BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00024340
         (1536 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI37009.3| unnamed protein product [Vitis vinifera]              305   e-145
ref|XP_002272765.1| PREDICTED: tRNA guanosine-2'-O-methyltransfe...   304   e-145
ref|XP_002515211.1| conserved hypothetical protein [Ricinus comm...   295   e-141
ref|XP_003528332.1| PREDICTED: tRNA guanosine-2'-O-methyltransfe...   286   e-133
ref|XP_004158169.1| PREDICTED: tRNA guanosine-2'-O-methyltransfe...   281   e-131

>emb|CBI37009.3| unnamed protein product [Vitis vinifera]
          Length = 448

 Score =  305 bits (781), Expect(2) = e-145
 Identities = 144/231 (62%), Positives = 178/231 (77%)
 Frame = +1

Query: 13  KCTFWLPKKKRFCANLPLGDSLFCGNHTVRSNDKWIPCPVDPSHTVLEKNLEDHLKKCPF 192
           +C+FWLPKKKRFCAN  + DS+FCGNH  RS+ +W+PCP+DPSH+VL +NLE H+K+CP 
Sbjct: 4   RCSFWLPKKKRFCANFRINDSVFCGNHNTRSDAEWVPCPIDPSHSVLSENLEGHMKRCPL 63

Query: 193 LKYAKSLSLQPFYCKGINSGEEEIGVEDNVTSKMKRKAVYDMNACQISELISKIKFVHGL 372
           LK A+SLS QPFY KGIN+      +E N TS MKR AVY M   + S LISKI+ +H  
Sbjct: 64  LKQAQSLSSQPFYQKGINAAPTLASLE-NATSLMKRNAVYSMTVPEFSILISKIQSIHSS 122

Query: 373 ICSDIEDSHKVPESCAVWTNHEIDRKLPFQEKHVSQQTSILGNLEDIGVLKSPAADAQKK 552
           I +DI DS++VPE+C +W   E+DRKLPFQEKHV QQ SILGNLE+ GVL+  + D Q +
Sbjct: 123 IRNDIRDSYRVPEACDIWIKREVDRKLPFQEKHVVQQASILGNLEEFGVLEKSSGDDQME 182

Query: 553 QLSGNGSCVDDDDILAVVEFGAGRGYLTQVLADCYGIKKVFLVERRSYKLK 705
           Q   + S  DD+ + AVVEFGAGRGYLTQ+LADCYGIK+VFLVER+SYKLK
Sbjct: 183 QCDSDRSSGDDNGVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK 233



 Score =  239 bits (609), Expect(2) = e-145
 Identities = 115/194 (59%), Positives = 143/194 (73%), Gaps = 12/194 (6%)
 Frame = +3

Query: 705  VEDLNLNAVESLKGIPYLAIGKHICGPATDMTLRC-LENHGDQNGAAEGTANAYLRGLAL 881
            +EDLNL AVESL+G+PYLAIGKH+CGPATD++LRC L    +Q+ A +  +  YLRGLA+
Sbjct: 253  IEDLNLKAVESLQGVPYLAIGKHLCGPATDLSLRCCLAEESNQDDAVQCCSGHYLRGLAI 312

Query: 882  TTCCHHLCQWKNYINKRYMSHLGFDREYFHAMTWFTSWAVDATSHLDIIDDG-------- 1037
             TCCHHLCQWK+YINK+Y+ +LG  ++ FHA+TWFTSWAVDA    D+ D          
Sbjct: 313  ATCCHHLCQWKHYINKKYLMNLGITKDDFHAITWFTSWAVDADHGSDLSDVAGCRLHLQS 372

Query: 1038 -YSKDC--DACGAEGTLRNMSSAERAVLGFMCKDIIDMGRLMWIKEHGLYSRLVKYVPSN 1208
               K+C  D  G    ++NM + ERAV+GFMCK+IIDMGRLMW+KEHGL ++LVKYVP  
Sbjct: 373  IEKKECVEDVGGVAEIVQNMKAMERAVVGFMCKEIIDMGRLMWVKEHGLETQLVKYVPPT 432

Query: 1209 ISPENHLLIAAQKN 1250
            ISPENHLLIA   N
Sbjct: 433  ISPENHLLIAKHAN 446


>ref|XP_002272765.1| PREDICTED: tRNA guanosine-2'-O-methyltransferase TRM13 homolog
           [Vitis vinifera]
          Length = 465

 Score =  304 bits (779), Expect(2) = e-145
 Identities = 147/244 (60%), Positives = 182/244 (74%), Gaps = 13/244 (5%)
 Frame = +1

