BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00023630
         (1153 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002280446.1| PREDICTED: pectinesterase 2 [Vitis vinifera]      459   e-127
ref|XP_003517029.1| PREDICTED: pectinesterase 2-like [Glycine ma...   447   e-123
ref|XP_002320714.1| predicted protein [Populus trichocarpa] gi|2...   444   e-122
gb|AER38244.1| PME5 [Gossypium barbadense]                            443   e-122
ref|XP_002511679.1| Pectinesterase-2 precursor, putative [Ricinu...   442   e-122

>ref|XP_002280446.1| PREDICTED: pectinesterase 2 [Vitis vinifera]
          Length = 513

 Score =  459 bits (1182), Expect = e-127
 Identities = 228/338 (67%), Positives = 276/338 (81%), Gaps = 5/338 (1%)
 Frame = +1

Query: 154  VFGYSPSEVKKLCSETPNPKPCEYFLTNNPTYGALLKEPDFLQMSLKLALDRTLCADRNT 333
            V GY   +VK  CS+TP P+PCEYFL++ P +  + ++ DFL++S++LAL+R L A+ NT
Sbjct: 17   VSGYKSEDVKSWCSQTPYPQPCEYFLSHKPDHSPIKQKSDFLKISMQLALERALRAESNT 76

Query: 334  KSLGTKCRNKLEKAAWDDCVKLYENTIKKLNKTIGSDSKCTKEDAQTFLSTALTNLETCR 513
             SLG+KCRN+LEK AW DC+KLYE TI +LNKT+  ++KC++ D+QT+LSTALTNLETCR
Sbjct: 77   YSLGSKCRNELEKTAWSDCLKLYEYTILRLNKTVDPNTKCSQVDSQTWLSTALTNLETCR 136

Query: 514  AGFLELGVSDNLLPLMQNNVSMLISNTLAMNKGTYKKPSYKGKFPTWVTTGDRKLLQTAS 693
            AGF+ELGVSD LLPLM NNVS LISNTL++NK  Y +PSYK  FPTWV  GDRKLLQ++S
Sbjct: 137  AGFVELGVSDYLLPLMSNNVSKLISNTLSLNKVPYTEPSYKEGFPTWVKPGDRKLLQSSS 196

Query: 694  P--RANVVVAQDGSGNFKTLAAAVSAASKQSG-ARYVIYVKAGVYSENVEI--KTKNIMI 858
            P  +AN+VVA+DGSG++ T+ AAVSAASK+SG  RYVIYVKAG Y+EN+EI  K KNIM+
Sbjct: 197  PASQANIVVAKDGSGDYTTITAAVSAASKRSGTGRYVIYVKAGTYNENIEIGAKLKNIML 256

Query: 859  VGDGMGKTIFXXXXXXXXXXXXFNSATLAVVGDGFIGRAFTVRNTAGGQNHQAVALRSGS 1038
            +GDG+GKTI             FNSAT+A VGDGFIGR  T+RNTAG  NHQAVALRSGS
Sbjct: 257  LGDGIGKTIITGSKSVGGGSTTFNSATVAAVGDGFIGRGLTIRNTAGAANHQAVALRSGS 316

Query: 1039 DLSVFYQCSFEGYQDTLYVHSDRQFYRDCDIYGTVDFI 1152
            DLSVFYQCSFEGYQDTLYVHS+RQFYR+CDIYGTVDFI
Sbjct: 317  DLSVFYQCSFEGYQDTLYVHSERQFYRECDIYGTVDFI 354


>ref|XP_003517029.1| PREDICTED: pectinesterase 2-like [Glycine max]
            gi|356496348|ref|XP_003517030.1| PREDICTED:
            pectinesterase 2-like [Glycine max]
          Length = 515

 Score =  447 bits (1149), Expect = e-123
 Identities = 223/337 (66%), Positives = 268/337 (79%), Gaps = 4/337 (1%)
 Frame = +1

