BLASTX nr result
ID: Bupleurum21_contig00023973
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00023973
(1318 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAX16121.1| germacrene-D synthase [Actinidia deliciosa] 419 e-115
gb|ACO36239.1| (+)-valencene synthase [Vitis vinifera] 414 e-113
sp|Q6Q3H2.1|TPSVS_VITVI RecName: Full=Valencene synthase; Short=... 412 e-113
gb|ADR74199.1| gamma-cadinene synthase [Vitis vinifera] 412 e-112
ref|XP_003634696.1| PREDICTED: (-)-germacrene D synthase [Vitis ... 408 e-111
>gb|AAX16121.1| germacrene-D synthase [Actinidia deliciosa]
Length = 565
Score = 419 bits (1077), Expect = e-115
Identities = 203/439 (46%), Positives = 303/439 (69%)
Frame = +2
Query: 2 LVAAPGSKPAEKLKLIDTIERLGLSYHFRDQIEQQLEILFKAADDDNEDYDLFTTALRFR 181
++ A + ++ L L+D I+RLG+SYHF +IE L ++K D +D L T AL FR
Sbjct: 72 MLEAAAEQSSQMLNLVDKIQRLGVSYHFETEIETALRHIYKTCDYHFDD--LHTAALSFR 129
Query: 182 LFRQHGYGISCDVFNKFKDNTTGKFSESITSDALGMLSLYEATFLRCHGDDLLEEALAFT 361
L RQ GY +SCD+F+KFK N+ G+F ESI SD GMLSLYEAT LR HG+D+L+EALAFT
Sbjct: 130 LLRQQGYPVSCDMFDKFK-NSKGEFQESIISDVQGMLSLYEATCLRIHGEDILDEALAFT 188
Query: 362 TSHLKAMVSTTCSPFVAKQITHAFSQSLHRGIQRLEARFYISVYEEDPTKNEKLLRFAKL 541
+ L++ + +PF +QI HA +Q +H+G+ RL AR +I +E++ ++ LL FAKL
Sbjct: 189 ITQLRSALPNLSTPF-KEQIIHALNQPIHKGLTRLNARSHILFFEQNDCHSKDLLNFAKL 247
Query: 542 DFNLLMMIHKQELCQLTRWWDDLGLSSKLPYVRDRMVENYFWGVSIFSEPCYALGRIICT 721
DFNLL +H++ELC++TRWW DL + LP+ RDRMVE YFW + ++ EP Y L R + T
Sbjct: 248 DFNLLQKLHQRELCEITRWWKDLNFAKTLPFARDRMVECYFWILGVYFEPQYLLARRMLT 307
Query: 722 KMVAILAIXXXXXXXXXXFDELSLYTKAVEGFDISYMDQLPDYMQICYKTVLDVFDEFNE 901
K++A+++I +EL L+T A+E ++IS +DQLP+YM++CY+ +LDV+ +E
Sbjct: 308 KVIAMISIIDDIYDVYGTLEELVLFTDAIERWEISALDQLPEYMKLCYQALLDVYSMIDE 367
Query: 902 ELSKQGRLYNVDYLKESFKVTVRAYYKEAKWLNQKYVAPFEEYLAIATVSTGLYMLCFAT 1081
E++KQGR Y VDY K S K+ VRAY++EAKW +Q YV EEY+ +A V+ G ML ++
Sbjct: 368 EMAKQGRSYCVDYAKSSMKILVRAYFEEAKWFHQGYVPTMEEYMQVALVTAGYKMLATSS 427
Query: 1082 YTGISGEDASVEACNWAKKFPKIIESLGLVGRLNDDIWDYEDEKKREHVATSIRCYTKEH 1261
+ G+ G+ A+ EA +W P I+++ ++GRL DDI ++ E+KR HVA+++ CY+K+H
Sbjct: 428 FVGM-GDLATKEAFDWVSNDPLIVQAASVIGRLKDDIVGHKFEQKRGHVASAVECYSKQH 486
Query: 1262 GVGEDETRAELMRRIENAW 1318
G E+E EL +++ ++W
Sbjct: 487 GTTEEEAIIELDKQVTHSW 505
>gb|ACO36239.1| (+)-valencene synthase [Vitis vinifera]
Length = 556
Score = 414 bits (1064), Expect = e-113
