BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00024772
         (1448 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABS72112.1| ankyrin repeat-rich protein [Solanum tuberosum]        377   e-159
ref|XP_002269428.1| PREDICTED: probable E3 ubiquitin-protein lig...   376   e-151
ref|XP_004149076.1| PREDICTED: probable E3 ubiquitin-protein lig...   361   e-147
ref|XP_002308433.1| predicted protein [Populus trichocarpa] gi|2...   371   e-147
ref|XP_003542295.1| PREDICTED: probable E3 ubiquitin-protein lig...   372   e-147

>gb|ABS72112.1| ankyrin repeat-rich protein [Solanum tuberosum]
          Length = 514

 Score =  377 bits (967), Expect(2) = e-159
 Identities = 182/229 (79%), Positives = 202/229 (88%)
 Frame = +1

Query: 1   RNSPLHYSAAQGHHEIVSLLLESGVDINLRNYRGQTALMQACQYGHWEVVLTLILFKANM 180
           RNSPLHYSAAQGHHEIVSLLLESGVDINLRNYRGQTALMQACQYGHWEVV  LILF AN+
Sbjct: 59  RNSPLHYSAAQGHHEIVSLLLESGVDINLRNYRGQTALMQACQYGHWEVVQILILFMANI 118

Query: 181 HRADYLNGGTALHLAALNGHSRCIRILLADYIPSVPSFCNILSKGSGTEKSFLESDQSSL 360
           H+ADYLNGGTALHLAALNGHSRCIR+LLADYIPS+P+FCN++ K S  E S  E D  +L
Sbjct: 119 HKADYLNGGTALHLAALNGHSRCIRLLLADYIPSIPNFCNVMRKRSRNEGSIQEFDDFAL 178

Query: 361 YQVINIASDGGITALHMAALNGHVESVHLLLDLGASVSEVTIEDGTTIDLIGAGSTPLHY 540
           ++VIN  +D GITALHMAALNGHVES+ LLLDLGASV++VT+EDGTTIDLIGAGSTPLHY
Sbjct: 179 HEVINRPADSGITALHMAALNGHVESLQLLLDLGASVTKVTVEDGTTIDLIGAGSTPLHY 238

Query: 541 AACGGNAVCCEILIAKGASLTAVNAKGWTPLTVAHSWHRESLEEILTPR 687
           AACGGNA CC++LIA+GASL A N  GWT L VA SWHR+ LEEIL+ R
Sbjct: 239 AACGGNAQCCQLLIARGASLNAENVNGWTSLMVARSWHRDGLEEILSTR 287



 Score =  213 bits (543), Expect(2) = e-159
 Identities = 115/222 (51%), Positives = 135/222 (60%), Gaps = 24/222 (10%)
 Frame = +2

Query: 695  PSPYLCLPFMSIVRIARECGWRSIDS---SCMDPCVLCLERKCTVAAEGCYHEFCTRCAL 865
            PSP+LCLP MSIV+IARECGWR+IDS   +C+DPCV+CLERKCTVAAEGC+HEFCTRCAL
Sbjct: 296  PSPFLCLPLMSIVKIARECGWRTIDSPSSTCLDPCVVCLERKCTVAAEGCFHEFCTRCAL 355

Query: 866  YLCSTNSTAIVAHGPQGSIPCPLCRHGIVSFIKLEATSPLVKDIARXXXXXXXXXXXALG 1045
            YLCST+ST+  AHGP GSIPCPLCRHGIVSF+KL  T P++K+ AR           A G
Sbjct: 356  YLCSTSSTSTAAHGPSGSIPCPLCRHGIVSFVKLADTMPIIKEAARTSLSLPFCSCTADG 415

Query: 1046 SEPNTLETPFCKPD---AQFPPTASSSFRXXXXXXXXXXXXXXXXXMGASDSNKSL---- 1204
             EP +LE P CKPD   ++  P   SSFR                 MG  D++ S     
Sbjct: 416  QEPTSLEAPLCKPDLYCSRLSP-LGSSFRSLSCQKFPALKFSPGLCMGTPDTSPSFVPRT 474

Query: 1205 -----------RSYRHSTSNSDQRGKSWLC---QCVAGSSEF 1288
                         +R STSN + R   WLC   Q V   S F
Sbjct: 475  GEREHLTRCSRPIFRRSTSNVEAR--RWLCSFSQSVETGSSF 514


>ref|XP_002269428.1| PREDICTED: probable E3 ubiquitin-protein ligase XBOS32 [Vitis
           vinifera] gi|297743747|emb|CBI36630.3| unnamed protein
           product [Vitis vinifera]
          Length = 509

 Score =  376 bits (966), Expect(2) = e-151
 Identities = 187/239 (78%), Positives = 206/239 (86%)
 Frame = +1