Query: 13  KCTFWLPKKKRFCANLPLGDSLFCGNHTVRSNDKWIPCPVDPSHTVLEKNLEDHLKKCPF 192
           +C+FWLPKKKRFCAN  + DS+FCGNH  RS+ +W+PCP+DPSH+VL +NLE H+K+CP 
Sbjct: 4   RCSFWLPKKKRFCANFRINDSVFCGNHNTRSDAEWVPCPIDPSHSVLSENLEGHMKRCPL 63

Query: 193 LKYAKSLSLQPFYCKGINSG--EEEIGVE-----------DNVTSKMKRKAVYDMNACQI 333
           LK A+SLS QPFY KGIN G  +E+ G E           +N TS MKR AVY M   + 
Sbjct: 64  LKQAQSLSSQPFYQKGINGGKDDEDEGDEKGAAAPTLASLENATSLMKRNAVYSMTVPEF 123

Query: 334 SELISKIKFVHGLICSDIEDSHKVPESCAVWTNHEIDRKLPFQEKHVSQQTSILGNLEDI 513
           S LISKI+ +H  I +DI DS++VPE+C +W   E+DRKLPFQEKHV QQ SILGNLE+ 
Sbjct: 124 SILISKIQSIHSSIRNDIRDSYRVPEACDIWIKREVDRKLPFQEKHVVQQASILGNLEEF 183

Query: 514 GVLKSPAADAQKKQLSGNGSCVDDDDILAVVEFGAGRGYLTQVLADCYGIKKVFLVERRS 693
           GVL+  + D Q +Q   + S  DD+ + AVVEFGAGRGYLTQ+LADCYGIK+VFLVER+S
Sbjct: 184 GVLEKSSGDDQMEQCDSDRSSGDDNGVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKS 243

Query: 694 YKLK 705
           YKLK
Sbjct: 244 YKLK 247



 Score =  238 bits (608), Expect(2) = e-145
 Identities = 115/197 (58%), Positives = 143/197 (72%), Gaps = 15/197 (7%)
 Frame = +3

Query: 705  VEDLNLNAVESLKGIPYLAIGKHICGPATDMTLRC-LENHGDQNGAAEGTANAYLRGLAL 881
            +EDLNL AVESL+G+PYLAIGKH+CGPATD++LRC L    +Q+ A +  +  YLRGLA+
Sbjct: 267  IEDLNLKAVESLQGVPYLAIGKHLCGPATDLSLRCCLAEESNQDDAVQCCSGHYLRGLAI 326

Query: 882  TTCCHHLCQWKNYINKRYMSHLGFDREYFHAMTWFTSWAVDATSHLDIID---------- 1031
             TCCHHLCQWK+YINK+Y+ +LG  ++ FHA+TWFTSWAVDA    D+ D          
Sbjct: 327  ATCCHHLCQWKHYINKKYLMNLGITKDDFHAITWFTSWAVDADHGSDLSDVAGIDILILS 386

Query: 1032 --DGYSKDC--DACGAEGTLRNMSSAERAVLGFMCKDIIDMGRLMWIKEHGLYSRLVKYV 1199
                  K+C  D  G    ++NM + ERAV+GFMCK+IIDMGRLMW+KEHGL ++LVKYV
Sbjct: 387  CCHSEKKECVEDVGGVAEIVQNMKAMERAVVGFMCKEIIDMGRLMWVKEHGLETQLVKYV 446

Query: 1200 PSNISPENHLLIAAQKN 1250
            P  ISPENHLLIA   N
Sbjct: 447  PPTISPENHLLIAKHAN 463


>ref|XP_002515211.1| conserved hypothetical protein [Ricinus communis]
           gi|223545691|gb|EEF47195.1| conserved hypothetical
           protein [Ricinus communis]
          Length = 438

 Score =  295 bits (756), Expect(2) = e-141
 Identities = 144/237 (60%), Positives = 177/237 (74%), Gaps = 2/237 (0%)
 Frame = +1

Query: 1   MTTTKCTFWLPKKKRFCANLPLGDSLFCGNHTVRSNDKWIPCPVDPSHTVLEKNLEDHLK 180
           M   +C FWLPKK R CAN PL DS FCGNHT R N +WIPCP+D SH+VL++NLE H+K
Sbjct: 1   MEEHRCKFWLPKKNRSCANTPLSDSPFCGNHTQRCNGQWIPCPIDSSHSVLKENLEGHIK 60

Query: 181 KCPFLKYAKSLSLQPFYCKGINSGEEEIGVEDNVTSKMKRKAVYDMNACQISELISKIKF 360
           +CP LK  +SLSLQPFY KGIN+G+E+   +D++TS+MKR AVY M   +  +LI KI+ 
Sbjct: 61  RCPLLKQTQSLSLQPFYQKGINAGKED---DDDITSEMKRNAVYSMTVSEFYKLIKKIES 117

