BLASTX nr result
ID: Bupleurum21_contig00023859
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00023859
(1390 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002269920.2| PREDICTED: DNA polymerase epsilon catalytic ... 434 e-180
ref|XP_002328167.1| predicted protein [Populus trichocarpa] gi|2... 391 e-166
ref|XP_002529065.1| DNA polymerase epsilon, catalytic subunit, p... 392 e-164
emb|CBI17056.3| unnamed protein product [Vitis vinifera] 376 e-159
ref|XP_003606227.1| DNA polymerase epsilon catalytic subunit A [... 350 e-150
>ref|XP_002269920.2| PREDICTED: DNA polymerase epsilon catalytic subunit A [Vitis
vinifera]
Length = 2214
Score = 434 bits (1116), Expect(2) = e-180
Identities = 214/316 (67%), Positives = 248/316 (78%), Gaps = 1/316 (0%)
Frame = +2
Query: 446 EDFRTNGKGSSVGPRPIVRNYEGRDESHRVKRSSQVNSSPYRTDHCQSAHELLTTQQQGE 625
EDFR G+ SS GPRPIVR YE + + NS +H +SA LT Q +
Sbjct: 1187 EDFRNKGRTSSAGPRPIVRCYEVNGKQQSDAMNCLANSPQEPANHSESA---LTPMQPTD 1243
Query: 626 ICMKDIDRNLDYQGWLDLKKRKWKEVRDKRKRRRLDNAKELNHTYNAAG-PSGGVNQKQA 802
I +IDRN+DYQGWL++KKRKWK+ R+KRKR+R+DN+K N T + P G +N KQA
Sbjct: 1244 ISTDNIDRNVDYQGWLEMKKRKWKDAREKRKRQRMDNSKTSNQTNGVSELPGGVINHKQA 1303
Query: 803 RGKPGVNSYFERQNLALTRCHWQIIQIEPSSQYGRFFAWVALQGVMHKIPINVPSVFYLN 982
+G+ GVNSYF R LALT +WQIIQ+ PSSQ+G+FFAWV ++GVM KIPI VP VFYLN
Sbjct: 1304 QGRTGVNSYFRRHELALTHSYWQIIQLAPSSQHGQFFAWVIVEGVMLKIPITVPRVFYLN 1363
Query: 983 TKAPVTEDFPGRRVNKILPHGHHNYNLIEVIIDEDQFLAESRKLAAHLADPEVEGIYETK 1162
+KA +TE+FPGRRVNK LPHGHH+YNLIE+IIDEDQF ESRKLAAHLADPEVEGIYETK
Sbjct: 1364 SKALITEEFPGRRVNKTLPHGHHSYNLIEIIIDEDQFRGESRKLAAHLADPEVEGIYETK 1423
Query: 1163 VPSEFNAVYKIGCVCKVDKSAEKRSAQDGWSLSELHMKTTTECSYLEHSFSFFYLYHSGS 1342
VP EFNAV +IGCVCKVDK+A+KR+ QDGWSLSELHMKTTTEC YLE S SFFYLYHS S
Sbjct: 1424 VPLEFNAVLQIGCVCKVDKTAKKRNIQDGWSLSELHMKTTTECPYLEQSISFFYLYHSIS 1483
Query: 1343 EGRAIYVGYFPASGTI 1390
EGRAIYVGYFPAS TI
Sbjct: 1484 EGRAIYVGYFPASRTI 1499
Score = 226 bits (576), Expect(2) = e-180
Identities = 107/143 (74%), Positives = 126/143 (88%)
Frame = +3
Query: 21 LRCQYIVACEPR*TPVSERAVPVAIFESDAELIRFYVKKWCKISSDVGIRSIIDWSYYKQ 200