Identities = 203/440 (46%), Positives = 299/440 (67%), Gaps = 1/440 (0%)
Frame = +2
Query: 2 LVAAPGSKPAEKLKLIDTIERLGLSYHFRDQIEQQLEILFKAADDDNE-DYDLFTTALRF 178
L AA + P++ L ID ++RLG++YHF +IE+ L+ + + D N+ D DL+ AL F
Sbjct: 62 LTAAAVANPSQLLNFIDAVQRLGVAYHFEQEIEEALQHICNSFHDCNDMDGDLYNIALGF 121
Query: 179 RLFRQHGYGISCDVFNKFKDNTTGKFSESITSDALGMLSLYEATFLRCHGDDLLEEALAF 358
RL RQ GY ISCD+FNKF D G+F E++ SD GML LYEA LR HG+D+L +ALAF
Sbjct: 122 RLLRQQGYTISCDIFNKFTDER-GRFKEALISDVRGMLGLYEAAHLRVHGEDILAKALAF 180
Query: 359 TTSHLKAMVSTTCSPFVAKQITHAFSQSLHRGIQRLEARFYISVYEEDPTKNEKLLRFAK 538
TT+HLKAMV + +A+Q+ HA ++ + +G++RLEAR+YISVY+++ ++ LL AK
Sbjct: 181 TTTHLKAMVESL-GYHLAEQVAHALNRPIRKGLERLEARWYISVYQDEAFHDKTLLELAK 239
Query: 539 LDFNLLMMIHKQELCQLTRWWDDLGLSSKLPYVRDRMVENYFWGVSIFSEPCYALGRIIC 718
LDFNL+ +HK+EL L RWW +L ++KLP+ RDR+VE YFW ++ EP Y GR I
Sbjct: 240 LDFNLVQSLHKEELSNLARWWKELDFATKLPFARDRLVEGYFWMHGVYFEPQYLRGRRIL 299
Query: 719 TKMVAILAIXXXXXXXXXXFDELSLYTKAVEGFDISYMDQLPDYMQICYKTVLDVFDEFN 898
TK++A+ +I +EL L+ +A+E +DI+ ++QLP+YM++CY +LDV+ E
Sbjct: 300 TKVIAMTSILDDIHDAYGTPEELKLFIEAIERWDINSINQLPEYMKLCYVALLDVYKEIE 359
Query: 899 EELSKQGRLYNVDYLKESFKVTVRAYYKEAKWLNQKYVAPFEEYLAIATVSTGLYMLCFA 1078
EE+ K+G Y V Y KE K VRAY+ EAKWL++++V FEEY+ +A S+G +L
Sbjct: 360 EEMEKEGNQYRVHYAKEVMKNQVRAYFAEAKWLHEEHVPAFEEYMRVALASSGYCLLATT 419
Query: 1079 TYTGISGEDASVEACNWAKKFPKIIESLGLVGRLNDDIWDYEDEKKREHVATSIRCYTKE 1258
++ G+ GE A+ EA +W PKI+ S + RL DDI ++ E+KR HVA+++ CY K+
Sbjct: 420 SFVGM-GEIATKEAFDWVTSDPKIMSSSNFITRLMDDIKSHKFEQKRGHVASAVECYMKQ 478
Query: 1259 HGVGEDETRAELMRRIENAW 1318
+GV E++ +E ++IENAW
Sbjct: 479 YGVSEEQVYSEFQKQIENAW 498
>sp|Q6Q3H2.1|TPSVS_VITVI RecName: Full=Valencene synthase; Short=VvVal; AltName:
Full=7-epi-alpha-selinene synthase
gi|45478055|gb|AAS66358.1| (+)-valencene synthase [Vitis
vinifera]
Length = 556
Score = 412 bits (1060), Expect = e-113
Identities = 202/440 (45%), Positives = 298/440 (67%), Gaps = 1/440 (0%)
Frame = +2
Query: 2 LVAAPGSKPAEKLKLIDTIERLGLSYHFRDQIEQQLEILFKAADDDNE-DYDLFTTALRF 178
L AA + P++ L ID ++RLG++YHF +IE+ L+ + + D N+ D DL+ AL F
Sbjct: 62 LTAAAVANPSQLLNFIDAVQRLGVAYHFEQEIEEALQHICNSFHDCNDMDGDLYNIALGF 121
Query: 179 RLFRQHGYGISCDVFNKFKDNTTGKFSESITSDALGMLSLYEATFLRCHGDDLLEEALAF 358
RL RQ GY ISCD+FNKF D G+F E++ SD GML LYEA LR HG+D+L +ALAF
Sbjct: 122 RLLRQQGYTISCDIFNKFTDER-GRFKEALISDVRGMLGLYEAAHLRVHGEDILAKALAF 180