Query: 361 VHGLICSDIEDSHKVPESCAVWTNHEIDRKLPFQEKHVSQQTSILGNLEDIGVLKSPAAD 540
           +H  +C+DI +S K+PE+C +W   EID +LPFQEKHV QQ +ILGNLED GVLKS    
Sbjct: 118 IHASLCNDILESFKLPEACNMWIKREIDSQLPFQEKHVKQQAAILGNLEDFGVLKSTL-- 175

Query: 541 AQKKQLSGNGSCVDDDD--ILAVVEFGAGRGYLTQVLADCYGIKKVFLVERRSYKLK 705
                  GN  C DD+   + AVVEFGAGRGYLTQ+LADCYGIK+VFLVER+SYKLK
Sbjct: 176 -------GNKVCDDDNANCVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK 225



 Score =  234 bits (596), Expect(2) = e-141
 Identities = 113/192 (58%), Positives = 139/192 (72%), Gaps = 10/192 (5%)
 Frame = +3

Query: 705  VEDLNLNAVESLKGIPYLAIGKHICGPATDMTLRC-LENHGDQNGAAEGTANAYLRGLAL 881
            +EDLNLNAVESL+G+PYLAIGKH+CGPATD+TLRC       ++     + N +LRGLA+
Sbjct: 245  IEDLNLNAVESLQGVPYLAIGKHLCGPATDLTLRCCFSKQSSEHNMGHCSDNDFLRGLAI 304

Query: 882  TTCCHHLCQWKNYINKRYMSHLGFDREYFHAMTWFTSWAVDATSHLDIIDDG-------Y 1040
             TCCHHLCQWK+YINK  ++ LG  +E FHA+TWFTSWAVDA    D+  DG        
Sbjct: 305  ATCCHHLCQWKHYINKNLIADLGITKEEFHAITWFTSWAVDADHGSDLSIDGRFHLQSME 364

Query: 1041 SKDC--DACGAEGTLRNMSSAERAVLGFMCKDIIDMGRLMWIKEHGLYSRLVKYVPSNIS 1214
             + C  DA G E  +RNM + +RA+LGFMCK IIDMGR++W KE GL ++LVKYVPS +S
Sbjct: 365  EEQCGGDADGVEDAVRNMKAVQRAILGFMCKQIIDMGRMIWAKECGLDAKLVKYVPSVVS 424

Query: 1215 PENHLLIAAQKN 1250
            PENHLLIA   N
Sbjct: 425  PENHLLIARHAN 436


>ref|XP_003528332.1| PREDICTED: tRNA guanosine-2'-O-methyltransferase TRM13 homolog
           [Glycine max]
          Length = 456

 Score =  286 bits (731), Expect(2) = e-133
 Identities = 141/250 (56%), Positives = 179/250 (71%), Gaps = 15/250 (6%)
 Frame = +1

Query: 1   MTTTKCTFWLPKKKRFCANLPLGDSLFCGNHTVRSNDKWIPCPVDPSHTVLEKNLEDHLK 180
           M T +C FWLP K RFCAN PL  SLFCGNH  RS  +WIPCP+DPSH+VL++NL+ H+K
Sbjct: 1   METKRCNFWLPNKNRFCANCPLNGSLFCGNHNSRSQGQWIPCPIDPSHSVLKQNLKGHVK 60

Query: 181 KCPFLKYAKSLSLQPFYCKGINSG----EEEIGVEDN------VTSKMKRKAVYDMNACQ 330
           +CP LK  +SLS QPFY KGIN+G    EE  GV+D+      V+S+MKR  +++M+  +
Sbjct: 61  RCPLLKQVQSLSDQPFYDKGINAGSDGEEETSGVDDSRLPTMTVSSEMKRNTLHEMSVAE 120

Query: 331 ISELISKIKFVHGLICSDIEDSHKVPESCAVWTNHEIDRKLPFQEKHVSQQTSILGNLED 510
              LI KI+F+H  +C DIEDS ++PE C +W     DRKLPFQEKH+ QQ SI+GNLE+
Sbjct: 121 FCNLIEKIEFLHESLCKDIEDSVQMPEVCRLWIKRVGDRKLPFQEKHIMQQASIVGNLEN 180

Query: 511 IGVLKSPAADAQKKQLSGNGSCVD-----DDDILAVVEFGAGRGYLTQVLADCYGIKKVF 675
            GVLK+     Q +       CV+     + D+LAV+EFGAGRGYLTQ+LADCYGIKKVF
Sbjct: 181 FGVLKNSLRRNQSE-------CVEPVEKKEKDVLAVIEFGAGRGYLTQMLADCYGIKKVF 233

Query: 676 LVERRSYKLK 705
           LVER++YKLK
Sbjct: 234 LVERKAYKLK 243



 Score =  219 bits (557), Expect(2) = e-133
 Identities = 108/194 (55%), Positives = 137/194 (70%), Gaps = 12/194 (6%)
 Frame = +3