LRCQYIVACEPR TPVSERAVPVAIFE+DAE+++FYV+KWCKISSDVGIRSI+DWSYYKQ
Sbjct: 1045 LRCQYIVACEPRGTPVSERAVPVAIFETDAEIMKFYVRKWCKISSDVGIRSIVDWSYYKQ 1104
Query: 201 RLCSAIQKIVTIPAAMQKVSNPVPRVIHPDWLHKKVREKEDKFRQRKLVDMFSSRNNDDA 380
RL SAIQKI+TIPAAMQKV+NPVPRV+HPDWL+KKVREKE+KFRQRKLVDMF S DD
Sbjct: 1105 RLGSAIQKIITIPAAMQKVANPVPRVVHPDWLYKKVREKEEKFRQRKLVDMFGSLKRDDN 1164
Query: 381 KELKAGDDSNNHGAVGQDIEDMK 449
++ ++NH ++IED++
Sbjct: 1165 VKMNVDAITSNHVISEENIEDLE 1187
>ref|XP_002328167.1| predicted protein [Populus trichocarpa] gi|222837682|gb|EEE76047.1|
predicted protein [Populus trichocarpa]
Length = 2221
Score = 391 bits (1004), Expect(2) = e-166
Identities = 197/317 (62%), Positives = 244/317 (76%), Gaps = 2/317 (0%)
Frame = +2
Query: 446 EDFRTNGKGSSVG-PRPIVRNYEGRDESHRVKRSSQVNSSPYRTDHCQSAHELLTTQQQG 622
EDF+ K SS+ PRPIVR Y+ +E K++ Q+NS+ +TD S HEL + Q
Sbjct: 1186 EDFQI--KSSSINRPRPIVRCYDV-NEKRSAKKTGQLNSAEQQTD---SVHELSSQPQHN 1239
Query: 623 EICMKDIDRNLDYQGWLDLKKRKWKEVRDKRKRRRLDNAKELNHTYNAAGPSGGV-NQKQ 799
++IDRN+DYQGWL+LKKRKWK++ D+RK++RL+N + +H +A+ GG+ N K
Sbjct: 1240 TSYAENIDRNVDYQGWLELKKRKWKDILDRRKKQRLNNTRTSHHITDASELLGGLTNHKG 1299
Query: 800 ARGKPGVNSYFERQNLALTRCHWQIIQIEPSSQYGRFFAWVALQGVMHKIPINVPSVFYL 979
A+ + GV SYF +ALTRCHWQIIQ+ PSS+ G+FFAWV ++G+M KIPI +P FYL
Sbjct: 1300 AQRRTGVGSYFTTHEVALTRCHWQIIQLLPSSRCGQFFAWVVVEGIMLKIPITIPRAFYL 1359
Query: 980 NTKAPVTEDFPGRRVNKILPHGHHNYNLIEVIIDEDQFLAESRKLAAHLADPEVEGIYET 1159
N KAP E+FPGRRV K LPHG +YNL+EVIIDED+F ES+KLAA LADPEVEGIYE+
Sbjct: 1360 NAKAPAMENFPGRRVTKTLPHGRPSYNLVEVIIDEDKFRKESKKLAALLADPEVEGIYES 1419
Query: 1160 KVPSEFNAVYKIGCVCKVDKSAEKRSAQDGWSLSELHMKTTTECSYLEHSFSFFYLYHSG 1339
KVP EFNAV +IGCVCKVDK+A+KR+AQDGWSLSELHMKTTTECSYLE S SFFY+YHS
Sbjct: 1420 KVPLEFNAVIQIGCVCKVDKTAKKRNAQDGWSLSELHMKTTTECSYLEQSISFFYIYHSI 1479
Query: 1340 SEGRAIYVGYFPASGTI 1390
SEGRAIYV Y PA GT+
Sbjct: 1480 SEGRAIYVIYLPALGTV 1496
Score = 221 bits (564), Expect(2) = e-166
Identities = 107/145 (73%), Positives = 128/145 (88%), Gaps = 1/145 (0%)
Frame = +3
Query: 21 LRCQYIVACEPR*TPVSERAVPVAIFESDAELIRFYVKKWCKISSDVGIRSIIDWSYYKQ 200
LRCQYIVACEPR TPVSERAVPVAIFE+D E+++FY++KWCK SSDVGIRSIIDWSYYKQ