Query: 1   RNSPLHYSAAQGHHEIVSLLLESGVDINLRNYRGQTALMQACQYGHWEVVLTLILFKANM 180
           RNSPLHYSAAQGHHEIVSLLL SGVDINLRNYRGQTALMQACQ+GHWEVV TL+LF+AN+
Sbjct: 51  RNSPLHYSAAQGHHEIVSLLLVSGVDINLRNYRGQTALMQACQHGHWEVVQTLVLFRANI 110

Query: 181 HRADYLNGGTALHLAALNGHSRCIRILLADYIPSVPSFCNILSKGSGTEKSFLESDQSSL 360
           HRADYLNGGTALHLAALNGHSRCIR+LLADYIPS+P   NIL K S  E+S  E D+ +L
Sbjct: 111 HRADYLNGGTALHLAALNGHSRCIRLLLADYIPSIPDLWNILRKRSNREESISEFDEGAL 170

Query: 361 YQVINIASDGGITALHMAALNGHVESVHLLLDLGASVSEVTIEDGTTIDLIGAGSTPLHY 540
            QVIN  +DGGITALHMAALNGHVE++ LLLDLGAS+SEVT+EDGTTIDLIGAGSTPLHY
Sbjct: 171 CQVINRPADGGITALHMAALNGHVETLQLLLDLGASLSEVTVEDGTTIDLIGAGSTPLHY 230

Query: 541 AACGGNAVCCEILIAKGASLTAVNAKGWTPLTVAHSWHRESLEEILTPRSASFSILVSS 717
           AACGGNA CC+ILIA+GA L A NA GWTPL VA SWHR  LEEIL+ +    S  +SS
Sbjct: 231 AACGGNAQCCQILIARGAYLAAENANGWTPLMVARSWHRGWLEEILSTQPEVQSRTISS 289



 Score =  186 bits (472), Expect(2) = e-151
 Identities = 97/174 (55%), Positives = 114/174 (65%), Gaps = 5/174 (2%)
 Frame = +2

Query: 698  SPYLCLPFMSIVRIARECGWRSIDS--SCMDPCVLCLERKCTVAAEGCYHEFCTRCALYL 871
            S YL LP MSIV+IARECGWR+ DS  +C DPCV+CLERKCTVAA+GC HEFCT+CALYL
Sbjct: 289  SSYLSLPLMSIVKIARECGWRNNDSLPTCQDPCVVCLERKCTVAAQGCDHEFCTKCALYL 348

Query: 872  CSTNSTAIVAHGPQGSIPCPLCRHGIVSFIKLEATSPLVKDIARXXXXXXXXXXXALGSE 1051
            CS NS++ V+  P GSI CPLCR+GIVSF+KL  T PLVK+IAR             G E
Sbjct: 349  CSMNSSSTVSQCPLGSIACPLCRNGIVSFVKLPGTKPLVKEIARTSLSLSFCTCSGEGPE 408

Query: 1052 PNTLETPFCKPD---AQFPPTASSSFRXXXXXXXXXXXXXXXXXMGASDSNKSL 1204
            P +L TPFCKPD   ++  P   SSFR                 MGA D++ SL
Sbjct: 409  PTSLTTPFCKPDFHCSRISP-LGSSFRSLSCQRFSSVKLNSSLCMGAPDTSPSL 461


>ref|XP_004149076.1| PREDICTED: probable E3 ubiquitin-protein ligase XBOS32-like
           [Cucumis sativus] gi|449517425|ref|XP_004165746.1|
           PREDICTED: probable E3 ubiquitin-protein ligase
           XBOS32-like [Cucumis sativus]
          Length = 510

 Score =  361 bits (927), Expect(2) = e-147
 Identities = 176/227 (77%), Positives = 196/227 (86%)
 Frame = +1

Query: 1   RNSPLHYSAAQGHHEIVSLLLESGVDINLRNYRGQTALMQACQYGHWEVVLTLILFKANM 180
           RNSPLHYSAA GHHEIVSLLLESGV+INLRNYRGQTALMQACQ+GHWEVV TLILFKAN+
Sbjct: 51  RNSPLHYSAAHGHHEIVSLLLESGVEINLRNYRGQTALMQACQHGHWEVVQTLILFKANI 110

Query: 181 HRADYLNGGTALHLAALNGHSRCIRILLADYIPSVPSFCNILSKGSGTEKSFLESDQSSL 360
           HRADYLNGGTALHLAALNGHSRCIR+LLADY+PS P F N+L   S  E+   + +  +L
Sbjct: 111 HRADYLNGGTALHLAALNGHSRCIRLLLADYVPSTPKFWNMLHGSSNNEEQVSKFEFGAL 170