Query: 359 TTSHLKAMVSTTCSPFVAKQITHAFSQSLHRGIQRLEARFYISVYEEDPTKNEKLLRFAK 538
TT+HLKAMV + +A+Q+ HA ++ + +G++RLEAR+YISVY+++ ++ LL AK
Sbjct: 181 TTTHLKAMVESL-GYHLAEQVAHALNRPIRKGLERLEARWYISVYQDEAFHDKTLLELAK 239
Query: 539 LDFNLLMMIHKQELCQLTRWWDDLGLSSKLPYVRDRMVENYFWGVSIFSEPCYALGRIIC 718
LDFNL+ +HK+EL L RWW +L ++KLP+ RDR+VE YFW ++ EP Y GR I
Sbjct: 240 LDFNLVQSLHKEELSNLARWWKELDFATKLPFARDRLVEGYFWMHGVYFEPQYLRGRRIL 299
Query: 719 TKMVAILAIXXXXXXXXXXFDELSLYTKAVEGFDISYMDQLPDYMQICYKTVLDVFDEFN 898
TK++A+ +I +EL L+ +A+E +DI+ ++QLP+YM++CY +LDV+ E
Sbjct: 300 TKVIAMTSILDDIHDAYGTPEELKLFIEAIERWDINSINQLPEYMKLCYVALLDVYKEIE 359
Query: 899 EELSKQGRLYNVDYLKESFKVTVRAYYKEAKWLNQKYVAPFEEYLAIATVSTGLYMLCFA 1078
EE+ K+G Y V Y KE K VRAY+ EAKWL++++V FEEY+ +A S+G +L
Sbjct: 360 EEMEKEGNQYRVHYAKEVMKNQVRAYFAEAKWLHEEHVPAFEEYMRVALASSGYCLLATT 419
Query: 1079 TYTGISGEDASVEACNWAKKFPKIIESLGLVGRLNDDIWDYEDEKKREHVATSIRCYTKE 1258
++ G+ GE A+ EA +W PKI+ S + RL DDI ++ E+KR HV +++ CY K+
Sbjct: 420 SFVGM-GEIATKEAFDWVTSDPKIMSSSNFITRLMDDIKSHKFEQKRGHVTSAVECYMKQ 478
Query: 1259 HGVGEDETRAELMRRIENAW 1318
+GV E++ +E ++IENAW
Sbjct: 479 YGVSEEQVYSEFQKQIENAW 498
>gb|ADR74199.1| gamma-cadinene synthase [Vitis vinifera]
Length = 585
Score = 412 bits (1058), Expect = e-112
Identities = 203/440 (46%), Positives = 296/440 (67%), Gaps = 1/440 (0%)
Frame = +2
Query: 2 LVAAPGSKPAEKLKLIDTIERLGLSYHFRDQIEQQLEILFKAADDDNE-DYDLFTTALRF 178
L AA + P++ L ID ++RLG++YHF +IE+ L+ + + D N+ D DL+ AL F
Sbjct: 91 LTAAAVANPSQLLNFIDAVQRLGVAYHFEQEIEEALQHICNSFHDCNDMDGDLYNIALGF 150
Query: 179 RLFRQHGYGISCDVFNKFKDNTTGKFSESITSDALGMLSLYEATFLRCHGDDLLEEALAF 358
RL RQ GY ISC +FNKF D G+F E++ SD GML LYEA LR HG+D+L +ALAF
Sbjct: 151 RLLRQQGYSISCGIFNKFMDER-GRFKEALISDVRGMLCLYEAAHLRVHGEDILAKALAF 209
Query: 359 TTSHLKAMVSTTCSPFVAKQITHAFSQSLHRGIQRLEARFYISVYEEDPTKNEKLLRFAK 538
TT+HLKAMV +A+Q+ HA ++ + +G++RLE R+YISVY+++ + LL AK
Sbjct: 210 TTTHLKAMVEGL-GYHLAEQVAHALNRPIRKGLERLEXRWYISVYQDEAFHDRTLLELAK 268
Query: 539 LDFNLLMMIHKQELCQLTRWWDDLGLSSKLPYVRDRMVENYFWGVSIFSEPCYALGRIIC 718
LDFNL+ +HK+EL L RWW +L ++KLP+ RDR+VE YFW ++ EP Y GR I
Sbjct: 269 LDFNLVQSLHKEELSNLARWWKELDFATKLPFARDRLVEGYFWMHGVYFEPQYLRGRRIL 328
Query: 719 TKMVAILAIXXXXXXXXXXFDELSLYTKAVEGFDISYMDQLPDYMQICYKTVLDVFDEFN 898
TK++A+ +I +EL L+ +A+E +DI+ +DQLP+YM++CY +LDV+ E
Sbjct: 329 TKVIAMTSILDDIHDAYGTPEELKLFIEAIERWDINSIDQLPEYMKLCYAALLDVYKEIE 388