Query: 705  VEDLNLNAVESLKGIPYLAIGKHICGPATDMTLRCLENHGDQNGAAEGTANAYLRGLALT 884
            +EDL+LNAVESL+G+PYLA GKH+CG ATD+TLRC      ++ + + TAN+   GLA+ 
Sbjct: 263  IEDLDLNAVESLQGVPYLATGKHLCGAATDLTLRCCFPEYREDSSGQYTANSKFGGLAIA 322

Query: 885  TCCHHLCQWKNYINKRYMSHLGFDREYFHAMTWFTSWAVDAT------------SHLDII 1028
            TCCHHLCQWK+YINKR+   LG  +E FHA+TWFTSWAVDA             SHL  I
Sbjct: 323  TCCHHLCQWKHYINKRFFLDLGLTKEEFHAITWFTSWAVDANHGSDLPDTTNCISHLQTI 382

Query: 1029 DDGYSKDCDACGAEGTLRNMSSAERAVLGFMCKDIIDMGRLMWIKEHGLYSRLVKYVPSN 1208
             +    +C A G E  L +M + +RA LGF CK IIDMGRLMW+++HGL ++LVK+VP +
Sbjct: 383  KE-QGDEC-ANGVEKILSDMQADKRAALGFKCKWIIDMGRLMWLRQHGLDAKLVKFVPPS 440

Query: 1209 ISPENHLLIAAQKN 1250
            ISPENHLL+A   N
Sbjct: 441  ISPENHLLLARPSN 454


>ref|XP_004158169.1| PREDICTED: tRNA guanosine-2'-O-methyltransferase TRM13 homolog
           [Cucumis sativus]
          Length = 464

 Score =  281 bits (719), Expect(2) = e-131
 Identities = 136/241 (56%), Positives = 180/241 (74%), Gaps = 9/241 (3%)
 Frame = +1

Query: 10  TKCTFWLPKKKRFCANLPLGDSLFCGNHTVRSNDKWIPCPVDPSHTVLEKNLEDHLKKCP 189
           ++C FWLPKK RFCA  P+ DS FCGNH+ R++   +PCPVDPSH+VL +NL+ H+K+CP
Sbjct: 3   SRCQFWLPKKNRFCAVAPINDSKFCGNHSSRADGDMVPCPVDPSHSVLRENLQGHVKRCP 62

Query: 190 FLKYAKSLSLQPFYCKGINSGEEE--------IGVEDNVTSKMKRKAVYDMNACQISELI 345
            LK  +SL+ QPFY KGIN+GEE+         G+ D+++S+MKR  VY M+  Q   L+
Sbjct: 63  LLKQTQSLAGQPFYQKGINAGEEDSMSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLL 122

Query: 346 SKIKFVHGLICSDIEDSHKVPESCAVWTNHEIDRKLPFQEKHVSQQTSILGNLEDIGVLK 525
            KI+ +H LIC DI+DS+K+ E+C +W   EIDRK+PFQEKHV QQ SILGNL++ GVL 
Sbjct: 123 GKIRALHDLICKDIQDSYKIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLS 182

Query: 526 SPAADAQKKQLSGNGSCVDDDDIL-AVVEFGAGRGYLTQVLADCYGIKKVFLVERRSYKL 702
           +     Q++   G+ S  +D +++ AVVEFGAGRGYLTQ+LADCYGIK+VFLVER+SYKL
Sbjct: 183 NYDGGEQRE---GDRSYGNDANVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKL 239

Query: 703 K 705
           K
Sbjct: 240 K 240



 Score =  216 bits (551), Expect(2) = e-131
 Identities = 109/190 (57%), Positives = 131/190 (68%), Gaps = 12/190 (6%)
 Frame = +3

Query: 705  VEDLNLNAVESLKGIPYLAIGKHICGPATDMTLRCLENHGDQNGAAEGTAN-AYLRGLAL 881
            +EDLNLNAVESL+  PYLAIGKH+CGPATD+ LRC       +   E   + + LRGLA+
Sbjct: 260  IEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAI 319

Query: 882  TTCCHHLCQWKNYINKRYMSHLGFDREYFHAMTWFTSWAVDATSHLDIID---------- 1031
             TCCHHLCQW +Y NKRY+  LG  +E F A+TWFTSWAVDA    DI D          
Sbjct: 320  ATCCHHLCQWNHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQS 379

Query: 1032 -DGYSKDCDACGAEGTLRNMSSAERAVLGFMCKDIIDMGRLMWIKEHGLYSRLVKYVPSN 1208
             +  S     C  +  +++M+  ERAVLGFMCK+IIDMGRLMW+KE GL ++LVKYVPS 
Sbjct: 380  SENESDGMIGCMVKDIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPST 439

Query: 1209 ISPENHLLIA 1238
            ISPENHLLIA
Sbjct: 440  ISPENHLLIA 449