Query: 361 YQVINIASDGGITALHMAALNGHVESVHLLLDLGASVSEVTIEDGTTIDLIGAGSTPLHY 540
            +V+N  SDGGITALHMAALNGHV+SV LLLDLGAS  +VT+EDGTTIDLIGAGST LHY
Sbjct: 171 KEVVNRTSDGGITALHMAALNGHVDSVQLLLDLGASAFQVTVEDGTTIDLIGAGSTALHY 230

Query: 541 AACGGNAVCCEILIAKGASLTAVNAKGWTPLTVAHSWHRESLEEILT 681
           AACGGNA CC++LIA+GA LTA NA GWTPL VA SWHR+ L EIL+
Sbjct: 231 AACGGNAQCCQMLIARGADLTAQNANGWTPLMVARSWHRDWLVEILS 277



 Score =  190 bits (482), Expect(2) = e-147
 Identities = 90/135 (66%), Positives = 100/135 (74%), Gaps = 2/135 (1%)
 Frame = +2

Query: 689  LPPSPYLCLPFMSIVRIARECGWRSIDS--SCMDPCVLCLERKCTVAAEGCYHEFCTRCA 862
            L PSPYL LP MSIVRIARECGWRS DS  +C DPCV+CLER+CTVAA+GC HEFCTRCA
Sbjct: 287  LVPSPYLTLPLMSIVRIARECGWRSCDSLLTCQDPCVVCLERECTVAAQGCDHEFCTRCA 346

Query: 863  LYLCSTNSTAIVAHGPQGSIPCPLCRHGIVSFIKLEATSPLVKDIARXXXXXXXXXXXAL 1042
            LYLCSTN T+ V+HGP GSI CPLCR+GIVSFIKL  T P+ K+I R             
Sbjct: 347  LYLCSTNCTSSVSHGPTGSIACPLCRNGIVSFIKLPRTKPIAKEITRTSLSLSFCTCSGE 406

Query: 1043 GSEPNTLETPFCKPD 1087
              EP TL TP CKP+
Sbjct: 407  APEPPTLTTPLCKPE 421


>ref|XP_002308433.1| predicted protein [Populus trichocarpa] gi|222854409|gb|EEE91956.1|
           predicted protein [Populus trichocarpa]
          Length = 507

 Score =  371 bits (952), Expect(2) = e-147
 Identities = 189/239 (79%), Positives = 204/239 (85%)
 Frame = +1

Query: 1   RNSPLHYSAAQGHHEIVSLLLESGVDINLRNYRGQTALMQACQYGHWEVVLTLILFKANM 180
           RNSPLHYSAAQGHHEIVSLLLESGVDINLRNYRGQTALMQA Q+GHWEVVLTL+LFKAN+
Sbjct: 51  RNSPLHYSAAQGHHEIVSLLLESGVDINLRNYRGQTALMQAGQHGHWEVVLTLMLFKANI 110

Query: 181 HRADYLNGGTALHLAALNGHSRCIRILLADYIPSVPSFCNILSKGSGTEKSFLESDQSSL 360
           HRADYLNGGTALHLAALNGHSRCIR+LLADYIPS+    +ILSKGS    S  E D S+L
Sbjct: 111 HRADYLNGGTALHLAALNGHSRCIRLLLADYIPSIADCWDILSKGSRDNGSISEFDGSAL 170

Query: 361 YQVINIASDGGITALHMAALNGHVESVHLLLDLGASVSEVTIEDGTTIDLIGAGSTPLHY 540
            +VIN  +DGGITALHMAALNGHVESV LLLDLGAS  EVT+EDGTTIDLIGAGST LHY
Sbjct: 171 REVINRPADGGITALHMAALNGHVESVLLLLDLGASAYEVTVEDGTTIDLIGAGSTALHY 230

Query: 541 AACGGNAVCCEILIAKGASLTAVNAKGWTPLTVAHSWHRESLEEILTPRSASFSILVSS 717
           AACGGN  CC+ILIA+GASLTA NAKGWTPL VA SWHR  LE ILT +  + S +  S
Sbjct: 231 AACGGNPQCCQILIARGASLTAENAKGWTPLMVARSWHRNELEVILTTQQENQSQICPS 289



 Score =  179 bits (454), Expect(2) = e-147
 Identities = 105/210 (50%), Positives = 122/210 (58%), Gaps = 22/210 (10%)
 Frame = +2

Query: 695  PSPYLCLPFMSIVRIARECGWRSIDS--SCMDPCVLCLERKCTVAAEGCYHEFCTRCALY 868
            PSPY+ +PFMSIV+IARECGWR+ DS  +C D CV+CLE KCTVAAEGC HEFCTRCALY
Sbjct: 288  PSPYVSIPFMSIVKIARECGWRNNDSLPTCDDTCVVCLETKCTVAAEGCGHEFCTRCALY 347