Sbjct: 1046 LRCQYIVACEPRGTPVSERAVPVAIFETDPEIMKFYLRKWCKTSSDVGIRSIIDWSYYKQ 1105
Query: 201 RLCSAIQKIVTIPAAMQKVSNPVPRVIHPDWLHKKVREKEDKFRQRKLVDMFSSRNNDD- 377
RL SAIQKIVTIPAAMQKV+NPVPRV+HPDWLH+KVREKEDKFRQRKLVD+F+ R+ DD
Sbjct: 1106 RLGSAIQKIVTIPAAMQKVANPVPRVVHPDWLHRKVREKEDKFRQRKLVDIFNLRSRDDL 1165
Query: 378 AKELKAGDDSNNHGAVGQDIEDMKI 452
++++ +N+ A +D+ED +I
Sbjct: 1166 SRKMDDATITNHDEANVKDLEDFQI 1190
>ref|XP_002529065.1| DNA polymerase epsilon, catalytic subunit, putative [Ricinus
communis] gi|223531477|gb|EEF33309.1| DNA polymerase
epsilon, catalytic subunit, putative [Ricinus communis]
Length = 2227
Score = 392 bits (1007), Expect(2) = e-164
Identities = 194/316 (61%), Positives = 237/316 (75%), Gaps = 1/316 (0%)
Frame = +2
Query: 446 EDFRTNGKGSSVGPRPIVRNYEGRDESHRVKRSSQVNSSPYRTDHCQSAHELLTTQQQGE 625
EDFR N S+ GPRPIV Y E H VK + +++SS +TD+ +S HEL + QQ
Sbjct: 1189 EDFR-NKSISTNGPRPIVHCYATNSEEHSVKATGRMDSSHQQTDNGESVHELSSLVQQTA 1247
Query: 626 ICMKDIDRNLDYQGWLDLKKRKWKEVRDKRKRRRLDNAKELNHTYNAAGP-SGGVNQKQA 802
+ IDRN+DYQGWL+LKKRKWK+ ++RKR+RL N + +H P S +N+K+A
Sbjct: 1248 SSGESIDRNVDYQGWLELKKRKWKDTLERRKRQRLGNLRNSSHANGDFEPLSSVINRKKA 1307
Query: 803 RGKPGVNSYFERQNLALTRCHWQIIQIEPSSQYGRFFAWVALQGVMHKIPINVPSVFYLN 982
+ + GV SYF +ALTRCHWQIIQ+ P S G FFAWV ++G+M K+PI VP VFYLN
Sbjct: 1308 QSRTGVGSYFTTHEIALTRCHWQIIQLFPGSHCGEFFAWVVVEGIMLKVPITVPRVFYLN 1367
Query: 983 TKAPVTEDFPGRRVNKILPHGHHNYNLIEVIIDEDQFLAESRKLAAHLADPEVEGIYETK 1162
+KAP+TE FPGRRVNK LPHG H+YNL+EVIIDED+F ES+KLAA LADPEVEGIYETK
Sbjct: 1368 SKAPITEKFPGRRVNKTLPHGRHSYNLVEVIIDEDKFKKESKKLAALLADPEVEGIYETK 1427
Query: 1163 VPSEFNAVYKIGCVCKVDKSAEKRSAQDGWSLSELHMKTTTECSYLEHSFSFFYLYHSGS 1342
VP EFNA+ +IGCVCKVDK+A+KR+AQD WSL+EL MKTTTECSYLE S SFFYLY S S
Sbjct: 1428 VPLEFNAILQIGCVCKVDKTAKKRNAQDRWSLNELQMKTTTECSYLEQSISFFYLYQSIS 1487
Query: 1343 EGRAIYVGYFPASGTI 1390
EGRA+ V Y P T+
Sbjct: 1488 EGRAVVVVYLPFLRTV 1503
Score = 213 bits (542), Expect(2) = e-164
Identities = 100/143 (69%), Positives = 122/143 (85%)
Frame = +3
Query: 21 LRCQYIVACEPR*TPVSERAVPVAIFESDAELIRFYVKKWCKISSDVGIRSIIDWSYYKQ 200
LRCQYIVACEP+ TPVSERAVPVAIFE+DAE+++FY++KWCK S+D+GIRSIIDWSYYKQ
Sbjct: 1047 LRCQYIVACEPKGTPVSERAVPVAIFETDAEIMKFYLRKWCKTSTDIGIRSIIDWSYYKQ 1106