Query: 899 EELSKQGRLYNVDYLKESFKVTVRAYYKEAKWLNQKYVAPFEEYLAIATVSTGLYMLCFA 1078
EE+ K+G + V Y KE K VRAY+ EAKWL++++V FEEY+ +A VS+G ML
Sbjct: 389 EEMEKEGNQHRVHYAKEVMKNQVRAYFAEAKWLHEEHVPTFEEYMRVALVSSGYCMLATT 448
Query: 1079 TYTGISGEDASVEACNWAKKFPKIIESLGLVGRLNDDIWDYEDEKKREHVATSIRCYTKE 1258
++ G+ GE A+ EA +W PKI+ S + RL DDI ++ E+KR HVA+++ CY K+
Sbjct: 449 SFVGM-GEIATNEAFDWVTSDPKIMSSSNFIARLMDDIKSHKFEQKRGHVASAVECYMKQ 507
Query: 1259 HGVGEDETRAELMRRIENAW 1318
+GV E++ +E ++IENAW
Sbjct: 508 YGVSEEQAYSEFQKQIENAW 527
>ref|XP_003634696.1| PREDICTED: (-)-germacrene D synthase [Vitis vinifera]
gi|302143188|emb|CBI20483.3| unnamed protein product
[Vitis vinifera]
Length = 557
Score = 408 bits (1048), Expect = e-111
Identities = 198/439 (45%), Positives = 297/439 (67%)
Frame = +2
Query: 2 LVAAPGSKPAEKLKLIDTIERLGLSYHFRDQIEQQLEILFKAADDDNEDYDLFTTALRFR 181
++ A A+KL LID I+RLG++YHF +I++ L+ +F + E+ D++T +LRFR
Sbjct: 64 MLMAADDDSAQKLLLIDAIQRLGVAYHFESEIDEVLKHMFDGSVVSAEE-DVYTASLRFR 122
Query: 182 LFRQHGYGISCDVFNKFKDNTTGKFSESITSDALGMLSLYEATFLRCHGDDLLEEALAFT 361
L RQ GY +SCD+FN FKDN G F ES++SD GMLSLYEAT R HG+D+L+EALAFT
Sbjct: 123 LLRQQGYHVSCDLFNNFKDNE-GNFKESLSSDVRGMLSLYEATHFRVHGEDILDEALAFT 181
Query: 362 TSHLKAMVSTTCSPFVAKQITHAFSQSLHRGIQRLEARFYISVYEEDPTKNEKLLRFAKL 541
T+HL++ + +P +A+Q+ HA Q + +G+ RLEAR Y SVY+ D + N+ LL+ AKL
Sbjct: 182 TTHLQSATKHSSNP-LAEQVVHALKQPIRKGLPRLEARHYFSVYQADDSHNKALLKLAKL 240
Query: 542 DFNLLMMIHKQELCQLTRWWDDLGLSSKLPYVRDRMVENYFWGVSIFSEPCYALGRIICT 721
DFNLL +H++EL ++ WW DL + KLP+ RDR+VE YFW + ++ EP + R I T
Sbjct: 241 DFNLLQKLHQKELSDISAWWKDLDFAHKLPFARDRVVECYFWILGVYFEPQFFFARRILT 300
Query: 722 KMVAILAIXXXXXXXXXXFDELSLYTKAVEGFDISYMDQLPDYMQICYKTVLDVFDEFNE 901
K++A+ +I +EL L+T+AVE +DIS +DQLP+YM++CY+ +L V+ E E
Sbjct: 301 KVIAMTSIIDDIYDVYGTLEELELFTEAVERWDISAIDQLPEYMRVCYQALLYVYSEIEE 360
Query: 902 ELSKQGRLYNVDYLKESFKVTVRAYYKEAKWLNQKYVAPFEEYLAIATVSTGLYMLCFAT 1081
E++K+GR Y + Y KE+ K VRAYY+EAKWL + + EEY+ +A V++ ML +
Sbjct: 361 EMAKEGRSYRLYYAKEAMKNQVRAYYEEAKWLQVQQIPTMEEYMPVALVTSAYSMLATTS 420
Query: 1082 YTGISGEDASVEACNWAKKFPKIIESLGLVGRLNDDIWDYEDEKKREHVATSIRCYTKEH 1261
+ G+ G+ + E+ +W PKI+ + +V RL DD+ ++ E+KR HVA+++ CY K+H
Sbjct: 421 FVGM-GDAVTKESFDWIFSKPKIVRASAIVCRLMDDMVSHKFEQKRGHVASAVECYMKQH 479
Query: 1262 GVGEDETRAELMRRIENAW 1318
G E ET E +++ +AW
Sbjct: 480 GASEQETHNEFHKQVRDAW 498