Query: 154  VFGYSPSEVKKLCSETPNPKPCEYFLTNNPTYG--ALLKEPDFLQMSLKLALDRTLCADR 327
            V GYS +EVK  C++TPNP+PCEYFL+NNPTY    L ++ DFL++SL+LA +R L    
Sbjct: 20   VSGYSWNEVKLWCNQTPNPQPCEYFLSNNPTYQYKPLKQKSDFLKLSLQLAQERALKGHE 79

Query: 328  NTKSLGTKCRNKLEKAAWDDCVKLYENTIKKLNKTIGSDSKCTKEDAQTFLSTALTNLET 507
            NT SLG+KCRN  E+ AW DCV+LYE TI+KLN+T+  ++K ++ DAQT+LSTALTNLET
Sbjct: 80   NTLSLGSKCRNPRERVAWADCVELYEQTIRKLNQTLKPNTKLSQVDAQTWLSTALTNLET 139

Query: 508  CRAGFLELGVSDNLLPLMQNNVSMLISNTLAMNKGTYKKPSYKGKFPTWVTTGDRKLLQT 687
            C+AGF ELGV D +LPLM NNV+ L+SNTLA+NK  Y++PSYK  FPTWV  GDRKLLQ 
Sbjct: 140  CKAGFYELGVQDYVLPLMSNNVTKLLSNTLALNKVPYQEPSYKEGFPTWVKPGDRKLLQA 199

Query: 688  ASP--RANVVVAQDGSGNFKTLAAAVSAASKQSGARYVIYVKAGVYSENVEIKTKNIMIV 861
            +SP  RANVVVA+DGSG F T++AA++AA K S  RYVIYVK GVY E VE+K KNIM+V
Sbjct: 200  SSPASRANVVVAKDGSGRFTTVSAAINAAPKSSSGRYVIYVKGGVYDEQVEVKAKNIMLV 259

Query: 862  GDGMGKTIFXXXXXXXXXXXXFNSATLAVVGDGFIGRAFTVRNTAGGQNHQAVALRSGSD 1041
            GDG+GKTI             F SAT+AVVGDGFI +  T RNTAG +NHQAVALRSGSD
Sbjct: 260  GDGIGKTIITGSKSVGGGTTTFRSATVAVVGDGFIAQGITFRNTAGAKNHQAVALRSGSD 319

Query: 1042 LSVFYQCSFEGYQDTLYVHSDRQFYRDCDIYGTVDFI 1152
            LSVFY+CSFEGYQDTLYVHS+RQFYR+C+IYGTVDFI
Sbjct: 320  LSVFYKCSFEGYQDTLYVHSERQFYRECNIYGTVDFI 356


>ref|XP_002320714.1| predicted protein [Populus trichocarpa] gi|222861487|gb|EEE99029.1|
            predicted protein [Populus trichocarpa]
          Length = 514

 Score =  444 bits (1143), Expect = e-122
 Identities = 223/340 (65%), Positives = 267/340 (78%), Gaps = 5/340 (1%)
 Frame = +1

Query: 148  PTVFGYSPSEVKKLCSETPNPKPCEYFLTNNPTYGALLKEPDFLQMSLKLALDRTLCADR 327
            PT+ GY+  EVK  CS+TPNP+PCEYFL++NP    +  E DFL++S++LALDR +    
Sbjct: 18   PTILGYNHDEVKSWCSKTPNPQPCEYFLSHNPKNTPIQHESDFLKISIELALDRAMHGKV 77

Query: 328  NTKSLGTKCRNKLEKAAWDDCVKLYENTIKKLNKTIGSDSKCTKEDAQTFLSTALTNLET 507
            NT SLG+KCRN LEKAAW+DC++LY+  +  LNKT GS  KCTK DAQT+LSTALTNLET
Sbjct: 78   NTYSLGSKCRNGLEKAAWEDCLELYQEIVLWLNKTTGS--KCTKYDAQTWLSTALTNLET 135