Query: 201 RLCSAIQKIVTIPAAMQKVSNPVPRVIHPDWLHKKVREKEDKFRQRKLVDMFSSRNNDDA 380
RL SAIQKI+TIPAAMQKV+NPVPRV+HPDWLHKKVREKEDKFRQRKLVD+F + +D
Sbjct: 1107 RLSSAIQKIITIPAAMQKVANPVPRVVHPDWLHKKVREKEDKFRQRKLVDIFRLSSKEDF 1166
Query: 381 KELKAGDDSNNHGAVGQDIEDMK 449
++N +++ED++
Sbjct: 1167 VRKNNDTITDNCMMDEENVEDLE 1189
>emb|CBI17056.3| unnamed protein product [Vitis vinifera]
Length = 2723
Score = 376 bits (966), Expect(2) = e-159
Identities = 198/310 (63%), Positives = 225/310 (72%), Gaps = 1/310 (0%)
Frame = +2
Query: 464 GKGSSVGPRPIVRNYEGRDESHRVKRSSQVNSSPYRTDHCQSAHELLTTQQQGEICMKDI 643
G SS GPRPIV+ +H +SA LT Q +I +I
Sbjct: 1631 GSTSSAGPRPIVQP----------------------ANHSESA---LTPMQPTDISTDNI 1665
Query: 644 DRNLDYQGWLDLKKRKWKEVRDKRKRRRL-DNAKELNHTYNAAGPSGGVNQKQARGKPGV 820
DRN+DYQGWL++KKRKWK+ R+KRKR+RL D+ L A G GV
Sbjct: 1666 DRNVDYQGWLEMKKRKWKDAREKRKRQRLLDDHIFLTFLVPAFLALG----------TGV 1715
Query: 821 NSYFERQNLALTRCHWQIIQIEPSSQYGRFFAWVALQGVMHKIPINVPSVFYLNTKAPVT 1000
NSYF R LALT +WQIIQ+ PSSQ+G+FFAWV ++GVM KIPI VP VFYLN+KA +T
Sbjct: 1716 NSYFRRHELALTHSYWQIIQLAPSSQHGQFFAWVIVEGVMLKIPITVPRVFYLNSKALIT 1775
Query: 1001 EDFPGRRVNKILPHGHHNYNLIEVIIDEDQFLAESRKLAAHLADPEVEGIYETKVPSEFN 1180
E+FPGRRVNK LPHGHH+YNLIE+IIDEDQF ESRKLAAHLADPEVEGIYETKVP EFN
Sbjct: 1776 EEFPGRRVNKTLPHGHHSYNLIEIIIDEDQFRGESRKLAAHLADPEVEGIYETKVPLEFN 1835
Query: 1181 AVYKIGCVCKVDKSAEKRSAQDGWSLSELHMKTTTECSYLEHSFSFFYLYHSGSEGRAIY 1360
AV +IGCVCKVDK+A+KR+ QDGWSLSELHMKTTTEC YLE S SFFYLYHS SEGRAIY
Sbjct: 1836 AVLQIGCVCKVDKTAKKRNIQDGWSLSELHMKTTTECPYLEQSISFFYLYHSISEGRAIY 1895
Query: 1361 VGYFPASGTI 1390
VGYFPAS TI
Sbjct: 1896 VGYFPASRTI 1905
Score = 214 bits (545), Expect(2) = e-159
Identities = 100/117 (85%), Positives = 113/117 (96%)
Frame = +3
Query: 21 LRCQYIVACEPR*TPVSERAVPVAIFESDAELIRFYVKKWCKISSDVGIRSIIDWSYYKQ 200
LRCQYIVACEPR TPVSERAVPVAIFE+DAE+++FYV+KWCKISSDVGIRSI+DWSYYKQ
Sbjct: 1519 LRCQYIVACEPRGTPVSERAVPVAIFETDAEIMKFYVRKWCKISSDVGIRSIVDWSYYKQ 1578
Query: 201 RLCSAIQKIVTIPAAMQKVSNPVPRVIHPDWLHKKVREKEDKFRQRKLVDMFSSRNN 371
RL SAIQKI+TIPAAMQKV+NPVPRV+HPDWL+KKVREKE+KFRQRKLVDMF S ++
Sbjct: 1579 RLGSAIQKIITIPAAMQKVANPVPRVVHPDWLYKKVREKEEKFRQRKLVDMFGSTSS 1635
