLGTGVDQPETLPRLLDAPSLENAHA 415
Query: 1376 KVVSCGARHSAVVTDNAKVFCWGWNKYGQLGLGDVI 1483
K+VSCGARHSA++T + K+F WGWNKYGQLGLGDV+
Sbjct: 416 KIVSCGARHSAIITADGKMFSWGWNKYGQLGLGDVV 451
>ref|XP_003552762.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC4-like isoform 1
[Glycine max]
Length = 472
Score = 613 bits (1581), Expect = e-173
Identities = 306/452 (67%), Positives = 345/452 (76%), Gaps = 1/452 (0%)
Frame = +2
Query: 131 MNGDGRMSDEAVEMEEEITQKLVYMWGYLPGALPQRXXXXXXXXXXXXXASDGGTSWKDV 310
MNG+G EA+++E+E LVYMWGYLPGALPQR + G WKDV
Sbjct: 1 MNGEG---SEAMQVEKE---SLVYMWGYLPGALPQRTPLLTPLLVRVPPS---GYFWKDV 51
Query: 311 CGGGCGFAMALSDSGKLITWGSTDDLGQSYVTSGKHGETPEPFPLPGEVSIAKAAAGWAH 490
CGGGCGFAMA+S+ GKLITWGSTDDLGQSYVTSGKHGETPEPFPLP EV+I KAAAGWAH
Sbjct: 52 CGGGCGFAMAISEPGKLITWGSTDDLGQSYVTSGKHGETPEPFPLPTEVTIVKAAAGWAH 111
Query: 491 CVSATEAGEVYTWGWKECVPSGKVIEDPAMGSRIDKDIVEQQGSHLTEQVSPRSQTSKPS 670
CVS T+ GEVYTWGW+ECVPSGKV + G +KD+ +Q S LTEQVSPRSQ SK +
Sbjct: 112 CVSVTDCGEVYTWGWRECVPSGKVFGESLTGVSPEKDVPRRQSSFLTEQVSPRSQGSKST 171
Query: 671 CGTASA-GGEETSKRRRVSSAKQAAESSATPDEPLSALPCLVAISPGVKITTVAAGGRHT 847
GTAS GEE+SKRRRVSSAKQ AE+S++ D+ +ALPCLV ++PGV+I +VAAGGRHT
Sbjct: 172 GGTASGTSGEESSKRRRVSSAKQTAETSSSSDDTQTALPCLVTLNPGVRIASVAAGGRHT 231
Query: 848 LALSDXXXXXXXXXXXXXXXXXXSRIRMVSSPHPVPCIDMSVLGKDXXXXXXXXXXXXXX 1027
LALSD SRIRMVSSPH VPCI+ S GKD
Sbjct: 232 LALSDIGQVWGWGYGGEGQLGLGSRIRMVSSPHLVPCINSSSYGKDMARVSISSDGQNFR 291
Query: 1028 XGCLVPGSYVKGIACGGRHSVVITDAGAVLAFGWGLYGQCGQGSTDDELSPTCVSSLLGI 1207
VPGSY+KGIACGGRHS VITDAGAVL FGWGLYGQCGQGSTDDELSP+CVSSLLGI
Sbjct: 292 ----VPGSYIKGIACGGRHSAVITDAGAVLTFGWGLYGQCGQGSTDDELSPSCVSSLLGI 347
Query: 1208 RVAGVAAGLWHTVCISDDGDVYSFGGNQFGQLGTGSDQAETLPRLLDAPSFDNINAKVVS 1387
++ GVAAGLWHTVC S DGDVY+FGGNQFGQLGTG DQAET+PRLLD PS +N+N K +S
Sbjct: 348 QIEGVAAGLWHTVCTSVDGDVYAFGGNQFGQLGTGGDQAETIPRLLDCPSLENVNVKRIS 407
Query: 1388 CGARHSAVVTDNAKVFCWGWNKYGQLGLGDVI 1483
CGARH+A++ DN KVFCWGWNKYGQLGLGDVI
Sbjct: 408 CGARHTALIADNGKVFCWGWNKYGQLGLGDVI 439
Score = 57.8 bits (138), Expect = 7e-06
Identities = 28/68 (41%), Positives = 39/68 (57%)
Frame = +2
Query: 1055 VKGIACGGRHSVVITDAGAVLAFGWGLYGQCGQGSTDDELSPTCVSSLLGIRVAGVAAGL 1234
VK I+CG RH+ +I D G V +GW YGQ G G D P+ V ++ G VA G
Sbjct: 403 VKRISCGARHTALIADNGKVFCWGWNKYGQLGLGDVIDRNIPSEV-TIEGCVAKNVACGW 461
Query: 1235 WHTVCISD 1258
WHT+ +++
Sbjct: 462 WHTLLLAE 469