Query: 508  CRAGFLELGVSDNLLPLMQNNVSMLISNTLAMNKGTYKKPSYKGKFPTWVTTGDRKLLQT 687
            CR GF E G++D +LP+M NNVS LISNTLA+NK  Y +PS+ G FP+WV  GDRKLLQ+
Sbjct: 136  CRTGFAEFGMTDYILPMMSNNVSKLISNTLAINKAPYSEPSFNGGFPSWVRPGDRKLLQS 195

Query: 688  ASP--RANVVVAQDGSGNFKTLAAAVSAASKQSGA-RYVIYVKAGVYSENVEI--KTKNI 852
            +SP  +AN+VVAQDGSGN KT+  A+ AASK+SG+ RYVIYVKAG Y+ENVE+  K KN+
Sbjct: 196  SSPASQANIVVAQDGSGNVKTIKEAIVAASKRSGSGRYVIYVKAGTYNENVEVGQKVKNV 255

Query: 853  MIVGDGMGKTIFXXXXXXXXXXXXFNSATLAVVGDGFIGRAFTVRNTAGGQNHQAVALRS 1032
            M+VGDG+GKTI             F SAT AVVGD FI R  T RNTAG +NHQAVALRS
Sbjct: 256  MVVGDGIGKTIVTGSKSVGGGTTTFKSATFAVVGDNFIARDMTFRNTAGAKNHQAVALRS 315

Query: 1033 GSDLSVFYQCSFEGYQDTLYVHSDRQFYRDCDIYGTVDFI 1152
            GSDLSVFY+CSFEGYQDTLYVHS RQFYR+C+IYGTVDFI
Sbjct: 316  GSDLSVFYKCSFEGYQDTLYVHSQRQFYRECNIYGTVDFI 355


>gb|AER38244.1| PME5 [Gossypium barbadense]
          Length = 519

 Score =  443 bits (1140), Expect = e-122
 Identities = 224/340 (65%), Positives = 264/340 (77%), Gaps = 5/340 (1%)
 Frame = +1

Query: 148  PTVFGYSPSEVKKLCSETPNPKPCEYFLTNNPTYGALLKEPDFLQMSLKLALDRTLCADR 327
            P   GY  ++V+  C +TPNP+PCEYFL+++P       E  F ++   LAL+R   A+ 
Sbjct: 21   PVTLGYRANDVRSWCRKTPNPQPCEYFLSHDPKKTPTKDEFQFFKIPTHLALERAARAES 80

Query: 328  NTKSLGTKCRNKLEKAAWDDCVKLYENTIKKLNKTIGSDSKCTKEDAQTFLSTALTNLET 507
            NT SLGTKCR++ EKAAW DCV LYE TI +LNKT+ S +   K+DAQT+LSTALTNLET
Sbjct: 81   NTHSLGTKCRSEREKAAWSDCVNLYELTILRLNKTVDSGTNLNKDDAQTWLSTALTNLET 140

Query: 508  CRAGFLELGVSDNLLPLMQNNVSMLISNTLAMNKGTYKKPSYKGKFPTWVTTGDRKLLQT 687
            CR GF+ELGV D+LLP+M NNVS LISNTLA+NK  YK+P+YK  FPTWV  GDRKLLQ+
Sbjct: 141  CRTGFMELGVPDHLLPMMSNNVSQLISNTLALNKAPYKEPTYKDGFPTWVKPGDRKLLQS 200

Query: 688  ASP--RANVVVAQDGSGNFKTLAAAVSAASKQSGA-RYVIYVKAGVYSENVEI--KTKNI 852
            +SP   AN+VVAQDGSGN+KT+  A+SAASK+SG+ RYVIYVKAG Y ENVEI  K KNI
Sbjct: 201  SSPASTANIVVAQDGSGNYKTIKDAISAASKRSGSGRYVIYVKAGTYKENVEIGSKLKNI 260