Query: 869  LCSTNSTAIVAHGPQGSIPCPLCRHGIVSFIKLEATSPLVKDIARXXXXXXXXXXXALGS 1048
            LCST   + VA GP GS+ CPLCRHGIVSF+KL  T P+VK IAR             G 
Sbjct: 348  LCSTICISTVAQGPTGSVACPLCRHGIVSFVKLPGTKPVVKAIAR--TSLSLSFCTCTGE 405

Query: 1049 EPN--TLETPFCKPDAQFPP-TASSSFRXXXXXXXXXXXXXXXXXMGASDSNK------- 1198
            EP+  ++ TP  KPD Q    +  SSFR                 MGASD+         
Sbjct: 406  EPDFPSMTTPLRKPDFQCTKISPPSSFRSLSCQKFPSMNFNASRCMGASDTTPLVPCTID 465

Query: 1199 ----------SLRSYRHSTSNSDQRGKSWL 1258
                      S    R STSN ++R KSWL
Sbjct: 466  LNPREREVRCSRSRIRRSTSNPERR-KSWL 494


>ref|XP_003542295.1| PREDICTED: probable E3 ubiquitin-protein ligase XBOS32-like
           [Glycine max]
          Length = 508

 Score =  372 bits (955), Expect(2) = e-147
 Identities = 185/239 (77%), Positives = 206/239 (86%), Gaps = 1/239 (0%)
 Frame = +1

Query: 1   RNSPLHYSAAQGHHEIVSLLLESGVDINLRNYRGQTALMQACQYGHWEVVLTLILFKANM 180
           RNSPLHYSAA GHHEIV LLLESGVDINLRNYRGQTALMQACQ+GHWEVV TL++F AN+
Sbjct: 51  RNSPLHYSAAHGHHEIVYLLLESGVDINLRNYRGQTALMQACQHGHWEVVQTLVIFNANI 110

Query: 181 HRADYLNGGTALHLAALNGHSRCIRILLADYIPSVPSFCNILSKGSGTEKSFLESDQSSL 360
           H+ADYLNGGTALHLAALNGH+RCIR++LADYIPSVP+F N L   +G  KS  E DQS L
Sbjct: 111 HKADYLNGGTALHLAALNGHTRCIRLILADYIPSVPNFWNALQ--TGDHKSISEFDQSGL 168

Query: 361 YQVINIASDGGITALHMAALNGHVESVHLLLDLGASVSEVTIEDGTTIDLIGAGSTPLHY 540
            +VIN  +DGGITALHMAALNGHVESV LLLDLGASVSEVT+EDGTTIDLIG+GSTPLHY
Sbjct: 169 CEVINRTADGGITALHMAALNGHVESVQLLLDLGASVSEVTVEDGTTIDLIGSGSTPLHY 228

Query: 541 AACGGNAVCCEILIAKGASLTAVNAKGWTPLTVAHSWHRESLEEIL-TPRSASFSILVS 714
           AACGGN  CC++LIAKGA+LTA NA GWTPL VA SWHR+ LE+IL TP +    +L S
Sbjct: 229 AACGGNQQCCQLLIAKGANLTAENANGWTPLMVARSWHRDWLEDILKTPPADPLQVLPS 287



 Score =  177 bits (448), Expect(2) = e-147
 Identities = 96/211 (45%), Positives = 123/211 (58%), Gaps = 22/211 (10%)
 Frame = +2

Query: 695  PSPYLCLPFMSIVRIARECGWRSID-SSCMDPCVLCLERKCTVAAEGCYHEFCTRCALYL 871
            PSPY+ LP MSIVRIARECGWR+ D + C+DPC +CLERKCTVA EGC HEFCT+CALYL
Sbjct: 286  PSPYISLPLMSIVRIARECGWRTNDLAPCLDPCAVCLERKCTVAVEGCDHEFCTQCALYL 345

Query: 872  CSTNSTAIVAHGPQGSIPCPLCRHGIVSFIKLEATSPLVKDIARXXXXXXXXXXXA--LG 1045
            CSTNST+    GP GSI CPLCRHGIVSF+KL    PL K++ R           +  + 
Sbjct: 346  CSTNSTSTTTTGPPGSIACPLCRHGIVSFVKLPDARPLHKEMQRTSNLSLTFCTCSSEVL 405

Query: 1046 SEPNTLETPFCKPDAQFPPTA--SSSFRXXXXXXXXXXXXXXXXXMGASDS--------N 1195
             + + + TPFCKP ++    +  S SFR                 +GA  S         
Sbjct: 406  GDSSDMTTPFCKPTSRGSKNSSPSRSFRSISCQGFPSFRMNPSLCLGADVSPSLVPCTVG 465

Query: 1196 KSLR---------SYRHSTSNSDQRGKSWLC 1261
            +++R         S+R S+S +++R  SW C
Sbjct: 466  RNVRNHLARCSGSSFRRSSSQTERRKSSWFC 496