>ref|XP_003606227.1| DNA polymerase epsilon catalytic subunit A [Medicago truncatula]
gi|355507282|gb|AES88424.1| DNA polymerase epsilon
catalytic subunit A [Medicago truncatula]
Length = 2248
Score = 350 bits (899), Expect(2) = e-150
Identities = 176/325 (54%), Positives = 226/325 (69%), Gaps = 10/325 (3%)
Frame = +2
Query: 446 EDFRTNGKGSSVGPRPIVRNYEGRDESHRVKRSSQVNSSPYRTDHCQSAHELLTTQQQGE 625
EDF G+ S++GPRPI+R+YE +E H K + Q +S D QQ E
Sbjct: 1201 EDFGNKGRSSTLGPRPIIRHYEANNEQHSEKFNGQEDSEQQHIDSSN-------IDQQNE 1253
Query: 626 ICMKDIDRNLDYQGWLDLKKRKWKEVRDKRKRRR-------LDNAKELNHTYNAAGPSGG 784
+++DR +DYQGWL LKKRKWK + KRK++R L+N+K+ +H G
Sbjct: 1254 TSCENVDRTVDYQGWLQLKKRKWKSMLGKRKKQRMSFDFLRLENSKKSDHLN---GSLEQ 1310
Query: 785 VNQKQARGKPGVNSYFERQNLALTRCHWQIIQIEPSSQYGRFFAWVALQGVMHKIPINVP 964
N K G+ V+SYF R + LTR HWQIIQ+ S Q G+FFAWV + G+M KIPI++P
Sbjct: 1311 TNSKMNHGRSNVSSYFRRHEVVLTRGHWQIIQLVHSPQVGQFFAWVVVDGIMLKIPISIP 1370
Query: 965 SVFYLNTKAPVTEDFPGRRVNKILPHGHHNYNLIEVI---IDEDQFLAESRKLAAHLADP 1135
VFYLN+++P TE+F G+RVNK LPHG H+YNL EV+ I+E Q+ ES+KLAA LADP
Sbjct: 1371 RVFYLNSRSP-TEEFSGKRVNKTLPHGRHSYNLYEVLVVTINEVQYKEESKKLAALLADP 1429
Query: 1136 EVEGIYETKVPSEFNAVYKIGCVCKVDKSAEKRSAQDGWSLSELHMKTTTECSYLEHSFS 1315
++EG+YETKVP EFNA+ ++GCVCKVDK+A+KRS Q+ W+L+ELHMKTTTEC+YLE S S
Sbjct: 1430 DIEGVYETKVPLEFNAIVQLGCVCKVDKTAKKRSLQEPWNLNELHMKTTTECAYLEQSIS 1489
Query: 1316 FFYLYHSGSEGRAIYVGYFPASGTI 1390
FYLYHS SEGRAIY GYFP S I
Sbjct: 1490 LFYLYHSISEGRAIYAGYFPGSQAI 1514
Score = 211 bits (536), Expect(2) = e-150
Identities = 100/143 (69%), Positives = 118/143 (82%)
Frame = +3
Query: 21 LRCQYIVACEPR*TPVSERAVPVAIFESDAELIRFYVKKWCKISSDVGIRSIIDWSYYKQ 200
LRCQYIVA EP+ TPVSERAVPVAIFE+DA++++FYV+KWCK+SSDVGIRSIIDWSYYKQ
Sbjct: 1059 LRCQYIVASEPKGTPVSERAVPVAIFETDADIMKFYVRKWCKVSSDVGIRSIIDWSYYKQ 1118
Query: 201 RLCSAIQKIVTIPAAMQKVSNPVPRVIHPDWLHKKVREKEDKFRQRKLVDMFSSRNNDDA 380
RL S+IQKI+TIPAAMQKV+NPVPRV+HPDWLHKKVREKEDKFRQRK+ D F S N ++
Sbjct: 1119 RLSSSIQKIITIPAAMQKVANPVPRVVHPDWLHKKVREKEDKFRQRKIDDTFKSMNKNEH 1178
Query: 381 KELKAGDDSNNHGAVGQDIEDMK 449
+ N V + DM+
Sbjct: 1179 SKKHNDSSGVNLVMVDDTVNDME 1201