Query: 853  MIVGDGMGKTIFXXXXXXXXXXXXFNSATLAVVGDGFIGRAFTVRNTAGGQNHQAVALRS 1032
            M+VGDG+GKTI             FNSAT+AVVGDGFI R  T RNTAG  NHQAVALRS
Sbjct: 261  MMVGDGIGKTIITGSKSVGGGSTTFNSATVAVVGDGFIARGITFRNTAGPTNHQAVALRS 320

Query: 1033 GSDLSVFYQCSFEGYQDTLYVHSDRQFYRDCDIYGTVDFI 1152
            GSDLSVFY+CSFEGYQDTLYVHS+RQFYR+CDIYGTVD+I
Sbjct: 321  GSDLSVFYKCSFEGYQDTLYVHSERQFYRECDIYGTVDWI 360


>ref|XP_002511679.1| Pectinesterase-2 precursor, putative [Ricinus communis]
            gi|223548859|gb|EEF50348.1| Pectinesterase-2 precursor,
            putative [Ricinus communis]
          Length = 514

 Score =  442 bits (1137), Expect = e-122
 Identities = 227/340 (66%), Positives = 267/340 (78%), Gaps = 5/340 (1%)
 Frame = +1

Query: 148  PTVFGYSPSEVKKLCSETPNPKPCEYFLTNNPTYGALLKEPDFLQMSLKLALDRTLCADR 327
            PTV GY+  EV+  CS+TPNP PCEYFL++NP    +  E DFL++S +LAL R + A  
Sbjct: 18   PTVLGYNHIEVQSWCSKTPNPGPCEYFLSHNPKNTPITHESDFLKISTELALQRAIHAQV 77

Query: 328  NTKSLGTKCRNKLEKAAWDDCVKLYENTIKKLNKTIGSDSKCTKEDAQTFLSTALTNLET 507
            NT SLGTKCRN+ EK AW DC++LYE  +  LN T  + SKCTK DAQT+LSTALTNLET
Sbjct: 78   NTYSLGTKCRNEREKTAWADCLELYEYAVLWLNHT--TTSKCTKYDAQTWLSTALTNLET 135

Query: 508  CRAGFLELGVSDNLLPLMQNNVSMLISNTLAMNKGTYKKPSYKGKFPTWVTTGDRKLLQT 687
            CR GF+ELGVSD +LPLM NNVS LISNTLA+N   Y++P+YKG FPTWV  GDRKLLQT
Sbjct: 136  CRTGFMELGVSDYVLPLMSNNVSKLISNTLAINNVPYEEPTYKGGFPTWVKPGDRKLLQT 195

Query: 688  ASP--RANVVVAQDGSGNFKTLAAAVSAASKQSGA-RYVIYVKAGVYSENVEI--KTKNI 852
             +P  +AN+VVA+DGSGNFKT+  AV+AASK+SG+ R++IYVKAGVY+ENVEI  K KN+
Sbjct: 196  TTPASQANIVVAKDGSGNFKTINEAVAAASKRSGSGRFIIYVKAGVYNENVEIGTKLKNL 255

Query: 853  MIVGDGMGKTIFXXXXXXXXXXXXFNSATLAVVGDGFIGRAFTVRNTAGGQNHQAVALRS 1032
            M VGDG+GKTI             F SAT AVVG+GFI R  T RNTAG +NHQAVALRS
Sbjct: 256  MFVGDGIGKTIVTGSKSVGGGATTFRSATFAVVGEGFIARDMTFRNTAGPENHQAVALRS 315

Query: 1033 GSDLSVFYQCSFEGYQDTLYVHSDRQFYRDCDIYGTVDFI 1152
            G+DLSVFY+CSFEGYQDTLYVHS RQFYR+CDIYGTVDFI
Sbjct: 316  GADLSVFYKCSFEGYQDTLYVHSQRQFYRECDIYGTVDFI 355